Whitening and anti-wrinkle composition as well as preparation method and application thereof

Abstract

The invention provides a whitening and anti-wrinkle composition as well as a preparation method and application thereof. The whitening and anti-wrinkle composition is prepared from the following components in percentage by total mass of 100%: 80-98% of spirulina extract; the content of the hydroxypropyl tetrahydropyrantriol is 0.05 to 10 percent; and 0.01 to 5 percent of small molecule peptide. The whitening and anti-wrinkle composition disclosed by the invention has excellent whitening and anti-wrinkle effects. The whitening and anti-wrinkle composition disclosed by the invention is safe, mild and quick in effect when being applied to skin care products.

Description

Technical Field

[0001] The invention relates to a whitening and anti-wrinkle composition and a preparation method and application thereof, belonging to the field of cosmetics. Background Art

[0002] So far, products with strong whitening and anti-wrinkle effects are mainly divided into two categories: natural products and chemically synthesized products. However, many chemically synthesized ingredients with whitening and anti-aging effects, such as arbutin, kojic acid, ascorbic acid and retinoic acid, have been reported to have side effects to a certain extent. For example, although ascorbic acid and kojic acid have good whitening effects, they have poor stability, low skin penetration ability, and can cause cytotoxicity, dermatitis and erythema after long-term use. Retinoic acid is highly irritating and requires tolerance. When used by people with sensitive skin, the skin sensitivity is more serious. Therefore, mild and effective whitening and anti-wrinkle ingredients that can reduce skin pigmentation and wrinkles are becoming more and more popular. Researching mild whitening and anti-wrinkle ingredients and applying them to cosmetics or skin care products can provide a theoretical basis for the utilization of functional ingredients in cosmetics or skin care products.

[0003] The polyphenol content and biological activity produced by algae depend not only on the growth environment and climatic conditions, but also on the extraction method and operation process. Traditional extraction methods, such as cold soaking, warm extraction, cold soaking and grinding, Soxhlet extraction, water-alcohol extraction, etc., have relatively low polyphenol content, which makes the efficacy of algae extracts unable to be effectively exerted. Summary of the invention

[0004] Problem that the invention aims to solve

[0005] In view of the problems existing in the prior art, the present invention first provides a whitening and anti-wrinkle composition. The whitening and anti-wrinkle composition of the present invention has excellent whitening and anti-wrinkle effects.

[0006] The present invention also provides a method for preparing the whitening and anti-wrinkle composition. The preparation method is simple and easy to implement, the raw materials are easy to obtain, and the method is suitable for mass production.

[0007] The present invention also provides use of the whitening and anti-wrinkle composition in cosmetics or skin care products.

[0008] Solutions for solving problems

[0009] The present invention provides a whitening and anti-wrinkle composition, wherein, based on the total mass of the whitening and anti-wrinkle composition being 100%, the composition comprises the following components:

[0010] Spirulina extract: 80-98%;

[0011] Hydroxypropyl tetrahydropyrantriol: 0.05-10%;

[0012] Small molecule peptides: 0.01~5%.

[0013] According to the whitening and anti-wrinkle composition of the present invention, the spirulina extract is derived from Spirulina species, and the preservation number of the Spirulina species is CCTCC NO.: M 2017772.

[0014] According to the whitening and anti-wrinkle composition of the present invention, the spirulina species is extracted using a low eutectic solvent to obtain the spirulina extract;

[0015] Preferably, the low eutectic solvent comprises a hydrogen bond acceptor and a hydrogen bond donor; wherein the hydrogen bond acceptor comprises a quaternary ammonium salt compound, and the hydrogen bond donor comprises a carboxylic acid compound and / or a polyol;

[0016] More preferably, the extraction temperature is 55-70° C., and the extraction time is 0.5-3 hours.

[0017] According to the whitening and anti-wrinkle composition of the present invention, the hydrogen bond acceptor comprises choline chloride, and the hydrogen bond donor comprises one or a combination of two or more of oxalic acid, malic acid, ethylene glycol and citric acid.

[0018] According to the whitening and anti-wrinkle composition of the present invention, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:0.5-3.

[0019] According to the whitening and anti-wrinkle composition of the present invention, the hydroxypropyl tetrahydropyrantriol includes S-configuration hydroxypropyl tetrahydropyrantriol and / or R-configuration hydroxypropyl tetrahydropyrantriol.

[0020] According to the whitening and anti-wrinkle composition of the present invention, the small molecule peptide contains 2 to 10 amino acids; preferably, the small molecule peptide includes one or a combination of two or more of palmitoyl tripeptide-5, carnosine, palmitoyl pentapeptide-3, and acetyl hexapeptide-3.

