Application of semen plantaginis extract in preparation of medicine for treating myocardial hypertrophy

Abstract

The invention discloses an application of a semen plantaginis extract in preparation of a medicine for treating myocardial hypertrophy, and relates to the technical field of traditional Chinese medicine. Brown algae active ingredients need to be introduced into the semen plantaginis extract before preparation of the medicine for treating myocardial hypertrophy; the mixing mass ratio of the brown algae active component to the semen plantaginis extract is 10: (1-2); the active polysaccharide in the semen plantaginis extract can promote the repair of myocardial cells by improving the antioxidant capacity and carbonyl removing capacity of an organism. The existing semen plantaginis extract is extracted from dried mature seeds of plantaginis or plantaginis of a plantaginis plant, but the effect of the single semen plantaginis extract is relatively single.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to use of a plantago seed extract in preparing a medicine for treating myocardial hypertrophy. Background Art

[0002] Myocardial hypertrophy is an important pathological manifestation of heart disease, usually caused by long-term cardiovascular pressure load or increased volume load. This pathological change not only affects the contraction and relaxation function of the heart, but may also further develop into serious diseases such as heart failure. Myocardial hypertrophy is a powerful form of compensation, but it is not unlimited. If the cause is long-lasting and cannot be eliminated, the function of the hypertrophic myocardium cannot be maintained normal for a long time and eventually turns to heart failure. Chronic heart failure generally develops gradually on the basis of compensatory myocardial hypertrophy.

[0003] The overall pathological manifestations of myocardial hypertrophy are: increased heart volume, increased weight, concentric hypertrophy of the ventricular wall and decreased ventricular cavity volume; the phenotypic changes of cells are mainly characterized by increased cardiomyocyte volume, increased protein content and proliferation of intercellular matrix.

[0004] Cardiovascular diseases seriously threaten the health of people today, especially their prevalence is also increasing rapidly and tends to occur at a younger age. If pathological stimulation exists for a long time, myocardial hypertrophy will continue to worsen and lead to cardiac decompensation, eccentric hypertrophy, increased number of apoptotic cardiomyocytes and their gradual replacement by fibroblasts, thinning of the ventricular wall, decreased myocardial contractility, and finally severe arrhythmias and heart failure.

[0005] Therefore, it is necessary to treat it in time. Inhibiting the process of myocardial hypertrophy can effectively improve cardiac function and improve the quality of life of patients with cardiovascular disease.

[0006] Based on this, the present invention provides a plantago seed extract for preparing a drug for treating myocardial hypertrophy to solve the existing technical problems. Summary of the invention

[0007] The purpose of the present invention is to provide the use of plantago seed extract in preparing a drug for treating myocardial hypertrophy, so as to solve the deficiencies in the prior art.

[0008] The technical solution adopted by the present invention is as follows:

[0009] Use of a plantago seed extract in preparing a drug for treating myocardial hypertrophy, wherein the plantago seed extract needs to be introduced with brown algae active ingredients before preparing the drug for treating myocardial hypertrophy;

[0010] The mass ratio of the brown algae active ingredient to the plantago seed extract is 10:1-2.

[0011] As a further technical solution:

[0012] The preparation method of the plantago seed extract is as follows:

[0013] S1: Mix wolfberry with water, add 0.1-0.2% pectinase by total weight, beat, let stand for 1 hour, and then inactivate the enzyme to obtain wolfberry slurry;

[0014] S2: crushing the psyllium seeds to obtain psyllium crushed material, mixing the psyllium crushed material with water, adding 0.1-0.2% of cellulase by total weight, and then beating the mixture, standing for 2 hours, and then inactivating the enzyme to obtain psyllium slurry;

[0015] S3: mixing the wolfberry slurry with the psyllium slurry, and then stirring for 30 minutes to obtain a mixed slurry;

[0016] Among them, the stirring speed is 500r / min;

[0017] S4: sterilizing the mixed slurry;

[0018] S5: inoculating the fermentation liquid into the sterilized mixed slurry, stirring and mixing, keeping warm and fermenting for 4-5 hours, and then sterilizing to obtain a mixed fermentation slurry;

[0019] Among them, the stirring speed is 60r / min;

[0020] S6: adding 3 times the mass of ethanol solution to the mixed fermentation slurry, stirring for 4 hours under vacuum conditions, and then filtering to remove the filter residue by high-speed centrifugation to obtain a filtrate, and spray-drying the filtrate to obtain a psyllium extract;

[0021] The mass fraction of the ethanol solution is 75%;

[0022] Among them, the stirring speed is 120r / min.

