A detection and cultivation device for bacteriophage viruses in soil

Abstract

The present invention relates to a detection and cultivation device for bacteriophage viruses in soil, and relates to the field of microbial cultivation devices, including a microbial culture box with a stage inside and a photographic detection unit arranged in the microbial culture box, and also includes a rotating rack 1 and a rotating rack 2 arranged concentrically on the stage, the rotating rack 2 is arranged to rotate back and forth and has a reciprocating transmission member on its outer side; a plurality of racks 1 for carrying culture dish bodies arranged on the rotating rack 1, a rack 2 for carrying culture dish covers is arranged above the rack 1, and position adjustment members are arranged at the ends of the racks 2. The detection and cultivation device provided by the present invention can optimize the operation steps, save part of the manual operation process, and further improve the detection and cultivation operation efficiency for bacteriophage viruses.

Description

Technical Field

[0001] The invention belongs to the field of microbial culture devices, and in particular relates to a detection and culture device for bacteriophage viruses in soil. Background Art

[0002] Current studies have shown that bacteriophages in the soil can not only "specifically hunt" and "precisely target" pathogens to reduce their ability to survive and compete, but can also readjust the structure of the rhizosphere soil flora, restore community diversity, and increase the abundance of antagonistic beneficial bacteria in the community. Therefore, studying bacteriophages in the soil has positive significance for improving the soil and balancing the soil microecology.

[0003] The cultivation of bacteriophages is the basis for studying bacteriophages. Commonly used methods for phage cultivation include solid culture, liquid culture, and filter membrane culture methods. Among them, the filter membrane culture method for soil phages is to add an appropriate amount of phage soil eluate to the culture medium containing host bacteria, filter the culture medium onto a filter membrane with an appropriate pore size, and place the filter membrane with phages and host bacteria trapped in the culture medium for cultivation. During the cultivation process, the phages will infect and kill the host bacteria, forming plaques on the filter membrane. The detection of phages is to explore the ability of phages to infect host bacteria based on the size and morphology of the plaques. In order to ensure that reliable and sufficient experimental data are provided for phage-related research, a sufficient number of treatment groups need to be set up in the actual detection and cultivation process. This leads to the problem that the operation efficiency is difficult to further improve due to the large amount of sample processing during the experiment. Summary of the invention

[0004] The purpose of the present invention is to provide a detection and cultivation device for bacteriophage viruses in soil in order to solve the above problems.

[0005] The present invention achieves the above-mentioned purpose through the following technical solutions:

[0006] The present invention provides a detection and cultivation device for bacteriophage viruses in soil, comprising a microbial culture box with a stage inside and a photographic detection unit arranged in the microbial culture box, characterized in that: it also comprises a rotating material frame 1 and a rotating material frame 2 which are arranged concentrically on the stage, the rotating material frame 2 is arranged to reciprocate and a reciprocating transmission member is arranged on the outer side thereof;

[0007] and a plurality of carrier racks 1 for carrying culture dish bodies arranged on the rotating material rack 1, a plurality of carrier racks 2 for carrying culture dish covers are arranged above the carrier rack 1, and position adjustment members are arranged at the ends of the carrier racks 2;

[0008] When the second rotating material rack reciprocates, the second carrier rack generates transmission through the position adjustment member and the reciprocating transmission member so that the second carrier rack generates repeated opening and closing actions relative to the first carrier rack to realize ventilation of the culture dish.

[0009] As a further optimization scheme of the present invention, the structure of the rotating material rack includes an annular conveyor belt, a gear transmission member that cooperates with the annular conveyor belt, and a driving structure that is used to drive the gear transmission member to drive the annular conveyor belt to rotate.

[0010] As a further optimization scheme of the present invention, the structure of the second rotating material rack includes an annular conveyor belt, a gear transmission component that cooperates with the annular conveyor belt, and a driving structure for driving the gear transmission component to drive the annular conveyor belt to reciprocate.

[0011] As a further optimization scheme of the present invention, the position adjustment component includes a second connecting shaft arranged at the two ends of the carrier, a connecting frame arranged at the two ends of the connecting shaft, and an adjustment seat arranged at the end of the connecting frame. A telescopic component is provided at the lower end of the connecting frame, and an elastic reset component is provided in the adjustment seat and is slidably connected with a protruding rod through the elastic reset component. The protruding rod is arranged above the reciprocating transmission component.

[0012] As a further optimization scheme of the present invention, the reciprocating transmission member includes a reciprocating track 1 arranged on the outside of the straight ends on both sides of the circular conveyor belt 2 and a reciprocating track 2 arranged on the outside of the curved end on one side of the circular conveyor belt 2, and a transmission rack is provided at the position corresponding to the reciprocating track 2 on the loading platform.

