Trichoderma liquid organic fertilizer researched and developed based on citric acid fermentation tail liquid and application of trichoderma liquid organic fertilizer

Abstract

The invention discloses a trichoderma liquid organic fertilizer researched and developed based on citric acid fermentation tail liquid and application of the trichoderma liquid organic fertilizer. The trichoderma liquid organic fertilizer disclosed by the invention comprises citric acid fermentation tail liquid, an inorganic chemical fertilizer and trichoderma spores, wherein the trichoderma spores are trichoderma harzianum NJAU 4742 spores. The trichoderma liquid organic fertilizer prepared by the invention can be applied as a water culture and watering fertilizer for plants, and is suitable for improving rhizosphere soil microflora of various plants, promoting plant growth and improving disease resistance and low-temperature stress resistance. The trichoderma liquid organic fertilizer integrates the quick-acting property of an inorganic chemical fertilizer, the long-acting property of an organic fertilizer and the synergistic property of a biological bacterial fertilizer, and is suitable for diversified modern agricultural production modes.

Description

Technical Field

[0001] The invention relates to the technical field of biological liquid organic fertilizer, and in particular to a Trichoderma liquid organic fertilizer developed based on citric acid fermentation tail liquid and application thereof. Background Art

[0003] Organic fertilizers have the advantages of improving soil structure and increasing soil organic matter content, and have a significant and long-lasting effect on improving soil and crop quality. Organic fertilizers developed using beneficial rhizosphere microorganisms can supply nutrients to crops and promote their growth and development; they can promote the massive reproduction of beneficial microorganisms, thereby forming a dominant population in the rhizosphere soil micro-ecosystem of crops, which can effectively reduce the risk of crop disease; they can also significantly improve the stress resistance of crops, enabling them to better cope with adverse stresses such as drought, salinity, insect pests, diseases, and aging. Therefore, using microbial organic fertilizers instead of chemical fertilizers has more advantages than disadvantages.

[0004] At present, my country's agricultural production methods have been partially mechanized. Planting and harvesting can basically be done by mechanical equipment, but further fertilization and watering still require human input. With the change of irrigation methods, the current agricultural model has gradually shifted to liquid fertilizers and water-fertilizer integration to improve agricultural efficiency. Liquid fertilizers can be directly absorbed by crops, effectively improving the utilization efficiency and absorption rate of fertilizers. Therefore, liquid fertilizers have more advantages than traditional solid fertilizers. However, most of the common liquid fertilizers are still based on chemical fertilizers, so it is necessary to further explore liquid, organic microbial fertilizers. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a liquid organic fertilizer of Trichoderma developed based on citric acid fermentation tail liquid and its application. The liquid organic fertilizer of Trichoderma of the present invention combines the quick-acting property of inorganic fertilizer, the long-acting property of organic fertilizer and the synergistic property of biological fertilizer, and is suitable for diversified modern agricultural production methods.

[0006] The technical solution of the present invention is as follows:

[0007] A Trichoderma liquid organic fertilizer developed based on citric acid fermentation tail liquid comprises citric acid fermentation tail liquid, inorganic fertilizer and Trichoderma spores.

[0008] Furthermore, the Trichoderma spores are Trichoderma harzianum NJAU 4742 spores.

[0009] Furthermore, the organic matter content in the citric acid fermentation tail liquid is 21.6-23.7%.

[0010] Furthermore, the inorganic fertilizer is composed of urea and potassium sulfate; the mass ratio of urea to potassium sulfate is 116:3.

[0011] Furthermore, the inorganic fertilizer is added in an amount of 13.2-25.5 g / L relative to the citric acid fermentation tail liquid.

[0012] Furthermore, the total nutrient content of the biological organic water-soluble fertilizer is 8-12%, the organic matter content is 20-22%, and the number of Trichoderma spores is 5×10 7 -5×10 8 CFU / mL, pH 5.5-6.5.

[0013] An application of the Trichoderma liquid organic fertilizer in improving the ability of plants to resist low temperature stress.

[0014] An application of the Trichoderma liquid organic fertilizer in promoting plant growth.

[0015] The invention discloses an application of the Trichoderma liquid organic fertilizer in improving plant resistance to wilt disease.

[0016] Furthermore, the plants include sunflower and cucumber.

