Antibacterial hydrogel, preparation method and wound repair spray

Abstract

The antibacterial hydrogel comprises an antibacterial sustained-release agent and hydrogel loaded with the antibacterial sustained-release agent, and the antibacterial sustained-release agent is prepared from the following raw material components in parts by mass: 0.01 to 0.1 part of soluble metal salt, 0.5 to 1.5 parts of a cross-linking agent, 20 to 40 parts of a dispersing agent I, 1.0 to 3.5 parts of a pH regulator and 0.2 to 0.8 part of an oxidizing agent; the hydrogel loaded with the antibacterial slow-release agent comprises the following raw material components in parts by weight: 0.5-1.5 parts of gelatin, 40-50 parts of a dispersing agent II and 0.1-0.5 part of a colloid stabilizer. The antibacterial hydrogel can slowly release hydrogen peroxide to act on bacteria colonized on the wound surface, and the antibacterial effect is achieved through the strong oxidation effect of hydrogen peroxide. The invention further discloses a preparation method of the antibacterial hydrogel, and the preparation method is simple and does not need complex instruments and equipment. The invention also discloses a wound repair spray, the antibacterial hydrogel in the wound repair spray is sprayed out in a spray form and is quickly converted into a gel state, the antibacterial effect is achieved by slowly releasing hydrogen peroxide at the wound, and the skin injury is effectively repaired.

Description

Technical Field

[0001] The invention relates to the technical field of hydrogels, and in particular to an antibacterial hydrogel, a preparation method and a wound repair spray. Background Art

[0002] The skin is the largest organ in the human body and the first line of defense of the human body's natural barrier, which can protect the body from bacteria, viruses and other invasions in the external environment. Bacterial infection is the most common and unavoidable problem in skin wound healing, and it is also one of the main causes of human death worldwide. Bacterial-infected wounds are difficult to treat, and antibiotics are mainly used for treatment clinically. However, the abuse of antibiotics can lead to the emergence of multidrug-resistant bacteria. In addition, due to the lack of a suitable skin repair microenvironment, the treatment effect of antibacterial infection and wound healing is not ideal.

[0003] Hydrogel is a polymer with a three-dimensional porous network structure and excellent water retention properties. At the same time, hydrogel also has a composition and physicochemical properties similar to the extracellular matrix, and can be used as a carrier of antibacterial drugs. Skin wounds exposed to the outside are susceptible to bacterial infection. Hydrogel, as an ideal dressing, can adhere to the surface of the wound to form a protective barrier. However, since ordinary hydrogels are in a gel state at body temperature, when applied to skin wounds, they will cause secondary damage to the wound and affect wound repair.

[0004] Publication No. CN117180490A discloses a hydrogel dressing and a preparation method thereof, wherein the hydrogel dressing comprises, by weight percentage, nanoparticles loaded with glucose oxidase: 0.2wt%, gelatin modified with carboxyphenylboronic acid: 2wt%-8wt%, polyvinyl alcohol: 6wt%-10wt%, and adenine nucleoside: 1wt%-3wt%. However, the hydrogel dressing needs to be applied to the skin wound surface, which will cause secondary damage to the wound.

[0005] Publication No. CN114848894A discloses an antibacterial gel spray and a preparation method thereof, wherein the antibacterial gel spray comprises 0.5%-1% silk fibroin gel, 0.1%-0.5% potassium sorbate, 3%-10% organic acid quaternary ammonium salt, and the rest is sterile water; the preparation method comprises the following steps: diluting the silk fibroin gel, mixing the organic acid quaternary ammonium salt, adding potassium sorbate, and then forming a relatively stable complex. The antibacterial gel spray contacts the wound through the highly wettable silk fibroin, and releases the organic quaternary ammonium salt for a long time to kill bacteria, but long-term use of quaternary ammonium salt antibacterial agents will inevitably produce drug resistance, affecting the antibacterial effect. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides an antibacterial hydrogel, in which an antibacterial sustained-release agent is loaded, which can exert a good antibacterial effect and is not prone to drug resistance.

[0007] An antibacterial hydrogel, comprising an antibacterial sustained-release agent and a hydrogel loaded with the antibacterial sustained-release agent, wherein, by weight,

[0008] The raw material components of the antibacterial sustained-release agent are 0.01-0.1 parts of soluble metal salt, 0.5-1.5 parts of cross-linking agent, 20-40 parts of dispersant I, 1.0-3.5 parts of pH regulator and 0.2-0.8 parts of oxidant.

