Liquid chromatography-mass spectrometry for determining atrazine, imidacloprid and metabolites thereof in leaves

A technology of liquid chromatography mass spectrometry and imidacloprid, which is applied in the field of liquid chromatography mass spectrometry for the determination of atrazine, imidacloprid and their metabolites in leaves, can solve the problems of complex process, large amount of organic solvent, and poor purification effect, and achieve The effect of simple method

CN112858542APending Publication Date: 2021-05-28李丽君
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2021-05-28

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Abstract

The invention belongs to the technical field of plant metabolite detection, and discloses a liquid chromatography-mass spectrometry method for determining atrazine, imidacloprid and metabolites thereof in leaves. The method comprises the steps of: using a QuEChERS method for analyzing various influence factors in the process of determining atrazine, imidacloprid and metabolite residues in plant leaves, and combining an HPLC-MS-MS method for detection; adding acetonitrile, carrying out vortex mixing, adding a salting-out material, carrying out vortex uniform mixing, and carrying out ultrasonic extraction and centrifugation at room temperature; and adding the supernatant into a centrifugal tube, adding a purifying agent anhydrous sodium sulfate and C18 for purification, uniformly mixing in a vortex manner, passing the supernatant through an organic phase microfiltration membrane, absorbing the filtrate, and determining by using a liquid chromatography mass spectrometer. The method has the advantages of simplicity and convenience in operation, good purification effect, high recovery rate, high sensitivity and the like, and can provide theoretical basis and technical support for monitoring residues of pesticides atrazine, imidacloprid and metabolites thereof in northeast black land agricultural crops and plant samples.
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Description

technical field

[0001] The invention belongs to the technical field of plant metabolite detection, and in particular relates to a liquid chromatography mass spectrometry method for determining atrazine, imidacloprid and their metabolites in leaves. Background technique

[0002] Atrazine (atrazine) chemical name: 2-chloro-4-diethylamino-6-isopropylamino-1,3,5-triazine is a triazine herbicide mainly used in corn It can control various broad-leaved weeds and annual grass weeds in crop fields such as , sorghum and sugarcane, and also has a certain inhibitory effect on perennial weeds. Due to its long residual period, high water solubility, large application amount and wide application range, atrazine has increasingly prominent impact on the environment. The U.S. Department of Health and Social Services reported that long-term exposure to atrazine can affect the human immune system, lymphatic system, reproductive system, and endocrine system, which may cause deformities and indu...

Examples

Embodiment Construction

[0068] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0069] Aiming at the problems existing in the prior art, the present invention provides a liquid chromatography-mass spectrometry method for determining atrazine, imidacloprid and their metabolites in plant leaves. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0070] Such as figure 1 As shown, the liquid chromatography-mass spectrometry method for determining atrazine, imidacloprid and their metabolites in plant leaves specifically includes:

[0071] S101, extraction and purification: Weigh plant leaves into a centrifuge tube, add acetonitrile, vortex mix, add salt...