Composition for skin whitening and anti-aging as well as preparation method and application thereof

Abstract

The invention discloses a composition for skin whitening and anti-aging and a preparation method and application thereof, and belongs to the technical field of compositions.The composition is prepared from collagen tripeptide, pleurotus citrinopileatus powder, osmanthus fragrans powder, muskmelon concentrated powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt. The preparation method comprises the following steps: mixing collagen tripeptide, pleurotus citrinopileatus powder, sweet-scented osmanthus powder, muskmelon concentrated powder, a grape seed extract, olive powder and pyrroloquinoline quinone disodium salt, and sieving; the application refers to application of the composition in preparation of antioxidant products. Experiments prove that the collagen tripeptide, the pleurotus citrinopileatus powder, the osmanthus fragrans powder, the muskmelon concentrated powder, the grape seed extract, the olive powder and the pyrroloquinoline quinone disodium salt are compounded, so that a synergistic interaction effect is generated in the aspects of oxidation resistance and melanin formation inhibition, and compared with the prior art, the whitening and anti-aging effects are better.

Description

Technical Field

[0001] The present invention relates to the technical field of compositions, and in particular to a composition for skin whitening and anti-aging, and a preparation method and application thereof. Background Art

[0002] The degree of skin whitening is closely related to the formation of melanin. Melanin is an important biological pigment that protects human skin from ultraviolet radiation, but it is affected by genetics, endocrine, sunlight exposure and other factors. Excessive production of melanin leads to skin pigmentation, which can cause problems such as dull skin, darkening and chloasma. Melanin is mainly divided into true melanin and pheomelanin. The biosynthesis of melanin in the body involves tyrosine-tyrosinase reaction. L-Tyrosine is oxidized to L-dopaquinone under the action of tyrosinase, and then generates dihydroxyindole through intramolecular cyclization, oxidation and isomerization. Dihydroxyindole is oxidized to true melanin under the catalysis of melanin precursor oxidase. At the same time, dopaquinone is oxidized to generate benzothiazine intermediates and further forms pheomelanin. The formation of melanin requires the participation of oxygen free radicals, and the synthesis process is affected by the rate-limiting effect of tyrosinase. Therefore, the synthesis of melanin can be inhibited by two aspects of anti-oxidation and inhibition of tyrosinase activity to play a role in whitening and anti-aging.

[0003] CN105816344B discloses a composition containing pyrroloquinoline quinone or a salt thereof and N-acetylneuraminic acid, wherein the mass ratio of pyrroloquinoline quinone or a salt thereof to N-acetylneuraminic acid is 1:1-10, and the most preferred mass ratio of pyrroloquinoline quinone or a salt thereof to N-acetylneuraminic acid is 1:5.5. The composition has a good antioxidant effect and can be used in cosmetics and foods.

[0004] CN117918517A discloses an anti-aging composition, comprising concentrated powder of Pleurotus eryngii, disodium pyrroloquinoline, rice germ powder, red grape yeast powder and fish protein powder. The invention can remove old cells from the root, provide energy for cells, and effectively promote cell growth through the synergistic effect between concentrated powder of Pleurotus eryngii, disodium pyrroloquinoline, rice germ powder, red grape yeast powder and fish protein powder, thereby achieving an anti-aging effect.

[0005] Although the above existing technologies have certain whitening and anti-aging effects, there is still room for improvement. Summary of the invention

[0006] The purpose of the present invention is to provide a composition for skin whitening and anti-aging, and its preparation method and use. The composition of the present invention has synergistic effects in both anti-oxidation and inhibition of melanin formation, and has better whitening and anti-aging effects.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions: A composition for skin whitening and anti-aging, comprising collagen tripeptide, pleurotus eryngii powder, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt.

[0008] In some embodiments, the weight ratio of the Pleurotus eryngii powder, osmanthus powder, olive powder and pyrroloquinoline quinone disodium salt is 2-10:2-10:2-10:0.02-0.4, preferably 5:6:6:0.2.

[0009] In some embodiments, the composition is composed of the following raw materials in parts by weight: 20-100 parts of collagen tripeptide, 2-10 parts of Pleurotus eryngii powder, 2-10 parts of osmanthus powder, 2-10 parts of melon concentrate powder, 0.5-5 parts of grape seed extract, 2-10 parts of olive powder and 0.02-0.4 parts of pyrroloquinoline quinone disodium salt.