[0021] The present invention also provides a method for preparing the whitening and anti-wrinkle composition according to the present invention, which comprises the step of mixing the components of the whitening and anti-wrinkle composition.

[0022] The present invention also provides a use of the whitening and anti-wrinkle composition according to the present invention for preparing cosmetics or skin care products; preferably, the skin care products include skin care lotions, skin care essences, face creams, sunscreens, BB creams, isolation creams or facial masks; the cosmetics include liquid foundation, concealer, blush, eye shadow or lipstick.

[0023] The present invention also provides an emulsified system, which comprises the whitening and anti-wrinkle composition according to the present invention; preferably, based on the total mass of the emulsified system, the amount of the whitening and anti-wrinkle composition added is 0.1-5%.

[0024] Effects of the Invention

[0025] The whitening and anti-wrinkle composition of the present invention has excellent whitening and anti-wrinkle effects. The whitening and anti-wrinkle composition of the present invention is safe, mild and effective in skin care product applications.

[0026] The preparation method of the whitening and anti-wrinkle composition of the present invention is simple and easy, the raw materials are easy to obtain, and it is suitable for mass production.

[0027] The present invention also provides use of the whitening and anti-wrinkle composition in cosmetics or skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The tyrosinase inhibition rates of the whitening and anti-wrinkle compositions of Application Examples 1-6 and Comparative Examples 1-6 of the present invention are shown.

[0029] Figure 2 The DPPH scavenging rates of the whitening and anti-wrinkle compositions of Application Examples 1-6 and Comparative Examples 1-6 of the present invention are shown.

[0030] Figure 3 The tyrosinase inhibition rates of the whitening and anti-wrinkle composition of Application Example 1 of the present invention and the tranexamic acid of Comparative Example 7 at different mass concentrations (0.1%, 0.3%, 0.5%, 0.7%, 0.9%) are shown.

[0031] Figure 4 The DPPH scavenging rates of the whitening and anti-wrinkle composition of Application Example 1 of the present invention and the tranexamic acid of Comparative Example 7 at different mass concentrations (0.1%, 0.3%, 0.5%, 0.7%, 0.9%) are shown. DETAILED DESCRIPTION

[0032] Various exemplary embodiments, features and aspects of the present invention will be described in detail below. The word "exemplary" used here means "used as an example, embodiment or illustrative". Any embodiment described here as "exemplary" is not necessarily interpreted as being superior or better than other embodiments.

[0033] In addition, in order to better illustrate the present invention, numerous specific details are provided in the following specific embodiments. It should be understood by those skilled in the art that the present invention can be implemented without certain specific details. In other examples, methods, means, equipment and steps well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present invention.

[0034] Unless otherwise stated, the units used in this specification are all international standard units, and the numerical values ​​and numerical ranges appearing in the present invention should be understood to include the inevitable systematic errors in industrial production.

[0035] In this specification, the word "may" means both performing a certain process and not performing a certain process.

[0036] In this specification, the references to "some specific / preferred embodiments", "other specific / preferred embodiments", "embodiments", etc., mean that the specific elements (e.g., features, structures, properties and / or characteristics) described in connection with the embodiments are included in at least one embodiment described herein, and may or may not exist in other embodiments. In addition, it should be understood that the elements may be combined in various embodiments in any suitable manner.

[0037] In this specification, the numerical range expressed using "a numerical value A to a numerical value B" means a range including the endpoints numerical values ​​A and B.

[0038] In addition, in this specification, the "water" includes any feasible water that can be used in the cosmetic field, such as deionized water, distilled water, ion exchange water, double distilled water, high-purity water, and purified water.

[0039] <First aspect>

[0040] The first aspect of the present invention provides a whitening and anti-wrinkle composition, which, based on the total mass of the whitening and anti-wrinkle composition as 100%, comprises the following components:

[0041] Spirulina extract: 80-98%;

[0042] Hydroxypropyl tetrahydropyrantriol: 0.05-10%;

[0043] Small molecule peptides: 0.01~5%.

[0044] The whitening and anti-wrinkle composition of the present invention has excellent whitening and anti-wrinkle effects. The whitening and anti-wrinkle composition of the present invention is safe, mild and effective in skin care product applications.

[0045] Spirulina Extract

[0046] The spirulina extract of the present invention is derived from spirulina species, and the spirulina species of the present invention is preferably spirulina bred in space, and its polyphenol, amino acid, polysaccharide and other contents are greatly increased, while more potential active effects are excavated, such as whitening and anti-wrinkle effects. Specifically, the deposit number of the spirulina species is CCTCC NO.: M2017772.

