Device for microfluidic mixing

Abstract

The invention relates to the field of microfluidics, and discloses a device for microfluidics uniform mixing, the device comprises a kit and a sampling piece, the kit is provided with a placing cavity, the sampling piece is in sliding connection with the placing cavity through a transmission assembly, the sampling piece is provided with a sampling hole, and the sampling hole is communicated with the placing cavity. A sampling hole is formed in the kit, the sampling hole can be communicated with the placing cavity through the transmission assembly, the kit is provided with a sample adding opening, a detection area and a plurality of reaction cavities, and the sample adding opening, the detection area and the plurality of reaction cavities are communicated with the placing cavity; according to the device for micro-fluidic mixing, quantitative sampling of a sample is carried out through the sampling holes formed in the sampling piece, upward spraying type mixing of the sample and a reagent is achieved in combination with liquid path flowing on a kit, the shearing force on cells or other biological samples can be reduced by adopting upward spraying type mixing, and the integrity and activity of the samples can be kept; meanwhile, corresponding mechanisms such as a stirring rod do not need to be added, and the product structure is simplified.

Description

Technical Field

[0001] The present application relates to the technical field of microfluidics, and in particular to a device for microfluidic mixing. Background Art

[0002] Microfluidic cell mixing is a technology that utilizes microchannels and microfluidic control. Microfluidic cell mixing technology is mainly used in biomedical research, drug screening, cell analysis, and biomanufacturing. Its advantage is that it can efficiently and accurately control the speed, time, and degree of cell mixing. At the same time, it can operate on a microscale, saving the use of samples and reagents and improving experimental efficiency.

[0003] However, existing microfluidic mixing usually uses a rotation mixing method. The existing rotation mixing requires the addition of a stirring rod driven by a motor to mix, which increases the cost and is not suitable for some projects. The stirring process will break up the cells and affect the cell count.

[0004] Therefore, there is an urgent need for a device for microfluidic mixing to solve the above problems.

[0005] The above contents are only used to assist in understanding the technical solution of the present application and do not constitute an admission that the above contents are prior art. Summary of the invention

[0006] The main purpose of the present application is to provide a device for microfluidic mixing, aiming to solve the problem that the existing method of mixing cells using a stirring rod easily breaks the cells during the mixing process, thereby affecting the detection efficiency and detection results.

[0007] To achieve the above-mentioned purpose, a device for microfluidic mixing includes a reagent kit and a sampling sheet, wherein the reagent kit is provided with a placement cavity, the sampling sheet is slidably connected to the placement cavity through a transmission component, a sampling hole is provided on the sampling sheet, and the sampling hole can be connected to the placement cavity through the transmission component. The reagent kit is provided with a sample addition port, a detection area, and a plurality of reaction chambers, and the sample addition port, the detection area, and the plurality of reaction chambers are connected to the placement cavity.

[0008] As a preferred solution of the present application, it further comprises a sample adding channel, one end of the sample adding channel is connected to the sample adding port, and the other end of the sample adding channel is connected to the placement cavity.

[0009] As a preferred embodiment of the present application, the reaction chamber includes a reagent chamber for storing reagents and a mixing chamber for mixing added samples with reagents, one end of the reagent chamber is connected to a first air channel, and the other end of the reagent chamber is connected to a placement chamber through a reagent channel, one end of the mixing chamber is connected to a second air channel, and the other end of the mixing chamber is connected to the placement chamber through a mixing channel.

[0010] As a preferred embodiment of the present application, it further comprises a waste liquid collection area and a waste liquid channel, one end of the waste liquid channel is connected to the waste liquid collection area, and the other end of the waste liquid channel is connected to the reagent kit.

[0011] As a preferred solution of the present application, it further includes a detection channel, one end of which is connected to the detection area, and the other end of which is connected to the placement cavity.

[0012] As a preferred solution of the present application, a plurality of protrusions are provided on the sampling piece body, and the plurality of protrusions are used for interference connection with the placement cavity.

[0013] As a preferred solution of the present application, the test kit includes a card body, a front cover plate and a rear cover plate, wherein the front cover plate is connected to one side of the card body, and the rear cover plate is connected to the other side of the card body.

[0014] As a preferred solution of the present application, it also includes a sample flow channel. A sample flow channel is provided on one side of the card body close to the rear cover plate, and the sample flow channel is used for the flow of the sample itself.

[0015] As a preferred solution of the present application, a reserved channel is further included. The reagent kit is provided with the reserved channel, one end of the reserved channel is connected to the placement cavity, and the other end of the reserved channel is connected to the mixing channel.

[0016] As a preferred solution of the present application, the shape of the sample addition port is an inverted cone structure.

