A microplate

Abstract

The present invention discloses a microplate, including a protective shell, a light-transmitting hole is provided in the middle of the protective shell, a baffle is installed on the light-transmitting hole, an injection port is provided on the side of the light-transmitting hole, the protective shell and the baffle are both transparent structures, the inner side of the protective shell is connected to a mounting plate, an upper corridor is provided in the middle of the mounting plate, an observation hole is provided above the middle of the upper corridor, a corridor inlet is provided at one end of the upper corridor, and a corridor outlet is provided at the other end, the corridor outlet and the corridor inlet are both connected to the upper corridor through a channel, the corridor outlet is connected to the water outlet through a drainage channel, a detection chip is connected below the mounting plate, a lower corridor is provided on the detection chip, the lower corridor cooperates with the upper corridor to form a liquid flow channel, and a honeycomb hole is provided on the detection chip. The device is provided with a protective shell on the outside and is used by injection. During use, the test material can be prevented from being contaminated by the external environment, thereby improving the accuracy of the test.

Description

Technical Field

[0001] The invention relates to the technical field of microporous plates, and in particular to a novel microporous plate. Background Art

[0002] Microplates can be used in the fields of immunology and nucleic acid. Currently, there are many products that use microplates for cell detection and single-cell sequencing, and most of the microplates do not have corresponding covers. Therefore, cross contamination is very likely to occur during the detection and culture process, which will have a serious impact on the detection and culture results. Summary of the invention

[0003] The purpose of the present invention is to provide a new type of microporous plate to solve the problems mentioned in the background technology. To achieve the above purpose, the present invention provides the following technical solutions: a new type of microporous plate, including a protective shell, a light-transmitting hole is provided in the middle of the protective shell, a baffle is installed on the light-transmitting hole, an injection port is provided on the side of the light-transmitting hole, the protective shell and the baffle are both transparent structures, the inner side of the protective shell is connected to a mounting plate, an upper channel is provided in the middle of the mounting plate, an observation hole is provided above the middle of the upper channel, the observation hole is located below the baffle, and one end of the upper channel is provided with A channel inlet is provided at one end, and a channel outlet is provided at the other end. The channel outlet and the channel inlet are both connected to the upper channel through a channel. The channel outlet is connected to the water outlet through a drainage channel. The water outlet is opened at the end of the mounting plate. The channel inlet corresponds to the injection port and is connected through a channel. A detection chip is connected below the mounting plate. A lower channel is opened on the detection chip. The lower channel corresponds to the upper channel position and cooperates to form a liquid flow channel. The detection chip is provided with honeycomb holes.

[0004] Preferably, the lower corridor and the upper corridor are each provided with two, and the corresponding water outlets and injection ports are also provided with two groups.

[0005] Preferably, the drainage channel and the upper channel are located at different heights, and the opening of the water outlet is located at the bottom of the mounting plate.

[0006] Preferably, the mounting plate is a black structure, and a rubber sealing plug is installed on the injection port.

[0007] Preferably, the honeycomb holes are of regular hexagonal structure, and the honeycomb holes are micro-trapezoidal from top to bottom, with the inner wall of the hole and the bottom of the hole at an angle of 80 degrees. 0 ~90 0 The surface of the honeycomb holes is treated with hydrophilicity.

[0008] Preferably, the mounting plate and the detection chip are bonded.

[0009] Preferably, the honeycomb holes are opened on the bottom surface of the middle part of the lower channel by nano-imprinting technology and are located below the transparent plate.

[0010] Preferably, the honeycomb holes are opened on the entire surface of the detection chip by nano-imprinting or injection molding technology.

[0011] The technical effects and advantages of the present invention are as follows: the device is provided with a protective shell on the outside and is used by injection, which can prevent the test material from being contaminated by the external environment during use, thereby improving the accuracy of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 An exploded view of the present invention;

[0013] Figure 2 It is an assembly diagram of the present invention.