[0047] In some specific embodiments, the Spirulina species is extracted using a deep eutectic solvent to obtain a Spirulina extract. The inventors of the present invention have found that the use of a deep eutectic solvent to extract polyphenol components from seaweed can not only increase the content of monomeric polyphenols, but also extract active ingredients such as flavonoids and carotene, and the antioxidant effects of the Spirulina extract are more significant.

[0048] Deep eutectic solvents have the characteristics of low toxicity, adjustability, environmental friendliness, and low cost. Compared with traditional ionic liquids, deep eutectic solvent components can more effectively extract active substances and better maintain their stability.

[0049] In some specific embodiments, the low eutectic solvent includes a hydrogen bond acceptor and a hydrogen bond donor; wherein the hydrogen bond acceptor includes a quaternary ammonium salt compound, and the hydrogen bond donor includes a carboxylic acid compound and / or a polyol. Specifically, the hydrogen bond acceptor includes choline chloride, and the hydrogen bond donor includes one or a combination of two or more of oxalic acid, malic acid, ethylene glycol, and citric acid.

[0050] The inventors of the present invention have found that when choline chloride is used as a hydrogen bond acceptor and malic acid is used as a hydrogen bond donor, the polyphenol content of the prepared Spirulina extract is higher than that of other deep eutectic solvent combinations and the traditional ethanol reflux method. Therefore, the present invention preferably uses choline chloride as a hydrogen bond acceptor and malic acid as a hydrogen bond donor to increase the content of the active ingredient in the Spirulina extract.

[0051] In some specific embodiments, the extraction temperature is 55-70°C, for example: 58°C, 60°C, 62°C, 65°C, 68°C, etc.; the extraction time is 0.5-3 hours, for example: 0.8 hours, 1 hour, 1.2 hours, 1.5 hours, 1.8 hours, 2 hours, 2.2 hours, 2.5 hours, 2.8 hours, etc. When the extraction temperature is 60-65°C and the extraction time is 1-2 hours, it is easier to obtain the desired Spirulina extract.

[0052] In some specific embodiments, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:0.5-3, for example: 1:0.8, 1:1, 1:1.2, 1:1.5, 1:1.8, 1:2, 1:2.2, 1:2.5, 1:2.8, etc. When the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:0.5-3, the content of the effective ingredient of the obtained Spirulina extract is higher.

[0053] In addition, when used, the hydrogen bond acceptor and the hydrogen bond donor generally need to be dissolved in water. Specifically, the molar ratio of the hydrogen bond acceptor, the hydrogen bond donor and water is 1:0.5-3:1-5.

[0054] In some specific embodiments, the preparation method of the spirulina extract comprises the following steps: weighing 1.0-5.0 g of dried spirulina powder, measuring 10-50 mL of a low eutectic solvent, and mixing them evenly. The mixture is placed in a 50-100 mL round-bottom flask, the temperature is controlled at 55-70° C., and the mixture is heated and stirred for 0.5-3 h with a magnetic heating stirrer for extraction. After the extraction is completed, the spirulina extract is obtained by suction filtration.

[0055] In the present invention, based on the total mass of the whitening and anti-wrinkle composition being 100%, the content of the spirulina extract is 80-98%, for example, 82%, 85%, 88%, 90%, 92%, 95%, etc. When the content of the spirulina extract is less than 80%, it can play a certain whitening and anti-wrinkle role, but its role cannot be played to the best; when the content of the spirulina extract is more than 98%, the synergistic effect between the spirulina extract and hydroxypropyl tetrahydropyrantriol and the small molecule peptide is not obvious.

[0056] Hydroxypropyl Tetrahydropyrantriol

[0057] Hydroxypropyl tetrahydropyrantriol can effectively promote the synthesis of epidermal cell CD44 protein (a cross-glycoprotein on the cell surface that mediates the adhesion between cells and the extracellular matrix), perlecan, and svndecan, maintain the moisture content of the extracellular matrix, and reduce skin moisture loss; at the same time, it promotes the production of type IV collagen and laminin in the dermal-epidermal junction (DEJ), increases the firmness of the epidermis and the dermal-epidermal junction, and makes the skin more plump, full, and elastic.

[0058] In some specific embodiments, the hydroxypropyl tetrahydropyrantriol includes S-configuration hydroxypropyl tetrahydropyrantriol and / or R-configuration hydroxypropyl tetrahydropyrantriol.

[0059] The inventors of the present invention have found that S-configuration hydroxypropyl tetrahydropyrantriol can penetrate the stratum corneum and dermis of the skin, enter the interior of the cell, and participate in the synthesis of glycosaminoglycan (GAG) as the core primer, thereby promoting the secretion of soluble glycosaminoglycan. Therefore, the present invention preferably uses S-configuration hydroxypropyl tetrahydropyrantriol.

