Wheat transgenic event IND-ØØ412-7

Abstract

The present invention relates to wheat plants, seeds or parts thereof comprising event IND‑ØØ412‑7, commercial products obtained from said seeds, recombinant DNA molecules contained in event IND‑ØØ412‑7, DNA primers and probes that can be used to detect event IND‑ØØ412‑7, DNA detection kits, methods for producing wheat plants tolerant to abiotic stress and methods for detecting the presence of DNA belonging to wheat event IND‑ØØ412‑7 in a sample.

Description

Field of the Invention

[0001] The present invention relates to the fields of plant production, plant breeding, and agriculture. More specifically, it relates to the wheat transgenic event IND-ØØ412-7, nucleotide sequences related to the wheat transgenic event IND-ØØ412-7, plants, plant parts, seeds, cells, agricultural products, and detection and production methods. Background of the Invention

[0003] Improving crop yields and their characteristics has become essential to meet food needs. Thanks to the development of biotechnology integrated with agriculture, new crops have been developed that are able to adapt to different environmental and / or ecological conditions.

[0004] During plant growth, plants are exposed to a variety of abiotic stresses: drought, salinity, low and high temperatures, excessive radiation, low nutrient availability, soil compaction that hinders root development, and more (Duque et al., 2013; Sayed, 2003). Each of these can, at some point, affect plant growth and development, as well as yield. It's reasonable to assume that any of these environmental factors could occur during the life cycle of a field crop. When this happens, complex response mechanisms are triggered, which will be reflected in the measurements performed, as they result from the integration of these stress effects.

[0005] One of the most common technologies for mitigating negative environmental impacts on crops is based on transgenic events, which involve the insertion of a gene of interest into the genome of a target crop. However, the generation and selection of commercially suitable transgenic events requires extensive research, analysis, and characterization of a large number of individual transformation events. This results in the selection of events that contain the desired trait, enabling the development of desired phenotypic and agronomic characteristics suitable for commercial purposes without negatively impacting other crop characteristics.

[0006] This process requires the generation of transgenic events that will be characterized molecularly and phenotypically in order to identify and select events expressing the heterologous gene of interest in view of the desired phenotype to be obtained.

[0007] Event selection involves a phase of laboratory development and trials conducted under controlled conditions in the field and / or in greenhouses. It is necessary to analyze the response to events at multiple locations and under various environmental conditions over multiple years to select events that meet the desired phenotypic and genetic characteristics, as well as the desired commercial traits.

[0008] The present invention features commercially suitable events of this type, which enable new advantageous traits in wheat.

[0009] There are a variety of genes that can be used to generate commercially suitable events. A gene whose expression is particularly interesting is the gene encoding the transcription factor HAHB4.

[0010] HAHB4 (which stands for Sunflower ( Helianthus annuus HaHB4 (Helicobacter pylori homeobox-4) is a sunflower transcription factor belonging to the HD-Zip family. HaHB4 gene expression is regulated at the transcriptional level by external environmental factors, such as water availability and soil salinity, as well as the plant hormones abscisic acid and ethylene, which are associated with these factors.

[0011] Patent AR81216B2 discloses a gene, HaHB4, that is induced by water deficiency and abscisic acid and encodes a sunflower transcription factor type HD-Zip. The patent also discloses the isolation and characterization of the gene and its introduction into the model plant Arabidopsis thaliana ( Arabidopsis thaliana However, it does not report on commercially interesting transgenic plants carrying the events of the present invention, nor on their advantageous properties with respect to associated abiotic stresses occurring under agricultural conditions.

[0012] There is no mention of expressed genes in patent AR81216B2 HaHB4 The events were specifically selected to retain the main trait of interest, i.e. drought tolerance, without affecting other agronomic characteristics.

[0013] Furthermore, application AR090110A1 discloses a modified gene that is inducible by water deficiency and abscisic acid and encodes a modified sunflower transcription factor type HD-Zip (in particular mod1HaHB4) HaHB4 (in particular HaHB4.2 ). This publication discloses the modified HaHB4.2 The production and characterization of expression constructs and their introduction into the model plant Arabidopsis thaliana. HaHB4.2 Generation and selection of transgenic events in soybean, wheat, and maize. Detailed Description of the Invention

[0015] The present invention provides abiotic stress resistant wheat plants comprising event IND-ØØ412-7. These plants provide advantages in growing under adverse environmental conditions, thereby allowing for higher yields.

[0016] More specifically, the present invention relates to a wheat event designated IND-ØØ412-7, having representative seeds registered with the American Type Culture Collection (ATCC) under Accession No. PTA-126141, and its progeny.

[0017] The present invention also includes a wheat plant comprising event IND-ØØ412-7 represented by the sequences SEQ ID No.: 1 and SEQ ID No.: 2.

[0018] The transgenic insertions present in the events of the present invention and in the registered seeds contain selectable bar Marker genes and genes conferring abiotic stress tolerance HaHB4 . Derived from Streptomyces hygroscopicus ( Streptomyces hygroscopicus )of bar Gene encoding PAT (phosphinothricin acetyltransferase) protein. HaHB4 Derived from the sunflower plant Helianthus annuus species (sp. Helianthus annuus ), and encodes a protein type HD-Zip I having a protein domain of the homeodomain type, which is associated with a leucine zipper that primarily provides abiotic stress tolerance to drought. Regulation of the gene of interest can be directed by different promoter sequences with different expression levels, sensitivities, and tissue specificities. Experts in the field know that any promoter or nucleic acid terminator that directs or regulates the expression of the gene of interest can be used without affecting the essence of the present invention. Specifically, the events developed in the present invention include those for providing glufosinate-ammonium herbicide resistance. bar The maize ubiquitin 1 gene promoter (prUBI-1) and Tnos In addition, the event again contains the maize ubiquitin 1 gene promoter (prUBI-1) and Tnos terminator to regulate HaHB4 Expression of coding regions ( Figure 1 and Figure 2 ).

[0019] Other aspects of the present invention include progeny of wheat plants comprising the wheat event IND-ØØ412-7, seeds and / or regenerable parts of the plants, seeds and progeny, and food products derived therefrom for human or animal consumption. The present invention also includes parts of plants comprising event IND-ØØ412-7, including, but not limited to, pollen, ovules, flowers, shoots, roots, leaves, vegetative nuclei of cells and other plant cells comprising event IND-ØØ412-7. The present invention also relates to wheat plants comprising the wheat event IND-ØØ412-7, characterized by tolerance to a variety of abiotic stresses, such as drought, salinity, low and high temperatures, excess radiation, low nutrient availability, soil compaction, and the like, and different combinations of these factors.

[0020] The present invention relates in part to the cultivation of abiotic stress tolerant plants. In addition, it includes novel events for the transformation of wheat plants comprising a polynucleotide as described herein inserted into a specific site within the wheat genome, said polynucleotide providing specific genetic and phenotypic characteristics.

[0021] In certain embodiments, the event / polynucleotide can be "stacked" with other traits, including, for example, agronomic traits and herbicide and / or insect tolerance. However, the present invention includes plants containing a single event as described herein.

[0022] Additional traits can be stacked within the plant genome or in the same locus as event IND-ØØ412-7, for example, by crossing plants, retransformation of transgenic plants comprising event IND-ØØ412-7, or adding new traits by integration directed by homologous recombination.

[0023] In one embodiment, the invention comprises two wheat chromosomal loci located on chromosome 2. In certain embodiments, the targeting loci comprise heterologous nucleic acid. Figure 3 between the flanking sequences defined in (SEQ ID Nos: 7, 8, 9 and 10).

[0024] In one embodiment, the present invention comprises a method for producing a transgenic wheat plant comprising inserting a heterologous nucleic acid at one or several specific locations within chromosome 2.

[0025] Specifically, the method comprises transforming cells or cell cultures with the DNA sequences described in SEQ ID No.: 1 and SEQ ID No.: 2 and regenerating the cells in a stable manner to start new whole plants.

[0026] The transformation of the plant cells can be carried out by a variety of techniques, whether physical, viral or chemical, including biolistics, electroporation, bacterial transformation or a combination of these techniques. All of these techniques are well known to experts in the field.

[0027] Specifically, the present invention uses a gene gun to propel DNA plasmid molecules with sequences of interest ( Figure 2 ), more precisely, using plasmids pIND4-HB4 and pIND4-BAR ( Figure 1 and Figure 2 ; SEQ ID No: 3 and SEQ ID No: 4) bombardment.

[0028] In addition, the present invention provides a test for detecting the presence of this event in a wheat sample. The test can be based on the DNA sequence of the recombinant construct inserted into the wheat genome, as well as the genomic sequence based on the flanking insertion site. Also provided are test kits and conditions for conducting the test.

[0029] Thus, the present invention relates, in part, to the cloning and analysis of DNA sequences belonging to all inserts, or portions thereof, and flanking regions (in transgenic wheat lines). These sequences are unique. Event-specific primers can be generated based on these insert and flanking (and combined) sequences. PCR technology has demonstrated that by analyzing the amplicons produced by these sets of event-specific primers, these events can be identified. Therefore, these and other related procedures can be used to unambiguously identify wheat lines containing the events of the present invention.

[0030] The present invention also relates to PCR assays, including quantitative real-time (qPCR) or end-point PCR, to detect IND-ØØ412-7 events, amplicons, and fragments thereof.

[0031] The present invention also provides a DNA molecule comprising a portion of a contiguous nucleotide sequence of SEQ ID No.: 5 and SEQ ID No.: 6, wherein the portion of the contiguous nucleotide sequence is sufficient to enable it to function as a DNA probe, which hybridizes to a DNA molecule comprising a nucleotide sequence selected from SEQ ID No.: 5 and SEQ ID No.: 6 under stringent conditions and does not hybridize to a DNA molecule that does not comprise a nucleotide sequence selected from SEQ ID No.: 5 and SEQ ID No.: 6 under stringent conditions.

[0032] In some cases, the probe used can be labeled with a molecule that emits a detectable signal. Examples of such molecules are fluorescent dyes. That is, oligonucleotides that exhibit fluorescent dyes at both ends and have a sequence complementary to a portion of the DNA fragment to be amplified. Examples include FAM, TET, HEX, JOE, CAL Fluor®, Quasar®, and Pulsar® dyes.

[0033] The present invention also discloses a DNA molecule pair consisting of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein the first and second DNA molecules each contain a portion of consecutive nucleotides of SEQ ID No.: 5 and SEQ ID No.: 6, which portion of consecutive nucleotides is sufficient to function as a DNA probe, and if the DNA probe is used in an amplification reaction together with DNA derived from event IND-ØØ412-7, it will produce a diagnostic DNA amplicon for the wheat transgenic event IND-ØØ412-7 in the sample.

[0034] The present invention also describes a method for detecting the presence of DNA obtained from event IND-ØØ412-7 in a sample. The method comprises matching the sample with DNA molecules used as probes and primers, subjecting them to identical stringent hybridization conditions, and then detecting hybridization of the DNA probe to DNA in the sample amplified using specific primers. This hybridization indicates the presence of DNA derived from the transgenic wheat event IND-ØØ412-7 in the sample.

[0035] The present invention also provides a method for detecting the presence of a DNA molecule derived from the wheat transgenic event IND-ØØ412-7 in a sample, which comprises matching a DNA preparation derived from the sample with an oligonucleotide pair used as primers to produce an amplification reaction, wherein the reaction is sufficient to produce a DNA amplicon comprising a sequence selected from SEQ ID No.: 5 and SEQ ID No.: 6, to detect the presence of the DNA amplicon in the reaction, wherein the presence of the DNA amplicon indicates the presence of a DNA molecule derived from IND-ØØ412-7 in the sample.

[0036] In addition, the present invention provides a DNA detection kit comprising at least one DNA molecule having a number of consecutive nucleotides of SEQ ID No.: 5 and SEQ ID No.: 6, said number being sufficient to enable it to function as a primer or specific DNA probe to detect the presence of DNA derived from the wheat transgenic event IND-ØØ412-7, wherein the DNA detection confirms the presence of the event in the sample.

[0037] The invention also features a wheat plant, seed, cell, or plant part comprising a nucleic acid molecule selected from the group consisting of SEQ ID No.: 5 and SEQ ID No.: 6. The invention further provides a wheat plant, seed, cell, or plant part that is tolerant to abiotic stress. Furthermore, the invention features a wheat plant, seed, cell, or plant part whose genome, when tested using a DNA amplification method, produces an amplicon comprising a DNA molecule selected from the group consisting of SEQ ID No.: 5 and SEQ ID No.: 6.

[0038] The present invention further provides a wheat plant or seed, wherein the wheat plant or seed is produced from the wheat transgenic event IND-ØØ412-7, or is a hybrid or heterozygote having at least one parent derived from the wheat transgenic event IND-ØØ412-7.

[0039] The present invention further provides non-living plant material comprising a recombinant DNA molecule selected from the group consisting of SEQ ID No.: 5 and SEQ ID No.: 6.

[0040] Additionally, the present invention provides a commodity product produced from the wheat transgenic event IND-ØØ412-7, comprising a nucleic acid molecule selected from the group consisting of SEQ ID No.: 5 and SEQ ID No.: 6, wherein detection of the nucleotide sequence in a sample derived from the commodity product indicates that the commodity product is derived from the wheat transgenic event IND-ØØ412-7. The present invention further provides a commodity product selected from the group consisting of whole or processed seeds, meal, flour, flakes, bioethanol, biogas, or other biomaterials. The present invention also features a method for producing a commodity product by obtaining a wheat plant or part thereof comprising the wheat transgenic event IND-ØØ412-7, and producing the wheat commodity product from the wheat plant or part thereof.

