A method and device for taming a host of a parasitic wasp in the pupal stage

Abstract

The present invention discloses a method for taming a host for a pupal parasitic wasp and a taming device thereof. The above-mentioned method for taming a host for a pupal parasitic wasp comprises the following steps: S1 making a taming device, providing a parasitic wasp breeding device, a plurality of hosts and their pupal parasitic wasps; S2 the parasitic wasps parasitize the host in the taming device; S3 removing the eclosion host in the taming device, then collecting the offspring of the parasitic wasps and transferring them to the parasitic wasp breeding device for breeding; S4 determining the parasitic selectivity of the parasitic wasps; S5 evaluating the parasitic ability of the parasitic wasps; S6 evaluating the taming effect of the parasitic wasp host. The present invention establishes a scientific taming system for continuous succession breeding, scientific comparison of biological parameters of parasitic wasps of different generations, and effective evaluation of taming effects, which can successfully taming parasitic wasp hosts, and cultivate parasitic wasp varieties with higher selectivity and parasitism rate for new hosts. At the same time, the eclosion hosts that have not been parasitized can be automatically and timely cleaned to prevent them from spreading everywhere in the environment.

Description

Technical Field

[0001] The invention relates to the field of entomology application technology, in particular to a method for taming a host of a pupal parasitic wasp, and also to a taming device used when implementing the taming method. Background Art

[0002] As a biological control factor, parasitic wasps play an important role in integrated pest management. When used in the field, parasitic wasps can reduce the use of chemical pesticides, thereby protecting the ecological environment and promoting the development of sustainable agriculture. Parasitic wasp host domestication refers to the continuous subgeneration of parasitic wasps on new specific hosts to improve the selectivity and parasitism ability of parasitic wasps on new hosts. It is an important way to use parasitic wasps for biological control research. Through parasitic wasp host domestication, parasitic wasp varieties with higher selectivity and parasitism rates for new hosts can be cultivated, making them effective biological control tools for these specific pests. It can also expand the scope of application of parasitic wasp biological control and be more widely used in field production. Especially in the process of preventing and controlling invasive organisms, domesticating parasitic wasps to parasitize invasive species has great application potential.

[0003] At present, there have been many studies on the devices and methods for parasitic wasps to parasitize hosts, but the parasitic process of parasitic wasp host domestication has its own particularity. First, host domestication is a long process, which not only requires continuous subgeneration of parasitic wasps, but also requires comparison of parasitic wasp biological parameters in each generation or every few generations to evaluate the domestication effect. The current related devices and methods do not involve the evaluation of biological parameters between generations of parasitic wasps; second, in the first few generations of host domestication, the parasitic wasps have extremely low parasitic ability on new hosts, and a large number of hosts provided to parasitic wasps are not effectively parasitized. These unparasitized hosts will eventually emerge as adults. In mild cases, they will spread in the insect breeding container, which is difficult to clean and affects the efficiency of insect breeding. In severe cases, they will spread everywhere in the indoor environment, causing an uncontrollable situation. Moreover, once they spread outside the laboratory, they will cause the risk of biological invasion, damage the local ecosystem, and seriously affect biological safety. At this stage, the parasitic device for parasitic wasps does not take the above problems into consideration. Summary of the invention

[0004] In view of the shortcomings of the prior art mentioned above, the purpose of the present invention is to provide a method and a domestication device for host domestication of pupal parasitic wasps to establish a scientific domestication system for continuous succession rearing of parasitic wasps, scientifically compare the biological parameters of parasitic wasps of different generations, and effectively evaluate the domestication effect, so as to successfully domesticate parasitic wasps and cultivate parasitic wasp varieties with higher selectivity and parasitism rate for new hosts. At the same time, the unparasitized emerged hosts can be automatically and timely cleaned to prevent them from spreading everywhere in the environment.

