Method for cultivating tissue culture seedlings of rubber trees through one-step seedling culture

Abstract

The invention relates to the technical field of woody plant planting, and particularly provides a rubber tree tissue culture seedling cultivation method adopting a one-step method. The method comprises the following steps: (1) mixing the root of a rubber tree tissue culture seedling with an arabinose / IAA composite solution; (2) planting the hevea brasiliensis tissue culture seedlings obtained in the step (1) in a greenhouse; (3) after planting, fertilizing the tissue culture seedlings of the rubber trees; after planting, a sunshade net is arranged above the rubber tree tissue culture seedlings. Compared with the prior art, according to the one-step seedling raising method, seedling emergence from a laboratory to seedling outplanting can be completed only in five months, the seedling raising time is greatly shortened, root systems are developed, the seedling quality is improved, and the outplanting rate reaches 100%; the method disclosed by the invention is simple to operate, relatively low in cost and suitable for practical production and application, and can provide technical support for large-scale breeding of the tissue culture seedlings of the rubber trees.

Description

Technical Field

[0001] The invention relates to the technical field of woody plant planting, and more particularly to a one-step seedling cultivation method for rubber tree tissue culture seedlings. Background Art

[0002] Since the advent of rubber tree tissue culture seedlings, they have been highly concerned by all walks of life due to their advantages such as fast growth and high yield. The current somatic embryo cycle proliferation and plant regeneration are the main proliferation methods. After the rubber tree tissue culture seedlings emerge from the weak light, constant temperature and humidity, sterile tissue culture room, the seedlings are weak and sensitive to temperature, humidity and light. At the same time, the young roots, stems and leaves are easily invaded by pathogens, and the mortality rate is high.

[0003] For many years, the existing seedling raising method is a two-step seedling raising method, which is to raise test tube seedlings from the tissue culture room, acclimate them on the sand bed for 3-5 months, and then place them in nutrient bags for 6-10 months. After they grow to 50-130cm, they are planted in the nursery. This process involves two seedling raisings, and each time the seedlings are damaged, resulting in a decrease in the seedling success rate. The procedures are cumbersome, the cycle is long, and a lot of manpower is consumed, resulting in an increase in seedling raising costs. It takes one year from the laboratory to the nursery. In commercial applications, there are risks such as long contract cycles and unstable price systems. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a one-step method for cultivating rubber tree tissue culture seedlings. The one-step seedling cultivation method of the present invention can complete the process from seedling emergence in the tissue culture laboratory to seedling emergence in the nursery in only 5 months, which greatly shortens the seedling cultivation time, reduces commercial risks, saves costs, enhances seedling consistency, increases emergence rate, reduces root damage, forms a developed root system, and can improve the survival rate of field transplantation, thereby providing the industry with more high-quality varieties and seedlings.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A method for cultivating rubber tree tissue culture seedlings by one-step seedling cultivation comprises the following steps:

[0007] (1) mixing the roots of rubber tree tissue culture seedlings with the arabinose / IAA composite solution;

[0008] (2) planting the rubber tree tissue culture seedlings obtained in step (1) in a greenhouse;

[0009] (3) after planting, fertilizing the rubber tree tissue culture seedlings;

[0010] After planting, a film shed and sunshade net are set up above the rubber tree tissue culture seedlings;

[0011] After 5 days of planting, cover half of the shade net; after 7 days of planting, cover half of the shade net from 10 am to 4 pm, and remove the shade net during the remaining time; after 10 days of planting, remove the shade net and film arch shed.

[0012] Preferably, after 5 days of planting, the film is gradually opened to cover half of the sunshade net; after 7 days of planting, half of the sunshade net is covered from 10 am to 4 pm, and the sunshade net is removed during the remaining time period; after 10 days of planting, the film and sunshade net are removed. After about 45 days of greenhouse planting (when the new leaf canopy is in a stable aging state), the seedlings can be moved to an ordinary sunshade shed (with a shading rate of about 70%) to continue hardening. At the same time, 1 or 2 plants / row are arranged in sparse rows to reduce the ratio of weak plants. Water and fertilizer are applied every 15 days. After the second canopy of new leaves grows and ages stably, the sunshade is removed to keep the seedlings hardened in full sunlight. After 5 months of outdoor shade shed seedling cultivation, the plant height reaches more than 120 cm, and the leaves on the top canopy are stable and can be transplanted.