[0041] The present invention provides a method for producing a wheat plant that is tolerant to abiotic stress by crossing a plant of a wheat transgenic event IND-ØØ412-7 comprising a nucleic acid molecule selected from the group consisting of SEQ ID No.: 5 and SEQ ID No.: 6 with a second wheat plant to produce seed, collecting the seeds produced from the cross, growing the seeds to produce a plurality of progeny plants, and selecting progeny plants that are tolerant to abiotic stress. <110> Baisai Biotech Co., Ltd. <120> Wheat transgenic event IND-ØØ412-7 <130> 22108 <160> 14 <170> PatentIn version 3.5 <210> 1 <211> 47126 <212> DNA <213> Artificial sequence <220> <223> Long inserts without flanking regions <400> 1 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 60 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 120 catactcttc ctttttcaat attattgaag catttatcag ggttatattgtc tcatgagcgg 180 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 240 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaatag 300 gcgtatcacg aggccctttc gtctcgcgg tttcggtgat gacggtgaaa acctctgaca 360 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 420 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 480 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 540 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 600 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 660 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 720 caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag 780 cattgcatgt ctaagttata aaaattacc acatattttt tttgtcacac ttgtttgaag 840 tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat 900 agtactacaa father gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaact ctatttagt ttttttattt aatatttag atataataata gaataaata aagtgactaa aatattaaca aatacccttt 1320. 1320. 1320. 1320. 1320. 1320. 1320. 1320. 1320. 1320 taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc 1440 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacgggggga 1560 ttcctttccc accgctcctt cgctttccct tcctcgccccg ccgtaataa tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc 1680 tcccccaaat ccacccgtcg gcgtacgccg ctcgtcctcc cccccccccc ctctctacct 1740 tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt 1800 tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc 1860 tgtacgtcag acacgtaacg tccggagagt tcgtaaaccg atcatccgat tcttcaccgc 1920 tgccactact actagttttc tcttgatgct tttcggctac atttctcagc acctccaatt 1980 gattgagtag ggcctgattc tctttcttta gagactcgga tttagacgca agcgtctcgt 2040 agttatgctt tagcgcgtta tactcttgct caatctgcct cgactttgat cgcgcgcgtt 2100 tgttctggaa ccatatcgcc acttgacgag gatgaagccc gagtttatgt gccaactggt 2160 gtttcatcct taactcgggt ctcgactgtg tctcaaacat gtactctagg aaacttattt 2220 gtttgtcggt aaatcgtctc cgcccctcgt ttcggttctt cctggtggtt gttacttgtt 2280 gaagagacat ggatccgggt gtgatcctct atagtcgacc tgcagaagta acaccaaaca 2340 acagggtgag catcgacaaa agaaacagta ccaagcaaat aatagcgta tgaaggcagg 2400 gctaaaaaaa tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa 2460 ataattataa aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga 2520 atagatgctg catatgccat catgtatatg catcagtaaa acccacatca acatgtatac 2580 ctatcctaga tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca 2640 cacatacagt tccaaaatta ataaatacac caggtagtttt gaaacagtat tctactccga 2700 tctagaacga atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa 2760 gcatctctgt atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat 2820 atttccatcc atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc 2880 caaaattaat aaatccacca ggtagtttga aacagaattg gaattcgtaa tcatggtcat 2940 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 3000 gcataaagtg taagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 3060 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3120 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 3180 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3240 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3300 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3360 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3420 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3480 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3540 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3600 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3660 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3720 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 3780 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3840 cttgatccgg caaacaaacc accgctggta gcggtggtttt ttttgtttgc aagcagcaga 3900 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3960 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4020 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4080 aaacttggtc tgacagtttt caaatatgta tccgctcatg agacaataac cctgataaat 4140 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa cattccgtg tcgcccttat 4200 tcccttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 4260 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 4320 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 4380 agttctgcta tgtggcgcgg tattatccg tattgacgcc gggcaagagc aactcggtcg 4440 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 4500 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataccatga gtgataacac 4560 tgcggccaac ttactctga caacgatcgg aggaccgaag gagctaccg ctttttgca 4620 siacatgggg gatcatgtaa ctcgccttga tcgttggga ccggagctga atgaagccat 4680 accaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaaacgt tgcgcaact 4740 attackactggc gaactactta ctctagcttc ccggcaacaa ttatagact ggatggaggc 4800 ggataaagtt gcaggaccac ttctgcctc ggccctccg gctggctggt ttattgctga 4860 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 4920 taagccctcc cgtacgtag ttactac gacgggagt caggcaacta tggatgaacg 4980 aaatagacag atcgctgaga taggtgccctc actgattaag cattggtaaa actgtcagac 5040 caagtttact catatatact ttagattgat ttaaaacttc atttttatt taaaaggatc 5100 taggtgaaga tcctttttga taatctcatg accaaatcc cttaacgtga gtttcgttc 5160 cactgagcgt cagaccccgt agaagagatcg ttcaacatt tggcaataaa gtttcttaag 5220 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 5280 gcatgtaata attaacatgt aatgcatgac gttattattg agatgggttt ttatgattag 5340 5400 taaattatcg cgcgcggtgt catctatgtt actagatcgg atccatgagc ccagaacgac 5460 gcccggccga catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca 5520 accactacat cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggaggt 5580 ggacggacga cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg 5640 gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggc acgcaacgcc tacgactgga 5700 cggccgagtc gaccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc 5760 tctacaccca cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca 5820 tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg 5880 gcatgctgcg ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc 5940 tggacttcag cctgccggta ccgccccgtc cggtcctgcc cgtcaccgag atcgatcgtt 6000 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta 6060 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt 6120 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag 6180 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac 6240 tagatcggaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6300 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6360 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6420 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6480 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6540 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6600 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6660 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6720 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6780 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6840 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6900 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6960 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7020 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7080 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7140 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7200 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7260 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7320 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7380 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7440 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7500 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7560 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7620 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7680 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7740 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7800 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7860 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7920 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7980 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8040 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8100 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8160 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8220 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8280 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8340 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8400 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8460 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8520 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8580 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 8640 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8700 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 8760 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 8820 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 8880 caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc cccctctag agataatgag 8940 cattgcatgt ctaagttata aaaaattacc acatatttt ttgtcacac ttgtttgaag 9000 tgcagtttat ctatctttat acatatttt aaactttact ctacgaataa tataatctat 9060 agtactacaa tatatcagt gttttagaga atcatacaa tgacagtta gataggtct 9120 aaaggacaat tgagtattt vakaacagga ctctacagtt ttatctttt agtgtgcatg 9180 tgttctcctt ttttttgca atagcttca cctatataat acttcatcca ttttagt 9240 acatccattt agggtttagg gttaatggtt ttatagact aattttttta gtacatcttat 9300 ttttattctt ttagcctct aaattaagaa actaaact ctattttagt ttttattt 9360 aaaatttag attaaaata gaaaaata agtgactaa aattaaca atacccttt 9420 aaaaattta aaaaactaag gaacatttt tcttgtttcg agtagataat gccagcctgt 9480 taaacgccgt cgacgagtct aacggacacc aaccagcgaa cgcagcgt cgcgtcgggc 9540 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc 9600 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga 9660 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga 9720 ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc 9780 ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc 9840 tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc 9900 ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta 9960 cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt 10020 acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt 10080 tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt 10140 tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact 10200 tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt 10260 tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg gatttattaa 10320 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg 10380 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga 10440 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt 10500 ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt 10560 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag 10620 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca 10680 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata 10740 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt 10800 ttagccctgc cttcatacgc tatttattg cttggtactg tttctttgtgt cgatgctcac 10860 cctgttgttt ggtgttactt ctgcagggat ccatgagccc agaacgacgc ccggccgaca 10920 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg 10980 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc 11040 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg 11100 gcatcgccta cgcgggcccc tggaaggcac gcaacgccta cgactggacg gccgagtcga 11160 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc 11220 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca 11280 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg 11340 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc 11400 tgccggtacc gccccgtccg gtcctgcccg tcaccgagat cgatcgttca aacatttggc 11460 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc 11520 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat 11580 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat 11640 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatctgctac 11700 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 11760 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 11820 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 11880 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 11940 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 12000 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 12060 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 12120 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 12180 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 12240 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 12300 atacatattt gaatgtattt agaaaaataa acaataggg gttccgcgca catttccccg 12360 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 12420 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 12480 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 12540 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 12600 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 12660 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 12720 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 12780 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 12840 caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag 12900 cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag 12960 tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat 13020 13080. 13080. 13080. 13080. 13080. 13080. 13080. 13080. 13080 aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaact ctatttagt ttttttattt aatatttag atataataata aatatta aagtgactaa aatacca aatacccttt 13440. aagaattaa aaaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt ttgttgaata gaatacaaac cctagaaaaa caaggcacat ctcggcgccg ccaatagaag gcagaaaccc tagccgtcat ttttcttgaa gaatagaag cgacgtcgat aggaagatac ttaaatgttg aatccaagca cctagata tgagttagat tagaagaaaa ctatcaactt cgaaatcaag aagaaatgtt ttattctgtt gcataaaact ttcgcgtgga 13740. gaagctctgt tcagagcccg ggtagccact gggcacgtct cctagatact ggactcatcg gacgctctcc tcgagccatt aatatggtcc gtcctgtaga aaccccaacc cgtgaaatca 13800 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc 13860 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tctcctgttc cgaccctgcc 13920 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 13980 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctcggca tccggtcagt 14040 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg 14100 tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga 14160 ccacgcattg atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc 14220 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc 14280 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact 14340 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga 14400 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc 14460 ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa 14520 actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga 14580 taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca 14640 aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga 14700 gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca 14760 ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca 14820 ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt 14880 tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga 14940 ccgcaaaccg aagtcggcgg ctcgctcagt gtgtgtgact ctgctggtgc tggggaatgg 15000 agctcggggc accgtatttg tagcgcgcgc gccgctgtac gtggcgccct ggcgcggcta 15060 cgtggagggg agctgcgtgc cagaggggag gcgggccgcc acgtcgcacg gccgtgctta 15120 tcgggccatc ggcgaggtgc gtgccagctt gggcgtcgcg tgtcgtggag cgctcctgct 15180 cgcggcggag ctggttgagt ggattgcgat gcggtgcttg gtacagagct cgatggacaa 15240 ctcgaggaga gcgtccgatg agtccagtat ctaggagacg tgcccagtgg ctacccgggc 15300 tctgaacaga gcttctccac gcgaaagttt tatgcaacag aataaaacat ttcttcttga 15360 tttcgaagtt gatagttttc ttctaatcta actcattatt ctaggtgctt ggattcaaca 15420 tttaagtatc ttcctatcga cgtcgctcta tattcttcaa gaaaaatgac ggctagggtt 15480 tctgccttct attggtcatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 15540 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 15600 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 15660 tctaaagtat atatgagtaa acttggtctg acagttttac caatgcttaa tcagtgaggc 15720 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 15780 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 15840 cccacgctca ccggctccag attackcag c ataaaccag ccacccggaa gggccgagcg 15900 cagaagtggt cctgcaactt tatccgcctc catccagtct atttattgtt gccgggaagc 15960 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 16020 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 16080 gcgagttaca tgatccccca tgttgtgcaa aaagcggtt agctccttcg gtcctccgat 16140 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 16200 ttctcttact gtcatgccat ccgtagatg cttttctgtg actggtgagt actcaaccaa 16260 gtcattctga gatagtgta tgcggcgacc gagttgctt tgcccgggt caatacggga 16320 taataccggcg ccacatagca gaactttaaa agtgctcatc attggaaac gttctcggg 16380 gcgaaaactc tcaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 16440 acccaactga tcttcagcat cttttactt caccagcgtt tctggtgag caaaaacagg 16500 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 16560 cttcctttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 16620 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 16680 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 16740 cacgaggccc ttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 16800 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 16860 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 16920 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 16980 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 17040 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 17100 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 17160 tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa tgagcattgc 17220 atgtctaagt tataaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga father ctatagtact acaatgtacg ccgctcgtcc tcccccccc cccctctcta ccttctctag atcggcgttc 17400 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt 17460 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt 17520 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc 17580 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt 17640 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt 17700. ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc 17760 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg 17940 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta 18000 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga 18060 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact 18120 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta 18180 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg 18240 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc 18300 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagtcgac 18360 tcgtagagga tccatcccga ttaggaaagt agagcatgag cccagaacga cgcccggccg 18420 acatccgccg tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca 18480 tcgagacaag cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg 18540 acctcgtccg tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg 18600 ccggcatcgc ctacgcgggc ccctggaagg cacgcaacgc ctacgactgg acggccgagt 18660 cgaccgtgta cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc 18720 acctgctgaa gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc 18780 ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc 18840 gggcggccgg cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca 18900 gcctgccggt accgccccgt ccggtcctgc ccgtcaccga gatcatatga atagatgctg 18960 catatgccat catgtatatg catcagtaaa acccacatca acatgtatac ctatcctaga 19020 tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca cacatacagt 19080 tccaaaattt ataaatacac caggtagttt gaaacagtat tctactccga tctagaacga 19140 atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa gcatctctgt 19200 atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat atttccatcc 19260 atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc caaaattaat 19320 aaatccacca ggtagtttga aacagaattc tactccgatc tagaacgacc gcccaaccag 19380 accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc 19440 acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa 19500 aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt ccccaaagag 19560 aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa 19620 cgctagcagc acggatctaa cacaaacacg gatctaacac aaacatgaac agaagtagaa 19680 ctaccgggcc ctaaccatgg accggaacgc cgatctagag aaggtagaga gggggggg 19740 gggaggacga gcggcgtacg ccgacgggtg gatttggggg agatctggtt gtgtgtgtgt 19800 gcgctccgaa caacacgagg ttggggaaag agggtgtgga gggggtgtct atttattacg 19860 gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga atcccccgta gctgccggtg 19920 ccgtgagagg aggaggc cgcctgccgt gccggctcac gtctgccgct ccgccacgca 19980 atttctggat gccgacagcg gagcaagtcc aacggtggag cggaacctc gagaggggtc 20040 cagaggcagc gagagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg 20100 gttcgctggt tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac 20160 tcgaaacaag aaaaatgttt ccttagtttt tttaatttct taaagggtat tgtttaatt 20220 tttagtcact ttattttatt ctattttata tctaaattat taaaaaaa actaaaaa 20280 gagttttagtt tttctttatt tagaggcta atagaata atagatgta ctaaaaaaat 20340 tagtcttaa aaccattaa ccctaaaccc taaatgtg tactataa atggtg 20400 tattatatag gtgaagctat ttgcaaaaaaaggagaac acatgcacac taaaagata 20460 aaactgtaga gtcctgttgt caaatactc aattgtcctt tagaccatgt ctaactgttc 20520 atttatatga ttctctaaaa cactgatatt attgtagtac tatagatt attattcgta 20580 gagtaagtt t'aatatatg t'a'a'ata gataactgc acttcaaca agtgtgacaa 20640 aaaaaatatg tggtaatttt ttataactta gacatgcaat gctcattatc tctagagagg 20700 ggcacgaccg ggtcacgctg cactgcaggc atgcaagctt gcactggccg tcgttttaca 20760 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 20820 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 20880 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 20940 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 21000 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 21060 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 21120 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 21180 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 21240 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 21300 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 21360 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 21420 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 21480 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 21540 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 21600 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 21660 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 21720 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 21780 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 21840 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 21900 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 21960 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 22020 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 22080 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagagtc aggcaactat 22140 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 22200 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 22260 aaggaatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 22320 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 22380 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 22440 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 22500 gataccaaat actgtcctt tagtgtagcc gtagttaggc caccacttca agaactctgt 22560 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 22620 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 22680 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 22740 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 22800 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 22860 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 22920 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 22980 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 23040 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 23100 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 23160 tctccccgcg cgttggccga ttcatttatg cagctggcac gacaggtttc ccgactggaa 23220 agcgggcagt gagcgcaacg caatttatgt gagttagctc actcattagg caccccaggc 23280 tttacacttt atgcttccgg ctcgtatgtt aagcttgcat gcctgcagtg cagcgtgacc 23340 cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca 23400 catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta 23460 aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa 23520 tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac 23580 tctacagttt tatcttttta gtgtgcatgt gttctcctttt ttttttgcaa atagcttcac 23640 ctataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt 23700 ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa 23760 actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa 23820 agtgactaaa attaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt 23880 cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca 23940 accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc 24000 gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc 24060 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctctcct 24120 cctctcacgg caccggcagc tacggggat tcctttccca ccgctcctt gctttccctt 24180 cctcgcccgc cgtaataaat agacacccga actgtatgtg tgtgtcatac atcttcatag 24240 ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt 24300 tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag 24360 tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg 24420 atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt 24480 atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca 24540 gggatccatg tctcttcaac aagtaacaac caccaggaag aaccgaaacg aggggcggag 24600 acgatttacc gacaaacaaa taagtttcct agagtacatg tttgagacac agtcgagacc 24660 cgagttaagg atgaaacacc agttggcaca taaactcggg cttcatcctc gtcaagtggc 24720 gatatggttc cagaacaaac gcgcgcgatc aaagtcgagg cagattgagc aagagtataa 24780 cgcgctaaag cataactacg agacgcttgc gtctaatcc gagtctctaa agaaagagaa tcaggcccta ctcaatcaat tggaggtgct gagaaatgta gccgaaaagc atcaagagaa aactagtagt agtggcagcg gtgaagaatc ggatgatcgg tttacgaact ctccggacgt tatgtttggt caagaatga atgttccgtt ttgcgacggt tttgcgtacc ttgaagaagg aaacagtttg ttggagattg aagaacaact gccagacctt caaaagtggt gggagttcga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata father tgattacgt father father tgtaatgcat gacgttattt atgagatggg tttttatgat taggtcccg caattataca tttaatacgc gatagaaac aaaatatagc gcgcaaacta ggataatta tcgcgcgcgg tgtcatctat gttactagat cggaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 25500 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 25560 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 25620 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 25680 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 25740 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaaatc gacgctcaag 25800 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 25860 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 25920 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 25980 cgttcgctcc aagctggggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 26040 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 26100 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 26160 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 26220 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 26280 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 26340 agatccttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 26400 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 26460 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt gatcgttggg 26520 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 26580 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 26640 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 26700 cggctggctg gttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 26760 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 26820 gtcaggcaac tatggatgaa cgaatagac agatcgctga gatagaactg tcagaccaag 26880 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 26940 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 27000 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 27060 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggttttgt ttgccggatc 27120 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 27180 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 27240 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 27300 ttaccgggt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 27360 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 27420 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 27480 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 27540 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 27600 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc gacgctgggc cgccttttta 27660 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 27720 tctgtggata accgtattac cgcctttgag tgagctgata gtgtcacgca gatcctatga 27780 aatttggtcg atgattgtct tttatggatc cgttcagacc aagtcttcgt gtgtctatgt 27840 tatcgtgcct ttaaattgaa tcctttcgat ttatgcttct ttttgtcagc ggtggttgat 27900 cttctgatgt gctggttaca aagggtctta gcataacaat tttccaattg tctactacaa 27960 caagttttgt ctaacttcga tgaagcctag gtgatgacga tttcaacttg cttcggtgct 28020 tgtaactgtt attagatggt ctacggatct aaatgtaact tctattattt gttgtgtttt 28080 ttgtattatt atgatggatg atgaatagat taaaagatcc cgcaaataaa accttaaatg 28140 ataaatgtca cacgtcacgt gcatggcact ccggccctaa tgccttttgt agctattcta 28200 gtaacgcaag gatagcaaat ttccgtgaca cgacgcaagt ttttgttaaa aataattaa 28260 agcaagaaaa ttgtcatact tgctaacgaa aattgccatc cttgcctatt catcatccat 28320 cataataata caaaaaacac aacaataat agaagttaca tttagatccg tagaccatct 28380 aataacagtt acaagcaccg aagcaagttg gtgccgtctg cttcgcttgg cccgacgcga 28440 cgctgctggt tcgctggttg gtgtccgtta gactcgtcga cggcgtttaa caggctggca 28500 ttatctactc gaaaagaa aaatgtttcc ttagtttttt taatttctta aagggtattt 28560 gtttaatttt tagtcacttt attttattct attttattc taaattatta aataaaaaaa 28620 ctaaaataga gttttagttt tcttaattta gaggctaaaa tagaataaaa tagatgtact 28680 aaaaactcaa ttgtccttta gaccatgtct aactgttcat ttatatgatt ctctaaggca 28740 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 28800 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 28860 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 