[0009] The oxidant in the present invention provides a peroxide bond and reacts with a soluble metal salt under alkaline conditions to generate an antibacterial sustained-release agent. The antibacterial sustained-release agent slowly releases hydrogen peroxide under acidic conditions. Hydrogen peroxide destroys the bacterial membrane with its strong oxidizing ability, destroys the double bonds of bacterial nucleic acids (DNA or RNA), and quickly loses its function, thereby achieving the antibacterial purpose.

[0010] The raw material components of the hydrogel loaded with the antibacterial sustained-release agent are 0.5-1.5 parts of gelatin, 40-50 parts of dispersant II, and 0.1-0.5 parts of colloid stabilizer.

[0011] The mass ratio of the antibacterial sustained-release agent to the hydrogel is 1:1-2.

[0012] The present invention uses hydrogel as a carrier of the antibacterial sustained-release agent, which can adhere to the wound surface to form a protective barrier to prevent microbial invasion and allow gas exchange, thereby providing an ideal healing environment for the wound.

[0013] During the wound healing process, infection by colonized bacteria will produce lactic acid or acetic acid, causing local acidification of the wound surface, resulting in the pH of the wound site gradually turning to an acidic level. The antibacterial sustained-release agent of the present invention can slowly release hydrogen peroxide under acidic conditions, and hydrogen peroxide kills wound colonized bacteria with strong oxidizing ability. The antibacterial sustained-release agent is loaded on a hydrogel to control its release rate. Compared with antibiotics, this antibacterial method is not easy to develop drug resistance.

[0014] Preferably, in the antibacterial sustained-release agent, the soluble metal salt is at least one of sodium salt, calcium salt, manganese salt, zinc salt, iron salt and copper salt; and the cross-linking agent is at least one of polyvinyl alcohol, polyvinyl pyrrolidone, copovidone and polysiloxane.

[0015] The cross-linking agent provided in the present invention is used as a ligand for the synthesis of the antibacterial sustained-release agent. Antibacterial sustained-release agent particles are easy to aggregate and the decomposition is not easily controlled. Therefore, the use of a cross-linking agent can prevent the antibacterial sustained-release agent from aggregating and synthesize an antibacterial sustained-release agent with uniform particle size.

[0016] Preferably, the dispersant I is at least one of ethanol, methanol, ethylene glycol and acetone.

[0017] Preferably, the pH regulator is at least one of sodium hydroxide, ammonia water and sodium carbonate.

[0018] Preferably, the oxidant is at least one of sodium peroxide, hydrogen peroxide and peracetic acid.

[0019] Further preferably, in the antibacterial sustained-release agent, the soluble metal salt is a calcium salt, and the oxidant is hydrogen peroxide.

[0020] Preferably, soluble calcium salts react with hydrogen peroxide under alkaline conditions to generate an antibacterial sustained-release agent containing calcium peroxide. Calcium peroxide, as a prodrug, stably and continuously releases calcium ions and hydrogen peroxide under acidic conditions. Calcium ions promote wound angiogenesis through calcium, PI3K / AKT and MEK1 / 2 / ERK1 / 2 signaling pathways; while hydrogen peroxide regulates inflammatory responses at the wound by killing bacteria colonizing the wound. Calcium ions and hydrogen peroxide work synergistically to promote rapid repair of the wound.

[0021] More preferably, the calcium salt is 0.05-0.1 parts, and the hydrogen peroxide is 0.5-0.8 parts.

[0022] Preferably, when the proportions of calcium salt and hydrogen peroxide are within the above range, an antibacterial sustained-release agent containing more calcium peroxide can be produced, which can release more calcium ions and hydrogen peroxide, thereby producing a better antibacterial effect.

[0023] Preferably, in the hydrogel loaded with antibacterial sustained-release agent, dispersant II is at least one of ethanol, methanol, water and propylene glycol; and the colloidal stabilizer is at least one of zinc pyrrolidone hydroxyapatite, copper pyrrolidone hydroxyapatite and sodium pyrrolidone hydroxyapatite.

[0024] The present invention also provides a method for preparing the antibacterial hydrogel, which is simple and does not require complicated instruments and equipment.

