Honeysuckle extract composition with heat-clearing and anti-inflammatory effects as well as preparation method and application thereof

Abstract

The invention discloses a honeysuckle extract composition with heat-clearing and anti-inflammatory effects as well as a preparation method and application thereof, and the composition is prepared from the following raw materials in parts by weight: 20-30 parts of a honeysuckle extract, 10-20 parts of a dendrobium officinale extract, 5-10 parts of a herba houttuyniae extract, 10-20 parts of a fresh ginger extract and 5-10 parts of an ampelopsis grossedentata leaf extract. According to the composition, various plant extracts are compounded, and active ingredients in honeysuckle, ginger and ampelopsis grossedentata leaves are extracted by a specific extraction method, so that the composition has remarkable effects of clearing heat, decreasing internal heat and resisting inflammation; the dendrobium officinale extract and the herba houttuyniae extract can promote absorption of effective components in the honeysuckle, the herba houttuyniae and the ampelopsis grossedentata leaves, so that the bioavailability of active substances in the plant extract is improved, meanwhile, polysaccharide in the dendrobium officinale extract and the herba houttuyniae extract can also be constructed into a compound with chlorogenic acid in the honeysuckle, the anti-inflammatory and heat-clearing effects are synergistically achieved, and the effect of the composition is remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of functional foods, and particularly relates to a honeysuckle extract composition with heat-clearing and anti-inflammatory effects, and a preparation method and application thereof. Background Art

[0002] Modern people's living habits and living environment change frequently, which can easily cause the accumulation of fat toxins, sugar toxins and blood stasis toxins in the body, thus turning into heat toxins. The accumulation of heat toxins can damage the body at the mildest level, or cause acute myocardial infarction, atherosclerosis, thrombotic diseases and stroke at the worst level, affecting physical and mental health. Inflammation is a defensive reaction of the living tissue of the vascular system after the body is stimulated by various damaging factors. The pathological process is the exudation, degeneration and hyperplasia of the tissues in the inflamed area, and the clinical manifestations are redness, swelling, heat, pain and dysfunction.

[0003] Due to the fast pace of life, irregular eating habits, lack of sleep and other factors in today's society, the problem of decreased body resistance is becoming more and more prominent. Although traditional antipyretic, analgesic and anti-inflammatory drugs can relieve inflammatory responses to a certain extent, their strong side effects have led to a bleak market prospect. The heat-clearing and fire-reducing products on the market are mostly made of dozens of Chinese medicines due to unreasonable compatibility of Chinese herbal medicines, which leads to mutual influence or adverse effects of functional ingredients, and the effect of clearing heat and detoxifying is not ideal.

[0004] Honeysuckle, also known as honeysuckle and double flower, has chlorogenic acid as its main ingredient. It is a major medicinal material and a traditional Chinese medicine that can be used as both food and medicine. It can be used as a food supplement or as a traditional Chinese medicine. It has the effects of clearing heat, activating blood circulation, and lowering blood lipids. Both "Compendium of Materia Medica" and "Shennong's Materia Medica" have records showing that it is cold in nature, sweet in taste, and has significant effects in clearing heat and detoxifying.

[0005] At present, the functional foods developed with honeysuckle as raw materials are mainly honeysuckle beverages, honeysuckle tea, and honeysuckle health foods. Due to the unique biological activity and aroma of honeysuckle, the market products are still mainly honeysuckle tea. Chinese patent application CN103340264A discloses a honeysuckle tea and a preparation method thereof, which is prepared from 200g honeysuckle, 50g chrysanthemum, 50g corn black mold, 30g dandelion, 20g burdock root, 20g mulberry leaf, and 10g ginseng. The invention also discloses a preparation method of honeysuckle tea, including the steps of decoction, baking, cooling, and packaging and warehousing. The honeysuckle tea prepared by the invention not only has the effect of removing heat, detoxifying, moistening the intestines and defecating, but also has the effect of lowering blood sugar. However, the honeysuckle tea prepared by this method is difficult to completely dissolve the active ingredient chlorogenic acid in honeysuckle when soaked and eaten. Chinese patent application CN117859856A discloses a honeysuckle concentrated powder solid beverage and a preparation method thereof. The invention uses steam explosion to assist ultrasonic extraction to prepare honeysuckle concentrated powder, which greatly increases the content of chlorogenic acid. In the solid beverage, honeysuckle is the main drug, lily and chrysanthemum are the ministerial drugs, figs, wolfberries, and mulberry leaves are the auxiliary drugs, and tangerine peel, green fruit, and mint are the guiding drugs. The main and subordinate drugs of each party are orderly and coordinated with each other, so that the prepared solid beverage has the aroma of honeysuckle, and also has the effects of clearing the throat and moistening the lungs, clearing away heat and detoxifying. While maintaining its own raw material characteristics and flavor, it also has the advantage of good dissolution. However, the invention uses steam explosion when preparing honeysuckle concentrated powder, and its excessively high temperature may cause the degradation of the effective ingredients, thereby affecting the stability and activity of chlorogenic acid, and further affecting the heat-clearing and detoxifying effect of the solid beverage.

