Magnesium alloy surface bionic endothelial cell induction coating and preparation method thereof

Abstract

The invention discloses a magnesium alloy surface bionic endothelial cell induction coating and a preparation method thereof, and relates to the technical field of biomedical materials.The preparation method comprises the steps that raw materials are prepared, an oxidation film is formed on the magnesium alloy surface, a monomolecular film is formed on the surface of the oxidation film, and the bionic endothelial cell induction coating is formed on the magnesium alloy surface through a dip-coating method; according to the preparation method, a uniform and compact oxide layer is formed on the surface of the magnesium alloy metal sheet by adopting an alkali heat method so as to improve the corrosion resistance of the magnesium alloy metal sheet, and then a uniform and compact monomolecular film is formed on the oxide layer on the surface of the magnesium alloy metal sheet by adopting a thermal organic film method so as to provide additional protection, so that the corrosion resistance and the biocompatibility of the magnesium alloy metal sheet are further enhanced; and finally, preparing a composite bionic solution from bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water as raw materials, and taking the composite bionic solution as a bionic endothelial cell induction coating to endow the coating with good biodegradability and biocompatibility.

Description

Technical Field

[0001] The invention relates to the technical field of biomedical materials, and in particular to a bionic endothelial cell induction coating on the surface of a magnesium alloy and a preparation method thereof. Background Art

[0002] In the prior art, stainless steel and titanium-based alloys have been widely used in the field of clinical medicine as implant materials. However, these metal materials may release toxic ions due to corrosion by body fluids, thereby causing local allergies or inflammation. Magnesium and its alloys can effectively solve the above problems. Biomedical magnesium alloys refer to magnesium alloys used in the medical field, especially as implant materials. Because they have mechanical properties similar to those of human bones, good biocompatibility and biodegradability, they show great potential and application prospects in the biomedical field.

[0003] The bionic endothelial cell-inducing coating on the surface of magnesium alloy is a special surface coating that mimics the characteristics of human vascular endothelial cells and is designed to improve the compatibility of vascular stents or other implants with the inner wall of human blood vessels. The coating can induce vascular endothelial cells to grow and proliferate on its surface, thereby forming a layer of vascular endothelial layer with normal physiological functions, which helps prevent problems such as thrombosis and restenosis of blood vessels.

[0004] Existing bionic endothelial cell inducing coatings on magnesium alloy surfaces usually have the problems of single composition, complex preparation process, poor corrosion resistance or insufficient biocompatibility. These defects result in that the prepared bionic endothelial cell inducing coatings cannot effectively improve the corrosion resistance of magnesium alloys while ensuring good biocompatibility, thereby affecting the functionality of magnesium alloy implants and limiting their application in the field of medical materials. Therefore, the present invention proposes an improved bionic endothelial cell inducing coating on magnesium alloy surface and a preparation method thereof to solve the above problems existing in the prior art. Summary of the invention

[0005] In view of the above problems, the purpose of the present invention is to propose a bionic endothelial cell inducing coating on the surface of a magnesium alloy and a preparation method thereof, so as to solve the deficiencies of the existing bionic endothelial cell inducing coating on the surface of magnesium alloy in terms of corrosion resistance or biocompatibility, thereby ensuring that the magnesium alloy has good biocompatibility while improving its corrosion resistance.

[0006] In order to achieve the purpose of the present invention, the present invention adopts the following technical scheme: a bionic endothelial cell inducing coating on the surface of a magnesium alloy and a preparation method thereof, comprising the following raw materials in parts by weight: 0.5 to 1 part of a bioactive molecule, 0.5 to 1 part of an inorganic mineral, 1 to 2 parts of polylactic acid, 1 to 2 parts of polyhydroxyalkanoate, 7 to 8 parts of dichloromethane, 7 to 8 parts of acetone and 10 to 12 parts of water.

[0007] A further improvement is that the bioactive molecule is one of fibronectin and laminin, and the inorganic mineral is one of calcium phosphate and hydroxyapatite.