[0014] In the figure: 1-protective housing, 2-light transmission hole, 3-baffle, 4-injection port, 5-upper channel, 6-observation hole, 7-channel inlet, 8-channel outlet, 9-water outlet, 10-rubber sealing plug, 11-detection chip, 12-lower channel, 13-mounting plate, 14-drainage channel. DETAILED DESCRIPTION

[0015] In order to make the technical means for realizing the present invention, the creative features, the objectives and effects to be achieved easy to understand, the present invention is further explained below in conjunction with specific diagrams. In the description of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, an integral connection or a mechanical connection, or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal connection of two components.

[0016] Example 1

[0017] like Figure 1 and Figure 2A novel microporous plate shown in the figure comprises a protective shell 1, a light-transmitting hole 2 is provided in the middle of the protective shell 1, a baffle 3 is installed on the light-transmitting hole 2, an injection port 4 is opened on the side of the light-transmitting hole 2, the protective shell 1 and the baffle 3 are both transparent structures, the inner side of the protective shell 1 is connected to a mounting plate 13, an upper corridor 5 is opened in the middle of the mounting plate 13, an observation hole 6 is opened in the upper middle of the upper corridor 5, and the observation hole 6 is located below the baffle 3, a corridor inlet 7 is provided at one end of the upper corridor 5, and a corridor outlet 8 is provided at the other end, the corridor outlet 8 and the corridor inlet 7 are both connected to the upper corridor 5 through a channel, the corridor outlet 8 is connected to a water outlet 9 through a drainage channel 14, and the water outlet 9 is opened at the end of the mounting plate 13, the drainage channel 1 4 is located at different heights from the upper corridor 5, the opening of the water outlet 9 is located at the bottom of the mounting plate 13, the corridor inlet 7 corresponds to the injection port 4, and is connected through a channel, a rubber sealing plug 10 is installed on the injection port 4, a detection chip 11 is bonded and connected below the mounting plate 13, the mounting plate 13 is black, and it is convenient to absorb heat during bonding, a lower corridor 12 is opened on the detection chip 11, the lower corridor 12 and the upper corridor 5 are each provided with two, and the corresponding water outlet 9 and injection port 4 are also provided with two groups, the lower corridor 12 corresponds to the upper corridor 5, and cooperates to form a liquid flow channel, the detection chip 11 is opened with a honeycomb hole, the honeycomb hole is a regular hexagonal structure, the honeycomb hole is micro-trapezoidal from top to bottom, and the inner wall of the hole is 80° with the bottom of the hole. 0 ~90 0 The honeycomb hole surface is hydrophilic treated, and the honeycomb hole is opened on the bottom surface of the middle part of the lower corridor 12 by nanoimprinting technology, and is located below the transparent plate 7. When processing the detection chip 11, the structure of the lower corridor 12 is first processed on the detection chip 11 at a position corresponding to the position of the upper corridor 5, and then the honeycomb hole is printed on the bottom surface of the lower corridor 12 by nanoimprinting technology.

[0018] Example 2

[0019] like Figure 1 and Figure 2A novel microporous plate shown in the figure comprises a protective shell 1, a light-transmitting hole 2 is provided in the middle of the protective shell 1, a baffle 3 is installed on the light-transmitting hole 2, an injection port 4 is opened on the side of the light-transmitting hole 2, the protective shell 1 and the baffle 3 are both transparent structures, the inner side of the protective shell 1 is connected to a mounting plate 13, an upper corridor 5 is opened in the middle of the mounting plate 13, an observation hole 6 is opened in the upper middle of the upper corridor 5, and the observation hole 6 is located below the baffle 3, a corridor inlet 7 is provided at one end of the upper corridor 5, and a corridor outlet 8 is provided at the other end, the corridor outlet 8 and the corridor inlet 7 are both connected to the upper corridor 5 through a channel, the corridor outlet 8 is connected to a water outlet 9 through a drainage channel 14, and the water outlet 9 is opened at the end of the mounting plate 13, the drainage channel 1 4 is located at different heights from the upper corridor 5, the opening of the water outlet 9 is located at the bottom of the mounting plate 13, the corridor inlet 7 corresponds to the injection port 4, and is connected through a channel, a rubber sealing plug 10 is installed on the injection port 4, a detection chip 11 is bonded and connected below the mounting plate 13, the mounting plate 13 is black, and it is convenient to absorb heat during bonding, a lower corridor 12 is opened on the detection chip 11, the lower corridor 12 and the upper corridor 5 are each provided with two, and the corresponding water outlet 9 and injection port 4 are also provided with two groups, the lower corridor 12 corresponds to the upper corridor 5, and cooperates to form a liquid flow channel, the detection chip 11 is opened with a honeycomb hole, the honeycomb hole is a regular hexagonal structure, the honeycomb hole is micro-trapezoidal from top to bottom, and the inner wall of the hole is 80° with the bottom of the hole. 0 ~90 0 The surface of the honeycomb hole is hydrophilic, and the honeycomb hole is opened on the entire surface of the detection chip 11 by nanoimprinting or injection molding technology. When processing the detection chip 11, the honeycomb hole is first made on the entire surface of the detection chip 11 by nanoimprinting or injection molding technology, and then the lower channel 12 is processed on the detection chip 11 at a position corresponding to the position of the upper channel 5.