[0060] In the present invention, based on the total mass of the whitening and anti-wrinkle composition as 100%, the content of the hydroxypropyl tetrahydropyrantriol is 0.05-10%, for example: 0.1%, 0.5%, 1%, 3%, 5%, 7%, etc. When the content of the hydroxypropyl tetrahydropyrantriol is less than 0.05%, its efficacy cannot be effectively exerted; when the content of the hydroxypropyl tetrahydropyrantriol is greater than 10%, it cannot achieve a better effect and the cost is too high.

[0061] Small molecule peptides

[0062] Peptides are compounds formed by connecting α-amino acids in the form of peptide bonds. Peptides are a biochemical substance between amino acids and proteins. They have a smaller molecular weight than proteins and amino acids, and are a protein fragment. Peptides are highly active and can exert anti-aging effects at very low dosages.

[0063] In the present invention, the small molecule peptide refers to a polypeptide containing 2 to 10 amino acids; preferably, the small molecule peptide includes one or a combination of two or more of palmitoyl tripeptide-5, carnosine, palmitoyl pentapeptide-3, and acetyl hexapeptide-3.

[0064] Specifically, the present invention preferably uses palmitoyl tripeptide-5, which is a synthetic signal peptide that can mimic the sequence of thrombospondin 1, causing the sequence KRFK to bind to inactive transforming growth factor β (TGFβ), leading to the release of active TGFβ and promoting collagen synthesis. It can also inhibit the production of melanin in melanocytes by inhibiting tyrosinase activity and downregulating the mRNA levels of MITF and tyrosinase.

[0065] In the present invention, based on the total mass of the whitening and anti-wrinkle composition as 100%, the content of the small molecule peptide is 0.01-5%, for example: 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, etc. When the content of the small molecule peptide is less than 0.01%, it cannot produce a synergistic effect with the spirulina extract and hydroxypropyl tetrahydropyrantriol, and cannot effectively play the anti-aging effect; and it does not have a whitening effect; when the content of the small molecule peptide is greater than 5%, it will lead to excessively high costs.

[0066] <Second Aspect>

[0067] The second aspect of the present invention provides a method for preparing the whitening and anti-wrinkle composition according to the first aspect of the present invention, which comprises the step of mixing the components of the whitening and anti-wrinkle composition.

[0068] Specifically, the preparation method of the whitening and anti-wrinkle composition comprises adding spirulina extract, hydroxypropyl tetrahydropyrantriol and small molecule peptide into a container in sequence, stirring while adding until the mixture is evenly mixed.

[0069] <Third Aspect>

[0070] The third aspect of the present invention provides a use of the whitening and anti-wrinkle composition according to the first aspect of the present invention for preparing cosmetics or skin care products; preferably, the skin care products include skin care lotions, skin care essences, facial creams, sunscreens, BB creams, isolation creams or facial masks; the cosmetics include liquid foundation, concealer, blush, eye shadow or lipstick.

[0071] The present invention also provides an emulsified system, comprising the whitening and anti-wrinkle composition according to the present invention; preferably, based on the total mass of the emulsified system, the whitening and anti-wrinkle composition is added in an amount of 0.1-5%, for example: 0.2%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, etc. Preferably, the emulsified system can be an emulsion or a cream.

[0072] Example

[0073] The embodiments of the present invention will be described in detail below in conjunction with the examples, but those skilled in the art will appreciate that the following examples are only used to illustrate the present invention and should not be considered to limit the scope of the present invention. If no specific conditions are specified in the examples, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be obtained commercially.

[0074] Preparation of Spirulina Extract

[0075] Example

[0076] Weigh 1.0 g of dried Spirulina powder, measure 10 mL of a low eutectic solvent (the molar ratio of choline chloride, different hydrogen bond donors shown in Table 1, and water is 1:1:2), and mix well. Place the mixture in a 50 mL round-bottom flask, control the temperature at 60-65°C, and heat and stir with a magnetic heating stirrer for 1 hour for extraction. After the extraction is completed, filter and obtain the Spirulina extract.

[0077] Comparative Example

[0078] Use the commonly used ethanol reflux method to extract Spirulina. Weigh 1.0g of dry Spirulina powder and put it into a round-bottom flask. Add 10mL of 70% ethanol and heat to reflux. Take 3mL of the extract after 2 hours from the beginning of slight boiling and store it in a dark place.

[0079] Polyphenol content test

[0080] The Spirulina extract was diluted 10 times, 1 mL of each was added to a stoppered test tube (1 mL of distilled water was taken as a blank control), 1 mL of distilled water was added to each tube, 0.5 mL of a 1-fold diluted Folin-phenol solution was added (0.5 mL of distilled water was taken as a negative control), and 3 mL of a 13.4% Na2CO3 solution was added at the same time, and the volume was made up to 10 mL with distilled water. The reaction was carried out at room temperature in the dark for 2 h, and the absorbance at a wavelength of 760 nm was measured. The results of the polyphenol content are shown in Table 1.

