Scar-removal composition and dressing

Abstract

The invention provides a scar-removal composition, which comprises a fibroblast culture solution, a fibroblast growth factor and an aloe extract. Mass concentration of the fibroblast growth factor is 10-50 ng / mL. Mass percentage of the aloe extract is 0.1-1%. The fibroblast culture solution is obtained after fibroblast at passage P1-P5 is successively cultured with a mixed culture medium and a phenol red-free culture medium. The mixed culture medium contains a DMEM culture medium and fetal calf serum. The aloe extract is prepared by the following method: separating epidermis of fresh Aloe barbadensis leaf and mesophyll, squeezing gel juice from mesophyll, and successively degerming, concentrating and freeze-drying the gel juice so as to obtain an aloe extract. The fibroblast culture solution is safe and effective. With the fibroblast culture solution cooperating with the fibroblast growth factor and the aloe extract, the product can promote skin regeneration and reduce inflammation and formation of scar tissues. The invention also provides a dressing.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a scar-removing composition and a dressing. Background technique

[0002] The acne scars left by puberty leave an indelible "mark of youth". With the progress of modern society, love of beauty not only represents a kind of right, but also a kind of respect for self. How to dilute this unpleasant "imprint of youth" has become a topic of concern to many beauty lovers.

[0003] Scar removal can be achieved through drugs, laser or surgery. Surgical laser methods have certain risks. Most of the drug scar removal products on the market currently use some organic macromolecular chemical products containing benzene rings or add some hormones to enhance the scar removal effect. Scar effect, such as the Chinese patent whose publication number is CN105055235A, has adopted chemical substances such as simethicone, paraben and triethanolamine, which inevitably has certain dama...

Examples

Embodiment 1

[0068] 1. Collection of fibroblast conditioned medium

[0069] Take the P3 fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.15ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 300 g for 5 minutes. The supernatant was taken and passed through a 0.22 μm filter membrane to obtain the fibroblast conditioned medium for future use.

[0070] 3D printed dressing

[0071] 2. 3D modeling

[0072] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image into a stereolithography ...

Embodiment 2

[0096] 1. Collection of Fibroblast-Conditioned Medium

[0097] Take P3 generation fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.10ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 200 g for 5 minutes, and the supernatant was taken and passed through a 0.22 μm filter membrane to obtain the fibroblast conditioned medium for use.

[0098] 3D printed dressing

[0099] 2.3D modeling

[0100] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image into a stereolithograp...

Embodiment 3

[0109] 1. Collection of Fibroblast-Conditioned Medium

[0110] Take P3 generation fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.2ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 400 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the obtained conditioned medium was fibroblast conditioned medium for use.

[0111] 3D printed dressing

[0112] 2.3D modeling

[0113] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image...

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a scar-removing composition and a dressing. Background technique

[0002] The acne scars left by puberty leave an indelible "mark of youth". With the progress of modern society, love of beauty not only represents a kind of right, but also a kind of respect for self. How to dilute this unpleasant "imprint of youth" has become a topic of concern to many beauty lovers.

[0003] Scar removal can be achieved through drugs, laser or surgery. Surgical laser methods have certain risks. Most of the drug scar removal products on the market currently use some organic macromolecular chemical products containing benzene rings or add some hormones to enhance the scar removal effect. Scar effect, such as the Chinese patent whose publication number is CN105055235A, has adopted chemical substances such as simethicone, paraben and triethanolamine, which inevitably has certain dama...

Examples

Embodiment 1

[0068] 1. Collection of fibroblast conditioned medium

[0069] Take the P3 fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.15ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 300 g for 5 minutes. The supernatant was taken and passed through a 0.22 μm filter membrane to obtain the fibroblast conditioned medium for future use.

[0070] 3D printed dressing

[0071] 2. 3D modeling

[0072] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image into a stereolithography ...

Embodiment 2

[0096] 1. Collection of Fibroblast-Conditioned Medium

[0097] Take P3 generation fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.10ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 200 g for 5 minutes, and the supernatant was taken and passed through a 0.22 μm filter membrane to obtain the fibroblast conditioned medium for use.

[0098] 3D printed dressing

[0099] 2.3D modeling

[0100] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image into a stereolithograp...

Embodiment 3

[0109] 1. Collection of Fibroblast-Conditioned Medium

[0110] Take P3 generation fibroblasts and culture them with DMEM+10% FBS. When the cells are in the exponential growth phase and the confluence reaches 80%, remove the culture supernatant, wash twice with PBS, and add 0.2ml / cm 2 After the phenol red-free 1640 was cultured for 72 hours, the conditioned medium was collected, and the collected conditioned medium was centrifuged at 400 g for 5 minutes, and the supernatant was taken, passed through a 0.22 μm filter membrane, and the obtained conditioned medium was fibroblast conditioned medium for use.

[0111] 3D printed dressing

[0112] 2.3D modeling

[0113] Use the built-in laser of the 3D printer to scan the surface of the patient's scar to obtain data such as the size, shape, thickness of the wound, and the thickness of the epidermis and dermis of the patient's normal skin. Edit to make it produce a continuous surface image; use Studio4.0 software to convert the image...

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