Composite agricultural bacterial fertilizer and application thereof

Abstract

The invention discloses a compound agricultural bacterial fertilizer and application thereof, and belongs to the technical field of fertilizers. The compound agricultural bacterial fertilizer comprises the following effective components: bacillus subtilis, bacillus licheniformis and bacillus velezensis, and further comprises brassinolide and sophorolipid. After the compound agricultural bacterial fertilizer is applied, the yield and quality of crops are improved, the yield improvement effect is 8.22-8.83% or 8.44-9.28%, the soluble solid content and the soluble total sugar content of the crops are improved, and the titratable acidity is reduced. The compound agricultural bacterial fertilizer with a good effect is provided for promoting crop growth and improving the crop yield and quality.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers and relates to a composite agricultural bacterial fertilizer and application thereof. Background Art

[0002] Bacillus subtilis is a type of Bacillus. During its growth, it produces active substances such as subtilisin, polymyxin, nystatin, and gramicidin, which have a significant inhibitory effect on pathogenic bacteria. The bacteria itself synthesizes enzymes such as α-amylase, protease, lipase, cellulase, and can synthesize vitamin B1, B2, B6, niacin and other B vitamins, which promote the rooting and root growth of crops, and make the stems of crops thick and the seedlings strong. Bacillus subtilis also has the function of improving soil, which can regulate the nutrient content in the soil, change the structure of microbial flora in the soil, and decompose residual pesticides in the soil.

[0003] Bacillus licheniformis is a Gram-positive thermophilic bacterium commonly found in soil. It can adjust the dysbiosis to achieve the purpose of treatment, promote the growth of normal physiological anaerobic bacteria in the intestine, and restore intestinal function; it can induce the body to produce antibacterial active substances and kill pathogenic bacteria. It also has a unique biological oxygen-depriving mechanism, forming an anaerobic environment and inhibiting the growth and reproduction of pathogenic bacteria.

[0004] Bacillus velezensis is a Gram-positive bacterium that can secrete and produce a variety of bioactive substances, including enzymes, antimicrobial proteins, lipopeptide antibiotics, polyketide antibiotics, plant hormones, etc. Bacillus velezensis can effectively inhibit a variety of plant pathogens, such as Phytophthora capsici, Fusarium solani, Fusarium oxysporum, Rhizoctonia solani, Brown spot fungus, Stem spot fungus, Botrytis cinerea var. dianthus and Sclerotinia sclerotiorum of rapeseed, and effectively prevent and control a variety of plant diseases.

[0005] The existing technology mostly uses chemical fertilizers and chemical pesticides to provide nutrients for crops, reduce the incidence of diseases, and indirectly increase crop yields. However, with the long-term application of chemical fertilizers and chemical pesticides, the nutrients in the soil are unbalanced, the microbial flora structure is disordered, and pests and diseases are aggravated, which ultimately leads to a decrease in crop yields. Therefore, it is of great significance to provide a composite agricultural bacterial fertilizer with microorganisms as the main active ingredient to improve crop yield and quality. Summary of the invention

[0006] In order to develop a composite agricultural bacterial fertilizer with microorganisms as the main effective ingredients to improve crop yield and quality, the present invention provides a composite agricultural bacterial fertilizer and its application. The effective ingredients of the composite agricultural bacterial fertilizer include: Bacillus subtilis, Bacillus licheniformis and Bacillus velez, and also include brassinolide and sophorolipids. The composite agricultural bacterial fertilizer can promote the growth of crops, improve crop yield and quality, and promote fruit coloring.

[0007] Specifically, the present invention provides a composite agricultural bacterial fertilizer, wherein the effective components of the composite agricultural bacterial fertilizer include: Bacillus subtilis, Bacillus licheniformis and Bacillus velez, and the effective components of the composite agricultural bacterial fertilizer also include brassinolide and sophorolipids, and the effective viable bacteria count of the composite agricultural bacterial fertilizer is ≥2.0×10 10 CFU / g. The mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velez is 12-25:2-5:3-8; preferably, the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velez is 16:4:6. In terms of mass percentage, the amount of brassinolide added is 0.03%, and the amount of sophorolipid added is 0.2%. The composite agricultural bacterial fertilizer provided by the present invention can improve the yield and quality of crops, and can also promote fruit coloring.

