A gel imaging analysis device integrating imaging and analysis

Abstract

The invention relates to the field of detection instruments, and discloses a gel imaging analysis device integrating imaging and analysis, which solves the problem that the operator's hands are easily damaged by long-term exposure to ultraviolet rays during gel cutting, and the structure of nucleic acids in the gel is easily destroyed by long-term ultraviolet rays. The device comprises a body; the invention uses the cooperation between a second crossbar, a slider and a first spring, and when the control of a machine cover is released, the first spring that was originally stretched is reset to make the slider move backward, and the cooperation between the slider and a second rotating plate and the slide plate and the first crossbar makes the slide plate move leftward along the first crossbar, and the cooperation between the first rotating plate and a fixed plate and the machine cover and a connecting shaft makes the machine cover rotate along the connecting shaft, so that the machine cover can rotate and the sample stage is located outside the machine body, so that gel cutting is conveniently performed outside the machine body to avoid ultraviolet rays.

Description

Technical Field

[0001] This invention belongs to the field of detection instruments, specifically a gel imaging analysis device that integrates imaging and analysis. Background Technology

[0002] A gel imaging system is a laboratory instrument used to observe, photograph, cut, and analyze the electrophoresis results of nucleic acids, proteins, etc. It can perform qualitative and semi-quantitative analysis of the separation and purification results of samples and is widely used in routine biological research such as molecular weight calculation, density scanning, and density quantification.

[0003] Currently, all gel imaging instruments on the market, whether imported or domestic brands, require the gel cutting process for nucleic acid electrophoresis to be performed with the ultraviolet lamp on. The operator places their hand inside the instrument and cuts the gel through the observation window. This inevitably exposes the operator's skin to ultraviolet radiation. Medical research has proven that ultraviolet radiation is most harmful to human skin and eyes. Prolonged exposure to ultraviolet radiation can cause wrinkles, spots, and dermatitis, and in severe cases, can lead to skin cancer, endangering human life and health. In addition, prolonged exposure of nucleic acid samples to ultraviolet radiation can damage the structure of nucleic acids, thus affecting the experimental results. Summary of the Invention

[0004] In view of the above situation and to overcome the shortcomings of the prior art, the present invention provides a gel imaging analysis device that integrates imaging and analysis, which effectively solves the problem that the operator's hands are easily damaged by long-term exposure to ultraviolet radiation during gel cutting, and that long-term ultraviolet radiation can easily destroy the structure of nucleic acids in the gel.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a gel imaging analysis device integrating imaging and analysis, comprising a body, wherein support feet are fixedly installed at the bottom of the body near the four corners, a connecting shaft is fixedly installed in the inner cavity of the body, an organic cover is rotatably connected to the outer side of the connecting shaft, a worktable is fixedly installed on the right side of the organic cover, a sample stage is fixedly installed on the top of the worktable, sliding grooves are provided on both sides of the worktable, a gel clamping mechanism is installed at the bottom of the worktable, an organic cover connecting mechanism is installed in the inner cavity of the body and is connected to the organic cover, an organic cover fixing mechanism is installed on the left side of the organic cover and is connected to the body;

[0006] The cover connecting mechanism includes two crossbars fixed to the inner cavity of the machine body. A sliding plate is movably installed on the outer side of the two crossbars. A rotating plate is rotatably connected to both sides of the front of the sliding plate. A fixed plate is rotatably connected to one side of the two rotating plates. The left side of the fixed plate is fixedly connected to the right side of the cover.

[0007] Preferably, an installation plate is fixedly installed in the inner cavity of the machine body, and a crossbar is fixedly installed on the back of the installation plate. One end of the crossbar is fixedly connected to the back of the inner cavity of the machine body. A slider is movably installed on the outer side of the crossbar. A rotating plate is rotatably connected to the left side of the slider. The left side of the rotating plate is rotatably connected to the right side of the slide plate.

[0008] Preferably, a spring is connected to the back of the slider, one side of the spring is connected to the back of the inner cavity of the machine body, and the spring is movably sleeved with the crossbar.

