Method for preventing and controlling tissue culture seedling pollution

A technology of tissue culture seedling pollution and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of waste of manpower, material and financial resources, cumbersome steps, and reduced bactericidal effect, so as to reduce production costs and improve Economic benefit, the effect of the amount of seedlings used for remediation

CN101627727AInactive Publication Date: 2010-01-20SHANDONG YANTAI AGRI SCHOOL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2010-01-20
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to a method for preventing and controlling tissue culture seedling pollution by mercuric chloride. In the method for preventing and controlling tissue culture seedling pollution, the pollution of a polluted tissue culture seedling can be eliminated by a plurality of transfer periods, wherein each transfer period comprise the following two steps: (1) adding mercuric chloride into a culture medium of the tissue culture seedling; and (2) transferring the polluted tissue culture seedling on the culture medium containing the mercuric chloride. The mercuric chloride adopted by the invention is spectrum fungicide, has the effects of inhibition and sterilization on the polluted tissue culture seedling culture medium, has less dosage, can be directly added into the culture medium, carries out high pressure sterilization along with the culture medium and does not reduce the sterilizing effect; the pollution rate is reduced below 5 percent, the production cost can be effectively reduced, and economic benefits are improved; and as for some tissue culture seedlings rushing for a growing season, when the seedlings for production are polluted, the invention can eliminate the pollution, supplement the seedling dosage and reduce loss. In addition, the used culture medium carries out chemical processing so as to prevent pollution and be beneficial to environmental protection.
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Description

technical field

[0001] The invention relates to a method for preventing and controlling the pollution of tissue culture seedlings in plant tissue culture, in particular to a method for preventing and controlling the pollution of tissue culture seedlings by using mercuric chloride. Background technique

[0002] The industrialized production of tissue cultured seedlings has become an emerging industry with huge market potential, and is one of the important means of industrialized seedling raising technology for famous and high-quality plants. The adjustment of the physical and chemical environment can be planned in a short period of time to cultivate multiple excellent varieties with the same genetic characteristics. However, the pollution that occurs during the production of tissue cultured seedlings has led to an increase in production costs and has limited the rapid development of industrialized production of tissue cultured seedlings.

[0003] Contamination in plant tissu...

Examples

Embodiment 1-8

[0026] Embodiment 1-8: Removal of contamination of Gerbera chrysanthemum tissue culture seedlings

[0027] The tissue-cultured seedlings of gerbera were obtained from tissue-cultured gerbera receptacles, and bacterial plaques appeared around the roots of the contaminated tissue-cultured seedlings.

[0028] Add mercuric chloride to the culture medium of the tissue culture seedlings, transfer the tissue culture seedlings that are polluted during the production process of the tissue culture seedlings to the medium containing mercuric chloride through the normal transfer technology, Adjust the pH value of the tissue culture medium, control the culture temperature of the tissue culture, control the light conditions and light intensity, and then transfer the tissue culture to a new culture medium containing mercuric chloride after a switching cycle. Basically, cycle several transfer cycles in this way until the pollution of fine seedlings is eliminated.

[0029] The test result of ...

Embodiment 9-16

[0031] Example 9-16: Removal of Hemerocallis grandiflorum pollution

[0032] The tissue-cultured seedlings of Hemerocallis grandiflora were obtained from the pedicels of Hemerocallis grandiflora, and plaques appeared around the roots of the contaminated tissue-cultured seedlings.

[0033] Add mercuric chloride to the culture medium of the tissue culture seedlings, transfer the tissue culture seedlings that are polluted during the production process of the tissue culture seedlings to the medium containing mercuric chloride through the normal transfer technology, Adjust the pH value of the tissue culture medium, control the culture temperature of the tissue culture, control the light conditions and light intensity, and then transfer the tissue culture to a new culture medium containing mercuric chloride after a transfer cycle. Basically, repeat several transfer cycles in this way until the pollution of the tissue culture seedlings is eliminated.

[0034] The test result of tabl...

Embodiment 17-21

[0036] Embodiment 17-21: the removal of grape pollution

[0037] Tissue culture seedlings of grapes

[0038] Add mercuric chloride to the culture medium of the tissue culture seedlings, transfer the tissue culture seedlings that are polluted during the production process of the tissue culture seedlings to the medium containing mercuric chloride through the normal transfer technology, Adjust the pH value of the tissue culture medium, control the culture temperature of the tissue culture, control the light conditions and light intensity, and then transfer the tissue culture to a new culture medium containing mercuric chloride after a switching cycle. Basically, repeat several transfer cycles in this way until the pollution of the tissue culture seedlings is eliminated.

[0039] The test result of table 3 embodiment 17-21

[0040]

[0041] Among them, after the last transfer cycle, continue to observe Examples 8, 16 and 21, Example 8 was lethal on the 11th day, Exampl...