[0017] The beneficial technical effects of the present invention are:

[0018] The present invention uses citric acid fermentation tail liquid as raw material, adds Trichoderma harzianum NJAU 4742, and supplements inorganic fertilizers to prepare Trichoderma liquid organic fertilizer, so as to realize resource utilization of citric acid fermentation tail liquid. The Trichoderma liquid organic fertilizer of the present invention overcomes the shortcomings of existing organic fertilizers, such as low nutrients, slow nutrient release, high requirements for temperature, humidity, and pH, and inability to cooperate with irrigation systems for fertilization. The Trichoderma liquid organic fertilizer of the present invention can improve the rhizosphere environment, enhance plant absorption and stress resistance. The present invention adopts Trichoderma liquid organic fertilizer to significantly reduce the incidence of crop wilt, improve the disease resistance of crops, and enhance the ability of crops to resist low temperature stress. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This chart shows the growth-promoting effects of different fertilizers on sunflower.

[0020] Figure 2 This is a graph showing the disease resistance effects of different fertilizers on cucumber wilt.

[0021] Figure 3 This is a graph showing the effects of different fertilizers on cucumber's resistance to low temperature stress. DETAILED DESCRIPTION

[0022] The present invention is described in detail below in conjunction with the accompanying drawings and embodiments.

[0023] The Trichoderma harzianum NJAU 4742 used in the following examples is the Trichoderma harzianum NJAU 4742 disclosed in Chinese invention patent CN202210152393.2, which is classified and named Trichoderma guizhouense and deposited in the General Microbiological Center of China National Microbiological Culture Collection Committee on April 11, 2016, with a deposit number of CGMCC No.12166.

[0024] The sunflowers used in the following examples were purchased from Jiangsu Academy of Agricultural Sciences, and the variety was Jinli No. 18.

[0025] The cucumbers used in the following examples were purchased from Jiangsu Academy of Agricultural Sciences, and the variety was Lufeng cucumber.

[0026] The citric acid fermentation tail liquid in the following examples is produced in the citric acid production process and is obtained from Jiangsu Guoxin Xielian Biotechnology (Group) Co., Ltd.

[0027] In the following embodiments of the present invention, the organic matter content in the citric acid fermentation tail liquid is 21.6-23.7%, preferably 23%, the pH is 4, the total nitrogen is 1.7%, the total phosphorus is 3.7%, and the total potassium is 1.9%.

[0028] The experimental methods in the following examples are all conventional methods unless otherwise specified.

[0029] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0030] The preparation method of the Trichoderma harzianum NJAU 4742 spore liquid in the following examples is as follows:

[0031] 2.5 μL of NJAU 4742 spore liquid in a glycerol tube stored at -80°C was inoculated into PDA solid medium. After activation at 37°C for 48 h, bacterial blocks of the same size were obtained from the edge of the germinated colony using a cork borer and then inoculated into PDA solid medium respectively. After culturing in the dark at 28°C for 7 days, 10 mL of sterile water was added to the medium. The plate was scraped back and forth with a sterile spreading stick to prepare a spore suspension. The mycelium was filtered through 8 layers of sterile gauze to obtain the spore liquid.

[0032] The configuration method of the PDA solid culture medium used is as follows:

[0033] PDA solid culture medium: 200 g potato extract, 20 g glucose, 15 g agar, 1000 mL deionized water, 115 °C, high pressure steam sterilization for 30 min.

[0034] PDB liquid culture medium: 200 g potato extract, 20 g glucose, 1000 mL deionized water, 115 °C, high pressure steam sterilization for 30 min.

[0035] Example 1

[0036] A Trichoderma liquid organic fertilizer developed based on citric acid fermentation tail liquid, and its preparation method is as follows:

[0037] 4.64 g of urea and 0.12 g of potassium sulfate were added to 360 mL of citric acid fermentation tail liquid (the citric acid fermentation tail liquid contained 23% organic matter, pH 4, 6% total nutrients (N+P2O5+K2O), 0.6% amino acids, 1.7% N, 3.7% P, and 1.9% K) and mixed well. The pH was adjusted to 6.0, and then Trichoderma harzianum NJAU 4742 spore solution was added to make the spore concentration 5.0×10 8 CFU / mL, and after stirring evenly, Trichoderma liquid organic fertilizer was obtained.