[0006] Therefore, it is of great significance to develop a honeysuckle extract composition with heat-clearing and anti-inflammatory effects in the art. Summary of the invention

[0007] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a honeysuckle extract composition with heat-clearing and anti-inflammatory effects, as well as a preparation method and application thereof. The composition is compounded with honeysuckle extract, Dendrobium officinale extract, Houttuynia cordata extract, ginger extract, and Ampelopsis grossedentata leaf extract. The raw materials used are all natural plant raw materials with a wide range of sources, safe and healthy. Through the mutual synergistic effect between the raw materials, the composition has excellent anti-inflammatory, heat-clearing and fire-reducing effects.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0010] 20-30 parts of honeysuckle extract, 10-20 parts of dendrobium officinale extract, 5-10 parts of houttuynia cordata extract, 10-20 parts of ginger extract, and 5-10 parts of ampelopsis grossedentata leaf extract.

[0011] Preferably, a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following raw materials by weight:

[0012] 22-30 parts of honeysuckle extract, 10-15 parts of dendrobium officinale extract, 7-10 parts of houttuynia cordata extract, 15-20 parts of ginger extract, and 5-8 parts of ampelopsis grossedentata leaf extract.

[0013] Honeysuckle, also known as honeysuckle, contains a variety of plant active ingredients that are beneficial to the human body, such as organic acids, flavonoids, volatile oils, cyclopentane glycosides, etc. Among them, organic acid components can be separated into chlorogenic acid and isochlorogenic acid with heat-clearing, detoxifying and antibacterial effects. Flavonoid components, such as luteolin and honeysuckle glycosides, are often used as the main active substances with antiviral, anti-tumor and antioxidant activities. Volatile oil components have antibacterial, anti-inflammatory and hemostatic effects. Cyclopentane glycosides play a role in protecting the liver and gallbladder.

[0014] Dendrobium officinale, also known as black knot grass, is a perennial herb that likes shade and moisture. It belongs to the genus Dendrobium of the Orchidaceae family. It was listed as one of China's medicinal and edible foods in 2021. It contains polysaccharides, polyphenols, amino acids, alkaloids, vitamins and other active ingredients. Among them, polysaccharides are the main physiologically active ingredients of Dendrobium officinale, which has multiple physiological activities such as antioxidant, immunomodulatory, anti-tumor, lipid-lowering and blood sugar-lowering.

[0015] Houttuynia cordata is a perennial herb of the genus Houttuynia of the family Herba Lycopodii, also known as Houttuynia cordata, Dog Heart Grass, and Houttuynia cordata. Houttuynia cordata contains a variety of ingredients, among which volatile oils are the main ingredients. The main active ingredients of volatile oils are decanoyl acetaldehyde, lauryl aldehyde, a-pinene, linalool, methyl n-nonyl ketone, camphene, myrcene, limonene, bornol acetate and caryophyllene. At the same time, Houttuynia cordata also contains flavonoids such as quercetin, quercetin, rutin, water-soluble polysaccharides such as fructose and glucose, and alkaloids, etc., which have anti-inflammatory, anti-tumor, antiviral, antibacterial, immunomodulatory and immunity enhancement effects.

[0016] Ginger is a perennial herbaceous plant of the ginger family. It has a strong spicy flavor and is a popular spicy vegetable and condiment. It is one of the health-care vegetables that can be used as both medicine and food in my country, and it is also a spice that people often eat. The active ingredient in ginger is mainly gingerol, which includes 6-gingerol, 6-shogaol, 6-gingerdione, 8-gingerol, 8-shogaol, 10-gingerol and 10-shogaol, with 6-gingerol being the main one. It has anti-cancer, antioxidant, antibacterial, antipyretic and anti-inflammatory, liver and kidney protection, central nervous system protection, anti-allergic and anti-gastric acid activities.

[0017] The content of flavonoids in the leaves of Agave glabra is as high as 43.4-45.5%, and it is rich in dihydromyricetin, quercetin, Agave glabra, kaempferol and other ingredients. Dihydromyricetin and myricetin are the most abundant and biologically active flavonoids in the leaves of Agave glabra, and they have multiple health functions such as antibacterial, anti-inflammatory, antioxidant, anti-cancer, lipid-lowering and blood pressure-lowering.

[0018] Preferably, the method for preparing the honeysuckle extract comprises the following steps:

[0019] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution; then add the dihydroquercetin solution to the γ-cyclodextrin solution, stir at a constant temperature to obtain a mixed solution; add honeysuckle pollen to the ethanol-water solution, soak at room temperature, add 70-80°C hot water after the soaking, then add tea theanine and the mixed solution, extract under reduced pressure under ultrasonic conditions, filter after the extraction, and perform reduced pressure rotary evaporation on the filtrate to obtain a honeysuckle extract.

