A dynamic cell culture plate

Abstract

The present invention relates to the field of experimental instruments, and discloses a dynamic cell culture plate, which includes a support plate, a cell culture hole, a driving device, a support seat, a driving shaft, a gear, a connecting rod, a culture medium tank, a reserved hole and an impeller. When in use, the driving device is started, and the driving device drives the gear and the connecting rod to rotate through the driving shaft, and the impeller at the end of the connecting rod rotates accordingly. Under the action of the impeller, the culture medium will flow in the culture medium tank, so that the cells in the cell culture hole are in a dynamic environment, and dynamic cell culture is achieved. The dynamic cell culture plate of the present invention solves the problem that most existing cell culture plates are static culture plates and cannot simulate the dynamic environment in the body to dynamically culture cells. It has the advantages of small size, low cost, easy use, and can improve the accuracy and scientificity of experimental results.

Description

Technical Field

[0001] The invention relates to the field of experimental instruments, and more specifically to a dynamic cell culture plate. Background Art

[0002] At present, most of the cell culture plates commonly used in laboratories are static culture plates. The culture medium is static and the cells are cultured in a static environment. In the body, the tissue fluid can flow, so the cells live in a dynamic environment. The state and function of cells cultured on static cell culture plates are different from those cultured under dynamic conditions, and the experimental results may also be different from the cell detection results in the body. Therefore, it is necessary to use dynamic cell culture plates to culture cells in a dynamic environment, maintain normal cell function, differentiation, secretion, etc., and improve the accuracy and scientificity of the experimental results.

[0003] There is also an instrument on the market that can culture cells under flow conditions. However, it is mainly used to detect fluid shear force. It is a fluid shear force system. Its shortcomings are: 1. It is not suitable for various experiments and has a small scope of use; 2. The instrument is expensive, and its cell plate is also expensive and costly; 3. It requires many devices to be connected during use, which is inconvenient to use; 4. It is large in size and inconvenient to be placed in a cell culture incubator for use.

[0004] In summary, it is necessary to develop a dynamic cell culture plate that is small in size, low in cost, easy to use, and can simulate the dynamic environment in vivo to culture cells and improve the accuracy and scientificity of experimental results. Summary of the invention

[0005] In order to overcome at least one defect (shortcoming) of the above-mentioned prior art, the present invention provides a dynamic cell culture plate which is small in size, low in cost and easy to use.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A dynamic cell culture plate, comprising a support plate and a cell culture hole, characterized in that the dynamic cell culture plate also comprises a driving device, a support seat, a driving shaft, a gear, a connecting rod, a culture medium tank, a reserved hole and an impeller, wherein the driving device is fixedly connected to the support seat, the driving shaft is connected to the connecting rod through a gear, the driving device drives the gear and the connecting rod to rotate through the driving shaft, the connecting rod passes through the side wall of the culture medium tank and an impeller is arranged at the end of the connecting rod, and the cell culture hole and the reserved hole are arranged inside the culture medium tank. The dynamic cell culture plate of the present invention is small in size, low in cost, and easy to use. It simulates the dynamic environment in the body to culture cells, and can improve the accuracy and scientificity of experimental results.

[0008] Furthermore, the cell culture wells of the dynamic cell culture plate are symmetrically provided with through holes on the side walls, and each cell culture well is equipped with a detachable circular sleeve, the height of the sleeve is slightly higher than the cell culture well, and it is easy to take out from the cell culture well. The cell culture well is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve is first placed in the cell culture well, so that the through holes on the side walls of the cell culture well are blocked, and the culture medium in the cell culture well is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well. After the cells are completely attached, the sleeve is removed, so that the culture medium in the cell culture well and the culture medium in the culture medium tank can be merged into one.