[0017] The present application provides a device for microfluidic mixing, which performs quantitative sampling of samples through sampling holes opened on a sampling sheet, and combines the liquid flow on a test kit to achieve upward spray mixing of samples and reagents. The upward spray mixing can reduce the shear force on cells or other biological samples, which is beneficial to maintaining the integrity and activity of the samples and avoiding cell breakage; the mixing effect is very good; at the same time, since there is no need to add corresponding mechanisms such as stirring rods, the product structure is simplified, and the stirring rod is prevented from breaking cells during the stirring process, affecting the experimental test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Schematic diagram of the three-dimensional structure of the device used for microfluidic mixing in this application; Figure 2 An exploded view of a device for microfluidic mixing in this application; Figure 3 This is a schematic diagram of the first three-dimensional structure of the card body in the device for microfluidic mixing in this application; Figure 4Schematic diagram of the three-dimensional structure of the sampling piece in the device for microfluidic mixing in this application; Figure 5 This is a schematic diagram of a second three-dimensional structure of a card body in the device for microfluidic mixing in this application; Figure 6 It is a cross-sectional view of the card body in the device for microfluidic mixing in this application.

[0019] Description of reference numerals: 1. Card body; 2. Front cover; 3. Back cover; 4. Sample piece; 101. placement cavity; 102. sample loading port; 103. reagent cavity; 104. mixing cavity; 105. detection area; 106. waste liquid collection area; 107. sample loading channel; 108. first air channel; 109. reagent channel; 110. second air channel; 111. mixing channel; 112. reserved channel; 113. detection channel; 114. sample flow channel; 115. waste liquid channel; 401. protrusion; 402. sampling hole. DETAILED DESCRIPTION

[0020] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0021] In addition, if the descriptions of "first", "second", etc. are involved in this application, they are only used for descriptive purposes (such as for distinguishing the same or similar elements), and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various implementation examples can be combined with each other, but they must be based on the ability of ordinary technicians in this field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0022] Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4In one embodiment, a device for microfluidic mixing includes a reagent kit and a sampling sheet 4, wherein a placement cavity 101 is provided on the reagent kit, and the sampling sheet 4 is slidably connected to the placement cavity 101 through a transmission component, and a sample addition port 102, a detection area 105, and a plurality of reaction cavities are provided on the reagent kit, and the sample addition port 102, the detection area 105, and the plurality of reaction cavities are connected to the placement cavity 101, and a sampling hole 402 is provided on the sampling sheet 4, and the sampling hole 402 can be connected to the placement cavity 101 through a transmission component, and the transmission component is used to transmit the sample. The moving component pushes the sampling piece 4, so that the sampling hole 402 moves to the position of the channel inlet or channel outlet opened on the placement cavity 101, and the sampling hole 402 is in a coaxial state with the channel inlet or channel outlet. At this time, the sampling hole 402 is connected with each channel on the placement cavity 101, and the liquid sample is added to the sample port 102 to make the sample enter the sampling hole 402 of the sampling piece 4. The fixed volume of the sampling hole 402 is used to complete the accurate quantitative sampling of the sample, and then the sample is mixed and tested.

[0023] For details, please refer to Figure 3 In this embodiment, the transmission assembly is composed of a screw motor, a guide assembly, a push rod and a position sensor. The position sensor and the screw motor are electrically connected to a control system (not shown in the figure) to control the screw motor. The screw motor is connected to the push rod, and the push rod is driven to move by the screw motor, so that the push rod pushes the sample piece 4 to slide on the placement cavity 101. The precise movement of the sample piece 4 can be achieved through the transmission of the position sensor and the push rod.

[0024] For details, please refer to Figure 3 In the present embodiment, the shape of the sample adding port 102 is an inverted cone structure. It can be understood that the inverted cone design can reduce the sample residue in the sample adding port 102, avoid cross contamination or mixing caused by residual samples, and at the same time, it is compatible with TIP heads of different specifications to ensure the sealing when the TIP head is used for sample addition. The inverted cone design makes the edge of the sample adding port 102 narrower, which is convenient for accurately adding a small volume of sample, helps to avoid sample waste and improve operation accuracy.

[0025] For details, please refer to Figure 3 , Figure 4 In this embodiment, a sample loading channel 107 is further included. The sample loading channel 107 is arranged on the reagent box at an angle. One end of the sample loading channel 107 is connected to the sample loading port 102, and the other end of the sample loading channel 107 is connected to the placement cavity 101. It can be understood that the sample added from the sample loading port 102 flows into the sampling sheet 4 along the sample loading channel 107, and the quantitative sampling of the sample is completed through the fixed volume of the sampling hole 402 on the sampling sheet 4, so that accurate quantification of the sample can be achieved.