[0020] In the present invention, the dihydroquercetin solution is first compounded with γ-cyclodextrin to form an inclusion solution, and then the inclusion solution is added during the extraction process of honeysuckle. The γ-cyclodextrin in the inclusion solution can improve the solubility of chlorogenic acid, increase the extraction rate and stability of chlorogenic acid, and dihydroquercetin can promote the body's absorption of chlorogenic acid, increase the bioavailability of chlorogenic acid, and synergize with the active substances in honeysuckle to play a heat-clearing and anti-inflammatory effect. At the same time, tea theanine is added during the extraction process, and the carboxyl group in theanine combines with the aromatic ring in the chlorogenic acid, which can synergistically improve the heat-clearing and anti-inflammatory effect of chlorogenic acid in the honeysuckle extract.

[0021] Preferably, the mass concentration of the dihydroquercetin solution is 0.5-1.5%, the mass concentration of the γ-cyclodextrin solution is 4-6%, the mass ratio of the dihydroquercetin solution and the γ-cyclodextrin solution is 20-30:40-60, the temperature of the constant temperature stirring is 38-45°C, and the time is 5-8h; the mass concentration of ethanol in the ethanol-water solution is 70-80%, the mass ratio of the honeysuckle pollen, ethanol-water solution, hot water, tea theanine, and mixed solution is 40-50:100-140:900-1200:0.5-1:60-90, the immersion time is 30-40min, the power of the ultrasound is 200-300W, the pressure of the reduced pressure extraction is 20-30kpa, and the time is 10-20min.

[0022] Preferably, the method for preparing ginger comprises the following steps:

[0023] Fresh ginger is washed, crushed at low temperature and then dried to obtain ginger powder; the ginger powder is then added into water, followed by cellulase and pectinase for enzymolysis, after which the enzyme is inactivated, ethanol and citric acid are added for extraction, after which the extraction is completed, the ginger is filtered and the filtrate is evaporated under reduced pressure to obtain ginger extract.

[0024] In the present invention, ginger is crushed and then dried and heat-treated, so that gingerol in the ginger can be converted into shogaol, which has better biological activity than gingerol. Subsequently, the ginger powder is enzymatically hydrolyzed and then extracted with ethanol, so that a large amount of active substances such as shogaol and flavonoids in the ginger can be extracted. Shogaol has strong antioxidant and anti-inflammatory effects. At the same time, shogaol also has a certain inhibitory effect on efflux transporters, which can promote the absorption of active ingredients in the composition and improve the bioavailability of the effective ingredients in the composition.

[0025] Preferably, the temperature of the low-temperature crushing is -50°C to -30°C, the temperature of the drying is 80-90°C, and the time is 3-5h; the mass ratio of the ginger powder, water, cellulase, pectinase, ethanol, and citric acid is 30-40:300-400:0.6-0.9:0.3-0.5:500-700:1-2, the temperature of the extraction is 60-70°C, the time is 1-2h, and the pressure of the reduced pressure evaporation is 20-30kpa.

[0026] Preferably, the method for preparing the Ampelopsis grossedentata leaf extract comprises the following steps:

[0027] The leaves of Agave sphenanthera were added into an ethanol aqueous solution and immersed for 30-40 minutes, and then vitamin C was added and ultrasonic extraction was performed. After the extraction was completed, the extract was filtered and the filtrate was evaporated under reduced pressure to obtain the Agave sphenanthera leaf extract.

[0028] In the present invention, the effective components in the leaves of Ampelopsis grossularis are extracted by ethanol-water solvent, and ultrasonic treatment is performed. Meanwhile, vitamin C is added in the extraction process to prevent the effective substances such as flavonoid compounds from being oxidized and destroyed, thereby improving the activity of dihydromyricetin in the leaves of Ampelopsis grossularis.

[0029] Preferably, the mass fraction of ethanol in the ethanol aqueous solution is 40-50%, the mass ratio of the Ampelopsis grossedentata leaves, the ethanol aqueous solution and vitamin C is 40-50:800-1000:0.5-1, the power of the ultrasonic extraction is 150-200, the temperature is 55-65°C and the time is 30-40min.

[0030] The present invention also protects a method for preparing the honeysuckle extract composition having heat-clearing and anti-inflammatory effects as described above, comprising the following steps:

[0031] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0032] The present invention also protects the use of the honeysuckle extract composition having heat-clearing and anti-inflammatory effects as described above in the preparation of anti-inflammatory and fire-reducing medicines or foods.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The honeysuckle extract composition with heat-clearing and anti-inflammatory effects provided by the present invention is prepared by compounding various plant extracts and extracting active ingredients from honeysuckle, ginger and Agave ulmoides leaves by a specific extraction method, so that the composition has significant heat-clearing, fire-reducing and anti-inflammatory effects. The ginger extract mainly contains shogaol, which can promote the absorption of effective ingredients in honeysuckle, Houttuynia cordata and Agave ulmoides leaves, thereby improving the bioavailability of active substances in the plant extracts. At the same time, the polysaccharides in the extracts of Dendrobium officinale and Houttuynia cordata can also form a complex with the chlorogenic acid in honeysuckle, synergistically exerting anti-inflammatory and heat-clearing effects, thereby significantly improving the efficacy of the composition.