[0008] A method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface comprises the following steps:

[0009] Step 1: firstly prepare a magnesium alloy metal sheet according to actual preparation requirements and perform surface pretreatment on it, then respectively prepare bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water according to a mass ratio of 0.5-1:0.5-1:1-2:1-2:7-8:7-8:10-12, and prepare a composite bionic solution for use;

[0010] Step 2: placing the magnesium alloy metal sheet that has undergone surface pretreatment in step 1 in a potassium hydroxide solution for heating and soaking. After soaking, the magnesium alloy metal sheet is transferred to an electric heating furnace for heat treatment to form an oxide protective layer on its surface. The heat-treated magnesium alloy metal sheet is then taken out and washed with deionized water.

[0011] Step 3: soaking the magnesium alloy metal sheet cleaned in step 2 in an alkylthiol solvent, and after soaking, placing it in an electric heating furnace for heat treatment to form a monomolecular film on the surface oxide layer of the magnesium alloy metal sheet, and after heat treatment, taking out the magnesium alloy metal sheet and washing it with deionized water;

[0012] Step 4: Immerse the magnesium alloy metal sheet cleaned in step 3 into the composite bionic solution prepared in step 1, so that the composite bionic solution is evenly coated on the surface of the magnesium alloy metal sheet; then, place the dipped magnesium alloy metal sheet in a dryer for drying to evaporate the solvent and solidify the surface coating of the magnesium alloy metal sheet, thereby forming a bionic endothelial cell inducing coating.

[0013] A further improvement is that in step one, the specific steps of surface pretreatment of the magnesium alloy metal sheet are: first, use No. 240 sandpaper to polish the surface of the magnesium alloy metal sheet, and then use No. 400 sandpaper to polish the surface of the magnesium alloy metal sheet, and then ultrasonically clean the polished magnesium alloy metal sheet in distilled water and alcohol respectively, the cleaning time is 20 to 30 minutes, and then dry it after cleaning, and after drying, use ultraviolet light to irradiate the surface of the magnesium alloy metal sheet for 2 to 3 hours.

[0014] A further improvement is that in the step 1, the specific preparation method of the composite bionic solution is: first, polylactic acid, dichloromethane and half of water are mixed and stirred to obtain a first degradable polymer solution; then, polyhydroxyalkanoate, acetone and the other half of water are mixed and stirred to obtain a second degradable polymer solution; then, the first degradable polymer solution, the second degradable polymer solution, the bioactive molecules and the inorganic minerals are mixed and stirred, and ultrasonic treatment is performed; after the mixing and stirring is completed, the undissolved particles are filtered out to obtain the composite bionic solution.

[0015] A further improvement is that in step 2, the mass percentage concentration of the potassium hydroxide solution is 10-15%, the magnesium alloy metal sheet is placed in the potassium hydroxide solution, heated at a temperature of 50-65° C. and soaked for 40-60 minutes, and then the magnesium alloy metal sheet is placed in an electric heating furnace and heat treated at a temperature of 250-350° C. for 1-1.5 hours.

[0016] A further improvement is that in the step three, the alkyl mercaptan solvent is prepared by mixing alkyl mercaptan and ethanol in a mass ratio of 1 to 2:10 to 15, the magnesium alloy metal sheet is immersed in the alkyl mercaptan solvent for 30 to 50 minutes, and then the magnesium alloy metal sheet is placed in an electric furnace for heat treatment at a temperature of 125 to 175° C. for 1.5 to 2 hours.

[0017] A further improvement is that in step 4, the magnesium alloy metal sheet is repeatedly dipped in the composite bionic solution for 2 to 3 times, and the dipped magnesium alloy metal sheet is placed in a dryer and dried at a temperature of 80 to 100° C. for 30 to 40 minutes.

[0018] The beneficial effects of the present invention are as follows: the present invention first uses an alkali-thermal method to form a uniform and dense oxide layer on the surface of the magnesium alloy metal sheet to improve its corrosion resistance. Next, a thermal organic film method is used to form a uniform and dense monomolecular film on the oxide layer on the surface of the magnesium alloy metal sheet, thereby providing additional protection and further enhancing its corrosion resistance and biocompatibility. Finally, a composite bionic solution prepared using bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water as raw materials is used as a bionic endothelial cell inducing coating on the surface of the magnesium alloy, and the coating has good biodegradability and biocompatibility. The overall preparation process is simple and easy, which significantly improves the functionality of magnesium alloy implants and makes them more suitable for biomedical applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings required in the embodiments or the prior art description are briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. Ordinary technicians in this field can obtain other relevant drawings based on these drawings without creative work.