[0023] As a further technical solution: in S1, wolfberry and water are mixed in a mass ratio of 1:5-6;

[0024] The beating speed is 10000r / min and the beating time is 10min.

[0025] As a further technical solution: the psyllium crushed material in S2 is mixed with water in a mass ratio of 1:3-4;

[0026] The beating speed is 8000r / min and the beating time is 15min.

[0027] As a further technical solution: the mass fraction of the psyllium slurry in the mixed slurry in S3 is 75-90%.

[0028] As a further technical solution: the sterilization in S4 adopts 121°C steam sterilization treatment, wherein the sterilization time adopted is 10 minutes.

[0029] As a further technical solution: based on the total mass of the mixed slurry, the inoculation amount of the fermentation liquid in S5 is 3-3.5% of the mass of the mixed slurry;

[0030] The fermented bacterial liquid is Lactobacillus rhamnosus liquid;

[0031] Among them, the concentration of Lactobacillus rhamnosus liquid was 1.33×10 5 cfu / mL.

[0032] As a further technical solution: the preparation method of the brown algae active ingredient is:

[0033] Firstly, the brown algae is preliminarily crushed to obtain a preliminarily crushed brown algae material;

[0034] Among them, the average particle size of the preliminary crushed brown algae is 50 mesh;

[0035] Add water to the preliminary crushed brown algae, adjust the temperature to 60°C, and keep the temperature in a water bath for 30 minutes to obtain a brown algae slurry;

[0036] Steam explosion treatment of brown algae slurry:

[0037] Specifically, the brown algae slurry is subjected to steam explosion treatment, wherein the air pressure of the steam explosion is 1.2-1.5 MPa, the temperature of the steam explosion is 118-122° C., and the steam release time is 0.8 seconds;

[0038] When steam explosion is performed, water vapor is first introduced into the brown algae slurry. When the steam pressure reaches 1.0 MPa, the steam is stopped and compressed nitrogen is introduced to maintain the pressure at 1.2-1.5 MPa, and the steam is released and exploded.

[0039] To the brown algae slurry after steam explosion treatment, water in an amount twice its mass was added, and water extraction treatment was performed at a speed of 500 r / min for 3 hours, and then high-speed centrifugation was performed for 5 minutes. The slurry was filtered to remove the filter residue, and the supernatant was taken and spray-dried to obtain the brown algae active ingredients.

[0040] As a further technical solution: the brown algae is Sargassum;

[0041] Among them, the mass ratio of the Sargassum crushed material and water is 1:7-8.

[0042] As a further technical solution, the rotation speed of the high-speed centrifuge is 11000r / min.

[0043] The pharmaceutical dosage forms of the drug for treating myocardial hypertrophy prepared by mixing the brown algae active ingredients with the plantago seed extract of the present invention include but are not limited to tablets, capsules, oral liquids, granules, ointments, powders, etc. The drug for treating myocardial hypertrophy prepared by mixing the brown algae active ingredients with the plantago seed extract is all taken orally.

[0044] When the brown algae active ingredients and the psyllium extract of the present invention are taken orally, combined with measures including lifestyle adjustment, exercise rehabilitation, etc., it can further help patients improve their cardiovascular health and enhance their quality of life.

[0045] Myocardial hypertrophy not only affects the normal function of the heart, but may also lead to serious consequences such as heart failure. Therefore, timely improvement of the symptoms of myocardial hypertrophy is of great positive significance for improving the quality of life of patients.

[0046] Beneficial effects:

[0047] The active polysaccharide in the plantago seed extract of the present invention can promote the repair of myocardial cells by improving the body's antioxidant capacity and carbonyl scavenging capacity.

