Novel thallus fermentation process and equipment

Abstract

The invention relates to the technical field of thallus fermentation, and discloses a novel thallus fermentation process and equipment, and the novel thallus fermentation process comprises the following steps: S1, forming an aqueous two-phase system in a fermentation medium, the aqueous two-phase system comprises polyethylene glycol and phosphate, the concentration of PEG is 15% w / w, and the concentration of phosphate is 10% w / w; s2, inoculating a microbial strain into the culture medium, and controlling fermentation conditions including the temperature of 30 DEG C, the pH value of 6.57.0 and the dissolved oxygen content of 4060%; s3, separating the PEG phase from the phosphate phase through a semi-permeable membrane system in the fermentation process, and removing a target product in the PEG phase in real time through a pump circulation system; and S4, collecting the product, separating out the target product from the PEG phase by cooling or diluting, and recovering the PEG and the phosphate phase for recycling. By improving fermentation efficiency and product purity and reducing production cost and resource waste, the problems of low fermentation efficiency, low product purity and high cost in the prior art are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial fermentation, in particular to a novel bacterial fermentation process and equipment. Background Art

[0002] Bacterial fermentation technology has a wide range of applications in biopharmaceuticals, food industry, and bioenergy, especially for the production of high-value biological products such as antibiotics, enzymes, drug precursors, and vitamins. However, with the increasing demand for these products, how to improve fermentation efficiency and product purity and reduce production costs has become a key technical challenge.

[0003] In the existing bacterial fermentation process, a single fermentation process is adopted, and the product is directly accumulated in the fermentation broth. This method has obvious limitations: as the product concentration increases, the target product in the fermentation broth will have an inhibitory effect on the bacteria, resulting in reduced fermentation efficiency. In addition, after the fermentation is completed, complex separation and purification steps are required to improve the purity of the product, which increases production time and cost. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a novel bacterial fermentation process and equipment, which solves the problems of low fermentation efficiency and low product purity caused by product accumulation in the prior art.

[0005] To achieve the above objectives, the present invention is implemented by the following technical scheme: a novel bacterial fermentation process comprises the following steps:

[0006] a. forming a two-phase aqueous system in the fermentation medium of the reactor, wherein the two-phase aqueous system consists of polyethylene glycol and phosphate, wherein the concentration of PEG is 15% w / w and the concentration of phosphate is 10% w / w;

[0007] b. inoculating the microbial strain into the above culture medium and controlling the fermentation conditions, including a temperature of 30° C., a pH value between 6.5 and 7.0, and a dissolved oxygen content of 40 to 60%;

[0008] c. During the fermentation process, the PEG phase is separated from the phosphate phase by a semipermeable membrane system, and the target product in the PEG phase is removed in real time by a pump circulation system;

[0009] d. Collect the product and precipitate the target product from the PEG phase by cooling or diluting, and recover the PEG and phosphate phases for recycling.

[0010] Preferably, the phase partition coefficient K of the two-phase aqueous system is expressed by the following formula:

[0011]

[0012] Among them, C PEGis the concentration of the product in the PEG phase, C 磷酸盐 is the concentration of the product in the phosphate phase. The K value is optimized by adjusting the molecular weight and concentration of PEG to make the K value between 2 and 5 to increase the enrichment of the product in the PEG phase.

[0013] Preferably, the extraction efficiency E of the target product during the fermentation process is expressed by the following formula:

[0014]

[0015] Among them, V PEG is the volume of the PEG phase, V 发酵液 is the volume of the fermentation broth, C PEG is the concentration of the target product in the PEG phase, C 发酵液 is the concentration of the target product in the fermentation broth, and the extraction efficiency E is controlled between 60% and 90% to optimize the product collection process.

[0016] Preferably, the reactor is designed as an integrated reactor, including a fermentation chamber and a two-phase aqueous separation chamber, wherein the fermentation chamber and the separation chamber are connected via a semipermeable membrane, and the pore size of the semipermeable membrane ranges from 0.1 to 0.5 microns, which is used to effectively prevent bacteria and macromolecular impurities from entering the separation chamber.