[0010] In some embodiments, the composition is composed of the following raw materials in parts by weight: 30-90 parts of collagen tripeptide, 4-8 parts of Pleurotus eryngii powder, 3-9 parts of osmanthus powder, 3-9 parts of melon concentrate powder, 1-4 parts of grape seed extract, 5-7 parts of olive powder and 0.1-0.3 parts of pyrroloquinoline quinone disodium salt.

[0011] In some embodiments, the composition is composed of the following raw materials in parts by weight: 60 parts of collagen tripeptide, 5 parts of Pleurotus eryngii powder, 6 parts of osmanthus powder, 5 parts of melon concentrate powder, 2 parts of grape seed extract, 6 parts of olive powder and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0012] In a second aspect, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: mixing collagen tripeptide, Pleurotus eryngii powder, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt, and sieving.

[0013] In some embodiments, the sieving is through a 60-80 mesh sieve.

[0014] In a third aspect, the present invention provides use of the composition in preparing antioxidant functional foods.

[0015] In some specific embodiments, the functional food is in any form of granules, blocks, powder, liquid, or paste.

[0016] In a fourth aspect, the present invention provides an antioxidant functional food composition comprising the above composition.

[0017] In some specific embodiments, the antioxidant functional food further comprises auxiliary materials acceptable in health products.

[0018] The beneficial effects of the present invention are: (1) Experiments have shown that the present invention utilizes a combination of collagen tripeptide, Pleurotus eryngii powder, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt to produce a synergistic effect in terms of antioxidant properties and inhibition of melanin formation. Compared with the existing technology, the whitening and anti-aging effects are better.

[0019] (2) The present invention makes full use of the synergistic effect between the raw materials by mixing different functional raw materials. It acts on the skin simultaneously through endogenous repair (inhibiting melanin formation, promoting melanin metabolism, and supplementing collagen) and exogenous protection (resisting ultraviolet radiation and establishing a moisture barrier). The synergistic effect of multiple aspects achieves the effects of whitening and anti-aging. DETAILED DESCRIPTION

[0020] The following examples are only used to help understand the method of the present invention and its core idea. It should be noted that for those of ordinary skill in the art, without departing from the principles of the present invention, the present invention may also be subjected to several improvements and modifications, which also fall within the scope of protection of the claims of the present invention. The following description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. It will be apparent to professionals and technicians in this field that various modifications to these embodiments, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but may be applied to a wider range consistent with the principles and novel features disclosed herein. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0021] Hereinafter, examples and comparative examples of the present invention will be shown to explain the composition of the present invention in more detail, but the present invention is not limited thereto.

[0022] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are common commercial products in the technical field.

[0023] The purchase information of raw materials is shown in Table 1.

[0024] Table 1 Raw material information

[0025] Example 1 Composition for skin whitening and anti-aging The composition comprises the following raw materials by weight: 60 parts of collagen tripeptide, 5 parts of Pleurotus eryngii powder, 6 parts of osmanthus powder, 5 parts of melon concentrated powder, 2 parts of grape seed extract, 6 parts of olive powder and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0026] Preparation method: Mix the formulated amount of collagen tripeptide, Pleurotus eryngii powder, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt, and pass through an 80-mesh sieve.

[0027] Example 2 Composition for skin whitening and anti-aging The composition comprises the following raw materials by weight: 30 parts of collagen tripeptide, 4 parts of Pleurotus eryngii powder, 9 parts of osmanthus powder, 3 parts of melon concentrated powder, 4 parts of grape seed extract, 5 parts of olive powder and 0.3 parts of pyrroloquinoline quinone disodium salt.

[0028] The preparation method is the same as Example 1.

[0029] Example 3 Composition for skin whitening and anti-aging The composition comprises the following raw materials by weight: 90 parts of collagen tripeptide, 8 parts of Pleurotus eryngii powder, 3 parts of osmanthus powder, 9 parts of melon concentrated powder, 1 part of grape seed extract, 7 parts of olive powder and 0.1 part of pyrroloquinoline quinone disodium salt.

[0030] The preparation method is the same as Example 1.

[0031] Example 4 Composition for skin whitening and anti-aging The composition comprises the following raw materials by weight: 20 parts of collagen tripeptide, 2 parts of Pleurotus eryngii powder, 10 parts of osmanthus powder, 2 parts of melon concentrated powder, 5 parts of grape seed extract, 2 parts of olive powder and 0.4 parts of pyrroloquinoline quinone disodium salt.

[0032] The preparation method is the same as Example 1.