[0009] Furthermore, the culture medium tanks of the dynamic cell culture plate are symmetrically arranged on the support plate in a square shape, distributed on both sides of the drive, the driving device is arranged on the support seat, the support seat is arranged on the support plate, one end of the driving shaft is connected to the driving device, and the other end of the driving shaft is movably connected to the support rod arranged on the support plate, and external threads are arranged at equal distances on the surface of the driving shaft, and the external threads are meshed with gears fixedly arranged in the middle of the connecting rod. The two ends of the connecting rod respectively penetrate the corresponding side walls of the culture medium tank and the ends are provided with impellers. The culture medium tanks are distributed in a square shape, which can adapt to more experimental occasions, and the number of culture medium tanks can be increased or decreased in even numbers according to the needs of the experiment. The gear transmission method is adopted, which can not only realize the change of direction of the driving force, but also has the characteristics of stable transmission, high efficiency and compact structure.

[0010] Furthermore, a cell culture hole and a reserved hole are provided in the culture medium tank with a square distribution. The cell culture hole is used to culture adherent cells, and the reserved hole can be used to form a special flow channel of the culture medium in the culture medium tank by utilizing its outer structure, and cells can also be statically cultured in the reserved hole, or used as a control observation area for cell culture.

[0011] Furthermore, two cell culture holes and one reserved hole are arranged in the culture medium tank with a square distribution. The purpose of setting the two cell culture holes is to culture two kinds of cells, and each cell culture hole cultures one kind of cell, so that the two kinds of cells live in the same flowing culture medium, and the interaction and influence between the two kinds of cells can be analyzed and observed. The reserved hole can be arranged to form a special flow channel of the culture medium in the culture medium tank by utilizing its outer shape structure, and cells can also be statically cultured in the reserved hole, or used as a control observation area for cell culture.

[0012] Furthermore, the culture medium tank of the dynamic cell culture plate is arranged on the support plate in a circular shape, the driving device is arranged inside the support seat, the support seat is arranged above the support plate through a supporting column, one end of the driving shaft passes through the bottom of the support seat to connect to the driving device, the other end of the driving shaft is provided with a first bevel gear, one end of the connecting rod is provided with a second bevel gear and meshes with the first bevel gear arranged at the end of the driving shaft, the other end of the connecting rod passes through the corresponding culture medium tank side wall and the end is provided with an impeller, and there is a cell culture hole and a reserved hole in the culture medium tank. The culture medium tank is distributed in a circular shape, which can adapt to more experimental occasions, and the number of culture medium tanks can be increased or decreased according to the needs of the experiment, and it only needs to increase or decrease the number of connecting rods with bevel gears at the same time. The gear transmission method is adopted, which can not only realize the change of direction of the driving force, but also has the characteristics of stable transmission, high efficiency and compact structure.

[0013] Furthermore, a rubber sealing ring is provided at the part where the connecting rod of the dynamic cell culture plate penetrates the side wall of the culture medium tank. In this way, during the rotation of the connecting rod, the wear at the joint between the side wall of the culture medium tank and the connecting rod can be reduced, noise can be eliminated, and the rubber sealing ring can be used to prevent the culture medium from leaking to the outside. At the same time, the rubber sealing ring can also be used as a fulcrum of the connecting rod to ensure the dynamic balance of the connecting rod.

[0014] Furthermore, the impeller of the dynamic cell culture plate can be an open impeller, a semi-open impeller or a closed impeller. The present invention preferably uses a closed impeller, which is composed of a front cover plate, a rear cover plate and an arc-shaped blade, and the arc-shaped blade is fixedly arranged between the front cover plate and the rear cover plate. The closed impeller has a higher efficiency and can prevent the culture solution from splashing around, ensuring that the culture medium flows smoothly under the action of the impeller.

[0015] Furthermore, the shapes of the culture medium tank, cell culture hole and reserved hole of the dynamic cell culture plate can be made into any shape as long as the technical effect of the present invention can be achieved and the requirements of dynamic cell culture can be met.

[0016] Furthermore, in order to prevent the cell sample from being contaminated, the dynamic cell culture plate can be equipped with a transparent cover, and the material of the transparent cover can be made of plastic, organic glass and other materials, which is convenient for observing the cell culture state and can isolate the cell sample from the outside world to prevent the cell sample from being contaminated. The shape of the transparent cover can be made into any shape, as long as it can isolate the cell sample on the cell culture plate from the external environment, and it will not be described in detail in the present invention.

