Preparation method of antibacterial PEEK material for bone implantation

Abstract

The invention discloses a preparation method of an antibacterial PEEK material for bone implantation. The preparation method comprises the following steps: carrying out sand blasting treatment on the surface of a PEEK sample; immersing the sandblasted PEEK sample into a mixed solution containing concentrated sulfuric acid and hydrogen peroxide, and carrying out chemical sulfonation treatment; immersing the sulfonated PEEK sample into a dopamine solution, so that dopamine is oxidized and self-polymerized on the surface to form a PDA coating; immersing the PEEK sample with the PDA coating into an antibacterial drug solution, so that the antibacterial drug is loaded on the surface; carrying out heat treatment on the PEEK sample loaded with the antibacterial drug; thoroughly cleaning the sample, removing uncombined antibacterial drug molecules, and drying; through sand blasting treatment and sulfonation treatment, the surface roughness and hydrophilicity of the PEEK material are remarkably improved, and favorable conditions are provided for adhesion of a dopamine coating and an antibacterial agent; due to introduction of the dopamine coating, the biocompatibility and the adhesion of the material are enhanced, and the dopamine coating also serves as an effective carrier of antibacterial drugs, so that the antibacterial function is maximized.

Description

Technical Field

[0001] The invention relates to the technical field of bone implant materials, and in particular to a method for preparing an antibacterial PEEK material for bone implantation. Background Art

[0002] With the continuous advancement of medical technology, bone implant materials play an increasingly important role in orthopedic surgery and restorative treatment. Although traditional bone implant materials such as metals, ceramics and polymers have met clinical needs to a certain extent, there are still some problems that need to be solved. Among them, the most prominent is the long-term stability and biocompatibility of implant materials in the body. Although metal and ceramic materials have high mechanical strength, their biological inertness leads to weak bonding ability with surrounding bone tissue, which can easily cause loosening and rejection of the implant. Traditional polymer materials such as polyethylene and polypropylene, although they have good biocompatibility, have poor antibacterial properties and can easily lead to postoperative infection, affecting the patient's recovery effect.

[0003] In addition, with the increasing problem of population aging, the incidence of orthopedic diseases has increased year by year, and the demand for bone implant materials has also increased. Therefore, the development of a new bone implant material that has good biocompatibility and can effectively inhibit bacterial infection has become a research hotspot in the current medical and material science fields.

[0004] In this context, polyetheretherketone (PEEK), as a high-performance polymer material, has gradually attracted the attention of researchers due to its excellent mechanical properties, good biocompatibility and processability. However, the original PEEK material still has deficiencies in antibacterial properties and is difficult to meet the high requirements for antibacterial properties in clinical applications.

[0005] Therefore, it is necessary to provide a method for preparing an antibacterial PEEK material for bone implantation to solve the above technical problems. Summary of the invention

[0006] The invention overcomes the shortcomings of the prior art and provides a method for preparing an antibacterial PEEK material for bone implantation.

[0007] To achieve the above object, the technical solution adopted by the present invention is: a method for preparing an antibacterial PEEK material for bone implantation, comprising the following steps:

[0008] S1: sandblasting the surface of the PEEK sample using abrasive particles to change its surface roughness;

[0009] S2: The sandblasted PEEK sample is immersed in a mixed solution of concentrated sulfuric acid and hydrogen peroxide for chemical sulfonation treatment;

[0010] S3: immersing the sulfonated PEEK sample in a dopamine solution to allow dopamine to oxidize and self-polymerize on the surface to form a polydopamine (PDA) coating;

[0011] S4: immersing the PEEK sample with PDA coating into an antimicrobial drug solution to load the antimicrobial drug on the surface;

[0012] S5: mild heat treatment of PEEK samples after antimicrobial loading;

[0013] S6: Wash the sample thoroughly to remove unbound antimicrobial drug molecules and dry it.

[0014] In a preferred embodiment of the present invention, in S1, the sandblasting uses silica abrasive particles with an average particle size of 50-100 μm, the working pressure is set to 0.4-0.6 MPa, the spraying angle is perpendicular to the PEEK surface, the distance between the nozzle and the surface is about 10-15 cm, and the sandblasting duration is 30-60 seconds until the target roughness Ra reaches about 1-2 μm.

