Probiotic crystal ball with high viable bacteria rate and preparation method thereof

Abstract

According to the probiotic crystal ball with the high viable bacterium rate and the preparation method of the probiotic crystal ball, sea buckthron oil serves as base oil to replace normal-temperature solid fat with the high melting point, the problem that oil powder is not easy to combine is solved, homogenization can be conducted at the normal temperature in the preparation process, and the inactivation rate of probiotics is reduced; and after homogenization treatment, the problem that the pipeline of the pill dropping machine is easily blocked due to large selectable material particles is solved. In addition, a double-layer bag skin structure is adopted, the first bag skin is a crystal ball shell, the amount of glycerin in the first bag skin is a little, the shell elasticity can be kept, the situation that in the drying process, the shell is broken after being hardened, and consequently liquid leakage is caused is avoided, and the first bag skin can rapidly absorb water and soften in the swallowing process and is easy to swallow. And pectin is added into the capsule skin II, and the amount of glycerol is small, so that the capsule skin II can be quickly hardened in the drying process to form a shell with hard crystal balls, and can tolerate gastric acid in the digestive tract, thereby keeping the crystal ball form not broken and achieving the effect of protecting the probiotics to live to the intestinal tract.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotic embedding, and specifically relates to a probiotic crystal ball with a high viable bacteria rate and a preparation method thereof. Background Art

[0002] Probiotics are a type of microorganism that is beneficial to human health and plays an important role in the intestines. Probiotics can help maintain the balance of intestinal flora, enhance the function of the immune system, promote food digestion and absorption, and help prevent and relieve intestinal problems such as diarrhea. By increasing the intake of probiotics, intestinal health can be improved and overall physical health can be improved. However, probiotic products will cause loss of vitality during production, storage, and transportation. And after probiotics are ingested into the body, there will be more losses due to the interaction of food components, the strong acidic pH environment of the stomach, and the action of various digestive enzymes.

[0003] At present, in traditional process dosage forms, probiotics are mostly in the form of powder. If sea buckthorn oil and probiotic powder are added to the product at the same time, it will lead to conflicts in product properties, and the oil and powder cannot be combined together and ensure easy consumption. In addition, if turmeric powder, garlic oil and other medicinal and edible raw materials with a certain spicy taste or smell are directly added to the probiotic powder, it will cause a serious rejection of users. In order to overcome the above obstacles, the patent of this invention uses gelatin, glycerin and other raw materials to organically combine probiotics and medicinal and edible raw materials, making it easy to take, without irritating odor and taste, which not only retains the high viability of probiotics after processing in the production process, but also ensures that the probiotics can successfully pass through the gastrointestinal barrier alive.

[0004] At present, the crystal ball probiotics on the market all use high-melting-point palm oil or hydrogenated vegetable oil as the carrier of probiotic powder, which is used as the core material wrapped inside the crystal ball. The crystal ball must heat and melt the high-melting-point palm oil or hydrogenated vegetable oil, and then add the probiotic powder and stir it evenly. In order to improve the activity of the probiotics inside the crystal ball, the patent of this invention has made a process innovation, replacing the core material carrier of the crystal ball with sea buckthorn oil that is liquid at room temperature, and at the same time improving the process of ensuring the activity of the strain, to obtain a crystal ball probiotic preparation process with a high survival rate. Summary of the invention

[0005] In order to solve the above defects and shortcomings in the prior art, the present invention provides a probiotic crystal ball with a high viable bacteria rate and a preparation method thereof.

[0006] To solve the above technical problems: The first object of the present invention is to provide a probiotic crystal ball with a high viable bacteria rate, comprising a core material, a capsule skin 1 and a capsule skin 2 arranged in sequence from the inside to the outside; wherein: The core material includes probiotic powder and sea buckthorn oil as a carrier base oil; wherein the amount of probiotics can be loaded according to the actual required amount of live bacteria.

[0007] The weight proportions of the components of the capsule skin are: 20%-40% gelatin, 8%-20% glycerin, and the rest is water; preferably, the gelatin is 22%-30%, and the glycerin is 10%-18%; optimally, the gelatin is 24% and the glycerin is 16%.