[0081] Table 1

[0082]

[0083] As can be seen from Table 1, the polyphenol content in Spirulina obtained by using a variety of different deep eutectic solvents is higher than that of other deep eutectic solvent combinations and the traditional ethanol reflux method, so the use of deep eutectic solvent extraction can increase the content of effective ingredients in Spirulina extract. When choline chloride is used as a hydrogen bond acceptor and malic acid is used as a hydrogen bond donor as a deep eutectic solvent for extraction, the polyphenol obtained is higher.

[0084] In the following examples of the preparation of the whitening and anti-wrinkle composition, a Spirulina extract prepared using choline chloride as a hydrogen bond acceptor and malic acid as a hydrogen bond donor was used, and the remaining conditions were the same as those of the Spirulina extract prepared in the above examples.

[0085] Preparation of whitening and anti-wrinkle composition

[0086] Example 1

[0087] 95% of spirulina extract, 4.9% of S-configuration hydroxypropyl tetrahydropyrantriol, and 0.1% of palmitoyl tripeptide-5 were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0088] Example 2

[0089] 92% of spirulina extract, 7% of S-configuration hydroxypropyl tetrahydropyrantriol, and 1% of palmitoyl tripeptide-5 were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0090] Example 3

[0091] 95% of spirulina extract, 4% of S-configuration hydroxypropyl tetrahydropyrantriol, and 1% of palmitoyl tripeptide-5 were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0092] Example 4

[0093] 92.5% of spirulina extract, 7% of S-configuration hydroxypropyl tetrahydropyrantriol, and 0.5% of palmitoyl tripeptide-5 were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0094] Example 5

[0095] 96.5% of spirulina extract, 3.45% of S-configuration hydroxypropyl tetrahydropyrantriol, and 0.05% of palmitoyl tripeptide-5 were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0096] Example 6

[0097] Take 89.95% of spirulina extract, 10% of S-configuration hydroxypropyl tetrahydropyrantriol, and 0.05% of palmitoyl tripeptide-5 by mass, stir and mix them evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0098] Comparative Example 1

[0099] Take 10% S-configuration hydroxypropyl tetrahydropyrantriol and 95% purified water by mass, stir and mix them evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0100] Comparative Example 2

[0101] Take 1% palmitoyl tripeptide-5 and 99% purified water by mass, stir and mix them evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0102] Comparative Example 3

[0103] 95% of spirulina extract and 5% of purified water are taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0104] Comparative Example 4

[0105] Take 4.9% S-configuration hydroxypropyl tetrahydropyrantriol, 0.1% palmitoyl tripeptide-5, and 95% purified water by mass, stir and mix them evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0106] Comparative Example 5

[0107] 95% of spirulina extract, 4.9% of S-configuration hydroxypropyl tetrahydropyrantriol and 0.1% of purified water were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0108] Comparative Example 6

[0109] 95% of spirulina extract, 0.1% of palmitoyl tripeptide-5, and 4.9% of purified water were taken by mass, and stirred and mixed evenly at room temperature to obtain a whitening and anti-wrinkle composition.

[0110] Comparative Example 7

[0111] Take tranexamic acid as Comparative Example 7.

[0112] The whitening and anti-wrinkle compositions of Examples 1-6 and Comparative Examples 1-6 were dissolved in purified water to prepare a whitening and anti-wrinkle composition solution having a concentration of 0.1%, wherein the contents of the active ingredients of Examples 1-6 and Comparative Examples 1-6 are shown in Table 2 below:

[0113] Table 2

[0114]

[0115] Performance Testing

[0116] 1. Tyrosinase activity inhibition experiment

[0117] Refer to "T / SHRH 015-2018 Cosmetics-Tyrosinase Inhibition Test Method" and optimize it. Accurately pipette 50 μL of extracts of different mass concentrations, 50 μL of tyrosinase solution (50 U / mL), and 50 μL of phosphate buffer solution with a pH of 6.8, and let it stand at room temperature and away from light for 30 minutes; add 100 μL of 0.015 mol / L L-DOPA solution, mix well and react at room temperature for 10 minutes, and then measure the absorbance at 475 nm with an enzyme reader. Perform the experiment three times in parallel and take the average value. The experimental results are shown in the table. Figure 1 shown.

[0118] Depend on Figure 1 It can be seen that the whitening and anti-wrinkle compositions of Examples 1-6 of the present invention have excellent tyrosinase activity inhibition effects compared with Comparative Examples 1-6.