[0048] The existing psyllium seed extract is extracted from the dried mature seeds of Plantago or Plantago asiatica of the Plantaginaceae family. However, the efficacy of a single psyllium seed extract is relatively single.

[0049] Therefore, the present invention introduces wolfberry into the preparation process of the plantago seed extract, uses wolfberry slurry and plantago seed as culture medium, performs specific fermentation treatment, and then extracts to obtain a special composite plantago seed extract. It has been verified through experiments that the special composite plantago seed extract can significantly improve the cardiac function of mice with induced myocardial hypertrophy, and in combination with the active ingredients of brown algae, can inhibit the enlargement of the myocardial morphology, thereby providing a strong basis for its application in the treatment of myocardial hypertrophy.

[0050] In addition, the extract of plantain seeds can improve cardiac microcirculation, increase myocardial blood supply, and help relieve myocardial ischemia and hypoxia, which is of great significance for improving the cardiac function of patients with myocardial hypertrophy. At the same time, the diuretic effect of plantain seeds extract can also help alleviate edema symptoms, further improve the quality of life of patients with myocardial hypertrophy, promote recovery, and shorten the recovery period.

[0051] The present invention can promote the repair of myocardial hypertrophy by combining the brown algae active ingredients with the plantago seed extract. The brown algae active ingredients and the plantago seed extract in the present invention can play the following roles:

[0052] 1. Regulation of signal transduction pathways: The occurrence of myocardial hypertrophy is closely related to the abnormal activation of multiple signal transduction pathways. For example, calcium ion signaling pathway, MAPK signaling pathway and PI 3K / Akt signaling pathway are all involved in the regulation of myocardial hypertrophy. The present invention, through the combination of the two, can intervene in the activity of these signaling pathways, thereby effectively inhibiting the development of myocardial hypertrophy and promoting its repair, and promoting the normalization of myocardial cell function.

[0053] 2. Regulation of gene expression: The occurrence of myocardial hypertrophy is also accompanied by a series of changes in gene expression. The brown algae active ingredients prepared in the present invention and some components in the plantago extract will act as signal factors, which can induce the activation of genes that promote myocardial growth, thereby playing a more significant role in regulating myocardial cells.

[0054] 3. Balance between cell apoptosis and regeneration: During myocardial hypertrophy, some myocardial cells may undergo apoptosis due to excessive stress. However, there is also a regeneration process of myocardial cells. Therefore, the brown algae active ingredients in the present invention can promote the regeneration of myocardial cells and reduce apoptosis during this process, thereby helping to restore the normal structure and function of the heart.

[0055] The brown algae active ingredient used in the present invention is obtained by a series of steps of preparation from Sargassum, and its unique growth environment and biological characteristics endow it with rich nutrients and active substances. Firstly, from the perspective of chemical composition, Sargassum contains a large amount of seaweed polysaccharides, and these polysaccharides have unique biological activities and functions, such as enhancing immunity, anti-tumor, and anti-oxidation. In addition, Sargassum also contains some special alkaloids and phenolic compounds, and these compounds have antibacterial and anti-inflammatory effects. The present invention has a strong effect on the treatment of cardiomyopathy by combining the brown algae active ingredient with the Plantago seed extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 The present invention is a flow chart of the preparation method of Plantago asiatica extract;

[0057] Figure 2 The figure is a bar graph of cardiac output of each experimental group. DETAILED DESCRIPTION

[0058] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] Use of a plantago seed extract in preparing a drug for treating myocardial hypertrophy, wherein the plantago seed extract needs to be introduced with brown algae active ingredients before preparing the drug for treating myocardial hypertrophy;

[0060] The mass ratio of the brown algae active ingredient to the plantago seed extract is 10:1-2.

[0061] The chemical components of Plantago seed extract include phenylethanol glycosides, iridoids, flavonoids, alkaloids and polysaccharides, carotenoids, organic acids and phenolic chemical components.

