Bacillus licheniformis with high production of antimicrobial peptides and its application

Abstract

The invention discloses a Bacillus licheniformis, which is Bacillus?licheniformis ZJU-X1, which is preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC? No. 9688. The invention also discloses the bacillus licheniformis preparation prepared by using the bacillus licheniformis at the same time, and the bacillus licheniformis preparation is used as a feed additive. The present invention also simultaneously provides the animal microbial preparation prepared by utilizing the above-mentioned Bacillus licheniformis, which is used as a substitute for antibiotics in the feed of weaned piglets; in the animal microbial preparation, the viable count of the Bacillus licheniformis preparation is 1011 cfu / g.

Description

technical field

[0001] The invention relates to the field of feed additives---microbial preparations, in particular to a highly efficient and safe bacillus licheniformis and its application. Background technique

[0002] Feed antibiotics have significant effects in promoting animal growth, improving feed conversion efficiency, reducing food loss, and increasing pork production. However, with the widespread use of antibiotics as animal growth-promoting agents in feed, its disadvantages are becoming increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., causing a series of public safety and environmental problems. Conservation and livestock product quality issues. It has become one of the hotspots in the field of animal nutrition to search for green and safe feed additives that are non-toxic, efficient, and do not produce drug resistance.

[0003] Bacillus licheniformins (bacillus licheniformins) is one of th...

Examples

Embodiment 1

[0036] Isolation, identification and characteristic research of embodiment 1 bacterial classification

[0037] 1. Experimental method

[0038] 1.1 Experimental materials 10 copies of intestinal contents of healthy piglets from a pig farm in Zhejiang Province.

[0039] 1.2 Medium

[0040] ① Counting medium (both mass concentration g / L): peptone 10g, beef extract 5g, sodium chloride 5.0g, agar 15.0g, distilled water to 1.0L, pH 7.2, sterilized at 121°C for 15min.

[0041] ②LB medium (both mass concentration g / L): peptone 20g, K 2 HPO 4 .3H 2 O2.5g, MgSO 4 0.73g, 15.0ml glycerin, 15.0g agar, distilled water to 1.0L; pH 7.0, sterilized at 121°C for 15min.

[0042] 1.3 Specific experimental methods

[0043] 1) Take feces samples and add 10g of intestinal contents of healthy piglets to 90mL PBS buffer solution, put in 80°C water bath for 10min; thereby kill non-spore bacteria, then put into 20ml LB liquid medium, and incubate at 37°C for 24h;

[0044] 2) The samples were dil...

Embodiment 2

[0072] Example 2 Application of Bacillus licheniformis instead of antibiotics in weaned piglets

[0073] 1. Purpose and significance: Select weaned piglets as the test object, and investigate the effect of Bacillus licheniformis on the growth performance and diarrhea rate of weaned piglets without antibiotics.

[0074] 2. Materials and methods:

[0075] Experimental animals: Select 60 healthy weaned piglets of 23±2 days old Du×Long×Da Sanyuan crossbreeding, with a body weight of 7.8±0.2Kg.

[0076] Bacillus licheniformis preparation (animal microbial preparation): obtained by drying Bacillus licheniformis ZJU-X1 after liquid fermentation. Corn flour is used as a carrier. The number of viable bacteria in the prepared Bacillus licheniformis preparation is 10 11 cfu / g, in this implementation case, the Bacillus licheniformis preparation is used as an animal additive.

[0077] Remarks: The process steps and parameters of the above-mentioned Bacillus licheniformis ZJU-X1 liquid fe...

technical field

[0001] The invention relates to the field of feed additives---microbial preparations, in particular to a highly efficient and safe bacillus licheniformis and its application. Background technique

[0002] Feed antibiotics have significant effects in promoting animal growth, improving feed conversion efficiency, reducing food loss, and increasing pork production. However, with the widespread use of antibiotics as animal growth-promoting agents in feed, its disadvantages are becoming increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., causing a series of public safety and environmental problems. Conservation and livestock product quality issues. It has become one of the hotspots in the field of animal nutrition to search for green and safe feed additives that are non-toxic, efficient, and do not produce drug resistance.

[0003] Bacillus licheniformins (bacillus licheniformins) is one of th...

Examples

Embodiment 1

[0036] Isolation, identification and characteristic research of embodiment 1 bacterial classification

[0037] 1. Experimental method

[0038] 1.1 Experimental materials 10 copies of intestinal contents of healthy piglets from a pig farm in Zhejiang Province.

[0039] 1.2 Medium

[0040] ① Counting medium (both mass concentration g / L): peptone 10g, beef extract 5g, sodium chloride 5.0g, agar 15.0g, distilled water to 1.0L, pH 7.2, sterilized at 121°C for 15min.

[0041] ②LB medium (both mass concentration g / L): peptone 20g, K 2 HPO 4 .3H 2 O2.5g, MgSO 4 0.73g, 15.0ml glycerin, 15.0g agar, distilled water to 1.0L; pH 7.0, sterilized at 121°C for 15min.

[0042] 1.3 Specific experimental methods

[0043] 1) Take feces samples and add 10g of intestinal contents of healthy piglets to 90mL PBS buffer solution, put in 80°C water bath for 10min; thereby kill non-spore bacteria, then put into 20ml LB liquid medium, and incubate at 37°C for 24h;

[0044] 2) The samples were dil...

Embodiment 2

[0072] Example 2 Application of Bacillus licheniformis instead of antibiotics in weaned piglets

[0073] 1. Purpose and significance: Select weaned piglets as the test object, and investigate the effect of Bacillus licheniformis on the growth performance and diarrhea rate of weaned piglets without antibiotics.

[0074] 2. Materials and methods:

[0075] Experimental animals: Select 60 healthy weaned piglets of 23±2 days old Du×Long×Da Sanyuan crossbreeding, with a body weight of 7.8±0.2Kg.

[0076] Bacillus licheniformis preparation (animal microbial preparation): obtained by drying Bacillus licheniformis ZJU-X1 after liquid fermentation. Corn flour is used as a carrier. The number of viable bacteria in the prepared Bacillus licheniformis preparation is 10 11 cfu / g, in this implementation case, the Bacillus licheniformis preparation is used as an animal additive.

[0077] Remarks: The process steps and parameters of the above-mentioned Bacillus licheniformis ZJU-X1 liquid fe...

Images