[0017] In some embodiments, the driving device of the dynamic cell culture plate with the culture medium slots arranged in a square shape is a motor device, a battery is used to provide power for the motor, the bottom of the motor device is fixedly connected to the support base, one side of the motor device is connected to the driving shaft, a battery box and a power line are arranged inside the support base, the power line connects the motor and the battery box, and a power switch is arranged outside the support base. The motor device is driven, the structure is simple, the volume is small, the use is convenient, and the operation is long-lasting and stable.

[0018] In some embodiments, the driving device of the dynamic cell culture plate with the culture medium slots arranged in a square shape is a clockwork device, the bottom of the clockwork device is fixedly connected to the support seat, one side of the clockwork device is connected to the driving shaft, and the other side of the clockwork device is provided with a clockwork handle. The clockwork device is driven without energy consumption, has a simple structure, a small size, and is easy to use.

[0019] In some embodiments, the driving device of the dynamic cell culture plate with circular culture medium grooves is a motor device, a battery is used to provide power for the motor, the motor device is installed inside the support base, the driving shaft passes through the bottom of the support base and is connected to the motor device, a power switch is arranged outside the support base, a battery box and a power cord are also arranged inside the support base, and the power cord connects the motor and the battery box. The motor device is driven, the structure is simple, the volume is small, the use is convenient, and the operation is long-lasting and stable.

[0020] In some embodiments, the driving device of the dynamic cell culture plate with circular culture medium slots is a clockwork device, which is installed inside the support seat, the driving shaft passes through the bottom of the support seat and is connected to one side of the clockwork device, and the clockwork handle passes through the top of the support seat and is connected to the other side of the clockwork device. The clockwork device is driven without energy consumption, has a simple structure, a small size, and is easy to use.

[0021] Regardless of which implementation method is adopted, the motor device and the clockwork device are both conventional driving devices, so their structural principles are not described in detail.

[0022] When the dynamic cell culture plate disclosed in the present invention is in use, the driving device is started, and the driving device drives the gear arranged on the connecting rod to rotate through the driving shaft. The connecting rod and the impeller arranged at the end of the connecting rod rotate with the gear. Under the action of the impeller, the culture medium will flow in the culture medium groove, so that the cells in the cell holes are in a dynamic environment, thereby realizing dynamic cell culture.

[0023] The descriptions of “first” and “second” mentioned above are for descriptive purposes only and should not be understood as indicating or suggesting their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include at least one such feature.

[0024] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0025] 1. Small size: easy to put into cell culture box, clean bench and other instruments;

[0026] 2. Low cost: simple structure, few accessories, easy to manufacture;

[0027] 3. Easy to use: simple operation, no need to connect too many devices and AC power supply;

[0028] 4. Cultivating cells by simulating the dynamic environment in vivo can improve the accuracy and scientificity of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0030] Figure 1 is a schematic diagram of the three-dimensional structure of implementation mode 1;

[0031] Figure 2 is a top view of implementation mode 1;

[0032] Figure 3 is a schematic diagram of the three-dimensional structure of implementation mode 2;

[0033] Figure 4 is a top view of implementation mode 2;

[0034] Figure 5 is a schematic diagram of the three-dimensional structure of implementation mode 3;

[0035] Figure 6 is a top view of implementation mode 3;

[0036] Figure 7 is a schematic diagram of the three-dimensional structure of implementation mode 4;

[0037] Figure 8 is a top view of implementation mode 4;

[0038] Fig. 9 is a schematic diagram of the three-dimensional structure of implementation mode 5;

[0039] Fig.10 is a schematic diagram of the three-dimensional structure of implementation mode 6;

[0040] Fig.11 Schematic diagram of the connection structure of each component of implementation mode 5-6;

[0041] Fig.12 It is a schematic diagram of the structure of the cell culture hole and the sleeve;

[0042] Fig.13 Schematic diagram of the three-dimensional structure of the impeller;

[0043] Fig.14 for Fig.13 AA section view;

[0044] Fig.15 Schematic diagram of the connection structure between the drive shaft and the gear in implementation mode 1-4;

[0045] Fig.16 It is a side view of the first and second bevel gear connection structure of implementation mode 5-6.