[0013] Preferably, the rubber tree tissue culture seedlings are planted in a seedling medium; the seedling medium is made by mixing Danish Pindstrup Sphagnum and vermiculite; the volume ratio of Danish Pindstrup Sphagnum to vermiculite is (5-9): 1. The Danish Pindstrup Sphagnum and vermiculite in the above ratio can have good water retention and air permeability.

[0014] Preferably, before planting rubber tree tissue culture seedlings, the seedling substrate is disinfected; the disinfecting reagents include carbendazim, wettable powder and avermectin; the disinfection includes: preparing a mixed disinfectant with 800 times of carbendazim, 1500 times of 50% wettable powder and 1000 times of avermectin, mixing the mixed disinfectant with the seedling substrate, covering with a film and exposing to the sun for 10 days.

[0015] Preferably, in the arabinose / IAA (auxin) composite solution, the concentration of arabinose is 5 to 10 wt %;

[0016] In the arabinose / IAA (auxin) composite solution, the concentration of IAA (auxin) is 100-200 mg / L.

[0017] Preferably, after planting, the rubber tree tissue culture seedlings have a plant spacing of 5-6 cm and a row spacing of 8-10 cm.

[0018] Preferably, the fertilization is carried out 7 to 40 days after planting the rubber tree tissue culture seedlings; the fertilization includes 6-8 fertilizations; the first fertilization is carried out on the 7th to 9th day after planting the rubber tree tissue culture seedlings, the second fertilization is carried out on the 21st to 24th day after planting the rubber tree tissue culture seedlings, and the third fertilization is carried out on the 36th to 40th day after planting the rubber tree tissue culture seedlings; subsequently, fertilization is carried out every 15 to 20 days.

[0019] Preferably, the fertilization specifically comprises: mixing nitrogen fertilizer, phosphate fertilizer, potash fertilizer and water to obtain a mixed solution, and fertilizing the rubber tree tissue culture seedlings with the mixed solution; in the mixed solution, the total content of nitrogen fertilizer, phosphate fertilizer and potash fertilizer is 0.3-0.5wt%; the nitrogen fertilizer, phosphate fertilizer and potash fertilizer are prepared into a 0.5-1% water-fertilizer solution according to N:P2O5:K2O=(15-21):4:11. A concentration of 0.5% is used in the greenhouse seedling canopy, and a concentration of 1% is used in the sunshade canopy outside the greenhouse.

[0020] Preferably, the nitrogen fertilizer includes urea; the phosphorus fertilizer includes superphosphate; and the potash fertilizer includes potassium chloride.

[0021] Preferably, the rubber tree tissue culture seedling cultivation method of one-step seedling cultivation further comprises the following steps:

[0022] 50 to 120 days after the rubber tree tissue culture seedlings are planted, topdressing is performed on the rubber tree tissue culture seedlings; the topdressing specifically includes: 50 days after the rubber tree tissue culture seedlings are planted, the first topdressing is performed, and then topdressing is performed once every 15 days.

[0023] Preferably, the topdressing specifically comprises: mixing nitrogen fertilizer, phosphate fertilizer, potash fertilizer and water to obtain a water fertilizer liquid, and topdressing the rubber tree tissue culture seedlings with the water fertilizer liquid; the total content of nitrogen fertilizer, phosphate fertilizer and potash fertilizer in the water fertilizer liquid is 0.5-1.0wt%. The nitrogen fertilizer, phosphate fertilizer and potash fertilizer are mixed with water in a mass ratio of N:P2O5:K2O=(15-21):4:11.

[0024] Preferably, the nitrogen fertilizer includes urea; the phosphorus fertilizer includes superphosphate; and the potash fertilizer includes potassium chloride.

[0025] Preferably, after topdressing twice, the shade net is removed and the seedlings are acclimatized and hardened under full sun.

[0026] Preferably, 50 days after planting, the rubber tree tissue culture seedlings are moved from the greenhouse to a shade shed; the shading rate of the shade shed is 50%.

[0027] Preferably, the rubber tree tissue culture seedling cultivation method of one-step seedling cultivation further comprises the following steps:

[0028] On the day of transplanting, the rubber tree tissue culture seedlings are placed on an elevated seedbed in a greenhouse, the bottom and surrounding of the seedbed are covered with sand, and 2 / 3 of the seedling bag is buried in the sand to keep moisture; the shading rate of the greenhouse is 80%.

[0029] Preferably, a spray device, a sunshade net, and a small arch shed are arranged in order from top to bottom above the rubber tree tissue culture seedlings; when the daytime temperature is higher than 28° C., spray to keep moist for 7 days; and remove the small arch shed 15 days after transplanting.