[0008] The weight proportions of the components of the second capsule shell are: 20%-40% gelatin, 0.5%-10% pectin, 8%-30% glycerin, and the rest is water. Preferably, the gelatin is 25%-35%, the glycerin is 8%-15%, and the pectin is 2%-8%; the most preferred proportion is 30% gelatin, 10% glycerin, and 4% pectin.

[0009] Furthermore, the core material also includes optional materials, and the weight proportion of the optional materials is 0.1-5%.

[0010] Furthermore, the optional materials are one or a combination of Hericium edodes powder, Astragalus powder, Turmeric powder, garlic oil, sweet orange oil, and mint extract.

[0011] Furthermore, the amount of probiotic powder can be adjusted as needed. As a preferred embodiment, the weight proportions of the core material components are: 1%-10% probiotic powder, and the rest is sea buckthorn oil as the base oil; The weight proportions of the components of the capsule skin are: 20%-40% gelatin, 8%-20% glycerin, and the rest is water; preferably, the gelatin is 22%-30%, and the glycerin is 10%-18%; optimally, the gelatin is 24% and the glycerin is 16%.

[0012] The weight proportions of the components of the second capsule shell are: 20%-40% gelatin, 0.5%-10% pectin, 8%-30% glycerin, and the rest is water. Preferably, the gelatin is 25%-35%, the glycerin is 8%-15%, and the pectin is 2%-8%; the most preferred proportion is 30% gelatin, 10% glycerin, and 4% pectin.

[0013] Another object of the present invention is to provide a method for preparing probiotic crystal spheres with a high viable bacteria rate, comprising the following steps: S1. Preparation of capsule skin: 20%-40% gelatin, 8%-20% glycerin and deionized water are mixed, stirred in a gelling tank at 60-85°C for 1-3h, vacuumed and degassed until there are no bubbles, and allowed to stand for standby after breaking the vacuum; preferably, the gelling temperature is 65°C and the gelling time is 1h; Preparation of S2 capsule skin II: 20%-40% gelatin, 0.5-10% pectin, 8%-30% glycerin and deionized water are mixed, stirred in a gelling tank at 60-85°C for 1-3h, vacuumed and degassed until there are no bubbles, and allowed to stand for standby after breaking the vacuum; preferably, the gelling temperature is 65°C and the gelling time is 1h; Preparation of S3 core material: Use sea buckthorn oil as the base oil and add probiotic powder, homogenize in an oil tank at room temperature and set aside.

[0014] S4 pelletizing and drying to obtain finished pellets.

[0015] Furthermore, the probiotic powder is 1%-10% of the weight of the sea buckthorn oil.

[0016] Furthermore, the homogenization speed is 10-30 Hz, and the homogenization time is 10-20 min.

[0017] Furthermore, the homogenization speed was 20 Hz and the homogenization time was 15 min.

[0018] Further, step S3 also includes adding 0.1-5% of the optional material by weight to the base oil, placing it in an oil tank for homogenization at room temperature, the homogenization speed is 30-50Hz, the homogenization time is 20-40min, vacuuming and degassing during homogenization, breaking the vacuum after homogenization and stirring for standby use; adding probiotic powder, homogenizing in an oil tank at room temperature for standby use. The optional material is one or a combination of monkey mushroom powder, astragalus powder, turmeric powder, garlic oil, sweet orange oil, and mint extract, which effectively exerts the synergistic effect of the optional material components, and can shield the taste of the above-mentioned optional material components themselves, making it easy to be taken. Preferably, the homogenization speed is 40Hz and the homogenization time is 30min.

[0019] Beneficial technical effects achieved by the present invention: The present invention provides a probiotic crystal ball with a high viable bacteria rate and a preparation method thereof. The crystal ball uses seabuckthorn oil as a base oil to replace a high melting point solid fat at room temperature, which not only solves the problem that oil powder is not easy to combine, but also can be homogenized at room temperature during the preparation process, reducing the inactivation rate of probiotics; and after homogenization, the problem that the optional material particles are large and easy to block the pipe of a pill machine is solved. In addition, the application adopts a double-layer capsule skin structure, wherein the capsule skin one is a crystal ball shell, wherein the amount of glycerol is slightly more, the shell elasticity can be maintained, and the rubber skin is prevented from cracking after hardening during the drying process, thereby causing leakage, and can quickly absorb water and soften during the swallowing process, and is easy to swallow. Pectin is added to the capsule skin two and the amount of glycerol is slightly less, which can quickly harden during the drying process to form a hard shell of the crystal ball, and can tolerate gastric acid in the digestive tract at the same time, thereby maintaining the crystal ball shape without cracking, and achieving the effect of protecting the probiotics alive and reaching the intestine. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Picture of probiotic crystal spheres prepared in Example 1 of the present invention; Figure 2 Picture of the disintegration experiment of probiotic crystal spheres in Example 8 of the present invention; Figure 3 Picture of the crystal ball disintegration experiment of the commercially available product of the present invention. DETAILED DESCRIPTION

