Preparation method of garlicin injection

Abstract

A garlicin injection for preventing and treating acute and chronic bacillary dysentery and enteritis, pertussis, fungus infection of lung and digestive tract, etc is prepared through ultrasonic treating to garlicin and tween-80, mixing with alcohol, ultrafiltration, pouring it in containers containing N2 in dark condition, sterilizing, and lamp examining.

Description

technical field

[0001] The present invention relates to a preparation method of antibacterial drug allicin injection, in particular, relates to a small-capacity injection mainly composed of allicin, after allicin is ultrasonically treated with Tween-80, and then mixed with ethanol , ultrafiltration to obtain allicin ultrafiltrate, prepared under light-proof conditions, ultrasonically treated, filled with nitrogen, finally sterilized, inspected by lights, packaged, and made into small-capacity injections, which belong to the field of chemical preparations. The product can be injected intravenously, has broad-spectrum antibacterial effect, and has inhibitory and killing effects on various cocci, pertussis bacillus, diphtheria bacillus, Shigella, typhoid and paratyphoid bacillus, Escherichia coli, Mycobacterium tuberculosis, etc.; has inhibitory effect on fungal infection It also has inhibitory and killing effects on amoeba, trichomonas vaginalis, and pinworms; it has the effect ...

Examples

Embodiment 1

[0027] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 2 hours, ultrasonicate for 10 minutes, and set aside.

[0028] B) Take the ethanol in the prescription and put it in a container for preparation, add the above-mentioned mixed solution with slow and fast stirring, stir for 10 minutes, and after ultrasonic treatment for 30 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 30,000 to obtain an ultrafiltrate .

[0029] C) Add the above-mentioned ultrafiltrate to water for injection by stirring slowly and quickly to 90% of the prepared amount, after ultrasonic treatment for 30 minutes, constant volume, adjust the pH value to 4.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, inspect by...

Embodiment 2

[0031] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 0.5 hour, ultrasonicate for 30 minutes, and set aside.

[0032] B) Take the ethanol in the prescription and place it in a container for preparation, add the above-mentioned mixed solution with slow and fast stirring, stir for 20 minutes, and after ultrasonic treatment for 10 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 20,000 to obtain an ultrafiltrate .

[0033] C) Add the above-mentioned ultrafiltrate to water for injection by stirring slowly and quickly to 80% of the preparation amount, after ultrasonic treatment for 10 minutes, constant volume, adjust the pH value to 5.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, insp...

Embodiment 3

[0035] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 1.5 hours, ultrasonicate for 15 minutes, and set aside.

[0036] B) Take the ethanol in the prescription and put it in a container for preparation, add the above mixed solution with slow and fast stirring, stir for 25 minutes, and after ultrasonic treatment for 15 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 10,000 to obtain an ultrafiltrate .

[0037] C) Add the above-mentioned ultrafiltrate to water for injection to 75% of the preparation amount in a slow and fast stirring manner, after ultrasonic treatment for 25 minutes, constant volume, adjust the pH value to 3.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, inspect by ...

technical field

[0001] The present invention relates to a preparation method of antibacterial drug allicin injection, in particular, relates to a small-capacity injection mainly composed of allicin, after allicin is ultrasonically treated with Tween-80, and then mixed with ethanol , ultrafiltration to obtain allicin ultrafiltrate, prepared under light-proof conditions, ultrasonically treated, filled with nitrogen, finally sterilized, inspected by lights, packaged, and made into small-capacity injections, which belong to the field of chemical preparations. The product can be injected intravenously, has broad-spectrum antibacterial effect, and has inhibitory and killing effects on various cocci, pertussis bacillus, diphtheria bacillus, Shigella, typhoid and paratyphoid bacillus, Escherichia coli, Mycobacterium tuberculosis, etc.; has inhibitory effect on fungal infection It also has inhibitory and killing effects on amoeba, trichomonas vaginalis, and pinworms; it has the effect ...

Examples

Embodiment 1

[0027] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 2 hours, ultrasonicate for 10 minutes, and set aside.

[0028] B) Take the ethanol in the prescription and put it in a container for preparation, add the above-mentioned mixed solution with slow and fast stirring, stir for 10 minutes, and after ultrasonic treatment for 30 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 30,000 to obtain an ultrafiltrate .

[0029] C) Add the above-mentioned ultrafiltrate to water for injection by stirring slowly and quickly to 90% of the prepared amount, after ultrasonic treatment for 30 minutes, constant volume, adjust the pH value to 4.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, inspect by...

Embodiment 2

[0031] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 0.5 hour, ultrasonicate for 30 minutes, and set aside.

[0032] B) Take the ethanol in the prescription and place it in a container for preparation, add the above-mentioned mixed solution with slow and fast stirring, stir for 20 minutes, and after ultrasonic treatment for 10 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 20,000 to obtain an ultrafiltrate .

[0033] C) Add the above-mentioned ultrafiltrate to water for injection by stirring slowly and quickly to 80% of the preparation amount, after ultrasonic treatment for 10 minutes, constant volume, adjust the pH value to 5.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, insp...

Embodiment 3

[0035] A) Take the allicin in the prescription and put it in a mortar, add the prescribed amount of Tween-80 while grinding, fully grind for 1.5 hours, ultrasonicate for 15 minutes, and set aside.

[0036] B) Take the ethanol in the prescription and put it in a container for preparation, add the above mixed solution with slow and fast stirring, stir for 25 minutes, and after ultrasonic treatment for 15 minutes, ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 10,000 to obtain an ultrafiltrate .

[0037] C) Add the above-mentioned ultrafiltrate to water for injection to 75% of the preparation amount in a slow and fast stirring manner, after ultrasonic treatment for 25 minutes, constant volume, adjust the pH value to 3.0, after the intermediate product is qualified, filter the medicinal solution through the terminal 0.22 μm After membrane sterilization and filtration, under the condition of avoiding light, fill with nitrogen, sterilize, inspect by ...

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