[0062] The preparation method of the plantago seed extract is as follows:

[0063] S1: Mix wolfberry with water, add 0.1-0.2% pectinase by total weight, beat, let stand for 1 hour, and then inactivate the enzyme to obtain wolfberry slurry;

[0064] S2: crushing the psyllium seeds to obtain psyllium crushed material, mixing the psyllium crushed material with water, adding 0.1-0.2% of cellulase by total weight, and then beating the mixture, standing for 2 hours, and then inactivating the enzyme to obtain psyllium slurry;

[0065] S3: mixing the wolfberry slurry with the psyllium slurry, and then stirring for 30 minutes to obtain a mixed slurry;

[0066] Among them, the stirring speed is 500r / min;

[0067] S4: sterilizing the mixed slurry;

[0068] S5: inoculating the fermentation liquid into the sterilized mixed slurry, stirring and mixing, keeping warm and fermenting for 4-5 hours, and then sterilizing to obtain a mixed fermentation slurry;

[0069] Among them, the stirring speed is 60r / min;

[0070] S6: adding 3 times the mass of ethanol solution to the mixed fermentation slurry, stirring for 4 hours under vacuum conditions, and then filtering to remove the filter residue by high-speed centrifugation to obtain a filtrate, and spray-drying the filtrate to obtain a psyllium extract;

[0071] The mass fraction of the ethanol solution is 75%;

[0072] Among them, the stirring speed is 120r / min.

[0073] In S1, wolfberry and water are mixed in a mass ratio of 1:5-6;

[0074] The beating speed is 10000r / min and the beating time is 10min.

[0075] The psyllium seeds crushed material in S2 is mixed with water in a mass ratio of 1:3-4;

[0076] The beating speed is 8000r / min and the beating time is 15min.

[0077] The mass fraction of the psyllium slurry in the mixed slurry in S3 is 75-90%.

[0078] In S4, the sterilization is carried out by steam sterilization at 121°C, and the sterilization time is 10 minutes.

[0079] Based on the total mass of the mixed slurry, the inoculation amount of the fermentation liquid in S5 is 3-3.5% of the mass of the mixed slurry;

[0080] The fermented bacterial liquid is Lactobacillus rhamnosus liquid;

[0081] Among them, the concentration of Lactobacillus rhamnosus liquid was 1.33×10 5 cfu / mL.

[0082] The preparation method of the brown algae active ingredient is as follows:

[0083] Firstly, the brown algae is preliminarily crushed to obtain a preliminarily crushed brown algae material;

[0084] Among them, the average particle size of the preliminary crushed brown algae is 50 mesh;

[0085] Add water to the preliminary crushed brown algae, adjust the temperature to 60°C, and keep the temperature in a water bath for 30 minutes to obtain a brown algae slurry;

[0086] Steam explosion treatment of brown algae slurry:

[0087] Specifically, the brown algae slurry is subjected to steam explosion treatment, wherein the air pressure of the steam explosion is 1.2-1.5 MPa, the temperature of the steam explosion is 118-122° C., and the steam release time is 0.8 seconds;

[0088] When steam explosion is performed, water vapor is first introduced into the brown algae slurry. When the steam pressure reaches 1.0 MPa, the steam is stopped and compressed nitrogen is introduced to maintain the pressure at 1.2-1.5 MPa, and the steam is released and exploded.

[0089] To the brown algae slurry after steam explosion treatment, water in an amount twice its mass was added, and water extraction treatment was performed at a speed of 500 r / min for 3 hours, and then high-speed centrifugation was performed for 5 minutes. The slurry was filtered to remove the filter residue, and the supernatant was taken and spray-dried to obtain the brown algae active ingredients.

[0090] The brown algae is Sargassum;

[0091] Among them, the mass ratio of the Sargassum crushed material and water is 1:7-8.

[0092] The rotation speed of the high-speed centrifuge is 11000 r / min.

[0093] The following are specific embodiments:

[0094] Example 1

[0095] The mass ratio of the brown algae active ingredients to the plantago seed extract is 10:1.

[0096] The preparation method of the plantago seed extract is as follows:

[0097] S1: Mix wolfberry with water, add 0.1% pectinase by total weight, beat, let stand for 1 hour, and then inactivate the enzyme to obtain wolfberry slurry;

[0098] Wolfberry and water are mixed in a mass ratio of 1:5;

[0099] The beating speed is 10000r / min and the beating time is 10min.