[0030] Compared with the prior art, the present invention has developed a one-step seedling raising method from test tube seedlings to bagged seedlings, which reduces the seedling raising process, improves the seedling emergence rate, saves labor, reduces the seedling cost, and shortens the seedling raising cycle. The one-step seedling raising method of the present invention can complete the process from laboratory emergence to seedling emergence in the nursery in only 5 months, and the emergence rate can reach 100%; the method of the present invention is simple to operate, low in cost, suitable for actual production application, and can provide technical support for the promotion and planting of rubber trees. DETAILED DESCRIPTION

[0031] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] To further illustrate the present invention, the following examples are used to provide a detailed description. The raw materials used in the following examples and comparative examples of the present invention are all commercially available products; arabinose was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a product number of B25845; mannose was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a product number of B21895.

[0033] Example 1

[0034] The rubber tree tissue culture seedling cultivation method of the one-step seedling raising of the present embodiment comprises the following steps:

[0035] (1) Prepare suitable seedling medium:

[0036] Danish Pindstrup Sphagnum and vermiculite are mixed in a volume ratio of 6:1 as a seedling medium. The above ratio of Danish Pindstrup Sphagnum and vermiculite can achieve both good water retention and aeration properties.

[0037] (2) Disinfection of seedling medium:

[0038] Pathogens are the main cause of seedling death. Therefore, on a sunny day, prepare a mixed disinfectant with 800 times carbendazim, 1500 times liquid of 50% wettable powder, and 1000 times avermectin (2% emulsion). Sprinkle it evenly on the substrate, cover it with a film and expose it to the sun for 10 days for disinfection and sterilization.

[0039] (3) Somatic embryo seedling transplanting:

[0040] The substrate was placed in a breathable non-woven seedling bag with a length of 30 cm. The roots of 10-15 cm tall tissue culture seedlings were soaked in a mixed aqueous solution containing 5 wt% arabinose and 100 mg / L IAA (auxin) for 0.5 h. The tissue culture seedlings were taken out and transplanted to the culture medium. Subsequently, the culture medium was transplanted into the bag. The plant spacing of each tissue culture seedling was 5-6 cm, the row spacing was 8-10 cm, and the rooting water was poured.

[0041] (4) Cooling and moisturizing:

[0042] On the day of transplanting, place the seedling bags of transplanted tissue culture seedlings on the elevated seedbed at the bottom of the greenhouse, cover the bottom and sides of the seedbed with sand, and bury 2 / 3 of the seedling bags in the sand to keep moisture; install the spray device, shade net, film, and small arch shed from top to bottom directly above the seedbed. When the daytime temperature is higher than 28℃, spray to keep moisture for 7 days. After the seedling slow-growing period and 15 days after transplanting, remove the small arch shed; the shading rate of the greenhouse is 80%.

[0043] (5) Photopic acclimation:

[0044] 5 days after transplanting, gradually open the film and uncover the shade net covering the lower half of the small shed to increase light; 7 days after transplanting, cover it from 10 am to 4 pm, and gradually remove the shade net after 10 days.

[0045] (6) Fertilization formula and method:

[0046] On the 7th day after transplanting, urea, superphosphate and potassium chloride were mixed in a mass ratio of N:P2O5:K2O=21:4:11 to obtain basal fertilizer, and the basal fertilizer was prepared into a 0.3wt% water-fertilizer and sprayed into the seedling bag; 15 days after the first fertilization, the basal fertilizer was prepared into a 0.5wt% water-fertilizer and applied, and then 0.5wt% water-fertilizer was applied every 15 days.

[0047] (7) Shade acclimation:

[0048] On the 10th day after transplanting, the film and the sunshade net were removed, and the seedling bags were raised in the seedbed for 50 days. After the new leaf buds were aged and stabilized (the leaves were dark green and hard), they were moved from the greenhouse to a conventional outdoor shade shed for domestication (with a shading rate of about 50%), and 1.0wt% of water fertilizer was applied every 15 days; the preparation method of the water fertilizer in this step included: urea, superphosphate, and potassium chloride were mixed according to a mass ratio of N:P2O5:K2O=15:4:11 to obtain a base fertilizer, and the base fertilizer was prepared into a 1.0wt% water fertilizer.

[0049] (8) Leave the field:

[0050] When the second new leaf is aging, open the sunshade net and carry out full-sun acclimatization and hardening. When the third new leaf is pulled out and the aging is stable, it can be planted in the nursery. The whole seedling raising cycle is 5 months.