[0008] Furthermore, the present invention has learned through field experiments that the composite agricultural bacterial fertilizer has a promoting effect on the yield and quality of ruby ​​plums. After applying the composite agricultural bacterial fertilizer, the yield of ruby ​​plums increased to 366.43-368.51 kg / 90m 2 Compared with the control group, the yield increased by 8.22-8.83%; the edible rate increased from 96.48% to 97.42-97.46%; the soluble solids content increased from 15.7% to 17.1-17.4%; the soluble total sugar content increased from 12.31% to 13.33-13.48%; the titratable acidity decreased from 0.79% to 0.61-0.64%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 16:4:6, the effect of improving the yield and quality of ruby ​​plum was optimal.

[0009] Furthermore, the present invention has learned through field experiments that the composite agricultural bacterial fertilizer has a promoting effect on the yield, quality and coloring rate of Fuji apples. After applying the composite agricultural bacterial fertilizer, the yield of Fuji apples increased to 688.02-693.37 kg / 120 m 2Compared with the control group, the yield increased by 8.44-9.28%; the soluble solid content increased from 13.3% to 14.3-14.5%; the soluble total sugar content increased from 11.17% to 12.25-12.34%; the titratable acidity decreased from 0.33% to 0.20-0.21%; the coloring rate increased from 70.23% to 80.45-84.06%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 16:4:6, the improvement effect of Fuji apple yield, quality and coloring was optimal.

[0010] The present invention seeks to protect the use of the above-mentioned composite agricultural bacterial fertilizer in increasing the yield of crops, which include plums and apples.

[0011] The present invention seeks to protect the use of the above-mentioned composite agricultural bacterial fertilizer in improving the quality of crops. Improving the quality of crops specifically includes increasing the edible rate of crops, increasing the soluble solids content, increasing the soluble total sugar content and reducing the titratable acidity.

[0012] In addition, the present invention seeks to protect the use of the above-mentioned composite agricultural bacterial fertilizer in improving fruit coloring.

[0013] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0014] The effective components of the composite agricultural bacterial fertilizer provided by the present invention include Bacillus subtilis, Bacillus licheniformis and Bacillus velez, and also include brassinolide and sophorolipid, the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velez is 12-25:2-5:3-8, and in terms of mass percentage, the addition amount of brassinolide is 0.03%, and the addition amount of sophorolipid is 0.2%. The composite agricultural bacterial fertilizer of the present invention can improve the yield and quality of crops, and can also promote fruit coloring.

[0015] The composite agricultural bacterial fertilizer of the present invention has a promoting effect on the yield and quality of ruby ​​plums. After applying the composite agricultural bacterial fertilizer, the yield of ruby ​​plums is increased to 366.43-368.51 kg / 90m 2, compared with the control group, the yield increased by 8.22-8.83%; the edible rate increased from 96.48% to 97.42-97.46%; the soluble solid content increased from 15.7% to 17.1-17.4%; the soluble total sugar content increased from 12.31% to 13.33-13.48%; the titratable acidity decreased from 0.79% to 0.61-0.64%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velezii was 16:4:6, the yield and quality of ruby ​​plum were the best. It can be seen that the yield and quality of ruby ​​plums were improved after applying the composite agricultural fertilizer provided by the present invention. After exceeding the ratio of the composite agricultural fertilizer provided by the present invention, the effect of improving the yield and quality of ruby ​​plums is poor; the composite agricultural fertilizer provided by the present invention is also added with brassinolide and sophorolipids, which can further improve the effect of the fertilizer.

