Antioxidant and anti-wrinkle effects and application of artemisia apiacea volatile oil

Abstract

The antioxidant effect and the anti-wrinkle effect of the artemisia apiacea volatile oil are proved by determining certain biochemical indexes (matrix metalloproteinase 1 and ROS content) of an irradiated skin model, and the artemisia apiacea volatile oil belongs to the fields of medicines and cosmetics; meanwhile, the invention provides a formula and a preparation process of various cosmetics containing the artemisia apiacea volatile oil. The invention not only provides theoretical support for the application of the artemisia apiacea volatile oil, but also provides an example of practical application.

Description

Technical Field

[0001] The invention relates to the fields of medicine and cosmetics, and in particular to the anti-oxidation and anti-wrinkle effects of Artemisia annua volatile oil and applications thereof. Technical Background

[0002] Artemisia annua is a traditional Chinese herbal medicine, in which artemisinin is an effective anti-malarial ingredient. In the process of extracting artemisinin from Artemisia annua for anti-malarial purposes, a large amount of Artemisia annua volatile oil (AN+) is not used and is wasted. The Artemisia annua volatile oil is an active ingredient obtained by further purifying the residual substances in the artemisinin extraction process. In recent years, research on Artemisia annua has mainly focused on artemisinin and its derivatives, but research on Artemisia annua volatile oil is relatively less.

[0003] In 2019, Shanghai Kunming Pharmaceutical Group conducted research on the application of Artemisia annua volatile oil in cosmetics, exploring the quality control, efficacy research, formula application and other properties of Artemisia annua volatile oil, and opened up a new direction for Artemisia annua volatile oil in the field of cosmetics. In vitro studies have shown that Artemisia annua volatile oil can upregulate the expression of skin barrier-related genes (filament aggregate protein gene FLG) to enhance the skin barrier and repair skin sensitivity; at the same time, it can inhibit the release of multiple inflammatory mediators (IL-6, IL-8 and TSLP) induced by lipopolysaccharide (LPS) and Staphylococcus aureus, and has anti-inflammatory and soothing effects [1]. Artemisia annua volatile oil can also alleviate the degree of skin lesions in mice (skin disease model) and reduce the inflammatory factors in mouse serum. It has certain therapeutic significance for skin diseases such as atopic dermatitis, skin barrier damage, and psoriasis [2-8]. In addition, human clinical trials have found that after Artemisia annua volatile oil is incorporated into skin care products, products such as lotions, essences, emulsions, and creams containing Artemisia annua volatile oil can relieve skin problems such as sensitivity and redness [9,10].

[0004] As the largest organ of the human body, the skin is exposed to the external environment for a long time and will inevitably be attacked by free radicals. Free radicals are highly active molecules that can destroy the structure of skin cells, causing damage to collagen and elastic fibers, thereby accelerating the aging process of the skin. This will not only cause the skin to become dry, rough, and lose elasticity, but also lead to the appearance of aging signs such as spots and wrinkles.

[0005] Antioxidation is an important part of skin care. Antioxidant ingredients help neutralize free radicals and reduce the damage to skin cells, thereby maintaining the health of the skin. For example, natural antioxidant ingredients such as vitamin E, vitamin C, and green tea extract can effectively remove free radicals and protect skin cells from oxidative stress. In addition, antioxidant ingredients can enhance the self-repair ability of the skin, promote the production of collagen, and improve the firmness and glossiness of the skin. Therefore, in daily skin care, choosing skin care products rich in antioxidant ingredients is crucial to maintaining the youthful state of the skin. They can not only help the skin resist the invasion of the external environment, but also delay the aging process of the skin, keeping the skin healthy, smooth and elastic. With the increase of age, the natural antioxidant capacity of the skin will gradually decrease. Therefore, timely supplementation of antioxidant ingredients and strengthening of the skin's antioxidant defense are particularly important for maintaining the health and beauty of the skin. With people's attention to naturalness and safety, the search for novel skin antioxidants in the cosmetics industry has never stopped, but at present, there is little research on the anti-oxidation of Artemisia annua volatile oil in skin. Artemisia annua volatile oil can effectively relieve skin inflammation and may have a certain antioxidant protective effect on the skin. Therefore, it is necessary to explore whether Artemisia annua volatile oil has the ability to resist skin oxidation.