[0013] As a further optimization scheme of the present invention, the lower end of the position adjusting member is movably cooperated with a swing adjusting member, and the swing adjusting member includes a rotating shaft member arranged at one end of the carrier, a threaded sleeve adjusted at the end of the rotating shaft member, and a vertical rod connected to the position adjusting member arranged at the upper end of the threaded sleeve, and the threaded sleeve is in transmission cooperation with the transmission rack.

[0014] As a further optimization solution of the present invention, the first carrier includes a limiting seat, two connecting seats symmetrically arranged on the limiting seat, and an overflow groove opened on the connecting seat.

[0015] As a further optimization solution of the present invention, the second carrier rack is provided with a plurality of negative pressure grippers for grasping the culture dish cover.

[0016] The beneficial effects of the present invention are:

[0017] The present invention enables the second carrier to automatically flip relative to the first carrier while the first rotating material rack drives the first carrier to rotate through the arrangement of the swing adjustment member, the reciprocating transmission member and the position adjustment member, so that the operator can place the culture dish body and the culture dish cover conveniently and can complete the automatic closing of the culture dish body and the culture dish cover, optimize the operation steps, save part of the manual operation process, and further improve the operation efficiency.

[0018] The present invention realizes the reciprocating rotation of the rotating material rack 2, so that the specimen rack 2 can generate transmission through the position adjustment member and the reciprocating transmission member, and then the culture dish cover carried on the specimen rack 2 can generate repeated opening and closing actions relative to the culture dish body carried on the specimen rack 1 to complete the batch ventilation of the culture dishes, and the operating efficiency is further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the three-dimensional structure of the device provided by the present invention;

[0020] Figure 2 A top view of the internal structure of the sterile incubator provided by the present invention;

[0021] Figure 3 A schematic diagram of the overall structure of the rotary rack 1 and the rotary rack 2 provided by the present invention;

[0022] Figure 4 The installation structure diagram of the first and second carrier racks provided by the present invention;

[0023] Figure 5 The present invention provides Figure 4 Schematic diagram of the installation between the position adjustment member and the swing adjustment member at A in the middle.

[0024] Figure 6 A schematic diagram of the structure of the annular transmission belt 2 and the reciprocating transmission part provided by the present invention;

[0025] Figure 7 The present invention provides Figure 3 A magnified view of the structure of the middle B section;

[0026] In the figure: 1, microbial incubator; 2, loading platform; 3, rotating rack 1; 31, annular conveyor belt 1; 32, transmission gear 1; 33, transmission gear ring 1; 34, drive shaft 1; 35, meshing gear set; 4, loading rack 1; 41, limit seat; 42, connecting seat; 43, overflow trough; 5, loading rack 2; 6, rotating rack 2; 61, annular conveyor belt 2; 62, reciprocating transmission member; 621, reciprocating track 1; 622, reciprocating track 2; 63, transmission Ring gear 2; 64, transmission gear 2; 65, intermittent drive unit; 651, swing seat; 652, swing frame; 653, drive shaft 2; 654, connecting shaft 1; 7, position adjustment member; 71, connecting shaft 2; 72, connecting frame; 73, adjustment seat; 74, protruding rod; 75, elastic reset member; 76, telescopic member; 8, swing adjustment member; 81, rotating shaft member; 82, vertical rod; 83, threaded sleeve; 9, transmission rack; 10, negative pressure gripper; 11, photo detection unit. DETAILED DESCRIPTION

[0027] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0028] Example 1

[0029] like Figure 1-2 As shown, this embodiment provides a detection and cultivation device for bacteriophage viruses in soil, and the structure includes a microbial culture box 1 with a stage 2 inside and a photographing and detecting unit 11 arranged in the microbial culture box 1. The microbial culture box 1 is a microbial culture device well known to those skilled in the art, and can meet the control and regulation of temperature, gas and other related conditions required for the co-cultivation of bacteriophages and host bacteria. The photographing and detecting unit 11 arranged in the microbial culture box 1 is a common photographing device. When facing the plaque formation test for bacteriophage inspection, the photographing and detecting unit 11 photographs and records the morphology of the culture dish at the beginning and end of the cultivation, so as to facilitate the statistics and detection of the morphology, number and size of the plaques in the later stage.