[0016] The composite agricultural bacterial fertilizer of the present invention has a promoting effect on the yield, quality and coloring rate of Fuji apples. After applying the composite agricultural bacterial fertilizer, the yield of Fuji apples is increased to 688.02-693.37 kg / 120 m 2 , compared with the control group, the yield increased by 8.44-9.28%; the soluble solid content increased from 13.3% to 14.3-14.5%; the soluble total sugar content increased from 11.17% to 12.25-12.34%; the titratable acidity decreased from 0.33% to 0.20-0.21%; the coloring rate increased from 70.23% to 80.45-84.06%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velezii was 16:4:6, the yield, quality and coloring of Fuji apples were the best. It can be seen that the yield, quality and coloring rate of Fuji apples were improved after applying the composite agricultural fertilizer provided by the present invention. After exceeding the ratio of the composite agricultural fertilizer provided by the present invention, the improvement effect on the yield, quality and coloring rate of Fuji apples is poor; the composite agricultural fertilizer provided by the present invention is also added with brassinolide and sophorolipids, which can further improve the effect of the fertilizer. DETAILED DESCRIPTION

[0017] The technical scheme of the present invention is now described in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in the following embodiments are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0018] Bacillus subtilis was purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd., with an effective viable count of 100 billion CFU / g;

[0019] Bacillus licheniformis was purchased from Guangzhou Zhenwei Microbiology Technology Co., Ltd., with an effective viable count of 100 billion CFU / g;

[0020] Bacillus Velezii was purchased from Shandong Yiwu Biotechnology Co., Ltd., with an effective viable count of 100 billion CFU / g;

[0021] Brassinolide was purchased from Wuhan Xinweiye Chemical Co., Ltd. with a purity of 90%.

[0022] Sophorolipids were purchased from Hubei Wei's Chemical Reagent Co., Ltd. with a purity of 50%.

[0023] Example 1

[0024] This embodiment provides a formulation of a composite agricultural bacterial fertilizer and the bacterial content of the composite agricultural bacterial fertilizer.

[0025] 1. Wettable powder

[0026] The compound agricultural fertilizer includes 25kg of Bacillus subtilis, 2kg of Bacillus licheniformis, 3kg of Bacillus velezii, 0.033kg of brassinolide, 0.4kg of sophorolipid, 5kg of lignin sulfonate, 5kg of sodium dodecyl sulfonate, 1kg of magnesium aluminum silicate, and kaolin is supplemented to 100kg.

[0027] Weigh each component according to the above ratio, add the materials into a blender and mix them evenly, grind them with a jet mill, and pass them through a 600-mesh standard sieve to obtain a wettable powder of the composite agricultural bacterial fertilizer.

[0028] 2. Water dispersible granules

[0029] The agricultural composition of the composite agricultural bacterial fertilizer includes 12kg of Bacillus subtilis, 5kg of Bacillus licheniformis, 8kg of Bacillus velezii, 0.033kg of brassinolide, 0.4kg of sophorolipid, 3kg of polycarboxylate, 4kg of sodium lauryl sulfate, 4kg of sodium sulfate, 5kg of polyvinyl alcohol, and kaolin is supplemented to 100kg.

[0030] Weigh each component according to the above mass proportion, add the material into a blender and mix evenly, then grind it into powder by a jet mill, pass it through a 600-mesh standard sieve, add it into a kneader and knead it into a plastic material, finally put the material into an extrusion granulator for extrusion granulation, and after granulation, dry and sieve to obtain water-dispersible granules of the composite agricultural bacterial fertilizer.

[0031] 3. Bacteria content of compound agricultural fertilizer

[0032] The viable bacteria content of the prepared composite agricultural fertilizer was determined, and the effective viable bacteria count of each batch of composite agricultural fertilizer with different mass ratios was ≥2.0×10 10 CFU / g. According to the national standard NY 227-1994 for microbial fertilizers, the total number of viable bacteria in solid microbial fertilizers should be ≥2.0×10 8CFU / g, it can be seen that the total number of viable bacteria in the composite agricultural fertilizer prepared by the present invention reaches the national standard for the number of viable bacteria of solid microbial fertilizer.

[0033] Example 2

[0034] This embodiment provides the application of composite agricultural bacterial fertilizer in the cultivation of ruby ​​plums.