[0026] For details, please refer to Figure 3In this embodiment, the reaction chamber includes a reagent chamber 103 for storing reagents and a mixing chamber 104 for mixing the added sample with the reagent. One end of the reagent chamber 103 is connected to a first air channel 108, and the other end of the reagent chamber 103 is connected to the placement chamber 101 through a reagent channel 109. One end of the mixing chamber 104 is connected to a second air channel 110, and the other end of the mixing chamber 104 is connected to the placement chamber 101 through a mixing channel 111. It can be understood that the first air channel 108 and the second air channel 110 are connected to an air pump, and the first air channel 108 and the second air channel 110 are independently controlled by moving the sampling piece 4; the air pump is electrically connected to the control system, and the end of the second air channel 110 away from the air pump is connected to the top of the mixing chamber 104, and the mixing channel 111 is connected to the bottom of the mixing chamber 104. Air is introduced into the first air channel 108, and the reagent in the reagent chamber 103 drives the sample in the sampling hole along the reagent channel 109, the sampling hole 203, and the mixing channel 111 into the mixing chamber 104. Air is continuously injected into the first air channel 108 to fill the reagent chamber. The sample in the sample sheet 4 of the reagent kit in 103 is transferred to the mixing chamber 104, and the reagent enters the mixing chamber 104 from the bottom to form an upward spray-type mixing. When there is excess gas, the excess gas is blown into the mixing chamber 104 from the bottom through the mixing channel 111 to enhance the mixing effect, so that the sample and the reagent are mixed in the mixing chamber in an upward spray-type manner, which can reduce the shear force on cells or other biological samples, is beneficial to maintaining the integrity and activity of the sample, and avoids cell breakage. During the entire mixing process, there is no need to add a stirring rod to mix the sample and the reagent, which avoids the situation where the stirring rod is used to destroy the sample during the mixing process and affect the test results.

[0027] For details, please refer to Figure 5 In this embodiment, it also includes a waste liquid collection area 106 and a waste liquid channel 115. One end of the waste liquid channel 115 is connected to the waste liquid collection area 106, and the other end of the waste liquid channel 115 is connected to the reagent kit. It can be understood that when the sampling piece is in the preset position, the other end of the waste liquid channel 115 is connected to the sampling hole 402 on the sampling piece in the placement cavity 101. By setting the waste liquid channel 115, the excess sample added to the sampling port can be collected to avoid the leakage of excess sample and the pollution of the equipment and laboratory environment.

[0028] For details, please refer to Figure 5 , Figure 6In the present embodiment, a detection channel 113 is also included, one end of the detection channel 113 is connected to the detection area 105, and the other end of the detection channel 113 is connected to the placement cavity 101. It can be understood that by setting the detection area 105, a corresponding detection device can be set in the detection area 105 domain, and the mixed solution after the sample and the reagent are mixed can be detected, such as cell counting, turbidimetry, biochemical immunity, chemiluminescence and other detection items of the mixed solution. The overall structure is more compact and the function of the test kit is more complete.

[0029] For details, please refer to Figure 3 , Figure 4 In this embodiment, a plurality of protrusions 401 are provided on the body of the sampling piece 4, and the plurality of protrusions 401 are used for interference connection with the placement cavity 101; it can be understood that by connecting the protrusions 401 on the sampling piece 4 with the placement cavity 101, the surface of the protrusion 401 is in close contact with the placement cavity 101, which can effectively reduce the leakage of the sample from the sampling hole 402 during the movement of the sampling piece 4. At the same time, the protrusions on the sampling piece are used to control the opening and closing of the flow channel, thereby achieving sealing and reducing the friction between the sampling piece and the placement cavity, which is beneficial to improving the detection accuracy.

[0030] For details, please refer to Figure 2 In this embodiment, the test kit includes a card body 1, a front cover 2 and a rear cover 3. The front cover 2 is connected to one side of the card body 1, and the rear cover 3 is connected to the other side of the card body 1. It can be understood that through this structural design, it is convenient to process channels or flow channels on the card body 1, which can save production costs on the other hand.

[0031] For details, please refer to Figure 5 In the present embodiment, a sample flow channel 114 is also included. The sample flow channel 114 is provided on one side of the card body 1 close to the rear cover 3. The sample flow channel 114 is used for the flow of the sample itself. It can be understood that by providing the sample flow channel 114, the circulation of samples, reagents and a mixed solution consisting of samples and reagents can be achieved. At the same time, it is also convenient to serve as an auxiliary flow channel for the sampling piece 4 to realize the opening and closing functions of the gas and liquid paths.

