Antiviral traditional Chinese medicine composition for animals and application thereof

Abstract

The invention provides an antiviral traditional Chinese medicine composition for animals and application thereof, and relates to the technical field of pharmacy of traditional Chinese medicines for animals, the traditional Chinese medicine composition comprises 10-30 parts of commelina communis, 10-30 parts of scutellaria baicalensis, 10-30 parts of ligusticum wallichii, 10-30 parts of verbena, 10-30 parts of radix bupleuri, 10-30 parts of wild buckwheat, 10-30 parts of caulis clematidis armandii, 10-30 parts of rhizoma alismatis and 10-30 parts of codonopsis pilosula. The traditional Chinese medicine composition disclosed by the invention has a remarkable treatment effect on various animal viral diseases such as influenza, infectious bursal disease, porcine circovirus disease, infectious bronchitis, duck plague, rotavirus infection, transmissible gastroenteritis, bovine viral diarrhea, rabbit hemorrhagic disease, feline reovirus infection, feline plague, mink viral enteritis and the like; the animal death rate is reduced, the organism immunity is enhanced, and the growth performance is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine pharmaceutical preparation for animals, and in particular to an antiviral Chinese medicine composition for animals and its application Background Art

[0002] In recent years, the incidence of animal viral diseases in animal breeding is high, which is a major category of diseases that has caused huge losses to my country's breeding animal industry, such as Newcastle disease, influenza, blue ear disease, circovirus disease, adenovirus infection, African swine fever, blue ear disease, vesicular disease, epidemic diarrhea, rotavirus infection, rabbit viral hemorrhagic disease, feline reovirus infection, feline plague, mink viral enteritis and many other viral diseases. In breeding production, many animal viral diseases have not yet had specific drugs. At present, the prevention and control of viral diseases mainly include vaccination and Chinese herbal medicine. Vaccination and immunization have good effects in the early stage, but it requires multiple vaccinations, which is troublesome to operate, costly, and easy to cause animal stress response. Some viral diseases have not yet had mature vaccines available. At the same time, viruses are easy to mutate, and existing vaccines have problems such as incomplete protection and general prevention effect. Due to its safety, low toxicity, green, and no residue, many researchers use Chinese herbal medicine in the prevention and treatment of animal viral diseases. Chinese herbal medicine has significant effects in the prevention of animal viral diseases and the early and mid-term therapeutic efficacy. Traditional Chinese medicine theory emphasizes the relationship between viruses and the body, and Chinese medicine has the comprehensive effects of inhibiting viral replication, reducing virus-induced damage to the body, regulating immune function, and enhancing the body's ability to resist viruses. Therefore, it has become a hot topic of concern for scholars at home and abroad in recent years. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] The invention provides an antiviral Chinese medicinal composition for animals and application thereof. The Chinese medicinal composition has significant antiviral and immune enhancement effects on animal viral diseases.

[0005] (II) Technical solution

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] The invention provides an antiviral Chinese medicinal composition for animals. The Chinese medicinal composition is composed of the following raw materials in parts by weight: 10-30 parts of Commelina communis, 10-30 parts of Scutellaria baicalensis, 10-30 parts of Ligusticum chuanxiong, 10-30 parts of Verbena officinalis, 10-30 parts of Bupleurum chinense, 10-30 parts of Fagopyrum scabra, 10-30 parts of Akebia trifoliata, 10-30 parts of Orientalis orientalis and 10-30 parts of Codonopsis pilosula.

[0008] Preferably, the Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of Commelina, 15-25 parts of Scutellaria, 15-25 parts of Ligusticum chuanxiong, 15-25 parts of Verbena, 15-25 parts of Bupleurum, 15-25 parts of Fagopyrum scabra, 15-25 parts of Akebia, 15-25 parts of Alisma, and 15-25 parts of Codonopsis pilosula.

[0009] Preferably, the Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Commelina, 16 parts of Scutellaria, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena, 18 parts of Bupleurum, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Alisma orientalis, and 16 parts of Codonopsis pilosula.

[0010] The Chinese medicine composition provided by the present invention is used to prepare medicines for preventing and treating animal viral diseases. The Chinese medicine composition can be made into oral preparations acceptable to animals, including but not limited to: granules, powders, extracts, pills, lyophilized powders, and oral liquids.