28920 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 28980 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 29040 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 29100 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 29160 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 29220 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattacca atgcttaatc 29280 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 29340 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 29400 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 29460 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 29520 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 29580 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt catcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 29700. cctccgaata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 29820. tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 29880. cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaatg ttgaatactc atactcttcc tttttcaata ttattgagc atttatcagg gttattgtct catgagcgga 30060. tacatatttg aagatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag 30180 cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt 30240. gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc 30300 ggccgtccag tcgcgcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac 30360 ctcgccgtat ggatgaagta ttatataggt gaagctttt gcaaaaaaa agggacaac 30420 atgcacacta aaagataa actgtagagt cctgttgtca aaatactcaa ttgtccttta 30480 gaccatgtct aactgttcat ttatatgat ctctaaaaca ctgatattat tgtagtacta 30540 taacttat tattcgtaga gtaagtttta tattatagta window taactgcac 30600 ttcaacaag tgtgacaaaa aaatatgtg gtaatttttt ataacttaga catgcaatgc 30660 tcattatctc tagagagggg cacgaccgggg tcacgctgca ctgcaggcat gcaagcttct 30720 gcagaaaaaaaaaaaaaaaaaggaaaaaaaaacaaaaaaaaacaaaa 30780 atagcgtat gaaggcaggg ctaaaaaaat ccacatatag ctgctgcata tgccatcatc 30840 caagtatatc aagatcaaa tattattaaa acatacttgt tattatat agataggtac 30900 tcaggttag agcatatgaa tagagctgc atatgccatc atgtatatgc atcagtaaaa 30960 cccacatcaa catgtatacc tatcctagat cgatatttcc atccatctta aactcgtaac 31020 tatgaagatg tatgacacac acatacagtt ccaaaattaa taaatacacc aggtagtttg 31080 aaacagtatt ctactccgat ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc 31140 acaaccaagc gaacaaaaag catctctgta tatgcatcag taaaacccgc atcaacatgt 31200 atacctatcc tagatcgata tttccatcca tcatcttcaa ttcgtaacta tgaatatgta 31260 tggcacacac atacagatcc aaaattaata aatccaccag gtagtttgaa acagaattct 31320 actccgatct agaacgaccg cccaaccaga ccacatcatc acaaccaaga caaaaaaaag 31380 catgaaaaga tgacccgaca aacaagtgca cggcatatat tgaaataaag gaaaagggca 31440 aaccaaaccc tatgcaacga aacaaaaaaa atcatgaaat cgatcccgtc tgcggaacgg 31500 ctagagccat cccaggattc cccaaagaga aacactggca agttagcaat cagaacgtgt 31560 ctgacgtaca ggtcgcatcc gtgtacgaac gctagcagca cggatctaac acaaacacgg 31620 atctaacaca aacatgaaca gaagtagaac taccgggccc taaccatgga ccggaacgcc 31680 gattagaga aggtagagag gggggggggg ggaggacgag cggcgtacct tgaagcggag 31740 gtgccgacgg gtggatttgg gggagatctg gttgtgtgtg tgtgcgctcc gaacacacg 31800 aggttggga agagggtgt ggaggggtg tctattt acggcggggg aggaagggaa 31860 agcgaaggag cggtgggaaa ggaatccccc gtagctgccg gtgccgtgag aggaggagga 31920 ggccgcctgc cgtgccggct cacgtctgcc gctccgccac gcaatttctg gatgccgaca 31980 gcggagcaag tccaacggtg gagcggaact ctcgagaggg gtccagaggc agcgacagag 32040 atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg ctggttcgct ggttggtgtc 32100 cgttagactc gtcgacggcg ttacaggc tggcattatc tactcgaac aagaaaaatg 32160 tttccttagt ttttaatt tcttaaggg tatttgttta atttttagtc actttattt 32220 attctatttt atatctaaat tattaata aaaaactaa atagagtttt agttttctta 32280 atttagaggc taaaatagaa taaatagat gtactaaaaaattagtcta taaaaaccat 32340 taaccctaaa ccctaaatgg atgtactaat aaaatggatg aagttatta taggtgaagc 32400 tatttgcaaa aaaaaaggag aacacatgca cactaaaaag ataaaactgt agagtcctgt 32460 tgtcaaaata ctcaattgtc cttagacca tgtctaactg ttcatttata tgattctcta 32520 aaacactgat attattgtag tactatagat tatattattc gtagagtaaa gtttaaatat 32580 atgtataaag atagataaac tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat 32640 tttttataac ttagacatgc aatgctcatt atctctagag aggggcacga ccgggtcacg 32700 ctgcactgca ggcatgcaag cttgcactgg ccgtcgtttt acaacgtcgt gactgggaaa 32760 acctggcgt tacccaactt aatcgccttg cagcacatcc cccttcgcc agctggcgta 32820 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 32880 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 32940 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 33000 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 33060 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 33120 gacgaaaggg cctcgtgata cgcctttt tataggttaa tgtcatgata ataatggttt 33180 cttagacgtc aggtggcact tttcgggggaa atgtgcgcgg aacccctatt tgttttttt 33240 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 33300 aatattgaaa aaagaagagt atgagtattc aacatttccg tgtcgccctt attcccttt 33360 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 33420 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 33480 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 33540 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 33600 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 33660 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 33720 acttacttct gacaacgatc ggaggaccga aggagctaac cgctttttg cacaacatgg 33780 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 33840 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 33900 gcgaactact tactctagct tcccggcaac attaataga ctggatggag gcggataaag 33960 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gttattgct gataaatctg 34020 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 34080 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaatagac 34140 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 34200 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 34260 tccttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 34320 cagaccccgt agaaaagatc aaaggatctt cttgagatcc ttttttctg cgcgtaatct 34380 gctgcttgca aaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 34440 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 34500 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 34560 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 34620 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 34680 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 34740 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 34800 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 34860 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 34920 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 34980 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 35040 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 35100 cagtgagcga ggaagcggaa gagcgcccaa tacgcaacc gcctctcccc gcgcgttggc 35160 cgattcatta atgcagctgg cacgacaggt ttccgactg gaagcgggc agtgagcgca 35220 acgcaattaa tgtgagttag ctcactcatt agcacccca ggctttacac tttagcttc 35280 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 35340 accatgatta cgaattccga tcgttcaaac atttggcaat aaagttttctt agattgaat 35400 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta 35460 ataattaaca tgtaatgcat gacgttattt atgagatgggg ttttgat tagagtcccg 35520 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaacta ggataatta 35580 tcgcgcgcgg tgtcatctat gttactagat cgatctcggt gacgggcagg accggacggg 35640 gcggtaccgg caggctgaag tccagctgcc agaacccac gtcatgccag ttcccgtgct 35700 tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca 35760 cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca 35820 gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg 35880 agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg 35940 cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc 36000 gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga 36060 ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg 36120 tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat ggatccaagc ttgcatgcct 36180 gcagtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag 36240 ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc 36300 tttatacata tatttaaact ttactctacg aataatataa tctatagtac tacaataata 36360 tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt 36420 atttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt 36480 ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc catttagggt 36540 ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat tctattttag 36600 cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata 36660 aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa 36720 ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg 36780 agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg 36840 gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc accgttggac 36900 ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg 36960 caggcggcct cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc 37020 tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt 37080 ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc 37140 cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc tctctacctt 37200 ctctagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt 37260 gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg gatgcgacct 37320 gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga 37380 tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag 37440 ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat 37500 cttttcatgc tttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta 37560 gatcggagta gaattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg 37620 tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag 37680 gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg 37740 cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga 37800 atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac 37860 atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt 37920 tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc 37980 taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg 38040 atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc cctgccttca 38100 tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt 38160 tacttctgca gggatccatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg 38220 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca 38280 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg 38340 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg 38400 gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc 38460 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg 38520 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc 38580 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc 38640 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc 38700 gtccggtcct gcccgtcacc gagatctgat ccgtcgacct gcagatcgtt caaacatttg 38760 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt 38820 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 38880 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 38940 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggaa 39000 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 39060 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 39120 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 39180 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 39240 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 39300 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 39360 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 39420 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 39480 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 39540 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 39600 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 39660 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 39720 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 39780 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 39840 cggctacact agaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 39900 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 39960 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 40020 ttctacgggg tctgacgctc agtgggaacga aaactcacgt taagggattt tggtcatgag 40080 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 40140 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 40200 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 40260 aactacgata cgggaggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 40320 acgctcaccg gctccagatt tatcagcaat aaccagcca gccggaaggg ccgagcgcag 40380 aagtggtcct gcaactttat ccgcttccat ccagtctatt aattgttgcc gggaagctag 40440 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 40500 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 40560 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 40620 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcatattc 40680 tcttactgtc atgccatccg tagatgctt ttctgtgact ggtgagtact caaccaagtc 40740 attctgagaa tagtgtatgc ggcgaccgag ttgcttgc ccggcgtcaa tacggtaa 40800 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 40860 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 40920 caacgatct tcagcatctt ttacttcac cagcgttct gggtgagca aaacaggaag 40980 gcaaaatgcc gcaaaaagg gataaggggc xacggaaa tgttgaatac tcatactctt 41040 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 41100 tgaatgtatt tagaaaaata aaaaatagg ggttccgcgc acatttcccc gaaagtgcc 41160 acctgacgtc windowaacca ttttatcat gatacacc tataaaaata ggcgtatcac 41220 gaggcccttt cgtctcgcgc gtttcggtga tgacggtga aacctctgac acatgcagct 41280 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 41340 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 41400 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 41460 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 41520 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 41580 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg caagcttgca 41640 tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag cattgcatgt 41700 ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag tgcagtttat 41760 ctatctttat acatatattt aaactttact ctacgaataa tataatctat agtactacaa 41820 taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat 41880 tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt 41940 tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt 42000 agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaact ctatttagt ttttttattt aaattttag 42180. 42180. 42180. 42180. 42180. 42180. 42180. 42180 aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc 42300 agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt 42360 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttccttttccc 42480 accgctcctt cgctttccct tcctcgcccg ccgtaataa tagacacccc ctccacaccc 42540 tctttcccca acctcgtgtt gttcggagcg cacacacaca cacagatc tcccccaaat 42660. cccccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca 42720 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc 42780 gacctgtacg tcagacacgt tctgattgct aacttgccag tgttctctt tggggaatcc 42840 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg 42900 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg 42960 gtcatcttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg 43020 ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct 43080 gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga 43140 tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt 43200 gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga 43260 gtagaatact gtttcaaact acctggtgta ttttattaatt ttggaactgt atgtgtgtgt 43320 catacatctt catagttacg agtttaagat ggatggaaat atcgatctag gataggtata 43380 catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata 43440 tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga 43500 tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc 43560 cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt 43620 ggtgttactt ctgcaggtcg actctagagg atccatcgat taggaagtaa ccatgagccc 43680 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 43740 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 43800 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 43860 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 43920 cgactggacg gccgagtcga ccgtgtacgt ctcccccgc caccagcgga cgggactggg 43980 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 44040 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 44100 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 44160 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 44220. ctgatccgtc gacctgcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta father tgtaatgcat gacgttattt atgagatggg tttttatgat taggtcccg 44460. snow snow snow snow snow snow snow snow snow snow tcgcgcgcgg tgtcatctat gttactagat cggaattcgt aatcatggtc atagctgttt 44520. cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 44760 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 44820 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 44880 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 44940 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 45000 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 45060 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 45120 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 45180 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 45240 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 45300 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 45360 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 45420 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 45480 agaaaaaaag gatctcaaga agatccttg atcttttcta cggggtctga cgctcagtgg 45540 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 45600 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 45660 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 45720 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 45780 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 45840 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 45900 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 45960 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 46020 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 46080 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 46140 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 46440 ttcaccagcg tttctgggtg agcaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 46620. 46620. 46620. 46620. 46620. 46620. 46620. 46620. 46620. 46620. 46620. 46620 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctata aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg tagcggatg ccggggagcag acaagcccgt caggcggcgt cagcgggtgt tggcgggtgt 46860 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 46920 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc 46980 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 47040 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 47100 cgacgttgta aaacgacggc cagtgc 47126 <210> 2 <211> 19598 <212> DNA <213> Artificial Sequence <220> <223> Short Insert without Flanking Regions <400> 2 ttggaacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 60 actacttact ctagcttccc ggtaacaatt aatagactgg atggaggcgg ataaagttgt 120[[ID=z8]] aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 180 cggactctag gcttcccggg caacaaatta attagactgg atggagggat aaagtttgca 240 gaccactttc tgcgctcggc ccttccgctg ggctgttcta tgcccgctga aatcaccagt 300 ctctctctac aaatctatct ctctctaa taatgtgtga gtagttccca 360 years ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 480 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 540. ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gcttattacgc 600 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaac gacggccagt gcaagcttgc atgcctgcag tgcagcgtga 720 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt 900. 900. 900. 900. 900. 900. 900. 900. 900. 900. 900 aatcatata atgaacagtt agacatggtc taaggacaa ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc acctatataa tacttcatcc attttag tacatccatt taggtttag ggttaatggt 1080 ttttatagac taattttt agtacatcta ttttattcta ttttagccctc taaattaaga 1140 aaactaaaac tctattttag ttttttatt taadaattta gatataaat agaataaat 1200 aaagtgacta aaatttaac aaataccctt tagaaatta aaaaacta ggaacattt 1260 ttcttgtttc gagtagataa tgccagcctg ttaacgccg tcgacgagtc taacggac 1320 caccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg 1380 1440 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctccctc 1500 ctcctctcac ggcaccggca gctacgggg attccttcc caccgctcct tcgctttccc 1560 ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt 1620 tgttcggagc gcacacac acaaccagat ctccccaaa tccaccgtc gggcgtacgcc 1680 gctcgtccctc ccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt 1740 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc 1800 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac 1860 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc 1920 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc 1980 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttcctgt 2040 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 2100 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2160 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 2220 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 2280 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 2340 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 2400 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 2460 tcgttgggaa ccggagctga atgaagccat accaacgac gagcgtgaca ccacgatgcc 2520 tgtagcaatg gcaacaacgt tgcgcaact atttactgtt attgccggga aaagtgtacg 2580 tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccctgca gaagtacac 2640 caacacag ggtgagcatc ghaaaagaa acagtaccaa gcaataat agcgtgaa 2700 ggcagggcta aaaaaatcca catatagctg ctgcatatgc catcatccaa gtatatcaag 2760 atcaaaataa tttaaaaca tacttgttta ttataga taggtactca aggttagagc 2820 attgaatag atgctgcata tgccatcatg tatatgcatc agtaaaaccc acatcacat 2880 gtatacctat cctagatcga tattccatc catcttaaac tcgtaactat gagatgtat 2940 gadocacaca tacagttcca aaattaataa atacaccagg tagtttgaa cagtattcta 3000 ctccgatcta gaacgaatga acgaccgcccc aaccacacca catcatcaca accaagcgaa 3060 caaaagcat ctctgtatat gcatcagtaa aacccgcatc aacatgtata cctatcctag 3120 aagtactata gattatatta ttcgtagagt aaagtttaaa gattata aagatagata 3180 aactgcactt caaacaagtg tgacaaaaaa aatatgtggt aattttttat aacttagaca 3240 tgcaatgctc attatctcta gagaggggca cgaccgggtc acgctgcact gcaggcatgc 3300 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 3360 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 3420 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 3480 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 3540 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 3600 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 3660 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 3720 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 3780 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattacca 3840 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 3900 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 3960 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4020 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4080 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4140 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4200 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4260 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 4320 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 4380 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 4440 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 4500 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 4560 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4620 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4680 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4740 tctcatgagc ggatacatat ttgaaaactg tcagaccaag tttactcata tatactttag 4800 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4860 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4920 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taagccaata gaaggcagaa 4980 accctagccg tcatttttct tgaagaatat agagcgacgt cgataggaag atacttaaat 5040 gttgaatcca agcacctaga ataatgagtt agattagaag aaaactatca acttcgaaat 5100 caagaagaaa tgttttattc tgttgcataa aactttcgcg tggagaagct ctgttcagag 5160 cccgggtagc cactgggcac gtctcctaga tactggactc atcggacgct ctcctcgagt 5220 tgtccatcga gctctgtacc aagcaccgca tcgcaatcca ctcaaccagc tccgccgcga 5280 gcaggagcgc tccacgacac gcgacgccca agctggcacg cacctcgccg atggcccgat 5340 aagcacggcc gtgcgacgtg gcggcccgcc tcccctctgg cacgcagctc ccctccacgt 5400 agccgcgcca gggcgccacg tacagcggcg cgcgcgctac aaatacggtg ccccgagctc 5460 cattccccag caccagcaga gtcacacaca ctgagcgagc taaaccatca ggaagtgatg 5520 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg 5580 aaaagtgtac gtatcaccgt ttgtgccgga atccatcgca gcgtaatgct ctacaccacg 5640 ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac 5700 gcgtctgttg actggcaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg 5760 gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg 5820 cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag 5880 acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa 5940 cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg 6000 gacttgcgtg gcaaaggatt cgataacgtg ctgttaccaa tgcttaatca gtgaggcacc 6060 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 6120 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 6180 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6240 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6300 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6360 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6420 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6480 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6540 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6600 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6660 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6720 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6780 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6840 gcaaaatgcc gcaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6900 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6960 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 7020 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 7080 gaggcccttt cgtctcgcgc gttcggtga tgacggtgaa aacctctgac acatgcagct 7140 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 7200 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 7260 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggaaaata 7320 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 7380 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 7440 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg caagcttgca 7500 tgcctgcagt gcagcgtgac ccggtcgtgc ccctctag agataatgag cattgcatgt 7560 ctaagttata aaaaattacc acatatttt ttgtcacac tgtttgaag tgcagtttat 7620 ctatctttat acatattattt aactttact ctacgataa tataatctat agtactacaa 7680 taatatcagt gttttagaga atcatataaa tgacagtta gatagtct aaaggacaat 7740 tgagtatttt vakaacagga ctctacagtt ttatctttt agtgtgcatg tgttctcctt 7800 ttttttgca atagcttca cctatataat acttcatcca ttttagt acatccattt 7860 agggtttagg gttaatggtt tttatagact aattttttta gtacatcttat tttattctat 7920 tttagcctct aaattaagaa actaaaact ctattttagt ttttatttt aataatttag 7980 attaaaata gataaata aagtgactaa aattaaca atacccttt aagaattta 8040 aaaaactaag gaacatttt tctgtttcg agtagataat gccagcctgt taaacgccgt 8100 cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc 8160 agacggcacg gcatctctgt ctgaatagaa tacaaccct agaaaacaa ggcacatctc 8220 ggcgccgcca atagaaggca gaaaccctag ccgtcatttt tcttgaagaa tatagagcga 8280 cgtcgatagg aagatactta aatgttgaat ccaagcacct agaataatga gttagattag 8340 aagaaaacta tcaacttcga aatcaagaag aaatgtttta ttctgttgca taaaactttc 8400 gcctggagaa gctctgttca gagcccgggt agccactggg cacgtctcct agatactgga 8460 ctcatcggac gctctcctcg agttgtccat cgagctctgt accaagcacc gcatcgcaat 8520 ccactcaacc agctccgccg cgagcaggag cgctccacga cacgcgacgc ccaagctggc 8580 acgcacctcg ccgatggccc gataagcacg gccgtgcgac gtggcggccc gcctcccctc 8640 tggcacgcag ctcccctcca cgtagccgcg ccagggcgcc acgtacagcg gcgcgcgcgc 8700 tacaatacg gtgccccgag ctccattccc cagcaccagc agagtcacac acactgagcg 8760 agctaaacca tggtatagcc gccctgatgc tccatcactt cctgattatt gacccacact 8820 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc 8880 ggtataaaga cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg 8940 gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt 9000 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg 9060 tcgagttttt tgatttcacg ggttggggtt tctacaggac gctattaatt gttgccggga 9120 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9180 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9240 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9300 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttcact gtcagaccaa 9360 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 9420 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 9480 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 9540 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 9600 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 9660 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 9720 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga tagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaggcgga caggtatccg gtaagcggca gggtcggac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatcttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 10080 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 10140 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 10200 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgcg cgcccaatac gcaaaccgcc tctccccgcg 10320. cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg cacccaggc tttacacttt 10440 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 10500 agctatgacc atgattacga attccgatct agtaacatag atgacaccgc gcgcgataat 10560 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg 10620 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca 10680 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc 10740 aatcttaaga aactttattg ccaaatgttt gaacgatctg caggtcgacg gatcagatct 10800 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac 10860 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat 10920 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca 10980 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca 11040 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg 11100 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg 11160 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt 11220 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc 11280 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc 11340 tcatggatcc agctccgccg cgagcaggag cgctccacga cacgcgacgc ccaagctggc 11400 acgcacctcg ccgatggccc gataaggcag agcgaggtat gtaggcggtg ctacagagtt 11460 cttgaagtgg tggcctaact acggctcac tagaaggaca gtatttggta tctgcgctct 11520 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 11580 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 11640 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 11700 ttaagggatt ttggtcatga gattatcaaa aggatcttc acctagatcc ttttaaatta 11760 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 11820 