[0009] Preferably, the cover fixing mechanism includes a mounting rod fixed to the left side of the cover, a limit block fixedly connected to the left end of the mounting rod, a rotating cylinder rotatably connected to the outer side of the mounting rod, a movable rod movably mounted on the inner side of the rotating cylinder, a mounting block fixedly connected to one end of the movable rod, and a positioning rod fixedly connected to one side of the mounting block.

[0010] Preferably, a spring three is connected between the rotating cylinder and the mounting block, the spring three is movably sleeved with the movable rod, and a support plate is fixedly connected to the left side of the cover, with the top of the support plate fitting against the bottom of the rotating cylinder.

[0011] Preferably, a limiting plate is fixedly installed on the front of the machine body, a rubber pad is fixedly installed on the right side of the limiting plate, the right side of the rubber pad is in contact with the left side of the machine cover, a positioning hole is opened on the front of the limiting plate, and one end of the positioning rod extends to the inside of the positioning hole.

[0012] Preferably, the gel clamping mechanism includes a base rod fixed to the bottom of the workbench, a base block fixedly connected to the bottom end of the base rod, a movable block three movably mounted on the outside of the base block, a spring two connected between the movable block three and the workbench, and the spring two movably sleeved with the base rod.

[0013] Preferably, a fixed rod is fixedly connected to the inner side of each of the two slides, and a movable block is movably installed on the outer side of each of the two fixed rods. A rotating rod is rotatably connected to the bottom of each of the two movable blocks. The bottom ends of the two rotating rods are rotatably connected to the two sides of the movable block. A clamping plate is fixedly installed on the top of each of the two movable blocks, and the side of the two clamping plates that are close to each other is located on the top of the sample stage.

[0014] Preferably, side rods are fixedly connected to both sides of the base block, and side blocks are fixedly connected to the ends of the two side rods that are far apart from each other. Movable blocks II are movably installed on the outer sides of the two side rods. Bending rods are fixedly installed at the bottom of the two movable blocks II. Rotating rods II are rotatably connected to the top of the two movable blocks II. The tops of the two rotating rods II are rotatably connected to the two sides of the movable block III, respectively.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] (1) In this invention, through the cooperation between the crossbar 2, the slider and the spring 1, when the control of the machine cover is released, the originally stretched spring 1 is reset, causing the slider to move backward. Through the cooperation between the slider and the rotating plate 2, the sliding plate and the crossbar 1, the sliding plate moves to the left along the crossbar 1. Through the cooperation between the rotating plate 1 and the fixed plate, the machine cover and the connecting shaft, the machine cover rotates along the connecting shaft, thereby enabling the machine cover to rotate and the sample stage to be located on the outside of the machine body, which facilitates the cutting of glue on the outside of the machine body and avoids the exposure of ultraviolet rays.

[0017] (2) The invention enables the positioning rod to move and approach the rotating cylinder through the cooperation between the mounting block, the spring three, and the rotating cylinder, thereby compressing the spring three. Through the cooperation between the rotating cylinder and the mounting rod, the positioning rod can be rotated to the front of the positioning hole. When the spring three is reset, the mounting block can drive the positioning rod to move, thereby enabling one end of the positioning rod to be located inside the positioning hole, which facilitates fixing the position of the cover and prevents the cover from shaking when cutting the glue on the sample stage.

[0018] (3) The invention utilizes the cooperation between the bending rod and the movable block 2, as well as the side rod and the rotating rod 2. When the two bending rods are held, the movable block 3 can move upward along the bottom rod, and the spring 2 can be compressed. Through the cooperation between the rotating rod 1, the movable block 1, and the fixed rod, the two clamping plates move away from each other. When the control of the two bending rods is released, the spring 2 resets, causing the movable block 3 to move downward and the two clamping plates to move closer to each other, thereby facilitating the clamping of the gel located at the top of the sample stage, which facilitates subsequent gel cutting. Attached Figure Description

[0019] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof.

[0020] In the attached diagram:

[0021] Figure 1 This is a schematic diagram of the structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the cover structure of the present invention;

[0023] Figure 3 This is a schematic diagram of the cover connection mechanism of the present invention;

[0024] Figure 4 This is a schematic diagram of the cover fixing mechanism of the present invention;

[0025] Figure 5 This is a schematic diagram of the limiting plate structure of the present invention;

[0026] Figure 6 This is a schematic diagram of the gel clamping mechanism of the present invention.