[0011] Commelina: sweet, light in taste, cold in nature, enters the lung, stomach, and small intestine meridians; has the effects of clearing away heat and purging fire, detoxifying, promoting diuresis and reducing swelling; used for colds and fever, thirst due to febrile diseases, sore throat, edema and oliguria, painful and astringent stranguria due to heat, carbuncles and furuncles.

[0012] Scutellaria baicalensis: bitter in taste, cold in nature, enters the spleen and lung meridians; it contains baicalin, baicalein, wogonin, baicalein I, II, norwogonin, volatile oil, phenylethanol glycosides, etc., which can clear away heat and dampness, purge fire and detoxify, stop bleeding, stabilize pregnancy, lower blood pressure, kill bacteria, kill viruses, kill fungi, etc. Baicalin and baicalein contained in Scutellaria baicalensis have good antioxidant function. Human medicine uses Scutellaria baicalensis to treat ischemic stroke (cerebral infarction) and Parkinson's disease, and veterinary medicine uses it to treat foot-and-mouth disease, blister disease, clear lung heat, and eliminate respiratory tract infections.

[0013] Ligusticum chuanxiong: pungent, warm; enters the liver and gallbladder meridians; promotes qi and relieves depression, dispels wind, dryness and dampness, promotes blood circulation and relieves pain; used for wind-cold headache and dizziness, flank pain and abdominal pain, cold arthralgia and muscle spasms, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncle, ulcer, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to lumps, stabbing pain in the chest and flanks, swelling and pain due to falls, headache, and rheumatic pain.

[0014] Verbena: bitter, cool. Enters the liver and spleen meridians. Clears away heat and detoxifies, promotes blood circulation and dissipates blood stasis, promotes diuresis and reduces swelling. Treats exogenous fever, damp-heat jaundice, edema, dysentery, malaria, diphtheria, throat paralysis, gonorrhea, amenorrhea, masses, carbuncle, ulcer, and tooth ulcer. "Ben Cao Jing Shu": Verbena is a blood-cooling and blood-breaking medicine. The lower part of the abscess is caused by extreme heat in the blood, damp heat, so the blood is dirty and turbid, and there are worms. The blood is cooled and heat is resolved, and the dirty blood is broken and circulated, and everything is cured. Chen Cangqi said that it can break blood and kill worms, which is also the meaning.

[0015] Bupleurum: bitter and pungent in taste, slightly cold in nature, enters the liver and gallbladder meridians; has the effects of reconciling the exterior and interior, soothing the liver and relieving depression, raising yang and lifting sunkenness, reducing fever and stopping malaria; used for colds and fever, alternating chills and heat, chest and flank pain, irregular menstruation, uterine prolapse, rectal prolapse and other symptoms; it contains volatile oils, saponins, flavonoids and other active ingredients; modern pharmacology has proved that Bupleurum has anti-inflammatory, anti-tumor, antibacterial, antiviral, antipyretic, sedative, analgesic, antitussive and other effects, and has a regulatory effect on the liver, gastrointestinal tract, immune function, blood lipids, etc.

[0016] Golden buckwheat: sour, bitter, cold in nature. Enters the lung, stomach, and liver meridians; has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and removing rheumatism; mainly treats lung abscess; lung heat cough and asthma; sore throat; dysentery; rheumatic arthralgia; traumatic injury; carbuncle, sore, snake and insect bites.

[0017] Chuanmutong: bitter in taste, cold in nature, enters the heart, small intestine, and bladder meridians; has the effects of diuresis, relieving stranguria, clearing the heart and eliminating restlessness, and relieving dysmenorrhea and lactation; used for stranguria, edema, restlessness and red urine, mouth and tongue ulcers, amenorrhea, oligolactosis, damp-heat arthralgia and other symptoms.

[0018] Alisma: It is cold in nature, sweet and light in taste, and enters the kidney and bladder meridians. It has the effects of promoting diuresis and eliminating dampness, purging heat, clearing turbidity and regulating lipids. It can be used for symptoms such as difficulty urinating, bloating, diarrhea and oliguria. It mainly contains chemical components such as triterpenes and sesquiterpenes, and has pharmacological effects such as enhancing immunity and anti-inflammation.