[0020] Figure 1 The present invention is a schematic flow chart of a method for preparing a bionic endothelial cell-inducing coating on a magnesium alloy surface. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should be regarded as the scope of protection of the present invention.

[0022] Embodiment 1

[0023] The present embodiment provides a bionic endothelial cell inducing coating on the surface of a magnesium alloy and a preparation method thereof, comprising the following raw materials in parts by weight: 0.5 parts of a bioactive molecule, 1 part of an inorganic mineral, 1 part of polylactic acid, 2 parts of polyhydroxyalkanoate, 7 parts of dichloromethane, 8 parts of acetone and 10 parts of water, wherein the bioactive molecule is fibronectin and the inorganic mineral is calcium phosphate. In the present embodiment, fibronectin is used as the bioactive molecule and calcium phosphate is used as the inorganic mineral, and polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water are used as auxiliary materials to prepare a composite bionic solution as a bionic endothelial cell inducing coating on the surface of a magnesium alloy, so that the coating has good biodegradability and biocompatibility.

[0024] See also Figure 1 This embodiment also provides a method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface, comprising the following steps:

[0025] Step 1: Raw material preparation

[0026] First, a magnesium alloy metal sheet is prepared according to actual preparation requirements, and is cut according to actual preparation requirements. Then, the surface of the magnesium alloy metal sheet is polished with No. 240 sandpaper, and then with No. 400 sandpaper to improve the surface adhesion of the magnesium alloy metal sheet. After polishing, the magnesium alloy metal sheet is first ultrasonically cleaned in distilled water for 20 minutes, and then ultrasonically cleaned in alcohol for 20 minutes. After cleaning, the magnesium alloy metal sheet is dried and irradiated under ultraviolet light for 2 hours to achieve disinfection and sterilization to ensure the cleanliness of the magnesium alloy metal sheet.

[0027] Weigh the bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water in a mass ratio of 0.5:1:1:1:7:8:10, mix the polylactic acid, dichloromethane and half of the water prepared above, and stir evenly to obtain a first degradable polymer solution for standby use, then mix the polyhydroxyalkanoate, acetone and the remaining half of the water prepared above, and stir evenly to obtain a second degradable polymer solution, mix the first degradable polymer solution, the second degradable polymer solution, the bioactive molecules and the inorganic minerals, and perform ultrasonic treatment during the mixing and stirring process to promote the dissolution of the substances, after mixing and stirring, filter out the undissolved particles therein to ensure the purity of the solution, and obtain a composite bionic solution for subsequent dip coating treatment on the surface of the magnesium alloy metal sheet;

[0028] Step 2: Forming an oxide film on the surface of the magnesium alloy

[0029] The magnesium alloy metal sheet after surface pretreatment is immersed in a potassium hydroxide solution with a mass percentage concentration of 10%, and heated and immersed at 50° C. for 40 minutes. After the immersion is completed, the magnesium alloy metal sheet is taken out and placed in an electric heating furnace, and heat-treated at 250° C. for 1 hour to form an oxidation protective layer on the surface of the magnesium alloy metal sheet. Subsequently, the magnesium alloy metal sheet after heat treatment is taken out and washed with deionized water. In this embodiment, an alkaline heat method is used to form a uniform and dense oxide layer on the surface of the magnesium alloy metal sheet, thereby improving its corrosion resistance and enhancing its applicability in biomedical applications;

[0030] Step 3: Forming a monomolecular film on the surface of the oxide film

[0031] First, the magnesium alloy metal sheet with an oxide layer formed on the surface is immersed in an alkylthiol solvent for 30 minutes, wherein the alkylthiol solvent is prepared by mixing alkylthiol and ethanol in a mass ratio of 1:10. After the immersion is completed, the magnesium alloy metal sheet is taken out and placed in an electric heating furnace, and heat-treated at 125° C. for 1.5 hours to form a monomolecular film on the oxide layer on the surface of the magnesium alloy metal sheet. Subsequently, the heat-treated magnesium alloy metal sheet is taken out and washed with deionized water. In this embodiment, a uniform and dense monomolecular film is formed on the oxide layer on the surface of the magnesium alloy metal sheet by a thermal organic film method, thereby providing additional protection and further enhancing its corrosion resistance and biocompatibility.