[0033] Example 5 Composition for skin whitening and anti-aging The composition comprises the following raw materials by weight: 100 parts of collagen tripeptide, 10 parts of Pleurotus eryngii powder, 2 parts of osmanthus powder, 10 parts of melon concentrated powder, 0.5 parts of grape seed extract, 10 parts of olive powder and 0.02 parts of pyrroloquinoline quinone disodium salt.

[0034] The preparation method is the same as Example 1.

[0035] Comparative Example 1 Composition for skin whitening and anti-aging The difference between this comparative example and Example 1 is that it does not contain Pleurotus eryngii powder and olive powder.

[0036] Specifically: 66 parts of collagen tripeptide, 6 parts of osmanthus powder, 10 parts of melon concentrate powder, 2 parts of grape seed extract and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0037] The preparation method is as follows: mix the formulated amount of collagen tripeptide, osmanthus powder, melon concentrated powder, grape seed extract and pyrroloquinoline quinone disodium salt, and pass through an 80-mesh sieve.

[0038] Comparative Example 2 Composition for skin whitening and anti-aging The difference between this comparative example and Example 1 is that it does not contain osmanthus powder and pyrroloquinoline quinone disodium salt.

[0039] Specifically, the composition comprises the following raw materials, by weight: 60 parts of collagen tripeptide, 11 parts of Pleurotus eryngii powder, 5 parts of melon concentrate powder, 2 parts of grape seed extract and 6.2 parts of olive powder.

[0040] Preparation method: Mix the formulated amount of collagen tripeptide, Pleurotus eryngii powder, melon concentrate powder, grape seed extract and olive powder, and pass through an 80-mesh sieve.

[0041] Comparative Example 3 Composition for skin whitening and anti-aging The difference between this comparative example and Example 1 is that the total amount of the Pleurotus eryngii powder, osmanthus powder, olive powder and pyrroloquinoline quinone disodium salt is the same, but the weight ratio is different.

[0042] Specifically, the composition comprises the following raw materials, by weight: 60 parts of collagen tripeptide, 0.2 parts of Pleurotus eryngii powder, 1 part of osmanthus powder, 5 parts of melon concentrate powder, 2 parts of grape seed extract, 12 parts of olive powder and 4 parts of pyrroloquinoline quinone disodium salt.

[0043] The preparation method is the same as that of Example 1.

[0044] Comparative Example 4 Composition for skin whitening and anti-aging The difference between this comparative example and Example 1 is that N-acetylneuraminic acid is used instead of Pleurotus eryngii powder.

[0045] Specifically: The composition comprises the following raw materials by weight: 60 parts of collagen tripeptide, 5 parts of N-acetylneuraminic acid, 6 parts of osmanthus powder, 5 parts of melon concentrated powder, 2 parts of grape seed extract, 6 parts of olive powder and 0.2 parts of pyrroloquinoline quinone disodium salt.

[0046] Preparation method: Mix the formulated amount of collagen tripeptide, N-acetylneuraminic acid, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt, and pass through an 80-mesh sieve.

[0047] 1. Antioxidant properties of the composition of the present invention Refer to T / ZHCA 502-2020 zebrafish assay for antioxidant function Principle: The level of ROS in the body is determined by the balance between the production and elimination of reactive oxygen species in the body. When the level of ROS in the body rises, it will consume antioxidant substances in the body. If the synthesis rate of antioxidant substances in the body is slow and the clearance rate of ROS by antioxidant enzymes is not fast enough, it will lead to an increase in the level of lipid and protein peroxidation, causing physiological phenomena related to peroxidation such as aging. If the test substance can accelerate the synthesis rate of antioxidant substances in the body and enhance the activity of antioxidant enzymes, it can quickly eliminate the ROS produced in the body, thereby reducing the level of lipid and protein peroxidation induced by ROS, so that the production and elimination of reactive oxygen species in the body remain in a balance that is beneficial to the health of the body. Therefore, ROS is an indicator that can more directly reflect the balance between the production and elimination of reactive oxygen species in the body. After being treated with the test substance, the reduction of ROS levels in zebrafish can also directly reflect the antioxidant capacity of the test substance.

[0048] Experimental animals: Zebrafish were raised in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO3), bred and provided by our company's fish farming center, the experimental animal use license number is: SYXK (Zhejiang) 2022-0004, and the breeding management complies with the requirements of the international AAALAC certification (certification number: 001458), and the IACUC ethics review number is: IACUC-2024-8244-01.