[0100] S2: crushing the psyllium seeds to obtain psyllium crushed material, mixing the psyllium crushed material with water, adding 0.1% of the total weight of cellulase, and then beating, standing for 2 hours, and then inactivating the enzyme to obtain psyllium slurry; the psyllium crushed material and water are mixed in a mass ratio of 1:3;

[0101] The beating speed is 8000r / min and the beating time is 15min.

[0102] S3: Mix the wolfberry slurry and the psyllium slurry, and then stir for 30 minutes to obtain a mixed slurry; the mass fraction of the psyllium slurry in the mixed slurry is 75%.

[0103] Among them, the stirring speed is 500r / min;

[0104] S4: sterilize the mixed slurry; the sterilization is carried out by using 121° C. steam sterilization, wherein the sterilization time is 10 minutes.

[0105] S5: inoculating the fermentation liquid into the sterilized mixed slurry, stirring and mixing, keeping warm and fermenting for 4-5 hours, and then sterilizing to obtain a mixed fermentation slurry;

[0106] The stirring speed is 60r / min; based on the total mass of the mixed slurry, the inoculation amount of the fermentation liquid in S5 is 3% of the mass of the mixed slurry;

[0107] The fermented bacterial liquid is Lactobacillus rhamnosus liquid;

[0108] Among them, the concentration of Lactobacillus rhamnosus liquid was 1.33×10 5 cfu / mL.

[0109] S6: adding ethanol solution in an amount three times the mass of the mixed fermentation slurry, stirring for 4 hours under vacuum conditions, and then filtering to remove the residue by high-speed centrifugation to obtain a filtrate, and spray-drying the filtrate to obtain a Plantago seed extract;

[0110] The mass fraction of the ethanol solution is 75%;

[0111] Among them, the stirring speed is 120r / min.

[0112] The preparation method of brown algae active ingredients is as follows:

[0113] Firstly, the brown algae is preliminarily crushed to obtain a preliminarily crushed brown algae material;

[0114] Among them, the average particle size of the preliminary crushed brown algae is 50 mesh;

[0115] Add water to the preliminary crushed brown algae, adjust the temperature to 60°C, and keep the temperature in a water bath for 30 minutes to obtain a brown algae slurry;

[0116] Steam explosion treatment of brown algae slurry:

[0117] Specifically, the brown algae slurry is subjected to steam explosion treatment, wherein the air pressure of the steam explosion is 1.2 MPa, the temperature of the steam explosion is 118° C., and the steam release time is 0.8 seconds;

[0118] When steam explosion is performed, water vapor is first introduced into the brown algae slurry. When the steam pressure reaches 1.0 MPa, the steam is stopped and compressed nitrogen is introduced to maintain the pressure at 1.2 MPa, and the steam is released and exploded.

[0119] To the brown algae slurry after steam explosion treatment, water in an amount twice its mass was added, and water extraction treatment was performed at a speed of 500 r / min for 3 hours, and then high-speed centrifugation was performed for 5 minutes. The slurry was filtered to remove the filter residue, and the supernatant was taken and spray-dried to obtain the brown algae active ingredients.

[0120] The brown algae is Sargassum;

[0121] Among them, the mass ratio of Sargassum crushed material and water is 1:7.

[0122] The rotation speed of the high-speed centrifuge is 11000 r / min.

[0123] Example 2

[0124] The difference from Example 1 is that the mass ratio of the brown algae active ingredient to the plantago seed extract is 10:1.5.

[0125] Example 3

[0126] The difference from Example 1 is that the mass ratio of the brown algae active ingredient to the plantago seed extract is 10:2.

[0127] Example 4

[0128] The difference from Example 1 is that the mass fraction of the psyllium slurry in the mixed slurry is 80%.

[0129] Example 5

[0130] The difference from Example 1 is that the mass fraction of the psyllium slurry in the mixed slurry is 85%.

[0131] Example 6

[0132] The difference from Example 1 is that the mass fraction of the psyllium slurry in the mixed slurry is 90%.