[0015] In a preferred embodiment of the present invention, in S2, the volume ratio of the mixed solution of concentrated sulfuric acid and hydrogen peroxide is 2:1-4:1, and the reaction is carried out at room temperature for 1 hour.

[0016] In a preferred embodiment of the present invention, in S3, the concentration of dopamine solution is 2 mg / mL, Tris-HCl buffer with pH 8.5 is used as solvent, and the PDA coating deposition time is 20-30 hours, during which the solution is kept static.

[0017] In a preferred embodiment of the present invention, in S4, the antimicrobial drug is antimicrobial peptide KR-12, the purity of which is not less than 95%, and the concentration of the antimicrobial drug solution is adjusted according to the specific drug, such as 1 mg / mL, and the soaking time is 4 hours.

[0018] In a preferred embodiment of the present invention, in said S5, the heat treatment condition is incubating at 35-40°C for 1-3h.

[0019] In a preferred embodiment of the present invention, in S1, after the sandblasting is completed, the PEEK surface is rinsed with deionized water until no abrasive residue is visible to the naked eye, then rinsed with an ethanol solution to remove surface stains, and dried at 55-65° C. for 1-3 hours.

[0020] In a preferred embodiment of the present invention, in S2, after the sulfonation treatment, a large amount of deionized water is used to rinse until it becomes neutral, and the mixture is dried naturally.

[0021] In a preferred embodiment of the present invention, in S3, after the deposition is completed, the sample surface is rinsed with pure water for several times to remove unbound dopamine molecules, and then dried naturally at room temperature or placed in a vacuum drying oven for 3-5 hours.

[0022] In a preferred embodiment of the present invention, in S6, the sample is washed with physiological saline and the drying temperature is set at 20-60°C.

[0023] In a preferred embodiment of the present invention,.

[0024] The present invention solves the defects existing in the background technology and has the following beneficial effects:

[0025] (1) The present invention provides a method for preparing an antibacterial PEEK material for bone implantation. In the process of preparing the antibacterial PEEK material, the combination of sandblasting and sulfonation plays a vital synergistic role; sandblasting effectively changes the roughness of the surface of the PEEK sample through the impact of silica abrasive particles, increases the surface area, and provides more attachment points for subsequent chemical modification; while sulfonation uses a mixed solution of concentrated sulfuric acid and hydrogen peroxide to introduce sulfonic acid groups on the PEEK surface, which not only enhances the hydrophilicity of the material, but also provides more chemical binding sites for the attachment of dopamine. This dual treatment makes the PEEK surface both physically rough and chemically active, thereby significantly improving the stability and firmness of the dopamine coating and subsequent antibacterial drug loading.

[0026] (2) In the present invention, the PDA coating not only has good biocompatibility and adhesion, but also can serve as an effective carrier of antimicrobial drugs. When the sulfonated PEEK sample is immersed in a dopamine solution, the dopamine molecules are rapidly oxidized and self-polymerized on the surface to form a uniform and dense PDA coating. When the PEEK sample with the PDA coating is immersed in an antimicrobial peptide KR-12 solution, the antimicrobial peptide molecules are firmly bound to the PDA coating through electrostatic effects, hydrogen bonds or covalent bonds. This binding method not only increases the loading amount of the antimicrobial drug, but also ensures its uniform distribution on the surface of the material and its long-term stability. At the same time, the presence of the PDA coating also enhances the sustained release effect of the antimicrobial peptide, enabling it to continuously and effectively exert its antimicrobial effect.

[0027] (3) In the present invention, the surface roughness and hydrophilicity of the PEEK material are significantly improved through sandblasting and sulfonation treatment, providing favorable conditions for the adhesion of the dopamine coating and antibacterial drugs; on the other hand, the introduction of the dopamine coating not only enhances the biocompatibility and adhesion of the material, but also serves as an effective carrier of antibacterial drugs to maximize the antibacterial function; in addition, the mild heat treatment and thorough cleaning steps further ensure the purity and stability of the material. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.

