Method for preparing hydroxyapatite and bone collagen composite coating on surface of magnesium alloy

Abstract

The invention relates to a method for preparing a hydroxyapatite and bone collagen composite coating on the surface of a magnesium alloy. The method comprises the following steps: preparing a deposition solution, wherein in the deposition solution, the concentration of calcium nitrate is 0.02-0.05 mol / L, the concentration of sodium phosphate is 0.002-0.005 mol / L, the concentration of bone collagen is 0.5-2.0 g / L, and the pH value of the deposition solution is 3.0-4.0; carrying out ultrasonic cleaning on the magnesium alloy for 8-10 min in alcohol and acetone after polishing treatment, and then putting the magnesium alloy in the deposition solution for deposition for 6-24 h, wherein ultrasonic oscillation with the power of 100-150 W and the frequency of 30-45 KHz is adopted in the initial stage of deposition for 5-15 min. The preparation method is simple, the prepared and obtained hydroxyapatite and bone collagen composite coating is low in degradation velocity, the biomedical magnesium alloy with the coating is higher in corrosion resistance, and the bone collagen is added into the deposition solution, so that the nucleation and growth of the hydroxyapatite can be quickened, and the corrosion resistance of materials is improved.

Description

technical field

[0001] The invention relates to a method for preparing a composite coating of hydroxyapatite and bone collagen on the surface of a magnesium alloy, and belongs to the technical field of preparation of biomedical materials. Background technique

[0002] As a metal material that can be degraded and absorbed, biomedical magnesium alloy can be completely absorbed by the human body when it is used for the repair of human hard tissues, avoiding the pain caused by the second removal operation. However, a key problem limiting the application of magnesium alloys is that the corrosion resistance of magnesium alloys is low, and their degradation speed is too fast, especially in solutions containing chloride ions. Therefore, calcium phosphate coating should be prepared on the surface of biomedical magnesium alloy. At present, the surface coating of magnesium alloy is mostly a single coating, and the most widely used is apatite coating, but the corrosion resistance of th...

Examples

Embodiment 1

[0023] First, add 0.95g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.16g of sodium phosphate to obtain a turbid solution, then add nitric acid with a concentration of 65-68% and stir to clarify the turbid solution, then add 0.1g Add distilled water, and dropwise add a nitric acid solution with a concentration of 65-68% and a sodium hydroxide solution with a concentration of 1mol / L to adjust the pH value to 4.0, adjust the total volume of the solution to 200mL, and obtain a solution containing 0.02mol / L The deposition solution of L calcium nitrate, 0.002mol / L sodium phosphate and 0.5g / L bone collagen;

[0024] The biomedical magnesium alloy sheet was first polished with sandpaper and then ultrasonically cleaned with alcohol and acetone for 8 minutes each. The magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 24 hours of deposition. In the initial stage of deposition, the...

Embodiment 2

[0028] First, add 2.36g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.39g of sodium phosphate to obtain a cloudy solution, then add nitric acid with a concentration of 65-68% and stir to clarify the cloudy solution, add 0.2 g of bone collagen, add distilled water, and use a concentration of 65-68% nitric acid and a concentration of 1mol / L sodium hydroxide to adjust the pH value to 3.0, adjust the total volume of the solution to 200mL, and obtain a solution containing 0.05mol / L calcium nitrate, 0.005 Deposition solution of mol / L sodium phosphate and 1.0g / L bone collagen;

[0029] The biomedical magnesium alloy sheet was first polished with sandpaper, then ultrasonically cleaned with alcohol and acetone for 10 minutes each, and then the magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 8 hours of deposition. In the initial stage of deposition, the power was 150W. Ultrasonic v...

