Drug-loaded creased graphite pebble photo-thermal preparation, and preparation and application thereof

Abstract

The invention relates to a drug-loaded creased graphite pebble photo-thermal preparation, and preparation and application thereof. A preparation method for the drug-loaded creased graphite pebble photo-thermal preparation comprises the following steps: adding an aqueous solution of graphene oxide into an aqueous drug solution, adjusting a pH value, and carrying out shaking-up so as to obtain a drug-loaded compound; and preparing an aqueous solution of the drug-loaded compound, carrying out atomization, allowing atomized aerosol drops to pass through a quartz tube, carrying out collection with a polytetrafluoroethylene (PTFE) filter membrane at a tail end, and then carrying out drying so as to obtain the preparation. The drug-loaded compound and cancer cells are subjected to co-culture, infrared light is used for irradiation, and comparison with a non-illumination group and a pure DOX group is carried out so as to detect the killing effect of a material on cancer cells. The novel drug-loaded photo-thermal preparation in the invention has unique photo-thermal characteristics, is simple to prepare and suitable for industrial large-scale preparation, can be used for antineoplastic research and has good practical value.

Description

technical field

[0001] The invention belongs to the field of photothermal preparation and its preparation and application, in particular to a drug-loaded wrinkled graphite sphere photothermal preparation and its preparation and application. Background technique

[0002] Long-term consumption of toxic substances can easily lead to an increase in the incidence of cancer. The current treatment methods mainly include chemotherapy, surgery and radiotherapy. Chemotherapy is a treatment method that uses chemical drugs to prevent the proliferation, invasion, and metastasis of cancer cells until it finally kills cancer cells, but its selectivity is not strong, and it will inevitably damage normal human cells while killing cancer cells , so that patients have adverse reactions; surgical treatment can only be used for patients with early cancer cells that have not spread, and surgical treatment for patients whose tumor cells have metastasized usually cannot be cured, and it will cause...

Examples

Embodiment 1

[0029] The preparation of GO / DOX complex comprises the following steps:

[0030] Weighing 40ml of GO solution with a concentration of 1mg / ml and mixing it with 40ml of DOX solution with a concentration of 2.0mg / ml, so that GO:DOX=1:2, and then adjusting the pH of the mixed solution with a concentration of 0.1M sodium hydroxide solution value, making the pH of the mixture = 9. The adjusted mixture was placed on a shaker at a temperature of 37° C. and stirred for 4 hours to obtain a drug-loaded complex. Pour the solution into a centrifuge tube, centrifuge in a centrifuge at 10,000rmp for 3min, pour off the supernatant, and add 10ml of ultrapure water. Freeze-dry under dark conditions, wrap the complex in aluminum foil and store in a refrigerator at 4°C for later use.

Embodiment 2

[0032] The preparation of Crumpled-rGO / DOX comprises the following steps:

[0033] Accurately weigh 20 mg of the GO / DOX complex in Example (1) with a balance and dissolve it in 20 ml of ultrapure water to prepare a solution with a concentration of 1.0 mg / ml, and place the solution in an ultrasonic atomizer connected to a quartz tube Internally (physical and chemical oscillation frequency is 1.7MHz ~ 2.1MHz) to make it atomized into aerosol droplets, after passing through the quartz tube heated to 180°C under the drive of atomizing airflow and suction vacuum pump, use a PTFE filter membrane with a pore size of 0.22μm Collected, dried at room temperature for 3 hours, and then placed in a vacuum desiccator at 60°C for 24 hours to obtain Crumpled-rGO / DOX.

Embodiment 3

[0035] Accurately prepare 10mL of Crumpled-rGO (Example 2) aqueous solution with a concentration of 200μg / mL, and irradiate with lasers of different intensities. The laser intensities are: 0.5, 1, 1.5, 2W / cm 2 . Using a laser with a wavelength of 808nm to irradiate for 5 minutes, the temperature change of the solution is recorded by an infrared thermal imager, and finally the heating curve of the solution under the laser irradiation and the real-time video of the solution can be obtained, see figure 1 and figure 2 .