[0006] In addition, anti-aging is also a trend in the current era of functional skin care. People's pursuit of anti-aging has prompted the cosmetics industry to continuously develop new anti-aging raw materials. Inflammation is part of the body's defense mechanism. When the immune system recognizes and eliminates internal or external harmful stimuli and begins to heal, it will cause inflammation; and aging is a common, complex, and naturally occurring process. As one of the twelve major signs of aging, chronic inflammation is inseparable from aging. The concept of inflammatory aging is extended from immune aging, that is, after the immune system degenerates, it cannot clear senescent cells and pro-inflammatory factors in time, and the level of inflammation will increase accordingly; and when low-level chronic inflammation gradually accumulates, it will further aggravate aging and form a vicious circle. Artemisia annua volatile oil has good anti-inflammatory effects, so it is also necessary to explore whether Artemisia annua volatile oil has certain skin anti-aging effects. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the research on Artemisia annua volatile oil, the present invention confirms the antioxidant and anti-wrinkle effects of Artemisia annua volatile oil by measuring some biochemical indicators (matrix metalloproteinase 1 and ROS content) of the irradiated skin model, belonging to the field of medicine and cosmetics; and provides a variety of formulas and preparation processes for cosmetics containing Artemisia annua volatile oil. The present invention not only provides theoretical support for the application of Artemisia annua volatile oil, but also provides examples of practical application.

[0008] In one aspect, the present invention provides a use of Artemisia annua volatile oil for preparing a preparation for improving the antioxidant effect of the product.

[0009] Artemisia annua volatile oil is further processed from the "waste" generated during the production of artemisinin. Because of its superior anti-inflammatory function, it is widely used in the fields of skin care and antibacterial. However, it is still unknown whether Artemisia annua volatile oil has other functions. The exploration of the functions of Artemisia annua volatile oil can not only provide a certain theoretical basis for its application, but also increase the economic benefits of the Artemisia annua processing industry. The present invention evaluates the antioxidant effect of Artemisia annua volatile oil by detecting the level of active oxygen in an in vitro skin model after irradiation. The active oxygen broadly refers to free radicals and non-free radicals of oxygen origin, including superoxide anions (O2 - ), hydrogen peroxide (H2O2), hydroxyl radical (OH - ), ozone (O3) and singlet oxygen ( 1 O2), because they contain unpaired electrons, they have high chemical reactivity and can cause irreversible damage to the skin barrier. The results showed that Artemisia annua volatile oil can significantly inhibit the generation of reactive oxygen in the skin, and the effect is better than the positive control vitamin E. Vitamin E is a recognized antioxidant with high reducing properties and is often added to skin care products. This result fully proves that Artemisia annua volatile oil has excellent antioxidant function, which may be because it contains antioxidant components such as camphor, camphene, and eucalyptol.

[0010] Furthermore, the concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v), preferably 3%.

[0011] Furthermore, the antioxidant is Artemisia annua volatile oil that inhibits the generation of active oxygen.

[0012] Furthermore, the products include but are not limited to facial cream, eye cream, lotion, essence, toner, gel, facial mask, facial cleanser, facial honey, facial foam, sunscreen, shower gel, spray, etc.

[0013] In another aspect, the present invention provides a use of Artemisia annua volatile oil for preparing a preparation for improving the anti-wrinkle effect of the product.

[0014] The present invention evaluates the anti-wrinkle effect of Artemisia annua volatile oil by detecting the matrix metalloproteinase 1 (MMP-1) of the in vitro skin model after irradiation. The main function of MMP-1 is to degrade matrix proteins. It degrades matrix molecules such as collagen fibronectin and prothrombin by cutting the peptide bonds of matrix proteins. Therefore, the higher the content of MMP-1, the more collagen fibers in the skin are degraded, and the skin is more prone to wrinkles. The results show that Artemisia annua volatile oil can significantly reduce the content of matrix metalloproteinase 1 in the skin, and the effect is better than that of the positive control dexamethasone. The results fully prove that Artemisia annua volatile oil has excellent anti-wrinkle effect, which may be because it contains components such as artemisinone and β-caryophyllene.

[0015] Furthermore, the concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v), preferably 3%.

[0016] Furthermore, the anti-wrinkle effect is that the Artemisia annua volatile oil downregulates the content of matrix metalloproteinase 1.