[0032] Step 4: Forming a bionic endothelial cell-inducing coating on the surface of the magnesium alloy by dip coating

[0033] A magnesium alloy metal sheet with an oxide film and a monomolecular film formed on the surface is immersed in a composite bionic solution, and the composite bionic solution is used to repeatedly dip-coat the magnesium alloy metal sheet twice to ensure that the composite bionic solution is evenly dip-coated on the surface of the magnesium alloy metal sheet. Subsequently, the dip-coated magnesium alloy metal sheet is placed in a dryer and heated and dried at 80°C for 30 minutes to volatilize the solvent on the surface of the magnesium alloy metal sheet and solidify the dip-coated coating on the surface to form a bionic endothelial cell inducing coating, which can simulate the environment of human endothelial cells, promote the adhesion and growth of endothelial cells, thereby improving the biocompatibility of magnesium alloy implants, and at the same time can control the degradation rate of the magnesium alloy to ensure that it matches the healing process of the surrounding tissue.

[0034] Embodiment 2

[0035] The present embodiment provides a bionic endothelial cell inducing coating on the surface of a magnesium alloy and a preparation method thereof, comprising the following raw materials in parts by weight: 1 part of a bioactive molecule, 0.5 parts of an inorganic mineral, 2 parts of polylactic acid, 1 part of a polyhydroxyalkanoate, 8 parts of dichloromethane, 7 parts of acetone and 12 parts of water, wherein the bioactive molecule is laminin and the inorganic mineral is hydroxyapatite. In the present embodiment, laminin is used as the bioactive molecule and hydroxyapatite is used as the inorganic mineral, and polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water are used as auxiliary materials to prepare a composite bionic solution as a bionic endothelial cell inducing coating on the surface of a magnesium alloy, so that the coating has good biodegradability and biocompatibility.

[0036] See also Figure 1 This embodiment also provides a method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface, comprising the following steps:

[0037] Step 1: Raw material preparation

[0038] According to the actual preparation needs, the magnesium alloy metal sheet is prepared, and the size of the magnesium alloy metal sheet is cut according to the actual preparation needs. Then, the surface of the magnesium alloy metal sheet is polished with No. 240 sandpaper, and then the surface of the magnesium alloy metal sheet is polished with No. 400 sandpaper to improve the surface adhesion of the magnesium alloy metal sheet. After polishing, it is first ultrasonically cleaned in distilled water for 30 minutes, and then ultrasonically cleaned in alcohol for 30 minutes. After cleaning, the magnesium alloy metal sheet is dried and irradiated under ultraviolet light for 3 hours to achieve the purpose of disinfection and sterilization to ensure the cleanliness of the magnesium alloy metal sheet;

[0039] Prepare bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water in a mass ratio of 1:0.5:2:1:8:7:12, then mix and stir the prepared polylactic acid, dichloromethane and half of the water to obtain a first degradable polymer solution for standby use; then, mix and stir the prepared polyhydroxyalkanoate, acetone and the remaining half of the water to obtain a second degradable polymer solution; then, mix and stir the first degradable polymer solution, the second degradable polymer solution, the bioactive molecules and the inorganic minerals, and perform ultrasonic treatment during the mixing and stirring process to promote the dissolution of the substances therein, and filter out the undissolved particles after the mixing and stirring to ensure the purity of the solution, so as to obtain a composite bionic solution for subsequent dip coating treatment on the surface of the magnesium alloy metal sheet;

[0040] Step 2: Forming an oxide film on the surface of the magnesium alloy

[0041] The magnesium alloy metal sheet that has undergone surface pretreatment is placed in a potassium hydroxide solution with a mass percentage concentration of 15%, and heated and soaked at 65° C. for 60 minutes. After soaking, the magnesium alloy metal sheet is taken out and placed in an electric heating furnace, and heat-treated at 350° C. for 1.5 hours to form an oxidation protective layer on the surface of the magnesium alloy metal sheet. Subsequently, the magnesium alloy metal sheet after heat treatment is taken out and washed with deionized water. In this embodiment, an alkaline heat method is used to form a uniform and dense oxide layer on the surface of the magnesium alloy metal sheet, thereby improving its corrosion resistance to a certain extent and enhancing its applicability in biomedical applications.