[0035] The test was conducted in the plum garden of Liulin Village, Sanmenxia City, Henan Province. The test variety was Ruby Plum, which was 6 years old and planted at a spacing of 3m×2.5m. The formulation of the agricultural composite bacterial fertilizer used in the test was a wettable powder, and the preparation method was the same as in Example 1.

[0036] The experiment involved a total of 12 treatments, namely, experiment 1: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 12:5:8; experiment 2: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 16:4:6; experiment 3: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 20:3:4; experiment 4: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 25:2:3; experiment 5: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 28:1:2; experiment 6: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as experiment 1, The Bacillus Velez was replaced by Bacillus megaterium in equal amounts; Experiment 7: Bacillus subtilis was not added; Experiment 8: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as in Experiment 1, and the sophorolipid was replaced by lecithin in equal amounts; Experiment 9: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as in Experiment 1, and the sophorolipid was replaced by lauric acid in equal amounts; Experiment 10: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as in Experiment 1, and sophorolipid was not added; Experiment 11: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as in Experiment 1, and brassinolide was not added; Experiment 12: control group, no agricultural compound bacterial fertilizer was applied.

[0037] The randomized block arrangement was used with three replicates, and each plot was 90 m 2 The compound agricultural fertilizer was diluted 600 times and irrigated the roots of the Ruby Plum plants on April 10, April 25 and May 10, with 3000 mL per plant. At the same time, 0.2 wt% potassium dihydrogen phosphate solution was sprayed on the leaves at 70 mL / 90 m 2 , and the management after application was the same as that of the field. At the fruit maturity stage, 100 fruit samples with the same maturity were collected from different positions of 15 fruit trees in each treatment, and the fruit yield, edible rate, soluble solid content, soluble total sugar content and titratable acid were statistically analyzed. The statistical results are shown in Table 1.

[0038] Table 1: Effects of compound agricultural bacterial fertilizer on yield and quality of Ruby plum

[0039]

[0040] As shown in Table 1, the composite agricultural bacterial fertilizer provided by the present invention has a promoting effect on the yield and quality of ruby ​​plum. After applying the composite agricultural bacterial fertilizer, the yield of ruby ​​plum increased to 366.43-368.51 kg / 90m 2 , compared with the control group, the yield increased by 8.22-8.83%; the edible rate increased from 96.48% to 97.42-97.46%; the soluble solid content increased from 15.7% to 17.1-17.4%; the soluble total sugar content increased from 12.31% to 13.33-13.48%; the titratable acidity decreased from 0.79% to 0.61-0.64%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velezii was 16:4:6, the yield and quality of ruby ​​plum were the best. It can be seen that the yield and quality of ruby ​​plums were improved after applying the composite agricultural fertilizer provided by the present invention. After exceeding the ratio of the composite agricultural fertilizer provided by the present invention, the effect of improving the yield and quality of ruby ​​plums is poor; the composite agricultural fertilizer provided by the present invention is also added with brassinolide and sophorolipids, which can further improve the effect of the fertilizer.

[0041] Example 3

[0042] This embodiment provides the application of composite agricultural bacterial fertilizer in apple planting.

[0043] The experiment was conducted in an apple plantation in Lingbao City, Henan Province. The apple variety used was Fuji apple, which was 6 years old and planted at a spacing of 3m×4m. The formulation of the agricultural compound bacterial fertilizer used in the experiment was water dispersible granules, and the preparation method was the same as in Example 1.

[0044] The experiment involved 12 treatments in total, namely, experiment 13: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 12:5:8; experiment 14: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 16:4:6; experiment 15: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 20:3:4; experiment 16: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 25:2:3; experiment 17: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was 28:1:2; experiment 18: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as experiment 13, The Bacillus Velez was replaced by Bacillus megaterium in equal amounts; Experiment 19: Bacillus subtilis was not added; Experiment 20: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as that in Experiment 13, and the sophorolipid was replaced by lecithin in equal amounts; Experiment 21: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as that in Experiment 13, and the sophorolipid was replaced by lauric acid in equal amounts; Experiment 22: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as that in Experiment 13, and sophorolipid was not added; Experiment 23: the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus Velez was the same as that in Experiment 13, and brassinolide was not added; Experiment 24: the control group, no agricultural compound bacterial fertilizer was applied.