[0019] Codonopsis: sweet in taste, neutral in nature; enters the spleen and lung meridians; has the effects of tonifying the middle and replenishing Qi, strengthening the spleen and lungs, nourishing blood and producing body fluids; it contains sugars, glycosides, alkaloids, amino acids, trace elements and other ingredients, which can significantly enhance the immunity of animals, improve the production performance of animals, enhance the anti-stress ability of animals, have bactericidal ability, and at the same time have a high nutritional effect.

[0020] The main cause of animal viral diseases is heat toxicity and dampness, and the main disease site is the lungs, which can also affect other organs. The basic pathogenesis is heat toxicity blocking the lungs, lung qi stagnation, phlegm dampness and blood stasis, and qi and yin deficiency. In the prescription, Commelina communis is cold in nature and has the function of clearing heat and purging fire, so it is the main medicine. It is used together with Verbena, Scutellaria, Bupleurum, Fagopyrum, Alisma, and Chuanketong to clear heat and detoxify, promote diuresis and relieve heat, so as to strengthen the function of evacuating wind and heat; in the late stage of the disease, if the qi does not transform into body fluid, the body fluid will be refined into phlegm, and if the qi does not move blood, it will be stagnant, phlegm and blood stasis will damage the collaterals, and the evil heat toxicity will consume qi and damage yin. Qi and yin deficiency runs through the entire course of the disease, and Chuanxiong is used to promote blood circulation and dissipate blood stasis, and Codonopsis pilosula is used to strengthen the spleen and lungs, nourish blood and produce body fluid, with the purpose of strengthening the body and eliminating evil.

[0021] (III) Beneficial effects

[0022] The Chinese medicine composition of the present invention has a significant therapeutic effect on a variety of animal viral diseases such as influenza, infectious bursal disease, porcine circovirus disease, infectious bronchitis, duck plague, rotavirus infection, infectious gastroenteritis, bovine viral diarrhea, feline reovirus infection, mink viral enteritis, etc., reduces animal mortality, enhances body immunity, improves growth performance, etc. The Chinese medicine composition of the present invention can improve the body's immune organ index, improve the body's immunity, improve the body's disease resistance, has a significant antiviral effect, and achieves simultaneous treatment of the symptoms and the root cause, and prevention and treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The bursa index (A), thymus index (B), spleen index (C) of the experimental chickens in each group; compared with the blank control group, *p<0.05, **p<0.01. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] An antiviral Chinese medicine composition for animals, the Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Commelina communis, 16 parts of Scutellaria baicalensis, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena, 18 parts of Bupleurum, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Alisma orientalis, and 16 parts of Codonopsis pilosula.

[0027] The Chinese medicine composition can be made into an oral preparation acceptable to animals, including but not limited to: granules, powders, extracts, pills, freeze-dried powders, and oral liquids.

[0028] Example 2

[0029] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts of Commelina, 10 parts of Scutellaria, 10 parts of Ligusticum chuanxiong, 10 parts of Verbena, 10 parts of Bupleurum, 10 parts of Fagopyrum scabra, 10 parts of Caulis Akebiae, 10 parts of Alisma orientalis, and 10 parts of Codonopsis pilosula.

[0030] Example 3

[0031] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of Commelina, 30 parts of Scutellaria, 30 parts of Ligusticum chuanxiong, 30 parts of Verbena, 30 parts of Bupleurum, 30 parts of Fagopyrum scabra, 30 parts of Akebia trifoliata, 30 parts of Alisma orientalis, and 30 parts of Codonopsis pilosula.

[0032] Example 4

[0033] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts of Commelina, 14 parts of Scutellaria, 12 parts of Ligusticum chuanxiong, 12 parts of Verbena, 12 parts of Bupleurum, 11 parts of Fagopyrum scabra, 10 parts of Caulis Akebia, 11 parts of Alisma orientalis, and 12 parts of Codonopsis pilosula.

[0034] Example 5

[0035] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 26 parts of Commelina, 22 parts of Scutellaria, 18 parts of Ligusticum chuanxiong, 20 parts of Verbena, 26 parts of Bupleurum, 14 parts of Fagopyrum scabra, 14 parts of Caulis Akebiae, 14 parts of Alisma orientalis, and 18 parts of Codonopsis pilosula.