[0035] (2) The honeysuckle extract composition with heat-clearing and anti-inflammatory effects provided by the present invention comprises the following steps: firstly, γ-cyclodextrin is reacted with dihydroquercetin to form an inclusion compound, thereby improving the water solubility of dihydroquercetin; then, the chlorogenic acid and other active substances in the honeysuckle are extracted by combining the reduced pressure internal boiling method with the ultrasonic method; the chlorogenic acid in the honeysuckle can be extracted at a lower temperature and in a shorter time; meanwhile, tea theanine and the mixed solution are added during the extraction process; the dihydroquercetin inclusion compound in the mixed solution can not only improve the extraction rate of chlorogenic acid during the extraction process, but also can be extracted together with the tea theanine. The high stability of chlorogenic acid can reduce the decrease in the activity of effective substances in honeysuckle during the extraction process. Theanine can also synergistically improve the heat-clearing and anti-inflammatory effects of chlorogenic acid in honeysuckle extract. The inventors of the present application unexpectedly discovered that by first forming an inclusion complex with γ-cyclodextrin and dihydroquercetin and then adding it to the honeysuckle extraction process, the extraction rate and stability of chlorogenic acid in the honeysuckle extract can be effectively improved, so that the obtained honeysuckle extract has a stronger heat-clearing and anti-inflammatory effect. At the same time, the dihydroquercetin inclusion complex can also promote the absorption of active substances in the composition, synergize with the active substances in honeysuckle to fight inflammation, and improve the bioavailability of the composition.

[0036] (3) The honeysuckle extract composition with heat-clearing and anti-inflammatory effects provided by the present invention comprises ginger extract, which is used to extract gingerol and flavonoids in ginger by an enzymatic hydrolysis-assisted method. Flavonoids have the effects of anti-oxidation and anti-aging. Gingerol can promote the absorption of effective ingredients in the composition and improve the bioavailability of active substances. The added Agave ulmoides leaves are used to fully dissolve the flavonoid compounds in the Agave ulmoides leaves by ultrasound-assisted ethanol aqueous solution as the extraction solution. Vitamin C is added during the extraction process to prevent effective substances such as flavonoid compounds from being oxidized and destroyed, thereby improving the activity of effective substances in the Agave ulmoides leaf extract. The honeysuckle extract, Dendrobium officinale extract, Houttuynia cordata extract, ginger extract and Agave ulmoides leaf extract are compounded, and the components act synergistically to make the composition have excellent anti-inflammatory and heat-clearing effects. DETAILED DESCRIPTION

[0037] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] The Dendrobium officinale extract and Houttuynia cordata extract were purchased from Xi'an Ruierli Bioengineering Co., Ltd., both of which are water extracts with a specification of 10:1.

[0039] Example 1

[0040] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0041] 25 parts of honeysuckle extract, 15 parts of dendrobium officinale extract, 8 parts of houttuynia cordata extract, 15 parts of ginger extract, and 7 parts of ampelopsis grossedentata leaf extract.

[0042] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0043] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0044] The preparation method of ginger comprises the following steps:

[0045] Wash fresh ginger, crush it at -40°C, and then dry it at 85°C for 4 hours to obtain ginger powder; then add 35g of ginger powder to 350g of water, followed by adding 0.8g of cellulase and 0.4g of pectinase, and perform enzymolysis at 45°C for 40min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 600g of ethanol and 1.5g of citric acid, extract at 65°C for 1.5h, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain ginger extract.

[0046] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0047] 45 g of Agave sphenanthera leaves were added into 900 g of ethanol aqueous solution (the mass fraction of ethanol was 45%), and soaked for 35 min. Then, 0.8 g of vitamin C was added, and the mixture was extracted for 35 min at an ultrasonic power of 180 W and a temperature of 60°C. After the extraction was completed, the mixture was filtered and the filtrate was evaporated under reduced pressure at 25 kPa to obtain the Agave sphenanthera leaf extract.

[0048] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0049] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0050] Example 2

[0051] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0052] 20 parts of honeysuckle extract, 10 parts of dendrobium officinale extract, 5 parts of houttuynia cordata extract, 10 parts of ginger extract, and 5 parts of ampelopsis grossedentata leaf extract.

[0053] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0054] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 0.5%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 4%; then add 30g of dihydroquercetin solution to 60g of γ-cyclodextrin solution, stir at a constant temperature of 38°C for 8h to obtain a mixed solution; add 40g of honeysuckle pollen to 100g of ethanol aqueous solution (the mass concentration of ethanol is 80%), soak for 30min at room temperature, add 900g of hot water at 80°C after the soaking, then add 0.5g of tea theanine and 90g of the mixed solution, extract under reduced pressure for 10min at an ultrasonic power of 200W and a pressure of 20kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 20kpa to obtain a honeysuckle extract.