[0030] The device also includes a rotating rack 1 3 and a rotating rack 2 6 which are concentrically arranged on the stage 2. The rotating rack 2 6 is arranged to rotate back and forth and a reciprocating transmission member 62 is arranged on the outside thereof. In this embodiment, the reciprocating transmission member 62 is a reciprocating track 1 621 arranged on the outside of the straight ends on both sides of the annular conveyor belt 2 61. To ensure its reciprocating property, the shape of the reciprocating track 1 621 is preferably set to be a waveform;

[0031] Furthermore, the structure of the rotating material rack 1 3 includes an annular conveyor belt 1 31, a gear transmission member 1 that cooperates with the annular conveyor belt 1 31 for transmission, and a driving structure 1 for driving the gear transmission member 1 to drive the annular conveyor belt 1 31 to rotate. In this embodiment, the driving structure 1 is specifically a driving motor that outputs a rotational motion. The structure of the rotating material rack 2 6 includes an annular conveyor belt 2 61, a gear transmission member 2 that cooperates with the annular conveyor belt 2 61 for transmission, and a driving structure 2 that drives the annular conveyor belt 2 61 to reciprocate in rotation with the driving gear transmission member 2. In this embodiment, the driving structure 2 is specifically a driving motor that outputs a reciprocating rotational motion.

[0032] Furthermore, if Figure 4 As shown, the structure of the device also includes a plurality of carrier racks 4 for carrying culture dish bodies arranged on a rotating material rack 3, a carrier rack 2 5 for carrying culture dish covers is arranged above the carrier rack 4, and a position adjustment member 7 is arranged at the end of the carrier rack 2 5.

[0033] Among them, the specimen rack 1 4 includes a limiting seat 41, two connecting seats 42 symmetrically arranged on the limiting seat 41, and an overflow groove 43 opened on the connecting seat 42. The overflow groove 43 can guide the air in the box to the connecting seat 42 in a directional manner to realize ventilation of the culture dish carried on the specimen rack 1 4. In addition, the specimen rack 2 5 is provided with a plurality of negative pressure grippers 10 for grasping the culture dish cover, which is convenient for grasping and fixing the culture dish cover.

[0034] Furthermore, if Figure 4-5 As shown, the position adjustment member 7 includes a connecting shaft 71 arranged at the end of the carrier 5, a connecting frame 72 arranged at the end of the connecting shaft 71 and an adjustment seat 73 arranged at the end of the connecting frame 72. The lower end of the connecting frame 72 is provided with a telescopic member 76 movably plugged with the swing adjustment member 8. An elastic reset member 75 is provided in the adjustment seat 73 and a convex rod 74 is slidably connected to the elastic reset member 75. The convex rod 74 is arranged above the reciprocating transmission member 62. The adjustment seat 73 is specifically provided with a limiting groove for the convex rod 74 to generate displacement, and its shape is adapted to the displacement trajectory generated by the adjustment seat 73.

[0035] When the rotating rack 2 6 reciprocates, the carrier rack 2 5 generates transmission through the position adjustment member 7 and the reciprocating transmission member 62, so that the culture dish cover carried on the carrier rack 2 5 generates a repeated opening and closing action relative to the culture dish body carried on the carrier rack 1 4 to complete the ventilation of the culture dish. Specifically:

[0036] When the culture dish needs to be ventilated, the rotating rack 26 is driven to reciprocate. When the rotating rack 26 reciprocates, its annular conveyor belt 261 produces reciprocating intermittent swings, and the reciprocating track 1621 on the straight end of the annular conveyor belt 261 contacts the protruding rod 74. When the annular conveyor belt 261 swings, the "peak position" of the reciprocating track 1621 moves to the position of the protruding rod 74. The protruding rod 74 is pushed by the reciprocating track 1621 and its position rises. The protruding rod 74 is adjusted through the adjusting seat 73 and the connecting frame 72. , the specimen rack 5 connected to the second coupling 71 rises as the position of the protruding rod 74 rises, thereby realizing the separation of the culture dish cover carried by the specimen rack 5 from the culture dish body, thereby generating a gap. Similarly, when the "trough position" of the reciprocating track 1 621 contacts the protruding rod 74 as the annular conveyor belt 2 61 swings, the protruding rod 74 is reset along with the protruding rod 74 through the adjusting seat 73, the connecting frame 72, and the specimen rack 5 connected to the second coupling 71. At this time, the culture dish cover on the specimen rack 5 covers the culture dish body again.

[0037] According to the above-mentioned action process, the culture dish cover carried on the second carrier 5 produces a repeated opening and closing action relative to the culture dish body carried on the first carrier 4, thereby completing the batch ventilation operation of the culture dishes with high operation efficiency.