[0036] Example 6

[0037] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Commelina, 20 parts of Scutellaria, 18 parts of Ligusticum chuanxiong, 17 parts of Verbena, 16 parts of Bupleurum, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 18 parts of Alisma orientalis, and 20 parts of Codonopsis pilosula.

[0038] Example 7

[0039] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of Commelina, 18 parts of Scutellaria, 15 parts of Ligusticum chuanxiong, 18 parts of Verbena, 20 parts of Bupleurum, 15 parts of Fagopyrum scabra, 15 parts of Caulis Akebiae, 15 parts of Alisma orientalis, and 18 parts of Codonopsis pilosula.

[0040] Example 8

[0041] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of Commelina, 23 parts of Scutellaria, 18 parts of Ligusticum chuanxiong, 20 parts of Verbena, 22 parts of Bupleurum, 17 parts of Fagopyrum scabra, 17 parts of Caulis Akebiae, 16 parts of Alisma orientalis, and 19 parts of Codonopsis pilosula.

[0042] Example 9

[0043] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Commelina, 20 parts of Scutellaria, 26 parts of Ligusticum chuanxiong, 20 parts of Verbena, 24 parts of Bupleurum, 14 parts of Fagopyrum scabra, 19 parts of Caulis Akebia, 17 parts of Alisma orientalis, and 16 parts of Codonopsis pilosula.

[0044] Example 10

[0045] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts of Commelina, 13 parts of Scutellaria, 11 parts of Ligusticum chuanxiong, 11 parts of Verbena, 10 parts of Bupleurum, 12 parts of Fagopyrum scabra, 11 parts of Caulis Akebiae, 12 parts of Alisma orientalis, and 14 parts of Codonopsis pilosula.

[0046] Test Example 1

[0047] 2,100 laying hens from a laying hen farmer in Zhoukou City were sick. The chickens began to shake their noses, and the condition did not improve after 3 days of drinking water with tylosin. The sick chickens stood still, their combs turned purple, and their feces were yellow-green. The egg production rate dropped below 70%. Autopsy showed congestion of the laryngeal and tracheal mucosa, no papillary bleeding in the glandular stomach, bleeding spots in the cecum tonsils, congestion of follicles, and edema of the oviduct contents. Laboratory tests diagnosed it as mild avian influenza infected with the H9 subtype.

[0048] Trial Drugs:

[0049] Drug 1: 22 parts of Commelina, 16 parts of Scutellaria, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena, 18 parts of Bupleurum, 16 parts of Radix Aconiti Lateralis, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Rhizoma Alismatis, and 16 parts of Codonopsis pilosula.

[0050] Drug 2: 16 parts of Scutellaria baicalensis, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena officinalis, 16 parts of Radix Aconiti Lateralis Preparata, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Rhizoma Alismatis, and 16 parts of Codonopsis pilosula.

[0051] Drug 3: 22 parts of Commelina, 16 parts of Ligusticum chuanxiong, 18 parts of Bupleurum, 16 parts of Radix Aconiti Lateralis Preparata, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Rhizoma Alismatis, and 16 parts of Codonopsis pilosula.

[0052] Medicine 4: 22 parts of Commelina, 16 parts of Scutellaria, 20 parts of Verbena, 18 parts of Bupleurum, 16 parts of Radix Aconiti Lateralis, 16 parts of Fagopyrum scabra, and 16 parts of Codonopsis pilosula.

[0053] Drug 5: 22 parts of Commelina, 16 parts of Scutellaria, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena, 18 parts of Bupleurum, 18 parts of Caulis Akebiae, and 16 parts of Alisma.

[0054] Drug 6: Jingfang Baidu San, prepared according to traditional formula.

[0055] According to the weight proportions of the above-mentioned formulas, the raw materials are weighed respectively, washed, dried, and ultrafinely ground to obtain Chinese medicine powders of medicines 1-6.

[0056] The above-mentioned diseased chickens were divided into 7 groups by digital random method, 300 in each group, respectively blank group and observation groups 1-6. The blank group was not given any medicine, and the observation groups 1-6 were fed with medicines 1-6 respectively, with a dose of 2kg mixed with 300kg feed. Each group had free access to food and diet, and the other feeding conditions remained the same. Each group was given medicine for 7 consecutive days, and continued to be observed for 3 days after stopping the medicine, and the changes and health status of the chickens were observed every day.