[0005] A method for taming a host of a parasitic wasp in the pupal stage comprises the following steps:

[0006] S1 makes a domestication device, provides a parasitic wasp rearing device, several hosts and their pupal stage parasitic wasps for standby use;

[0007] S2 parasitic wasps parasitize hosts in the acclimation device;

[0008] S3 removes the emerged hosts in the acclimation device, then collects the offspring of the parasitic wasps and transfers them to the parasitic wasp breeding device for breeding;

[0009] S4 uses a Y-tube to determine the parasitic selectivity of parasitic wasps;

[0010] S5 evaluates the parasitic ability of parasitic wasps by calculating the parasitic success rate;

[0011] S6 evaluates the host acclimation effect of parasitic wasps.

[0012] In one embodiment, the taming device includes a taming box, a top cover I, a top cover II, two groups of tubes inserted on the top cover I, air holes penetrating the wall of the taming box, and a sponge plug for blocking the air holes; the top cover I or the top cover II movable cover is arranged at the mouth of the taming box, a sticky insect board is pasted on the inner surface of the top cover II, and the sticky insect board is located in the taming box; the parasitic bee breeding device adopts a fruit fly tube, and the tube mouth of the fruit fly tube is filled with absorbent cotton dipped in 20% sucrose water.

[0013] Furthermore, the method for making the taming device is specifically performed as follows:

[0014] S11 Cut off 1 cm of the bottom of the centrifuge tube to make a cannula, and insert absorbent cotton dipped in 20% sucrose water into the lower part of the cannula;

[0015] S12 uses an electric soldering iron to solder two sockets on the top cover 1, and inserts the insert into the top cover 1 through the sockets;

[0016] S13 used an electric soldering iron to burn two ventilation holes on the acclimation box and inserted sponge plugs into the ventilation holes.

[0017] Furthermore, the host and the parasitic wasp are sequentially sent into the domestication device, and the parasitic wasp parasitizes the host in the domestication device; the parasitic wasp parasitizes the host in the domestication device, and the specific process is as follows: the host is placed in the domestication box, the top cover I is covered, the parasitic wasp is transferred to the domestication box along the air hole, and then a sponge plug is plugged to allow the parasitic wasp to fully parasitize the host.

[0018] In one of the embodiments, in S3, the sponge plug is pulled out, the parasitic bees are taken out along the air holes and transferred to a parasitic bee breeding device for breeding; the method for removing the emerged hosts in the taming device has the following specific operation process: after taking out the parasitic bees and transferring them, the top cover I is replaced with the top cover II and is placed on the taming box. After all the emerged host adults are stuck by the sticky insect board, the top cover II is removed to remove the emerged hosts to prevent them from escaping and spreading.

[0019] In one embodiment, the method for determining the parasitic selectivity of a parasitic wasp is specifically performed as follows:

[0020] The two side arms of the S41Y tube are connected to 60ml volumetric flasks with Telfon tubes, one of which is filled with an odor source, and the other is set as a blank or another odor source;

[0021] The parasitic bee S42 to be tested was introduced from the mouth of the long arm of the Y-shaped tube. Timing began 2 cm after it crawled over the mouth of the long arm. The parasitic bee made a choice at the bifurcation of the Y-shaped tube and went to different side arms. It was observed for 5 minutes. If it crawled 3 cm past the side arm and stayed for more than 15 seconds, it was marked as having chosen the odor, otherwise it was marked as having no choice of the odor.

[0022] In one of the embodiments, the parasitic success rate is calculated as follows: parasitic success rate (%) = number of parasitic wasps that have emerged / total number of hosts × 100%.

[0023] Furthermore, in S4, the parasitism selectivity measurement of undomesticated parasitoids was added as a control group; in S5, the parasitism success rate calculation of undomesticated parasitoids was added as a control group; S6 compared the differences in parasitism selectivity and parasitism ability between undomesticated parasitoids and parasitoids of different generations after domestication to evaluate the domestication effect.

[0024] A domestication device, comprising a domestication box, a top cover I, a top cover II, two sets of insert tubes inserted on the top cover I, air holes penetrating the wall of the domestication box, and a sponge plug for plugging the air holes;

[0025] The top cover I is provided with a through hole for inserting and positioning the cannula, the two groups of the cannula are arranged horizontally, and the cannula is plugged with absorbent cotton dipped in 20% sucrose water; there are two groups of air holes, the positions of the two groups of air holes are opposite, and the sponge plug is detachably mounted on the acclimation box.