[0038] Comparative Example 1

[0039] A fertilizer, the preparation method of which is as follows:

[0040] 13.58 g of urea, 24.95 g of diammonium phosphate and 15.32 g of potassium sulfate were mixed uniformly to obtain a fertilizer.

[0041] Comparative Example 2

[0042] An organic fertilizer, the preparation method of which is as follows:

[0043] Take 4.64 g of urea and 0.12 g of potassium sulfate, add them into 360 mL of citric acid fermentation tail liquid and mix well to obtain organic fertilizer.

[0044] Test Case

[0045] (1) The growth-promoting effect of Trichoderma liquid organic fertilizer on sunflower

[0046] The growth-promoting effect of Trichoderma liquid organic fertilizer on sunflower plants was verified through greenhouse potted experiments.

[0047] Disinfection and germination of plant seeds: After sunflower seeds were disinfected by soaking in 10% sodium hypochlorite for 15 minutes, they were then soaked in 50°C sterile water for 2 hours, and finally rinsed with clean water. The seeds were evenly spread on moist filter paper and left to germinate in the dark, and then sown in pots containing seedling medium (purchased from Xiangzheng Agricultural Science) for 5 days, and then the seedlings with good and consistent growth were selected for transplanting. A total of 4 groups (6 replicates, i.e. 6 pots, were set up in each group) were set up for the potted plant experiment, as follows:

[0048] Control group T1: administration of clean water;

[0049] Fertilizer group T2: using the fertilizer prepared in Comparative Example 1;

[0050] Organic fertilizer group T3: applying the organic fertilizer prepared in Comparative Example 2;

[0051] Single bacteria group T4: spore concentration was 5.0×10 8 CFU / mL of Trichoderma harzianum NJAU 4742 spore solution;

[0052] Trichoderma liquid organic fertilizer group T5: using the Trichoderma liquid organic fertilizer prepared in Example 1;

[0053] Three crops were planted in each pot, and each pot was filled with 3 kg of soil. The total amount of fertilizer applied to each group was 2% of the total mass of the soil. Fertilizer was applied every 6 days and applied to the soil 5 times on average. Each time the fertilizer was diluted at a ratio of 1:60 and then applied to the soil at the root of the crop. During the pot experiment, the greenhouse planting conditions were maintained as follows: the photoperiod was 16 h per day (120 μmol photons m -2 ·s -1 ), temperature 26 / 23℃ (day / night). Water once every other day, 500mL of water per pot each time, no watering on the day of fertilization. The planting cycle is 40 days. After the treatment, the plants are harvested and tested for indicators such as plant height, stem diameter, and plant dry and fresh weight. The results are as follows Figure 1 And as shown in Table 1.

[0054] Table 1 Effects of different fertilizer treatments on the biological morphology of sunflower

[0055]

[0056]

[0057] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level

[0058] from Figure 1As can be seen from the results in Table 1, the plant heights of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 33.20 cm, 47.73 cm, 51.90 cm, 52.83 cm and 56.70 cm, respectively. The plant heights of the chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were significantly increased compared with the control group, among which the plant height of the Trichoderma liquid organic fertilizer group was 120.01% higher than that of the control group. The stem diameters of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 3.73mm, 4.83mm, 5.40mm, 5.83mm and 6.03mm, respectively. The stem diameters of the organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were significantly higher than those of the control group and chemical fertilizer group. The root lengths of the Trichoderma liquid organic fertilizer group were significantly higher than those of the control group, chemical fertilizer group, organic fertilizer group and single bacteria group. Among them, the stem diameter of the Trichoderma liquid organic fertilizer group was 61.66% higher than that of the control group and 24.84% higher than that of the chemical fertilizer group. The root lengths of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 9.20cm, 10.87cm, 12.77cm, 13.53cm and 14.57cm, respectively. The root lengths of the Trichoderma liquid organic fertilizer group were 58.37% higher than those of the control group, 34.04% higher than those of the chemical fertilizer group, 14.10% higher than those of the organic fertilizer group and 7.7% higher than those of the single bacteria group. This shows that the application of Trichoderma liquid organic fertilizer can significantly increase the plant height, stem thickness and root length of crops.