[0046] In the figure: 1- support plate, 2- cell culture well, 3- driving device, 4- driving shaft, 5- supporting rod,

[0047] 6-support seat, 7-support column, 8-gear, 9-external thread, 10-connecting rod,

[0048] 11-first bevel gear, 12-second bevel gear, 13-culture medium tank, 14-impeller,

[0049] 15-reserved hole, 16-sleeve, 17-through hole, 18-rubber sealing ring, 19-spring handle,

[0050] 20-Power switch. DETAILED DESCRIPTION

[0051] The drawings are only used for illustrative purposes and should not be construed as limitations on this patent. In order to better illustrate this embodiment, some parts of the drawings may be omitted, enlarged, or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0052] The descriptions of “first” and “second” involved in the present invention are for descriptive purposes only and should not be understood as indicating or suggesting their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include at least one of such features.

[0053] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, which can be said to be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0054] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described in detail with reference to the accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0055] Implementation 1:

[0056] like Figure 1 , Figure 2 and Fig.15 As shown, a dynamic cell culture plate includes a support plate 1, a cell culture hole 2, a driving device 3, a support seat 6, a driving shaft 4, a gear 8, a connecting rod 10, a culture medium tank 13, a reserved hole 15 and an impeller 14. The driving device 3 is fixedly connected to the support seat 6, the driving shaft 4 is connected to the connecting rod 10 through the gear 8, the driving device 3 drives the gear 8 and the connecting rod 10 to rotate through the driving shaft 4, the connecting rod 10 passes through the side wall of the culture medium tank 13 and the impeller 14 is arranged at the end of the connecting rod 10, and the cell culture hole 2 and the reserved hole 15 are arranged inside the culture medium tank 13.

[0057] The culture medium tank 13 of the dynamic cell culture plate is symmetrically arranged on the support plate 1 in a square shape, distributed on both sides of the driving shaft 4, the driving device 3 is arranged on the support seat 6, and the support seat 6 is arranged on the support plate 1, one end of the driving shaft 4 is connected to the driving device 3, and the other end of the driving shaft 4 is movably connected to the support rod 5 arranged on the support plate 1, and the surface of the driving shaft 4 is equidistantly provided with external threads 9, and the external threads 9 are meshed with the gear 8 fixedly arranged in the middle of the connecting rod 10, and the two ends of the connecting rod 10 respectively penetrate the corresponding side walls of the culture medium tank 13 and the end is provided with an impeller 14. The culture medium tank 13 is distributed in a square shape, which can adapt to more experimental occasions, and the number of the culture medium tank 13 can be increased or decreased in even numbers according to the needs of the experiment.

[0058] A cell culture hole 2 and a reserved hole 15 are arranged in the culture medium tank 13 with a square distribution. The cell culture hole 2 is used to culture adherent cells, and the reserved hole 15 can be used to form a special flow channel of the culture medium in the culture medium tank 13 by utilizing its outer structure, and cells can also be statically cultured in the reserved hole 15, or used as a control observation area for cell culture.

[0059] The connecting rod 10 of the dynamic cell culture plate is provided with a rubber sealing ring 18 at the position where it penetrates the side wall of the culture medium tank 13. In this way, during the rotation of the connecting rod 10, it can reduce the wear of the joint between the side wall of the culture medium tank 13 and the connecting rod 10, eliminate noise, and play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 10 to ensure the dynamic balance of the connecting rod 10.

[0060] The driving device 3 of the dynamic cell culture plate is a motor device, which uses a battery to provide power for the motor. The bottom of the motor device is fixedly connected to the support base 6, and one side of the motor device is connected to the driving shaft 4. A battery box and a power line are arranged inside the support base 6, and the power line connects the motor and the battery box. A power switch 20 is arranged outside the support base 6. The motor device is driven, has a simple structure, a small size, is easy to use, and operates stably and persistently.