[0017] Preferably, during the fermentation process, the phase volume ratio V of the two-phase aqueous system is controlled PEG :V 发酵液 The ratio is between 1:1 and 1:2, and the system operating temperature is between 25°C and 30°C to ensure the stability and efficiency of phase separation.

[0018] Preferably, the system is equipped with online monitoring equipment for real-time monitoring of product concentration, pH value, temperature and dissolved oxygen content in the fermentation broth, and automatically adjusting key parameters in the fermentation and extraction process through a data acquisition and control system to ensure the continuity and efficiency of the production process.

[0019] New bacterial fermentation equipment, including:

[0020] The fermentation tank is used to contain fermented materials, and a feed port is fixedly connected to the top of one side of the fermentation tank for feeding raw materials;

[0021] The discharge port is arranged at the bottom of the fermentation tank and is used to discharge the fermented materials;

[0022] A fixed plate, fixed to the outer periphery of the fermentation tank, used to support the fermentation tank and in contact with the ground through a plurality of evenly distributed support rods at the bottom;

[0023] A fixed frame, fixed on the top of the fermentation tank, used to support the stirring mechanism, and a motor is connected to the top of the fixed frame to drive the stirring device;

[0024] A rotating shaft 1 is connected to the driving end of the motor and is used to drive the rotating plate to rotate;

[0025] The rotating plate is stably connected to the fixed frame and the top of the fermentation tank by rotating the connected crankshaft, and is used to drive the stirring blades to stir the materials;

[0026] A crankshaft, rotatably connected to the fixed frame and the top side of the fermentation tank through a stabilizing block, for connecting a stirring blade;

[0027] The belt pulley is arranged on the outer periphery of the first rotating shaft and connected to the second rotating shaft through a belt for transmitting power;

[0028] Gear 1 and gear 2 are respectively fixed on the rotating shaft 2 and the outer periphery of the crankshaft, and are connected by gear meshing to synchronously rotate the stirring device.

[0029] Preferably, a pressure sensor is fixedly connected to one side of the fermentation tank for monitoring the pressure inside the tank, and an air inlet is opened inside the front side of the fermentation tank for gas to enter the fermentation tank.

[0030] Preferably, one side of the fermentation tank is connected to an air pump through a connecting pipe to provide gas flow, and the output and input ends of the air pump are fixedly connected to an air pipe, one end of the air pipe is connected to the top side of the fermentation tank, and the other end is connected to a filter box to discharge waste gas.

[0031] Preferably, a temperature sensor is fixedly connected to one side of the fermentation tank for detecting the fermentation temperature, and a pH sensor is fixedly connected to detect the change of pH value during the fermentation process. A plurality of evenly distributed heaters are connected to the periphery of the fermentation tank by welding for controlling the fermentation temperature.

[0032] Working principle: culture medium and other nutrients can be placed inside the fermentation tank through the feed port, the starting motor can drive shaft one to rotate, shaft one can drive the rotating plate and belt pulley to rotate, the rotating plate can drive the crankshaft to rotate, the belt pulley can drive shaft two to rotate, shaft two can drive gear one to rotate, gear one can drive gear two to rotate, gear two can drive the crankshaft to rotate, the crankshaft can drive the stirring blades to rotate, the crankshaft and stirring blades can ensure uniform mixing of culture medium and bacteria through rotation, promote the distribution of oxygen and nutrients, avoid precipitation, and improve the growth and metabolic efficiency of bacteria. The pressure sensor can detect the pressure inside the fermentation tank, and the gas can be injected into the fermentation tank through the air inlet. Starting the air pump can discharge the gas from the inside of the fermentation tank to the inside of the filter box through the air pipe, and the gas is discharged by filtering through the filter plate. The fermentation process can be ensured to be stable by controlling the air pressure inside the fermentation tank. The temperature inside the fermentation tank can be detected by the temperature sensor, and the fermentation tank can be heated by the heater. The fermentation process can be ensured to be stable by controlling the temperature inside the fermentation tank. The pH value of the fermentation liquid can be measured in real time by the pH sensor, and the acidic liquid in the storage box can be automatically added to the fermentation liquid through the nozzle, so as to maintain the appropriate pH value of the fermentation liquid and ensure that the pH value of the culture liquid is within a range suitable for bacterial growth.