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 11880 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 11940 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 12000 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 12060 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 12120 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 12180 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 12240 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 12300 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 12360 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 12420 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 12480 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacagga ggcaaaatgc cgcaaaaaag ggaataggg cgacacgga atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attack tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggccctctc gctattacgc cagctggcga aaggggtg 13200 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 13260 gacggccagt gcaagcttgc atgcctgcag tgcagcgtga cccggtcgtg cccctctcta 13320 gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt tttgtcaca 13380 cttgtttgaa gtgcagttta tctatcttta tacatatatt taaacttac tctacgaata 13440 ataatcta tagtactaca atatatcag tgttttagag atcatataa atgacagtt 13500 agacatggtc taaaggacaa ttgagtattt tgacaacagg actctacagt ttatctttt 13560 tagtgtgcat gtgttctcct ttttttgc aaatagctc acctatataa tacttcatcc 13620 attattag tacatccatt taggttttag ggttaatggt ttttatagac taattttttt 13680 agtacatcta ttttattcta ttttagcctc taaattaaga aaactaaaac tctattttag 13740 ttttttatt taadaattta gatataaaat agaatgacta aaaatthaac 13800 aaataccctt taagaatta aaaaaactaa ggaacattt ttctgttttc gagtagataa 13860 tgccagcctg ttaaacgccg tcgacgagtc taacggacac caaccagcga accagcagcg 13920 tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc 13980 tcgagagttc cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc 14040 ggagcggcag acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca 14100 gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa 14160 atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac 14220 acaaccagat ctcccccaaa tccacccgtc ggcgtacgcc gctcgtcctc cccccccccc 14280 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt 14340 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca 14400 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg 14460 ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt 14520 ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt 14580 ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg 14640 gcggtcgttc tagatcggag tagaattccc cgatctagta acatagatga caccgcgcgc 14700 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat 14760 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt 14820 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca 14880 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctc 14940 ttagaactcc caccactttt ggaactccca ccacttttga aggtctggca gttgttcttc 15000 aatctccaac aaactgtttc cttcttcaag gtacgcaaaa ccgtcgcaaa acggaacatt 15060 catttcttga ccaaacataa cgtccggaga gttcgtaaac cgatcatccg attcttcacc 15120 gctgccacta ctactagttt tctcttgatg cttttcggct acatttctca gcacctccaa 15180 ttgattgagt agggcctgat tctctttctt tagagactcg gatttagacg caagcgtctc 15240 gtagttatgc tttagcgcgt tatactctg ctcaatctgc ctcgacttg atcgcgcgcg 15300 ttgttctgg aaccatatcg ccacttgacg aggatgaagc ccgagtttat gtgccaactg 15360 gtgtttcatc cttaactcgg gtctcgactg tgtctcaac atgtactcta ggaacttat 15420 ttgtttgtcg gtaatcgtc tccgcccctc gttcggttc tcctgttg tgttacttg 15480 ttgaagac atggatccga gtcgacctgc agagtaca ccaaaca gggtgacact 15540 cgacaaaaga aacagtacca agcaataaa tagcgtag aggcagggct aaaaaaatcc 15600 acatatagct gctgcatatg ccatcatcca agtatatca gatcaaata attataaaac 15660 atacttgttt atataatag ataggtactc aaggttagag catatgaata gatgctgcat 15720 atgccatcat gtatatgcat cagtaaaacc cacatcaca tgtataccta tcctagatcg 15780 atatttccat ccatcttaaa ctcgtaacta tgaacgctgt ctgacac acatacagtt 15840 ccaaaattaa taaatacacc aggtagttg aaacagtatt ctactccgat ctagaacgaa 15900 tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag catctctgta 15960 tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata tttccatcca 16020 tcatcttcaa ttcgtaacta tgaatatgta tggcacacac atacagatcc aaaattaata 16080 aatccaccag gtagtttgaa acagaattcg taatcatggt catagctgtt tcctgtgtga 16140 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 16200 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 16260 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 16320 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 16380 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 16440 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 16500 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 16560 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 16620 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 16680 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 16740 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 16800 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 16860 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 16920 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 16980 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 17040 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 17100 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 17160 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 17220 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 17280 atctaaagta father aacttggtct gacagtttga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 17400 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 17460 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccgggga agctagagta agtagttcgc 17580. cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 17700. ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 17760 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattgga aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 18060 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 18120 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 18180 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 18240 aaaataaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 18300 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 18360 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 18420 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 18480 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 18540 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 18600 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 18660 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 18720 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 18780 ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc tctagata atgagcattg 18840 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag 18900 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac 18960 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg 19020 acaattgagt atttgacaa caggactcta cagttttatc ttttagtgt gcatgtgttc 19080 tcctttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc 19140 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat 19200 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa 19260 tttagatata aatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa 19320 attaaaaaaa ctaaggaaac atttttcttg ttcgagtag ataatgccag cctgttaaac 19380 gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc 19440 gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc 19500 accgttggac ttgctccctt tattttattc tattttatat ctaaattatt aaataaaaaa 19560 actaaaatag agttttagtt ttcttaattt agaggcta 19598 <210> 3 <211> 5470 <212> DNA <213> Artificial Sequence <220> <223> pIND4+HB4 <400> 3 aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc 60 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt 120 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata 180 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta 240 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt 300 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta 360 catccattta gggtttaggg ttaatggttt ttagacta atttttttag tacatctatt 420 ttattctatt ttagcctcta attaagaaa actaaaactc tattttagtt tttttattta 480 ataatttaga tataaatag aataaaataa agtgactaaa attaaacaa atacccttta 540 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt 600 aaacgccgtc gacgagcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 660 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctc gagagtccg 720 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 780 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat 840 tccttccca ccgctccttc gctttccct cctcgcccgc cgtaataaat agacaccccc 900 tccacaccct cttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct 960 cccccaaatc cacccgtcgg cacccgct tcaaggtacg ccgctcgtcc tccccccc 1020 cccctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac 1080 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta 1140 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt 1320 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt 1380 gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg attack tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg fathercatgtt gatgcgggtt ttactgatgc fathercagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc 1620 tagcggag tagactg tttcaaacta cctggtgtat ttattaatt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800 ctattcatat gctctaacct tgagtaccta tctattataa taacaagta tgttttataa ttattttgat cttgatatac ttggatg gcatatgcag cagctatatg tggattttt 1920 tagccctgcc ttcatacgct atttattgc ttggtactgt ttctttgtc gatgctcacc 1980 ctgttgttg gtgttacttc tgcaggtcga cttagagga tccgatccac catgtctctt 2040 storm storm storm gagaaccga aacgaggggc ggagacht taccgaaaa 2100 caataagtt tcctagagta catgtttgag accagtcga gaccgagtt aaggatgaaa 2160 caccagttgg cacataaact cgggcttcat cctcgtcaag tggcgatatg gttccagaac 2220 aaacgcgcgc gatgaagtc gaggcagatt gagggagc ataacgcgct aaagcataac 2280 tacgagacgc ttgcgtctaa atccgagtct ctaaagaaag agaatcaggc cctactcaat 2340 caattggagg tgctgagaaa tgtagccgaa aagcatcaag aggaaactag tagtagtggc 2400 agcggtgaag aatcggatga tcggtttacg aactctccgg acgttatgtt tggtcaagaa 2460 atgaatgttc cgttttgcga cggttttgcg taccttgaag aaggaacag ttgttggag 2520 attgaagac aactgccaga ccttcaaaag tggtggggagt tctaagagct cgaatttccc cgatcgttca aacatttggc aataagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc fathers tgttgats cgttaagcat gtaatas acatgtaatg catgacgtta tttatgat gggtttttat ttagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcggaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attachment cggccaacgc gcggggagag gcggtttgcg 3060 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 3120 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3300 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3360 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3420 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3480 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3540 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3600 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3660 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 3720 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 3780 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 3840 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3900 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3960 atgaagtttt aaatcaatct aaagttata tgagtaaact tggtctgaca gttaccaatg 4020 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4080 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4140 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4200 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4260 ttgttgccgg gaagctagag tagtagttc gccagttaat agtttgcgca acgttgttgc 4320 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4380 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4440 cttcggtcct ccgatcgttg tcaagagtaa gttggccgca gtgttatcac tcatggttat 4500 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4560 tgagtactca accaagtcat tctgagata gtgtatgcgg cgaccgagtt gctcttgccc 4620 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4680 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 4740 gtaacccact cgtgcacca actgatctc agcatcttt acttcacca gcgttctctgg 4800 gtgagcaaaa acaggaggc aaatgccgc aaaaaaggga ataagggcga cacggaatg 4860 ttgaatactc atactctcc tttcaata ttattgaagc atttatcagg gttattgtct 4920 catgagcgga tacatatttg atgtattta gaaaaaaa aaatagggg ttccgcgcac 4980 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 5040 taaaaatagg cgtatcacga ggcccttcg tctcgcgcgt tcggtg acggtgaaaa 5100 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 5160 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 5220 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 5280 atgcgtagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 5340 ggaagggcga tcggtgcggg cctctcgct attack ctggcgaag ggggatgtgc 5400 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 5460 ggccagtgcc 5470 ggccagtgcc 5470 <210> 4 <211> 5496 <212> DNA <213> Artificial Sequence <220> <223> pIND4+BAR <400> 4 aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc 60 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt 1st20 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata 180 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta 240 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt 300 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta 360 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt 420 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta 480 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta 540 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt 600 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 660 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg 720 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 780 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat 840 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc 900 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct 960 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc 1020 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac 1080 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta 1140 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt 1200 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt 1260 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt 1320 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt 1380 gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg attack tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg fathercatgtt gatgcgggtt ttactgatgc fathercagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc 1620 tagcggag tagactg tttcaaacta cctggtgtat ttattaatt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800. 1800 ctattcatat gctctaacct tgagtaccta tctattataa taacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc 1980. ctgttgtttg gtgttacttc tgcaggtcga ctctagagga tccatcgatt aggaagtaac 2040 catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg acatgccggc 2100 ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc gtaccgagcc 2160 gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct atccctggct 2220 cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct ggaaggcacg 2280 caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac 2340 gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa 2400 gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct 2460 cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga 2520 cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg tcctgcccgt 2580 caccgagatc tgatccgtcg acctgcagat cgttcaaaca tttggcaata aagtttctta 2640 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt 2700 aagcatgtaa taatttacat gtaatgcatg acgttattta tgagatgggt ttttatgatt 2760 agagtcccgc aattatacat ttatacgcg atagaaaaaaatagcg cgcaactag 2820 gataaattat cgcgcgcggt gtcatctatg ttactagatc ggaattcgta atcatggtca 2880 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacacat acgagccgga 2940 agcataagt gtaaagcctg gggtgcctaa tgtgagct aactcacatt aattgcgttg 3000 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3060 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgctcctc gctcactgac 3120 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaa ggcggtatata 3180 cggttatcca cagaatcagg ggataacgca ggaagaaca tgtgagcaaa aggccagcaa 3240 aaggccagga accgtaaaa ggccggttg ctggcgtttt tccataggct ccgcccccct 3300 gacgagcatc aaaaatcg acgctcaagt cagaggtggc gaacccgac aggactataa 3360 agataccagg cgttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3420 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3480 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3540 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3600 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3660 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3720 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3780 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3840 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3900 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3960 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4020 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4080 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4140 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4200 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4260 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4320 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4380 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatcccc 4440 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4500 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 4560 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 4620 4680 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 4740 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4800 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 4860 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4920 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 4980 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 5040 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 5100 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 5160 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 5220 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 5280 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 5340 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 5400 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 5460 tcccagtcac gacgttgtaa aacgacggcc agtgcc 5496 <210> 5 <211> 47611 <212> DNA <213> Artificial Sequence <220> <223> Long insert with flanking regions <400> 5 taatggtgag tgtatgcgct tgcgtctgta ttgtgtttaa aaaactcatt ctaagaaact 60 cgtcatacag tgtccaccgt caaggagcca tctagtaggg tgtggatggg catgtgacag 120 gaaccttgtc gtgtgcacgt tgtatgtcgg tcttgttcaa tatgtcgtct gtttcctcgg 180 cttacatgct cttttacgtg tcgtgcaccc aactgatctt cagcatcttt tactttcacc 240 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 300 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 360 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 420 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 480 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 540 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 600 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 660 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 720 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 780 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 840 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 900 tgtaaaacga cggccagtgc caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 960 cccctcttag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1020 tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatatttt aaactttact 1080 ctacgaataa tataatctat agtactacaa tatatcagt gttttagaga atcatataaa 1140 tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1200 ttatcttttt agtgtgcatg tgttctcctt ttttttgca atagcttca cctatataat 1260 acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1320 aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1380 ctatttagt ttttttattt aatatttag fatheraata gaataaata aagtgactaa 1500. 1500. 1500. 1500. 1500. 1500. 1500. 1500. 1500. 1500. 1500 agtagtaat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1680. ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1740 gcaccggcag ctacggggga ttccttttccc accgctcctt cgctttccct tcctcgcccg 1800. ccgtaataa tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcgtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgtaacg tccggagagt tcgtaaaccg atcatccgat tcttcaccgc tgccactact actagttttc tcttgatgct ttcggctac 2160 atttctcagc acctccaatt gattgagtag ggcctgattc tctttcttta gagactcgga 2220 tttagacgca agcgtctcgt agttatgctt tagcgcgtta tactcttgct caatctgcct 2280 cgactttgat cgcgcgtt tgttctggaa catatcgcc acttgacgag gatgaagccc 2340 gagttatgt gccaactggt gtttcatcct taactcgggt ctcgactgtg tctcaaacat 2400 gtactctagg aaacttattt gtttgcggt aaatcgtctc cgcccctcgt ttcggttctt 2460 cctggtggtt gttacttgtt gaagagacat ggatccgggt gtgatcctct atagtcgacc 2520 tgcagaagta acaccaaaca acagggtgag catcgacaaa agaaacagta ccaagcaaat 2580 aatagcgta tgaaggcagg gctaaaaaaa tccacatata gctgctgcat atgccatcat 2640 ccaagtatat caagatcaaa ataattataa aacatacttg tttattataa tagataggta 2700 ctcaaggtta gagcatatga atagatgctg catatgccat catgtatatg catcagtaaa 2760 acccacatca acatgtatac ctatcctaga tcgatatttc catccatctt aaactcgtaa 2820 ctatgaagat gtatgacaca cacatacagt tccaaaatta ataaatacac caggtagttt 2880 gaaacagtat tctactccga tctagaacga atgaacgacc gcccaaccac accacatcat 2940 cacaaccaag cgaacaaaaa gcatctctgt atatgcatca gtaaaacccg catcaacatg 3000 tatacctatc ctagatcgat atttccatcc atcatcttca attcgtaact atgaatatgt 3060 atggcacaca catacagatc caaaattaat aaatccacca ggtagttga aacagaattg 3120 gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 3180 acacaacata cgagccggaa gcataaagtg taagcctgg ggtgcctaat gagtgagcta 3240 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 3300 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 3360 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3420 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 3480 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3540 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3600 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3660 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3720 ggcgcttctct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3780 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3840 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3900 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3960 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4020 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4080 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4140 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4200 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4260 aatctaaagt atatatgagt aaacttggtc tgacagtttt caaatatgta tccgctcatg 4320 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 4380 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 4440 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 4500 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 4560 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 4620 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4680 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 4740 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 4800 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 4860 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 4920 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4980 ttaatagact ggatggaggc ggataagtt gcaggaccac ttctgcgctc ggcccttccg 5040 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt 5100 gcagcactgg ggccagatgg taagccctcc cgtacgtag ttactacac gacggggagt 5160 caggcaacta tggatgaacg aaatagacag atcgctga taggtgccctc actgattaag 5220 cattggtaaa actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 5280 atttttaattt taaaaggatc taggtgaga tccttttttga taatctcatg accaaatcc 5340 cttaacgtga gtttcgttc cactgagcgt cagaccccgt agaagatcg ttcaacatt 5400 tggcaataaa gtttcttaag attgaatcct gttgccggtc tgcgatgat tatcatataa 5460 tttctgttga attacgttaa gcatgtataa atttacatgt aatgcatgac gttatttag 5520 agatgggttt ttatgattag agtccccgcaa ttatacatttt atacgcgat agaaaacaaa 5580 atatagcgcg siactagga taattatcg cgcgcggtgt catctatgtt actagatcgg 5640 atccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg cggacatgcc 5700 ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact tccgtaccga 5760 gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc gctatccctg 5820 gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggc 5880 acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc gccaccagcg 5940 gacgggactg ggctccacgc tctacaccca cctgctgaag tcctggagg cacagggctt 6000 caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc 6060 gctcggatat gccccccgg gcatgctgcg ggcggccggc ttcaagcacg ggaactggca 6120 tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc cggtcctgcc 6180 cgtcaccgag atcgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt 6240 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat 6300 taacatgtaa tgcatgacgt tattatgag atgggtttt atgattagag tcccgcaatt 6360 atacatttaa tacgcgatag aaaacaaaat atacgcgca aactaggata aattatcgcg 6420 cgcggtgtca tctatgttac tagatcggaa ttcgtaatca tggtcatagc tgtttcctgt 6480 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6540 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6600 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6660 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6720 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6780 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6840 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6900 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6960 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7020 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7080 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7140 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7200 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7260 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7320 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7380 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7440 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7500 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7560 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7620 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7680 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7740 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7800 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7860 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7920 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7980 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8040 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8100 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8160 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8220 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8280 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8340 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8400 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8460 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8520 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aaaatagg 8580 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8640 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gttcggtga 8700 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 8760 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 8820 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 8880 aataccgcac agatgcgtaa ggaaaata ccgcatcagg cgccattcgc cattcaggct 8940 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9000 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9060 ttgtaaaacg acggccagtg caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 9120 ccctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 9180 tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatatttt aaactttact 9240 ctacgaataa tataatctat agtactacaa tatatcagt gttttagaga atcatataaa 9300 tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat tttagcctct aattaagaa aactaaact ctatttagt ttttttattt aatatttag aataataata aataaaata aagtgactaa aaattaaca aatacccttt aagaaattaa aaaaaactaaag gaacatttt tcttgtttcg agtagtaat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 9780. ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 9840 ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 9900 gcaccggcag ctacggggga ttccttttccc accgctcctt cgctttccct tcctcgcccg 9960 ccgtaataa tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac 10080 gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg 10140 gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga 10200 tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct 10260 aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg 10320 atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat 10380 ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg 10440 ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa 10500 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta 10560 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt 10620 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt 10680 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta 10740 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat 10800 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat 10860 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata 10920 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca 10980 gcagctatat gtggatttt ttagccctgc cttcatacgc tatttatttg cttggtactg 11040 tttctttgt cgatgctcac cctgttgttt ggtgttactt ctgcagggat ccatgagccc 11100 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 11160 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 11220 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 11280 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 11340 cgactggacg gccgagtcga ccgtgtacgt ctcccccgc caccagcgga cgggactggg 11400 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 11520 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 11580 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 11640 cgatcgttca aacatttggc aataagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc father tgttgat cgttaagcat gtaata acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatctgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 12180 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 12240 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa gatcttacc gctgttgaga 12300 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 12360 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 12420 acacggaaat gttgaatact catactcttc cttttcaat attattgaag catttatcag 12480 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 12540 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 12600 acattaacct ataaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 12660 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 12720 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 12780 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 12840 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 12900 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 12960 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 13020 tgtaaaacga cggccagtgc caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 13080 cccctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 13140 tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatatttt aaactttact 13200 ctacgaataa tataatctat agtactacaa tatatcagt gttttagaga atcatataaa 13260 tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 13320 ttatcttttt agtgtgcatg tgttctcctt ttttttgca atagcttca cctatataat 13380 acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 13440 aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 13500 ctattttagt ttttttatt aataatttag atataaaata gaataaaata aagtgactaa 13560 aaattaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 13620 agtagataat gccagcctgt taaacgccgt ttgttgaata gaatacaaac cctagaaaaa 13680 caaggcacat ctcggcgccg ccaatagaag gcagaaaccc tagccgtcat ttttcttgaa 13740 gaatatagag cgacgtcgat aggaagatac ttaaatgttg aatccaagca cctagaataa 13800 tgagttagat tagaagaaaa ctatcaactt cgaaatcaag aagaaatgtt ttattctgtt 13860 gcataaaact ttcgcgtgga gaagctctgt tcagagcccg ggtagccact gggcacgtct 13920 cctagatact ggactcatcg gacgctctcc tcgagccatt aatatggtc gtcctgtaga 13980 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga 14040 aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc 14100 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcggggaagc 14160 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 14220 aagctcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg 14280 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat 14340 aacgtgctga tggtgcacga ccacgcattg atggactgga ttggggccaa ctcctaccgt 14400 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg 14460 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg 14520 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg 14580 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg 14640 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca 14700 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg 14760 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt 14820 tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa 14880 gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg 14940 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt 15000 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag 15060 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag 15120 aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg ctcgctcagt gtgtgtgact 15180 ctgctggtgc tggggaatgg agctcggggc accgtatttg tagcgcgcgc gccgctgtac 15240 gtggcgccct ggcgcggcta cgtggagggg agctgcgtgc cagaggggag gcgggccgcc 15300 acgtcgcacg gccgtgctta tcgggccatc ggcgaggtgc gtgccagctt gggcgtcgcg 15360 tgtcgtggag cgctcctgct cgcggcggag ctggttgagt ggattgcgat gcggtgcttg 15420 gtacagagct cgatggacaa ctcgaggaga gcgtccgatg agtccagtat ctaggagacg 15480 tgcccagtgg ctacccgggc tctgaacaga gcttctccac gcgaaagttt tatgcaacag 15540 aataaaacat ttcttcttga tttcgaagtt gatagttttc ttctaatcta actcattatt 15600 ctaggtgctt ggattcaaca tttaagtatc ttcctatcga cgtcgctcta tattcttcaa 15660 gaaaaatgac ggctagggtt tctgccttct attggtcatt acgcgcagaa aaaaaggatc 15720 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 15780 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 15840 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttttac 15900 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 15960 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 16020 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 16080 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 16140 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 16200 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 16260 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 16320 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 16380 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 16440 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 16500 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 16560 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 16620 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 16680 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 16740 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 16800 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaaaat aggggttccg 16860 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 16920 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 16980 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 17040 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 17100 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 17160 cacagatgcg taaggagaaa atacgcatc aggcgccatt cgcattcag gctgcgcaac 17220 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaagggga 17280 tgtgctgcaa ggcgattaag ttgggttaacg ccagggtttt cccagtcacg acgttgtaaa 17340 acgacggcca gtgccaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct 17400 ctagagataa tgagcattgc atgtctaagt tataaaaaaat taccacatat tttttttgtc 17460 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga 17520 samaataat ctatagtact acaatgtacg ccgctcgtcc tcccccccccc cccctctcta 17580 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat 17640 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg 17700. acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc 17820. atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg 17880. tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt 17940 tctagatcgg agtagtc tgtttcaaac tacctggtgg attack tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaga tgatggatgg aaatatcgat ctaggatagg fathercatgtt gatgcgggtt ttactgatgc fathercagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc tagatcggag 18180 tagaatactg tttcaaacta cctggtgtat ttattaatt tggaactgta tgtgtgtgtc attackcttc atagttacga gtttaagatg gatggaaata tcgatctagg attacktac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat ctattcatat 18360 gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat 18420 cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc 18480 ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg 18540 gtgttacttc tgcagtcgac tcgtagagga tccatcccga ttaggaaagt agagcatgag 18600 cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg 18660 caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga 18720 accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc 18780 cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggc ccctggaagg cacgcaacgc 18840 ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc ggacgggact 18900 gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt 18960 ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata 19020 tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg 19080 tttctggcag ctggacttca gcctgccggt accgccccgt ccggtcctgc ccgtcaccga 19140 gatcatatga atagatgctg catatgccat catgtatatg catcagtaaa acccacatca 19200 acatgtatac ctatcctaga tcgatatttc catccatctt aaactcgtaa ctatgaagat 19260 gtatgacaca cacatacagt tccaaaattt ataaatacac caggtagtttt gaaacagtat 19320 tctactccga tctagaacga atgaacgacc gcccaaccac accacatcat cacaaccaag 19380 cgaacaaaaa gcatctctgt atatgcatca gtaaaacccg catcaacatg tatacctatc 19440 ctagatcgat atttccatcc atcatcttca attcgtaact atgaatatgt atggcacaca 19500 catacagatc caaaattaat aaatccacca ggtagtttga aacagaattc tactccgatc 19560 tagaacgacc gcccaaccag accacatcat cacaaccaag acaaaaaaaa gcatgaaaag 19620 atgacccgac aaacaagtgc acggcatata ttgaaataaa ggaaaagggc aaaccaaacc 19680 ctatgcaacg aaacaaaaaa aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca 19740 tcccaggatt ccccaaagag aaacactggc aagttagcaa tcagaacgtg tctgacgtac 19800 aggtcgcatc cgtgtacgaa cgctagcagc acggatctaa cacaaacacg gatctaacac 19860 aaacatgaac agaagtagaa ctaccgggcc ctaaccatgg accggaacgc cgatctagag 19920 aaggtagaga gggggggggg gggaggacga gcggcgtacg ccgacgggtg gatttggggg 19980 agatctggtt gtgtgtgtgt gcgctccgaa caacacgagg ttggggaaag agggtgtgga 20040 gggggtgtct atttattacg gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga 20100 atcccccgta gctgccggtg ccgtgagagg aggaggaggc cgcctgccgt gccggctcac 20160 gtctgccgct ccgccacgca atttctggat gccgacagcg gagcaagtcc aacggtggag 20220 cggaactctc gagaggggtc cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt 20280 ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt tagactcgtc gacggcgttt 20340 aacaggctgg cattatctac tcgaaacaag aaaaatgttt ccttagtttt tttaatttct 20400 taaagggtat ttgtttaatt tttagtcact ttattttatt ctatttatta tctaaattat 20460 taaaaaaaaaaaaata gagttttagtt tttctttatt tagaggcta aatagaata 20520 atagatgta ctaaaaaaat tagtcttaa aaaccattaa ccctaaaccc taatggatg 20580 tactataaa atggatgag tattatatag gtgaagctat ttgcaaaaaaaggagaac 20640 acatgcacac tAAaagata aactgtaga gtcctgttgt caaatactc aattgtcctt 20700 tagaccatgt ctaactgttc atttatatga ttctctaaaa cactgatatt attgtagtac 20760 tatagatta attattcgta gagtaagtt taatatatg tataagat gataactgc 20820 acttcaaaca agtgtgacaaaaaaatatg tggtatttt ttataactta gatagcaat 20880 gctcattatc tctagagggg ggcacgaccg gtcacgctg cactgcaggc atgcaagctt 20940 gcactggccg tcgttttaca acgtcgtgac tgggaaacc ctggcgttac ccaacttaat 21000 cgccttgcag cacaccccc ttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 21060 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 21120 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 21180 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 21240 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 21300 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 21360 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 21420 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 21480 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 21540 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 21600 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 21660 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 21720 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 21780 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 21840 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 21900 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 21960 ggaccgaagg agctaaccgc ttttttgcac aacatgggg atcatgtaac tcgccttgat 22020 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 22080 gtagcaatgg caacaacgtt gcgcaacta ttaactggcg aactacttac tctagcttcc 22140 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 22200 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 22260 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 22320 acggggagatc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 22380 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 22440 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatcctttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 22620. ccgctaccag cggtggtttg tttgccggat caagagctac caactcttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga tagtcgtgt cttaccgggt tggactcaag acgatagtta 22860 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatcttata gtcctgtcgg gtttcgccac 23100 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 23160 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 23220 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 23280 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 23340 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcatttatg cagctggcac 23400 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caatttatgt gagttagctc 23460 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt aagcttgcat 23520 gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc 23580 taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc 23640 tatctttata catatattta aactttactc tacgaataat ataatctata gtactacaat 23700 aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt 23760 gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt 23820 ttttttgcaa atagcttcac ctatatata cttcatccat tttattagta catccattta 23880 gggtttaggg ttaatggttt ttagacta atttttttag tacatctatt ttattctatt 23940 ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga 24000 tataaatag aataaaataa agtgactaaa attaaaaa atacccttta agaaattaaa 24060 aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc 24120 gacgagcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca 24180 gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt 24240 ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc 24300 acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca 24360 ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacacccga actgtatgtg 24420 tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag 24480 gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat 24540 tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat 24600 tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga tttttttagc 24660 cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt 24720 tgtttggtgt tacttctgca gggatccatg tctcttcaac aagtaacaac caccaggaag 24780 aaccgaaacg aggggcggag acgatttacc gacaaacaaa taagtttcct agagtacatg 24840 tttgagacac agtcgagacc cgagttaagg atgaaacacc agttggcaca taaactcggg 24900 cttcatcctc gtcaagtggc gatatggttc cagaacaaac gcgcgcgatc aaagtcgagg 24960 cagattgagc aagagtataa cgcgctaaag cataactacg agacgcttgc gtctaaatcc 25020 gagtctctaa agaaagagaa tcaggcccta ctcaatcaat tggaggtgct gagaaatgta 25080 gccgaaaagc atcaagagaa aactagtagt agtggcagcg gtgaagaatc ggatgatcgg 25140 tttacgaact ctccggacgt tatgtttggt caagaaatga atgttccgtt ttgcgacggt 25200 tttgcgtacc ttgaagaagg aaacagtttg ttggagattg aagaacaact gccagacctt caaaagtggt gggagttcga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta father tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg 25500. 25500. 25500. 25500. 25500. 25500. 25500. 25500. 25500. 25500 tcgcgcgcgg tgtcatctat gttactagat cggaattcgt aatcatggtc atagctgttt 25560 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 25800. tcggtcgttc ggctgcggcg agcggtatca gctcactcaa agcggtaat acggttatcc 25860. tcggtcgttc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 25980 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 26040 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 26100 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 26160 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 26220 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 26280 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 26340 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 26400 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 26460 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 26520 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 26580 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 26640 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 26700 tctgacagtt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 26760 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 26820 tactctagct tcccggcaac attaataga ctggatggag gcggataaag ttgcaggacc 26880 acttctgcgc tcggcccttc cggctggctg gttattgct gataaatctg gagccggtga 26940 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 27000 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaatagac agatcgctga 27060 gatagaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 27120 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 27180 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 27240 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 27300 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 27360 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 27420 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 27480 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 27540 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 27600 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 27660 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 27720 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 27780 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 27840 gacgctgggc cgccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 27900 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 27960 gtgtcacgca gatcctatga aatttggtcg atgattgtct tttatggatc cgttcagacc 28020 aagtcttcgt gtgtctatgt tatcgtgcct ttaaattgaa tcctttcgat ttatgcttct 28080 ttttgtcagc ggtggttgat cttctgatgt gctggttaca aagggtctta gcataacaat 28140 tttccaattg tctactacaa caagtttgt ctaacttcga tgaagcctag gtgatgacga 28200 tttcaacttg cttcggtgct tgtaactgtt attagatggt ctacggatct aaatgtaact 28260 tctattattt gttgtgtttt ttgtattatt atgatggatg atgaatagat taaaagatcc 28320 cgcaaataaa accttaaatg ataaatgtca cacgtcacgt gcatggcact ccggccctaa 28380 tgcctttgt agctattcta gtaacgcaag gatagcaaat ttccgtgaca cgacgcaagt 28440 ttttgttaaa aataaaattaa agcaagaaaa ttgtcatact tgctaacgaa aattgccatc 28500 cttgcctatt catcatccat cataataata caaaaaacac aacaaataat agaagttaca 28560 tttagatccg tagaccatct aataacagtt acaagcaccg aagcaagttg gtgccgtctg 28620 cttcgcttgg cccgacgcga cgctgctggt tcgctggttg gtgtccgtta gactcgtcga 28680 cggcgtttaa caggctggca ttatctactc gaaaagaaa aaatgtttcc ttagtttttt 28740 taatttctta aagggtattt gtttaatttt tagtcacttt attttatct attttattc 28800 taaattatta aataaaaaaa ctaaaataga gttttagttt tcttaattta gaggctaaaa 28860 tagaataaaa tagatgtact aaaaactcaa ttgtccttta gaccatgtct aactgttcat 28920 ttatatgatt ctctaaggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 28980 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 29040 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 29100 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 29160 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 29220 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 29280 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 29340 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 29400 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 29460 tacattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 29520 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 29580 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 29640 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 29700 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 29760 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 29820 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 29880 aagcggttag ctccttcggt cctccgaata ccgcgccaca tagcagaact ttaaaagtgc 29940 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 30000 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 30060 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggg ataaggggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatattg aagatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc 30360 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc 30420. agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg 30480 30540. gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtat ggatgaagta ttatataggt gaagctattt gcaaaaaaaa aggagacac atgcacacta aaaagataa actgtaggt cctgttgtca aaatactcaa ttgtccttta gaccatgtct aactgttcat ttatgatt ctctaaaaca ctgatattat tgtagtacta tattgattat tattcgtaga gtaagttta atatatgta 30780 taaagataga taaactgcac ttcaacaag tgtgacaaa aaaatatgtg gtaatttttt 30840 ataacttaga catgcaatgc tcattactc tagagagggg cacgaccggg tcacgctgca 30900 ctgcaggcat gcaagcttct gcagaagtaa caccaaaca cagggtgagc atcgacaaaa 30960 gaaaaaaaat ccacatatag 31020 ctgctgcata tgccatcatc caagtatatc aagatcaaa tattattaaa acatacttgt 31080 ttattataat agataggtac tcaggttag agcatatgaa tagatgctgc atatgccatc 31140 atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat cgatatttcc 31200 atccatctta aactcgtaac tatgagatg tatgacacac acatacagtt ccaaaattaa 31260 taaatacacc aggtagttg aaacagtatt ctactccgat ctagaacgaa tgaacgaccg 31320 cccaaccaca ccacatcatc acaaccaagc gaaaaaag catctgta tatgcatcag 31380 taaaacccgc atcacatgt atacctacc tegatcgata ttcatcca tcatctca 31440 ttcgtaacta tgaatatgta tggcacac atacagatcc aaaattaata aatccaccag 31500 gtagtttgaa acagaatttct actccgatct agaacgaccg cccaccaga ccacatcatc 31560 aaaaaaaaag catgaaaga tgaccgaca aacaagtgca cggcatatat 31620 tgaaataag gaaaagggca aaccaaaccc tatgcaacga aaaaaaaaa atcatgaat 31680 cgatcccgtc tgcggaacgg ctagagccat cccaggattc cccaagaga aacactggca 31740 agttagcaat cagaacgtgt ctgacgtaca ggtcgcatcc gtgtacgaac gctagcagca 31800 cggatctaac acaacacgg atctacaca aacatgaaca gaagtagaac taccgggccc 31860 taaccatgga ccggaacgcc gattagaga aggtagagag gggggggggg ggaggacgag 31920 cggcgtacct tgaagcggag gtgccgacgg gtggattgg gggagatctg gttgtgtgtg 31980 tgtgcgctcc gaacacacg aggttgggga agagggtgt ggaggggtg tctatttatt 32040 acggcggggcg aggaaggaa aggaggag cggtgggaa ggaatcccc gtagctgccg 32100 gtgccgtgag aggagga ggccgcctgc cgtgccggct cacgctgcc gctccgccac 32160 gcaatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagagggg 32220 gtccagaggc agcgacagag atgccgtgcc gtctgctcg cttggcccga cgcgacgctg 32280 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg ttacaggc tggcattatc 32340 tactcgaaac aagaaaaatg ttccttagt ttttaatt tcttaaggg tatttttttta 32400 atttttagtc actttatttt attctatttt atatctaaat tattaataa aaaaactaaa 32460 atagagtttt agttttctta atttagaggc taaaatagaa taaatagat gtactaaaaa 32520 aattagtcta taaaaaccat taaccctaa ccctaaatgg atgtactaat aaatggatg 32580 aagtattata taggtgaagc tatttgcaa aaaaaagg aacacatgca cactaaaag 32640 aaaactgt agagtcctgt tgtcaaata ctcattgtc ctttagacca tgtctaactg 32700 ttcatttata tgattctcta aacactgat attattgtag tactatagat tatattattc 32760 gtagagtaaa gttaataat atgtataaag atagataac tgcactcaa acagtgtga 32820 caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt atctctagag 32880 aggggcacga ccgggtcacg ctgcactgca ggcatgcaag cttgcactgg ccgtcgtttt 32940 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 33000 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 33060 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg 33120 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 33180 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 33240 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 33300 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 33360 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 33420 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 33480 accctgataa atgcttcaat aatattgaaa aaagaagagt atgagtattc aacatttccg 33540 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 33600 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 33660 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 33720 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 33780 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 33840 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 33900 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 33960 cgctttttttg caacacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 34020 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 34080 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 34140 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 34200 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 34260 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 34320 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 34380 actgtcagac caagtttact entatatact ttagattgat ttaaaacttc atttttaatt 34440 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 34500 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 34560 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 34620 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 34680 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 34740 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 34800 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 34860 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 34920 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 34980 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 35040 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 35100 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 35160 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 35220 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 35280 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 35340 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 35400 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 35460 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 35520 tcacacagga aacagctatg accatgatta cgaattccga tcgttcaaac atttggcaat 35580 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt 35640 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg 35700 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc 35760 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgatctcggt 35820 gacgggcagg accggacggg gcggtaccgg caggctgaag tccagctgcc agaaacccac 35880 gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc 35940 gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct 36000 cttgaagccc tgtgcctcca gggacttcag caggtgggtg tagagcgtgg agcccagtcc 36060 cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt 36120 gcgtgccttc caggggcccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac 36180 gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg 36240 cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac 36300 cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat 36360 ggatccaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc tctagata 36420 atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt 36480 tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa 36540 tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat 36600 ggtctaaagg acaattgagt atttgacaa caggactcta cagttttatc tttttagtgt 36660 gcatgtgttc tcctttttt ttgcaaatag cttcacctat ataatacttc atccatttta 36720 tttacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca 36780 tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt 36840 tatttaataa tttagatata aatagaata aaataaagtg actaaaaatt aaacaaatac 36900 cctttaagaa attaaaaaaa ctaaggaaac atttttcttg ttcgagtag ataatgccag 36960 cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt 37020 cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga 37080 gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg 37140 gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg 37200 ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac 37260 accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc 37320 agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc 37380 cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag 37440 ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg 37500 ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt 37560 ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt 37620 ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt 37680 gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt 37740 ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc tggtggattt 37800 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat 37860 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 37920 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat 37980 tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga 38040 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga 38100 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg 38160 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt 38220 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga 38280 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg 38340 ctcaccctgt tgtttggtgt tacttctgca gggatccatg agcccagaac gacgcccggc 38400 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta 38460 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga 38520 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt 38580 cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact ggacggccga 38640 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac 38700 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct 38760 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct 38820 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt 38880 cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgat ccgtcgacct 38940 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 39000 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 39060 tgcatgacgt tattatgag atgggtttt atgattag tcccgcaatt atacatttaa 39120 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 39180 tctatgttac tagatcggaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 39240 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 39300 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 39360 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 39420 cgtattgggc gctcttcgc ttctcgctc actgactcgc tgcgctcggt cgtcggctg 39480 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 39540 aacgcaggaa agaacatgtg agcaaaagc cagcaaaagg ccaggaaccg taaaaggcc 39600 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 39660 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 39720 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 39780 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 39840 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 39900 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 39960 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 40020 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 40080 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 40140 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 40200 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 40260 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 40320 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 40380 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 40440 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 40500 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 40560 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 40620 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 40680 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 40740 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 40800 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 40860 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 40920 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 40980 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 41040 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 41100 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 41160 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 41220 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 41280 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 41340 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 41400 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 41460 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 41520 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 41580 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 41640 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 41700 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 41760 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 41820 acggccagtg caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc cccctcttag 41880 agataatgag cattgcatgt ctaagttata aaaaattacc acatatttt ttgtcacac 41940 ttgtttgaag tgcagtttat ctatctttat acatatttt aaactttact ctacgaataa 42000 ataatctat agtactaca tatactagt gttttagaga atcatataa tgacagtta 42060 gabatggtct aaaggacaat tgagtatttt gandacagga ctctacagtt