[0036] In order to study the photothermal stability of the material, the concentration of the material aqueous solution is 200μg / mL when the laser intensity is 1W / cm 2 , the irradiation time is 5min, and the heating and cooling cycle curve under the condition of 5 consecutive on-off irradiations is measured to further study the photothermal stability of the material. The stability curve is shown in image 3 .

[0037] Photothermal properties of crumpled gra...

technical field

[0001] The invention belongs to the field of photothermal preparation and its preparation and application, in particular to a drug-loaded wrinkled graphite sphere photothermal preparation and its preparation and application. Background technique

[0002] Long-term consumption of toxic substances can easily lead to an increase in the incidence of cancer. The current treatment methods mainly include chemotherapy, surgery and radiotherapy. Chemotherapy is a treatment method that uses chemical drugs to prevent the proliferation, invasion, and metastasis of cancer cells until it finally kills cancer cells, but its selectivity is not strong, and it will inevitably damage normal human cells while killing cancer cells , so that patients have adverse reactions; surgical treatment can only be used for patients with early cancer cells that have not spread, and surgical treatment for patients whose tumor cells have metastasized usually cannot be cured, and it will cause...

Examples

Embodiment 1

[0029] The preparation of GO / DOX complex comprises the following steps:

[0030] Weighing 40ml of GO solution with a concentration of 1mg / ml and mixing it with 40ml of DOX solution with a concentration of 2.0mg / ml, so that GO:DOX=1:2, and then adjusting the pH of the mixed solution with a concentration of 0.1M sodium hydroxide solution value, making the pH of the mixture = 9. The adjusted mixture was placed on a shaker at a temperature of 37° C. and stirred for 4 hours to obtain a drug-loaded complex. Pour the solution into a centrifuge tube, centrifuge in a centrifuge at 10,000rmp for 3min, pour off the supernatant, and add 10ml of ultrapure water. Freeze-dry under dark conditions, wrap the complex in aluminum foil and store in a refrigerator at 4°C for later use.

Embodiment 2

[0032] The preparation of Crumpled-rGO / DOX comprises the following steps:

[0033] Accurately weigh 20 mg of the GO / DOX complex in Example (1) with a balance and dissolve it in 20 ml of ultrapure water to prepare a solution with a concentration of 1.0 mg / ml, and place the solution in an ultrasonic atomizer connected to a quartz tube Internally (physical and chemical oscillation frequency is 1.7MHz ~ 2.1MHz) to make it atomized into aerosol droplets, after passing through the quartz tube heated to 180°C under the drive of atomizing airflow and suction vacuum pump, use a PTFE filter membrane with a pore size of 0.22μm Collected, dried at room temperature for 3 hours, and then placed in a vacuum desiccator at 60°C for 24 hours to obtain Crumpled-rGO / DOX.

Embodiment 3

[0035] Accurately prepare 10mL of Crumpled-rGO (Example 2) aqueous solution with a concentration of 200μg / mL, and irradiate with lasers of different intensities. The laser intensities are: 0.5, 1, 1.5, 2W / cm 2 . Using a laser with a wavelength of 808nm to irradiate for 5 minutes, the temperature change of the solution is recorded by an infrared thermal imager, and finally the heating curve of the solution under the laser irradiation and the real-time video of the solution can be obtained, see figure 1 and figure 2 .

[0036] In order to study the photothermal stability of the material, the concentration of the material aqueous solution is 200μg / mL when the laser intensity is 1W / cm 2 , the irradiation time is 5min, and the heating and cooling cycle curve under the condition of 5 consecutive on-off irradiations is measured to further study the photothermal stability of the material. The stability curve is shown in image 3 .

[0037] Photothermal properties of crumpled gra...

Images