[0119] 2. In vitro antioxidant activity test

[0120] 100 μL of sample solution with different concentrations and 100 μL of DPPH (1 mmol / L) solution were placed in a 96-well ELISA plate. After adding the sample, the plate was shaken for 30 seconds and allowed to stand at room temperature for 2 hours. The absorbance value (Ap) was measured at 37% and 517 nm wavelength. At the same time, the blank absorbance value (Ac) of the sample without DPPH and the absorbance value (Amax) of the sample with DPPH but without sample (100 μL buffer solution was used instead of sample) were measured. The experiment was repeated 3 times, and the free radical scavenging rate was calculated according to the formula DPPH scavenging rate = l-(Ap-Ac) / Amax. The experimental results are shown in Figure 2 shown.

[0121] Depend on Figure 2 It can be seen that the whitening and anti-wrinkle compositions of Examples 1-6 of the present invention have excellent antioxidant effects compared with Comparative Examples 1-6.

[0122] 3. Comparison of the effects of the whitening and anti-wrinkle composition of Example 1 and tranexamic acid

[0123] The whitening and anti-wrinkle composition of Example 1 with different mass concentrations (0.1%, 0.3%, 0.5%, 0.7%, 0.9%) was taken as an experimental example. The tranexamic acid of Comparative Example 7 with different mass concentrations (0.1%, 0.3%, 0.5%, 0.7%, 0.9%) was taken as a control example to perform the above tyrosinase inhibition test and DPPH clearance test. The test results are shown in Figure 3 , Figure 4 shown.

[0124] Depend on Figure 3 and Figure 4It can be seen that the results of the tyrosinase activity inhibition test and the DPPH elimination rate test show that the whitening and anti-wrinkle composition has obvious antioxidant and tyrosinase activity inhibition effects, can achieve whitening and anti-wrinkle effects, and has better whitening and anti-wrinkle effects than tranexamic acid.

[0125] Application Examples

[0126] The whitening and anti-wrinkle composition of Example 1 was used to prepare a facial cream according to the formula of the facial cream described in Table 3. The steps for preparing the facial cream are as follows:

[0127] Add behenyl alcohol, dimethicone, caprylic / capric triglyceride, PEG-100 stearate and glyceryl stearate in phase B into the clean oil phase pot in sequence and heat to 80°C, stir and dissolve evenly, and keep warm for 10 minutes;

[0128] Add purified water, ammonium acryloyldimethyltaurate / VP copolymer, butanediol and glycerin in phase A into a clean emulsifying pot in sequence, homogenize at high speed for 3 minutes, heat to 80°C, keep warm for 15 minutes, and stir thoroughly until completely dissolved;

[0129] Pump phase A into the emulsifier, homogenize at high speed for 5 minutes, and continue stirring for 8 minutes;

[0130] Cool the cooling water to 45°C and add phenoxyethanol, hexylene glycol and ethylhexylglycerin in phase C in sequence;

[0131] Finally, add the whitening and anti-wrinkle composition in phase D;

[0132] After fully stirring, cool to 38°C, filter the material, and obtain a facial cream sample after passing inspection.

[0133] Table 3

[0134]

[0135] Performance Testing

[0136] 1. Evaluation of anti-wrinkle efficacy

[0137] According to the prepared facial cream samples, the anti-wrinkle efficacy of the product was evaluated before and after use.

[0138] Volunteers: Healthy Chinese female subjects aged between 30 and 55 years old.

[0139] The cheek skin is red; the basic value of the transepidermal water loss (TEWL) of the cheek area should be >10g / m when measured by the transepidermal water loss tester during the inclusion and exclusion screening and baseline value (D0) measurement. 2 / h; lack of elasticity and sagging facial skin; those who need anti-wrinkle treatment and have visible forehead wrinkles and nasolabial folds on their facial skin; not using facial products with the same effects as the test product, such as repairing, firming and anti-wrinkle products, in the month before the trial; willing not to use cosmetics, health products, drugs or other types of products with the same effects as the test product, such as repairing, firming and anti-wrinkle products, except for the test product during the trial period.

[0140] Application area: The entire face area

[0141] How to use: Take an amount the size of a pearl and apply evenly on five places on the face (forehead, bridge of nose, sides of cheeks, chin). Use your fingertips to apply evenly from the sides of the nose to the base of the ears, from the inside to the outside, lifting and tightening the contours in turn. Preheat your hands and press gently to allow it to be fully absorbed.

[0142] Test cycle: Before product use (week 0) and 4 weeks after continuous use (D28), facial skin parameters were collected using a non-invasive skin testing instrument and full-face photos were taken.