[0042] Step 3: Forming a monomolecular film on the surface of the oxide film

[0043] The magnesium alloy metal sheet with an oxide layer formed on the surface is immersed in an alkylthiol solvent for 50 minutes, wherein the alkylthiol solvent is prepared by mixing alkylthiol and ethanol in a mass ratio of 2:15. After the immersion is completed, the magnesium alloy metal sheet is taken out and placed in an electric heating furnace, and heat-treated at 175° C. for 2 hours to form a monomolecular film on the surface oxide layer of the magnesium alloy metal sheet. After the heat treatment, the magnesium alloy metal sheet is taken out and washed with deionized water. In this embodiment, a uniform and dense monomolecular film is formed on the oxide layer on the surface of the magnesium alloy metal sheet by a thermal organic film method to provide additional protection and improve its corrosion resistance and biocompatibility.

[0044] Step 4: Form a bionic endothelial cell inducing coating on the surface of the magnesium alloy by dip coating. Immerse the magnesium alloy metal sheet with an oxide film and a monomolecular film formed on the surface into the composite bionic solution, and repeat the dip coating for 3 times to ensure that the composite bionic solution is evenly dipped on the surface of the magnesium alloy metal sheet. Subsequently, place the dipped magnesium alloy metal sheet in a drying oven and dry it at 100°C for 40 minutes to volatilize the surface solvent and solidify the dip-coated coating on the surface to form a bionic endothelial cell inducing coating. The coating simulates the endothelial cell environment of the human body, promotes the adhesion and growth of endothelial cells, thereby improving the biocompatibility of magnesium alloy implants and controlling the degradation rate of the magnesium alloy to ensure that it matches the healing process of the surrounding tissue.

[0045] The bionic endothelial cell inducing coatings on the surface of magnesium alloys prepared in Examples 1 and 2 of the present invention were taken as Sample Example 1 and Sample Example 2, respectively. The preparation methods of Examples 1 and 2 of the present invention were adopted, and Steps 2 and 3 therein were omitted. Under the condition that other conditions remained unchanged, bionic endothelial cell inducing coatings on the surface of magnesium alloys were prepared as Comparative Examples 1 and 2, respectively. Electrochemical impedance spectroscopy (EIS) was used to measure the impedance changes of Sample Example 1, Sample Example 2, Comparative Example 1 and Comparative Example 2 in simulated body fluids, and the corrosion resistance of each coating was evaluated. The results showed that the corrosion rates of Sample Example 1 and Sample Example 2 were half of the corrosion rates of Comparative Example 1 and Comparative Example 2, respectively. The corrosion resistance test results are shown in Table 1 below. The platelet activation and coagulation test methods were used to evaluate the effects of the coatings of Sample Example 1, Sample Example 2, Comparative Example 1 and Comparative Example 2 on platelet activation and coagulation processes. The evaluation results showed that Sample Example 1 and Sample Example 2 had better biocompatibility than Comparative Example 1 and Comparative Example 2.

[0046] Table 1 Corrosion resistance test comparison table

[0047]

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims

1. A bionic endothelial cell inducing coating on the surface of a magnesium alloy, characterized in that: The invention comprises the following raw materials in parts by weight: 0.5 to 1 part of biologically active molecules, 0.5 to 1 part of inorganic minerals, 1 to 2 parts of polylactic acid, 1 to 2 parts of polyhydroxyalkanoate, 7 to 8 parts of dichloromethane, 7 to 8 parts of acetone and 10 to 12 parts of water.