[0021] The present invention will be further described below in conjunction with specific examples. The following examples are only used to more clearly illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention.

[0022] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0023] The materials and reagents used in the embodiments of the present invention are as follows: Gelatin: Rousselot Gelatin Co., Ltd. Glycerol: Xinmeijia Biotechnology (Dalian) Co., Ltd. Pectin: Henan Yunhangxuan Biotechnology Co., Ltd. Palm oil: Heilongjiang Jiandao Biotechnology Co., Ltd. Probiotics: Jiangsu Xinshenao Biotechnology Co., Ltd. Sea buckthorn oil: Heze Zhonghe Likang Biotechnology Co., Ltd. Monkey mushroom powder, astragalus powder, etc.: Shaanxi Senfu Hi-Tech Industrial Co., Ltd. Example 1 Effect of homogenization speed on the preparation process of probiotic crystal spheres S1. Preparation of capsule skin: 24% gelatin, 16% glycerin and deionized water were mixed, stirred in a gel tank at 65°C for 1 hour, vacuumed and degassed until there were no bubbles, and allowed to stand for use after breaking the vacuum; Preparation of S2 capsule skin II: 30% gelatin, 4% pectin, 10% glycerin and deionized water were mixed, stirred in a gel tank at 65°C for 1 hour, vacuumed and degassed until there were no bubbles, and allowed to stand for use after breaking the vacuum; Preparation of S3 core material: sea buckthorn oil is used as the base oil and optional materials are added. The mixture is homogenized for the first time in an oil tank at room temperature and then used for later use. 2% probiotic powder is added to the homogenized optional materials, vacuumed and degassed, and homogenized for the second time. Optional materials include 0.5% monkey mushroom powder, 0.5% astragalus powder, and 0.5% turmeric powder.

[0024] S4 pelletizing and drying to obtain finished pellets.

[0025] The homogenization parameters of experimental groups 1-6 are shown in Table 1.

[0026] Table 1

[0027] The pictures of probiotic crystal balls obtained from experimental groups 1-6 are as follows Figure 1 As shown in AF, it can be seen from the figure that experimental groups 1-6 all obtained crystal balls with uniform structure and good roundness, and compared with the two commercially available products 1 and 2, the probiotic crystal balls prepared in this application have smaller particles and are easy to take. Parameters such as pipeline blockage and viable bacteria count were tested during the preparation process, and the test results are shown in Table 2.

[0028] The process of viable bacteria count detection is as follows: weigh 5g of probiotic crystal balls of experimental groups 1-6 and place them in a 250ml conical flask containing 45ml dissolving solution, add about 200 glass beads with a diameter of about 6mm to the flask (covering about two layers at the bottom of the conical flask), place it in a 40℃ water bath shaker, 220r / min, 20min, gradient dilution to an appropriate dilution, make 2-3 gradients, make 2 plates containing MRS solid culture medium for each gradient, anaerobic culture at 37℃ for 72±2h, and obtain the viable bacteria count by colony counting method. Among them, the dissolving solution is composed of Tween and 0.9% saline in a volume ratio of 1:3.5; the diluent is 0.9% saline.