[0057] Efficacy criteria:

[0058] Cure: normal body temperature, normal feed intake, normal spirit, external symptoms such as combs return to normal, and egg production returns to more than 90%;

[0059] Effective: body temperature is normal, feed intake increases, spirits are basically restored, external symptoms such as combs are significantly improved, and egg production recovers to more than 80%;

[0060] Ineffective: The body temperature remains high, the feed intake decreases, the chickens become depressed, external symptoms such as the comb do not improve, and the egg production continues to drop below 70%.

[0061] The therapeutic effects of each group of sick chickens are shown in Table 1.

[0062] Table 1 Treatment effect of sick chickens in each group

[0063]

[0064]

[0065] As shown in Table 1, the mortality rate of the sick chickens in the blank group was as high as 82.33% without any drug treatment. Compared with the blank group, after the observation groups 1-6 were treated with different drugs, the total effective rate of the sick chickens in each group was above 90%, indicating that drugs 1-6 had significant therapeutic effects on avian influenza and could significantly reduce the mortality rate of sick chickens. The death of observation group 1 stopped on the second day of administration, the death of observation groups 2 and 3 stopped on the fifth day of administration, the death of observation groups 4 and 5 stopped on the fourth day of administration, and the death of observation group 6 stopped on the sixth day of administration; the mortality rate of observation group 1 was only 0.67%, which was significantly lower than that of the other observation groups. Although the total effective rate of the treatment of avian influenza-sick chickens in each observation group was above 90%, the cure rate of observation group 1 was 82.00%, and the cure rates of the other groups were between 45-65%, and the cure rate of observation group 1 was significantly higher than that of the other observation groups. The above results show that the Chinese medicine composition of the present invention has a more significant therapeutic effect on avian influenza than drugs 2-5 and Jingfang Baidu Powder, has a higher cure rate, significantly reduces the symptoms of diseased laying hens, improves mental state, increases feed intake, increases egg production rate, and reduces mortality. The Chinese medicine composition of the present invention has synergistic effects among the drugs, and each component is indispensable.

[0066] Test Example 2

[0067] 1 Experimental Drug

[0068] (1) Traditional Chinese medicine stock solution of the present invention: weigh the following raw materials according to the following weight proportions: 20 parts of Commelina, 18 parts of Scutellaria, 15 parts of Ligusticum chuanxiong, 18 parts of Verbena, 20 parts of Bupleurum, 15 parts of Radix Aconiti Lateralis, 15 parts of Fagopyrum scabra, 15 parts of Caulis Akebiae, 15 parts of Rhizoma Alismatis, 15 parts of Codonopsis pilosula, wash, dry, grind into powder, add 10 times (m / V) amount of distilled water, soak for 30 min, decoct for 1 h, obtain filtrate, add 8 times amount of distilled water again, decoct for 1 h, combine the filtrates, concentrate to a crude drug content of 1 g / mL, and store at 4°C for later use.

[0069] (2) Isatis Root Granules

[0070] (3) Astragalus polysaccharide oral solution

[0071] 2 Experimental animals

[0072] A broiler farm in Zhoukou City found that healthy chickens suddenly became ill, and the disease was serious. The chickens showed symptoms such as listlessness, head shrinkage, closed eyes, lethargy, loss of appetite, ruffled feathers, increased water intake, and rice soup-like feces. After autopsy, the naked eye observed that there were brush-like bleeding in the body and legs, the bursa of Fabricius was enlarged or not enlarged, and the bursa of Fabricius was congested. Some chickens had severe bleeding, with a purple grape-like appearance. Most of the cysts were yellow, and some had yellow-white jelly-like fluid; the duodenum had hemorrhagic patchy bleeding, and some of the gizzards and glandular stomachs had bleeding spots; the kidneys were enlarged or had urate deposits; and a small number of chickens died. No drug treatment was used. After clinical observation and laboratory diagnosis, it was confirmed that it was infectious bursal disease in chickens and was determined as experimental animals. 800 chickens were randomly selected from the sick chickens and randomly divided into 4 groups, with 200 chickens in each group, 30 days old broilers. The experimental chickens were all raised in standard chicken houses, raised on the net, immunized according to the routine immunization program, and free access to food and water.