2. The bionic endothelial cell inducing coating on the surface of a magnesium alloy according to claim 1, characterized in that: The bioactive molecule is one of fibronectin and laminin, and the inorganic mineral is one of calcium phosphate and hydroxyapatite.

3. A method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface as claimed in claim 1, characterized in that: The following steps are involved: Step 1: firstly prepare a magnesium alloy metal sheet according to actual preparation requirements and perform surface pretreatment on it, then respectively prepare bioactive molecules, inorganic minerals, polylactic acid, polyhydroxyalkanoate, dichloromethane, acetone and water according to a mass ratio of 0.5-1:0.5-1:1-2:1-2:7-8:7-8:10-12, and prepare a composite bionic solution for use; Step 2: placing the magnesium alloy metal sheet that has undergone surface pretreatment in step 1 in a potassium hydroxide solution for heating and soaking. After soaking, the magnesium alloy metal sheet is transferred to an electric heating furnace for heat treatment to form an oxide protective layer on its surface. The heat-treated magnesium alloy metal sheet is then taken out and washed with deionized water. Step 3: soaking the magnesium alloy metal sheet cleaned in step 2 in an alkylthiol solvent, and after soaking, placing it in an electric heating furnace for heat treatment to form a monomolecular film on the surface oxide layer of the magnesium alloy metal sheet, and after heat treatment, taking out the magnesium alloy metal sheet and washing it with deionized water; Step 4: Immerse the magnesium alloy metal sheet cleaned in step 3 into the composite bionic solution prepared in step 1, so that the composite bionic solution is evenly coated on the surface of the magnesium alloy metal sheet; then, place the dipped magnesium alloy metal sheet in a dryer for drying to evaporate the solvent and solidify the surface coating of the magnesium alloy metal sheet, thereby forming a bionic endothelial cell inducing coating.

4. The method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface according to claim 3, characterized in that: In the step 1, the specific steps of surface pretreatment of the magnesium alloy metal sheet are: first, use No. 240 sandpaper to polish the surface of the magnesium alloy metal sheet, and then use No. 400 sandpaper to polish the surface of the magnesium alloy metal sheet, and then ultrasonically clean the polished magnesium alloy metal sheet in distilled water and alcohol respectively, the cleaning time is 20 to 30 minutes, and then dry it after cleaning, and after drying, use ultraviolet light to irradiate the surface of the magnesium alloy metal sheet for 2 to 3 hours.

5. The method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface according to claim 3, characterized in that: In the step 1, the specific preparation method of the composite biomimetic solution is: first, polylactic acid, dichloromethane and half of water are mixed and stirred to obtain a first degradable polymer solution; then, polyhydroxyalkanoate, acetone and the other half of water are mixed and stirred to obtain a second degradable polymer solution; then, the first degradable polymer solution, the second degradable polymer solution, the bioactive molecule and the inorganic mineral are mixed and stirred, and ultrasonic treatment is performed; After the mixing and stirring is completed, undissolved particles are filtered out to obtain a composite bionic solution.

6. The method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface according to claim 3, characterized in that: In the step 2, the mass percentage concentration of the potassium hydroxide solution is 10-15%, the magnesium alloy metal sheet is placed in the potassium hydroxide solution, heated at a temperature of 50-65° C. and soaked for 40-60 minutes, and then the magnesium alloy metal sheet is placed in an electric heating furnace and heat treated at a temperature of 250-350° C. for 1-1.5 hours.

7. The method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface according to claim 3, characterized in that: In the step three, the alkyl mercaptan solvent is prepared by mixing alkyl mercaptan and ethanol in a mass ratio of 1-2:10-15, the magnesium alloy metal sheet is immersed in the alkyl mercaptan solvent for 30-50 minutes, and then the magnesium alloy metal sheet is placed in an electric heating furnace for heat treatment at a temperature of 125-175° C. for 1.5-2 hours.

8. The method for preparing a bionic endothelial cell inducing coating on a magnesium alloy surface according to claim 3, characterized in that: In the step 4, the magnesium alloy metal sheet is repeatedly dipped in the composite bionic solution for 2 to 3 times, and the dipped magnesium alloy metal sheet is placed in a dryer and dried at a temperature of 80 to 100° C. for 30 to 40 minutes.

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