ttatcttttt 42120 agtgtgcatg tgttctcctt ttttttgca atagcttca cctatataat acttcatcca 42180 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta 42240 gtacatctat tttattctat aaattaagaa actaaact ctattttagt 42300 tttttatttt attaatttag attaaaata gataaata agtgactaa aattaaca 42360 atacccttt aagaattta aaaaactaag gaacatttt tcttgtttcg agtagattaat 42420 gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa cccagcagcgt 42480 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct 42540 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg 42600 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag 42660 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa 42720 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca 42780 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc 42840 ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc 42900 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc 42960 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag 43020 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca 43080 tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat 43140 gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat 43200 gtggtctggt tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg 43260 gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag 43320 atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 43380 catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg 43440 ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt 43500 ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat 43560 atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc 43620 atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt 43680 atgtttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat 43740 gtggatttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttctttgtgt 43800 cgatgctcac cctgttgttt ggtgttactt ctgcaggtcg actctagagg atccatcgat 43860 taggaagtaa ccatgagccc agaacgacgc ccggccgaca tccgccgtgc caccgaggcg 43920 gacatgccgg cggtctgcac catcgtcaac cactacatcg agacaagcac ggtcaacttc 43980 cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc 44040 tatccctggc tcgtcgccga ggtggacggc gaggtcgccg gcatcgccta cgcgggccc 44100 tggaaggcac gcaacgccta cgactggacg gccgagtcga ccgtgtacgt ctcccccgc 44160 caccagcgga cgggactggg ctccacgctc tacacccacc tgctgaagtc cctggaggca 44220 cagggcttca agagcgtggt cgctgtcatc gggctgccca acgacccgag cgtgcgcatg 44280 cacgaggcgc tcggatatgc ccccgcggc atgctgcggg cggccggctt caagcacggg 44340 aactggcatg acgtgggttt ctggcagctg gacttcagcc tgccggtacc gccccgtccg 44400 gtcctgcccg tcaccgagat ctgatccgtc gacctgcaga tcgttcaaac atttggcaat 44460 aaagttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt 44520 tgaattacgt taagcatgta atattaca tgtaatgcat gacgttattt atgagatggg 44580 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc 44640 gcgcaaacta ggataatta tcgcgcgcgg tgtcatctat gttactagat cggaattcgt 44700 aatcatggtc atagctgttt cctgtgtgaa attgttacc gctcacaatt ccacacaca 44760 tacgagccgg aagcataag tgtaagcct ggggtgccta atgagtgagc taactcacat 44820 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 44880 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggcgctct tccgcttcct 44940 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 45000 aggcggtaat acggttatcc acagaatcag gggataacgc aggaagaac atgtgagca 45060 aaggccagca aaggccagg aaccgtaaaa aggccgcgtt gctggcgtttt ttccataggc 45120 tccgcccccc tgacgagcat cacaaaatc gacgctcaag tcagaggtgg cgaaacccga 45180 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 45240 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 45300 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 45360 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 45420 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 45480 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 45540 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 45600 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 45660 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 45720 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 45780 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 45840 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 45900 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 45960 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 46020 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 46080 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 46140 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 46200 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 46260 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 46320 gaagtaagtt ggccgcagtg tttcactca tggttatggc agcactgcat aattctctta 46380 ctgtcatgcc atccgtaaga tgctttctg tgactggtga gtactcaacc aagtcattct 46440 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 46500 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 46560 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 46620 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 46680 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 46740 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 46800 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 46860 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 46920 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 46980 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 47040 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 47100 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 47160 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 47220 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 47280 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcgtcg actctagagg 47340 tccctcgacc atggctgtaa ggtcgccgaa tacaagggca tgggtggcga ggcctaagag 47400 gagtataatt tcttagacat ttgtcaatta taaacttctt ggtaaaagag catgtaagcc 47460 gaggaaacag acgacatatt gaacaagacc gacatacaac gtgcacacga caaggttcct 47520 gtcacatgcc catccacacc ctactagatg gctccttgac ggtggacact gtatgacgag 47580 tttcttagaa tgagtttttt aaacacaata c 47611 <210> 6 <211> 20418 <212> DNA <213> Artificial sequence <220> <223> Short insert with flanking regions <400> 6 gagcgcgctt tcctatttag gatccaaatt ggcactgata ctaaactgct tagacgaatc 60 gcctggagta aaatcaacaa gctcggtttc agcagccgac ttaaacttca tcgccgggtc 120 atgctcggtg gttggcttct ttagagacgt catatccgcc aggtcaacat tattttgata 180 aaaattcatc tcctctgccg cacaaacaga ctcagcataa gccgcatcgc cttcttcaca 240 ttctagggct acctttcgac tcccattgag ggagtcccgg attagggggt gtttggaaca 300 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 360 ctctagcttc ccggtaacaa ttaatagact ggatggaggc ggataaagtt gtaggaccac 420 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggactct 480 aggcttcccg ggcaacaaat taattagact ggatggaggg ataaagtttg cagaccactt 540 tctgcgctg gccttccgc tgggctgttc tatgcccgct gaaatcacca gtctctctct 600 acaaatctat ctctcttat aataatgtgt gagtagttcc cagataaggg aattagggtt 660 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt 720 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 780 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 840 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 900 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 960 acgttgtaaa acgacggcca gtgcaagctt gcatgcctgc agtgcagcgt gacccggtcg 1020 tgcccctctc tagataat gagcattgca tgtctaagtt aaaaaatt accacatatt 1080 ttttttgtca cacttgtttg aagtgcagtt tatctatct tatacatata tttaacttt 1140 acttacgaa tatataatc tatagtacta cataatatc agtgttttag agaatcatat 1200 aaatgaacag ttagacatgg tctaaggac aattgagtat ttgacaca ggactctaca 1260 gttttatctt tttagtgtgc atgtgttctc ctttttttt gcaatagct tcacctatat 1320 atacttcat ccattttatt agtacatcca tttaggtttt agggttaatg gttttattag 1380 actaattttt ttagtacatc tattttattc tattttagcc tctaaattta gaaactaaa 1440 actctatttt agtttttt tttaatatt tagatataa atagaataa aaagtgac 1500 taaaaattta acaataccc tttaagaat taaaaaact aaggaacat ttttcttgtt 1560 tcgagtagat atgccagcc tgttaaacgc cgtcgacgag tctaacggac accaccagc 1620 gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc 1680 tctggacccc tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag 1740 aaattgcgtg gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc 1800 acggcaccgg cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc 1860 ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga 1920 gcgcacacac acacaaccag atctccccca aatccacccg tcggcgtacg ccgctcgtcc 1980 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg 2040 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct 2100 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt 2160 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat 2220 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg 2280 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttcct gtttttgctc 2340 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 2400 acatcgaact ggatctcaac agcggtaaga tccttgagag ttcgcccc gagaacgtt 2460 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 2520 ccggggcaaga gcaactcggt cgccgcatac actattctca gatgacttg gttgagtact 2580 caccagtcac agaaaagcat cttacggatg gcatgacagt agagaatta tgcagtgctg 2640 ccataccat gagtgataac actgcggcca acttactct gandacgatc ggaggaccga 2700 aggagctaac cgctttttg cacacatgg gggatcatgt aactcgcctt gatcgttggg 2760 aaccggagct gatgaagcc ataccaacg acgagcgtga caccacgatg cctgtagcaa 2820 tggcaacaac gttgcgcaaa ctattaactg ttattgccgg gaaagtgta cgtatcaccg 2880 tttgtgtgaa caacgaactg aactggcaga ctatcccctg cacaagtaac accaacac 2940 agggtgagca tcgacaaag aaacagtacc aacaataa atagcgtag aaggcagggc 3000 taaaaaaatc cacatatagc tgctgcatat gccatcatcc aagtatatca agatcaaat 3060 aattaaaa catacttgtt tattaata gataggtact caagttaga gcatatgaat 3120 agatgctgca tatgccatca tgtatatgca tcagtaaac ccacatcaac atgtatacct 3180 atcctagatc gatatttcca tccatcttaa actcgtaact atgagatgt atgacacaca 3240 catacagttc caaattaat aaatacacca ggtagtttga aacagtattc tactccgatc 3300 tagaacgaat gaaaccgc spaccacac spaccaagcg aaaaaagc 3360 atctctgtat atgcatcagt aaaacccgca tcacatgta tacctacct agaagtacta 3420 taacttat tattcgtaga gtaagtttta tattatagta taagataga taactgcac 3480 ttcaacaag tgtgacaaaa aaatatgtg gtaattttt ataacttaga catgcaatgc 3540 tcattatctc taggagggg cacgaccgggg tcacgctgca ctgcaggcat gcaagcttgg 3600 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 3660 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 3720 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc 3780 tacgcatct gtgcggtatt tcacaccgca tattgtgcac tccagtaca atctgctg 3840 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 3900 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 3960 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4020 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4080 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattac caatgcttaa 4140 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4200 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4260 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4320 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4380 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4440 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4500 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 4560 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 4620 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 4680 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 4740 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 4800 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 4860 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 4920 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 4980 tactcatact cttccttttt caatattatt gaagcattta tcaggggttat tgtctcatga 5040 gcggatacat atttgaaaac tgtcagacca agtttactca tatatacttt agattgattt 5100 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 5160 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5220 aggatcttct tgagatcctt tttttctgcg cgtaagccaa tagaaggcag aaaccctagc 5280 cgtcattttt cttgaagaat atagagcgac gtcgatagga agatacttaa atgttgaatc 5340 caagcaccta gaataatgag ttagattaga agaaaactat caacttcgaa atcaagaaga 5400 aatgttttat tctgttgcat aaaactttcg cgtggagaag ctctgttcag agcccgggta 5460 gccactgggc acgtctccta gatactggac tcatcggacg ctctcctcga gttgtccatc 5520 gagctctgta ccaagcaccg catcgcaatc cactcaacca gctccgccgc gagcaggagc 5580 gctccacgac acgcgacgcc caagctggca cgcacctcgc cgatggcccg ataagcacgg 5640 ccgtgcgacg tggcggcccg cctcccctct ggcacgcagc tcccctccac gtagccgcgc 5700 cagggcgcca cgtacagcgg cgcgcgcgct acaaatacgg tgccccgagc tccattcccc 5760 agcaccagca gagtcacaca cactgagcga gctaaaccat caggaagtga tggagcatca 5820 gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt 5880 acgtatcacc gtttgtgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac 5940 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt 6000 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca 6060 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg 6120 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg 6180 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct 6240 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg 6300 tggcaaagga ttcgataacg tgctgttacc aatgcttaat cagtgaggca cctatctcag 6360 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6420 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6480 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6540 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6600 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6660 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6720 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6780 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6840 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6900 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6960 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7020 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7080 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7140 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7200 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7260 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7320 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 7380 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 7440 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 7500 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 7560 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 7620 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctct 7680 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 7740 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgcaagcttg catgcctgca 7800 gtgcagcgtg acccggtcgt gccctctct agagataatg agcattgcat gtctaagtta 7860 taaaaatta ccacatattt ttttgtcac acttgtttga agtgcagttt atctatcttt 7920 atacatatat ttaaacttta ctctacgaat aataatct atagtactac aataatatca 7980 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt 8040 ttgacaacag gactctacag ttttatcttt tgtgtgca tgtgttctcc ttttttttg 8100 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta 8160 gggttaatgg ttttataga ctaattttt tagtacatct attttattct attttagcct 8220 ctaaattaag aaaactaaaa ctctatttta gttttttatta tattaattt agataaaa 8280 Tagaaaaa taagtgact aaaaaaaaaccct ttagaattt aaaaaaacta 8340 aggaaacatt tttctgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt 8400 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca 8460 cggcatctct gtctgaatag atacaaacc ctagaaaaac aaggcacatc tcggcgccgc 8520 caatagaagg cagaaaccct agccgtcatt ttcttgaag atatagagc gacgtcgata 8580 ggaagatact taatgttga atccaagcac ctagaataat gagttagatt agagaaaac 8640 tatcaacttc gaatcaaga agaatgttt tattctgttg cataaaactt tcgcgtggag 8700 aagctctgtt cagagcccgg gtagccactg ggcacgctc ctagatactg gactcatcgg 8760 acgctctcct cgagttgtcc atcgagctct gtaccaagca ccgcatcgca atccactca 8820 ccagctccgc cgcgagcagg agcgctccac ccacgcgac gcccaagctg gcacgcacct 8880 cgccgatggc ccgataagca cggccgtgcg acgtggcggc ccgcctcccc tctggcacgc 8940 agctcccctc cacgtagccg cgccagggcg ccacgtacag cggcgcgcgc gctacaaata 9000 cggtgccccg agctccattc cccagcacca gcagagtcac acacactgag cgagctaaac 9060 catggtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca ctttgccgta 9120 atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt tcggtataaa 9180 gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat cggcgaactg 9240 atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc tttcccacca 9300 acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc cgtcgagttt 9360 tttgatttca cgggttgggg tttctacagg acgctattaa ttgttgccgg gaagctagag 9420 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 9480 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 9540 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 9600 tcagaagtaa gttggccgca gtgttatcac tcatggttca ctgtcagacc aagtttactc 9660 atatatactt tagattgatt taaaacttca ttttaattt aaaaggatct aggtgaagat 9720 cctttttgat aatctcatga ccaaaatccc ttaacgtgag tttcgttcc actgagcgtc 9780 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 9840 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 9900 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 9960 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 10020 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 10080 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 10140 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 10200 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 10260 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 10320 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 10380 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 10440 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 10500 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 10560 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 10620 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 10680 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 10740 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 10800 ccatgattac gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta 10860 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat 10920 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac 10980 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa 11040 gaaactttat tgccaaatgt ttgaacgatc tgcaggtcga cggatcagat ctcggtgacg 11100 ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca 11160 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc 11220 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg 11280 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc 11340 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt 11400 gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc 11460 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc 11520 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc 11580 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatggat 11640 ccagctccgc cgcgagcagg agcgctccac gacacgcgac gcccaagctg gcacgcacct 11700 cgccgatggc ccgataaggc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 11760 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 11820 cagttacctt cggaaaaga gttggtagct cttgatccgg caacaaacc accgctgta 11880 gcggtggttt tttgtttgc aagcagcaga ttacgcgcag aaaaaagga tctcaagaag 11940 atcctttgat ctttctacg gggtctgacg ctcagtggaa cgaaaacca cgttaaggga 12000 ttttggtcat gagattatca aaaggatct tcacctagat ccttttaaat taaaaatgaa 12060 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 12120 tcagtgaggc acctactca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 12180 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 12240 taccgcgaga cccacgctca ccggctccag atttatcagc ataaaccag ccagccggaa 12300 gggccgagcg cagaagtggt cctgcaactt tatccgccctc catccagtct atttattgtt 12360 gccgggaagc tagagtagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 12420 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 12480 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 12540 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 12600 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 12660 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 12720 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 12780 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 12840 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 12900 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 12960 tactcatact cttcctttt caatattatt gaagcattta tcagggttat tgtctcatga 13020 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 13080 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 13140 ataggcgtat cacgaggccc ttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 13200 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 13260 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 13320 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 13380 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 13440 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 13500 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 13560 gtgcaagctt gcatgcctgc agtgcagcgt gacccggtg tgcccctc tagagataat 13620 gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg 13680 aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa tatataatc 13740 tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg 13800 tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc 13860 atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt 13920 agtacatcca tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc 13980 tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta 14040 tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc 14100 tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc 14160 tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg 14220 ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt 14280 tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc 14340 agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg 14400 ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac 14460 cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag 14520 atctccccca aatccacccg tcggcgtacg ccgctcgtcc tccccccccc cccctctcta 14580 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat 14640 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg 14700 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct 14760 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc 14820 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg 14880 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt 14940 tctagatcgg agtagaattc cccgatctag taacatagat gacaccgcgc gcgataattt 15000 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac 15060 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg 15120 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa 15180 tcttaagaaa ctttattgcc aaatgtttga aacgatcgggg aaattcgagc tcttagaact cccaccactt ttggaactcc cccactttt gaaggtctgg cagttgttct tcaatctcca acaaactgtt tccttcttca aggtacgca aaccgtcgca aaacggaaca ttcatttctt gaccaacat aacgtccgga gagttcgtaa accgatcatc cgattcttca ccgctgccac tactactagt tttctcttga tgcttttcgg ctacattct cagcacctcc aattgattga gtagggcctg attctctttc tttagagact cggatttaga cgcaagcgtc tcgtagttat 15540 gctttagcgc gttatactct tgctcaatct gcctcgactt tgatcgcgcg cgtttgttct 15660. ggaccatat cgccacttga cgaggatgaa gcccgagttt atgtgccaac tggtgtttca tccttaactc gggtctcgac tgtgtctcaa acatgtactc taggaaactt atttgtttgt cggtaaatcg tctccgcccc tcgtttcggt tcttcctggt ggttgttact tgttgaagag 15780. acatggatcc gagtcgacct gcagaagtaa caccaaacaa cagggtgaca ctcgacaaaa gaaaaaaaat ccacatatag 15900 ctgctgcata tgccatcatc caagtatatc aagatcaaa tattattaaa acatacttgt 15960 ttattataat agataggtac tcaggttag agcatatgaa tagatgctgc atatgccatc 16020 atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat cgatatttcc 16080 atccatctta aactcgtaac tatgaacgct gtcttgacac accatacag ttccaaatt 16140 aaaataca ccaggtagtt tgaacagta ttctactccg atctagacg atgaacgac 16200 cgcccaacca caccacatca tcacaaccaa gcgaacaaa agcatctctg tatatgcatc 16260 agtaaaaccc gcatcacat gtatacctat cctagatcga tattccatc catcatctc 16320 aattcgtaac tatgaatatg tatggcacac acatacagat ccaaattaa taaatccacc 16380 aggtagtttg aaacagaatt cgtaatcatg gtcatagctg ttcctgtgt gaaattgtta 16440 tccgctcaca attccaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 16500 ctaatgagtg agctactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 16560 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 16620 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 16680 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 16740 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 16800 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 16860 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 16920 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 16980 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 17040 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 17100 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 17160 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 17220 gaagtggtgg cctaactacg gctacactag area tttggtatct gcgctctgct 17280 gaagccagtt accttgggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 17340 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa areatctca 17400 agaagatcct ttgatcttt ctacggggtc tgacgctcag tggaacgaaa acttttctac 17460 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 17520 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 17580 tatatatgag taaacttggt ctgacagttt gaggcaccta tctcagcgat ctgtctattt 17640 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 17700 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 17760 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 17820 gcctccatcc agtctattaa ttgttgccgg gaagctagag tagtagttc gccagttaat 17880 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 17940 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 18060. tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 18360. actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa tgtatttaga aaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 18660 cccttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 18720 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 18780 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 18840 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 18900 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 18960 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 19020 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc 19080 ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgcta 19140 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 19200 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 19260 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 19320 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctcctttt 19380 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 19440 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 19500 agcccttaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 19560 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 19620 aactaaggaa acattttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 19680 cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 19740 cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 19800 acttgctccc tttatttat tctattttat atctaaatta ttaaataaaa aaactaaaaat 19860 agagttttag ttttcttaat ttagaggcta gtcccgaaca gtgtcctgga ggccgcacac 19920 gcttcggccc cgaccctcga ttcggagccc gctccgttcg ttgaggaaga acgattggac 19980 gtcacctcag aggctgcgac attaaaggcg gtcgagtcga acgccgaccc cgcactccgc 20040 gctgcccatt actccgagga gccggattct tctccggcct ccgggccttc catgcccctg 20100 ccgaccgaat ccgattgggc acctgtaatg gagttcaccg ccgcggacat ctttcagtgt 20160 tcacctttca gtgacatcct gaattatctc aagtctctct ctttatcagg agagcccagg 20220 ccggattatg gtctgcgagg ttgggattcg gacgatgaag aaattcaaag cccacccacc 20280 acccacttaa tagccactgt cgacgattta accgacatgc tcgacttcgg ctccgaagac 20340 ttcgacggta tggacgacga aggaggagac gaaccggaac cagcacccac agggcactgg 20400 atatccacca cacacaat 20418 <210> 7 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> 5' Flanking Region (Long Insert) <400> 7 taatggtgag tgtatgcgct tgcgtctgta ttgtgtttaa aaaactcatt ctaagaaact 60 cgtcatacag tgtccaccgt caaggagcca tctagtaggg tgtggatggg catgtgacag 120 gaaccttgtc gtgtgcacgt tgtatgtcgg tcttgttcaa tatgtcgtct gtttcctcgg 180 cttacatgct cttttacgtg tcgtgcaccc aactgatctt cagcatcttt tactttcacc 240 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 300 <210> 8 <211> 385 <212> DNA <213> Artificial sequence <220> <223> 3' Flanking region (long insert) <400> 8 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 60 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc gtcgactcta gaggtccctc 120 gaccatggct gtaaggtcgc cgaatacaag ggcatgggtg gcgaggccta agaggagtat 180 aatttcttag acatttgtca attataaact tcttggtaaa agagcatgta agccgaggaa 240 acagacgaca tattgaacaa gaccgacata caacgtgcac acgacaaggt tcctgtcaca 300 tgcccatcca caccctacta gatggctcct tgacggtgga cactgtatga cgagtttctt 360 agaatgagtt ttttaaacac aatac 385 <210> 9 <21~1> 392 <212> DNA <213> Artificial sequence <220> <223> 5' flanking region (short insert) <400> 9 gagcgcgctt tcctatttag gatccaaatt ggcactgata ctaaactgct tagacgaatc 60 gcctggagta aaatcaacaa gctcggtttc agcagccgac ttaaacttca tcgccgggtc 120 atgctcggtg gttggcttct ttagagacgt catatccgcc aggtcaacat tattttgata 180 aaaattcatc tcctctgccg cacaaacaga ctcagcataa gccgcatcgc cttcttcaca 240 ttctagggct acctttcgac tcccattgag ggagtcccgg attagggggt gtttggaaca 300 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 360 ctctagcttc ccggtaacaa ttaatagact gg 392 <210> 10 <211> 628 <212> DNA <213> Artificial sequence <220> <223> 3' flanking region (short insert) <400> 10 ccaccgttgg acttgctccc tttattttat tctattttat atctaaatta ttaaataaaa 60 aaactaaaat agagttttag ttttcttaat ttagaggcta gtcccgaaca gtgtcctgga 120 ggccgcacac gcttcggccc cgaccctcga ttcggagccc gctgcgttcg ttgaggaaga 180 acgattggac gtcacctcag aggctgcgac attaaaggcg gtcgagtcga acgccgaccc 240 cgcactccgc gctgcccatt actccgagga gccggattct tctccggcct ccgggccttc 300 catgcccctg ccgaccgaat ccgattgggc acctgtaatg gagttcaccg ccgcggacat 360 ctttcagtgt tcacctttca gtgacatcct gaattatctc aagtctctct ctttatcagg 420 agagcccagg ccggattatg gtctgcgagg ttgggattcg gacgatgaag aaattcaaag 480 cccacccacc acccacttaa tagccactgt cgacgattta accgacatgc tcgacttcgg 540 ctccgaagac ttcgacggta tggacgacga aggaggagac gaaccggaac cagcacccac 600 agggcactgg atatccacca cacacaat 628 <210> 11 <211> 23 <212> DNA <213> Artificial sequence <220> <223> HB4 forward primer <400> 11 gggcttcatc ctggtcaagt ggc 23 <210> 12 <211> 30 <212> DNA <213> Artificial sequence<​​<223> HB4 reverse primer <400> 12 tcttgatgct tttctgctac atttctcagc 30 <210> 13 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Bar forward primer <400> 13 cacgcaacgc ctacgactgg acggcc 26 <210> 14 <211> twenty four <212> DNA <213> Artificial sequence <220> <223> Bar reverse primer <400> 14 gtaccggcag gctgaagtcc agct 24 BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 Schematic diagram of the plasmids pIND4-HB4 and pIND-Bar used to obtain IND-ØØ412-7. The schematic shows the CDS of HaHB4 (left) and bar (right), along with their regulatory elements and plasmid backbones. prUbi-1: Promoter region of the maize Ubi-1 gene. prUbi-1 exon: First exon of the maize Ubi-1 gene. prUbi-1 intron: First intron of the maize Ubi-1 gene. HaHB4: CDS of HaHB4. Bar: CDS of bar. Tnos: Terminator sequence of the nos gene. pBR322 origin: ColE1 origin of replication. bla: Bla gene.

