A kind of vaccine composition and its preparation method and application

Abstract

The invention provides a vaccine composition, wherein, the vaccine composition comprises immunizing amount of chicken Newcastle disease virus antigen, chicken infectious bronchitis virus antigen and chicken mycoplasma synovial bursa antigen and carrier. The invention also provides the preparation method and application of the vaccine composition. The immune effect of the triple vaccine of the present invention is better than the immune effect of the combined use of the double inactivated vaccine of the new branch and the inactivated single vaccine of the mycoplasma synovial bursa. The present invention further provides a vaccine composition, which includes Mycoplasma gallisepticum antigen in addition to the above three antigens, and the vaccine composition can effectively resist the attack of four pathogens.

Description

technical field

[0001] The invention relates to a vaccine composition, a preparation method and application thereof, and belongs to the field of bioengineering. Background technique

[0002] Newcastle disease (New Castle disease) is a highly contagious and fatal disease caused by paramyxovirus, also known as Asian chicken plague or pseudo chicken plague, often showing symptoms of acute sepsis. The main features are dyspnea, loose stools, nervous disorders, mucosal and serosal hemorrhage, high mortality, and serious harm to the chicken industry. There is no effective treatment yet, so we can only rely on strict disinfection, isolation and vaccination with inactivated vaccines and live vaccines.

[0003] Avian infectious bronchitis disease is an acute and highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus. Chickens of all ages are susceptible, and its clinical characteristics are dyspnea, rales, coughing, mouth breathing, and s...

Examples

Embodiment 1

[0038] Example 1 The preparation of chicken Newcastle disease, infectious bronchitis virus liquid and mycoplasma synoviae bursae bacterial liquid

[0039] 1. Selection of virus strains

[0040] The LaSota strain of the Newcastle disease virus and the M41 strain of the infectious bronchitis virus were used to manufacture this product, both of which were purchased from the China Veterinary Drug Control Institute. Mycoplasma synovia was isolated and identified by the laboratory itself, and it was HN1 strain.

[0041] 2. Virus Fluid Preparation

[0042] 2.1 Preparation of virus solution for chicken Newcastle disease virus La Sota strain vaccine

[0043] 2.1.1 Inoculation

[0044] Take the virus seeds for production and dilute to 10 with sterile physiological saline -3 , Inoculate 10-day-old susceptible chicken embryos through the allantoic cavity, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs.

[0045] 2.1.2 ...

Embodiment 2

[0079] The preparation of embodiment 2 vaccine

[0080] 1 oil phase preparation

[0081] Take 94 parts of white oil for injection, add Siben-806 parts after mixing, add 2 parts of aluminum stearate, add and stir until transparent, autoclave for later use, which is the oil phase.

[0082] 2 Aqueous phase preparation

[0083] Mix the antigens in a sterile container in proportion, add 4% sterilized and cooled Tween-80, and stir while adding, until the Tween-80 is completely dissolved, which is the water phase.

[0084] 3 emulsification

[0085] Take 3 parts of the oil phase and put them in the oil phase tank, start the motor to stir, then slowly add 1 part of the water phase, transfer to the emulsification tank after adding, and then emulsify at 2800r / min for 40 minutes. Add 1% thimerosal before the emulsification is terminated to make the final concentration 0.01%. The specific formula of the vaccine is shown in the table below.

[0086] Table 1 Vaccine composition and cont...

Embodiment 3

[0116] Embodiment 3 Newcastle disease, infectious bronchitis, mycoplasma synovialis triple inactivated vaccine and Newcastle disease, infectious bronchitis double inactivated vaccine+mycoplasma synovialis inactivated vaccine immune side reaction comparison

[0117] Grouping and immunization of 1SPF chickens

[0118] Get 20 SPF chickens of age of 14 days, wherein 10 vaccine 1, 0.3ml / of subcutaneous immunization embodiment 2 preparations, other 10 vaccine 3 (0.3ml / ) and vaccine 4 (0.3ml / ) of subcutaneous immunization embodiment 2 preparations simultaneously 0.3ml / piece). In addition, 10 SPF chickens of the same age were not immunized as controls.