[0038] Example 2

[0039] On the basis of Example 1, Figure 3 , 7 As shown, the gear transmission member 2 includes a transmission gear ring 63 arranged on the inner wall of the circular transmission belt 61, and a transmission gear 64 arranged at both ends of the circular transmission belt 61 and meshing with the transmission gear ring 63. The driving structure 2 in this embodiment is specifically an intermittent driving unit 65, and the intermittent driving unit 65 includes a swing seat 651 coaxially arranged with the transmission gear 64, two swing frames 652 arranged in a centrally symmetrical manner, a connecting shaft 654 obliquely passing through the swing seat 651 and connected to the swing frames 652 at both ends, and a driving shaft 653 for driving one of the swing frames 652 to rotate, and the axial joints of the swing frame 652 connected to the driving shaft 653 and the swing frame 652 connected to the loading platform 2 are located on the same horizontal line.

[0040] The application process of the reciprocating rotation of the rotating material rack 2 6 is: the external driving source controls the rotation of the driving shaft 2 653, and the rotation of the driving shaft 2 653 controls the rotation of the swing frame 652 connected to it. The swing frame 652 rotates, and the other swing frame 652 connected to it through the connecting shaft 1 654 rotates accordingly, so that the connecting shaft 1 654 that is inclined and penetrates the swing seat 651 rotates and pushes the swing seat 651 to perform a reciprocating swinging movement at a certain angle. Because the swing seat 651 is coaxially arranged with the transmission gear 2 64, the transmission gear 2 64 also produces a reciprocating swinging state, and through the meshing transmission of the transmission gear 2 64 and the transmission gear ring 2 63, the reciprocating intermittent swing of the ring conveyor belt 2 61 is realized.

[0041] Example 3

[0042] On the basis of Example 1, Figure 3-5 As shown, the gear transmission member includes a transmission gear ring 33 arranged on the inner wall of the circular transmission belt 31, a driving shaft 34 arranged on the worktable 2, a transmission gear 32 axially connected to the worktable 2, and a meshing gear set 35 coaxially arranged with the transmission gear 32 at the driven end and axially connected to the driving shaft 34 at the active end. After the driving structure controls the driving shaft 34 to rotate, the meshing gear set 35 thereon drives the transmission gear 32 to rotate. After the transmission gear 32 rotates, it meshes with the transmission gear ring 33 on the inner side of the circular transmission belt 31, thereby enabling the circular transmission belt 31 to realize rotation transmission relative to the worktable 2, and the workbench 4 is fixed on the circular transmission belt 31 and rotates therewith.

[0043] In order to facilitate the operator to place the culture dish on the carrier 4 and to take pictures and record the culture dish through the camera detection unit 11, the structure of the reciprocating transmission member 62 is further optimized in this embodiment. Figure 3 , 6 As shown, the reciprocating transmission member 62 also includes a reciprocating track 622 arranged on the outer side of the curved end of one side of the ring conveyor belt 61, a transmission rack 9 is provided at the position of the reciprocating track 622 on the loading platform 2, and a swing adjustment member 8 is movably cooperated with the lower end of the position adjustment member 7. The swing adjustment member 8 includes a rotating shaft member 81 arranged at the end of the loading rack 4, a threaded sleeve 83 adjusted at the end of the rotating shaft member 81, and a vertical rod 82 connected to the position adjustment member 7 and arranged at the upper end of the threaded sleeve 83, and the threaded sleeve 83 is transmission-coordinated with the transmission rack 9.

[0044] In specific application, the operator operates through the operating hand hole on the outer side of the microbial incubator 1, and the driving structure 1 drives the rotating material rack 13 to rotate, so that the rotating material rack 13 drives the carrier 14 thereon to rotate at a uniform speed and approach the position of the operator in turn, and stops the driving of the driving structure 1. In the process of the carrier 14 stopping at the position close to the operator, the swing adjustment member 8 on the carrier 14 and the transmission rack 9 produce a transmission match to drive the carrier 25 above the carrier 14 to flip and open. Specifically, firstly, due to the limit of the reciprocating track 2 622, the carrier 25 has the same action process as described in Example 1, that is, the convex rod 74 is pushed by the reciprocating track 2 622 and the position is raised, and the convex rod 74 is driven by the reciprocating track 2 622. The adjusting seat 73, the connecting frame 72, the connecting shaft 71 and the connected material carrier 5 are raised as the position of the protruding rod 74 is raised. Subsequently, when the material carrier 4 rotates to the position of the transmission rack 9 along with the annular conveyor belt 31, the threaded sleeve 83 at the front end of the rotating shaft 81 engages with the transmission rack 9 to transmit, and the rotating shaft 81 swings relative to the material carrier 4. The threaded sleeve 83 drives the connected connecting frame 72 to swing through the vertical rod 82, so that the material carrier 5 connected to the connecting frame 72 through the connecting shaft 71 is also flipped open after swinging. During the flipping process, the protruding rod 74 is restricted by the reciprocating track 622, thereby compressing the elastic reset member 75 and causing displacement relative to the adjusting seat 73.

