Method for domesticating pleurotus eryngii female parent

Abstract

The invention relates to a method for domesticating a pleurotus eryngii female parent, comprising the following steps of: (1) activating stored female parents; (2) inoculating the female parents onto multiple water agar flat plates, culturing for 8-12 days at the temperature of 20-25 DEG C, then screening out the flat plates on which mycelia grow well, and eliminating other flat plates; (3) inoculating the screened strains onto multiple PDA (potato dextrose agar medium) flat plates, culturing for 8-12 days at the temperature of 18-26 DEG C, then screening out the flat plates on which the mycelia grow well, and eliminating other flat plates; and (4) inoculating the screened strains into multiple PDA test tubes, culturing for 8-12 days at the temperature of 18-26 DEG C, then screening out the test tubes in which the mycelia grow well, extracting the front end of the mycelia in the test tubes, then transferring the front end of the mycelia in the test tubes into the PDA test tubes, and selecting one PDA test tube with the best performance to be preserved as the female parent. The female parent domesticated by adopting the method disclosed by the invention has the characteristics of vigorous growth, high stress resistance and high stability.

Description

technical field

[0001] The invention relates to the field of edible fungi, in particular to a domestication method of the parent species of Pleurotus eryngii. Background technique

[0002] Pleurotus eryngii (DC.ex Fr) Quél, also known as Pleurotus eryngii (DC.ex Fr) Quél, is a high-quality large-scale fleshy toadstool, with solitary or group fruiting bodies, slow umbrella opening, and stipe The tissue is fine and firm, white and strong, with few spores, long fresh-keeping period, crisp and tender texture, unique flavor, and special almond fragrance. Pleurotus eryngii has high nutritional value, medicinal value and economic value. In recent years, domestic Pleurotus eryngii production has developed very rapidly, and has changed from seasonal cultivation to factory cultivation. Factory cultivation can more accurately control temperature, light, humidity, CO 2 Concentration and other environmental conditions make the yield and quality more stable, but industrial cultivation r...

Examples

Embodiment 1

[0020] Select a better mother test tube from the preserved mother test tubes for activation (temperature is 22-24°C), the culture period is 3 days, connect it on 15 water agar plates, and place it in a constant temperature incubator at 20°C to 25°C. After culturing at ℃ for 10 days, select plates with no missing corners and well-developed hyphae in the base for preservation, and eliminate other plates with a rate of elimination of more than 80%. Inoculate the screened strains on 10 PDA plates, and culture them in a constant temperature incubator at 20°C to 25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and consistent growth for preservation. Elimination, the elimination rate is over 80%. Connect them to 12 PDA test tubes (barren), and culture them for 10 days in a constant temperature incubator at 20°C to 25°C. Select a test tube with no missing corners, well-developed hyphae in the base, and consistent growth, take the front ...

Embodiment 2

[0022] Select a better mother test tube from the preserved mother test tubes for activation (temperature is 22-24°C), the culture period is 2 days, connect it on 10 water agar (barren) plates, and place it in a constant temperature incubator for 20 After culturing for 10 days under the condition of ℃~25℃, select the plate with no edge loss and more developed hyphae in the base for preservation, and eliminate the other plates, with a rate of elimination of more than 80%. Inoculate the screened strains on 10 nutrient-rich PDA plates and culture them in a constant temperature incubator at 20°C to 25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and uniform growth Save, other tablets are eliminated, and the elimination rate is over 80%. Connect it to 12 PDA test tubes and culture it for 10 days in a constant temperature incubator at 20°C to 25°C. Select a test tube with no missing corners, well-developed hyphae in the base, and cons...

technical field

[0001] The invention relates to the field of edible fungi, in particular to a domestication method of the parent species of Pleurotus eryngii. Background technique

[0002] Pleurotus eryngii (DC.ex Fr) Quél, also known as Pleurotus eryngii (DC.ex Fr) Quél, is a high-quality large-scale fleshy toadstool, with solitary or group fruiting bodies, slow umbrella opening, and stipe The tissue is fine and firm, white and strong, with few spores, long fresh-keeping period, crisp and tender texture, unique flavor, and special almond fragrance. Pleurotus eryngii has high nutritional value, medicinal value and economic value. In recent years, domestic Pleurotus eryngii production has developed very rapidly, and has changed from seasonal cultivation to factory cultivation. Factory cultivation can more accurately control temperature, light, humidity, CO 2 Concentration and other environmental conditions make the yield and quality more stable, but industrial cultivation r...

Examples

Embodiment 1

[0020] Select a better mother test tube from the preserved mother test tubes for activation (temperature is 22-24°C), the culture period is 3 days, connect it on 15 water agar plates, and place it in a constant temperature incubator at 20°C to 25°C. After culturing at ℃ for 10 days, select plates with no missing corners and well-developed hyphae in the base for preservation, and eliminate other plates with a rate of elimination of more than 80%. Inoculate the screened strains on 10 PDA plates, and culture them in a constant temperature incubator at 20°C to 25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and consistent growth for preservation. Elimination, the elimination rate is over 80%. Connect them to 12 PDA test tubes (barren), and culture them for 10 days in a constant temperature incubator at 20°C to 25°C. Select a test tube with no missing corners, well-developed hyphae in the base, and consistent growth, take the front ...

Embodiment 2

[0022] Select a better mother test tube from the preserved mother test tubes for activation (temperature is 22-24°C), the culture period is 2 days, connect it on 10 water agar (barren) plates, and place it in a constant temperature incubator for 20 After culturing for 10 days under the condition of ℃~25℃, select the plate with no edge loss and more developed hyphae in the base for preservation, and eliminate the other plates, with a rate of elimination of more than 80%. Inoculate the screened strains on 10 nutrient-rich PDA plates and culture them in a constant temperature incubator at 20°C to 25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and uniform growth Save, other tablets are eliminated, and the elimination rate is over 80%. Connect it to 12 PDA test tubes and culture it for 10 days in a constant temperature incubator at 20°C to 25°C. Select a test tube with no missing corners, well-developed hyphae in the base, and cons...