[0119] 2 side effects comparison

[0120] After 14 days of immunization, all chickens were weighed, and the body weight results are shown in Table 6. The results showed that, compared with the control group, the triple vaccine immunization group had no significant difference in body weight at 14 days after injection (ANOVA, P>0.05)...

technical field

[0001] The invention relates to a vaccine composition, a preparation method and application thereof, and belongs to the field of bioengineering. Background technique

[0002] Newcastle disease (New Castle disease) is a highly contagious and fatal disease caused by paramyxovirus, also known as Asian chicken plague or pseudo chicken plague, often showing symptoms of acute sepsis. The main features are dyspnea, loose stools, nervous disorders, mucosal and serosal hemorrhage, high mortality, and serious harm to the chicken industry. There is no effective treatment yet, so we can only rely on strict disinfection, isolation and vaccination with inactivated vaccines and live vaccines.

[0003] Avian infectious bronchitis disease is an acute and highly contagious respiratory infectious disease of chickens caused by infectious bronchitis virus. Chickens of all ages are susceptible, and its clinical characteristics are dyspnea, rales, coughing, mouth breathing, and s...

Examples

Embodiment 1

[0038] Example 1 The preparation of chicken Newcastle disease, infectious bronchitis virus liquid and mycoplasma synoviae bursae bacterial liquid

[0039] 1. Selection of virus strains

[0040] The LaSota strain of the Newcastle disease virus and the M41 strain of the infectious bronchitis virus were used to manufacture this product, both of which were purchased from the China Veterinary Drug Control Institute. Mycoplasma synovia was isolated and identified by the laboratory itself, and it was HN1 strain.

[0041] 2. Virus Fluid Preparation

[0042] 2.1 Preparation of virus solution for chicken Newcastle disease virus La Sota strain vaccine

[0043] 2.1.1 Inoculation

[0044] Take the virus seeds for production and dilute to 10 with sterile physiological saline -3 , Inoculate 10-day-old susceptible chicken embryos through the allantoic cavity, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs.

[0045] 2.1.2 ...

Embodiment 2

[0079] The preparation of embodiment 2 vaccine

[0080] 1 oil phase preparation

[0081] Take 94 parts of white oil for injection, add Siben-806 parts after mixing, add 2 parts of aluminum stearate, add and stir until transparent, autoclave for later use, which is the oil phase.

[0082] 2 Aqueous phase preparation

[0083] Mix the antigens in a sterile container in proportion, add 4% sterilized and cooled Tween-80, and stir while adding, until the Tween-80 is completely dissolved, which is the water phase.

[0084] 3 emulsification

[0085] Take 3 parts of the oil phase and put them in the oil phase tank, start the motor to stir, then slowly add 1 part of the water phase, transfer to the emulsification tank after adding, and then emulsify at 2800r / min for 40 minutes. Add 1% thimerosal before the emulsification is terminated to make the final concentration 0.01%. The specific formula of the vaccine is shown in the table below.

[0086] Table 1 Vaccine composition and cont...

Embodiment 3

[0116] Embodiment 3 Newcastle disease, infectious bronchitis, mycoplasma synovialis triple inactivated vaccine and Newcastle disease, infectious bronchitis double inactivated vaccine+mycoplasma synovialis inactivated vaccine immune side reaction comparison

[0117] Grouping and immunization of 1SPF chickens

[0118] Get 20 SPF chickens of age of 14 days, wherein 10 vaccine 1, 0.3ml / of subcutaneous immunization embodiment 2 preparations, other 10 vaccine 3 (0.3ml / ) and vaccine 4 (0.3ml / ) of subcutaneous immunization embodiment 2 preparations simultaneously 0.3ml / piece). In addition, 10 SPF chickens of the same age were not immunized as controls.

[0119] 2 side effects comparison

[0120] After 14 days of immunization, all chickens were weighed, and the body weight results are shown in Table 6. The results showed that, compared with the control group, the triple vaccine immunization group had no significant difference in body weight at 14 days after injection (ANOVA, P>0.05)...

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