[0026] In one embodiment, the top cover I or the top cover II movable cover is arranged at the opening of the acclimation box, and a sticky insect board is pasted on the inner surface of the top cover II, and the sticky insect board is located in the acclimation box;

[0027] When the top cover I is installed on the acclimation box, the acclimation device is used to provide a acclimation space for the parasitic wasp to parasitize the host; when the top cover II is installed on the acclimation box, the acclimation device is used to prevent the emerging host from escaping.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention improves the selectivity and parasitic ability of parasitic wasps to new hosts by domesticating parasitic wasps, thereby breeding parasitic wasp varieties with higher selectivity and parasitic rate to new hosts, making them effective biological control tools for these specific pests and expanding the application scope of biological control of parasitic wasps.

[0030] The present invention evaluates the domestication effect by evaluating the changes in host selection and parasitic ability of parasitic wasps of different domestication generations, thereby breeding parasitic wasp varieties with higher selectivity and parasitism rate for new hosts, making them effective biological control tools for these specific pests and expanding the application scope of parasitic wasp biological control.

[0031] The present invention establishes a scientific domestication system for continuously successive rearing of parasitic bees, scientifically comparing biological parameters of parasitic bees of different generations, and effectively evaluating domestication effects, which can successfully domesticate parasitic bees and cultivate parasitic bee varieties with higher selectivity and parasitism rate for new hosts. At the same time, the present invention can automatically and timely clean up the unparasitized emerged hosts to prevent them from spreading everywhere in the environment, thereby ensuring the stability and safety of the indoor rearing environment and reducing the ecological risks caused by host escape. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 Shown are the results of parasitism selectivity determination in a method for host domestication of pupal parasitic wasps provided in Example 1 of the present invention.

[0033] Figure 2 Shown are the test results of parasitism success rate in a method for host domestication of pupal parasitic wasps provided in Example 1 of the present invention.

[0034] Figure 3 Shown is a schematic structural diagram of a parasitic wasp breeding device in a method for host domestication of pupal parasitic wasps provided in Example 1 of the present invention.

[0035] Figure 4 Shown is a schematic structural diagram of a taming device provided by the present invention.

[0036] Figure 5 Shown Figure 4 In the figure, there is a schematic diagram of the matching of the taming box and the top cover I, in which the taming device is used to provide a taming space for the parasitic wasp to parasitize the host, and the arrow in the figure indicates the insertion direction of the absorbent cotton.

[0037] Figure 6 Shown Figure 4 In the figure, there is a schematic diagram of the cooperation between the acclimation box and the top cover II, in which the acclimation device is used to prevent the emerging host adults from escaping.

[0038] Main component symbols

[0039] 1. Acclimation box; 2. Top cover I; 3. Top cover II; 4. Intubation tube; 5. Air vent; 6. Sponge plug; 7. Fruit fly tube.

[0040] The above description of the main component symbols is combined with the accompanying drawings and specific implementation methods to further illustrate the present invention in detail. DETAILED DESCRIPTION

[0041] The present invention is described in detail below with reference to the accompanying drawings.

[0042] Example 1

[0043] This embodiment provides a method for taming a parasitic wasp in the pupal stage. It should be noted that this embodiment is described by taming a parasitic wasp, whose original host is a Drosophila melanogaster pupa, to parasitize a new host, Bactrocera dorsalis. The taming method includes the following steps:

[0044] S1: Prepare a domestication device, provide a parasitic wasp rearing device, and a number of 2-5-day-old Bactrocera dorsalis pupae and their pupal stage parasitic wasps (Psora chrysogenum) for standby use;

[0045] The taming device comprises a taming box 1 , a top cover I2 , a top cover II3 , two groups of insert tubes 4 inserted on the top cover I2 , an air vent 5 penetrating through the wall of the taming box 1 , and a sponge plug 6 for blocking the air vent 5 .