[0061] like Fig.12 As shown, the side wall of the cell culture well 2 is symmetrically provided with through holes 17, and each cell culture well 2 is equipped with a detachable circular sleeve 16, and the height of the sleeve 16 is slightly higher than the cell culture well 2, so that it is easy to be taken out from the cell culture well 2. The cell culture well 2 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 16 is first placed in the cell culture well 2, so that the through holes 17 on the side wall of the cell culture well 2 are blocked, and the culture medium in the cell culture well 2 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 2. After the cells are completely attached to the wall, the sleeve 16 is removed, so that the culture medium in the cell culture well 2 and the culture medium in the culture medium tank 13 can be merged into one.

[0062] like Fig.13 and Fig.14 As shown, the impeller 14 of the dynamic cell culture plate is a closed impeller, which is composed of front and rear cover plates and arc blades, and the arc blades are fixedly arranged between the front and rear cover plates. The closed impeller has high efficiency and can prevent the culture solution from splashing around, ensuring that the culture medium flows smoothly under the action of the impeller 14.

[0063] When the dynamic cell culture plate disclosed in the present invention is in use, the driving device 3 is started, and the driving device 3 drives the gear 8 to rotate through the driving shaft 4, and the gear 8 then drives the connecting rod 10 and the impeller 14 at the end of the connecting rod 10 to rotate. Under the action of the impeller 14, the culture medium will flow in the culture medium groove 13, so that the cells in the cell holes are in a dynamic environment, thereby realizing dynamic cell culture.

[0064] Implementation 2:

[0065] like Figure 1 and Figure 3 As shown, the difference between Embodiment 2 and Embodiment 1 is that the driving device 3 of the dynamic cell culture plate is different.

[0066] like Figure 3 and Figure 4 As shown, the driving device 3 of the dynamic cell culture plate of this embodiment is a clockwork device, the bottom of the clockwork device is fixedly connected to the support seat 6, one side of the clockwork device is connected to the driving shaft 4, and the other side of the clockwork device is provided with a clockwork handle 19. The clockwork device is driven without energy consumption, has a simple structure, a small size, and is easy to use.

[0067] Implementation 3:

[0068] like Figure 5 , Figure 6 and Fig.15 As shown, a dynamic cell culture plate includes a support plate 1, a cell culture hole 2, a driving device 3, a support seat 6, a driving shaft 4, a gear 8, a connecting rod 10, a culture medium tank 13, a reserved hole 15 and an impeller 14. The driving device 3 is fixedly connected to the support seat 6, the driving shaft 4 is connected to the connecting rod 10 through the gear 8, the driving device 3 drives the gear 8 and the connecting rod 10 to rotate through the driving shaft 4, the connecting rod 10 passes through the side wall of the culture medium tank 13 and the impeller 14 is arranged at the end of the connecting rod 10, and the cell culture hole 2 and the reserved hole 15 are arranged inside the culture medium tank 13.

[0069] The culture medium tank 13 of the dynamic cell culture plate is symmetrically arranged on the support plate 1 in a square shape, distributed on both sides of the driving shaft 4, the driving device 3 is arranged on the support seat 6, and the support seat 6 is arranged on the support plate 1, one end of the driving shaft 4 is connected to the driving device 3, and the other end of the driving shaft 4 is movably connected to the support rod 5 arranged on the support plate 1, and the surface of the driving shaft 4 is equidistantly provided with external threads 9, and the external threads 9 are meshed with the gear 8 fixedly arranged in the middle of the connecting rod 10, and the two ends of the connecting rod 10 respectively penetrate the corresponding side walls of the culture medium tank 13 and the end is provided with an impeller 14. The culture medium tank 13 is distributed in a square shape, which can adapt to more experimental occasions, and the number of the culture medium tank 13 can be increased or decreased in even numbers according to the needs of the experiment.

[0070] Two cell culture holes 2 and one reserved hole 15 are arranged in the culture medium tank 13 with a square distribution. The purpose of setting the two cell culture holes 2 is to culture two kinds of cells, and each cell culture hole 2 culture one kind of cell, so that the two kinds of cells live in the same flowing culture medium, and the interaction and influence between the two kinds of cells can be analyzed and observed. The reserved hole 15 can be arranged to form a special flow channel of the culture medium in the culture medium tank 13 by utilizing its outer shape structure, and cells can also be statically cultured in the reserved hole 15, or used as a control observation area for cell culture.