[0055] The preparation method of ginger comprises the following steps:

[0056] Wash fresh ginger, crush it at -50°C, and then dry it at 80°C for 5 hours to obtain ginger powder; then add 30g of ginger powder to 300g of water, followed by adding 0.6g of cellulase and 0.3g of pectinase, and perform enzymolysis at 40°C for 50min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 500g of ethanol and 1g of citric acid, extract at 60°C for 2h, filter after the extraction, and evaporate the filtrate under reduced pressure at 20kPa to obtain ginger extract.

[0057] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0058] 40 g of Agave sphenanthera leaves were added into 800 g of ethanol aqueous solution (the mass fraction of ethanol was 50%), and soaked for 30 min. Then, 0.5 g of vitamin C was added, and extracted for 40 min at an ultrasonic power of 150 W and a temperature of 55°C. After the extraction was completed, the extract was filtered and the filtrate was evaporated under reduced pressure at 20 kPa to obtain the Agave sphenanthera leaf extract.

[0059] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0060] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0061] Example 3

[0062] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0063] 30 parts of honeysuckle extract, 20 parts of dendrobium officinale extract, 10 parts of houttuynia cordata extract, 20 parts of ginger extract, and 10 parts of ampelopsis grossedentata leaf extract.

[0064] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0065] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1.5%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 6%; then add 20g of dihydroquercetin solution to 40g of γ-cyclodextrin solution, stir at a constant temperature of 45°C for 5h to obtain a mixed solution; add 50g of honeysuckle pollen to 140g of ethanol aqueous solution (the mass concentration of ethanol is 70%), soak at room temperature for 40min, add 1200g of hot water with a temperature of 70°C after the soaking, then add 1g of tea theanine and 60g of the mixed solution, extract under reduced pressure at an ultrasonic power of 300W and a pressure of 30kpa for 10min, filter after the extraction, and evaporate the filtrate under reduced pressure at 30kpa to obtain a honeysuckle extract.

[0066] The preparation method of ginger comprises the following steps:

[0067] Wash fresh ginger, crush it at -30°C, and then dry it at 90°C for 3 hours to obtain ginger powder; then add 40g of ginger powder to 400g of water, followed by adding 0.9g of cellulase and 0.5g of pectinase, and perform enzymolysis at 50°C for 30min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 700g of ethanol and 2g of citric acid, extract at 70°C for 1h, filter after the extraction, and evaporate the filtrate under reduced pressure at 30kPa to obtain ginger extract.

[0068] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0069] Add 50g of Agave sphenanthera leaves into 1000g of ethanol aqueous solution (the mass fraction of ethanol is 40%), soak for 40 minutes, then add 1g of vitamin C, extract for 30 minutes at an ultrasonic power of 200W and a temperature of 65°C, filter after the extraction, and evaporate the filtrate under reduced pressure at 30kPa to obtain Agave sphenanthera leaf extract.

[0070] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0071] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0072] Comparative Example 1

[0073] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0074] 25 parts of honeysuckle extract, 15 parts of dendrobium officinale extract, 8 parts of houttuynia cordata extract, 15 parts of ginger extract, and 7 parts of ampelopsis grossedentata leaf extract.

[0075] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0076] Add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35 minutes, add 1100g of hot water at 75°C after soaking, then add 0.8g of tea theanine, 0.25g of dihydroquercetin, and 2.5g of γ-cyclodextrin, and extract under reduced pressure for 15 minutes at an ultrasonic power of 250W and a pressure of 25kpa. After the extraction is completed, filter and evaporate the filtrate under reduced pressure at 25kpa to obtain the honeysuckle extract.

[0077] The preparation method of ginger comprises the following steps:

[0078] Wash fresh ginger, crush it at -40°C, and then dry it at 85°C for 4 hours to obtain ginger powder; then add 35g of ginger powder to 350g of water, followed by adding 0.8g of cellulase and 0.4g of pectinase, and perform enzymolysis at 45°C for 40min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 600g of ethanol and 1.5g of citric acid, extract at 65°C for 1.5h, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain ginger extract.

[0079] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0080] 45 g of Agave sphenanthera leaves were added into 900 g of ethanol aqueous solution (the mass fraction of ethanol was 45%), and soaked for 35 min. Then, 0.8 g of vitamin C was added, and the mixture was extracted for 35 min at an ultrasonic power of 180 W and a temperature of 60°C. After the extraction was completed, the mixture was filtered and the filtrate was evaporated under reduced pressure at 25 kPa to obtain the Agave sphenanthera leaf extract.

[0081] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0082] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0083] Compared with Example 1, in this comparative example, dihydroquercetin and γ-cyclodextrin are not included to form an inclusion complex, but the two are directly added to the honeysuckle extract.

[0084] Comparative Example 2

[0085] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0086] 25 parts of honeysuckle extract, 15 parts of dendrobium officinale extract, 15 parts of ginger extract, and 7 parts of ampelopsis grossedentata leaf extract.

[0087] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0088] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0089] The preparation method of ginger comprises the following steps:

[0090] Wash fresh ginger, crush it at -40°C, and then dry it at 85°C for 4 hours to obtain ginger powder; then add 35g of ginger powder to 350g of water, followed by adding 0.8g of cellulase and 0.4g of pectinase, and perform enzymolysis at 45°C for 40min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 600g of ethanol and 1.5g of citric acid, extract at 65°C for 1.5h, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain ginger extract.