[0143] Test parameters: water content of skin stratum corneum, transepidermal water loss (TEWL), skin elasticity, skin firmness (F4), nasolabial folds area and area ratio, forehead wrinkles area and area ratio.

[0144] SPSS 21.0 statistical software was used for data analysis. Repeated measures analysis of variance was used to analyze the differences between the baseline values ​​(D0) and the follow-up values ​​(D28) of each parameter. At the D28 follow-up, the binomial test was used to analyze the differences in the self-assessment questionnaire of the subjects. Statistically significant differences were marked with p<0.05*, p<0.01**, and p<0.001***.

[0145] The moisture content of the skin stratum corneum is measured by Coreometer, CM825. The larger the value, the higher the moisture content of the skin stratum corneum. The more the moisture content of the skin stratum corneum increases compared with the baseline value (D0) at different follow-up time points, the better the moisturizing effect of the product.

[0146] Trans-epidermal water loss (TEWL) is measured by Tewameter, TM300, and is an important parameter that reflects skin water loss and barrier function. The lower the measured value, the better the skin barrier function.

[0147] Skin elasticity is measured by Cutometer, MPAS80, and its parameter R2 (ratio of rebound without negative pressure to maximum stretch with negative pressure) is used as the basis for judging skin elasticity. The closer the value is to 1, the better the skin elasticity.

[0148] Skin firmness (F4) is measured by Culometer, MPA580. F4 is the area parameter obtained after 10 cycles in the test mode. It represents the degree to which the skin is adsorbed and stretched. The smaller the value, the stronger the skin's ability to resist deformation, that is, the better the skin firmness.

[0149] The nasolabial fold parameters are obtained by collecting facial images with Visia-CR and then using Image-Pro-Plus image analysis software to analyze the nasolabial folds under the Siandard2 light source. The wrinkle area and wrinkle area ratio are the parameters that can better reflect the degree of skin wrinkles. The smaller the values ​​of these parameters, the better the nasolabial fold condition.

[0150] Forehead wrinkle parameters are obtained by collecting facial images with Visia-CR and then using Iage-Pro-Plus image analysis software to analyze forehead wrinkles under Standard2 light source. Wrinkle area and wrinkle area ratio are parameters that can better reflect the degree of skin wrinkles. The smaller the values ​​of these parameters, the better the forehead wrinkle condition.

[0151] After using the test product, the test results of the stratum corneum water content of the cheek skin were: compared with the baseline value (D0), the stratum corneum water content of the cheek skin increased by 43% at the D28 visit. After repeated measures variance analysis, compared with D0, the stratum corneum water content of the cheek skin at the D28 visit showed a significant difference (p<0.001).

[0152] After using the test product, the test results of cheek skin transepidermal water loss were: compared with the baseline value (D0), the cheek skin transepidermal water loss rate decreased by 10.34% at the D28 visit. After repeated measures analysis of variance, compared with D0, the cheek skin transepidermal water loss rate at the D28 visit showed a significant difference (p<0.001).

[0153] After using the test product, the test results of cheek skin elasticity (R2) were: compared with the baseline value (D0), cheek skin elasticity (R2) increased by 12.68% at the D28 visit. After repeated measures variance analysis, the cheek skin elasticity (R2) at the D28 visit showed a significant difference compared with D0 (p<0.001).

[0154] After using the test product, the test results of cheek skin firmness (F4) were: compared with the baseline value (D0), the cheek skin firmness (F4) decreased by 10.19% at the D28 visit. After repeated measures analysis of variance, the cheek skin firmness (F4) at the D28 visit showed a significant difference compared with D0 (p<0.001).

[0155] After using the test product, the test results of the nasolabial fold area were: compared with the baseline value (D0), the nasolabial fold area decreased by 27.20% at the fourth visit on D28. After repeated measures variance analysis, the nasolabial fold area at the D28 visit showed a significant difference compared with D0 (p<0.05).

[0156] After using the test product, the test results of forehead wrinkle area were: compared with the baseline value (D0), the forehead wrinkle area decreased by 18.00% at the D28 visit. After repeated measures variance analysis, compared with D0, the forehead wrinkle area at the D28 visit showed a significant difference (p<0.01).

[0157] After using the test product, the test results of the forehead wrinkle area ratio were: compared with the baseline value (D0), the forehead wrinkle area ratio decreased by 14.95% at the fourth visit on D28. After repeated measures variance analysis, the forehead wrinkle area ratio at the D28 visit showed a significant difference compared with D0 (p<0.01).