[0071] The connecting rod 10 of the dynamic cell culture plate is provided with a rubber sealing ring 18 at the position where it penetrates the side wall of the culture medium tank 13. In this way, during the rotation of the connecting rod 10, it can reduce the wear of the joint between the side wall of the culture medium tank 13 and the connecting rod 10, eliminate noise, and play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 10 to ensure the dynamic balance of the connecting rod 10.

[0072] The driving device 3 of the dynamic cell culture plate with the culture medium slots 13 arranged in a square shape is a motor device, and a battery is used to provide power for the motor. The bottom of the motor device is fixedly connected to the support seat 6, and one side of the motor device is connected to the driving shaft 4. A battery box and a power line are arranged inside the support seat 6, and the power line connects the motor and the battery box. A power switch 20 is arranged outside the support seat 6. The motor device is driven, and the structure is simple, the volume is small, the use is convenient, and the operation is long-lasting and stable.

[0073] like Fig.12 As shown, the cell culture wells 2 of the dynamic cell culture plate are symmetrically provided with through holes 17 on their side walls, and each cell culture well 2 is equipped with a detachable circular sleeve 16, the height of the sleeve 16 is slightly higher than the cell culture well 2, and is convenient for removal from the cell culture well 2. The cell culture well 2 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 16 is first placed in the cell culture well 2, so that the through holes 17 on the side walls of the cell culture well 2 are blocked, and the culture medium in the cell culture well 2 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 2. After the cells are completely adhered, the sleeve 16 is removed, so that the culture medium in the cell culture well 2 and the culture medium in the culture medium tank 13 can be merged into one.

[0074] like Fig.13 and Fig.14 As shown, the impeller 14 of the dynamic cell culture plate is a closed impeller, which is composed of front and rear cover plates and arc blades, and the arc blades are fixedly arranged between the front and rear cover plates. The closed impeller has high efficiency and can prevent the culture solution from splashing around, ensuring that the culture medium flows smoothly under the action of the impeller 14.

[0075] When the dynamic cell culture plate disclosed in the present invention is in use, the driving device 3 is started, and the driving device 3 drives the gear 8 to rotate through the driving shaft 4, and the gear 8 then drives the connecting rod 10 and the impeller 14 at the end of the connecting rod 10 to rotate. Under the action of the impeller 14, the culture medium will flow in the culture medium groove 13, so that the cells in the cell holes are in a dynamic environment, thereby realizing dynamic cell culture.

[0076] Implementation 4:

[0077] like Figure 5 and Figure 7 As shown, the difference between Embodiment 4 and Embodiment 3 is that the driving device 3 of the dynamic cell culture plate is different.

[0078] like Figure 7 and Figure 8As shown, the driving device 3 of the dynamic cell culture plate is a clockwork device, the bottom of the clockwork device is fixedly connected to the support seat 6, one side of the clockwork device is connected to the driving shaft 4, and the other side of the clockwork device is provided with a clockwork handle 19. The clockwork device is driven without energy consumption, has a simple structure, a small size, and is easy to use.

[0079] Implementation 5:

[0080] like Fig. 9 , Fig.11 and Fig.16 As shown, a dynamic cell culture plate includes a support plate 1, a cell culture hole 2, a driving device, a support seat 6, a driving shaft 4, a gear 8, a connecting rod 10, a culture medium tank 13, a reserved hole 15 and an impeller 14. The culture medium tank 13 of the dynamic cell culture plate is circularly arranged on the support plate 1, the driving device is installed inside the support seat 6, and the support seat 6 is arranged above the support plate 1 through a supporting column 7. One end of the driving shaft 4 passes through the bottom of the support seat 6 to connect to the driving device, and the other end of the driving shaft 4 is provided with a first bevel gear 11, and one end of the connecting rod 10 is provided with a second bevel gear 12 and meshes with the first bevel gear 11 provided at the end of the driving shaft 4. The driving device drives the first bevel gear 11 and the connecting rod 10 to rotate through the driving shaft 4, and the other end of the connecting rod 10 passes through the corresponding side wall of the culture medium tank 13 and an impeller 14 is provided at the end.