[0091] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0092] 45 g of Agave sphenanthera leaves were added into 900 g of ethanol aqueous solution (the mass fraction of ethanol was 45%), and soaked for 35 min. Then, 0.8 g of vitamin C was added, and the mixture was extracted for 35 min at an ultrasonic power of 180 W and a temperature of 60°C. After the extraction was completed, the mixture was filtered and the filtrate was evaporated under reduced pressure at 25 kPa to obtain the Agave sphenanthera leaf extract.

[0093] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0094] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0095] Compared with Example 1, this comparative example does not add Houttuynia cordata extract.

[0096] Comparative Example 3

[0097] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0098] 25 parts of honeysuckle extract, 8 parts of houttuynia cordata extract, 15 parts of ginger extract, and 7 parts of ampelopsis grossedentata leaf extract.

[0099] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0100] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0101] The preparation method of ginger comprises the following steps:

[0102] Wash fresh ginger, crush it at -40°C, and then dry it at 85°C for 4 hours to obtain ginger powder; then add 35g of ginger powder to 350g of water, followed by adding 0.8g of cellulase and 0.4g of pectinase, and perform enzymolysis at 45°C for 40min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 600g of ethanol and 1.5g of citric acid, extract at 65°C for 1.5h, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain ginger extract.

[0103] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0104] 45 g of Agave sphenanthera leaves were added into 900 g of ethanol aqueous solution (the mass fraction of ethanol was 45%), and soaked for 35 min. Then, 0.8 g of vitamin C was added, and the mixture was extracted for 35 min at an ultrasonic power of 180 W and a temperature of 60°C. After the extraction was completed, the mixture was filtered and the filtrate was evaporated under reduced pressure at 25 kPa to obtain the Agave sphenanthera leaf extract.

[0105] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0106] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, houttuynia cordata extract, ginger extract, and ampelopsis serrulata leaf extract to obtain the product.

[0107] Compared with Example 1, the present comparative example did not add the Dendrobium officinale extract.

[0108] Comparative Example 4

[0109] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0110] 25 parts of honeysuckle extract, 15 parts of Dendrobium officinale extract, 8 parts of Houttuynia cordata extract, and 7 parts of Ampelopsis grossedentata leaf extract.

[0111] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0112] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0113] The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps:

[0114] 45 g of Agave sphenanthera leaves were added into 900 g of ethanol aqueous solution (the mass fraction of ethanol was 45%), and soaked for 35 min. Then, 0.8 g of vitamin C was added, and the mixture was extracted for 35 min at an ultrasonic power of 180 W and a temperature of 60°C. After the extraction was completed, the mixture was filtered and the filtrate was evaporated under reduced pressure at 25 kPa to obtain the Agave sphenanthera leaf extract.

[0115] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0116] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, and the ampelopsis grossedentata leaf extract to obtain the product.

[0117] Compared with Example 1, ginger extract was not added in this comparative example.

[0118] Comparative Example 5

[0119] A honeysuckle extract composition with heat-clearing and anti-inflammatory effects, comprising the following raw materials by weight:

[0120] 25 parts of honeysuckle extract, 15 parts of dendrobium officinale extract, 8 parts of houttuynia cordata extract, and 15 parts of ginger extract.

[0121] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0122] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0123] The preparation method of ginger comprises the following steps:

[0124] Wash fresh ginger, crush it at -40°C, and then dry it at 85°C for 4 hours to obtain ginger powder; then add 35g of ginger powder to 350g of water, followed by adding 0.8g of cellulase and 0.4g of pectinase, and perform enzymolysis at 45°C for 40min. After the enzymolysis is completed, inactivate the enzyme at 105°C, and then add 600g of ethanol and 1.5g of citric acid, extract at 65°C for 1.5h, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain ginger extract.

[0125] A method for preparing a honeysuckle extract composition having heat-clearing and anti-inflammatory effects comprises the following steps:

[0126] Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, and the ginger extract to obtain the product.

[0127] Compared with Example 1, the present comparative example did not add the Ampelopsis grossedentata leaf extract.

[0128] The extraction rates of the honeysuckle extracts in Examples 1-3 and Comparative Example 1 were calculated and the chlorogenic acid content was determined. The results are shown in Table 1 below:

[0129] Extraction rate = (weight of honeysuckle extract - weight of dihydroquercetin - weight of γ-cyclodextrin - weight of theanine) / weight of honeysuckle powder × 100%

[0130] Table 1 Extraction rate and chlorogenic acid content of honeysuckle extract

[0131] Group Dihydroquercetin, γ-cyclodextrin and theanine weight / g Extraction rate / % Chlorogenic acid content / % Example 1 0.25g / 2.5g / 0.8g 15.8 31.7 Example 2 0.15g / 2.4g / 0.5g 14.6 29.4 Example 3 0.3g / 2.4g / 1g 15.5 30.9 Comparative Example 1 0.25g / 2.5g / 0.8g 13.3 24.6

[0132] It can be seen from Table 1 above that the preparation method of the honeysuckle extract provided by the present invention can significantly increase the content of chlorogenic acid in the extract, make the chlorogenic acid in the honeysuckle extract have better stability, and improve the quality of the honeysuckle extract.