[0033] The present invention provides a novel bacterial fermentation process and equipment, which has the following beneficial effects:

[0034] 1. By introducing a two-phase aqueous extraction system and optimized stirring technology, the present invention can remove the target product accumulated during the fermentation process in real time, reduce the inhibitory effect of the product on the growth of the bacteria, thereby significantly prolonging the fermentation time and increasing the product yield; at the same time, the extraction process helps to separate the product from impurities, reduce subsequent purification steps, and ultimately improve the purity of the product. This effect is particularly significant when producing high-value biological products, and can meet the industrial needs of efficient and high-quality production.

[0035] 2. The present invention can recycle PEG and phosphate phases in the two-phase aqueous system, which significantly reduces raw material consumption and production costs; in addition, the optimized process design reduces energy consumption and environmental burden, and helps to achieve a green and sustainable production process; through system monitoring and automatic adjustment technology, the stability and economy of the production process are further improved, ensuring efficient use of resources.

[0036] 3. The present invention starts the motor to drive the rotating shaft 1 to rotate, the rotating shaft 1 drives the rotating plate and the belt pulley to rotate, the rotating plate then drives the crankshaft to rotate, the belt pulley drives the rotating shaft 2 to rotate, the rotating shaft 2 further drives the gear 1 to rotate, the gear 1 drives the gear 2 to rotate, and finally the gear 2 drives the crankshaft to rotate, the rotation of the crankshaft drives the stirring blade to rotate, the crankshaft and the stirring blade can ensure the uniform mixing of the culture medium and the bacteria through rotation, promote the distribution of oxygen and nutrients, avoid precipitation, and improve the growth and metabolic efficiency of the bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 is a process flow chart of the present invention;

[0038] Figure 2 It is a three-dimensional schematic diagram of the present invention;

[0039] Figure 3 It is a schematic diagram of the structure of the fermentation tank of the present invention;

[0040] Figure 4 For the present invention Figure 3 The enlarged view of point A in the middle;

[0041] Figure 5 It is a schematic structural diagram of the filter box of the present invention.

[0042] Among them, 1. fermentation tank; 2. feed port; 3. discharge port; 4. valve switch; 5. fixed plate; 6. support rod; 7. fixed frame; 8. motor; 9. shaft 1; 10. rotating plate; 11. crankshaft; 12. stirring blade; 13. stabilizing block; 14. belt pulley; 15. shaft 2; 16. gear 1; 17. gear 2; 18. pressure sensor; 19. air inlet; 20. air pump; 21. air pipe; 22. filter box; 23. exhaust port; 24. filter plate; 25. temperature sensor; 26. pH sensor; 27. heater; 28. storage box; 29. ​​nozzle. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Please refer to the attached Figure 1 - Attachment Figure 5 The novel bacterial fermentation process of the present invention comprises the following steps:

[0045] Preparation of fermentation medium: PEG (molecular weight 4000-8000, concentration 15% w / w) and phosphate (concentration 10% w / w) were added to the fermentation medium and mixed evenly to form a two-phase aqueous system.

[0046] Inoculation and fermentation: Inoculate the optimized microbial strain (such as genetically engineered antibiotic-producing bacteria) into the above culture medium. Control the fermentation temperature to 30°C, the pH value to 6.5-7.0, and the dissolved oxygen content to 40-60%.

[0047] Agitation and extraction: During the fermentation process, a stirring system (agitation rate of 100-300 rpm) is used to maintain the uniform distribution of the two-phase aqueous system. At the same time, the PEG phase is separated from the phosphate phase by a semipermeable membrane separation system, and the PEG phase rich in the target product is removed in real time by a pump system.

[0048] Product separation and recovery: The extracted PEG phase is separated by cooling or dilution, the target product is precipitated from the PEG phase, and the recovered PEG and phosphate phases are continuously recycled.