[0046] The top cover I2 or the top cover II3 movable cover is arranged at the box opening of the taming box 1, and the inner surface of the top cover II3 is pasted with an insect sticking board, and the insect sticking board is located in the taming box 1. Figure 5 When the top cover 12 is placed on the taming box 1, the golden wasp parasitizes the oriental fruit fly in the taming device. Figure 6 When the top cover II3 is set on the acclimation box 1, the sticky surface of the sticky insect board faces the bottom of the acclimation box 1, and the emerged adult citrus fruit fly is stuck to the sticky surface of the sticky insect board. At this time, the acclimation device plays a role in preventing the emerged adult citrus fruit fly from escaping.

[0047] The specific operation of the method for making the taming device is as follows:

[0048] S11: Cut off 1 cm of the bottom of the centrifuge tube to make a cannula 4, insert absorbent cotton soaked in 20% sucrose water into the lower part of the cannula 4, and the amount of sucrose water should be such that the absorbent cotton is soaked without dripping;

[0049] S12 uses an electric soldering iron to solder two sockets on the top cover I2, and inserts the plug 4 into the top cover I2 through the sockets;

[0050] S13 uses an electric soldering iron to solder two vent holes 5 on the taming box 1, and inserts sponge plugs 6 into the vent holes.

[0051] In this embodiment, an acclimation box 1 with a bottom diameter of 12.3 cm, a top diameter of 17 cm, and a height of 9 cm is used, and a top cover I2 and a top cover II3 with a diameter of 17.5 cm. The cannula 4 in this embodiment is obtained by cutting a centrifuge tube with a height of 4 cm, an inner diameter of 1 cm, and a capacity of 1.5 ml. A sponge plug 6 with a diameter of 2.6 cm and a height of 2.7 cm is taken as an example for explanation, wherein the height of the cannula 4 left outside the acclimation box 1 is 2 cm, the center of the air hole 5 is 4.5 cm away from the bottom of the acclimation box 1, the diameter of the air hole 5 is 2 cm, and the distance that the sponge plug 6 is inserted into the air hole 5 is half of its own height.

[0052] Please refer to Figure 3 The parasitic wasp breeding device adopts a fruit fly tube 7, and the tube mouth of the fruit fly tube 7 is filled with absorbent cotton dipped in 20% sucrose water. This embodiment takes a fruit fly tube 7 with a height of 9.5 cm and a diameter of 2.3 cm as an example for explanation. The sucrose water is provided for the golden bee to eat. It should be noted that the amount of sucrose water is based on the amount of sucrose water that soaks the absorbent cotton without dripping. In addition, in order to prevent the absorbent cotton from falling, the absorbent cotton is pressed and fixed at the tube mouth of the fruit fly tube 7 by using a tube plug to plug the tube mouth and squeeze the absorbent cotton at the same time.

[0053] S2 golden wasp parasitizes Bactrocera dorsalis in the acclimation device;

[0054] The orange fruit fly and the golden wasp are sequentially sent to the acclimation device, and the golden wasp parasitizes the orange fruit fly in the acclimation device. The golden wasp parasitizes the orange fruit fly in the acclimation device, and the specific process is as follows: the orange fruit fly is placed in the acclimation box 1, the top cover 12 is covered, the golden wasp is transferred to the acclimation box 1 along the air vent 5, and then the sponge plug 6 is plugged, and the acclimation device, the golden wasp and the orange fruit fly are placed together at a temperature of 25° C. and a relative humidity of 60% for 24 hours to allow the golden wasp to fully parasitize the orange fruit fly.

[0055] S3 removes the emerged Bactrocera dorsalis in the acclimation device, collects the offspring of the golden wasp and transfers them to the parasitic wasp breeding device for breeding;

[0056] In S3 of the present embodiment, the sponge plug 6 is pulled out, the golden wasp is taken out along the air vent 5 and transferred to the parasitic wasp breeding device for breeding, and the breeding conditions are a temperature of 25° C. and a relative humidity of 60%. The method for removing the emerged orange fruit flies in the acclimation device has the following specific operation process: the top cover I2 is replaced with the top cover II3 and is covered on the acclimation box 1, and after all the emerged orange fruit flies are stuck by the sticky insect board, the top cover II3 is removed to remove the emerged orange fruit flies and prevent them from escaping and spreading.