[0027] In the diagram: 1. Body; 2. Cover connecting mechanism; 201. Crossbar 1; 202. Fixing plate; 203. Slide plate; 204. Rotating plate 1; 205. Mounting plate; 206. Crossbar 2; 207. Spring 1; 208. Slider; 209. Rotating plate 2; 3. Gel clamping mechanism; 301. Base rod; 302. Spring 2; 303. Movable block 1; 304. Rotating rod 1; 305. Rotating rod 2; 306. Movable block 2; 307. Side rod; 308. Base block; 309. Bending rod 3010, Side block; 3011, Movable block three; 3012, Fixed rod; 3013, Clamping plate; 4, Worktable; 5, Support foot; 6, Machine cover fixing mechanism; 601, Mounting rod; 602, Rotating cylinder; 603, Limiting plate; 604, Mounting block; 605, Limiting block; 606, Support plate; 607, Positioning hole; 608, Rubber pad; 609, Positioning rod; 6010, Movable rod; 6011, Spring three; 7, Machine cover; 8, Connecting shaft; 9, Slide groove; 10, Sample stage. Detailed Implementation

[0028] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0029] Example 1, by Figures 1-6 The present invention includes a body 1, with support feet 5 fixedly installed at the four corners of the bottom of the body 1. A connecting shaft 8 is fixedly installed in the inner cavity of the body 1, and an organic cover 7 is rotatably connected to the outer side of the connecting shaft 8. A worktable 4 is fixedly installed on the right side of the organic cover 7, and a sample stage 10 is fixedly installed on the top of the worktable 4. Slide grooves 9 are provided on both sides of the worktable 4. A gel clamping mechanism 3 is installed at the bottom of the worktable 4. An organic cover connecting mechanism 2 is installed in the inner cavity of the body 1 and is connected to the organic cover 7. An organic cover fixing mechanism 6 is installed on the left side of the organic cover 7 and is connected to the body 1.

[0030] In Embodiment Two, based on Embodiment One, the cover connecting mechanism 2 includes two crossbars 201 fixed to the inner cavity of the body 1. A sliding plate 203 is movably mounted on the outer side of the two crossbars 201. Rotating plates 204 are rotatably connected to both sides of the front of the sliding plate 203. A fixed plate 202 is rotatably connected to one side of each rotating plate 204. The left side of the fixed plate 202 is fixedly connected to the right side of the cover 7. An mounting plate 205 is fixedly installed in the inner cavity of the body 1. A crossbar 206 is fixedly installed on the back. One end of the crossbar 206 is fixedly connected to the back of the inner cavity of the machine body 1. A slider 208 is movably installed on the outside of the crossbar 206. A rotating plate 209 is rotatably connected to the left side of the slider 208. The left side of the rotating plate 209 is rotatably connected to the right side of the slide plate 203. A spring 207 is connected to the back of the slider 208. One side of the spring 207 is connected to the back of the inner cavity of the machine body 1. The spring 207 is movably sleeved with the crossbar 206.

[0031] When the control of the cover 7 is released, the originally stretched spring 207 resets, causing the slider 208 to move backward along the crossbar 206. The rotating plate 209 causes the slide plate 203 to move to the left along the two crossbars 201. At this time, the two rotating plates 204 drive the fixed plate 202 to move, and cause the cover 7 to rotate clockwise around the connecting shaft 8. This causes the sample stage 10 to move, so that the sample stage 10 is located outside the body 1. The cutting process can be completed on the sample stage 10, avoiding the exposure of the ultraviolet rays on the body 1.

[0032] In Embodiment 3, based on Embodiment 1, the hood fixing mechanism 6 includes a mounting rod 601 fixed to the left side of the hood 7. A limit block 605 is fixedly connected to the left end of the mounting rod 601. A rotating cylinder 602 is rotatably connected to the outer side of the mounting rod 601. A movable rod 6010 is movably mounted on the inner side of the rotating cylinder 602. A mounting block 604 is fixedly connected to one end of the movable rod 6010. A positioning rod 609 is fixedly connected to one side of the mounting block 604. A spring is connected between the rotating cylinder 602 and the mounting block 604. Spring 3 6011 is movably connected to movable rod 6010. Support plate 606 is fixedly connected to the left side of machine cover 7. The top of support plate 606 is attached to the bottom of rotating cylinder 602. Limit plate 603 is fixedly installed on the front of machine body 1. Rubber pad 608 is fixedly installed on the right side of limit plate 603. The right side of rubber pad 608 is attached to the left side of machine cover 7. Positioning hole 607 is opened on the front of limit plate 603. One end of positioning rod 609 extends to the inside of positioning hole 607.