Embodiment 3

[0033] First, add 1.43g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.19g of sodium phosphate to obtain a cloudy solution, then add nitric acid with a concentration of 65-68% and stir to clarify the cloudy solution, add 0.2 g of collagen, add distilled water, and be 65-68% nitric acid and 1mol / L sodium hydroxide to adjust the pH value to 4.0 with a concentration of 65-68%, adjust the total volume of the solution to 200mL, and obtain 0.03mol / L calcium nitrate 0.0025mol / L sodium phosphate and 1.0g / L collagen deposition solution;

[0034] The biomedical magnesium alloy sheet was first polished with sandpaper and ultrasonically cleaned with alcohol and acetone for 10 minutes each, and then the magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 24 hours of deposition. In the initial stage of deposition, the power was 100W, and the frequency 40KHz ultrasonic vibration for 10min; ...

technical field

[0001] The invention relates to a method for preparing a composite coating of hydroxyapatite and bone collagen on the surface of a magnesium alloy, and belongs to the technical field of preparation of biomedical materials. Background technique

[0002] As a metal material that can be degraded and absorbed, biomedical magnesium alloy can be completely absorbed by the human body when it is used for the repair of human hard tissues, avoiding the pain caused by the second removal operation. However, a key problem limiting the application of magnesium alloys is that the corrosion resistance of magnesium alloys is low, and their degradation speed is too fast, especially in solutions containing chloride ions. Therefore, calcium phosphate coating should be prepared on the surface of biomedical magnesium alloy. At present, the surface coating of magnesium alloy is mostly a single coating, and the most widely used is apatite coating, but the corrosion resistance of th...

Examples

Embodiment 1

[0023] First, add 0.95g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.16g of sodium phosphate to obtain a turbid solution, then add nitric acid with a concentration of 65-68% and stir to clarify the turbid solution, then add 0.1g Add distilled water, and dropwise add a nitric acid solution with a concentration of 65-68% and a sodium hydroxide solution with a concentration of 1mol / L to adjust the pH value to 4.0, adjust the total volume of the solution to 200mL, and obtain a solution containing 0.02mol / L The deposition solution of L calcium nitrate, 0.002mol / L sodium phosphate and 0.5g / L bone collagen;

[0024] The biomedical magnesium alloy sheet was first polished with sandpaper and then ultrasonically cleaned with alcohol and acetone for 8 minutes each. The magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 24 hours of deposition. In the initial stage of deposition, the...

Embodiment 2

[0028] First, add 2.36g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.39g of sodium phosphate to obtain a cloudy solution, then add nitric acid with a concentration of 65-68% and stir to clarify the cloudy solution, add 0.2 g of bone collagen, add distilled water, and use a concentration of 65-68% nitric acid and a concentration of 1mol / L sodium hydroxide to adjust the pH value to 3.0, adjust the total volume of the solution to 200mL, and obtain a solution containing 0.05mol / L calcium nitrate, 0.005 Deposition solution of mol / L sodium phosphate and 1.0g / L bone collagen;

[0029] The biomedical magnesium alloy sheet was first polished with sandpaper, then ultrasonically cleaned with alcohol and acetone for 10 minutes each, and then the magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 8 hours of deposition. In the initial stage of deposition, the power was 150W. Ultrasonic v...

Embodiment 3

[0033] First, add 1.43g of calcium nitrate tetrahydrate into 180mL of distilled water, stir to dissolve it completely, then add 0.19g of sodium phosphate to obtain a cloudy solution, then add nitric acid with a concentration of 65-68% and stir to clarify the cloudy solution, add 0.2 g of collagen, add distilled water, and be 65-68% nitric acid and 1mol / L sodium hydroxide to adjust the pH value to 4.0 with a concentration of 65-68%, adjust the total volume of the solution to 200mL, and obtain 0.03mol / L calcium nitrate 0.0025mol / L sodium phosphate and 1.0g / L collagen deposition solution;

[0034] The biomedical magnesium alloy sheet was first polished with sandpaper and ultrasonically cleaned with alcohol and acetone for 10 minutes each, and then the magnesium alloy sheet was immersed in a deposition solution heated to 40°C in a water bath for 24 hours of deposition. In the initial stage of deposition, the power was 100W, and the frequency 40KHz ultrasonic vibration for 10min; ...

Images