[0017] Furthermore, the products include but are not limited to facial cream, eye cream, lotion, essence, toner, gel, facial mask, facial cleanser, facial honey, facial foam, sunscreen, shower gel, spray, etc.

[0018] On the other hand, the present invention provides a use of Artemisia annua volatile oil to improve the antioxidant effect of products by inhibiting the generation of active oxygen.

[0019] On the other hand, the present invention provides a use of Artemisia annua volatile oil to improve the anti-wrinkle effect of a product by down-regulating the content of matrix metalloproteinase 1.

[0020] On the other hand, the present invention provides a composition for preparing an agent for improving the antioxidant and / or anti-wrinkle effect of a product, characterized in that the composition comprises at least one of Artemisia annua volatile oil, acetyl hexapeptide-8, and palmitoyl tripeptide-5.

[0021] Furthermore, the composition is a combination of Artemisia annua volatile oil + acetyl hexapeptide-8, a combination of Artemisia annua volatile oil + palmitoyl tripeptide-5, a combination of acetyl hexapeptide-8 + palmitoyl tripeptide-5, and a combination of Artemisia annua volatile oil + acetyl hexapeptide-8 + palmitoyl tripeptide-5.

[0022] The present invention confirms that among the above combinations, except for the combination of acetyl hexapeptide-8+palmitoyl tripeptide-5, the other combinations have synergistic effects in anti-oxidation and anti-wrinkle, and the combination containing three of the chemical substances has the best effect; when the composition has only two groups of chemical components, the combination of Artemisia annua volatile oil+acetyl hexapeptide-8 is preferred to improve the antioxidant effect of the product, and the combination of Artemisia annua volatile oil+palmitoyl tripeptide-5 is preferred in terms of anti-wrinkle.

[0023] The beneficial effects of the present invention include:

[0024] 1. The present invention confirms that Artemisia annua volatile oil can significantly inhibit the generation of reactive oxygen species in a 3D epidermal skin model, with a downregulation rate of 84.88%, which is better than the effect of the positive control vitamin E, indicating that Artemisia annua volatile oil has a superior antioxidant effect;

[0025] 2. The present invention confirms that Artemisia annua volatile oil can significantly inhibit the production of matrix metalloproteinase 1 (MMP-1) in a 3D epidermal skin model, with a downregulation rate of 50.80%, which is better than the effect of the positive control dexamethasone, indicating that Artemisia annua volatile oil has excellent anti-wrinkle effect;

[0026] 3. The present invention proves that the combination of Artemisia annua volatile oil, acetyl hexapeptide-8 and / or palmitoyl tripeptide-5 has a synergistic effect in anti-oxidation and anti-wrinkle. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 : ROS fluorescence results in the skin model after radiation; the picture was taken with a fluorescence microscope (Olympus, BX43) at a magnification of 20×. The green fluorescence is the ROS signal. The stronger the green fluorescence intensity, the more ROS content.

[0029] Figure 2 : Bar graph of relative integrated optical density (IOD) values ​​of ROS in the skin model after radiation;

[0030] Figure 3 : Bar graph of MMP-1 concentration detection in irradiated skin model. DETAILED DESCRIPTION

[0031] The present invention is further elaborated in detail below in conjunction with the accompanying drawings and specific embodiments of the specification. The embodiments are only used to explain the present invention and are not used to limit the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention.

[0032] The Artemisia annua volatile oil described in the present invention is prepared according to the Artemisia annua volatile oil preparation method described in Chinese patents CN116725905A, CN116725906A, CN116725907A, and CN116731785A. The test experiments of the present invention are all based on the 3D epidermal skin model. The present invention is based on the "Method for Detecting the Content of Matrix Metalloproteinase 1 (MMP-1) and Reactive Oxygen Species (ROS)". Unless otherwise specified, the test methods used in the following examples are all conventional methods.

[0033] The model used in the present invention is a 3D epidermal skin model Batch number: ES240901, provided by Guangdong Boxi Biotechnology Co., Ltd.; the reagents used are as follows: EpiGrowth culture medium (Guangdong Boxi Biotechnology), PBS (Solaibao), dexamethasone (China Food and Drug Administration), vitamin E (VE, Sigma), paraformaldehyde (Biosharp), MMP-1-ELISA kit (Abcam), ROS kit (Biosharp), DCFH-DA probe (Biosharp), acetyl hexapeptide-8 (Shandong Jipeptide Biotechnology Co., Ltd.), palmitoyl tripeptide-5 (Shandong Jipeptide Biotechnology Co., Ltd.); the equipment used are as follows: CO2 incubator (Thermo, 150I), clean bench (Sujing Antai, SW-CJ-2F), UVB irradiator (Philips), fluorescence microscope (Olympus, BX43), microplate reader (BioTek, Epoch); the other materials and reagents used, unless otherwise specified, are reagents and materials that can be obtained from commercial channels.