[0045] After the rack 2 5 is flipped open, the operator places the culture dish body to be cultured on the rack 1 4, and places the culture dish cover body on the rack 2 5 accordingly, and then the culture dish is photographed and recorded by the camera detection unit 11. Multiple culture dishes of the same processing group can be placed on each rack 1 4 as a repeat. After the loading of one rack 1 4 is completed, the rotating rack 1 3 is restarted, and the rack 1 4 is moved. During the movement, the rack 1 4 behind it reaches the loading position, and the previous rack 1 4 is reset because it is not driven by the transmission rack 9 and the reciprocating track 2 622, so that the rack 2 5 is flipped over again to the top of the rack 1 4, and the covering operation of the culture dish is realized.

[0046] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A device for detecting and cultivating bacteriophage viruses in soil, comprising a microbial incubator (1) having a sample carrier (2) therein and a camera detection unit (11) arranged in the microbial incubator (1), characterized in that: The invention also comprises a rotating material rack (3) and a rotating material rack (6) which are arranged concentrically on the loading platform (2), wherein the structure of the rotating material rack (3) comprises an annular transmission belt (31), a gear transmission member (1) which is in transmission cooperation with the annular transmission belt (31), and a driving structure (1) which is used to drive the gear transmission member (1) to drive the annular transmission belt (31) to rotate, and the structure of the rotating material rack (6) comprises an annular transmission belt (61), a gear transmission member (2) which is in transmission cooperation with the annular transmission belt (61), and a driving structure (1) which is used to drive the gear transmission member (1) to drive the annular transmission belt (31) to rotate. A second driving structure for driving the second circular conveyor belt (61) to reciprocate by means of a second wheel transmission member, the second rotating material rack (6) being arranged to reciprocate and having a reciprocating transmission member (62) disposed on its outer side, the reciprocating transmission member (62) comprising a first reciprocating track (621) disposed on the outer side of the straight ends on both sides of the second circular conveyor belt (61) and a second reciprocating track (622) disposed on the outer side of the curved end on one side of the second circular conveyor belt (61), and a transmission rack (9) being disposed on the loading platform (2) at a position corresponding to the second reciprocating track (622); and a plurality of sample racks (4) for carrying culture dish bodies arranged on a rotating material rack (3); a plurality of sample racks (5) for carrying culture dish covers are arranged above the sample racks (4); a position adjusting member (7) is arranged at the end of the sample racks (5); the position adjusting member (7) comprises a connecting shaft (71) arranged at the end of the sample racks (5); a connecting frame (72) arranged at the end of the connecting shaft (71); and an adjusting seat (73) arranged at the end of the connecting frame (72); a telescopic member (76) is arranged at the lower end of the connecting frame (72); and the adjusting seat (73) is provided with a plurality of adjusting members (76) at the lower end of the connecting frame (72). An elastic reset member (75) is provided and a convex rod (74) is slidably connected to the elastic reset member (75). The convex rod (74) is arranged above the reciprocating transmission member (62). The lower end of the position adjustment member (7) is movably matched with a swing adjustment member (8). The swing adjustment member (8) comprises a rotating shaft member (81) provided at the end of the first object carrier (4), a threaded sleeve (83) adjusted at the end of the rotating shaft member (81), and a vertical rod (82) connected to the position adjustment member (7) and arranged at the upper end of the threaded sleeve (83). The threaded sleeve (83) is in transmission cooperation with the transmission rack (9). When the rotating material rack 2 (6) reciprocates, the object rack 2 (5) generates transmission through the position adjustment member (7) and the reciprocating transmission member (62), so that the object rack 2 (5) generates a repeated opening and closing action relative to the object rack 1 (4) to achieve ventilation of the culture dish.

2. The device for detecting and cultivating bacteriophage viruses in soil according to claim 1, characterized in that: The object carrier 1 (4) comprises a limiting seat (41), two connecting seats (42) symmetrically arranged on the limiting seat (41), and an overflow groove (43) opened on the connecting seat (42).

3. The device for detecting and cultivating bacteriophage viruses in soil according to claim 1, characterized in that: The second object carrier (5) is provided with a plurality of negative pressure grippers (10) for gripping the culture dish cover.

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