[0045] The randomized block arrangement was used with three replicates, and the area of ​​each plot was 120 m 2 The compound agricultural fertilizer was diluted 600 times and irrigated the roots of Fuji apple plants on June 18 and August 12, with 3000 mL per plant, and 0.2 wt% potassium dihydrogen phosphate solution was sprayed on the leaves at 70 mL / 90 m 2 , and the management after application was the same as that of the field. At the fruit maturity stage, 50 fruit samples with the same maturity were collected from different positions of the 5 fruit trees in each treatment, and the fruit yield, edible rate, soluble solid content, soluble total sugar content, titratable acid and coloring rate were statistically analyzed. The statistical results are shown in Table 2.

[0046] Table 2: Effects of compound agricultural bacterial fertilizer on yield and quality of Fuji apples

[0047]

[0048]

[0049] As shown in Table 1, the composite agricultural bacterial fertilizer provided by the present invention has a promoting effect on the yield, quality and coloring rate of Fuji apples. After applying the composite agricultural bacterial fertilizer, the yield of Fuji apples increased to 688.02-693.37 kg / 120 m 2, compared with the control group, the yield increased by 8.44-9.28%; the soluble solid content increased from 13.3% to 14.3-14.5%; the soluble total sugar content increased from 11.17% to 12.25-12.34%; the titratable acidity decreased from 0.33% to 0.20-0.21%; the coloring rate increased from 70.23% to 80.45-84.06%; when the mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velezii was 16:4:6, the yield, quality and coloring of Fuji apples were the best. It can be seen that the yield, quality and coloring rate of Fuji apples were improved after applying the composite agricultural fertilizer provided by the present invention. After exceeding the ratio of the composite agricultural fertilizer provided by the present invention, the improvement effect on the yield, quality and coloring rate of Fuji apples is poor; the composite agricultural fertilizer provided by the present invention is also added with brassinolide and sophorolipids, which can further improve the effect of the fertilizer.

[0050] As described above, the basic principle, main features and advantages of the present invention are well described. The above embodiments and descriptions are only descriptions of the preferred embodiments of the present invention, and the present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, various changes and improvements made by ordinary technicians in this field to the technical solution of the present invention should fall within the protection scope determined by the present invention.

Claims

1. A composite agricultural bacterial fertilizer, It is characterized in that Active ingredients include: Bacillus subtilis, Bacillus licheniformis and Bacillus velez; The effective ingredients of the composite agricultural bacterial fertilizer also include brassinolide and sophorolipids; The effective number of live bacteria in the composite agricultural fertilizer is ≥2.0×10 10 CFU / g.

2. The composite agricultural bacterial fertilizer according to claim 1, It is characterized in that The mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velez is 12-25:2-5:3-8.

3. The composite agricultural bacterial fertilizer according to claim 1, It is characterized in that The mass ratio of Bacillus subtilis, Bacillus licheniformis and Bacillus velez was 16:4:

6.

4. The composite agricultural bacterial fertilizer according to claim 1, It is characterized in that In terms of mass percentage, the added amount of brassinolide is 0.03%; In terms of mass percentage, the amount of sophorolipid added is 0.2%.

5. Application of the composite agricultural bacterial fertilizer according to any one of claims 1 to 4 in increasing crop yields, It is characterized in that The crops include plums and apples.

6. Use of the composite agricultural bacterial fertilizer according to any one of claims 1 to 4 in improving the quality of crops, It is characterized in that The method of improving the quality of crops includes improving the edible rate of crops, increasing the soluble solid content, increasing the soluble total sugar content and reducing the titratable acidity.

7. Use of the composite agricultural bacterial fertilizer according to any one of claims 1 to 4 in improving fruit coloring.

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