[0133] The antipyretic effect of the compositions prepared in Examples 1-3 and Comparative Examples 1-5 on rats with fever syndrome was measured.

[0134] The compositions prepared by Examples 1-3 and Comparative Examples 1-5 were used to conduct antipyretic experiments on rats to preliminarily investigate their heat-clearing and fire-removing effects. 80 Wistar rats (basal body temperature of 37.5±0.5°C) were selected and kept in an environment with a temperature of 24°C-26°C, a relative humidity of 40%-60%, alternating light and darkness every 12h, and free access to food and water during the feeding period. The rats were randomly divided into 10 groups: a blank group, a model group, an Example 1 group, an Example 2 group, an Example 3 group, a Comparative Example 1 group, a Comparative Example 2 group, a Comparative Example 3 group, a Comparative Example 4 group, and a Comparative Example 5 group, with 8 rats in each group. Each group of rats was fasted for 6 hours, and the blank control group was subcutaneously injected with 7.5 mL / kg of normal saline; the model group, the example 1-3 group and the comparative example 1-5 group were subcutaneously injected with 200 ng / kg of Escherichia coli endotoxin. During modeling, each group of the example 1-3 group and the comparative example 1-5 group were gavaged with 300 mg / kg (5 times the recommended amount for humans) of the composition prepared by the example 1-3 and the comparative example 1-5, respectively, at a gavage volume of 0.1 mL / 10 g. The blank control group and the model control group were gavaged with an equal volume of normal saline. The body temperature was measured 4 hours after modeling and the drug was gavaged again. The anal temperature of each group of rats was also measured 6 hours and 8 hours after modeling. The results are shown in Table 2 below:

[0135] Table 2 Changes in body temperature of rats in each group (℃)

[0136]

[0137]

[0138] Note: Compared with the blank control group, *P<0.05, **P<0.01; compared with the model control group, # P<0.05, ## P<0.01

[0139] As can be seen from Table 2 above, 4h after modeling, the rectal temperature values ​​of the model group, Examples 1-3 and Comparative Examples 1-5 groups were significantly different from those of the blank group, indicating that the modeling was successful; 6h after modeling, the rectal temperature values ​​of the Example 1-3 groups were significantly different from those of the model group; 8h after modeling, the rectal temperature values ​​of the Example 1-3 groups were extremely significantly different from those of the model group, indicating that Examples 1-3 all had good antipyretic effects, which were better than those of the Comparative Example group, and the effects of the Example 1-3 groups were higher than the sum of the effects of any two groups in the Comparative Example 1-5 groups, indicating that the compositions of the present invention have synergistic effects and can significantly improve the heat-clearing effect of the compositions.

[0140] The compositions prepared in Examples 1-3 and Comparative Examples 1-5 were subjected to an anti-inflammatory test, specifically as follows: Feeding environment: temperature 24-26°C, humidity 50%-65%, daily light exposure 12h, free access to food and water, adaptive feeding for 5d before the test. Animal grouping: 60 SPF-grade C57BL / 6J adult male mice were divided into 10 groups: model control group, positive control group (dexamethasone 3mg / kg), Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group and Comparative Example 5 group, 6 mice in each group, Example 1-3 group and Comparative Example 1-5 group were gavaged with 300mg / kg of the composition prepared in Examples 1-3 and Comparative Example 1-5, respectively, with a gavage volume of 0.1mL / 10g, and the model control group was gavaged with an equal volume of physiological saline, 1 time / d, for 7 consecutive days. 30 minutes after the last gavage, 40 μL of xylene was evenly applied on the inside and outside of the right ear of the mouse with a micropipette (20 μL on each side of the right ear) to cause swelling of the auricle. The mouse was killed 1 hour after inflammation, and the two ears of the mouse were cut off. A 6mm puncher was used to punch round ear pieces of equal area at the same part of the two ears. The mass was accurately weighed, and the swelling degree and swelling inhibition rate were calculated. Calculation formula: swelling degree (mg) = right ear mass (mg) - left ear mass (mg). Swelling inhibition rate = [(average swelling degree of the model group - average swelling degree of the drug group) / average swelling degree of the model group] × 100%, and the test results are shown in Table 3 below:

[0141] Table 3 Comparison of ear swelling in each group of mice

[0142] Number of animals Swelling degree (mg) Swelling inhibition rate (%) Model control group 6 3.78±1.34 / Positive control group 6 1.46±0.72** 61.38 Example 1 Group 6 1.71±0.79** 54.76 Example 2 Group 6 1.82±0.67** 51.85 Example 3 Group 6 1.75±0.78** 53.70 Comparative Example 1 6 2.16±0.85* 42.86 Comparative Example 2 6 2.24±0.99* 40.74 Comparative Example 3 6 2.31±0.91 38.89 Comparative Example 4 6 2.29±1.06 39.42 Comparative Example 5 6 2.33±0.87 38.36