[0133] Comparative Example 1:

[0134] On the basis of Example 2, no brown algae active ingredient is added, and the rest of the technical scheme is the same as Example 2.

[0135] Comparative Example 2:

[0136] Based on Example 2, no fermentation treatment was performed during the preparation of the Plantago asiatica extract;

[0137] The preparation method of the plantago seed extract is as follows:

[0138] S1: Mix wolfberry with water, add 0.1% pectinase by total weight, beat, let stand for 1 hour, and then inactivate the enzyme to obtain wolfberry slurry;

[0139] Wolfberry and water are mixed in a mass ratio of 1:5;

[0140] The beating speed is 10000r / min and the beating time is 10min.

[0141] S2: crushing the psyllium seeds to obtain psyllium crushed material, mixing the psyllium crushed material with water, adding 0.1% of the total weight of cellulase, and then beating, standing for 2 hours, and then inactivating the enzyme to obtain psyllium slurry; the psyllium crushed material and water are mixed in a mass ratio of 1:3;

[0142] The beating speed is 8000r / min and the beating time is 15min.

[0143] S3: Mix the wolfberry slurry and the psyllium slurry, and then stir for 30 minutes to obtain a mixed slurry; the mass fraction of the psyllium slurry in the mixed slurry is 75%.

[0144] Among them, the stirring speed is 500r / min;

[0145] S4: adding ethanol solution to the mixed slurry, stirring for 4 hours under vacuum conditions, and then filtering to remove the filter residue through high-speed centrifugation to obtain a filtrate, and spray-drying the filtrate to obtain a Plantago seed extract;

[0146] The mass fraction of the ethanol solution is 75%;

[0147] Among them, the stirring speed is 120r / min.

[0148] test

[0149] 100 SPF Kunming male mice, weighing 20 g ± 1 g, 7 weeks old;

[0150] After 100 mice had eaten normally for one week, they were randomly divided into 10 groups, with 10 mice in each group. Isoproterenol was dissolved in normal saline and injected subcutaneously twice a day at 20 mg / kg / day for 2 weeks to induce myocardial hypertrophy in mice in 9 groups as the model group. One group of normal mice was left as the blank control group.

[0151] Animal dosing and body weight measurement

[0152] The examples and comparative examples were used for administration (dosage of 20 mg / kg), respectively, added to pure water, with a mass concentration of 30%, and 8 groups in the model group were given oral administration for 5 consecutive days, once a day, and one model group was given an equal amount of pure water;

[0153] Cardiac ultrasound was performed on each group of mice after the above experimental treatment:

[0154] After the mice were anesthetized by inhalation of 1.2% isoflurane, the apex of the heart and the 3rd and 4th intercostal spaces on the left side of the bone were explored by Visual Sonics Vevo2100 Doppler echocardiograph, 30MHz probe, M-Mode, and the ejection fraction (EF, %), stroke volume (SV), cardiac output (CO), and fractional shortening (FS, %) were recorded, and the average values ​​were taken.

[0155] Table 1

[0156]

[0157]

[0158] It can be seen from Table 1 that the brown algae active ingredients mixed with the plantago seed extract can improve the problem of impaired myocardial function caused by myocardial hypertrophy.

[0159] The hearts of mice were taken and the sizes of cardiomyocytes of mice in each group were detected. The cross-sectional area of ​​cardiomyocytes was determined using the automatic image analysis system of Image Pro Plus 6.0:

[0160] Table 2

[0161]

[0162]

[0163] It can be seen from Table 2 that the active ingredients of brown algae mixed with the plantago extract can have a therapeutic effect on myocardial hypertrophy.

[0164] The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the scope of implementation shown. Any changes made in accordance with the concept of the present invention, or modifications to equivalent embodiments with equivalent changes, which still do not exceed the spirit covered by the specification, should be within the protection scope of the present invention.

Claims

1. Use of Plantago seed extract in preparing a drug for treating myocardial hypertrophy, characterized in that: The plantago seed extract needs to be introduced into brown algae active ingredients before preparing the drug for treating myocardial hypertrophy; The mass ratio of the brown algae active ingredient to the plantago seed extract is 10:1-2.