[0033] When the cover 7 is opened, the left side of the cover 7 is in contact with the right side of the rubber pad 608. First, the mounting block 604 is moved, which drives the positioning rod 609 to move, so that the mounting block 604 is close to the rotating cylinder 602. At this time, the spring 6011 is compressed. Then, the rotating cylinder 602 is rotated through the mounting block 604, so that the mounting block 604 is located in front of the limiting plate 603. Then, the control of the mounting block 604 is released. At this time, the spring 6011 is reset, which drives the mounting block 604 to move away from the rotating cylinder 602, and drives the positioning rod 609 to move until one end of the positioning rod 609 is located inside the positioning hole 607. At this time, the cover 7 is fixed to the limiting plate 603, and the position of the cover 7 is fixed, thereby fixing the position of the sample stage 10 and preventing the cover 7 from shaking when cutting the glue.

[0034] In Example 4, based on Example 1, the gel clamping mechanism 3 includes a bottom rod 301 fixed to the bottom of the worktable 4. A bottom block 308 is fixedly connected to the bottom end of the bottom rod 301. A movable block 3011 is movably installed on the outer side of the bottom block 308. A spring 2 302 is connected between the movable block 3011 and the worktable 4. The spring 2 302 is movably sleeved with the bottom rod 301. Fixed rods 3012 are fixedly connected to the inner sides of the two sliding grooves 9. Movable blocks 1 303 are movably installed on the outer sides of the two fixed rods 3012. Rotating rods 1 304 are rotatably connected to the bottom of the two movable blocks 1 303. The bottom ends of the two rotating rods 1 304 are respectively connected to the movable blocks 3011, ... The two sides of 3011 are rotatably connected. The top of the two movable blocks 303 is fixedly installed with clamping plates 3013. The side of the two clamping plates 3013 that are close to each other is located on the top of the sample stage 10. The two sides of the bottom block 308 are fixedly connected with side rods 307. The two side rods 307 that are far apart from each other are fixedly connected with side blocks 3010. The outer sides of the two side rods 307 are movably installed with movable blocks 306. The bottom of the two movable blocks 306 is fixedly installed with bending rods 309. The top of the two movable blocks 306 is rotatably connected with rotating rods 305. The top of the two rotating rods 305 is rotatably connected to the two sides of the movable block 3011 respectively.

[0035] When cutting the gel, first hold the two bending rods 309 and move them closer together, which in turn moves the two movable blocks 306 closer together. At this time, the two rotating rods 305 move the movable block 3011 upward, while the spring 302 is compressed. Then, the two rotating rods 304 move the two movable blocks 303 away from each other, and the two clamping plates 3013 move away from each other. Then, place the gel on the top of the sample stage 10, so that it is between the two clamping plates 3013. Then release the control of the two bending rods 309. At this time, the spring 302 resets and moves the movable block 3011 downward, which in turn moves the two movable blocks 303 closer together. At the same time, the two clamping plates 3013 move closer together and clamp the gel, thus completing the fixation of the gel, which is convenient for subsequent cutting.