[0043] Figure 2 : Plasmid sequence and sequence of interest.

[0044] Figure 3 : The obtained junction sequence (JP) supported by Illumina and PacBio readings and by the PCR amplification product of Sanger sequencing. From the JP, the wheat sequence obtained completely is shown, while only the first 100 bp are shown for the plasmid. The numbers represent the absolute position on the inserted sequence.

[0045] Figure 4: Yield of transgenic lines (events) and parental controls (wild type) from six field trials conducted from 2009 to 2011. Bars represent standard error of the mean (SEM).

[0046] Figure 5 :use Hind III. Bam HI and Ase Southern blot of IND-ØØ412-7 plant DNA digested with I. The blot was hybridized with a DIG-labeled probe for HaHB4 Detection. DNA bands hybridizing to the probe in the IND-ØØ412-7 digest are indicated by white arrows. HB4 soybean transgenic plants (IND-ØØ41Ø-5) were used as positives, and hybridizing bands are indicated by red arrows. The sizes of the DIG-labeled marker VII and III ladder bands are indicated in kb on the left and right sides of the blot, respectively.

[0047] Figure 6 :use Hind III. Bam HI and Ase Southern blot of IND-ØØ412-7 plant DNA digested with I. The blot was hybridized with a DIG-labeled probe for bar Detection. DNA bands hybridizing to the probe in the IND-ØØ412-7 digest are indicated by white arrows. HB4 soybean transgenic plants (IND-ØØ41Ø-5) were used as positives, and hybridizing bands are indicated by red arrows. The sizes of the DIG-labeled marker VII and III ladder bands are indicated in kb on the left and right sides of the blot, respectively.

[0048] Figure 7 : Evaluation of the integrity and size distribution of DNA samples generated by chromosome 2D sorting. Average fragment size: 5951 bp.

[0049] Figure 8 : PacBio read length distribution. The histogram shows the absolute frequency (number of reads) in intervals of 1000 bp read length.

[0050] Figure 9 : Quality scores of PacBio reads. The upper panel shows the quality scores of the base calls as a function of the read position (in percent), and the lower panel as a function of the read position (in bp).

[0051] Figure 10 :contain HaHB4 (red) and bar(Light blue) DNA sequence diagram of the complete (c) and incomplete (i) coding regions of the long insert of IND-ØØ412-7. For better understanding, the insert is subdivided into four segments. a) HaHB4 Incomplete (i) coding region located in Ubi-1 Between the two incomplete fragments of the intron. In addition, there are two bar Coding regions, complete (c) and incomplete (i). Bar (i) is between two Tnos regions. Bar (c) is correctly positioned between its regulatory elements. b) HaHB4 (c) The coding region is located in ubi-1 The adjacent region of the intron and the 5' promoter region are located downstream and upstream of its Tnos region. Bar (i) is located between the two intronic ubi-1 regions. c) Bar (c) is located between the inverted Ubi-1 promoter region and the Tnos region. Bar (i) is located between the partial ubi-1 promoter region and the bla gene. d) The two bar (c) coding regions are located between their complete regulatory elements.

[0052] Figure 11 :contain HaHB4 and bar Figure 1. Schematic diagram of the DNA sequence of the short insert of IND-ØØ412-7, complete (c). The insert is subdivided into three segments for better understanding. b) Bar (c) is located between the inversion and the short sequence prGBL-1 region and the Tnos region. c) HaHB4 The complete (c) coding region is located in Ubi-1 Between the incomplete fragment of the intron and Tnos.

[0053] Figure 12 :For wheat( T. aestivum ) was used to perform blasting on the coverage in JP (available at http: / / plants.ensembl.org / ).

[0054] Sequence Description

[0055] SEQ ID No. 1: Long insert without flanking regions

[0056] SEQ ID No. 2: Short insert without flanking regions

[0057] SEQ ID No. 3: pIND4+HB4

[0058] SEQ ID No. 4: pIND4+BAR

[0059] SEQ ID No. 5: Long insert with flanking regions

[0060] SEQ ID No. 6: Short insert with flanking regions

[0061] SEQ ID No. 7: 5' flanking region (long insert)

[0062] SEQ ID No. 8: 3' flanking region (long insert)

[0063] SEQ ID No. 9: 5' flanking region (short insert)

[0064] SEQ ID No. 10: 3' flanking region (short insert)

[0065] SEQ ID No. 11: HB4 forward primer

[0066] SEQ ID No. 12: HB4 reverse primer

[0067] SEQ ID No. 13: Bar forward primer

[0068] SEQ ID No. 14: Bar reverse primer

[0069] Detailed description

[0070] The following definitions and methods are provided to better define the present invention and guide those trained in the technology to implement it. Unless otherwise specified, the terms used herein should be interpreted as having the same meanings that are commonly given to them by those trained in the relevant technology. Example

[0071] Example 1: Plasmid pIND4-HB4 and pIND4-Bar Construction

[0072] The vector used to generate wheat IND-ØØ412-7 is based on a series of plasmids that use the maize ubiquitin promoter to direct gene expression in plants (Christensen and Quail, 1996). This promoter has been shown to be very efficient in monocots (Christensen et al., 1992). These plasmids use the vector base pUC8 Cloning was performed, and since the clone 1992pb Ubi-1 maize ubiquitin gene Pst1 This fragment is a functional promoter and consists of the following: 899pb gene Ubi-1 sequence, 83 pb of 5'-untranslated region sequence from the first exon and 1010 pb of the first intron sequence to reconstruct Pst Ends at 1 site. Exists in genes Ubi-1The original intron of the 5'-untranslated region was retained in all these vectors because previous studies have shown that introns stimulate transgene expression in cereal plants (Vasil et al., 1993).

[0073] Based on the two plasmids in this series, pIND4-HB4 and pIND4-Bar , has been used for the genetic modification present in IND-ØØ412-7.

[0074] pIND4-HB4 (SEQ ID NO: 3)

[0075] This plasmid has a multiple cloning site, where BamHI For gene cloning HaHB4 The restriction site is located after the sequence of the maize Ubi-1 gene promoter, the first exon and the first intron of the gene. prUbi-1 / Ubi1-Exon / Ubi-1Intron / HaHB4 Designed to obtain constitutive expression of HAHB4. After the multiple cloning site and HaHB4 After the CDS of the vector, the vector has the Agrobacterium tumefaciens ( A. tumefaciens ) of the nopaline synthase gene terminator, including the polyadenylation signal.

[0076] pIND4-Bar (SEQ ID NO: 4)

[0077] This plasmid has also been Bam The HI site will Ubi-1 The regulatory region binds to Streptomyces hygroscopicus ( S. hygroscopicus )of bar The construct is constructed by the coding region of the gene. prUbi-1 / Ubi1-Exon / Ubi-1Intron / bar This allows the selection of transgenic plants that express PAT and are tolerant to the herbicide glufosinate, which is rendered inactive by the enzyme (Thompson et al., 1987). bar Following the coding region, the vector also contains the Agrobacterium tumefaciens ( A. tumefaciens ) of the nopaline synthase gene terminator, which includes a polyadenylation signal.

[0078] Example 2: Transformation of wheat plants and selection of event IND-ØØ412-7

[0079] The co-transformation method used to obtain event IND-ØØ412-7 is based on the methods developed by Barcelo and Lazzeri (1995), Pastori et al. (2001), and Rasco-Gaunt et al. (2001). These methods are also used in several research centers.

[0080] First, wheat grains are harvested and sterilized. Grains are collected from the ears and sterilized. Then, the embryos are isolated and their rachises are removed to prevent premature germination. The scutellum is placed in a suitable medium in the center of a culture dish, with the undamaged area of ​​the explant facing upward to expose it to microparticle impact.

[0081] For bombardment, the gene gun PDS-1000 / He (Biolistic® PDS-1000 / He Particle Delivery System, BIO-RAD) was used to bombard the cells with the plasmids from the two cells of interest. pIND4-HB4 and pIND4-Bar The DNA-coated gold particles are propelled into the interior of the explant's cells.

[0082] After the bombardment, the treated explants are placed in fresh culture medium under the necessary hormone conditions for producing embryonic callus. Once the embryo has developed, the selection process begins after three weeks. The selection agent used in this case is glufosinate-ammonium.

[0083] Seedlings that survived the selection rounds and developed a good root system due to PAT expression were transferred and placed in a growth chamber. At this time, samples were obtained and confirmed using PCR. bar and HaHb4 Once the plants develop, they are transferred to larger pots, where they grow until harvest.

[0084] Event Selection

[0085] Transgenic plants were generated following a bioelastic protocol using the British variety Cadenza. The first multiplication (T1 seeds) were transferred to a growth chamber between August and December 2007. Approximately 20 individuals from each transgenic event were sampled and assayed for segregation by PCR (Table 1).

[0086] Table 1. Segregation test. Lines with p-values ​​> 0.15 were transferred to the next generation.

[0087]

[0088] Selfed lines (segregating 3:1 in T1) derived from selected events were sown under hail shelter in August 2008. The growth and development of these lines were characterized throughout the growing season (Table 2).

[0089] Table 2. Phenology.

[0090]

[0091] Table 2. Phenology (continued).

[0092]

[0093] During the vegetative stage, plants were sampled for PCR analysis to identify homozygous lines. Six field trials were conducted between 2009 and 2011 in Monte Buey (Córdoba, 2009), Gutenberg (Córdoba, 2010), Camet (Buenos Aires, 2011), Corral de Bustos (Córdoba, 2011), Daireaux (Buenos Aires, 2011), and Villa Saboya (Buenos Aires, 2011) using three selected homozygous lines.

[0094] The experiment included three homozygous lines (events) and a wild-type (parental control). Yield data were analyzed using Infostat (Di Rienzo et al., 2010) using a 95% confidence level, randomized complete block design ANOVA. Data from each site were pooled, and sources of variation included genotype, environment, and genotype-environment interactions. Means were compared a posteriori using Fisher's least significant test (α = 0.05).

[0095] As for the effect of genotype, the variance analysis showed statistically significant differences (p = 0.0003, Figure 4 In addition, a statistically significant genotype-environment interaction was detected (p < 0.0001). This interaction was achieved by exchanging line Ta.IV.ii.a.4 with the control yield at one site ( Figure 4 ) to explain this. In the combined analysis, all transgenic lines provided yields higher than the parental control. Among the lines, Ta.IV.ii.a.12 provided the highest mean yield across all sites. This transgenic line was selected for further characterization and renamed IND-ØØ412-7.

[0096] Example 3: Characterization of DNA sequences in wheat event IND-ØØ 412-7

[0097] Due to the complex nature of the wheat genome, a strategy was designed to reduce the background genomic information in the wheat genome to interpret the insert structure, sequence and its flanking sequences. Using the DArT technology platform (Diversity Arrays Technology, Jaccoudy et al., 2001), the vector chromosome was identified. Once the HaHB4The vector chromosome was isolated using chromosome sorting technology (Doležel et al., 1989; Jan Vra'na et al., 2000; Suchánková et al., 2006; Šimková et al., 2008). The sequencing strategy to identify the insertion region of IND-ØØ412-7 in the isolated chromosome consisted of a combined next-generation sequencing approach using two complementary sequencing technologies: Illumina and Pacific Biosciencies. Illumina generates high-throughput, short, 100-bp reads from both sides of a 500-bp fragment sequencing library. PacBio, on the other hand, generates low-throughput, single-molecule reads from a 15-20 kb fragment library.

[0098] As insert size increases, the likelihood that the polymerase will pass through the sequence more than once decreases. For this reason, larger sequences have a higher error rate than smaller sequences. To address this issue, a specific algorithm has been developed that uses smaller sequences obtained via Illumina to correct these sequences. Using this algorithm, PacBio RSII sequences were corrected, and accurate and reliable sample assemblies were obtained (Koren et al., 2012).

[0099] a) Southern blot analysis.

[0100] The copy number of the gene of interest was determined by Southern blot analysis in homozygous IND-ØØ412-7 plants.

[0101] Leaf tissue from homozygous IND-ØØ412-7 plants or Cadenza plants grown in the greenhouse or in the field was used for DNA isolation. One gram of leaf tissue from IND-ØØ412-7 or Cadenza was flash-frozen using liquid nitrogen and ground into a fine powder using a pre-chilled pestle and mortar. DNA was extracted using the Qiagen DNeasy Maxi Prep Kit according to the manufacturer's protocol. After elution, DNA was precipitated by adding 1 / 10 volume of 3M sodium acetate and 2-3 volumes of 100% ethanol. The precipitate was washed with 70% ethanol and suspended in 80 µL of 1x TE buffer. DNA was quantified using a QuBit fluorometer. The DNA concentration for IND-ØØ412-7 was 1100 ng / µL, and for Cadenza it was 900 ng / µL.

[0102] Genomic DNA from event IND-ØØ412-7 was digested with three enzymes: HindIII 、 BamH1 and AseIFor each 50 µL digestion reaction, mix 5 µg of genomic DNA with HindIII, BamHI, or AseI enzyme at a concentration of 10 U / µg DNA. Digest the samples overnight (~16 hours) at 37°C. For control plasmid digests, use 100-200 pg of plasmid DNA.

[0103] Restriction digests of IND-ØØ412-7 and Cadenza genomic DNA were loaded onto a 0.7% agarose gel along with DIG-labeled molecular weight marker VII (Roche catalog number 1669940910). The samples were run overnight at 50 V. The gel was incubated twice in denaturation buffer for 30 minutes each. The denatured gel was washed in transfer buffer for 15 minutes and then subjected to alkaline transfer (described below).

[0104] The pIND-4 plasmid has a HindIII Site, very close to 5´ prUBI-1 There are no other HindIII Site presence ( Figure 1 If there is a single complete insert containing the sequence of interest in the IND-ØØ412-7 genome, HaHB4 The minimum fragment size for hybridization of the probe will be 2.842 kbp, and the minimum fragment size for hybridization of the bar probe will be 2.860 kbp.

[0105] In addition, the pIND-4 plasmid has a BamHI Location, very close HaHB4 and bar The start codon of CDS. There is no other BamHI Site presence ( Figure 1 ). Again, if there is a single complete insert containing the sequence of interest in the IND-ØØ412-7 genome, HaHB4 The minimum fragment size for hybridization of the probe will be 825 bp, and the minimum fragment size for hybridization of the bar probe will be 843 bp.

[0106] Finally, there are five AseI Restriction site ( Figure 1 ), two in UBI-1 within the sequence of interest in an intron, and three in the vector, two of which are close to Tnos at the end and another at bla Inside the gene. Completely within the insert AseI The digestion should release a 1.313 kbp long DNA segment containing HaHB4 The probe binds to the target. barThe probe will detect a DNA fragment of 1.331 kbp. This consideration assumes a single intact insert.

[0107] Following the procedure outlined in the Roche PCR DIG Probe Synthesis Kit (Cat. No. 11636090910) and using the oligonucleotides mentioned in Table 3, the DIG probes for HaHB4 and bar Molecular probes for CDS.

[0108] Table 3. List of primers used to prepare probes used in Southern blot analysis.

[0109]

[0110] DNA was transferred from an agarose gel using a Turboblotter-Rapid Down Transfer System (Whatman). DNA was transferred to a 12 x 21 cm nylon membrane (Nytran™ SuPer Charge, Sigma-Aldrich Co., St. Louis, MO) for 4 hours. The membrane was washed in neutralization buffer (0.2 M sodium phosphate, pH 6.8). DNA was permanently crosslinked to the membrane using an Ultraviolet Crosslinker (CL-1000) with two 1500 mJ exposures.

[0111] The membrane was incubated in 50 ml of Roche DIG EasyHyb hybridization buffer (Cat. No. 11 603 558 001) at the pre-calculated hybridization temperature (45°C and 55°C for bar gene and HaHB4 gene probes, respectively) on an orbital shaker.

[0112] Dilute 35 µl and 45 µl aliquots of bar and HaHB4 probes by adding 65 and 55 µl of 1x TE buffer, respectively. Incubate the probe solutions at 95°C for 10 minutes and cool to 4°C for 2 minutes. Add these to 8.75 ml of DIG hybridization buffer and pour into the bottom of the hybridization bottle. Incubate the membrane in a molecular hybridizer (VWR Scientific Products) equipped with an orbital shaker at the specified hybridization temperature for 16 hours.

[0113] After hybridization, the membrane was washed with wash buffer according to the instructions provided with the Roche DIG Luminescent Detection Kit. After blocking with 1x blocking reagent for 1 hour, the membrane was incubated in a solution containing 50 ml of 1x blocking reagent and 5 µl of anti-digoxigenin-AP for 30 minutes. The membrane was washed twice with wash buffer for 30 minutes each, and finally treated with detection buffer for 5 minutes. At this point, the membrane was placed in a KPL hybridization bag (KPL catalog number 60-00-51). 5 ml of CSPD solution from the DIG Luminescent Detection Kit was evenly applied to the membrane. The membrane was incubated with the CSPD solution at room temperature for 5 minutes. The hybridization bag with the membrane was heat-sealed and incubated at 37°C for 15 minutes. The hybridization bag was placed in a cassette containing Kodak BiomaxLight Film (catalog number 178 8207) in a dark room and exposed for 20 minutes. The film was developed in the dark using a Konika QX-60A X-ray film processor. Subsequent exposures were performed after 1 or 2 hours, as needed.

[0114] use HaHB4 Analysis of the hybridization bands obtained with the probe allowed to hypothesize the presence of three copies of this CDS ( Figure 5 ).

[0115] On the other hand, using bar Analysis of the hybridization bands obtained with the probe allows us to hypothesize that BamHI The presence of seven copies of this CDS ( Figure 6 ). However, when considering HindIII and AseI The lower number indicates possible internal DNA rearrangement ( Figure 6 ).

[0116] In short, Southern blot results indicated a complex insertion pattern with possible internal DNA rearrangements, suggesting that genomic sequencing approaches should be necessary to resolve the insertion structure in the IND-ØØ412-7 event.

[0117] b) Identification and isolation of the chromosome containing the insertion sequence in IND-ØØ412-7

[0118] DArT was used to identify the mitochondrial locus located on chromosome 2D of the wheat genome. HaHB4 Each polymorphic DNA fragment (which corresponds to a spot on the plate) is actually a molecular marker that can be used for genotyping of paired cross isolates (presence or absence of the marker in the isolate material) and detecting the association of these markers with agronomically interesting traits (the region of polymorphism). In the case of the IND-ØØ412-7 event, this technology was used to genotype the F2 generation of the cross IND-ØØ412-7 x Bag17 and search for associations of these markers with the agronomically interesting traits. HaHB4 The possible association of the transgene was determined in each F2 individual HaHB4 The presence or absence of and, after genotyping, determination of DArT markers and HaHB4 The transgene showed an association with a marker located on chromosome 2D (http: / / www.cerealab.unimore.it / ). Only one marker showed an association with HaHB4 The fact of association may be due to the fact that there is no saturated marker map available for this particular chromosome (Jia et al., 2013; http: / / ccg.murdoch.edu.au / cmap / ccg-live / cgi-bin / cmap / viewer).

[0119] HaHB4 The CDS is most likely located in the long arm near the centromere associated with marker Gpw3105.

[0120] Using this information, 5 ug of chromosomal 2D DNA was obtained, which was suitable for Illumina library preparation. However, due to the nature of the post-sort DNA amplification process, the DNA was partially suitable for PacBio libraries ( Figure 7 ).

[0121] Diversity panels were generated using DNA from IND-ØØ412-7 and wild-type Cadenza.

[0122] Genomic DNA was extracted from seedlings. Approximately 5 ng of DNA from each genotype was purified by lysing with 2 U of restriction enzyme ( EcoRI 、 PsI and MspI ) digestion for 1 hour. After digestion, 2 μL of ligation mix was added and the sample was incubated at 37°C for 3 hours. The ligation mix contained 0.2 μL of T4 ligase (New England Biolabs), 0.2 μL of 50 mM ATP, 1.2 μL of MilliQ water (MQ), and 0.1 μL of 50 pmol / μL ( MspI ) and 5 pmol / µL ( EcoRI and PsI ) with the appropriate specific enzyme. After ligation, the mixture was diluted to a final volume of 500 µL with MQ water, and 2 µL was used as template for the PCR reaction. Reactions were performed in a final volume of 50 µL with 2 U of Red Taq polymerase (Sigma) and primers. After an initial incubation at 95°C for 3 minutes, the reaction followed the following protocol: 30 cycles of 94°C for 30 seconds, 60°C for 45 seconds, and 72°C for 1 minute. A final extension was performed at 72°C for 8 minutes.