[0049] Equipment design:

[0050] Fermentation reactor: The reactor is equipped with a fermentation chamber and a separation chamber, which are connected by a semipermeable membrane. The stirring device is located in the fermentation chamber to ensure uniform mixing of the fermentation liquid and the extraction phase.

[0051] Semipermeable membrane system: Use a semipermeable membrane with a pore size of 0.1 to 0.5 microns to ensure that bacteria and large molecular impurities do not enter the separation chamber.

[0052] Stirring system: The stirrer is made of stainless steel, with three or four blades and adjustable stirring rate to ensure the best mass transfer effect.

[0053] Example 1: Antibiotic production

[0054] 1.1 Fermentation conditions

[0055] Strain: An antibiotic-producing strain that has been genetically engineered to have the ability to produce high levels of antibiotics.

[0056] Culture medium: contains 20g / L glucose, 10g / L yeast extract, 5g / LNaCl, 5g / LK2HPO4, 0.1g / LMgSO4.

[0057] Two-phase aqueous system: PEG4000 was used at a concentration of 15% w / w, phosphate concentration was 10% w / w, and the phase partition coefficient K was controlled at 3.5.

[0058] Fermentation conditions: temperature 30°C, pH 6.8, dissolved oxygen content maintained at 45%, and stirring rate of 200 rpm.

[0059] Extraction and product collection: During the fermentation process, the PEG phase and the phosphate phase are separated by a semipermeable membrane system, and the antibiotic-rich PEG phase is removed using a pump system and cooled at 10°C to isolate high-purity antibiotics.

[0060] 1.2 Experimental Results

[0061] Product yield: Through the extraction process, the antibiotic yield increased by 30%, from 500 mg / L to 650 mg / L.

[0062] Purity: The purity of the antibiotics reached 95% as tested by high performance liquid chromatography (HPLC), which is 15% higher than that of the traditional process.

[0063] Comparative Example 1: Traditional fermentation process without extraction system

[0064] 1.1 Fermentation conditions

[0065] The strain, culture medium and fermentation conditions were the same as those in Example 1, but the aqueous two-phase extraction system was not used.

[0066] 1.2 Experimental Results

[0067] Product yield: Antibiotic yield is 500 mg / L.

[0068] Purity: The purity of the antibiotic tested by HPLC is only 80%.

[0069] Analysis: By comparison, the aqueous two-phase extraction process in Example 1 significantly improved the yield and purity of antibiotics.

[0070] Example 2: Enzyme production

[0071] 2.1 Fermentation conditions

[0072] Strain: Use an engineered industrial enzyme production strain that has been screened and optimized to efficiently express the target enzyme during the fermentation process.

[0073] Culture medium: contains 30g / L glucose, 10g / L yeast extract, 5g / L(NH4)2SO4, 3g / LKH2PO4, 0.5g / LMgSO4.

[0074] Two-phase aqueous system: PEG6000 was used at a concentration of 12% w / w, phosphate concentration was 8% w / w, and the phase partition coefficient K was controlled at 4.0.

[0075] Fermentation conditions: temperature 28°C, pH 7.0, dissolved oxygen content maintained at 50%, and stirring rate of 250 rpm.

[0076] Extraction and product collection: During the fermentation process, the enzyme is extracted through a two-phase aqueous system, the enzyme is enriched in the PEG phase, and high-purity enzyme is precipitated by cooling.

[0077] 2.2 Experimental Results

[0078] Product yield: The total enzyme yield was 700 U / mL, which was 25% higher than that of the traditional process (560 U / mL).

[0079] Enzyme purity: The purity of the enzyme is 90%, which can be further increased to more than 95% after purification steps.

[0080] Comparative Example 2: Fermentation in a single aqueous phase system

[0081] 2.1 Fermentation conditions

[0082] The strain, culture medium and fermentation conditions were the same as those in Example 2, but the aqueous two-phase extraction system was not used.

[0083] 2.2 Experimental Results

[0084] Product yield: The total enzyme production was 560 U / mL.

[0085] Enzyme Purity: The purity of the enzyme is 70%.