[0025] A method for preparing an antibacterial hydrogel comprises the following steps:

[0026] (1) dissolving a soluble metal salt and a cross-linking agent in a dispersant I and mixing them uniformly, adjusting the pH value with a pH adjuster, injecting an oxidant through a microinjection pump, centrifuging and washing the mixed solution, and obtaining an antibacterial sustained-release agent;

[0027] (2) dissolving gelatin and a colloidal stabilizer in a dispersant II and mixing and stirring to obtain a hydrogel;

[0028] (3) Mixing the antibacterial sustained-release agent obtained in step (1) with the hydrogel obtained in step (2) to prepare an antibacterial hydrogel.

[0029] Preferably, in step (1), the pH value is adjusted to 7-10.

[0030] Preferably, in step (1), the centrifugal speed is 7000-11000 rpm, and the centrifugal time is 10-30 min.

[0031] The present invention also provides a wound repair spray. The antibacterial hydrogel is sprayed in the form of a spray and quickly converted into a gel state, and the antibacterial effect is achieved by slowly releasing hydrogen peroxide at the wound, effectively repairing skin damage.

[0032] A wound repair spray comprises a spray device and an antibacterial hydrogel placed in the spray device. The antibacterial hydrogel is any of the above antibacterial hydrogels, and the antibacterial hydrogel is atomized and sprayed out through the spray device.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The hydrogel used in the present invention has good biocompatibility and low irritation to the skin, which can ensure safety during the treatment process. At the same time, the antibacterial sustained-release agent in the antibacterial hydrogel can slowly release hydrogen peroxide under physiological conditions. Hydrogen peroxide kills bacteria with its strong oxidizing ability and effectively repairs skin wounds. Compared with the interference of antibiotics with bacterial metabolism, it is not easy to produce drug resistance.

[0035] (2) The method for preparing the antibacterial hydrogel adopted in the present invention has simple steps, does not require complicated instruments and equipment, has low cost, and is expected to be used in industrial production.

[0036] (3) The wound repair spray prepared by the present invention is easy to use. The hydrogel is sprayed in the form of a spray and then quickly converted into a gel state, adhering to the wound. The antibacterial effect is achieved by slowly releasing hydrogen peroxide at the wound, effectively repairing skin damage, and there is no need to apply the spray to the wound, thereby avoiding the secondary damage that may be caused to the wound by the spray. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a transmission electron micrograph of the antibacterial sustained-release agent prepared in Example 1.

[0038] Figure 2 This is a plate-coated colony diagram of the antibacterial sustained-release agent prepared in Example 1 against methicillin-resistant Staphylococcus aureus.

[0039] Figure 3 This is a plate-spread colony image of the antibacterial sustained-release agent prepared in Example 1 against Escherichia coli.

[0040] Figure 41 is a comparison chart of the antibacterial ability test results of the antibacterial sustained-release agent prepared in Example 1, wherein a is a test chart of the antibacterial ability of the antibacterial sustained-release agent against methicillin-resistant Staphylococcus aureus, and b is a test chart of the antibacterial ability of the antibacterial sustained-release agent against Escherichia coli.

[0041] Figure 5 This is a morphology comparison diagram of the antibacterial hydrogel prepared in Example 1 and water.

[0042] Figure 6 This is a schematic flow chart of the wound repair spray prepared in Application Example 1.

[0043] Figure 7 These are pictures of the wound repair spray prepared in Application Example 1, wherein a is a picture of the appearance of the wound repair spray, b is a picture of the spraying process of the wound repair spray, and c is a picture of the wound repair spray after spraying.

[0044] Figure 8 This is the gelation result of the wound repair spray prepared in Application Example 1 sprayed onto a glass slide.

[0045] Fig. 9 This is a plate-coated colony image of the antibacterial effect of the wound repair spray prepared in Application Example 1 on methicillin-resistant Staphylococcus aureus.

[0046] Fig.10 This is a plate-spread colony image of the antibacterial effect of the wound repair spray prepared in Application Example 1 on Escherichia coli.

[0047] Fig.11 This is a test chart of the wound repair ability of the wound repair spray prepared in Application Example 1. DETAILED DESCRIPTION

[0048] The present invention will be further described in detail below in conjunction with the examples, but the embodiments of the present invention are not limited to the following examples.

[0049] The raw materials used in the present invention are all commercially available.