2. Use of the Plantago seed extract according to claim 1 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: The preparation method of the plantago seed extract is as follows: S1: Mix wolfberry with water, add 0.1-0.2% pectinase by total weight, beat, let stand for 1 hour, and then inactivate the enzyme to obtain wolfberry slurry; S2: crushing the psyllium seeds to obtain psyllium crushed material, mixing the psyllium crushed material with water, adding 0.1-0.2% of cellulase by total weight, and then beating the mixture, standing for 2 hours, and then inactivating the enzyme to obtain psyllium slurry; S3: mixing the wolfberry slurry with the psyllium slurry, and then stirring for 30 minutes to obtain a mixed slurry; Among them, the stirring speed is 500r / min; S4: sterilizing the mixed slurry; S5: inoculating the fermentation liquid into the sterilized mixed slurry, stirring and mixing, keeping warm and fermenting for 4-5 hours, and then sterilizing to obtain a mixed fermentation slurry; Among them, the stirring speed is 60r / min; S6: adding 3 times the mass of ethanol solution to the mixed fermentation slurry, stirring for 4 hours under vacuum conditions, and then filtering to remove the filter residue by high-speed centrifugation to obtain a filtrate, and spray-drying the filtrate to obtain a psyllium extract; The mass fraction of the ethanol solution is 75%; Among them, the stirring speed is 120r / min.

3. Use of the Plantago seed extract according to claim 2 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: In S1, wolfberry and water are mixed in a mass ratio of 1:5-6; The beating speed is 10000r / min and the beating time is 10min.

4. Use of the Plantago seed extract according to claim 2 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: The psyllium seeds crushed material in S2 is mixed with water in a mass ratio of 1:3-4; The beating speed is 8000r / min and the beating time is 15min.

5. Use of the Plantago seed extract according to claim 2 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: The mass fraction of the psyllium slurry in the mixed slurry in S3 is 75-90%.

6. Use of the Plantago seed extract according to claim 2 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: In S4, sterilization is performed by steam sterilization at 121°C, wherein the sterilization time is 10 minutes.

7. Use of the Plantago seed extract according to claim 2 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: Based on the total mass of the mixed slurry, the inoculation amount of the fermentation liquid in S5 is 3-3.5% of the mass of the mixed slurry; The fermented bacterial liquid is Lactobacillus rhamnosus liquid; Among them, the concentration of Lactobacillus rhamnosus liquid was 1.33×10 5 cfu / mL.

8. Use of the Plantago seed extract according to claim 1 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: The preparation method of the brown algae active ingredient is as follows: Firstly, the brown algae is preliminarily crushed to obtain a preliminarily crushed brown algae material; Among them, the average particle size of the preliminary crushed brown algae is 50 mesh; Add water to the preliminary crushed brown algae, adjust the temperature to 60°C, and keep the temperature in a water bath for 30 minutes to obtain a brown algae slurry; Steam explosion treatment of brown algae slurry: Specifically, the brown algae slurry is subjected to steam explosion treatment, wherein the air pressure of the steam explosion is 1.2-1.5 MPa, the temperature of the steam explosion is 118-122° C., and the steam release time is 0.8 seconds; When steam explosion is performed, water vapor is first introduced into the brown algae slurry. When the steam pressure reaches 1.0 MPa, the steam is stopped and compressed nitrogen is introduced to maintain the pressure at 1.2-1.5 MPa, and the steam is released and exploded. To the brown algae slurry after steam explosion treatment, water in an amount twice its mass was added, and water extraction treatment was performed at a speed of 500 r / min for 3 hours, and then high-speed centrifugation was performed for 5 minutes. The slurry was filtered to remove the filter residue, and the supernatant was taken and spray-dried to obtain the brown algae active ingredients.

9. Use of the Plantago seed extract according to claim 8 in the preparation of a drug for treating myocardial hypertrophy, characterized in that: The brown algae is Sargassum; Among them, the mass ratio of the Sargassum crushed material and water is 1:7-8.

10. Use of the Plantago seed extract according to claim 8 in preparing a drug for treating myocardial hypertrophy, characterized in that: The rotation speed of the high-speed centrifuge is 11000 r / min.

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