Bactericidal composition containing glucan and bromothionil

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a bactericidal composition containing glucan sugar and bromothionil. Provided is a bactericidal composition containing glucan sugar and bromothionil, wherein the effective components are binary compounded with glucan sugar and bromothionil. When the two effective components of glucan sugar and bromothionil in the bactericidal composition of the present invention are compounded in a certain mass ratio, there is no cross-resistance, and a good synergistic effect is exhibited.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and specifically relates to a bactericidal composition containing glucanol and bromothionil. The invention also relates to the application of the bactericidal composition in agriculture. Background Art

[0002] Citrus canker is a bacterial disease that seriously harms citrus. The pathogen of citrus canker is Xanthomonas citrus, a member of the genus Xanthomonas of the phylum Thincicles. In recent years, the disease has occurred and spread to varying degrees in Yunnan, Guangxi, Guangdong, Hainan, Taiwan, Fujian, Zhejiang, Jiangsu, Hubei, Hunan, Jiangxi, and parts of Sichuan and Guizhou. It is easy to spread and difficult to control, and has always been a problem in citrus production. Chemical control is mainly used.

[0003] Bromothalonil is a broad-spectrum, highly effective, low-toxic fungicide that can inhibit and eradicate the growth of fungi, bacteria, and algae. It has a good preventive and therapeutic effect on crop diseases and is particularly effective against anthracnose. It is widely used in a variety of crops, including fruit trees, grapes, vegetables, cotton, peanuts, watermelons, tobacco, tea trees, and flowers, to prevent and control anthracnose, black spot, scab, powdery mildew, rust, damping-off, sudden wilt, root and stem rot, canker, bacterial wilt, angular leaf spot, and other fungal and bacterial diseases.

[0004] Bromothionil used alone has a good preventive effect on citrus canker. If this active ingredient is applied alone for a long time, the disease will easily develop drug resistance.

[0005] Glucan is a new type of plant resistance inducer. It can activate plant surface receptors and signal molecule conduction, regulate the production of plant pathogen-related proteins, phytoalexins and other related disease-resistant substances and the accumulation of secondary metabolites, prevent pathogens from infiltrating and spreading, and reduce the occurrence of crop diseases. At the same time, Glucan can activate plant cells, promote growth, and enhance the disease resistance of plants.

[0006] In the prior art, there is no report on the compounding of dextran sugar and bromothionil. Summary of the invention

[0007] The object of the present invention is to provide a fungicide composition containing glucanol and bromothionil, so as to overcome the disadvantage that long-term single application of bromothionil causes the disease to develop drug resistance.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] The present invention aims to provide a bactericidal composition containing glucan sugar and bromothionil, wherein the effective component is formed by a binary compound of glucan sugar and bromothionil.

[0010] The mass ratio of the glucanose to bromothionil is 1:200 to 5:1; preferably, the mass ratio is 1:40 to 2:5.

[0011] The invention provides a fungicide composition containing glucanol and bromothionil, which has good prevention and treatment effect on citrus canker.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] (1) When the two active ingredients of glucanose and bromothionil in the fungicide composition of the present invention are compounded in a certain mass ratio, there is no cross-resistance and a good synergistic effect is exhibited.

[0014] (2) The fungicidal composition of the present invention, wherein the dextran sugar is a biological sugar pesticide, is compounded with bromothionil, which improves the prevention effect of citrus canker while significantly reducing the application dosage of chemical pesticides and reducing pesticide residues. DETAILED DESCRIPTION

[0015] The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0016] Example: Activity test of fungicidal composition containing glucanol and bromothionil on indoor potted plants

[0017] Test pathogen: Citrus canker was isolated from diseased leaves of navel orange in Ganzhou, Jiangxi Province. The strain was isolated and purified by plate streaking method in beef extract peptone medium, and a single colony with waxy yellow color and metallic luster was obtained. It was inoculated on healthy leaves of navel orange in the greenhouse, and ulcer spots appeared, which was used to judge that the strain was navel orange canker pathogen. The purified strain was inoculated on the test tube slant medium, cultured at 26℃ for 48h, and then stored in the refrigerator for later use.

[0018] Test steps:

[0019] (1) Preparation of bacterial suspension: After the test bacteria were cultured on a slant medium for 40 h, sterile water was added and the bacterial moss was scraped off to prepare a bacterial suspension.

[0020] (2) Preparation of drugs: dissolve the dextran stock drug in distilled water and dissolve the bromothionil stock drug in methanol. After dissolution, dilute each drug with a 0.1% Tween-80 aqueous solution to form a mother liquor. The mother liquor is then prepared with a 0.1% Tween-80 aqueous solution into single and compound drugs with 5 concentration gradients.

[0021] (3) Chemical treatment: Spray the above chemicals evenly on the leaves until they are completely wet. Repeat 4 times for each treatment, with 5 pots for each repeat. Set a treatment without chemical as a blank control.

[0022] (4) Inoculation and cultivation: Inoculation was carried out 2 days after the treatment with the drug, and 10 leaves from the top of each plant were inoculated. After inoculation, the plants were cultivated at 26°C and 80% humidity.

[0023] (5) Disease investigation: Two weeks after inoculation, the disease status was checked on each leaf. The disease classification standards were as follows: Grade 0, no lesions; Grade 1, 1-5 lesions on each leaf; Grade 3, 6-10 lesions on each leaf; Grade 5, 11-15 lesions on each leaf; Grade 7, 16-20 lesions on each leaf; Grade 9, more than 21 lesions on each leaf.

[0024] (6) Data Statistical Analysis

[0025] According to the survey data, the disease index and control effect of each treatment were calculated.

[0026] Disease index = Σ[disease level × number of diseased plants at that level] / (total number of surveys × highest disease level) × 100

[0027] Relative efficacy (%) = (control disease index - treatment disease index) / control disease index × 100%

[0028] The EC of each agent was calculated by regression analysis based on the logarithmic value of each agent concentration and the corresponding control effect probability value. 50 The interaction of the mixture was evaluated according to the Wadley method, where SR = 0.5-1.5, the two drugs have an additive effect; SR ≤ 0.5, it is an antagonistic effect; SR ≥ 1.5, it is a synergistic effect.

[0029] EC 50 (theoretical value) = (a+b) / (a / EC 50a +b / EC 50b )

[0030] SR=EC 50 (Theoretical value) / EC 50 (Actual value)

[0031] Table 1 Results of indoor activity test of the composition containing glucanol and bromothionil against citrus canker pathogens

[0032]

[0033] It can be seen from Table 1 that the synergistic coefficients of glucan and bromothalonil in the mass ratio range of 1:200-5:1 are all greater than 1.5, indicating that the combination of glucan and bromothalonil has a synergistic effect on citrus canker pathogens, especially when the mass ratio is 1:40-2:5, the synergistic coefficients are all greater than 2.1, and the synergistic effect is significant.

[0034] The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different options and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A fungicidal composition containing glucanol and bromothionil, characterized in that: The effective component of the bactericidal composition is a binary compound of glucan sugar and bromothionil, and the mass ratio of the glucan sugar to bromothionil is 1:200 to 5:

1.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the dextran sugar to bromothionil is 1:40 to 2:

5.

3. Use of the fungicide composition according to any one of claims 1 to 2 in preventing and treating citrus canker.

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