Claims

1. A gel imaging analysis device integrating imaging and analysis, comprising a body (1), characterized in that: The bottom of the machine body (1) is fixedly provided with support feet (5) near the four corners, the inner cavity of the machine body (1) is fixedly provided with a connecting shaft (8), the outer side of the connecting shaft (8) is rotatably connected to a cover (7), a workbench (4) is fixedly provided on the right side of the cover (7), a sample table (10) is fixedly provided on the top of the workbench (4), slide grooves (9) are provided on both sides of the workbench (4), a gel clamping mechanism (3) is installed on the bottom of the workbench (4), a cover connecting mechanism (2) is installed in the inner cavity of the machine body (1), the cover connecting mechanism (2) is connected to the cover (7), a cover fixing mechanism (6) is installed on the left side of the cover (7), and the cover fixing mechanism (6) is connected to the machine body (1); The machine cover connection mechanism (2) comprises two cross bars (201) fixed to the inner cavity of the machine body (1); a slide plate (203) is movably mounted on the outer sides of the two cross bars (201); both sides of the front side of the slide plate (203) are rotatably connected to a rotating plate (204); one side of the two rotating plates (204) is rotatably connected to a fixed plate (202); and the left side of the fixed plate (202) is fixedly connected to the right side of the machine cover (7); The gel clamping mechanism (3) comprises a bottom rod (301) fixed to the bottom of a workbench (4); the bottom end of the bottom rod (301) is fixedly connected to a bottom block (308); a movable block (3011) is movably mounted on the outer side of the bottom block (308); a spring (302) is connected between the movable block (3011) and the workbench (4); the spring (302) is movably sleeved with the bottom rod (301); the inner sides of the two slide grooves (9) are fixedly connected to fixed rods (3012); the outer sides of the two fixed rods (3012) are movably mounted to movable blocks (303); the bottoms of the two movable blocks (303) are rotatably connected to rotating rods (304); the bottom ends of the two rotating rods (304) are respectively connected to the movable block (3011); The two sides of the bottom block (308) are rotatably connected, the tops of the two movable blocks (303) are fixedly installed with clamping plates (3013), the sides of the two clamping plates (3013) close to each other are located on the top of the sample table (10), the two sides of the bottom block (308) are fixedly connected with side rods (307), the ends of the two side rods (307) away from each other are fixedly connected with side blocks (3010), the outer sides of the two side rods (307) are movably installed with movable blocks (306), the bottoms of the two movable blocks (306) are fixedly installed with bending rods (309), the tops of the two movable blocks (306) are rotatably connected with rotating rods (305), and the tops of the two rotating rods (305) are rotatably connected to the two sides of the movable block (3011).

2. The gel imaging analysis device integrating imaging and analysis according to claim 1, characterized in that: The inner cavity of the machine body (1) is fixedly mounted with a mounting plate (205), the back of the mounting plate (205) is fixedly mounted with a second cross bar (206), one end of the second cross bar (206) is fixedly connected to the back of the inner cavity of the machine body (1), a slider (208) is movably mounted on the outer side of the second cross bar (206), the left side of the slider (208) is rotatably connected with a second rotating plate (209), and the left side of the second rotating plate (209) is rotatably connected to the right side of the slide plate (203).

3. The gel imaging analysis device integrating imaging and analysis according to claim 2, characterized in that: The back of the slider (208) is connected to a spring 1 (207), one side of the spring 1 (207) is connected to the back of the inner cavity of the machine body (1), and the spring 1 (207) is movably sleeved with the crossbar 2 (206).

4. The imaging and analysis integrated gel imaging analysis device according to claim 1, characterized in that: The machine cover fixing mechanism (6) comprises a mounting rod (601) fixed to the left side of the machine cover (7); the left end of the mounting rod (601) is fixedly connected to a limiting block (605); the outer side of the mounting rod (601) is rotatably connected to a rotating cylinder (602); the inner side of the rotating cylinder (602) is movably mounted with a movable rod (6010); one end of the movable rod (6010) is fixedly connected to a mounting block (604); and one side of the mounting block (604) is fixedly connected to a positioning rod (609).

5. The imaging and analysis integrated gel imaging analysis device according to claim 4, characterized in that: A spring three (6011) is connected between the rotating cylinder (602) and the mounting block (604), and the spring three (6011) is movably sleeved with the movable rod (6010). A support plate (606) is fixedly connected to the left side of the machine cover (7), and the top of the support plate (606) is in contact with the bottom of the rotating cylinder (602).

6. The gel imaging analysis device integrating imaging and analysis according to claim 1, characterized in that: A limiting plate (603) is fixedly mounted on the front of the machine body (1); a rubber pad (608) is fixedly mounted on the right side of the limiting plate (603); the right side of the rubber pad (608) is in contact with the left side of the machine cover (7); a positioning hole (607) is provided on the front of the limiting plate (603); and one end of a positioning rod (609) extends to the inner side of the positioning hole (607).

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