[0057] S4 used a Y-tube to determine the parasitic selectivity of the golden wasp;

[0058] This embodiment is described by taking a Y-shaped tube with a long arm of 7 cm, two side arms of 5 cm, an angle of 75° between the two side arms, an inner diameter of the long arm and the side arm of 1 cm, and a standard ground mouth at the intersection as an example. The method for determining the parasitic selectivity of the golden wasp is specifically performed as follows:

[0059] The two side arms of the S41Y tube are connected to 60ml volumetric flasks with Telfon tubes. The odor source is placed in one volumetric flask, and the other volumetric flask is set as a blank or another odor source. The volumetric flasks are connected to the distilled water humidification bottle and the air purification bottle in turn with tubes. The air flow rate is adjusted to 0.5-0.6cm / s, the measurement time is 08:00am-12:00am, and the measurement temperature is 25±1℃;

[0060] The S42 golden wasp to be tested was introduced from the mouth of the long arm of the Y-shaped tube. The timing started after it crawled 2 cm past the mouth of the long arm. The golden wasp made a choice at the bifurcation of the Y-shaped tube and went to different side arms. It was observed for 5 minutes. If it crawled 3 cm past the side arm and stayed for more than 15 seconds, it was marked as having chosen the odor, otherwise it was marked as having no choice of the odor.

[0061] In order to eliminate the possible influence of the position of the tube arms on the behavior of the golden wasps, in this embodiment, the positions of the two arms of the Y-tube were swapped every five heads measured. In addition, in order to eliminate the influence of residual odors between different treatments, the olfactometer was replaced after each treatment measurement, and the Y-tube and the tube and volumetric flask connected to the Y-tube were thoroughly cleaned with alcohol and dried naturally. The Y-tube and volumetric flask were dried at 100°C for 24 hours.

[0062] S5 evaluates the parasitic ability of the golden wasp by calculating the parasitic success rate;

[0063] The formula for calculating parasitic power generation is:

[0064] Parasitism success rate (%) = number of emerged golden wasps / total number of citrus fruit flies × 100%; It should be noted that in this embodiment, in S4, the parasitism selectivity determination of untamed golden wasps was added as a control group. In S5, the parasitism success rate calculation of untamed golden wasps was added as a control group.

[0065] S6 evaluates the host acclimation effect of the golden wasp;

[0066] Compare the differences in parasitic selectivity and parasitic ability between untamed golden wasps and golden wasps of different generations after domestication, evaluate the domestication effect, and provide a reliable basis for whether to continue domestication and subsequent experimental plans.

[0067] The parasitic selectivity measurement results of this example are as follows Figure 1 As shown, analysis Figure 1 It can be seen that the fly pupa golden wasp that has not been domesticated to the new host (citrus fruit fly pupa) tends to parasitize the original host, that is, the Drosophila melanogaster pupa ( Figure 1 A), after 24 generations of domestication with new hosts (citrus fruit fly pupae), the selectivity of the fly pupa wasp for the new host (citrus fruit fly pupae) was significantly improved ( Figure 1 B).

[0068] The parasitic power test results of this embodiment are as follows Figure 2 As shown, analysis Figure 2 It can be seen that by comparing the parasitism success rates of the undomesticated golden wasps and the 6th, 10th, 24th and 29th generations of golden wasps after taming, it can be seen that the parasitism success rate of the bees on the new host has been significantly improved since the 10th generation of host taming. The above results prove that the taming method of this embodiment greatly improves the parasitic selectivity and parasitic ability of the golden wasps on the new host, and the taming is successful. In addition, in order to increase the parasitism rate, it is necessary to taming for multiple generations continuously, and at least taming to the 10th generation can significantly improve the parasitism rate compared with the untamed bees.