[0081] The culture medium tank 13 has a cell culture hole 2 and a reserved hole 15. The culture medium tank 13 is circularly distributed and can adapt to more experimental occasions. The number of culture medium tanks 13 can be increased or decreased according to the needs of the experiment, and only the number of connecting rods 10 with second bevel gears 12 needs to be increased or decreased at the same time.

[0082] The connecting rod 10 of the dynamic cell culture plate is provided with a rubber sealing ring 18 at the position where it penetrates the side wall of the culture medium tank 13. In this way, during the rotation of the connecting rod 10, it can reduce the wear of the joint between the side wall of the culture medium tank 13 and the connecting rod 10, eliminate noise, and play a sealing role to prevent the culture medium from leaking to the outside. At the same time, it can also serve as a fulcrum of the connecting rod 10 to ensure the dynamic balance of the connecting rod 10.

[0083] The driving device of the dynamic cell culture plate is a motor device, which uses a battery to provide power for the motor. The motor device is installed inside the support seat 6. The driving shaft 4 passes through the bottom of the support seat 6 and is connected to the motor device. A power switch 20 is arranged outside the support seat 6. A battery box and a power line are also arranged inside the support seat 6. The power line connects the motor and the battery box. The motor device is driven, has a simple structure, a small size, is easy to use, and operates stably and persistently.

[0084] like Fig.12As shown, the cell culture wells 2 of the dynamic cell culture plate are symmetrically provided with through holes 17 on their side walls, and each cell culture well 2 is equipped with a detachable circular sleeve 16, the height of the sleeve 16 is slightly higher than the cell culture well 2, and is convenient for removal from the cell culture well 2. The cell culture well 2 is suitable for culturing adherent cells. In the early stage of the experiment, the sleeve 16 is first placed in the cell culture well 2, so that the through holes 17 on the side walls of the cell culture well 2 are blocked, and the culture medium in the cell culture well 2 is in a closed and static state, and the cells can adhere to the bottom plate inside the cell culture well 2. After the cells are completely adhered, the sleeve 16 is removed, so that the culture medium in the cell culture well 2 and the culture medium in the culture medium tank 13 can be merged into one.

[0085] like Fig.13 and Fig.14 As shown, the impeller 14 of the dynamic cell culture plate is a closed impeller, which is composed of front and rear cover plates and arc blades, and the arc blades are fixedly arranged between the front and rear cover plates. The closed impeller has high efficiency and can prevent the culture solution from splashing around, ensuring that the culture medium flows smoothly under the action of the impeller 14.

[0086] When the dynamic cell culture plate disclosed in the present invention is in use, the driving device is started, and the driving device drives the first bevel gear 11 to rotate through the driving shaft 4, and the first bevel gear 11 drives the second bevel gear 12 to rotate, and the second bevel gear 12 drives the connecting rod 10 and the impeller 14 at the end of the connecting rod 10 to rotate. Under the action of the impeller 14, the culture medium will flow in the culture medium groove 13, so that the cells in the cell holes are in a dynamic environment, thereby realizing dynamic cell culture.

[0087] Implementation 6:

[0088] like Fig. 9 and Fig.10 As shown, the difference between Embodiment 6 and Embodiment 5 is that the driving device of the dynamic cell culture plate is different.

[0089] The driving device of the dynamic cell culture plate is a clockwork device, which is installed inside the support seat 6, the driving shaft 4 passes through the bottom of the support seat 6 and is connected to one side of the clockwork device, and the clockwork handle 19 passes through the top of the support seat 6 and is connected to the other side of the clockwork device. The clockwork device is driven, no energy consumption is required, the structure is simple, the volume is small, and it is easy to use.