[0123] The resulting amplicon was ligated into the PCR2.1TOPO vector (Invitrogen) and subsequently transformed with heat-shocked Escherichia coli TOP10F' (Invitrogen) cells according to the manufacturer's instructions. Transformants were selected on medium containing ampicillin and X-gal. Single white colonies (containing the recombinant plasmid) were transferred to a test tube containing 20 μL of 10% glycerol using an inoculating loop. A 5 μL aliquot was obtained from each sample in glycerol and transferred to a test tube containing 45 μL of PCRmaster mix RedTaq tube (Sigma) and 15 pmol of each M13 forward and M13 reverse primer. The analytical cycler used was 95°C for 5 minutes for 1 cycle; 94°C for 30 seconds, 52°C for 30 seconds, and 72°C for 1 minute for 35 cycles. After amplification, the PCR product was precipitated with 1 volume of isopropanol at room temperature and washed with 100 μL of 70% ethanol. The ethanol was removed and the product dried at room temperature.

[0124] The product was resuspended in MQ water, 3x SSC, or 1x SSC + 0.01% sarkosyl (Sigma) to a final concentration of ~20 ng / µL. The purified fragment was transferred to a 384-well plate (Genetix) and plated in six replicates per channel on a Polysine™ chip (Menzel Glazer) using the Affymetrix™ 417 System. After one day of fixation, the chip was processed according to the protocol published at http: / / microarrays.org / protocols.html (2001), with the slight exception of using sodium borohydride (Sigma) as the blocking solution instead of succinic anhydride and 1-methyl-2-pyrrolidone in PBS buffer, pH 7.4 (137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO, 2 mM KHHPO). The chip was immersed in boiling water for 30 seconds to denature the DNA, followed by immediate immersion in ice-cold 100% ethanol for 10 seconds. The chip was dried at room temperature.

[0125] After PCR amplification, the product was purified using a column (Qiagen) or precipitated by adding isopropanol to remove excess dNTPs. Cy3 and Cy5 (Pharmacia) fluorescent dyes were incorporated using the Deca-random-prime DNA labeling kit (Fermentas) according to the manufacturer's instructions.

[0126] Samples labeled with Cy3 and Cy5 dyes (5 µL each) were mixed with 2 µL of 20 μg / µL herring sperm DNA (Sigma) dissolved in Express Hybridization Solution (Clontech) and denatured at 96°C for 3 minutes. The denatured probes were mixed with 10-15 µL of hybridization solution, transferred directly to the microarray surface, and covered with glass (24 x 24 mm, Mediglass). The array was immediately placed in a humidified chamber and hybridized overnight at 65°C. After hybridization, the array was washed with 1x SSC containing 0.1% SDS for 5 minutes, 1x SSC for 2 minutes, 0.2x SSC for 2 minutes, and 0.02x SSC for 20 seconds.

[0127] The chips were scanned using an Affymetrix 418™ scanner. The intensity of each signal was analyzed using Scanalyse v.2.44 (Standford University) and GenePix Pro v.3 (Axon instruments) programs.

[0128] c) Junction sequence analysis and insert structure in IND-ØØ412-7 using Illumina and PacBio sequencing

[0129] Southern blot analysis results suggest a complex pattern of transgenic insertion. Therefore, the isolated chromosome 2D DNA library was sequenced using the Illumina Hiseq platform. We generated more than 1.2 billion reads in a 2x100bp paired-end configuration, representing more than 124 Gb of sequencing data. Based on non-redundant mappable reads on single-copy genes such as PpoD1b located within chromosome 2D, the sequencing coverage of the entire chromosome was estimated to be between 40 and 50X (He et al., 2009). The sample enrichment of chromosome 2D was verified by mapping the reads to known single-copy genes such as LOXs located outside the chromosome (Feng et al., 2012). In these cases, the coverage was estimated to be between 1 and 5X, thereby confirming the highly enriched DNA in the chromosome 2D sequence.

[0130] Reads mapped on the plasmid used for transformation allowed us to validate the Southern blot results. We concluded that we had obtained 3 complete or near complete copies of HaHB4 CDS, and 8 complete or near-complete copies based on 400X coverage bar CDS.

[0131] Several other plasmid elements were verified with coverage up to 1200X.

[0132] Using the junction sequence analysis (JSA) protocol (Kovalic et al., 2012) of Illumina reads, up to four different flanking sequences were identified, with chimeric junction sequences containing wheat DNA and several plasmid elements. In short, the Illumina data also indicated a complex pattern of insertion events in IND-ØØ412-7, including internal plasmid rearrangements.

[0133] Therefore, PacBio long sequencing reads were generated to assist in the scaffolding process of Illumina read assembly. PacBio long reads will allow reading through complex internal structures and rearrangements.

[0134] Since the DNA sample quality obtained after chromosome sorting allowed us to generate libraries with an average size of approximately 5 Kb, PacBio's P4-C2 chemistry was used. This chemistry produces reads with an average length of 5.5 Kb. 20 SMART pools were used to generate 1,806,197 reads for a total of 3.5 Gb of sequencing data, with an average read length of approximately 2 Kb and a maximum of 13 Kb. Based on a chromosome 2D size of 800 Mb, the coverage of the entire chromosome was estimated to be 4.7X. PacBio statistics are shown in Table 4, and the read length distribution and Q score are shown in Figure 8 and Figure 9 middle.

[0135] Table 4. PacBio statistics.

[0136]

[0137] Based on a combined analysis of sequencing data from Illumina and PacBio, the flanking sequences of the insertion were first defined.

[0138] A four-parameter set was established as a cutoff to determine the true identity of the flanking sequences:

[0139] 1. Supported by Illumina data,

[0140] 2. Supported by PacBio data,

[0141] 3. Supported by Illumina coverage as a single copy, and

[0142] 4. Supported by PCR amplification from IND-ØØ412-7 DNA.

[0143] Four different flanking sequences passed all filters meeting the selected criteria, designated JPLa, JPLb, JPSa, and JPSb.

[0144] A similar approach was used to determine all internal chimeric plasmid-plasmid junction sequences, but taking into account the Illumina coverage of the individual plasmid elements included. Internal junction sequences that passed all filters were expanded using Illumina data and mapped on long PacBio reads to generate the final assembly. The quality of the final assembly was compared a posteriori to the initial Southern blot data, and the results were validated.

[0145] Briefly, two different insertions of a plasmid into a highly repetitive region of chromosome 2D of the wheat genome were described. One insertion was 47,611 bp long and the other was 20,418 bp long, for a total of 68,029 bp insertions in the IND-ØØ412-7 event ( Figure 10 and 11 ). The insertion results in three copies of HaHb4 CDS, seven copies of bar CDS, 16 copies of bla gene and four copies of gus CDS. In longer insertions ( Figure 10 ), it is possible to identify the complete downstream of possible functional regulatory elements HaHB4 Coding region (red) (sub-image b). On the other hand, the complete bar The coding region (light blue) is observed in panels a, b, c, and d. In panels (a) and (d) it is possible to identify bar The regulatory elements are complete and in the correct position. Bar CDS potentially encodes a functional protein. Therefore, only one HaHB4 A copy and a bar copy, without complete regulatory elements.

[0146] d) Detection, structure, and copy number of vector sequences unrelated to the gene of interest

[0147] Evident from analysis of the insertion events was the presence of plasmid vector sequences and other coding regions unrelated to the gene of interest and regulatory regions introduced during wheat transformation.

[0148] Elements of the plasmid backbone detected in the insertion of IND-ØØ412-7 confer ampicillin resistance in bacteria bla gene, and the bacterial origin of replication of the pBR322 plasmid ( Figure 10 and 11 ).about bla It is important to mention that it has no regulatory elements for expression in wheat, and the bla None of the copies are localized near eukaryotic regulatory elements.

[0149] All plasmid backbone sequences in the IND-ØØ412-7 insert were of proven origin in the vectors pIND4-HB4 and pIND4-Bar used for transformation, as confirmed by nucleotide sequence identity using blast.

[0150] However, the corresponding gus The sequence of the gene (Jefferson et al., 1987) and the wheat gbl1 The promoter of the gene (Jones, 2005). These sequences were derived from a third plasmid used to monitor transformation efficiency; however, these elements were incorporated during the integration process. gus The CDS is present in four copies, while the gbl1 promoter is present in five copies ( Figure 10 and 11 ). The third plasmid is also used to HaHB4 and bar A series of derivatives adulterated with wheat.

[0151] e) Location of IND-ØØ412-7 insertion in the wheat genome

[0152] The insertions were located in wheat chromosome 2D. These insertions were detected in non-annotated sequences and it is very likely that they interrupt the coding sequence for the reasons described below.

[0153] All flanking wheat sequences corresponded to DNA segments with high similarity to retrotransposons, indicating an insertion event within a highly repetitive DNA region ( Mayer et al., 2014 ).

[0154] To confirm this, JP (junction) was blasted against the IWGSC project database using the EnsemblPlants website (http: / / plants.ensembl.org / Triticum_aestivum / Info / Index). Multiple hits were observed for multiple wheat (T. aestivum) chromosome sites in each case. Although uneven in some cases, full sequence coverage ( Figure 12 Because the IWGSC project is still under development, another blast was performed against all remaining sequenced cereal genomes present in the EnsemblPlants database (Triticum urartu, Aegilops tauschii, and Hordeum vulgare), which yielded the same pattern of multiple hits across multiple chromosomal loci. Sequences that are present in multiple chromosomal loci and conserved across different organisms tend to be repetitive sequences.

[0155] JP sequence is also blasted to NCBI non-redundant database.Two of these sequences (JP is short, JPSa and JPSb) match known retrotransposon elements (Table 5).Although other two JP sequences (JP is long, JPLa and JPLb) do not have significant hits to NCBI database, they are highly conservative in wheat database and are repeated many times in multiple chromosomes, strongly indicating unknown repetitive DNA elements.

[0156] Table 5. Best hit examples of JP sequences blasted against the NCBI non-redundant database

[0157]

[0158] L.: Length

[0159] IND-ØØ412-7 Insertion Stability and Integrity

[0160] The insert detaches with sexual transfer

[0161] Plants homozygous for the IND-ØØ412-7 insertion were crossed with the commercial variety Baguette 17 to produce the F1 generation. After selfing the F1 plants, 349 F2 seeds were obtained. Plants grown from these seeds were used for segregation analysis. DNA was extracted from young leaves. Prior to DNA extraction, leaf tissue was frozen in liquid nitrogen and finely powdered in a mortar and pestle or in a 96-well microprep tube. Depending on the amount of DNA required for the experiment, plant DNA was extracted using the following techniques:

[0162] CTAB method:

[0163] Genomic DNA was extracted using a cetyltrimethylammonium bromide (CTAB)-based method (http: / / irc.igd.cornell.edu / Protocols / DoyleProtocol.pdf). Briefly, 600 μL of CTAB buffer (2% w / v CTAB, 100 mM TrisHCl, 20 mM EDTA, 1.4 M NaCl, and β-mercaptoethanol) and 5 μg of RNase A were added to approximately 100 mg of ground leaf tissue and incubated at 55–60°C for 15–20 minutes with intermittent mixing. 600 μL of chloroform was added to the sample and mixed by hand for 2–3 minutes, followed by centrifugation at 10,000 rpm for 8 minutes. The upper aqueous phase was placed in a clean microtube, and the DNA was precipitated with 400 μL of isopropanol. The sample was centrifuged at 12,500 rpm for 10 minutes to obtain the precipitated DNA. The sample was centrifuged at 12,500 rpm for 5 minutes, and the DNA pellet was washed with 300 μL of 70% ethanol. The DNA pellet was air-dried and then resuspended in 100 μL of TE buffer (10 mM TrisHCl, 1 mM EDTA, pH 8.0). All extracted DNA was stored in a 4°C refrigerator or a -20°C freezer.

[0164] To ensure the integrity of the samples, end-point PCR was performed for wheat endogenous control using specific primers. Then, end-point PCR was performed using different primer combinations to determine HaHB4 and bar Presence of CDS. Chi-square (χ2) analysis was used to assess the expected 3:1 segregation (presence or absence of the CDS of interest).

[0165] Table 6 summarizes the results obtained by PCR analysis.

[0166] Table 6. Results of segregation analysis by PCR.

[0167] total ++ +- -+ -- 349 259 0 0 90

[0168] Total: number of individuals in the experiment;

[0169] HaHB4 + bar +: For HaHB4 and bar the number of CDS-positive individuals;

[0170] bar-HaHB4 +: For HaHB4 the number of CDS-positive individuals;

[0171] HaHB4-bar +: For barthe number of CDS-positive individuals;

[0172] HaHB4-bar -:for HaHB4 and bar Number of individuals who were CDS negative.

[0173] Table 7 shows the statistical analysis.

[0174] Table 7. Statistical analysis of separation results.

[0175]

[0176] The values ​​of χ2 (degree of freedom = 1, α = 0.05) did not show statistically significant differences between the expected and observed results, indicating that for each CDS analyzed ( HaHB4 and bar ), with a Mendelian segregation of 3:1.

[0177] The results obtained in the F2 generation showed that both transgenes were stably inherited during the sexual transfer. According to Mendelian criteria, the two genes had a 3:1 segregation in the segregating population. Finally, even though the two CDSs came from a co-transformation process by bombardment, the two genes were related because each individual F2 analyzed had a 1:1 ratio for both CDSs, i.e. bar and HaHB4 , provides the same results.

[0178] Previous analyses were performed using oligonucleotides that addressed nearly the entire CDS.

[0179] In confirming bar and HaHB4 After confirming the stability of the complete CDS, it was decided to proceed with the characterization of other genetic elements or parts thereof as well as flanking sequences. HaHB4 、 bar and bla For the purpose of transgenic copying, oligonucleotides were designed that hybridized with all copies (complete and incomplete). In addition, four insertion plant junctions were detected to determine their separation in the F2 generation. These studies were carried out in 92 individuals of the F2 group. The results are shown in Table 8.

[0180] Table 8. Statistical analysis of separation results

[0181]

[0182] For the analyzed data, the χ2 values ​​(df=1, α=0,05) in the F2 generation did not show a statistically significant difference between the observed and expected 3:1 segregation ratio.

[0183] For the three components of the IND-ØØ412-7 tested ( HaHB4, bar and bla ) and for the four insertion plant junctions (JPSa, JPSb, JPLa, and JPLb), all transgenic plants analyzed provided consistent results. These results support the conclusion that the IND-ØØ412-7 construct is located at a single locus within the wheat genome and is inherited according to Mendelian inheritance criteria.

[0184] Example 4: Yield increase as an indicator of abiotic stress tolerance

[0185] Fifteen field trials were conducted across multiple experimental sites and planting dates to evaluate the yield and yield components of the transgenic event IND-ØØ412-7 and the parental non-transgenic control line (cultivar Cadenza). The sites were located within Argentina's primary wheat-producing region, between latitudes 32° S and 38° S. This region encompasses the diverse environmental conditions for wheat crop production in Argentina. The trials included representative locations from the pampa wheat region described by Kugler and Godoy (1974): IIN (7 trials), IIS (6 trials), and IV (2 trials). At each site, planting dates were adjusted to farmers' typical practices, starting in May / June at northern sites and in July at southern sites. Planting and harvest dates are reported in Table 9.

[0186]

[0187] Register weather conditions during the growing season for each place.The scope of the annual rainfall registered in the place is from 424 mm to 1497 mm (table 10) for test H13 and G12 respectively, shows that the weather changes widely in place and year.During the growing season (June to December) during 2013, the cumulative rainfall registration value was all lower than 500 mm for all places.The test planted in 2013 had the drought stress condition of higher probability than the test planted in 2012.For 2012, in all places, for the rainfall value of the same time, higher than 500 mm (table 10).

[0188]

[0189] Field trials were conducted at each location using a randomized complete block design. Four replicates were evaluated at each location. The plots consisted of seven rows separated by 0.2 m and 5 m in length. All field trials were adjacent to the plots of seven rows and 5 m in length. Prior to planting, seed stocks of IND-ØØ412-7 and Cadenza were tested by PCR to confirm the presence of the seeds. Hahb4 All seeds were treated with insecticide (thiamethoxam, 0.4 mL kg-1 ) and fungicides (Difenoconazole, 0.2 mL / kg -1 Metalaxyl-M, 0.15 mL kg -1 ) Use the treatment methods described on the corresponding product label.

[0190] All chemicals applied to the crops were in accordance with farmers’ usual practices. Herbicides applied before sowing in the fall consisted of glyphosate (2.0 L ha -1 ) and metsulfuron-methyl (6.8 g ha -1 ). Fungicides were applied to control foliar fungal diseases in trials P12, D12, A12-1, A12-2, F12, H12, G12-1, G12-2, and I12. Fungicides were sprayed once in each trial when 50% of the plots reached growth stage 5.1 (Zadoks, 1974). Insecticides were sprayed at Zadoks growth stage 3.1 in trials A12-1, A12-2, D12, F12, and P12 to control stink bugs (Virus spp. Nezara sp. ), Wall Bug species ( Piezodorus sp. )and Dichelops sp. ). In contrast, trials P13, D13, A13, F13, H13, and I13 grew without fungicide or insecticide spraying due to low disease and pest pressure. The fertilizers and chemicals used in each trial are described in Table 11.

[0191]

[0192]

[0193] Agronomic data of each field trial were evaluated. Agronomic variables consisted of: early standing (NPL), number of ears m -2 (NE), number of grains per ear (NGE), thousand kernel weight (PMG), test weight (PH), yield (REND), biomass aerial (BMA) and harvest index (IC).

[0194] Early establishment is calculated by counting the time before tillering at 0.6 m 2 NE, BMA, grain weight (PG), PMG and IC were evaluated based on 0.6 m 2 PMG is based on 10 g of grain sample harvested from each field. NGE is calculated as follows:

[0195] NGE = (PG / NE) x 1000 / PMG Equation [1]

[0196] pH was evaluated using the standard Argentine protocol for wheat commercialization (buyer's grain scale). Yield was evaluated based on the middle 5 columns of each field for trials A12-1, A12-2, D12, F12, and H12. In the remaining trials, yield was evaluated based on the entire field (7 columns). Yield was corrected for grain moisture (13.5%) and expressed as kg ha -1 .

[0197] Data for transgenic events and the control, Cadenza, were analyzed using Infostat (Di Rienzo et al., 2010) using ANOVA with a 95% confidence level and a randomized complete block design. Comparisons were made between transgenic events and Cadenza in a combined site analysis for REND, PMG, PH, NPL, NE, NGE, BMA, and IC. In this analysis, data from each site were combined, and the sources of variation included genotype (transgenic and control), environment, and genotype-environment interactions.

[0198] In the combined site analysis, three statistically significant differences were detected from eight comparisons between IND-ØØ412-7 and controls (Table 12). Statistically significant differences were detected between IND-ØØ412-7 and conventional controls for REND (p=0.0103), PMG (p=0.0039), and PH (p=0.0430). Furthermore, no statistically significant assay-genotype interactions were detected.

[0199] The REND of IND-ØØ412-7 was 133 kg ha higher than that of the control. -1 , representing an average yield increase of 5%. In contrast, IND-ØØ412-7 showed lower PMG and pH values ​​than the control. No consistent differences in period or biotic stress responses were observed between the event and the parental control. Overall, the significant difference in yield between IND-ØØ412-7 and the control suggests that the transgenic event produced more seeds per unit area of ​​land. Smaller seed size may occur due to a lower source-to-sink ratio during seed filling (which also results in lower pH). The differences between IND-ØØ412-7 and the control were 5% and 2% for PMG and pH, respectively.

[0200]

Claims

1. Be included in the event IND- -7 recombinant DNA molecules, Features Comprising SEQ ID No: 5 and 6, and comprising event IND- Wheat seeds of -7 have been deposited under ATCC accession number PTA-126141.

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