[0020] The process flow and working principle of the present invention are as follows: the detection chip 11 is bonded to the mounting plate 13, the lower channel 12 corresponds to the upper channel 5 in position, and cooperates to form a liquid flow channel, the protective housing 1 is installed above the mounting plate 13, the channel inlet 8 is connected to the injection port 4, and the transparent baffle 3 covers the observation hole 6 to form protection and facilitate observation. When conducting an experiment, such as a single-cell sequencing experiment, the rubber sealing plug 10 is opened, the solution is injected from the injection port 4, and the solution enters the liquid flow channel through the channel inlet 8. Each cell in the solution will enter a honeycomb hole, thereby achieving the purpose of separating single cells, and then the rubber sealing plug 10 is plugged to ensure that the solution on the detection chip 11 will not be contaminated during the experiment. After the experiment, the solution can be discharged through the channel outlet 7, the drainage channel 14 and the water outlet 9.

[0021] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microplate, comprising a protective housing, characterized in that: A light-transmitting hole is provided in the middle of the protective shell, a baffle is installed on the light-transmitting hole, an injection port is opened on the side of the light-transmitting hole, the protective shell and the baffle are both transparent structures, the inner side of the protective shell is connected to the mounting plate, an upper corridor is opened in the middle of the mounting plate, an observation hole is opened above the middle of the upper corridor, the observation hole is located below the baffle, a corridor inlet is provided at one end of the upper corridor, and a corridor outlet is provided at the other end, the corridor outlet and the corridor inlet are both connected to the upper corridor through a channel, the corridor outlet is connected to the water outlet through a drainage channel, the water outlet is opened at the end of the mounting plate, the corridor inlet corresponds to the injection port position, and is connected through the channel, a detection chip is connected below the mounting plate, a lower corridor is opened on the detection chip, the lower corridor corresponds to the position of the upper corridor, and cooperates to form a liquid flow channel, and a honeycomb hole is opened on the detection chip.

2. A microplate according to claim 1, characterized in that: The lower corridor and the upper corridor are each provided with two, and the corresponding water outlets and injection ports are also provided with two groups.

3. A microplate according to claim 1, characterized in that: The drainage channel and the upper channel are located at different heights, and the opening of the water outlet is located at the bottom of the mounting plate.

4. A microplate according to claim 1, characterized in that: The mounting plate is a black structure, and a rubber sealing plug is installed on the injection port.

5. A microplate according to claim 1, characterized in that: The honeycomb holes are of regular hexagonal structure, and are micro-trapezoidal from top to bottom. The inner wall of the hole and the bottom of the hole form an angle of 80° to 90°, and the surface of the honeycomb holes is treated with hydrophilicity.

6. A microplate according to claim 1, characterized in that: The mounting plate is bonded to the detection chip.

7. A microplate according to claim 1, characterized in that: The honeycomb holes are opened on the bottom surface of the middle part of the lower channel by nano-imprinting technology.

8. A microplate according to claim 1, characterized in that: The honeycomb holes are opened on the entire surface of the detection chip by nano-imprinting or injection molding technology.

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