[0049] Test samples: compositions prepared in Examples 1-5, and compositions prepared in Comparative Examples 1-4.

[0050] Experimental method: 9hpf zebrafish embryos were randomly selected in a 12-well plate. At the same time, a normal control group, a model control group and an experimental group were set up. Ten zebrafish embryos were treated in each well, and the volume of each well was 2 mL. 2mL of deionized water was added to each well of the normal group, 1.9mL of deionized water was added to each well of the model control group, and 1.8mL of sample solution (100μg / mL) was added to the experimental group. After 2h, 100μL of LPS solution was added to each well of each group except the normal control group, so that the final concentration of LPS was 10μg / mL, and incubated at 28℃ for 24h to construct an oxidative stress model.

[0051] After 24 hours, each well of each group was washed three times with deionized water, and then 2 mL DCHF-DA (100 μg / mL) was added and incubated at 28°C in the dark for 60 minutes. Then the zebrafish embryos were washed three times with deionized water, anesthetized with tricaine, and photographed under a fluorescence microscope to calculate their fluorescence intensity.

[0052] The results are shown in Table 2.

[0053] Table 2 Antioxidant properties of the composition of the present invention (mean ± sd)

[0054] Note: Different letters in the same column indicate significant differences among the groups, P < 0.05.

[0055] The results showed that compared with the normal control group, the fluorescence intensity of zebrafish embryos in the model group was significantly enhanced (P < 0.05), indicating that the LPS-induced oxidative stress model was successfully established.

[0056] Compared with the model control group, the fluorescence intensity of zebrafish embryos in Examples 1-5 in the test group was significantly weakened (P < 0.05), indicating that in the technical solution claimed by the present invention, the composition prepared in Examples 1-5 can effectively remove excessive ROS and has a good antioxidant effect.

[0057] Compared with the model control group, the fluorescence intensity of zebrafish embryos in the comparative examples 1-4 groups in the test group was weakened to a certain extent, but there was no significant difference (P>0.05), indicating that the composition prepared in comparative examples 1-4 in the technical solution claimed by the present invention has no antioxidant effect.

[0058] By comparing Example 1 and Comparative Examples 1-2, it can be seen that in the technical scheme claimed for protection by the present invention, only the use of osmanthus powder and pyrroloquinoline quinone disodium salt or Pleurotus eryngii powder and olive powder in combination with other components cannot achieve the antioxidant effect at all. The present invention uses Pleurotus eryngii powder, osmanthus powder, olive powder and pyrroloquinoline quinone disodium salt in combination with other components, which plays a synergistic role in providing antioxidant effects.

[0059] II. Test on the inhibition of melanin production by the composition of the present invention B16 melanoma cells were used as experimental subjects, and the melanin content in the cells was detected by alkaline lysis method to study the effect of the composition of the present invention on inhibiting melanin production.

[0060] 1. Reagents, consumables and instruments 1.1 Reagents and consumables 1×PBS solution, DMEM basic medium, DMEM complete medium (DMEM+10% serum+1% double antibody), trypsin (0.25% trypsin-EDTA, Gibco 25200072), 1M NaOH (PBS solution containing 10% DMSO).

[0061] 1.2 Test samples The compositions prepared in Examples 1-5 and the compositions prepared in Comparative Examples 1-4.

[0062] 1.3 Test equipment Incubator, microplate reader and water bath 2. Melanin detection method (1) Inoculate B16 cell suspension (1×10 5 cells / well) 1 mL, 37°C, 5% CO2 incubator for 24 h; (2) For the blank control group, 1 mL of DMEM complete medium was added to each well. For the positive control group, 1 mL of 100 μg / mL kojic acid (DMEM complete medium was used as the solvent) was added to each well. For the treatment group, the test samples were added, and 3 replicate wells were set up for each group.

[0063] (3) Discard the old culture medium and add 1 mL of 50 μg / mL test sample (DMEM complete culture medium as solvent) to each well of the treatment group and culture at 37°C for 72 h; (4) Discard the old culture medium and add 1 mL of PBS to the plate and wash once; (5) Add 500 μL of trypsin to each well and place in a 37°C incubator for 5 min to allow for complete digestion. (6) Transfer the digested cells to a 1.5 mL centrifuge tube, centrifuge, remove the supernatant, resuspend with 500 μL PBS (wash once), transfer to a centrifuge tube, and 1×10 4 Centrifuge at rpm for 10 min; (7) Discard the supernatant, add 200 μL 1M NaOH, and incubate in a water bath at 80°C for 30 min until the melanin is dissolved. Transfer 100 μL of the solution to a 96-well plate and read the plate at a wavelength of 475 nm.