[0051] Comparative Example 1

[0052] This comparative example refers to the preparation method in Example 1, except that in this comparative example, the arabinose in the mixed aqueous solution of step (3) is omitted, and the roots of the 10-15 cm tall tissue culture seedlings are soaked in an aqueous solution containing 100 mg / L IAAA (auxin) for 0.5 h; other treatments are carried out in accordance with Example 1.

[0053] Comparative Example 2

[0054] This comparative example refers to the preparation method in Example 1, except that in this comparative example, the arabinose in the mixed aqueous solution in step (3) is replaced by mannose; other treatments are carried out in accordance with Example 1.

[0055] Comparative Example 3

[0056] This comparative example refers to the preparation method in Example 1, except that steps (4) and (5) are omitted in this comparative example, and the seedling bags of transplanted tissue culture seedlings are placed on an elevated seedbed, the bottom and surrounding areas of the seedbed are covered with sand, 2 / 3 of the seedling bags are buried in the sand, and the bags are placed in a conventional outdoor shade shed for acclimatization (with a shading rate of about 50%); other treatments are carried out according to the steps in Example 1.

[0057] Comparative Example 4

[0058] This comparative example refers to the preparation method in Example 1, except that step (5) of this comparative example specifically includes:

[0059] Ten days after transplanting, uncover the lower half of the sunshade net in step (4) to increase light. Fifteen days after transplanting, cover the plants from 10 a.m. to 4 p.m., and gradually remove the sunshade net after 20 days.

[0060] The other treatments were carried out according to the steps in Example 1.

[0061] Comparative Example 5

[0062] This comparative example refers to the preparation method in Example 1, except that the urea in Example 1 is replaced by ammonium bicarbonate with the same nitrogen content; other treatments are carried out according to the steps in Example 1.

[0063] Comparative Example 6

[0064] This comparative example refers to the preparation method in Example 1, except that the superphosphate in Example 1 is replaced by calcium phosphate with the same phosphorus content; other treatments are carried out according to the steps in Example 1.

[0065] Comparative Example 7

[0066] This comparative example refers to the preparation method in Example 1, except that the potassium chloride in Example 1 is replaced by potassium nitrate with the same potassium content; other treatments are carried out according to the steps in Example 1.

[0067] Example 2

[0068] (1) Prepare suitable seedling medium:

[0069] Danish Pindstrup Sphagnum and vermiculite are mixed in a volume ratio of 9:1 as a seedling medium. The above ratio of Danish Pindstrup Sphagnum and vermiculite can achieve both good water retention and aeration properties.

[0070] (2) Disinfection of seedling medium:

[0071] Pathogens are the main cause of seedling death. Therefore, on a sunny day, mix 800 times of carbendazim, 1500 times of 50% wettable powder and 1000 times of avermectin (2% emulsion) to make a mixed disinfectant. Sprinkle it evenly on the substrate, cover it with a film and expose it to the sun for 10 days for disinfection and sterilization.

[0072] (3) Somatic embryo seedling transplanting:

[0073] The substrate was placed in a breathable non-woven seedling bag with a length of 30 cm. The roots of 10-15 cm tall tissue culture seedlings were soaked in an aqueous solution containing 0.2 wt% boletus for 0.5 h and transplanted to the culture medium. Subsequently, the culture medium was transplanted into the bag. The plant spacing of each tissue culture seedling was 5-6 cm, the row spacing was 8-10 cm, and water was applied for rooting.

[0074] (4) Cooling and moisturizing:

[0075] On the day of transplanting, place the seedling bags of transplanted tissue culture seedlings on the elevated seedbed at the bottom of the greenhouse, cover the bottom and sides of the seedbed with sand, and bury 2 / 3 of the seedling bags in the sand to keep moisture; install the spray device, sunshade net, and small film arch shed from top to bottom directly above the seedbed. When the daytime temperature is higher than 28℃, spray to keep moisture for 7 days. After the seedling slow-growing period and 15 days after transplanting, remove the small arch shed; the shading rate of the greenhouse is 80%.

[0076] (5) Photopic acclimation:

[0077] 5 days after transplanting, gradually open the film and uncover the shade net covering the lower half of the small shed to increase light; 7 days after transplanting, cover it from 10 am to 4 pm, and gradually remove the shade net after 10 days.