[0029] Figure 1 It is a flow chart of a preferred embodiment of the present invention. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0032] like Figure 1 As shown, the present invention provides a method for preparing an antibacterial PEEK material for bone implantation, comprising the following steps:

[0033] S1: The surface of the PEEK sample is sandblasted. The sandblasting uses silica abrasive particles with an average particle size of 50-100μm. The working pressure is set to 0.4-0.6MPa. The spray angle is perpendicular to the PEEK surface. The distance between the nozzle and the surface is about 10-15cm. The sandblasting duration is 30-60 seconds until the target roughness Ra is about 1-2μm. Abrasive particles are used to change its surface roughness. After the sandblasting, the PEEK surface is rinsed with deionized water until there is no visible abrasive residue, then rinsed with ethanol solution to remove surface stains, and dried at 55-65℃ for 1-3h.

[0034] It should be noted that: S1 ensures the uniformity of roughness by adjusting the surface roughness to the range of Ra1-2μm, and builds a solid foundation for coating deposition in S3 and drug loading in S4. This not only significantly improves the cleanliness of the PEEK surface, effectively removes dirt, grease and tiny impurities, and makes the surface reach a highly clean state, but also strengthens the mechanical locking effect between the surface and subsequent coatings by increasing the roughness, thereby greatly improving the adhesion and durability of the coating; the drying treatment removes residual moisture on the surface to avoid coating curing defects or cracks that may be caused by residual moisture, creating ideal substrate conditions for subsequent sulfonation treatment and PDA coating deposition.

[0035] S2: The sandblasted PEEK sample is immersed in a mixed solution of concentrated sulfuric acid and hydrogen peroxide, the volume ratio of the mixed solution of concentrated sulfuric acid and hydrogen peroxide is 2:1-4:1, and reacted at room temperature for 1 hour for chemical sulfonation treatment. After sulfonation treatment, it is rinsed with a large amount of deionized water until neutral and dried naturally.

[0036] It should be noted that: S2 performs chemical sulfonation treatment on the surface of the PEEK sample, using a mixed solution of concentrated sulfuric acid and hydrogen peroxide, and the sulfonation reaction can be effectively carried out at room temperature, avoiding material deformation or degradation problems that may be caused by high-temperature treatment, while simplifying the operation process, and can introduce sulfonic acid groups on the PEEK surface, which not only improves the hydrophilicity of the surface, but also significantly enhances its chemical reactivity; the introduction of sulfonic acid groups provides abundant binding sites for the deposition of the dopamine coating in S3, greatly enhancing the bonding force between the coating and the substrate, so that the dopamine coating can be firmly attached to the PEEK surface, thereby improving the stability and durability of the coating.

[0037] S3: The sulfonated PEEK sample is immersed in a dopamine solution with a concentration of 2 mg / mL. Tris-HCl buffer with a pH of 8.5 is used as the solvent. The PDA coating deposition time is 20-30 hours. During this period, the solution is kept static to allow dopamine to oxidize and self-polymerize on the surface to form a polydopamine (PDA) coating. After deposition, the sample surface is rinsed with pure water several times to remove unbound dopamine molecules, and then dried naturally at room temperature or placed in a vacuum drying oven for 3-5 hours.

[0038] It should be noted that: S3 forms a PDA coating on the surface of the PEEK sample through the spontaneous oxidation and self-polymerization reaction of dopamine under alkaline conditions. This treatment process not only utilizes the good adhesion and biocompatibility of dopamine, but also ensures the full polymerization of dopamine molecules on the surface through a deposition time of 20-30 hours; the formation of the PDA coating enhances the biological activity and functionality of the PEEK surface, opening up more possibilities for the subsequent drug loading of S4, including but not limited to the stable loading and slow release of antibacterial drugs; the PDA coating not only enhances the interaction between the drug and the substrate.