[0029] Table 2

[0030] It can be seen from the production process records in Table 2 that when the optional materials were added to experimental groups 1-3, the first homogenization speed was 20Hz, and the first homogenization time was 20, 30, and 40min, the precipitation at the bottom of the core material tank during the transfer of the core material gradually decreased, indicating that the homogenization time can reduce the precipitation and make the suspension more uniform, but due to the insufficient intensity of the homogenization speed, the particles in the suspension were too large, resulting in the instability of the suspension and the precipitation was not eliminated. At the same time, after adding the probiotic powder, the homogenization speed was 10Hz, and the homogenization time was 10 minutes, 20 minutes, and 30 minutes, which did not significantly affect the activity of the probiotics in the product. In experimental groups 4-6, the first homogenization speed was 30, 40, and 50Hz, and the first homogenization time was 30min. The precipitation at the bottom of the core material tank disappeared during the transfer of the core material, indicating that when the homogenization speed was increased to more than 30Hz, the medicinal and edible powder was broken into smaller particles, and the stability in the suspension was strong, and no precipitation would be produced during the transfer process, thereby ensuring the uniformity of the product production. After adding probiotics, the second homogenization speed was adjusted to 20Hz, 25Hz, and 30Hz, and the activity of probiotics in the product was gradually decreasing, indicating that high homogenization speed would affect the activity of bacteria. Too high a homogenization speed would reduce bacterial activity. Therefore, choosing a suitable homogenization speed is one of the key factors in maintaining the activity of probiotics. At the same time, we can see that in experimental group 4, the pipeline was blocked after 2 hours and 23 minutes, causing the production of drop pills to stop. However, at a homogenization intensity of 30Hz, no precipitation was found in the core material tank for transferring the bacterial solution. In experimental group 6, the highest intensity homogenization speed and the longest homogenization time were used, and there was no production problem of stopping the drop pills due to blocked pipelines. However, due to the excessive homogenization intensity and obvious temperature rise, the amount of live probiotics decreased sharply. There was no problem of pipeline blockage in experimental group 5, and the decrease in the number of live bacteria was also relatively slight. The homogenization speed and homogenization time of its production were the most suitable production process conditions.

[0031] Example 2 Effect of capsule skin components on probiotic crystal spheres The probiotic crystal spheres were prepared by the method of Example 1, wherein the first homogenization speed was 40 Hz, the first homogenization time was 30 min, the second homogenization speed was 20 Hz, and the second homogenization time was 15 min. The percentage content of each component of the capsule skin 1 and the capsule skin 2 is shown in Table 3.

[0032] Table 3

[0033] The probiotic crystal balls prepared in experimental groups 7-12 were subjected to gastric acid tolerance simulation experiments to detect the viable bacterial rates of different groups. Comparative Example 1 was probiotic powder. Each group was repeated 3 times, and the results were averaged, as shown in Table 4. The experimental process is as follows: 1. Probiotic crystal ball group: prepare 3 samples, take 80 probiotic crystal balls for each sample, and place the 3 samples in artificial gastric juice respectively, and shake them at 37℃ on a shaker for 2h.

[0034] Probiotic powder group: prepare 3 samples, take 1 gram of probiotic powder for each sample, and place the 3 samples in 100 ml of artificial gastric juice respectively, and shake them at 37°C in a shaker for 2 h.

[0035] 2.① Take out the crystal balls according to the particles at 0h, 0.5h, 1h, 1.5h, and 2h to determine the number of live bacteria. Use counting method to determine the sampling amount, and take 10 particles each time. After taking out, rinse with alkaline neutralization solution before testing.

[0036] ② At regular intervals of 0h, 0.5h, 1h, 1.5h, and 2h, aspirate 1ml of the probiotic powder suspension and add it into a 9mL alkaline neutralization solution test tube, making a 10-1 gradient before subsequent testing.

[0037] 3.① Check the integrity of the crystal balls in the probiotic crystal ball group at 0h, 0.5h, 1h, 1.5h, and 2h.

[0038] Table 4

[0039] Among them, the initial number of live bacteria in the probiotic crystal spheres of the experimental groups 7-12 dissolved in artificial gastric juice was 1.0×108 CFU (100 million CFU), and the initial number of live bacteria in the control group dissolved in artificial gastric juice was 4.2 billion CFU / ml.

[0040] As shown in Table 4, after 2 hours of simulated gastric acid treatment, the viable bacteria rate of the probiotic crystal spheres of the present application is greater than 94%, while the viable bacteria rate of the comparative bacterial powder is only 35.7%. Therefore, the probiotic crystal spheres of the present application can effectively protect the probiotics from the erosion of gastric acid and maintain a high viable bacteria rate.