[0078] (6) Fertilization formula and method:

[0079] 7 days after transplanting, urea, superphosphate and potassium chloride were mixed in a mass ratio of N:P2O5:K2O=15:4:11 to obtain basal fertilizer, and the basal fertilizer was prepared into 0.3wt% water-fertilizer and sprayed into the seedling bag; 15 days after the first fertilization, the basal fertilizer was prepared into 0.5wt% water-fertilizer and applied, and then 0.5wt% water-fertilizer was applied every 15 days.

[0080] (7) Shade acclimation:

[0081] On the 10th day after transplanting, the film and the sunshade net were removed, and the seedling bags were raised in the seedbed for 50 days. After the new leaf buds were aged and stabilized (the leaves were dark green and hard), they were moved from the greenhouse to a conventional outdoor shade shed for domestication (with a shading rate of about 50%), and 1.0wt% water fertilizer was applied every 15 days; the preparation method of the water fertilizer in this step included: urea, superphosphate, and potassium chloride were mixed according to a mass ratio of N:P2O5:K2O=18:7:12 to obtain a base fertilizer, and the base fertilizer was prepared into a 1.0wt% water fertilizer.

[0082] (8) Leave the field:

[0083] When the second new leaf is aging, open the sunshade net and carry out full-sun acclimatization and hardening. When the third new leaf is pulled out and the aging is stable, it can be planted in the nursery. The whole seedling raising cycle is 5 months.

[0084] Example 3

[0085] (1) Prepare suitable seedling medium:

[0086] Danish Pindstrup Sphagnum and vermiculite are mixed in a volume ratio of 5:1 as a seedling medium. The above ratio of Danish Pindstrup Sphagnum and vermiculite can achieve both good water retention and aeration properties.

[0087] (2) Disinfection of seedling medium:

[0088] Pathogens are the main cause of seedling death. Therefore, on a sunny day, mix 800 times of carbendazim, 1500 times of 50% wettable powder and 1000 times of avermectin (2% emulsion) to make a mixed disinfectant. Sprinkle it evenly on the substrate, cover it with a film and expose it to the sun for 10 days for disinfection and sterilization.

[0089] (3) Somatic embryo seedling transplanting:

[0090] The substrate was placed in a breathable non-woven seedling bag with a length of 30 cm. The roots of 10-15 cm tall tissue culture seedlings were soaked in an aqueous solution containing 0.3 wt% boletus for 0.5 h and transplanted to the culture medium. Subsequently, the culture medium was transplanted to the bag. The plant spacing of each tissue culture seedling was 5-6 cm, the row spacing was 8-10 cm, and rooting water was poured.

[0091] (4) Cooling and moisturizing:

[0092] On the day of transplanting, place the seedling bags of transplanted tissue culture seedlings on the elevated seedbed at the bottom of the greenhouse, cover the bottom and sides of the seedbed with sand, and bury 2 / 3 of the seedling bags in the sand to keep moisture; install the spray device, sunshade net, and small film arch shed from top to bottom directly above the seedbed. When the daytime temperature is higher than 28℃, spray to keep moisture for 7 days. After the seedling slow-growing period and 15 days after transplanting, remove the small arch shed; the shading rate of the greenhouse is 80%.

[0093] (5) Photopic acclimation:

[0094] 5 days after transplanting, gradually open the film and uncover the shade net covering the lower half of the small shed to increase light; 7 days after transplanting, cover it from 10 am to 4 pm, and gradually remove the shade net after 10 days.

[0095] (6) Fertilization formula and method:

[0096] 7 days after transplanting, urea, superphosphate and potassium chloride were mixed in a mass ratio of N:P2O5:K2O=18:4:11 to obtain basal fertilizer, and the basal fertilizer was prepared into a 0.3wt% water-fertilizer and sprayed into the seedling bag; 15 days after the first fertilization, the basal fertilizer was prepared into a 0.5wt% water-fertilizer and applied, and then 0.5wt% water-fertilizer was applied every 15 days.

[0097] (7) Shade acclimation:

[0098] On the 10th day after transplanting, the film and the sunshade net were removed, and the seedling bags were raised in the seedbed for 50 days. After the new leaf buds were aged and stabilized (the leaves were dark green and hard), they were moved from the greenhouse to a conventional outdoor shade shed for domestication (with a shading rate of about 50%), and 1.0wt% water fertilizer was applied every 15 days; the preparation method of the water fertilizer in this step included: urea, superphosphate, and potassium chloride were mixed according to a mass ratio of N:P2O5:K2O=10:7:12 to obtain a base fertilizer, and the base fertilizer was prepared into a 1.0wt% water fertilizer.