[0039] S4: Immerse the PEEK sample with PDA coating in an antimicrobial drug solution. The antimicrobial drug is antimicrobial peptide KR-12 with a purity of not less than 95%. The concentration of the antimicrobial drug solution is adjusted according to the specific drug, such as 1 mg / mL. The immersion time is 4 hours, so that the antimicrobial drug is loaded on the surface.

[0040] It should be noted that: Based on the PDA coating, S4 achieves stable loading of antimicrobial drugs through the immersion method, giving the PEEK material strong antimicrobial properties, which lies in its flexibility and diversity, and can select a variety of antimicrobial drugs for loading to meet the specific needs of different application scenarios. In effect, the antimicrobial drugs are successfully loaded on the surface of the PDA coating, which not only effectively inhibits the growth and reproduction of microorganisms, but also achieves slow release of drugs through the regulation of the PDA coating, thereby prolonging the antimicrobial effect of the material.

[0041] S5: The PEEK sample loaded with antimicrobial drugs was incubated at 35-40°C for 1-3 h.

[0042] It should be noted that S5 implements mild heat treatment to help the antimicrobial drugs more firmly bind to the coating, improve the durability of the drug load, and ensure a lower processing temperature, effectively avoiding drug inactivation or material deformation problems that may be caused by high temperature; and enhance the bonding force between the antimicrobial drugs and the PDA coating, significantly reducing the risk of drug shedding during subsequent use.

[0043] S6: Wash the sample with saline to remove unbound antimicrobial drug molecules, and dry it at a temperature ranging from 20 to 60°C.

[0044] It should be noted that S6 can effectively remove unbound antimicrobial drug molecules and possible residues by washing with saline.

[0045] In summary: through the steps of sandblasting, sulfonation, PDA coating deposition and antimicrobial drug loading, the surface of PEEK material is successfully loaded with antimicrobial drugs, which significantly improves its antibacterial properties and effectively inhibits the growth of microorganisms; and the sandblasting treatment increases the surface roughness, the sulfonation treatment introduces sulfonic acid groups, and the PDA coating provides good adhesion. These treatments together enhance the bonding strength between the coating and the substrate, making the coating more stable and durable.

[0046] Embodiment 1:

[0047] S1: A piece of PEEK sample was selected as the treatment object, and sandblasting was performed using silica abrasive particles with an average particle size of 75 μm. The working pressure was set to 0.5 MPa, the spray angle was kept perpendicular to the PEEK surface, the distance between the nozzle and the surface was about 12.5 cm, and the sandblasting lasted for 45 seconds until the target roughness Ra reached about 1.5 μm. After the treatment, the PEEK surface was immediately rinsed with deionized water until no abrasive residue was visible to the naked eye, and then the surface was rinsed with 95% ethanol solution to remove residual stains, and dried at 60°C for 2 hours.

[0048] S2: The sandblasted PEEK sample was immersed in a mixed solution of concentrated sulfuric acid and hydrogen peroxide with a volume ratio of 3:1. The PEEK sample was completely immersed and reacted at room temperature for 1 hour. During the reaction, it was gently stirred to ensure uniform sulfonation. After the reaction, the PEEK sample was rinsed with a large amount of deionized water until the rinse solution was neutral, and then dried naturally;

[0049] S3: Prepare a dopamine solution with a concentration of 2 mg / mL, use Tris-HCl buffer with pH 8.5 as the solvent, immerse the sulfonated PEEK sample in the dopamine solution, ensure that the model is completely immersed, and let it stand at room temperature for 24 hours to allow dopamine to oxidize and self-polymerize on the surface to form a PDA coating. After the deposition is completed, rinse the sample surface with pure water several times to remove unbound dopamine molecules, and then dry it naturally at room temperature for 4 hours to ensure that the coating is completely cured;

[0050] S4: Prepare an antimicrobial peptide KR-12 solution with a concentration of 1 mg / mL, ensure that the purity of the solution is not less than 95%, immerse the PEEK sample with PDA coating in the antimicrobial peptide KR-12 solution for 4 hours, and after the immersion, gently take out the sample to avoid damaging the surface coating;

[0051] S5: The PEEK sample loaded with antimicrobial drugs was placed in a 37.5°C incubator for 2 h;

[0052] S6: Wash the sample thoroughly with saline to remove unbound antimicrobial drug molecules and residues. After washing, dry the sample at 40°C for 2 hours to ensure that the sample surface is dry and free of moisture.

