Core-shell type chromatographic stationary phase material as well as preparation method and application thereof

By coating the covalent organic framework materials with amide groups on silica microspheres, the problem of difficulty in separation of active polysaccharides is solved, and efficient separation of hydrophilic oligosaccharides and Morinacea oligosaccharides is achieved. Excellent separation performance and stability are demonstrated.

CN119259010APending Publication Date: 2025-01-07SHANXI UNIV
View PDF 0 Cites 1 Cited by

Patent Information

Application Number
CN202411593082.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-08
Publication Date
2025-01-07

AI Technical Summary

Technical Problem

The existing methods of separation and enrichment of active ingredients in traditional Chinese medicine are lagging behind, especially the difficulty in isolating degradation products caused by different monosaccharide composition and structure of polysaccharides, and the lack of rapid and effective separation of active units.

Method used

Using core-shell chromatography stationary phase material, a covalent organic frame material coated with amide groups on spherical silica is used to improve the separation performance of hydrophilic oligosaccharides by using electrostatic and π-π interactions. The specific steps include Preparation of silica microsphere modification and oxidation reaction of covalent organic framework.

Benefits of technology

It has achieved efficient separation of hydrophilic oligosaccharides and Morinacea oligosaccharides, with good separation performance, simple preparation process and good reproducibility and stability.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure HDA0005126094440000011
    Figure HDA0005126094440000011
  • Figure HDA0005126094440000012
    Figure HDA0005126094440000012
  • Figure HDA0005126094440000021
    Figure HDA0005126094440000021
Patent Text Reader

Abstract

The invention relates to a core-shell type chromatographic stationary phase material. The core-shell type chromatographic stationary phase material comprises spherical silicon dioxide and a covalent organic framework which coats the spherical silicon dioxide and contains an amide group. The preparation method comprises the following steps: firstly preparing modified silicon dioxide microspheres, then coating the surfaces of the silicon dioxide microspheres with a covalent organic framework through surface in-situ growth, and finally modifying the self-polycondensation covalent organic framework through oxidation reaction to obtain the self-polycondensation covalent organic framework material containing amide groups. The pore diameter of the material is 5-15 nanometers of mesopores, hydrophilic oligosaccharide substances can be separated, radix morindae officinalis oligosaccharide can be separated, and the material has good separation performance. The preparation steps are simple, the reaction conditions are mild, the method is simple and convenient, and good preparation reproducibility and stability are achieved.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Cited By

  • Monodispersed TPQD-coated SiO2 reversed-phase high-performance liquid chromatography stationary phase as well as preparation and application thereof

    CN121016690A