[0073] 3 Experimental Grouping and Treatment

[0074] The experimental animals were randomly divided into 4 groups, each with 200 chickens, namely, blank control group, observation group, control group 1, and control group 2. The blank control group was not given any medicine and was raised normally; the observation group was given the Chinese medicine stock solution of the present invention, and 1% (1mL of Chinese medicine stock solution was diluted with 100mL of water) was given to drinking water; the control group 1 was given 3g / L of Radix Isatidis granules with drinking water; the control group 2 was given 1% Astragalus polysaccharide oral solution with drinking water (1mL of Astragalus polysaccharide oral solution was diluted with 100mL of water). The experimental period was 10 days, including 5 days of treatment and 5 days of observation after treatment.

[0075] 3 Result evaluation indicators

[0076] 3.1 Observation of efficacy

[0077] The effect of the Chinese medicine composition of the present invention on the cure rate, effective rate and mortality rate of chickens with infectious bursal disease was observed and the efficacy was determined.

[0078] (1) Cure: Clinical symptoms disappear, and mental state, appetite, stool, breathing, etc. return to normal;

[0079] (2) Effective: Chickens with improved clinical symptoms were considered effective;

[0080] (3) Ineffective: The clinical symptoms of the chickens did not improve, or even worsened. The test chickens showed typical symptoms of bursal disease and died, which was determined to be death from bursal disease virus infection.

[0081] 3.2 Immune organ index

[0082] 5 days after administration, 15 chickens were randomly selected from each group, each chicken was weighed, and the bursa of Fabricius, thymus, and spleen were collected to calculate the immune organ index, which is: immune organ weight (mg) / body weight (g)

[0083] 4. Data Statistics and Analysis

[0084] SPSS 21.0 software was used for statistical analysis, and the results were expressed as mean ± standard deviation (Mean ± SD). Graphpad Prism 8.0 software was used for data statistical analysis and drawing. One-way analysis of variance was used to compare the differences between the groups, and P < 0.05 was considered statistically significant.

[0085] 5 Results

[0086] 5.1 Clinical efficacy

[0087] From the results in Table 2, it can be seen that the Chinese medicine composition of the present invention can significantly reduce the mortality rate of infectious bursal disease in chickens, the total effective rate of the disease can reach 97.0%, and the cure rate can reach 84.55%. The therapeutic effect is significantly better than that of Isatis Root Granules and Astragalus Polysaccharide.

[0088] Table 2 Clinical efficacy results of each group

[0089] Group quantity Cure (only) Valid (only) Invalid (only) Death (only) Cure rate Total Effectiveness mortality rate Blank control group 200 — — 115 — — 63.0％ Observation Group 200 169 25 6 1 84.5％ 97.0％ 0.5％ Control group 1 200 137 39 24 8 68.5％ 88.0％ 4.0％ Control group 2 200 119 49 32 10 59.5％ 84.0％ 5.0％

[0090] 5.2 Immune organ index

[0091] The immune organ index of each group of experimental chickens is as follows Figure 1 shown. Figure 1 A is the bursal index of each group of experimental chickens. Compared with the blank control group, the bursal index of the observation group and control group 1-2 was significantly higher than that of the blank control group (P<0.05, P<0.01). Figure 1B is the thymus index of each group of experimental chickens. The thymus index of the observation group was significantly higher than that of the blank control group, and the difference was extremely significant (P<0.01); the thymus index of the control group 1 was significantly higher than that of the blank control group, and the difference was significant (P<0.05); the thymus index of the control group 2 was slightly higher than that of the blank control group, and there was no significant difference (P>0.05). Figure 1 C is the spleen index of each group of experimental chickens. The spleen index of the observation group was significantly higher than that of the blank control group, with a significant difference (P<0.05); there was no significant difference between the control group 1-2 and the blank control group (P>0.05).

[0092] 6 Conclusion

[0093] The above test results show that, compared with Radix Isatidis granules and Astragalus polysaccharide oral liquid, the Chinese medicine composition of the present invention has a higher effective rate and cure rate for the treatment of infectious bursal disease in chickens, can significantly reduce the mortality rate, and has a significant therapeutic effect; at the same time, the Chinese medicine composition of the present invention can significantly improve the immune organ index of sick chickens, improve the body's immunity, and improve the body's disease resistance.