[0050] Example 1

[0051] (1) 0.05 parts of anhydrous calcium chloride and 1.5 parts of polyvinyl pyrrolidone were dissolved in 20 parts of methanol to form a mixed component, and the pH value of the mixed component was adjusted to 9 with 1.5 parts of sodium hydroxide. Then, 0.5 parts of hydrogen peroxide was injected into the mixed component through a microinjection pump, and the mixture was centrifuged at 7000 rpm for 10 minutes and washed several times to obtain an antibacterial sustained-release agent. The transmission electron microscopy image of the antibacterial sustained-release agent is shown in FIG. Figure 1 As shown;

[0052] (2) dissolving 0.8 parts of gelatin and 0.4 parts of sodium pyrrolidone hydroxyapatite in 20 parts of water, stirring and allowing to stand to obtain a hydrogel;

[0053] (3) The antibacterial sustained-release agent and the hydrogel are mixed in a ratio of 1:2 to obtain an antibacterial hydrogel.

[0054] Example 2

[0055] (1) 0.08 parts of anhydrous calcium chloride and 1.5 parts of polyvinyl pyrrolidone are dissolved in 20 parts of methanol to form a mixed component, and the pH value of the mixed component is adjusted to 9 with 1.5 parts of sodium hydroxide, and then 0.8 parts of hydrogen peroxide is injected into the mixed component through a microinjection pump, centrifuged at 7000 rpm for 10 minutes, and washed several times to obtain an antibacterial sustained-release agent;

[0056] (2) dissolving 0.8 parts of gelatin and 0.4 parts of sodium pyrrolidone hydroxyapatite in 20 parts of water, stirring and allowing to stand to obtain a hydrogel;

[0057] (3) The antibacterial sustained-release agent and the hydrogel are mixed in a ratio of 1:2 to obtain an antibacterial hydrogel.

[0058] Example 3

[0059] (1) dissolving 0.05 parts of anhydrous sodium chloride and 1.5 parts of polyvinyl alcohol in 20 parts of methanol to form a mixed component, adjusting the pH value of the mixed component to 9 with 1.5 parts of sodium hydroxide, and then injecting 0.5 parts of sodium peroxide into the mixed component through a microinjection pump, centrifuging at 7000 rpm for 10 minutes, and washing several times to obtain an antibacterial sustained-release agent;

[0060] (2) dissolving 0.8 parts of gelatin and 0.4 parts of sodium pyrrolidone hydroxyapatite in 20 parts of water, stirring and allowing to stand to obtain a hydrogel;

[0061] (3) The antibacterial sustained-release agent and the hydrogel are mixed in a ratio of 1:2 to obtain an antibacterial hydrogel.

[0062] Example 4

[0063] (1) 0.1 parts of anhydrous zinc chloride and 1.5 parts of polyvinyl alcohol are dissolved in 20 parts of methanol to form a mixed component, 1.5 parts of sodium hydroxide are used to adjust the pH value of the mixed component to 9, and then 0.5 parts of sodium peroxide is injected into the mixed component through a microinjection pump, centrifuged at 7000 rpm for 10 minutes, and washed several times to obtain an antibacterial sustained-release agent;

[0064] (2) dissolving 0.8 parts of gelatin and 0.4 parts of sodium pyrrolidone hydroxyapatite in 20 parts of water, stirring and allowing to stand to obtain a hydrogel;

[0065] (3) The antibacterial sustained-release agent and the hydrogel are mixed in a ratio of 1:2 to obtain an antibacterial hydrogel.

[0066] Example 5

[0067] (1) dissolving 0.05 parts of anhydrous zinc chloride and 1.5 parts of polyvinyl alcohol in 20 parts of methanol to form a mixed component, adjusting the pH value of the mixed component to 9 with 1.5 parts of sodium hydroxide, and then injecting 0.5 parts of sodium peroxide into the mixed component through a microinjection pump, centrifuging at 7000 rpm for 10 minutes, and washing several times to obtain an antibacterial sustained-release agent;

[0068] (2) dissolving 0.8 parts of gelatin and 0.4 parts of copper pyrrolidone hydroxyapatite in 25 parts of water, stirring and allowing to stand to obtain a hydrogel;

[0069] (3) The antibacterial sustained-release agent and the hydrogel are mixed in a ratio of 1:2 to obtain an antibacterial hydrogel.

[0070] Performance test: The performance test of the antibacterial hydrogel prepared in Example 1 was carried out, specifically:

[0071] (1) Using transmission electron microscopy to characterize the morphology of the antibacterial sustained-release agent prepared in Example 1

[0072] Result analysis: Figure 1 As shown, the prepared antibacterial sustained-release agent has a uniform morphology and good dispersibility and uniformity.