[0053] Embodiment 2:

[0054] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 0.2 mg / mL.

[0055] Embodiment three:

[0056] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 0.4 mg / mL.

[0057] Embodiment 4:

[0058] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 0.6 mg / mL.

[0059] Embodiment five:

[0060] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 0.8 mg / mL.

[0061] Embodiment six:

[0062] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 1.2 mg / mL.

[0063] Embodiment seven:

[0064] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 1.4 mg / mL.

[0065] Embodiment eight:

[0066] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 1.6 mg / mL.

[0067] Embodiment nine:

[0068] This embodiment is basically the same as Embodiment 1, except that in step S4, the concentration of the antimicrobial peptide KR-12 solution is changed to 1.8 mg / mL.

[0069] Embodiment ten:

[0070] This embodiment is basically the same as Embodiment 1, except that step S1 is removed.

[0071] Embodiment eleven:

[0072] This embodiment is basically the same as Embodiment 1, except that step S2 is removed.

[0073] Embodiment 12:

[0074] This embodiment is basically the same as Embodiment 1, except that step S3 is removed.

[0075] Experiment 1:

[0076] Prepare samples: Prepare PEEK samples according to the methods of Examples 1 to 9, respectively. For each example, prepare enough samples for various bacteria tests and hydrophilicity tests.

[0077] Preparation of bacterial suspension: Prepare bacterial suspensions of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa separately, ensuring that the initial concentration of each bacterium is the same at 10^6 CFU / mL.

[0078] Antibacterial test: Each bacterial suspension was inoculated into a sterile culture dish or test tube containing the corresponding PEEK sample; incubated at an appropriate temperature (such as 37°C) for a certain time (such as 24 hours) to ensure sufficient bacterial growth. After the incubation, an appropriate amount of incubation solution was taken for colony counting and the antibacterial rate was calculated.

[0079] Calculation of antibacterial rate:

[0080] Inhibition rate = (number of colonies in the control group - number of colonies in the experimental group) / number of colonies in the control group × 100%.

[0081] 2. Hydrophilicity test

[0082] Equipment preparation: A contact angle meter, which can accurately measure the contact angle formed when a liquid drops on a solid surface.

[0083] Test steps: fix the PEEK sample of each embodiment on the sample stage of the contact angle meter; use a micro syringe to drop a drop of deionized water on the sample surface; immediately use the contact angle meter to capture the image of the moment when the drop contacts the sample surface, and automatically measure the contact angle; record the contact angle value of each sample.

[0084] Table 1:

[0085]

[0086]

[0087] It can be seen from Table 1 that as the concentration of antimicrobial peptide KR-12 increases, the inhibition rate of PEEK samples against Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus gradually increases; from Example 2 (0.2 mg / mL) to Example 6 (1.2 mg / mL), the inhibition rate increases significantly, indicating that the increase in the concentration of antimicrobial peptides can more effectively inhibit bacterial growth; however, when the concentration of antimicrobial peptides continues to increase to Example 7 (1.4 mg / mL) and above, the increase in the inhibition rate tends to be flat, indicating that after reaching a certain concentration, increasing the concentration of antimicrobial peptides has limited effect on further improving the antibacterial effect.

[0088] The antibacterial rate of Example 10 (sandblasting treatment removed) is significantly lower than that of other examples, indicating that sandblasting plays an important role in improving the antibacterial properties of the material, and may promote the attachment and release of antimicrobial peptides by increasing the surface roughness.

[0089] The antibacterial rate of Example 11 (removal of sulfonation treatment) decreased slightly, but was still higher than that of Example 10, indicating that although sulfonation treatment contributes to improving the antibacterial performance, its effect may not be as significant as that of sandblasting treatment.

[0090] The antibacterial rate of Example 12 (removal of dopamine coating) dropped significantly, indicating that the dopamine coating plays an important role in the attachment of antimicrobial peptides and the exertion of antibacterial effects.