[0069] The domestication method of this embodiment improves the selectivity and parasitic ability of parasitic wasps to new hosts by domesticating parasitic wasps, thereby breeding parasitic wasp varieties with higher selectivity and parasitic rate to new hosts, making them effective biological control tools for these specific pests and expanding the application scope of parasitic wasp biological control.

[0070] The domestication method of this embodiment evaluates the domestication effect by evaluating the changes in host selection and parasitic ability of parasitic wasps of different domestication generations, thereby breeding parasitic wasp varieties with higher selectivity and parasitism rate for new hosts, making them effective biological control tools for these specific pests and expanding the application scope of parasitic wasp biological control.

[0071] In summary, the domestication method of this embodiment has established a scientific domestication system for continuously raising parasitic bees, scientifically comparing the biological parameters of parasitic bees of different generations, and effectively evaluating the domestication effect. It can successfully domesticate parasitic bees and cultivate parasitic bee varieties with higher selectivity and parasitism rate for new hosts. At the same time, it can automatically and timely clean up the unparasitized emerged hosts to prevent them from spreading everywhere in the environment, thereby ensuring the stability and safety of the indoor breeding environment and reducing the ecological risks caused by host escape.

[0072] Example 2

[0073] See also Figure 4-6 This embodiment provides a taming device, which provides a taming space for a parasitic wasp to taming a host in a method for taming a parasitic wasp in the pupal stage as described in Example 1. The taming device includes a taming box 1, a top cover I2, a top cover II3, two sets of inserts 4 inserted on the top cover I2, an air vent 5 penetrating through the wall of the taming box 1, and a sponge plug 6 for blocking the air vent 5.

[0074] The top cover 12 is provided with a through hole for inserting and positioning the cannula 4. Two groups of cannula 4 are arranged horizontally, and the bottom of the centrifuge tube is cut off. The cannula 4 is plugged with absorbent cotton dipped in 20% sucrose water. There are two groups of air holes 5, which are located opposite to each other. The sponge plug 6 is detachably mounted on the acclimation box 1.

[0075] The top cover I2 or the top cover II3 movable cover is arranged at the box opening of the taming box 1, and a sticky insect board is pasted on the inner surface of the top cover II3, and the sticky insect board is located in the taming box 1. When the top cover I2 is covered on the taming box 1, the taming device is used to provide a taming space for the parasitic wasp to parasitize the host; when the top cover II3 is covered on the taming box 1, the taming device is used to prevent the escaping host from escaping.

[0076] The domestication device of the present embodiment is low-cost, safe and environmentally friendly, the required accessories are common and easily available, and the total price is less than RMB 20. At the same time, most of the accessories are made of reusable materials and can be used many times without losing performance. The manufacturing process is safe and environmentally friendly, and no pollutants are generated during use, and there is no environmental pollution problem. The domestication device of the present embodiment is easy to operate, requires few accessories, and has a simple assembly process. The installation can be completed within 5 minutes, and the disassembly process is not complicated. It is easy to store and clean. No superb skills or professional tools are required for use. Both professionals and ordinary users can easily use the device to perform related operations.

[0077] The naming of each component involved is based on its function described in the specification and is not limited to the specific terms used in the present invention. Those skilled in the art may also choose other terms to describe the names of the components of the present invention.

