In-vivo and in-vitro multi-component analysis method of fangji huangqi decoction

A technology of Fangji Huangqi Decoction and active ingredients is applied in the field of in vitro and in vivo multi-component analysis of Fangji Huangqi Decoction, which can solve the problems of complex formula components, difficult to reflect the overall level of the compound, and insufficient research on Fangji Huangqi Decoction, etc. The effect of good durability, high sensitivity and high analysis efficiency

CN112924572APending Publication Date: 2021-06-08SHANGHAI UNIV OF T C M
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-06-08

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Abstract

The invention relates to an in-vivo and in-vitro multi-component analysis method of fangji huangqi decoction. Specifically, a UPLC-MS / MS analysis method which is high in sensitivity, reliable in analysis quality and high in analysis efficiency and used for simultaneously determining 18 components of the fangji huangqi decoction, including magnoflorine, fangchinoline, tetrandrine, Calycosin-7-O-beta-D-glucoside, liquiritin, isoliquiritin, liquiritigenin, 5-O-methylvisammioside, ononin, calycosin, isoliquiritigenin, formononetin, atractylenolide III, atractylenolide II, astragaloside III, astragaloside A, astragaloside I and glycyrrhetinic acid is established, and a UPLC-MS / MS analysis method which is good in specificity, high in precision, small in matrix effect, high in recovery rate and high in stability and used for simultaneously determining seven main blood-entering components including magnoflorine, fangchinoline, tetrandrine, Calycosin-7-O-beta-D-glucoside, liquiritin, astragaloside A and glycyrrhetinic acid in blood plasma of the fangji huangqi decoction is established. The in-vivo and in-vitro multi-component analysis method lays a working foundation for quality control of the fangji huangqi decoction and in-vivo pharmacokinetics research.
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Description

technical field

[0001] The invention relates to the detection and analysis of multiple components in a sample, in particular to a method for analyzing multiple components in vivo and in vitro of Fangji Huangqi Decoction. Background technique

[0002] Fangji Huangqi Decoction is an ancient classic prescription in my country. It comes from the chapters "Synopsis of the Golden Chamber·Spasm and Dampness Syndrome and Treatment" and "Synopsis of the Golden Chamber·Water Qi Disease and Pulse Syndrome" by Han Zhang Zhongjing. , Atractylodes composition. In the prescription, Fangji is bitter and pungent, dispels wind, removes dampness, diuresis and reduces swelling, Astragalus nourishes qi, invigorates the spleen and lungs, and especially strengthens the surface and moves water. Atractylodes macrocephala invigorates the spleen and dries dampness, not only helps Astragalus invigorate qi and strengthen the exterior, but also helps Fangji to dispel dampness and diuresis. It is a prescr...

Examples

Embodiment 1

[0027] Quantitative analysis of 18 active ingredients in Fangji Huangqi Decoction in Example 1

[0028] 1 Instruments, materials and reagents

[0029] 1.1 Instrument

[0030] Dionex UPLC 3000 ultra-high performance liquid phase-TSQ quantum Access MAX triple quadrupole mass spectrometry system (Thermo, USA);

[0031] Waters Acquity UPLC BEH C18 column (2.1*100mm 1.7μm, Waters, USA);

[0032] FA2004N electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.);

[0033] CP225D electronic balance (Sartorius AG, Shanghai Precision Scientific Instrument Co., Ltd.);

[0034] Milli-Q type ultrapure water meter (Millipore company, the United States);

[0035] YC-18X Joyoung Induction Cooker (Shandong Joyoung Small Appliance Co., Ltd.);

[0036] SK250LHC ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.);

[0037] Micropipette 0.1-10 μL, 10-100 μL, 20-200 μL, 200-1000 μL (Eppendorf, Germany);

[0038] Centrifuge 5427R refrigerated centrifuge (Eppendor...

Embodiment 2

[0120] The establishment of the UPLC-MS / MS analytical method of multi-component simultaneous determination in embodiment 2 rat plasma

[0121] 1 Instruments, reagents, materials and animals

[0122] 1.1 Instrument

[0123] Dionex UPLC 3000 ultra-high performance liquid phase-TSQ quantum Access MAX triple quadrupole mass spectrometry system (Thermo, USA);

[0124] Waters Acquity UPLC BEH C18 column (2.1*100mm, 1.7μm, Waters, USA);

[0125] FA2004N electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.);

[0126] Milli-Q ultrapure water device (Millipore company, the United States);

[0127] LV concentrator (Biotage, USA);

[0128] QT-1 vortex mixer (Shanghai Qite Analytical Instrument Co., Ltd.);

[0129] Centrifuge 5427R refrigerated centrifuge (Eppendorf, Germany);

[0130] CP225D electronic balance (Sartorius AG, Shanghai Precision Scientific Instrument Co., Ltd.);

[0131] SK250LHC ultrasonic cleaner (Shanghai Kedao Ultrasonic Instrument Co., Ltd.);...