[0094] Test Example 3

[0095] Experimental animals: A group of weaned piglets in a large-scale pig farm in Xinxiang City showed clinical symptoms such as anorexia, dyspnea, coughing, and mental depression. The serum antibody test was positive for porcine circovirus and confirmed that they were infected with porcine circovirus. 150 sick piglets were selected from the diseased building and randomly divided into 5 groups, 30 in each group, namely experimental groups 1-4 and the control group.

[0096] Experimental drugs and methods: Experimental groups 1-4 were fed with the Chinese medicine powders prepared in Examples 6-9, respectively. According to the weight proportions of each formula described in Examples 6-9, each raw material was weighed, washed, dried, and ultrafinely crushed to obtain the Chinese medicine powders of Examples 6-9. The feeding method was mixed with feed and the feeding amount was 1kg of Chinese medicine powder mixed with 500kg of special feed for piglets; the control group was gavaged with astragalus polysaccharide oral solution, and the dosage was 0.5mL / kg. The experimental period was 10 days, with 7 days of administration and 3 days of observation after stopping administration. During the experiment, the pigs were allowed to eat and drink freely, and the pens were kept clean and sanitary, and disinfected regularly. Observe and record the clinical changes and cure rate of the experimental pigs.

[0097] Efficacy evaluation criteria:

[0098] Cure: The patient is mentally normal, breathing is normal, cough disappears, water and food intake recovers, and other clinical symptoms disappear, and serum antibody test is negative;

[0099] Effective: breathing becomes normal, spirits recover, water and food intake increases, coughing decreases significantly, and other clinical symptoms improve, and serum antibody tests are negative;

[0100] Ineffective: Clinical symptoms do not improve or even worsen, leading to death, and serum antibody tests are still positive.

[0101] As shown in the results of Table 3, the Chinese medicine powders prepared in Examples 6-9 have a total effective rate of more than 90% in the treatment of piglet circovirus compared with the yellow polysaccharide oral liquid, and can achieve a total effective rate of 100%, thereby avoiding the occurrence of death.

[0102] Table 3 The therapeutic effect on porcine circovirus

[0103] Grouping quantity Heal (Head) Valid(header) void(header) Death (Head) Total Effectiveness Experimental Group 1 30 20 9 1 0 96.67％ Experimental Group 2 30 21 7 2 0 93.33％ Experimental Group 3 30 22 8 0 0 100.00％ Experimental Group 4 30 18 11 1 0 96.67％ Control group 30 12 9 9 4 70.00％

[0104] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An antiviral Chinese medicine composition for animals, It is characterized in that The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10-30 parts of Commelina communis, 10-30 parts of Scutellaria baicalensis, 10-30 parts of Ligusticum chuanxiong, 10-30 parts of Verbena officinalis, 10-30 parts of Bupleurum chinense, 10-30 parts of Fagopyrum scabra, 10-30 parts of Akebia trifoliata, 10-30 parts of Orientalis orientalis, and 10-30 parts of Codonopsis pilosula.

2. An antiviral Chinese medicine composition for animals according to claim 1, It is characterized in that The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of Commelina communis, 15-25 parts of Scutellaria baicalensis, 15-25 parts of Ligusticum chuanxiong, 15-25 parts of Verbena officinalis, 15-25 parts of Bupleurum chinense, 15-25 parts of Fagopyrum scabra, 15-25 parts of Akebia trifoliata, 15-25 parts of Orientalis orientalis, and 15-25 parts of Codonopsis pilosula.

3. The antiviral Chinese medicine composition for animals according to claim 1, It is characterized in that The Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Commelina communis, 16 parts of Scutellaria baicalensis, 16 parts of Ligusticum chuanxiong, 20 parts of Verbena, 18 parts of Bupleurum chinense, 16 parts of Fagopyrum scabra, 18 parts of Caulis Akebiae, 16 parts of Alisma orientalis, and 16 parts of Codonopsis pilosula.

4. Use of the Chinese medicine composition according to any one of claims 1 to 3 for preparing a medicament for preventing and treating animal viral diseases, It is characterized in that The Chinese medicine composition can be made into an oral preparation acceptable to animals, including but not limited to: granules, powders, extracts, pills, freeze-dried powders, and oral liquids.

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