Method for acclimating mother seeds of hypsizygus marmoreus

Abstract

A method for acclimating mother seeds of hypsizygus marmoreus comprises the following steps of adopting a better mother seed tube to activate; inoculating on 10-15 agar (barren) plates; after breeding, inoculating the strains which are optimally screened on a PDA plate; after breeding, optimally inoculating the plate in a PDA tube; after breeding, optimally inoculating to the PDA tube; and adopting the best one as the mother seed to store. The invention has the following beneficial effects: fast growth speed of the hypha, short growth period, early fruiting time, strong ability for resisting microorganisms and good quality.

Description

technical field

[0001] The invention relates to a domestication method of the mother species of shimeji mushroom. Background technique

[0002] True jiji mushroom is a nutritious edible and medicinal rare edible fungus. The hot water and organic solvent extracts of its fruiting bodies have the effects of eliminating free radicals in the body, improving human immunity, and preventing aging. At present, the commercial cultivation of Shiji mushroom is one of the important varieties of industrial production of edible fungi. However, due to the long service life of the strains, the strains are prone to aging, and the aged mycelia have fewer growth points and weaker vigor, so the produced shimeji mushrooms will have weak anti-associated bacteria, poor stability, reduced growth vigor, and poor quality. Poor and yield reduction and so on. Strain domestication refers to the method of gradually adapting edible fungi to a certain condition through artificial measures, and the method ...

Examples

Embodiment 1

[0014] Select a relatively good mother species test tube for activation, the period is 3 days, connect it to about 15 water agar plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed silk are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on PDA plates and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and consistent growth for preservation, and eliminate other plates. The elimination rate is over 80%. Connect it to a PDA test tube (barren), and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. Select a test tube with no missing corners, well-developed hyphae in the base, and consistent growth, take the front end, and then transfer it to a PDA test tube, and select the o...

Embodiment 2

[0016] Select a relatively good mother seed test tube for activation, the period is 2 days, connect it on about 12 water agar (barren) plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed hyphae in the base are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on a nutrient-rich PDA plate, and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select a plate with no missing corners, well-developed hyphae in the base, and consistent growth for preservation. Other flat panels are eliminated, and the elimination rate is over 80%. Connect it to a PDA test tube and culture it for 10 days in a constant temperature incubator at 20°C-25°C. Take the front end, transfer it to the PDA test tube, and select the one with the best performance as the mother species for preservation.

technical field

[0001] The invention relates to a domestication method of the mother species of shimeji mushroom. Background technique

[0002] True jiji mushroom is a nutritious edible and medicinal rare edible fungus. The hot water and organic solvent extracts of its fruiting bodies have the effects of eliminating free radicals in the body, improving human immunity, and preventing aging. At present, the commercial cultivation of Shiji mushroom is one of the important varieties of industrial production of edible fungi. However, due to the long service life of the strains, the strains are prone to aging, and the aged mycelia have fewer growth points and weaker vigor, so the produced shimeji mushrooms will have weak anti-associated bacteria, poor stability, reduced growth vigor, and poor quality. Poor and yield reduction and so on. Strain domestication refers to the method of gradually adapting edible fungi to a certain condition through artificial measures, and the method ...

Examples

Embodiment 1

[0014] Select a relatively good mother species test tube for activation, the period is 3 days, connect it to about 15 water agar plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed silk are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on PDA plates and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select plates with no missing corners, well-developed hyphae in the base, and consistent growth for preservation, and eliminate other plates. The elimination rate is over 80%. Connect it to a PDA test tube (barren), and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. Select a test tube with no missing corners, well-developed hyphae in the base, and consistent growth, take the front end, and then transfer it to a PDA test tube, and select the o...

Embodiment 2

[0016] Select a relatively good mother seed test tube for activation, the period is 2 days, connect it on about 12 water agar (barren) plates, and cultivate it for 10 days under the condition of 20°C-25°C in a constant temperature incubator. The plates with more developed hyphae in the base are preserved, and the other plates are eliminated, with an elimination rate of more than 80%. Inoculate the screened strains on a nutrient-rich PDA plate, and culture them in a constant temperature incubator at 20°C-25°C for 10 days, then select a plate with no missing corners, well-developed hyphae in the base, and consistent growth for preservation. Other flat panels are eliminated, and the elimination rate is over 80%. Connect it to a PDA test tube and culture it for 10 days in a constant temperature incubator at 20°C-25°C. Take the front end, transfer it to the PDA test tube, and select the one with the best performance as the mother species for preservation.