[0059] The aboveground fresh weight of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 4.63g, 9.68g, 16.02g, 16.28g and 17.27g respectively; the dry weight was 1.90g, 2.13g, 3.50g, 4.80g and 4.69g respectively; the aboveground fresh weight of the organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group was significantly higher than that of the control group and chemical fertilizer group; the aboveground dry weight of the single bacteria group and Trichoderma liquid organic fertilizer group was significantly higher than that of the control group, chemical fertilizer group and organic fertilizer group. Among them, the aboveground fresh weight of the Trichoderma liquid organic fertilizer group was 273.00% higher than that of the control group, and 78.41% higher than that of the chemical fertilizer group; the aboveground dry weight of the Trichoderma liquid organic fertilizer group was 146.84% higher than that of the control group, 112.21% higher than that of the chemical fertilizer group, and 34% higher than that of the organic fertilizer group. The fresh weight of the underground part of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 0.49g, 1.43g, 3.54g, 3.80g and 4.51g respectively; the dry weight of the underground part was 0.14g, 0.26g, 0.54g, 0.80g and 1.12g respectively. The dry and fresh weight of the underground part of the Trichoderma liquid organic fertilizer group was significantly higher than that of the control group, chemical fertilizer group, organic fertilizer group and single bacteria group. Among them, the fresh weight of the underground part of the Trichoderma liquid organic fertilizer group was 820.41% higher than that of the control group, 215.38% higher than that of the chemical fertilizer group, 27.40% higher than that of the organic fertilizer group, and 18.68% higher than that of the single bacteria group. The dry weight of the underground part of the Trichoderma liquid organic fertilizer group was 700% higher than that of the control group, 330.77% higher than that of the chemical fertilizer group, 107.41% higher than that of the organic fertilizer group, and 40.00% higher than that of the single bacteria group. This indicated that the application of Trichoderma liquid organic fertilizer significantly increased the aboveground and underground dry and fresh weights of crops.

[0060] The above results show that the Trichoderma liquid organic fertilizer prepared by the present invention can significantly improve the biological traits of crops.

[0061] (2) Disease resistance effect of Trichoderma liquid organic fertilizer

[0062] The disease resistance effect of Trichoderma liquid organic fertilizer on cucumber plants was verified through greenhouse potted experiments.

[0063] a. Preparation method of Fusarium oxysporum spore solution:

[0064] 2.5 μL of spores of Fusarium oxysporum in a glycerol tube stored at -80°C was inoculated into PDA solid medium. After activation at 28°C for 72 h, bacterial blocks of the same size were obtained from the edge of the germinated colonies using a cork puncher. These blocks were then inoculated into PDB liquid medium. After culturing at 25°C for 5 days, the mycelium was filtered with sterile gauze, and the concentration of the spores of Fusarium oxysporum was determined using a hemocytometer. The concentration was 5×10 6CFU / mL of Fusarium oxysporum spore liquid.

[0065] b. Disinfection and germination of plant seeds: After the cucumber seeds are disinfected by soaking in 10% sodium hypochlorite for 15 minutes, they are then soaked in 50°C sterile water for 2 hours, and finally rinsed with clean water. The seeds are evenly spread on moist filter paper and left to germinate in the dark, and then sown in pots containing seedling substrate (purchased from Xiangzheng Agricultural Science) for 5 days, and then the seedlings with good and consistent growth are selected for transplanting. A total of 4 groups were set up in the potted test (each group was set up with 6 replicates, using a 48-hole seedling pot, and every 8 holes were 1 replicate), as follows:

[0066] Control group T1: administration of clean water;

[0067] Fertilizer group T2: using the fertilizer prepared in Comparative Example 1;

[0068] Organic fertilizer group T3: applying the organic fertilizer prepared in Comparative Example 2;

[0069] Single bacteria group T4: spore concentration was 5.0×10 8 CFU / mL of Trichoderma harzianum NJAU 4742 spore solution;

[0070] Trichoderma liquid organic fertilizer group T5: using the Trichoderma liquid organic fertilizer prepared in Example 1;