[0032] For details, please refer to Figure 2 , Figure 3 , Figure 6In the present embodiment, a reserved channel 112 is further included. The reagent kit is provided with the reserved channel 112. One end of the reserved channel 112 is communicated with the placement cavity 101, and the other end of the reserved channel 112 is communicated with the mixing channel 111. It can be understood that the other end of the reserved channel 112 can be communicated with the detection channel 113 through the sampling hole 402 on the sampling sheet 4. At this time, the protrusion 401 on the sampling sheet 4 blocks the sample flow channel 114, and the sample and reagent mixed liquid in the mixing cavity 104 is transported to the detection area 105 through the mixing channel 111 and the reserved channel 112, the sampling hole 402 and the detection channel 113 to complete the detection of the mixed liquid.

[0033] In summary, in the present application, the sample is added to the sample adding port 102, and the sample enters the sampling hole 402 of the sampling sheet 4 along the sample adding channel 107. Under the drive of the transmission component, the push rod pushes the sampling sheet 4 to move. When the sampling hole 402 containing the sample moves to be coaxial with the center point of the outlet position of the reagent channel 109, the sampling sheet 4 stops moving. At this time, gas is introduced into the first gas channel 108 to drive the reagent pre-added in the reagent chamber 103 to flow along the reagent channel 109. The reagent carries The quantitatively measured sample moves along the sample channel into the mixing channel 111, and then enters the mixing chamber 104 together to achieve upward spray mixing. Under the driving of the transmission component again, the sampling piece 4 continues to move. At this time, the reserved channel 112 is connected with the sampling piece 4. By passing gas into the second airway 110, the mixed solution of the sample and the reagent enters the sampling hole 402 of the sampling piece 4 along the reserved channel 112, and then flows into the detection area 105 through the detection channel 113 to complete the detection of the mixed solution. A device for microfluidic mixing can reduce the shear force on cells or other biological samples by adopting upward jet mixing, which is beneficial to maintaining the integrity and activity of the samples and avoiding cell breakage; the mixing effect is very good; at the same time, since there is no need to add corresponding mechanisms such as stirring rods, the product structure is simplified, and the stirring rod is prevented from breaking cells during the mixing process and affecting the experimental test results.

[0034] It should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, device, article or method including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, device, article or method. In the absence of further restrictions, an element defined by the sentence "includes a ..." does not exclude the presence of other identical elements in the process, device, article or method including the element.

[0035] The above description is only a preferred embodiment of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A device for microfluidic mixing, characterized in that: include: A test kit and a sampling sheet, wherein the test kit is provided with a placement cavity, the sampling sheet is slidably connected to the placement cavity via a transmission component, a sampling hole is opened on the sampling sheet, and the sampling hole can be connected to the placement cavity via the transmission component, the test kit is provided with a sample addition port, a detection area and a plurality of reaction chambers, and the sample addition port, the detection area and the plurality of reaction chambers are connected to the placement cavity.

2. The device for microfluidic mixing according to claim 1, characterized in that: It also includes a sample adding channel, one end of which is communicated with the sample adding port, and the other end of which is communicated with the placement cavity.

3. The device for microfluidic mixing according to claim 1, characterized in that: The reaction chamber includes a reagent chamber for storing reagents and a mixing chamber for mixing added samples with reagents, one end of the reagent chamber is connected to a first air channel, and the other end of the reagent chamber is connected to a placement chamber through a reagent channel, one end of the mixing chamber is connected to a second air channel, and the other end of the mixing chamber is connected to the placement chamber through a mixing channel.

4. The device for microfluidic mixing according to claim 1, characterized in that: It also includes a waste liquid collection area and a waste liquid channel, one end of the waste liquid channel is connected to the waste liquid collection area, and the other end of the waste liquid channel is connected to the reagent box.

5. The device for microfluidic mixing according to claim 1, characterized in that: It also includes a detection channel, one end of which is connected to the detection area, and the other end of which is connected to the placement cavity.

6. The device for microfluidic mixing according to claim 1, characterized in that: The sampling sheet body is provided with a plurality of protrusions, and the plurality of protrusions are used for interference connection with the placement cavity.

7. The device for microfluidic mixing according to claim 1, characterized in that: The test kit comprises a card body, a front cover plate and a rear cover plate, wherein the front cover plate is connected to one side of the card body, and the rear cover plate is connected to the other side of the card body.

8. The device for microfluidic mixing according to claim 7, characterized in that: It also includes a sample flow channel. The card body is provided with a sample flow channel on one side close to the rear cover plate, and the sample flow channel is used for the flow of the sample itself.

9. The device for microfluidic mixing according to claim 3, characterized in that: It also includes a reserved channel. The reagent kit is provided with the reserved channel. One end of the reserved channel is connected to the placement cavity, and the other end of the reserved channel is connected to the mixing channel.

10. The device for microfluidic mixing according to claim 2, characterized in that: The shape of the sample adding port is an inverted cone structure.

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