[0091] Effect of dopamine coating on hydrophilicity: The samples treated with sulfonation and dopamine coating (Examples 1 to 9) all showed high hydrophilicity (smaller contact angles) because the sulfonation treatment introduced hydrophilic groups, while the dopamine coating further enhanced the hydrophilicity of the surface.

[0092] The hydrophilicity of the samples after the sulfonation or dopamine coating removal (Examples 10, 11, and 12) was significantly reduced, especially Example 12 (dopamine coating removal) had the largest contact angle, indicating that the dopamine coating plays an important role in improving the hydrophilicity of the material.

[0093] In summary:

[0094] The concentration of antimicrobial peptide KR-12 has a significant effect on the antibacterial properties of PEEK materials. Appropriately increasing the concentration can increase the inhibition rate, but too high a concentration will not significantly improve the effect. Sandblasting, sulfonation and dopamine coating are key steps to improve the antibacterial properties of PEEK materials, and none of them can be missing. Sulfonation and dopamine coating treatments significantly improve the hydrophilicity of PEEK materials, which helps the adhesion of antimicrobial peptides and the improvement of antibacterial effects.

[0095] The above is based on the ideal embodiment of the present invention. Through the above description, relevant personnel can make various changes and modifications without departing from the technical concept of the present invention. The technical scope of the present invention is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A method for preparing an antibacterial PEEK material for bone implantation, characterized in that: The following steps are involved: S1: sandblasting the surface of the PEEK sample using abrasive particles to change its surface roughness; S2: The sandblasted PEEK sample is immersed in a mixed solution of concentrated sulfuric acid and hydrogen peroxide for chemical sulfonation treatment; S3: immersing the sulfonated PEEK sample in a dopamine solution to allow dopamine to oxidize and self-polymerize on the surface to form a polydopamine (PDA) coating; S4: immersing the PEEK sample with PDA coating into an antimicrobial drug solution to load the antimicrobial drug on the surface; S5: mild heat treatment of PEEK samples after antimicrobial loading; S6: Wash the sample thoroughly to remove unbound antimicrobial drug molecules and dry it.

2. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In S1, the sandblasting uses silica abrasive particles with an average particle size of 50-100 μm, the working pressure is set to 0.4-0.6 MPa, the spray angle is perpendicular to the PEEK surface, the distance between the nozzle and the surface is about 10-15 cm, and the sandblasting duration is 30-60 seconds until the target roughness Ra reaches about 1-2 μm.

3. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In the S2, the volume ratio of the mixed solution of concentrated sulfuric acid and hydrogen peroxide is 2:1-4:1, and the reaction is carried out at room temperature for 1 hour.

4. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In the S3, the concentration of the dopamine solution is 2 mg / mL, a Tris-HCl buffer with a pH of 8.5 is used as a solvent, and the PDA coating deposition time is 20-30 hours, during which the solution is kept static.

5. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In S4, the antimicrobial drug is antimicrobial peptide KR-12, the purity of which is not less than 95%, the concentration of the antimicrobial drug solution is adjusted according to the specific drug, such as 1 mg / mL, and the soaking time is 4 hours.

6. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In the S5, the heat treatment condition is incubating at 35-40°C for 1-3h.

7. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In S1, after the sandblasting treatment is completed, the PEEK surface is rinsed with deionized water until no abrasive residue is visible to the naked eye, and then rinsed with an ethanol solution to remove surface stains, and dried at 55-65° C. for 1-3 hours.

8. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In the S2, after the sulfonation treatment, a large amount of deionized water is used to rinse to neutrality, and the mixture is naturally dried.

9. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In S3, after the deposition is completed, the sample surface is rinsed with pure water for several times to remove unbound dopamine molecules, and then dried naturally at room temperature or placed in a vacuum drying oven for 3-5 hours.

10. The method for preparing an antibacterial PEEK material for bone implantation according to claim 1, characterized in that: In S6, the sample is washed with physiological saline, and the drying temperature is set at 20-60°C.

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