[0071] One crop was planted in each hole, and 80g of soil was placed in each hole. The total amount of fertilizer applied to each group was 2% of the total mass of the soil. Fertilizer was applied every 6 days and applied to the soil 5 times on average. Each fertilizer was diluted at a ratio of 1:60 before being applied to the soil at the root of the crop. During the potted plant experiment, the greenhouse planting conditions were maintained as follows: the photoperiod was 16h per day (120μmol photons m -2 ·s -1 ), temperature 26 / 23℃ (day / night). Water once every other day, 40mL per hole each time. No watering on the day of fertilization and adding pathogens. 20 days after transplanting, add cucumber wilt pathogen solution (5×10 6 CFU / mL of Fusarium oxysporum spore solution) was added. 17 days after inoculation of the Fusarium oxysporum spore solution, the severity of the disease in the cucumber plants was counted and divided into five levels: 0, 1, 2, 3, and 4.

[0072] Level 0 = no disease (no symptoms); Level 1 = 25% of leaves show wilt symptoms; Level 2 = 25%-50% of leaves show wilt symptoms; Level 3 = 50%-75% of leaves show wilt symptoms; Level 4 = 75%-100% of leaves show wilt symptoms.

[0073] The calculation formula of disease severity index (DSI) is as follows: disease severity index (DSI) = [∑ (number of diseased plants × disease severity) / (total number of plants × highest disease severity)] × 100

[0074] The results are as follows Figure 2 And as shown in Table 2.

[0075] Table 2 Effects of different fertilizer treatments on the disease grade and index of cucumber wilt

[0076]

[0077] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level

[0078] from Figure 2 As can be seen from the results in Table 2, the number of cucumber plants without disease in the Trichoderma liquid organic fertilizer group was the largest, and the number of cucumber plants with severe disease was the smallest; and the disease index of the Trichoderma liquid organic fertilizer group was significantly lower than that of the control group, chemical fertilizer group, organic fertilizer group and single bacteria group. The disease indexes of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 61.67%, 56.67%, 60.00%, 40.00% and 35.00%, respectively. Among them, the disease index of the Trichoderma liquid organic fertilizer group was 43.25% lower than that of the control group; 38.24% lower than that of the chemical fertilizer group; and 41.67% lower than that of the organic fertilizer group. The results show that the application of Trichoderma liquid organic fertilizer can significantly reduce the incidence of cucumber wilt.

[0079] (3) Effect of Trichoderma liquid organic fertilizer on resistance to low temperature stress

[0080] The effect of Trichoderma liquid organic fertilizer on the resistance of cucumber plants to low temperature stress was verified through greenhouse potted experiments.

[0081] Disinfection and germination of plant seeds: After the cucumber seeds were disinfected by soaking in 10% sodium hypochlorite for 15 minutes, they were then soaked in 50°C sterile water for 2 hours, and finally rinsed with clean water. The seeds were evenly spread on moist filter paper and left to germinate in the dark, and then sown in pots containing seedling medium (purchased from Xiangzheng Agricultural Science) for 5 days, and then the seedlings with good and consistent growth were selected for transplanting. A total of 4 groups were set up in the potted test (each group had 6 replicates, using a 48-hole seedling pot, and every 8 holes were 1 replicate), as follows:

[0082] Control group T1: administration of clean water;

[0083] Fertilizer group T2: using the fertilizer prepared in Comparative Example 1;

[0084] Organic fertilizer group T3: applying the organic fertilizer prepared in Comparative Example 2;

[0085] Single bacteria group T4: spore concentration was 5.0×10 8 CFU / mL of Trichoderma harzianum NJAU 4742 spore solution;

[0086] Trichoderma liquid organic fertilizer group T5: using the Trichoderma liquid organic fertilizer prepared in Example 1;

[0087] One crop was planted in each hole, and each hole was filled with 80g of soil. The total amount of fertilizer for each group was 2% of the total mass of the soil. Fertilizer was applied every two days and applied to the soil three times on average. Each time the fertilizer was diluted at a ratio of 1:60 and then applied to the soil at the root of the crop. Each treatment group was randomly placed during the experiment. After 7 days of transplanting, the cucumber seedlings were moved to a light incubator and treated for 2 days at a light intensity of 6000 / 1000Lx (8:00-20:00, light 12h) (temperature, day 1: 20℃ / 15℃ (day / night), day 2: 15℃ / 10℃ (day / night)), 12h each day and night, and then the temperature stress treatment was formally carried out, and the treatment temperature was 10℃ / 5℃ (day / night). A humidifier was used to keep the air humidity in the box at 70%. Samples were taken on the 5th day after the stress treatment and the seedling growth indicators were measured. The results are as follows Figure 3 And as shown in Table 3.

