Breeding method of high-linoleic acid-content black soybean

Abstract

The invention discloses a method for breeding black soybeans with high linoleic acid content, which is characterized in that it comprises the following aspects: (1) radiation mutagenesis, the black soybeans are sterilized first, and then immersed in a fermented nutrient solution for irradiation Mutagenesis; (2) greenhouse selection, irradiated seeds were cultured in the greenhouse, and seeds with relatively high linoleic acid content were screened out; (3) secondary mutagenesis, under high carbon dioxide concentration conditions, use 320-350lux respectively and 440‑470lux ultraviolet light intensity for secondary mutagenesis; (4) secondary seed selection, greenhouse cultivation of secondary mutagenesis seeds, to obtain 1st generation seeds to screen high linoleic acid content seeds, and greenhouse cultivation to obtain 2nd generation seeds (5) Hybridization, hybridization of black soybeans and 1st generation seeds to obtain 1st generation of hybridization, and then repeated 2 times of hybridization of female parent hybridization with 1st generation and 2nd generation seeds to obtain 3rd generation of hybridization for large-scale planting.

Description

technical field

[0001] The invention belongs to the technical field of black soybean breeding, in particular to a method for breeding black soybeans with high linoleic acid content. Background technique

[0002] Black soybeans, also known as scull beans and black beans, are the seeds of leguminous plants. They have the characteristics of high protein and low calorie. The outer skin is black, and the inside is yellow or green. 30°C, black soybeans are mainly distributed in Heilongjiang, Liaoning, Jilin, Anhui, Henan, Hebei, Shandong, Jiangsu and other places in China. It has strong adaptability to land, temperature and other environments and has a wide range of planting. Black soybeans are rich in nutrients such as protein, ash, vitamins and isoflavones, and also contain saponins, black soybean polysaccharides and black soybean pigments, which have the effects of lowering cholesterol, strengthening the spleen and kidney, eliminating phlegm and relieving cough, anti-aging and ...

Examples

Embodiment 1

[0019] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:

[0020] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The γ-ray irradiation intensity is 73 rem for 15min / h, the time is 7.5h, and the γ-ray irradiation intensity is 115 rem for 12min / h, the time is 12.5h, and the irradiated seeds are obtained; the seed germination stage is relatively fragile, use Low irradiance, strong vitality after leaf bud development Using relatively high ultraviolet light can increase the probability of mutation;

[0021] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 3:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds, spray the fermented nutrient...

Embodiment 2

[0032] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:

[0033] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The intensity of gamma ray irradiation is 85 rem for 17min / h for 8h, and then the intensity of gamma ray irradiation is 120 rem for 14min / h for 13h to obtain irradiated seeds; the germination stage of seeds is relatively fragile, and low radiation Illumination, the vitality of the leaf buds is strong after development, and the use of relatively high ultraviolet light can increase the probability of mutagenesis;

[0034] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 4:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds,...

technical field

[0001] The invention belongs to the technical field of black soybean breeding, in particular to a method for breeding black soybeans with high linoleic acid content. Background technique

[0002] Black soybeans, also known as scull beans and black beans, are the seeds of leguminous plants. They have the characteristics of high protein and low calorie. The outer skin is black, and the inside is yellow or green. 30°C, black soybeans are mainly distributed in Heilongjiang, Liaoning, Jilin, Anhui, Henan, Hebei, Shandong, Jiangsu and other places in China. It has strong adaptability to land, temperature and other environments and has a wide range of planting. Black soybeans are rich in nutrients such as protein, ash, vitamins and isoflavones, and also contain saponins, black soybean polysaccharides and black soybean pigments, which have the effects of lowering cholesterol, strengthening the spleen and kidney, eliminating phlegm and relieving cough, anti-aging and ...

Examples

Embodiment 1

[0019] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:

[0020] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The γ-ray irradiation intensity is 73 rem for 15min / h, the time is 7.5h, and the γ-ray irradiation intensity is 115 rem for 12min / h, the time is 12.5h, and the irradiated seeds are obtained; the seed germination stage is relatively fragile, use Low irradiance, strong vitality after leaf bud development Using relatively high ultraviolet light can increase the probability of mutation;

[0021] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 3:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds, spray the fermented nutrient...

Embodiment 2

[0032] A method for breeding black soybeans with high linoleic acid content, comprising the following steps:

[0033] (1) Irradiation mutagenesis: The black soybean seeds are sterilized, and then immersed in the fermentation nutrient solution for irradiation mutagenesis. The organic selenium produced after fermentation can improve the stress resistance of the seeds and reduce the radiation damage. The intensity of gamma ray irradiation is 85 rem for 17min / h for 8h, and then the intensity of gamma ray irradiation is 120 rem for 14min / h for 13h to obtain irradiated seeds; the germination stage of seeds is relatively fragile, and low radiation Illumination, the vitality of the leaf buds is strong after development, and the use of relatively high ultraviolet light can increase the probability of mutagenesis;

[0034] (2) Seed selection in the greenhouse: mix the loess and organic fertilizer at a ratio of 4:1, lay the bottom of the greenhouse planting bed, sow the irradiated seeds,...

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