[0099] (8) Leave the field:

[0100] When the second new leaf is aging, open the sunshade net and carry out full-sun acclimatization and hardening. When the third new leaf is pulled out and the aging is stable, it can be planted in the nursery. The whole seedling raising cycle is 5 months.

[0101] Test example

[0102] In the test field of each embodiment and comparative example, 10 seedlings were randomly selected, and the distance between the leaf canopies and the plant height were measured with a tape measure, the stem diameter (5 cm above the root) was measured with a digital display vernier caliper, and the number of leaf canopies was counted; the SPAD value of the leaves was measured with a handheld SPAD-502 chlorophyll meter; and the emergence rate (the percentage of the number of emerging plants in each test field to the total number of cultivated plants) was calculated. The test results are shown in Table 1.

[0103] Table 1

[0104]

[0105]

[0106] It can be seen from Table 1 that, compared with the comparative example, the method in the embodiment of the present invention can promote the healthy growth of rubber tree tissue culture seedlings and improve the greenness of the leaves.

[0107] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cultivating rubber tree tissue culture seedlings by one-step seedling cultivation, characterized in that, The following steps are involved: (1) mixing the roots of rubber tree tissue culture seedlings with the arabinose / IAA composite solution; (2) planting the rubber tree tissue culture seedlings obtained in step (1) in a greenhouse; (3) after planting, fertilizing the rubber tree tissue culture seedlings; After planting, a film shed and a sunshade net are set up above the rubber tree tissue culture seedlings; After 5 days of planting, cover half of the shade net; after 7 days of planting, cover half of the shade net from 10 am to 4 pm, and remove the shade net during the remaining time; after 10 days of planting, remove the shade net and film arch shed.

2. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 1, is characterized in that, The rubber tree tissue culture seedlings are planted in a seedling medium; the seedling medium is made by mixing Danish peat soil and vermiculite.

3. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 1, is characterized in that, In the arabinose / IAA composite solution, the concentration of arabinose is 5 to 10 wt %; In the arabinose / IAA composite solution, the concentration of IAA is 100-200 mg / L.

4. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 1, is characterized in that, The fertilization is carried out 7 to 40 days after the rubber tree tissue culture seedlings are planted; The fertilization includes 6-8 times of fertilization; the first fertilization is on the 7th to 9th day after planting the rubber tree tissue culture seedlings, the second fertilization is on the 21st to 24th day after planting the rubber tree tissue culture seedlings, and the third fertilization is on the 36th to 40th day after planting the rubber tree tissue culture seedlings; and subsequently, fertilization is performed every 15 to 20 days.

5. the rubber tree tissue culture seedling cultivation method of the one-step seedling raising according to claim 1 or 4, is characterized in that, The fertilization specifically comprises: mixing nitrogen fertilizer, phosphorus fertilizer, potash fertilizer and water to obtain a mixed solution, and fertilizing the rubber tree tissue culture seedlings with the mixed solution; In the mixed solution, the total content of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer is 0.3-0.5wt%.

6. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 5, is characterized in that, The nitrogen fertilizer includes urea; the phosphorus fertilizer includes superphosphate; and the potash fertilizer includes potassium chloride.

7. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 1, is characterized in that, The following steps are also included: 50 to 120 days after the rubber tree tissue culture seedlings are planted, topdressing is performed on the rubber tree tissue culture seedlings; the topdressing specifically includes: 50 days after the rubber tree tissue culture seedlings are planted, the first topdressing is performed, and then topdressing is performed once every 15 days.

8. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 7, is characterized in that, The topdressing specifically comprises: mixing nitrogen fertilizer, phosphorus fertilizer, potash fertilizer and water to obtain a water-fertilizer solution, and topdressing the rubber tree tissue culture seedlings with the water-fertilizer solution; In the water fertilizer solution, the total content of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer is 0.5-1.0wt%.

9. the rubber tree tissue culture seedling cultivation method of one-step seedling raising according to claim 8, is characterized in that, The nitrogen fertilizer includes urea; the phosphorus fertilizer includes superphosphate; and the potash fertilizer includes potassium chloride.

10. The rubber tree tissue culture seedling cultivation method of one-step seedling cultivation according to claim 1, wherein After 50 days of planting, the rubber tree tissue culture seedlings are moved from the greenhouse to a shade shed; the shading rate of the shade shed is 70%.

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