[0088] Table 3 Effects of different fertilizer treatments on the biological morphology of cucumber

[0089]

[0090] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level

[0091] from Figure 3As can be seen from the results in Table 3, the plant heights of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 13.21cm, 14.84cm, 14.97cm, 15.05cm and 15.55cm respectively, and the plant heights of the chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were significantly improved compared with the control group treatment, among which, the plant height of the Trichoderma liquid organic fertilizer group was increased by 17.71% compared with the control group. The stem diameters of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 2.67mcm, 2.82mm, 2.79mm, 2.98mm and 3.11mm respectively, and the root lengths were 15.20cm, 15.57cm, 17.90cm, 18.34cm and 20.10cm respectively. The stem diameter and root length of the Trichoderma liquid organic fertilizer group were significantly improved compared with the control group treatment, chemical fertilizer group treatment, organic fertilizer group and single bacteria group. Among them, the stem diameter of the Trichoderma liquid organic fertilizer group increased by 16.48% compared with the control group, 10.28% compared with the chemical fertilizer group, 12.29% compared with the organic fertilizer group, and 4.4% compared with the single bacteria group. The aboveground fresh weight of the control group, chemical fertilizer group, organic fertilizer group, single bacteria group and Trichoderma liquid organic fertilizer group were 6.05g, 6.37g, 6.77g, 6.94g and 7.47g respectively; the underground fresh weight was 0.50g, 0.60g, 0.80g, 0.91g and 1.05g respectively. The aboveground fresh weight and underground fresh weight of the Trichoderma liquid organic fertilizer group were significantly higher than those of the control group and the chemical fertilizer group. The aboveground fresh weight of the Trichoderma liquid organic fertilizer group was 23.47% higher than that of the control group, and the underground fresh weight was 110% higher than that of the control group. It shows that the application of Trichoderma liquid organic fertilizer can significantly increase the resistance of cucumber to low temperature stress.

[0092] In summary, the present invention provides a Trichoderma liquid organic fertilizer for promoting crop growth and enhancing the ability of crops to resist wilt disease and low temperature stress.

[0093] The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiments. It is understood that other improvements and changes directly derived or associated by those skilled in the art without departing from the spirit and concept of the present invention should be considered to be included in the protection scope of the present invention.

Claims

1. A liquid organic fertilizer of Trichoderma developed based on citric acid fermentation tail liquid, characterized in that: The Trichoderma liquid organic fertilizer comprises citric acid fermentation tail liquid, inorganic fertilizer and Trichoderma spores.

2. Trichoderma liquid organic fertilizer according to claim 1, is characterized in that, The Trichoderma spores are Trichoderma harzianum NJAU 4742 spores.

3. Trichoderma liquid organic fertilizer according to claim 1, is characterized in that, The organic matter content in the citric acid fermentation tail liquid is 21.6-23.7%.

4. Trichoderma liquid organic fertilizer according to claim 1, is characterized in that, The inorganic fertilizer is composed of urea and potassium sulfate; the mass ratio of urea to potassium sulfate is 116:

3.

5. Trichoderma liquid organic fertilizer according to claim 1, is characterized in that, The inorganic fertilizer is added in an amount of 13.2-25.5 g / L relative to the citric acid fermentation tail liquid.

6. Trichoderma liquid organic fertilizer according to claim 1, is characterized in that, The total nutrient content of the biological organic water-soluble fertilizer is 8-12%, the organic matter content is 20-22%, and the number of Trichoderma spores is 5×10 7 -5×10 8 CFU / mL, pH 5.5-6.

5.

7. Use of the Trichoderma liquid organic fertilizer according to any one of claims 1 to 6 in improving the ability of plants to resist low temperature stress.

8. Use of the Trichoderma liquid organic fertilizer according to any one of claims 1 to 6 in promoting plant growth.

9. Use of the Trichoderma liquid organic fertilizer according to any one of claims 1 to 6 in improving plant resistance to Fusarium wilt.

10. The use according to any one of claims 7 to 9, characterized in that: The plants include sunflower and cucumber.

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