Method for preparing monodisperse silicon dioxide sphere by using blue algae as template

Abstract

The invention discloses a method for preparing silicon dioxide spheres by using blue algae as templates. The method comprises the following specific steps: cells of fresh blue algae are immobilized by using formaldehyde solution, a series of gradient dehydration by using 30 percent to 100 percent alcoholic solution is conducted to substitute for cell sap and organic coloring matter in cells of fresh blue algae, then orthosilicate ester, cosolvent monohydric alcohol, polyhydric alcohol or polymer alcohol, water and ammonia water are used to respectively conduct two-step reactions to synthesizesilicon dioxide spheres, the prepared silicon dioxide is dried and treated under high temperature, the blue algae templates are removed and large amounts of white monodisperse spherical silicon dioxide spheres with grain size of 0.5mum to 1.5mum. The prospect of the application in scientific fields such as analytical chemistry, medical science, biochemistry, electronic packaging materials and thelike is wide. The invention has the advantages that the operation is simple, the process can be easily controlled, the cycle is short, the environment is protected and the cost is low; and the product purity is high, the shape is regular, no hard aggregation exists and the industrial mass production is facilitated.

Description

technical field

[0001] The invention relates to a method for preparing monodisperse silica spheres using cyanobacteria as a template, in particular to a process for rapidly preparing monodisperse silica spheres using cyanobacteria as a template under normal temperature and pressure. Background technique

[0002] Cyanobacteria are very ancient microorganisms that are widely distributed in fresh water, sea water, inland salt water, wetlands, and deserts. Cyanobacteria can survive in high-temperature hot springs to alpine ice and snow, and there are more in warm water bodies with higher organic content. Cyanobacteria generally like high temperature and strong light, high pH and still water, low nitrogen and high phosphorus. Cyanobacteria mainly grow in fresh water and become important phytoplankton in fresh water. They often multiply in large numbers in warm seasons to form "water blooms", causing serious environmental pollution. Therefore, it is of great economic significanc...

Examples

Embodiment 1

[0030] 1) Processing of freshly collected cyanobacteria

[0031] 1. Centrifugal concentration: Collect fresh cyanobacteria by centrifugation. The rotating speed of the centrifuge is 2500 rpm, and the centrifuge is centrifuged for 10 minutes (adjust the rotating speed and time of the centrifuge according to the amount of algae sedimentation).

[0032] 2. Aldehyde fixation: fix cyanobacteria with formaldehyde solution.

[0033] 3. Cleaning: Wash the cyanobacteria soaked in the formaldehyde solution with double distilled water, and wash it several times, about 5 minutes each time, to remove the residual solvent on the surface.

[0034] 4. Dehydration: Collect the cleaned cyanobacteria by centrifugation, centrifuge at a speed of 2500 rpm, and centrifuge for 10 minutes. The cyanobacteria were dehydrated with gradients from 30% to 100% alcohol solution, about 20 minutes each time. Use 100% alcohol to dehydrate twice, and the dehydration time is about 20 minutes each time.

[003...

Embodiment 2

[0042] 1) Processing of freshly collected cyanobacteria

[0043] 1. Centrifugal concentration: Collect fresh cyanobacteria by centrifugation. The rotating speed of centrifuge is 3000 rev / min, centrifugation 10min.

[0044] 2. Aldehyde fixation: fix cyanobacteria with formaldehyde solution.

[0045] 3. Cleaning: Wash the cyanobacteria soaked in the formaldehyde solution with double distilled water, and wash it several times, about 10 minutes each time, to remove the residual formaldehyde solution on the surface.

[0046] 4. Dehydration: Centrifuge to collect the cleaned cyanobacteria, centrifuge at a speed of 3000 rpm, and centrifuge for 10 minutes. Gradient dehydration was carried out with 30% to 100% alcohol solution, each time about 30 minutes. Use 100% alcohol to dehydrate twice, and the dehydration time is about 30 minutes each time.

[0047] 2) Preparation of monodisperse silica microspheres using treated cyanobacteria as a template

[0048] Add absolute ethanol, doubl...

Embodiment 3

[0053] The difference between this embodiment and the specific example 1 is: the process of preparing monodisperse silica microspheres using the treated cyanobacteria as a template.