[0073] (2) Antibacterial ability test of the antibacterial sustained-release agent synthesized in Example 1

[0074] A. Take out the frozen methicillin-resistant Staphylococcus aureus (MRSA) and incubate it with LB liquid medium for 24 hours. Incubate the activated bacteria with PBS and the antibacterial sustained-release agent synthesized in Example 1 at a volume ratio of 2:1, and perform plate coating after 6 hours. Use an ELISA reader to measure the absorbance signal intensity at 600nm.

[0075] The test results of methicillin-resistant Staphylococcus aureus are as follows: Figure 2 and Figure 4 As shown in a, wherein: after being treated with the antibacterial sustained-release agent, the number of MRSA colonies was greatly reduced, proving that the antibacterial sustained-release agent has antibacterial activity against MRSA, and the antibacterial rate against MRSA reaches 87%.

[0076] B. Take out the frozen Escherichia coli (E. coli) and incubate with LB liquid medium for 24 hours. Incubate the activated bacteria with PBS and the antibacterial sustained-release agent synthesized in Example 1 at a volume ratio of 2:1, and perform plate coating after 6 hours. Use an ELISA reader to measure the absorbance signal intensity at 600nm.

[0077] The test results of Escherichia coli are as follows Figure 3 and Figure 4 As shown in b, after being treated with the antibacterial sustained-release agent, the number of E. coli colonies was greatly reduced, proving that the antibacterial sustained-release agent also has antibacterial activity against E. coli, and the inhibition rate against E. coli reaches 92%.

[0078] (3) Characterization of the state of the antibacterial hydrogel prepared in Example 1

[0079] Figure 5 The oblique state of water and the antibacterial hydrogel prepared by the present invention is shown. After the antibacterial hydrogel is cooled and inverted, it does not flow, indicating that the transformation of the antibacterial sustained-release agent from solution to gel is completed.

[0080] Application Example 1: The antibacterial hydrogel prepared in Example 1 is placed in a spray device to prepare a wound repair spray, such as Figure 6 As shown in the schematic diagram.

[0081] Performance test: The wound repair spray prepared according to Example 1 was subjected to performance test, specifically:

[0082] (1) Characterization of the state of the wound repair spray prepared in Example 1

[0083] The prepared wound repair spray is in the upright state ( Figure 7 a) Spraying process ( Figure 7 b) The sprayed gel state ( Figure 7 c and Figure 8 ). The antibacterial hydrogel is converted into a solution by the pressure of the spray bottle and sprayed out. The solution instantly turns into a gel state again and has very low fluidity.

[0084] (2) Antibacterial test results of the wound repair spray prepared according to Example 1

[0085] A. Take out the frozen methicillin-resistant Staphylococcus aureus (MRSA) and incubate it with LB liquid medium for 24 hours. Use a UV spectrophotometer to measure OD 600 =1, diluted to 10 with sterile PBS 6 CFU / mL. MRSA diluted with sterile PBS was evenly spread on LB agar medium, and two groups were spread in total. They were treated randomly, and one group was not treated as a blank control; the other group was treated with wound repair spray (about 100uL), shaken evenly, and fully dispersed in the bacteria-treated LB agar medium, incubated at 37℃ overnight, and photographed for observation.

[0086] The test results of methicillin-resistant Staphylococcus aureus are as follows: Fig. 9As shown, among them: after being treated with the wound repair spray, the number of MRSA colonies was greatly reduced, proving that the antibacterial sustained-release agent still has antibacterial activity against MRSA after being prepared into the wound repair spray.

[0087] B. Take out the frozen E. coli and incubate it with LB liquid medium for 24 hours. Use a UV spectrophotometer to measure OD 600 =1, diluted to 10 with sterile PBS 6 CFU / mL. E. coli diluted with sterile PBS was evenly spread on LB agar medium, and two groups were spread in total. They were treated randomly, and one group was not treated as a blank control; the other group was treated with wound repair spray (about 100uL), shaken evenly, and fully dispersed in the bacteria-treated LB agar medium, incubated at 37℃ overnight, and photographed for observation.

[0088] The test results of Escherichia coli are as follows Fig.10 As shown, after being treated with the wound repair spray, the number of E. coli colonies was greatly reduced, proving that the antibacterial sustained-release agent still has antibacterial activity against E. coli after being prepared into the wound repair spray.