[0086] Analysis: The aqueous two-phase extraction process significantly improved the yield and purity of the enzyme and is suitable for industrial applications requiring high-purity enzymes.

[0087] Example 3: Prodrug Production

[0088] 3.1 Fermentation conditions

[0089] Strain: A strain of microorganism that has been genetically engineered to produce a specific drug precursor.

[0090] Culture medium: contains 25g / L glucose, 8g / L yeast extract, 7g / L(NH4)2SO4, 5g / LK2HPO4, and 0.2g / LMgSO4.

[0091] Two-phase aqueous system: PEG8000 was used at a concentration of 18% w / w, phosphate concentration was 12% w / w, and the phase partition coefficient K was controlled at 4.5.

[0092] Fermentation conditions: temperature 32°C, pH 6.5, dissolved oxygen content maintained at 50%, and stirring rate of 150 rpm.

[0093] Extraction and product collection: During the fermentation process, the drug precursor is extracted in real time through a two-phase aqueous system, and a high-purity product is precipitated by cooling.

[0094] 3.2 Experimental Results

[0095] Product yield: The total yield of drug precursor is 400 mg / L, which is 33% higher than the traditional process (300 mg / L).

[0096] Purity: The purity of the prodrug is 98% and little further purification is required.

[0097] Comparative Example 3: Low stirring rate conditions

[0098] 3.1 Fermentation conditions

[0099] The strain, culture medium and fermentation conditions were the same as in Example 3, but the stirring rate was reduced to 50 rpm.

[0100] 3.2 Experimental Results

[0101] Product yield: The total yield of the drug precursor was 280 mg / L, a decrease of 30%.

[0102] Purity: The purity of the prodrug was 85%.

[0103] Analysis: The stirring rate has a significant effect on the fermentation and extraction processes. Appropriate stirring rate can improve mass transfer efficiency, thereby increasing product yield and purity.

[0104] Example 4: Biofuel ethanol production

[0105] 4.1 Fermentation conditions

[0106] Strain: Use industrial yeast strains, which are used for the production of ethanol.

[0107] Culture medium: contains 50g / L glucose, 10g / L yeast extract, 5g / LKH2PO4, 1g / LMgSO4.

[0108] Two-phase aqueous system: PEG4000 was used at a concentration of 10% w / w, phosphate concentration was 7% w / w, and the phase partition coefficient K was controlled at 2.5.

[0109] Fermentation conditions: temperature 30°C, pH 5.5, dissolved oxygen content maintained at 45%, and stirring rate 180 rpm.

[0110] Extraction and product collection: During the fermentation process, ethanol is extracted in real time and high-purity ethanol is precipitated by cooling.

[0111] 4.2 Experimental Results

[0112] Product yield: The ethanol yield is 110 g / L, which is 22% higher than the traditional process (90 g / L).

[0113] Purity: The purity of ethanol is 96%, and the purified ethanol can be used for fuel purposes.

[0114] Comparative Example 4: No stirring system conditions

[0115] 4.1 Enzyme conditions

[0116] The strain, culture medium and fermentation conditions were the same as those in Example 4, but no stirring system was used.

[0117] 4.2 Experimental Results

[0118] Product yield: The ethanol yield was 85 g / L, a decrease of 23%.

[0119] Purity: The purity of ethanol is 75%.

[0120] Analysis: The stirring system significantly improves the mass transfer efficiency and ensures efficient production of ethanol.

[0121] Example 5: Vitamin production

[0122] 5.1 Fermentation conditions

[0123] Strains: Use engineered bacterial strains for the production of vitamin B12.

[0124] Culture medium: contains 40g / L glucose, 15g / L yeast extract, 10g / L(NH4)2SO4, 3g / LKH2PO4, 0.5g / LMgSO4.

[0125] Two-phase aqueous system: PEG6000 was used at a concentration of 14% w / w, phosphate concentration was 9% w / w, and the phase partition coefficient K was controlled at 3.8.

[0126] Fermentation conditions: temperature 35°C, pH 7.2, dissolved oxygen content maintained at 50%, and stirring rate of 220 rpm.