[0143] Note: Compared with the model control group, *P<0.05, **P<0.01;

[0144] Inflammation is a defensive reaction of living tissue with a vascular system to local damage. It is a dynamic process that mobilizes cells with different functions to participate, which will cause an increase in white blood cells in the blood and tissues. The main symptoms of inflammation are redness, swelling, heat, pain and dysfunction. As can be seen from Table 3 above, compared with the model control group, the swelling of the ears of the positive control group and the Example 1-3 groups of mice was significantly reduced, and there was a very significant difference, indicating that the composition prepared by the present invention has a good anti-inflammatory effect.

[0145] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A honeysuckle extract composition having heat-clearing and anti-inflammatory effects, characterized in that: By weight, it includes the following raw materials: 20-30 parts of honeysuckle extract, 10-20 parts of dendrobium officinale extract, 5-10 parts of houttuynia cordata extract, 10-20 parts of ginger extract, and 5-10 parts of ampelopsis grossedentata leaf extract.

2. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 1, characterized in that: By weight, it includes the following raw materials: 22-30 parts of honeysuckle extract, 10-15 parts of dendrobium officinale extract, 7-10 parts of houttuynia cordata extract, 15-20 parts of ginger extract, and 5-8 parts of ampelopsis grossedentata leaf extract.

3. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 1, characterized in that: The preparation method of the honeysuckle extract comprises the following steps: Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution; then add the dihydroquercetin solution to the γ-cyclodextrin solution, stir at a constant temperature to obtain a mixed solution; add honeysuckle pollen to the ethanol-water solution, soak at room temperature, add 70-80°C hot water after the soaking, then add tea theanine and the mixed solution, extract under reduced pressure under ultrasonic conditions, filter after the extraction, and perform reduced pressure rotary evaporation on the filtrate to obtain a honeysuckle extract.

4. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 3, characterized in that: The mass concentration of the dihydroquercetin solution is 0.5-1.5%, the mass concentration of the γ-cyclodextrin solution is 4-6%, the mass ratio of the dihydroquercetin solution to the γ-cyclodextrin solution is 20-30:40-60, the temperature of the constant temperature stirring is 38-45°C, and the time is 5-8h; the mass concentration of ethanol in the ethanol-water solution is 70-80%, the mass ratio of the honeysuckle pollen, the ethanol-water solution, hot water, tea theanine, and the mixed solution is 40-50:100-140:900-1200:0.5-1:60-90, the immersion time is 30-40min, the power of the ultrasound is 200-300W, the pressure of the reduced pressure extraction is 20-30kpa, and the time is 10-20min.

5. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 1, characterized in that: The preparation method of ginger comprises the following steps: Fresh ginger is washed, crushed at low temperature and then dried to obtain ginger powder; the ginger powder is then added into water, followed by cellulase and pectinase for enzymolysis, after which the enzyme is inactivated, ethanol and citric acid are added for extraction, after which the extraction is completed, the ginger is filtered and the filtrate is evaporated under reduced pressure to obtain ginger extract.

6. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 5, characterized in that: The temperature of the low-temperature crushing is -50°C to -30°C, the temperature of the drying is 80-90°C, and the time is 3-5h; the mass ratio of the ginger powder, water, cellulase, pectinase, ethanol, and citric acid is 30-40:300-400:0.6-0.9:0.3-0.5:500-700:1-2, the temperature of the extraction is 60-70°C, the time is 1-2h, and the pressure of the reduced pressure evaporation is 20-30kpa.

7. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 1, characterized in that: The preparation method of the Ampelopsis grossedentata leaf extract comprises the following steps: The leaves of Agave sphenanthera were added into an ethanol aqueous solution and immersed for 30-40 minutes, and then vitamin C was added and ultrasonic extraction was performed. After the extraction was completed, the extract was filtered and the filtrate was evaporated under reduced pressure to obtain the Agave sphenanthera leaf extract.

8. The honeysuckle extract composition with heat-clearing and anti-inflammatory effects according to claim 7, characterized in that: The mass fraction of ethanol in the ethanol aqueous solution is 40-50%, the mass ratio of the Ampelopsis grossedentata leaves, the ethanol aqueous solution and vitamin C is 40-50:800-1000:0.5-1, the power of the ultrasonic extraction is 150-200W, the temperature is 55-65°C and the time is 30-40min.

9. A method for preparing the honeysuckle extract composition having heat-clearing and anti-inflammatory effects as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: Weigh the raw materials according to the formula, and evenly mix the honeysuckle extract, the dendrobium officinale extract, the houttuynia cordata extract, the ginger extract, and the ampelopsis grossedentata leaf extract to obtain the product.

10. Use of the honeysuckle extract composition with heat-clearing and anti-inflammatory effects as claimed in any one of claims 1 to 8 in the preparation of anti-inflammatory and fire-reducing medicines or foods.

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