[0089] (3) Evaluation of the wound repair ability of the wound repair spray prepared according to Example 1

[0090] Prepare 6-7 week old BALB / c male mice and keep them in an SPF room for one week. Methicillin-resistant Staphylococcus aureus (MRSA) was purified in advance and cultured overnight in LB medium. OD was measured using a UV spectrophotometer. 600 =1, diluted to 10 with sterile PBS 8 CFU / mL. On day -1, the mice were anesthetized, and the backs of the mice were depilated with a razor and depilatory cream, and 75% alcohol was sprayed for disinfection. A sterile scalpel was used to make wounds of basically the same area, and then 50uL of diluted MRSA bacterial solution was added drop by drop to the wound to make an infectious wound model. The mice were randomly divided into a blank control group and a treatment group. The treatment group used a wound repair spray to treat the wound on days 0 and 3 (about 100uL), and photos were taken on days 0, 1, 3, 5, and 7 to observe the healing of the wound.

[0091] Fig.11 This is a test chart of the wound repair ability of the wound repair spray prepared in Application Example 1. As shown in the figure, after using the wound repair spray, the wound repair ability of the treatment group is significantly better than that of the blank control group.

[0092] The embodiments described above provide a detailed description of the technical solutions of the present invention. It should be understood that the above are only specific examples of the present invention and are not intended to limit the present invention. Any modifications, supplements or similar substitutions made within the scope of the principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibacterial hydrogel, characterized in that: Including antibacterial sustained-release agent and hydrogel loaded with antibacterial sustained-release agent, by weight, The raw material components of the antibacterial sustained-release agent are 0.01-0.1 parts of soluble metal salt, 0.5-1.5 parts of cross-linking agent, 20-40 parts of dispersant I, 1.0-3.5 parts of pH regulator, and 0.2-0.8 parts of oxidant; The raw material components of the hydrogel loaded with the antibacterial sustained-release agent are 0.5-1.5 parts of gelatin, 40-50 parts of dispersant II, and 0.1-0.5 parts of colloid stabilizer.

2. The antibacterial hydrogel according to claim 1, characterized in that: The mass ratio of the antibacterial sustained-release agent to the hydrogel is 1:1-2.

3. The antibacterial hydrogel according to claim 1, characterized in that: In the antibacterial sustained-release agent, the soluble metal salt is at least one of sodium salt, calcium salt, manganese salt, zinc salt, iron salt and copper salt; The cross-linking agent is at least one of polyvinyl alcohol, polyvinyl pyrrolidone, copolyvidone and polysiloxane; The dispersant I is at least one of ethanol, methanol, ethylene glycol and acetone; The pH regulator is at least one of sodium hydroxide, ammonia water and sodium carbonate; The oxidant is at least one of sodium peroxide, hydrogen peroxide and peracetic acid.

4. The antibacterial hydrogel according to claim 3, characterized in that: In the antibacterial sustained-release agent, the soluble metal salt is a calcium salt and the oxidant is hydrogen peroxide.

5. The antibacterial hydrogel according to claim 4, characterized in that: The calcium salt is 0.05-0.1 part, and the hydrogen peroxide is 0.5-0.8 part.

6. The antibacterial hydrogel according to claim 1, characterized in that: In the hydrogel, the dispersant II is at least one of ethanol, methanol, water, and propylene glycol; The colloidal stabilizer is at least one of zinc pyrrolidone hydroxyapatite, copper pyrrolidone hydroxyapatite and sodium pyrrolidone hydroxyapatite.

7. The method for preparing the antibacterial hydrogel according to any one of claims 1 to 6, comprising the following steps: (1) dissolving a soluble metal salt and a cross-linking agent in a dispersant I and mixing them uniformly, adjusting the pH value with a pH adjuster, injecting an oxidant through a microinjection pump, centrifuging and washing the mixed solution, and obtaining an antibacterial sustained-release agent; (2) dissolving gelatin and a colloidal stabilizer in a dispersant II and mixing and stirring to obtain a hydrogel; (3) Mixing the antibacterial sustained-release agent obtained in step (1) with the hydrogel obtained in step (2) to prepare an antibacterial hydrogel.

8. The method for preparing the antibacterial hydrogel according to claim 7, characterized in that: In step (1), the pH value is adjusted to 7-10.

9. The method for preparing the antibacterial hydrogel according to claim 7, characterized in that: In step (1), the centrifugal speed is 7000-11000 rpm, and the centrifugal time is 10-30 min.

10. A wound repair spray, comprising a spray device and an antibacterial hydrogel placed in the spray device, characterized in that: The antibacterial hydrogel comprises the antibacterial hydrogel according to any one of claims 1 to 6, and the antibacterial hydrogel is atomized and sprayed out through a spray device.

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