[0127] Extraction and product collection: During the fermentation process, vitamin B12 is extracted through a two-phase aqueous system, and a high-purity product is precipitated by cooling.

[0128] 5.2 Experimental Results

[0129] Product yield: The yield of vitamin B12 is 35 mg / L, which is 40% higher than the traditional process (25 mg / L).

[0130] Purity: The purity of vitamin B12 is 97%, which can reach over 99% after further purification.

[0131] Comparative Example 5: Non-aqueous two-phase extraction system

[0132] 5.1 Enzyme conditions

[0133] The strain, culture medium and fermentation conditions were the same as those in Example 5, but the aqueous two-phase extraction system was not used.

[0134] 5.2 Experimental Results

[0135] Product yield: The yield of vitamin B12 was 25 mg / L, which was reduced by 28%.

[0136] Purity: The purity of vitamin B12 is 80%.

[0137] Experimental example: Correlation test between stirring rate and product yield and purity

[0138] 6.1 Experimental design

[0139] The drug precursor production effect in Example 3 was tested at different stirring rates, and the stirring rates were set to 50 rpm, 100 rpm, 150 rpm, 200 rpm and 250 rpm, respectively.

[0140] 6.2 Experimental Results

[0141] Stirring rate: 50 rpm: product yield: 280 mg / L, purity: 85%.

[0142] Stirring rate: 100 rpm: product yield: 320 mg / L, purity: 90%.

[0143] Stirring rate: 150 rpm: product yield: 350 mg / L, purity: 93%.

[0144] Stirring rate 200 rpm: product yield 400 mg / L, purity 98%.

[0145] Stirring rate: 250 rpm: product yield: 370 mg / L, purity: 95%.

[0146] According to the above content, the stirring rate has a significant effect on the yield and purity of the product. An appropriate stirring rate (such as 150-200 rpm) can maximize the yield and purity of the product, while an excessively high stirring rate may cause excessive shear force, damage the bacteria, and reduce the product yield.

[0147] The following is a table that organizes the embodiments, comparative examples and experimental examples to more clearly show the conditions and results of each experiment.

[0148] Table 1: Experimental data of examples and comparative examples

[0149]

[0150]

[0151] Table 1 summarizes the experimental conditions and results of each embodiment and comparative example, and clarifies the changes in the yield and purity of the product under different conditions.

[0152] Table 2: Effect of stirring rate on product yield and purity in experimental examples

[0153] Experiment number Stirring speed (rpm) Product yield (mg / L) purity(%) 6 50 280 85 6 100 320 90 6 150 350 93 6 200 400 98 6 250 370 95

[0154] Table 2 shows the effects of different stirring rates on product yield and purity in the experimental examples, illustrating the importance of optimizing the stirring rate for improving fermentation and extraction effects.

[0155] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A novel bacterial fermentation process, characterized in that: The following steps are involved: a. forming a two-phase aqueous system in the fermentation medium of the reactor, wherein the two-phase aqueous system consists of polyethylene glycol and phosphate, wherein the concentration of PEG is 15% w / w and the concentration of phosphate is 10% w / w; b. Inoculate the microbial strain into the above culture medium and control the fermentation conditions, including the temperature at 30° C. The pH value is between 6.5-7.0 and the dissolved oxygen content is maintained at 40-60%; c. During the fermentation process, the PEG phase is separated from the phosphate phase by a semipermeable membrane system, and the target product in the PEG phase is removed in real time by a pump circulation system; d. Collect the product and precipitate the target product from the PEG phase by cooling or diluting, and recover the PEG and phosphate phases for recycling.

2. The novel bacterial fermentation process according to claim 1, characterized in that: The phase partition coefficient K of the two-phase aqueous system is expressed by the following formula: Among them, C PEG is the concentration of the product in the PEG phase, C 磷酸盐 is the concentration of the product in the phosphate phase. The K value is optimized by adjusting the molecular weight and concentration of PEG to make the K value between 2 and 5 to increase the enrichment of the product in the PEG phase.