Claims

1. A method for taming a host of a pupal parasitic wasp, characterized in that: The following steps are involved: S1 makes a domestication device, provides a parasitic wasp rearing device, several hosts and their pupal stage parasitic wasps for standby use; The taming device comprises a taming box, a top cover I, a top cover II, two groups of cannulas inserted on the top cover I, air holes provided through the wall of the taming box, and a sponge plug for plugging the air holes; a plug hole for providing the cannulas for insertion and positioning is provided through the top cover I, two groups of cannulas are arranged horizontally, and absorbent cotton dipped in 20% sucrose water is provided inside the cannulas; there are two groups of air holes, the positions of the two groups of air holes are opposite, and the sponge plug is detachably mounted on the taming box; The top cover I or the top cover II movable cover is arranged at the opening of the taming box; when the top cover I is arranged on the taming box, the taming device is used to provide a taming space for the parasitic wasp to parasitize the host; when the top cover II is arranged on the taming box, a sticky insect board is pasted on the inner surface of the top cover II, and the sticky insect board is located in the taming box, and the taming device is used to prevent the escaping host from emerging; S2 parasitic wasps parasitize hosts in the acclimation device; S3 removes the emerged hosts in the acclimation device, then collects the offspring of the parasitic wasps and transfers them to the parasitic wasp breeding device for breeding; S4 uses a Y-tube to determine the parasitic selectivity of parasitic wasps; S5 evaluates the parasitic ability of parasitic wasps by calculating the parasitic success rate; S6 evaluates the host acclimation effect of parasitic wasps.

2. The method for host domestication of pupal parasitic wasps according to claim 1, characterized in that: The parasitic wasp breeding device adopts a fruit fly tube, and the tube mouth of the fruit fly tube is filled with absorbent cotton dipped in 20% sucrose water.

3. A method for host domestication of pupal parasitic wasps according to claim 2, characterized in that: The method for making the taming device is specifically performed as follows: S11 Cut off 1 cm of the bottom of the centrifuge tube to make a cannula, and insert absorbent cotton dipped in 20% sucrose water into the lower part of the cannula; S12 uses an electric soldering iron to solder two sockets on the top cover 1, and inserts the insert into the top cover 1 through the sockets; S13 used an electric soldering iron to burn two ventilation holes on the acclimation box and inserted sponge plugs into the ventilation holes.

4. The method for host domestication of pupal parasitic wasps according to claim 3, characterized in that: The host and the parasitic wasp are sequentially sent into the domestication device, and the parasitic wasp parasitizes the host in the domestication device; The parasitic bee parasitizes the host in the domestication device, and the specific process is as follows: the host is placed in the domestication box, the top cover I is covered, the parasitic bee is transferred to the domestication box along the air vents, and then a sponge plug is plugged to allow the parasitic bee to fully parasitize the host.

5. The method for host domestication of pupal parasitic wasps according to claim 4, characterized in that: In S3, the sponge plug is removed, the parasitic wasp is taken out along the air vent and transferred to a parasitic wasp breeding device for breeding; The method for removing the emerged hosts in the acclimation device has the following specific operation process: after the parasitic wasps are taken out and transferred, the top cover I is replaced with the top cover II which is placed on the acclimation box; after all the emerged host adults are stuck by the sticky insect board, the top cover II is removed to remove the emerged hosts and prevent them from escaping and spreading.

6. The method for host domestication of pupal parasitic wasps according to claim 1, characterized in that: The method for determining the parasitic selectivity of a parasitic wasp is specifically performed as follows: The two side arms of the S41Y tube are connected to 60ml volumetric flasks with Telfon tubes, one of which is filled with an odor source, and the other is set as a blank or another odor source; The parasitic bee S42 to be tested was introduced from the mouth of the long arm of the Y-shaped tube. Timing began 2 cm after it crawled over the mouth of the long arm. The parasitic bee made a choice at the bifurcation of the Y-shaped tube and went to different side arms. It was observed for 5 minutes. If it crawled 3 cm past the side arm and stayed for more than 15 seconds, it was marked as having chosen the odor, otherwise it was marked as having no choice of the odor.

7. The method for host domestication of pupal parasitic wasps according to claim 1, characterized in that: The calculation formula of the parasitic power generation is: Parasitism success rate (%) = number of emerged parasitic wasps / total number of hosts × 100%.

8. The method for host domestication of pupal parasitic wasps according to claim 7, characterized in that: In S4, the parasitism selectivity test of untamed parasitoids was added as a control group; in S5, the parasitism success rate calculation of untamed parasitoids was added as a control group; S6 compares the differences in parasitic selectivity and parasitic ability between untamed parasitic wasps and parasitic wasps of different generations after taming, and evaluates the taming effect.

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