[0090] In the figure, the description of the positional relationship is only for illustrative purposes and should not be understood as a limitation on this patent; obviously, the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A dynamic cell culture plate, comprising a support plate and a cell culture well, characterized in that: The dynamic cell culture plate also includes a driving device, a support seat, a driving shaft, a gear, a connecting rod, a culture medium tank, a reserved hole and an impeller, wherein the driving device is fixedly connected to the support seat, the driving shaft is connected to the connecting rod through a gear, the driving device drives the gear and the connecting rod to rotate through the driving shaft, the connecting rod penetrates the side wall of the culture medium tank and an impeller is arranged at the end of the connecting rod, and the cell culture hole and the reserved hole are arranged inside the culture medium tank; The side walls of the cell culture wells are symmetrically provided with through holes, and each cell culture well is equipped with a detachable circular sleeve, the height of the sleeve is higher than the cell culture well, so as to facilitate taking out the cell from the cell culture well; The culture medium tanks of the dynamic cell culture plate are symmetrically arranged on the support plate in a square shape and distributed on both sides of the drive. The driving device is arranged on the support seat, and the support seat is arranged on the support plate. One end of the driving shaft is connected to the driving device, and the other end of the driving shaft is movably connected to the support rod arranged on the support plate. External threads are arranged at equal distances on the surface of the driving shaft, and the external threads are meshed with the gears fixedly arranged in the middle of the connecting rod. The two ends of the connecting rod respectively penetrate the corresponding side walls of the culture medium tank and the ends are provided with impellers; The impeller is a closed impeller; the impeller is composed of front and rear cover plates and arc-shaped blades, and the arc-shaped blades are fixedly arranged between the front and rear cover plates; A rubber sealing ring is provided at the position where the connecting rod passes through the side wall of the culture medium tank; The driving device is a motor device, one side of which is connected to a driving shaft, or the driving device is a spring device, one side of which is connected to a driving shaft, and a spring handle is provided on the other side of the spring device; The culture medium tank can be set up as follows: In the first setting mode, a cell culture hole and a reserved hole are provided in the culture medium tank. The cell culture hole is used to culture adherent cells. The reserved hole can be used to form a special flow channel for the culture medium in the culture medium tank by utilizing its shape structure, or to statically culture cells in the reserved hole, or to serve as a control observation area for cell culture. In the second setting mode, two cell culture holes and one reserved hole are set in the culture medium tank. The purpose of setting the two cell culture holes is to culture two types of cells. Each cell culture hole cultures one type of cell. In this way, the two cells live in the same flowing culture medium, and the interaction and influence between the two cells can be analyzed and observed. The reserved hole can form a special flow channel for the culture medium in the culture medium tank by utilizing its external shape structure, and cells can also be statically cultured in the reserved hole, or it can be used as a control observation area for cell culture.

2. A dynamic cell culture plate, comprising a support plate and a cell culture well, characterized in that: The dynamic cell culture plate also includes a driving device, a support seat, a driving shaft, a gear, a connecting rod, a culture medium tank, a reserved hole and an impeller, wherein the driving device is fixedly connected to the support seat, the driving shaft is connected to the connecting rod through a gear, the driving device drives the gear and the connecting rod to rotate through the driving shaft, the connecting rod penetrates the side wall of the culture medium tank and an impeller is arranged at the end of the connecting rod, and the cell culture hole and the reserved hole are arranged inside the culture medium tank; The side walls of the cell culture wells are symmetrically provided with through holes, and each cell culture well is equipped with a detachable circular sleeve, the height of the sleeve is higher than the cell culture well, so as to facilitate taking out the cell from the cell culture well; The culture medium tank is circular and arranged on the support plate, the driving device is arranged inside the support seat, and the support seat is arranged above the support plate through a supporting column, one end of the driving shaft passes through the bottom of the support seat to connect to the driving device, and the other end of the driving shaft is provided with a first bevel gear, one end of the connecting rod is provided with a second bevel gear and meshes with the first bevel gear provided at the end of the driving shaft, and the other end of the connecting rod passes through the corresponding side wall of the culture medium tank and an impeller is provided at the end; The impeller is a closed impeller; the impeller is composed of front and rear cover plates and arc-shaped blades, and the arc-shaped blades are fixedly arranged between the front and rear cover plates; A rubber sealing ring is provided at the position where the connecting rod passes through the side wall of the culture medium tank; The driving device is a motor device, one side of which is connected to a driving shaft, or the driving device is a clockwork device, one side of which is connected to a driving shaft, and a clockwork handle is provided on the other side of the clockwork device; The culture medium tank is provided with a cell culture hole and a reserved hole.

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