3. The novel bacterial fermentation process according to claim 1, characterized in that: The extraction efficiency E of the target product in the fermentation process is expressed by the following formula: Among them, V PEG is the volume of the PEG phase, V 发酵液 is the volume of the fermentation broth, C PEG is the concentration of the target product in the PEG phase, C 发酵液 is the concentration of the target product in the fermentation broth, and the extraction efficiency E is controlled between 60% and 90% to optimize the product collection process.

4. The novel bacterial fermentation process according to claim 1, characterized in that: The reactor is designed as an integrated reactor, including a fermentation chamber and a two-phase aqueous separation chamber. The fermentation chamber and the separation chamber are connected through a semipermeable membrane. The pore size of the semipermeable membrane ranges from 0.1 to 0.5 microns, which is used to effectively prevent bacteria and macromolecular impurities from entering the separation chamber.

5. The novel bacterial fermentation process according to claim 1, characterized in that: During the fermentation process, the volume ratio V of the two-phase aqueous system is controlled. PEG :V 发酵液 The ratio is between 1:1 and 1:2, and the system operating temperature is between 25°C and 30°C to ensure the stability and efficiency of phase separation.

6. The novel bacterial fermentation process according to claim 1, characterized in that: The system is equipped with online monitoring equipment for real-time monitoring of product concentration, pH value, temperature and dissolved oxygen content in the fermentation broth, and automatically adjusts key parameters in the fermentation and extraction processes through a data acquisition and control system to ensure the continuity and efficiency of the production process.

7. A novel bacterial fermentation equipment, characterized in that: The novel bacterial fermentation process according to any one of claims 1 to 6, comprising: A fermentation tank (1) is used to contain fermentation materials, and a feed inlet (2) is fixedly connected to the top of one side of the fermentation tank for feeding raw materials; A discharge port (3) is provided at the bottom of the fermentation tank (1) and is used to discharge the fermented material; A fixed plate (5), fixed on the outer periphery of the fermentation tank (1), used to support the fermentation tank, and in contact with the ground through a plurality of evenly distributed support rods (6) at the bottom; A fixed frame (7), fixed on the top of the fermentation tank (1), used to support the stirring mechanism, and a motor (8) is connected to the top of the fixed frame to drive the stirring device; A rotating shaft (9) connected to the driving end of the motor (8) and used to drive the rotating plate (10) to rotate; The rotating plate (10) is stably connected to the fixed frame (7) and the top of the fermentation tank (1) through a rotatably connected crankshaft (11), and is used to drive the stirring blades (12) to stir the material; A crankshaft (11) is rotatably connected to the fixed frame (7) and the top side of the fermentation tank (1) through a stabilizing block (13) and is used to connect a stirring blade (12); A belt pulley (14) is arranged on the outer periphery of the first rotating shaft (9) and is connected to the second rotating shaft (15) via a belt for transmitting power; Gear 1 (16) and gear 2 (17) are respectively fixed on the outer periphery of rotating shaft 2 (15) and crankshaft (11), and are connected by gear meshing to synchronously rotate the stirring device.

8. The novel bacterial fermentation equipment according to claim 7, characterized in that: A pressure sensor (18) is fixedly connected to one side of the fermentation tank (1) for monitoring the pressure inside the tank, and an air inlet (19) is opened inside the front side of the fermentation tank (1) for gas to enter the fermentation tank.

9. The novel bacterial fermentation equipment according to claim 7, characterized in that: One side of the fermentation tank (1) is connected to an air pump (20) via a connecting pipe for providing gas flow, and the output end and input end of the air pump (20) are both fixedly connected to an air pipe (21), one end of the air pipe (21) is connected to the top side of the fermentation tank (1), and the other end is connected to a filter box (22) for discharging waste gas.

10. The novel bacterial fermentation equipment according to claim 7, characterized in that: A temperature sensor (25) is fixedly connected to one side of the interior of the fermentation tank (1) for detecting the fermentation temperature, and a pH sensor (26) is fixedly connected to the other side for detecting changes in pH value during the fermentation process. A plurality of evenly distributed heaters (27) are connected to the periphery of the fermentation tank (1) by welding for controlling the fermentation temperature.

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