[0054] Add absolute ethanol, double-distilled water, and ammonia water into the centrifuge tube at a volume ratio of 5:5:2, and mix evenly to form solution I. Put tetraethyl orthosilicate and ethanol co-solvent at a volume ratio of 4:5 in the centrifuge tube After uniform mixing in , solution II was formed. This preparation process is carried out in two steps:

[0055] The first infiltration: take 24 milliliters of the above-mentioned solution I with a centrifuge tube, put the treated cyanobacteria into a centrifuge tube filled with solution I, put the centrifuge tube in a constant temperature oscillator, and shake it at a low speed. Permeate at 25°C for 45h. After the infiltration at this stage is over, the infiltrated cyanobacteria are washed with double distilled water to remove excess solve...

technical field

[0001] The invention relates to a method for preparing monodisperse silica spheres using cyanobacteria as a template, in particular to a process for rapidly preparing monodisperse silica spheres using cyanobacteria as a template under normal temperature and pressure. Background technique

[0002] Cyanobacteria are very ancient microorganisms that are widely distributed in fresh water, sea water, inland salt water, wetlands, and deserts. Cyanobacteria can survive in high-temperature hot springs to alpine ice and snow, and there are more in warm water bodies with higher organic content. Cyanobacteria generally like high temperature and strong light, high pH and still water, low nitrogen and high phosphorus. Cyanobacteria mainly grow in fresh water and become important phytoplankton in fresh water. They often multiply in large numbers in warm seasons to form "water blooms", causing serious environmental pollution. Therefore, it is of great economic significanc...

Examples

Embodiment 1

[0030] 1) Processing of freshly collected cyanobacteria

[0031] 1. Centrifugal concentration: Collect fresh cyanobacteria by centrifugation. The rotating speed of the centrifuge is 2500 rpm, and the centrifuge is centrifuged for 10 minutes (adjust the rotating speed and time of the centrifuge according to the amount of algae sedimentation).

[0032] 2. Aldehyde fixation: fix cyanobacteria with formaldehyde solution.

[0033] 3. Cleaning: Wash the cyanobacteria soaked in the formaldehyde solution with double distilled water, and wash it several times, about 5 minutes each time, to remove the residual solvent on the surface.

[0034] 4. Dehydration: Collect the cleaned cyanobacteria by centrifugation, centrifuge at a speed of 2500 rpm, and centrifuge for 10 minutes. The cyanobacteria were dehydrated with gradients from 30% to 100% alcohol solution, about 20 minutes each time. Use 100% alcohol to dehydrate twice, and the dehydration time is about 20 minutes each time.

[003...

Embodiment 2

[0042] 1) Processing of freshly collected cyanobacteria

[0043] 1. Centrifugal concentration: Collect fresh cyanobacteria by centrifugation. The rotating speed of centrifuge is 3000 rev / min, centrifugation 10min.

[0044] 2. Aldehyde fixation: fix cyanobacteria with formaldehyde solution.

[0045] 3. Cleaning: Wash the cyanobacteria soaked in the formaldehyde solution with double distilled water, and wash it several times, about 10 minutes each time, to remove the residual formaldehyde solution on the surface.

[0046] 4. Dehydration: Centrifuge to collect the cleaned cyanobacteria, centrifuge at a speed of 3000 rpm, and centrifuge for 10 minutes. Gradient dehydration was carried out with 30% to 100% alcohol solution, each time about 30 minutes. Use 100% alcohol to dehydrate twice, and the dehydration time is about 30 minutes each time.

[0047] 2) Preparation of monodisperse silica microspheres using treated cyanobacteria as a template

[0048] Add absolute ethanol, doubl...

Embodiment 3

[0053] The difference between this embodiment and the specific example 1 is: the process of preparing monodisperse silica microspheres using the treated cyanobacteria as a template.

[0054] Add absolute ethanol, double-distilled water, and ammonia water into the centrifuge tube at a volume ratio of 5:5:2, and mix evenly to form solution I. Put tetraethyl orthosilicate and ethanol co-solvent at a volume ratio of 4:5 in the centrifuge tube After uniform mixing in , solution II was formed. This preparation process is carried out in two steps:

[0055] The first infiltration: take 24 milliliters of the above-mentioned solution I with a centrifuge tube, put the treated cyanobacteria into a centrifuge tube filled with solution I, put the centrifuge tube in a constant temperature oscillator, and shake it at a low speed. Permeate at 25°C for 45h. After the infiltration at this stage is over, the infiltrated cyanobacteria are washed with double distilled water to remove excess solve...

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