X-ray excited nano photosensitizer and preparation method thereof

Abstract

The invention discloses an X-ray excited nano photosensitizer and a preparation method thereof. The composite material is obtained by compounding a pyridine cuprous iodide complex, methylene blue, polystyrene and sodium dodecyl benzene sulfonate according to a certain proportion and in a molecular stacking state through a microemulsion method. The method has the advantages of being simple, controllable in component and controllable in nanoparticle morphology. In the composite material, pyridine cuprous iodide has the performance of emitting light under excitation of X-rays, methylene blue has the photosensitizer performance of receiving fluorescence and generating singlet oxygen, polystyrene can enhance X-ray absorption and ensure that pyridine cuprous iodide is not affected by amino groups in a biological environment, and therefore the photodynamic effect is further enhanced; in addition, the sodium dodecyl benzene sulfonate on the surfaces of the particles enhances the controllability and stability of the morphology of the particles; due to the fact that the penetration depth of the X-ray in the tissue is not limited, the material is expected to solve the problem that the penetration depth of the tissue is small in photodynamic therapy, and the material can be used for photodynamic therapy of deep tumors.

Description

technical field

[0001] The invention relates to the technical field of medical functional materials, in particular to an X-ray excited nano photosensitizer and a preparation method thereof. Background technique

[0002] Photodynamic therapy (PDT) refers to the use of light of a specific wavelength to irradiate a photosensitizer in the presence of oxygen to initiate a photosensitization reaction and generate singlet oxygen. The strong oxidizing singlet oxygen acts on the biomacromolecules in tumor cells to produce cytotoxicity, resulting in severe damage to cells and triggering apoptosis of tumor cells. Traditional photodynamic therapy (PDT) uses visible light or ultraviolet light to excite photosensitizers (PSs), but the penetration depth of visible light or ultraviolet light in the tissue is relatively shallow, and it cannot reach deep into the tissue to treat deep tumors.

[0003] There is no limit to the penetration depth of X-rays in tissues, and X-ray scanning is usual...

Examples

Embodiment 1

[0040] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0041] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0042] (2) Take 1mL Cu 4 I 4 py 4 Mix the solution with PS in chloroform, add 1 mg photosensitizer methylene blue (MB) to it;

[0043] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (1 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then ultrasonically treated for 20 minutes;

[0044] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is colorles...

Embodiment 2

[0047] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0048] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0049] (2) Take 1mL Cu 4 I 4 py 4 Mix the solution with PS in chloroform, add 1 mg photosensitizer methylene blue (MB) to it;

[0050] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (2 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then ultrasonically treated for 20 minutes;

[0051] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is colorles...

Embodiment 3

[0054] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0055] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0056] (2) Take 1mL Cu 4 I 4 py 4 The solution was mixed with PS in chloroform, and 1 mg of photosensitizer methylene blue (MB) was added thereto.

[0057] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (5 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then sonicated for 20 minutes;

[0058] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is ...

technical field

[0001] The invention relates to the technical field of medical functional materials, in particular to an X-ray excited nano photosensitizer and a preparation method thereof. Background technique

[0002] Photodynamic therapy (PDT) refers to the use of light of a specific wavelength to irradiate a photosensitizer in the presence of oxygen to initiate a photosensitization reaction and generate singlet oxygen. The strong oxidizing singlet oxygen acts on the biomacromolecules in tumor cells to produce cytotoxicity, resulting in severe damage to cells and triggering apoptosis of tumor cells. Traditional photodynamic therapy (PDT) uses visible light or ultraviolet light to excite photosensitizers (PSs), but the penetration depth of visible light or ultraviolet light in the tissue is relatively shallow, and it cannot reach deep into the tissue to treat deep tumors.

[0003] There is no limit to the penetration depth of X-rays in tissues, and X-ray scanning is usual...

Examples

Embodiment 1

[0040] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0041] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0042] (2) Take 1mL Cu 4 I 4 py 4 Mix the solution with PS in chloroform, add 1 mg photosensitizer methylene blue (MB) to it;

[0043] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (1 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then ultrasonically treated for 20 minutes;

[0044] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is colorles...

Embodiment 2

[0047] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0048] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0049] (2) Take 1mL Cu 4 I 4 py 4 Mix the solution with PS in chloroform, add 1 mg photosensitizer methylene blue (MB) to it;

[0050] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (2 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then ultrasonically treated for 20 minutes;

[0051] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is colorles...

Embodiment 3

[0054] A preparation method of X-ray excited nano photosensitizer, the steps are as follows:

[0055] (1) 30mg Cu 4 I 4 py 4 Add 150mg polystyrene (PS) to a 5mL glass bottle, add 3mL chloroform, stir at room temperature for 1 hour, and sonicate for 30 minutes to make Cu 4 I 4 py 4 Dissolve PS in chloroform;

[0056] (2) Take 1mL Cu 4 I 4 py 4 The solution was mixed with PS in chloroform, and 1 mg of photosensitizer methylene blue (MB) was added thereto.

[0057] (3) Dissolve 1 mL of Cu 4 I 4 py 4 1. The chloroform solution of PS and MB was quickly injected into 10 mL of an aqueous solution of sodium dodecylbenzenesulfonate (5 mg / mL) at room temperature, stirred vigorously at 1000 rpm for 1 hour, and then sonicated for 20 minutes;

[0058] (4) Slowly stir the ultrasonically treated emulsion at room temperature for 48 hours to completely volatilize the chloroform, centrifuge at 12,000 rpm for 10 minutes, and wash with deionized water 4 times until the supernatant is ...

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