[0064] The formula for calculating the relative content of melanin is: relative content of melanin = absorbance value of the treatment group / absorbance value of the blank control group. The results are shown in Table 3.

[0065] Table 3 Inhibition of melanin formation by the composition of the present invention

[0066] Note: Different letters in the same column indicate significant differences among the groups, P < 0.05.

[0067] The results showed that compared with the blank control group, the compositions prepared in Examples 1-5 of the present invention had the effect of inhibiting the formation of melanin (P < 0.05), among which the effect of the compositions prepared in Examples 1-3 on inhibiting the formation of melanin was better than that of the positive drug (P < 0.05), and the effect of the compositions prepared in Examples 4-5 on inhibiting the formation of melanin was basically the same as that of the positive drug (P > 0.05). Therefore, the compositions prepared in Examples 1-3 had a better effect of inhibiting the formation of melanin.

[0068] Compared with the positive control group, the effect of inhibiting melanin formation of the composition prepared in comparative examples 1-4 is significantly inferior to that of the positive drug (P < 0.05). This indicates that the effect of inhibiting melanin formation of the composition prepared in comparative examples 1-4 is significantly inferior to that of the composition prepared in examples 1-5.

[0069] By comparing Example 1 and Comparative Examples 1-2, it can be seen that in the technical scheme claimed for protection by the present invention, only the combination of osmanthus powder and disodium pyrroloquinoline quinone or Pleurotus eryngii powder and olive powder with other components cannot achieve the effect of inhibiting melanin better than positive drugs. The present invention uses Pleurotus eryngii powder, osmanthus powder, olive powder and disodium pyrroloquinoline quinone in combination with other components, which plays a synergistic role in providing inhibition of melanin formation.

[0070] The above is a further description of the present invention in conjunction with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

Claims

1. A composition for skin whitening and anti-aging, characterized in that: It is composed of collagen tripeptide, Pleurotus eryngii powder, osmanthus powder, melon concentrate powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt.

2. The composition according to claim 1, characterized in that The weight ratio of the Pleurotus eryngii powder, osmanthus powder, olive powder and pyrroloquinoline quinone disodium salt is 2-10:2-10:2-10:0.02-0.

4.

3. The composition according to claim 2, characterized in that The invention is composed of the following raw materials in parts by weight: 20-100 parts of collagen tripeptide, 2-10 parts of pleurotus eryngii powder, 2-10 parts of osmanthus powder, 2-10 parts of melon concentrated powder, 0.5-5 parts of grape seed extract, 2-10 parts of olive powder and 0.02-0.4 parts of disodium pyrroloquinoline quinone.

4. The composition according to claim 3, characterized in that The invention is composed of the following raw materials in parts by weight: 30-90 parts of collagen tripeptide, 4-8 parts of pleurotus eryngii powder, 3-9 parts of osmanthus powder, 3-9 parts of melon concentrated powder, 1-4 parts of grape seed extract, 5-7 parts of olive powder and 0.1-0.3 parts of disodium pyrroloquinoline quinone.

5. The composition according to claim 1, characterized in that The weight ratio of the Pleurotus eryngii powder, osmanthus powder, olive powder and pyrroloquinoline quinone disodium salt is 5:6:6:0.

2.

6. The composition according to claim 5, characterized in that The invention is composed of the following raw materials in parts by weight: 60 parts of collagen tripeptide, 5 parts of Pleurotus eryngii powder, 6 parts of osmanthus powder, 5 parts of melon concentrated powder, 2 parts of grape seed extract, 6 parts of olive powder and 0.2 parts of pyrroloquinoline quinone disodium salt.

7. A method for preparing the composition according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: mixing collagen tripeptide, pleurotus eryngii powder, osmanthus powder, melon concentrated powder, grape seed extract, olive powder and pyrroloquinoline quinone disodium salt, and sieving.

8. Use of the composition according to any one of claims 1 to 6 or the composition prepared by the preparation method according to claim 7 in preparing antioxidant functional foods.

9. The use according to claim 8, characterized in that The functional food is in any form of granules, blocks, powder, liquid or paste.

10. An antioxidant functional food composition, characterized in that: A composition comprising the composition according to any one of claims 1 to 6 or a composition prepared by the preparation method according to claim 7.

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