Method for increasing dissolution rate of beta-1, 3 / alpha-1, 3-glucan

Abstract

The invention provides a method for increasing the dissolution rate of beta-1, 3 / alpha-1, 3-glucan, which comprises the following steps: a, taking beta-1, 3 / alpha-1, 3-glucan as a raw material, spraying an adhesive into the beta-1, 3 / alpha-1, 3-glucan, uniformly mixing, wetting, and preparing a soft material; b, sieving the soft material prepared in the step a through a 30-40-mesh sieve, and granulating; and c, drying to obtain the beta-1, 3 / alpha-1, 3-glucan particles. According to the method disclosed by the invention, the optimal particle size range is screened and determined by accurately controlling the preparation process of the glucan particles, so that the rapid dissolution of the glucan particle preparation is realized. The method not only maintains the biological activity and purity of the glucan, but also greatly improves the efficiency and effect of the glucan in practical application, meets the requirement of industrial production for rapid dissolution, and has wide market prospects and application values.

Description

Technical Field

[0001] The invention relates to a method for improving the dissolution rate of beta-1,3 / alpha-1,3-glucan, and belongs to the field of preparations. Background Art

[0002] As a high-molecular-weight soluble polysaccharide composed of glucose as the basic unit connected by glycosidic bonds, dextran has a unique chemical structure and biological activity, which makes it show great application potential in many fields. It has biological activities such as lowering blood lipids, lowering blood sugar, anti-inflammatory, antioxidant and anti-tumor.

[0003] β-1,3 / α-1,3-glucan is made of sucrose as the main raw material through fermentation with Rhizobiumpusense, alcohol precipitation, filtration, separation, drying, and crushing. β-1,3 / α-1,3-glucan is a natural polysaccharide with a new structure. It is a repeating unit chain structure connected by 7 β-1,3-glycosidic bonds and 2 α-1,3-glycosidic bonds. It has no side chains. This unique bonding method not only enhances its molecular stability, but also gives it special physical and chemical properties and biological activity. Compared with traditional glucan, β-1,3 / α-1,3-glucan is a natural fully water-soluble β-glucan, so it is regarded as a new generation of food raw materials with great potential. Although its solubility is relatively superior, due to its relatively large molecular weight, this glucan shows a certain viscosity in the solution, which may constitute a certain limitation in some application products that pursue dissolution speed and instant solubility.

[0004] At present, there are many methods to try to improve the dissolution rate of glucan. For example, patent application number CN201611186178.5 proposes a method for degrading β-glucan using ultrasonic technology and enzymatic hydrolysis. Although the dissolution rate is improved, the structure of glucan is inevitably destroyed, affecting its biological activity. Another patent application number CN201310003621.0 significantly improves the solubility and dissolution rate of yeast β-D-glucan by using ionic liquid dissolution combined with microfluidization technology. However, this technology is difficult to achieve industrial production and is costly. In addition, patent application number CN201880000846.1 increases the dissolution rate of macromolecular polysaccharides by adding a high proportion of trehalose or erythritol, but this will reduce the purity of the polysaccharide, which is also not conducive to the wide application of the product. Although the above method improves the solubility of glucan to a certain extent, there are still many shortcomings. Although ultrasonic and enzymatic hydrolysis methods are effective, they will destroy the structure of glucan and affect its biological activity and stability; although ionic liquid dissolution combined with microfluidics has significant effects, the process is complex and costly, making it difficult to promote to large-scale production; and the method of adding excipients in high proportions reduces the purity of the product, which is not conducive to the market acceptance of the product. Therefore, there is an urgent need for a method that can significantly increase the dissolution rate of glucan while maintaining its structure and purity. This is of great significance for broadening the application field of glucan and increasing the added value of products.

[0005] In view of the above problems, the present invention proposes an innovative method for improving the dissolution rate of glucan granule preparations, in which β-1,3 / α-1,3-glucan is prepared into granule preparations to improve its dissolution rate. There is no related research at present, and the method is simple and easy to achieve efficient industrialization. The present invention provides us with a new and efficient way to apply glucan. Summary of the invention

[0006] The technical solution of the present invention is to provide a method for improving the dissolution rate of β-1,3 / α-1,3-glucan.

[0007] The present invention provides a method for improving the dissolution rate of β-1,3 / α-1,3-glucan, which comprises the following steps:

[0008] a. Take β-1,3 / α-1,3-glucan raw material, spray the adhesive, mix well, moisten, and prepare soft material;

[0009] b. Pass the soft material prepared in step a through a 30-40 mesh sieve and granulate;

[0010] c. Dry to obtain β-1,3 / α-1,3-glucan particles.

[0011] Wherein, in step a, the amount of adhesive used per gram of β-1,3 / α-1,3-glucan raw material is 0.5-0.55 ml.

[0012] Wherein, in step a, the amount of adhesive used per gram of β-1,3 / α-1,3-glucan raw material is 0.55 ml.