[0041] In addition, the disintegration experiment shows that the probiotic crystal spheres of the present application did not disintegrate after 2.5 hours in the simulated gastric acid solution. The experimental results of experimental group 8 are selected for illustration. The results are as follows: Figure 2 As shown in the figure: After 2.5 hours in the gastric acid simulated liquid, the probiotic crystal spheres of the present application remained intact, with only the capsule skin swelling. The disintegration test of the commercially available product was carried out under the same experimental environment, and the results were as follows: Figure 3 As shown. Figure 3 It can be seen that after 1.5 hours of gastric acid treatment, the capsule skin of the commercially available crystal spheres has been dissolved and signs of disintegration have appeared. After 2 hours of treatment, some of the crystal spheres have disintegrated.

[0042] The present invention has been disclosed above with preferred embodiments, but they are not intended to limit the present invention. Any technical solutions obtained by adopting equivalent replacement or equivalent transformation solutions fall within the protection scope of the present invention.

Claims

1. A probiotic crystal ball with a high viable bacteria rate, characterized in that: It includes a core material, a first bladder skin and a second bladder skin arranged in sequence from the inside to the outside; wherein: The core material includes probiotic powder and sea buckthorn oil as a carrier base oil; The weight proportions of the components of the capsule skin are as follows: 20%-40% gelatin, 8%-20% glycerol, and the rest water; The weight proportions of the components of the capsule skin II are: 20%-40% gelatin, 0.5%-10% pectin, 8%-30% glycerol, and the rest is water.

2. The probiotic crystal ball with high viable bacteria rate according to claim 1, characterized in that: The core material also includes optional materials, and the weight proportion of the optional materials is 0.1-5%.

3. The probiotic crystal ball with high viable bacteria rate according to claim 2, characterized in that: The optional materials are one or a combination of Hericium edodes powder, Astragalus powder, Turmeric powder, Garlic oil, Orange oil, and Mint extract.

4. The probiotic crystal ball with high viable bacteria rate according to claim 1, characterized in that: The weight proportions of the core material components are: 1%-10% probiotic powder, and the rest is sea buckthorn oil as a base oil; The weight proportions of the components of the capsule skin are as follows: 20%-40% gelatin, 8%-20% glycerol, and the rest water; The weight proportions of the components of the capsule skin II are: 20%-40% gelatin, 0.5%-10% pectin, 8%-30% glycerol, and the rest is water.

5. A method for preparing probiotic crystal spheres with high viable bacteria rate, characterized in that The following steps are involved: S1. Preparation of capsule skin: 20%-40% gelatin, 8%-20% glycerin and deionized water are mixed, stirred in a gel tank at 60-85°C for 1-3h, vacuumed and degassed until there are no bubbles, and allowed to stand for use after breaking the vacuum; Preparation of S2 capsule skin II: 20%-40% gelatin, 0.5-10% pectin, 8%-30% glycerin and deionized water are mixed, stirred in a gel tank at 60-85°C for 1-3h, vacuumed and degassed until there are no bubbles, and allowed to stand for standby after breaking the vacuum; Preparation of S3 core material: sea buckthorn oil is used as the base oil and probiotic powder is added, and the mixture is homogenized in an oil tank at room temperature and set aside; S4 pelletizing and drying to obtain finished pellets.

6. The method for preparing probiotic crystal spheres with high viable bacteria rate according to claim 5, characterized in that: The probiotic powder is 1%-10% of the weight of sea buckthorn oil.

7. The method for preparing probiotic crystal spheres with high viable bacteria rate according to claim 5, characterized in that: The homogenization speed is 10-30Hz, and the homogenization time is 10-20min.

8. The method for preparing probiotic crystal spheres with high viable bacteria rate according to claim 7, characterized in that: The homogenization speed was 20 Hz and the homogenization time was 15 min.

9. The method for preparing probiotic crystal spheres with high viable bacteria rate according to claim 5, characterized in that: Step S3 also includes adding 0.1-5% by weight of optional materials to the base oil, placing it in an oil tank for homogenization at room temperature, with a homogenization speed of 30-50 Hz and a homogenization time of 20-40 min, vacuuming and degassing during homogenization, breaking the vacuum after homogenization, and slowly stirring for later use; adding probiotic powder, homogenizing in an oil tank at room temperature and setting aside.

10. The method for preparing probiotic crystal spheres with high viable bacteria rate according to claim 9, characterized in that: The homogenization speed was 40 Hz and the homogenization time was 30 min.

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