[0158] 2. Evaluation Questionnaire

[0159] A total of 30 subjects completed the questionnaire on the experience and effect of the test product during the D28 follow-up visit. The questionnaire on the experience and effect of the test product was given by the subjects in combination with their own experience and reference to the scoring criteria. The scoring criteria used a 1-7 point system (1 point means that the described experience or the effect of the test product is very unacceptable; 7 points means that the described experience or the effect of the test product is very acceptable; 5 points means that the described experience or the effect of the test product is somewhat acceptable, and the larger the score, the more certain the described experience or the effect of the test product is). The self-evaluation questionnaire on facial skin symptoms during the use of the test product was answered by the subjects in combination with their own conditions to determine whether the test product caused the corresponding skin symptoms on the facial skin. The results are shown in Table 4.

[0160] Table 4

[0161]

[0162] As can be seen from Table 4, at the D28 follow-up visit, 100.00% of the subjects felt that the test product had good ductility, was easy to spread, easy to absorb, non-sticky, no mud-rubbing feeling, gentle and non-irritating, and felt that the facial skin condition was more stable, the facial forehead wrinkles were faded, the facial skin was firmer, and the facial skin was plump and full; 96.67% of the subjects felt that the test product was not greasy or thick, and felt that the facial skin was softer, the facial skin pores were more delicate, the facial skin was more hydrated, and the facial skin elasticity was increased; 93.33% of the subjects felt that the facial skin was more lustrous and the facial nasolabial folds were faded (p<0.001).

[0163] It should be noted that, although the technical solution of the present invention is introduced with specific examples, those skilled in the art will appreciate that the present invention should not be limited thereto.

[0164] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used herein is intended to best explain the principles of the embodiments, practical applications, or technical improvements in the market, or to enable other persons of ordinary skill in the art to understand the embodiments disclosed herein.

[0165] Information on the deposit of biological materials

[0166] Deposit number: CCTCC NO.: M 2017772

[0167] Classification and naming: Spirulina platensis SCSIO-44012-H11

[0168] Date of deposit: December 8, 2017

[0169] Depository: CCTCC-China Center for Type Culture Collection

[0170] Address of the depository: Wuhan City, Hubei Province.

Claims

1. A whitening and anti-wrinkle composition, characterized in that: Taking the total mass of the whitening and anti-wrinkle composition as 100%, It includes the following components: Spirulina extract: 80-98%; Hydroxypropyl tetrahydropyrantriol: 0.05-10%; Small molecule peptides: 0.01~5%.

2. The whitening and anti-wrinkle composition according to claim 1, characterized in that: The spirulina extract is derived from Spirulina species, and the preservation number of the Spirulina species is CCTCC NO.: M 2017772.

3. The whitening and anti-wrinkle composition according to claim 2, characterized in that: Extracting the Spirulina species using a deep eutectic solvent to obtain a Spirulina extract; Preferably, the low eutectic solvent comprises a hydrogen bond acceptor and a hydrogen bond donor; wherein the hydrogen bond acceptor comprises a quaternary ammonium salt compound, and the hydrogen bond donor comprises a carboxylic acid compound and / or a polyol; More preferably, the extraction temperature is 55-70° C., and the extraction time is 0.5-3 hours.

4. The whitening and anti-wrinkle composition according to claim 3, characterized in that: The hydrogen bond acceptor includes choline chloride, and the hydrogen bond donor includes one or a combination of two or more of oxalic acid, malic acid, ethylene glycol, and citric acid.

5. The whitening and anti-wrinkle composition according to claim 3 or 4, characterized in that: The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:0.5-3.

6. The whitening and anti-wrinkle composition according to any one of claims 1 to 5, characterized in that: The hydroxypropyl tetrahydropyrantriol includes S-configuration hydroxypropyl tetrahydropyrantriol and / or R-configuration hydroxypropyl tetrahydropyrantriol.

7. The whitening and anti-wrinkle composition according to any one of claims 1 to 6, characterized in that: The small molecule peptide contains 2 to 10 amino acids; preferably, the small molecule peptide includes one or a combination of two or more of palmitoyl tripeptide-5, carnosine, palmitoyl pentapeptide-3, and acetyl hexapeptide-3.

8. A method for preparing the whitening and anti-wrinkle composition according to any one of claims 1 to 7, characterized in that: The method comprises the step of mixing the various components of the whitening and anti-wrinkle composition.

9. Use of the whitening and anti-wrinkle composition according to any one of claims 1 to 7 for preparing cosmetics or skin care products; preferably, the skin care products include skin care lotions, skin care essences, face creams, sunscreens, BB creams, isolation creams or facial masks; the cosmetics include liquid foundation, concealer, blush, eye shadow or lipstick.

10. An emulsification system, characterized in that: It comprises the whitening and anti-wrinkle composition according to any one of claims 1 to 7; preferably, based on the total mass of the emulsified system, the whitening and anti-wrinkle composition is added in an amount of 0.1-5%.

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