[0013] Wherein, the adhesive is a 50% ethanol solution.

[0014] Wherein, the soft material described in step b is sieved through a 40-mesh sieve.

[0015] Wherein, the drying method is constant temperature drying.

[0016] The method of the present invention achieves rapid dissolution of the glucan granule preparation by accurately controlling the preparation process of the glucan granules, screening and determining the optimal particle size range. This method not only maintains the biological activity and purity of the glucan, but also greatly improves its efficiency and effect in practical applications, meets the demand for rapid dissolution in industrial production, and has broad market prospects and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Dissolution time of dextran with different mesh sizes DETAILED DESCRIPTION

[0018] The β-1,3 / α-1,3-glucan raw materials used in the present invention were purchased from Sichuan Hetai Xinguang Biotechnology Co., Ltd. The method for determining the dissolution effect of β-1,3 / α-1,3-glucan in the embodiments of the present invention is as follows: 1 g of β-1,3 / α-1,3-glucan powder (granules) is added to the same volume of water at the same temperature, and the time required for complete dissolution is determined under the same stirring speed.

[0019] Example 1 Method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to the present invention

[0020] a. Take 60 g of β-1,3 / α-1,3-glucan raw material, spray it with 50% ethanol solution, mix well, moisten, and prepare a soft material;

[0021] b. Pass the soft material prepared in step a through a 40-mesh sieve and granulate;

[0022] c. Dry to obtain β-1,3 / α-1,3-glucan particles.

[0023] Example 2 Parameter screening test of the method for improving the dissolution rate of β-1,3 / α-1,3-glucan of the present invention

[0024] (i) Prepare sieves of 10 mesh, 20 mesh, 30 mesh, 40 mesh, 50 mesh and 60 mesh. β-1,3 / α-1,3-glucan raw material and adhesive.

[0025] (ii) Preparation of soft material: Take 60 g of β-1,3 / α-1,3-glucan raw material and spray the adhesive with a spray bottle until the raw material is in a state where it can be easily kneaded into a ball and easily dispersed when pressed.

[0026] In the process of spraying alcohol to make granules, the amount of wetting agent ethanol needs to be controlled. Too little ethanol will lead to incomplete granulation and a large amount of powder will remain after granulation. Too much ethanol will lead to soft or shapeless granules. The raw materials must be in a state where they can be easily kneaded into a ball and easily dispersed when pressed.

[0027] (III) Making wet granules: The soft material soaked with the adhesive is sieved through 60 mesh, 50 mesh, 40 mesh, 30 mesh, 20 mesh and 10 mesh sieves respectively to make granules for later use. During the granulation process, ensure that all raw materials are granulated and sieved.

[0028] (iv) Drying of wet granules: After sieving and granulating, the granules are placed in an oven and dried to a constant weight.

[0029] (V) Dissolution experiments were conducted on untreated raw material powder and preparation granules. 1 g of β-1,3 / α-1,3-glucan powder (granules) was added to the same volume of water at the same temperature, and the time required for complete dissolution was measured under the same stirring speed conditions. The results are shown in Table 1.

[0030] Table 1 Dissolution time of dextran with different mesh sizes

[0031]

[0032] Note: The same letters in the same column indicate no significant difference (P>0.01), and different letters indicate significant difference (P<0.01)

[0033] From Table 1 and Figure 1 It can be seen that the fastest dissolution rate is achieved when the particle size of β-1,3 / α-1,3-glucan is 40 mesh, which is significantly faster than that of untreated raw materials, and the dissolution rate is increased by more than 70%. From 10 mesh to 40 mesh, as the mesh number increases, that is, the particle size decreases, the dissolution rate increases significantly. However, from 40 mesh to 60 mesh, as the particle size decreases, the dissolution rate slows down significantly, indicating that the smaller the particle size, the better.

Claims

1. A method for improving the dissolution rate of β-1,3 / α-1,3-glucan, characterized in that: It includes the following steps: a. Take β-1,3 / α-1,3-glucan raw material, spray the adhesive, mix well, moisten, and prepare soft material; b. Pass the soft material prepared in step a through a 30-40 mesh sieve and granulate; c. Dry to obtain β-1,3 / α-1,3-glucan particles.

2. The method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to claim 1, characterized in that: In step a, the amount of binder used per gram of β-1,3 / α-1,3-glucan raw material is 0.5-0.55 ml.

3. The method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to claim 2, characterized in that: In step a, the amount of binder used per gram of β-1,3 / α-1,3-glucan raw material is 0.55 ml.

4. The method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to any one of claims 1 to 3, characterized in that: The adhesive is a 50% ethanol solution.

5. The method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to claim 1, characterized in that: The soft material described in step b is passed through a 40-mesh sieve.

6. The method for improving the dissolution rate of β-1,3 / α-1,3-glucan according to claim 1, characterized in that: The drying method is oven drying.

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