[0034] Example 1: Antioxidant function of Artemisia annua volatile oil

[0035] In this example, the antioxidant effect of the sample to be tested is evaluated by detecting the change in the content of reactive oxygen species (ROS). The parameters of each group are shown in Table 1.

[0036] Table 1 Groups and parameter settings for the test of the antioxidant function of Artemisia annua volatile oil The specific steps of ROS determination are as follows:

[0037] 1.1 Drug administration and irradiation

[0038] 1) According to the test grouping (Table 1), the 3D epidermal skin model was transferred to a 6-well plate, and the test group number was marked on the 6-well plate, and 0.9 mL of EpiGrowth culture medium was added to each well of the well plate in advance;

[0039] 2) Model administration: According to the test groups, the BC group did not receive any treatment, the PC1 group received VE (final concentration of 7 μg / mL) in the culture medium (0.9 mL); the sample group received VE at 2 mg / cm 2 3% of Artemisia annua volatile oil was applied to the surface of the skin model, the area of ​​the skin model was 0.64 cm 2 .

[0040] 3) UVB irradiation: Except for the BC group, all other groups were irradiated with UVB, and the irradiation dose was 600mJ / cm 2 .

[0041] 1.2 Reactive oxygen species (ROS) test

[0042] 1) Probe incubation: After irradiation, add 3.7 mL of 20 μM probe working solution to each well and incubate in a CO2 incubator (37°C, 5% CO2) for 1 h. Shake the well plate every 20 min to ensure uniform incubation of the model.

[0043] 2) Sample collection: After the incubation is completed, the model is cut and placed in a 1.5 mL centrifuge tube. 1 mL of 4% paraformaldehyde fixative is added to the tube, wrapped with tin foil, and frozen sections are made;

[0044] 3) Washing and sealing the slices: Wash the cut frozen slices with PBS buffer for 8 minutes; after washing, wipe the surrounding liquid with absorbent paper, drop a small drop (about 20 μL) of fluorescent anti-quenching sealing agent on the slices with a 200 μL pipette tip, and seal the slices with a cover glass;

[0045] 4) Take pictures: Observe with a fluorescence microscope and take fluorescent pictures.

[0046] 1.3 Calculation of Downward Adjustment Rate

[0047] According to the formula The down-regulation rate of ROS content was calculated.

[0048] 1.4 Statistical analysis of results

[0049] The ROS fluorescence integrated optical density was calculated using IPP statistics and plotted using GraphPad Prism. The results were expressed as Mean±SD. The t-test was used for comparison among the groups, and the statistical analysis was two-tailed. P<0.05 represented a significant difference, and P<0.01 represented an extremely significant difference. For specific results, see Figures 1-2 and Table 2.

[0050] Table 2 Statistical results of ROS fluorescence density

[0051]

[0052] Note: The integrated optical density (IOD) reflects the content of ROS. When the t-test method is used for statistical analysis, compared with the BC group, the significance is represented by #, that is, P-value < 0.05 is represented by #, and P-value < 0.01 is represented by ##; compared with the NC group, the significance is represented by *, that is, P-

[0053] P-value<0.05 is indicated as *, P-value<0.01 is indicated as **

[0054] Compared with the BC group, the ROS content in the NC group increased significantly, indicating that the stimulation conditions of this test were effective; compared with the NC group, the ROS content in the PC1 group decreased significantly, indicating that the positive control of this test was effective; compared with the NC group, the ROS content of Artemisia annua volatile oil decreased significantly, that is, the down-regulation rate was 84.88%, and its inhibitory effect was better than that of the positive control, about 5% higher, indicating that the antioxidant effect of Artemisia annua volatile oil is excellent.

[0055] Example 2: Anti-wrinkle effect of Artemisia annua volatile oil

[0056] In this embodiment, UVB is used to irradiate the 3D epidermal skin model. The anti-wrinkle efficacy of the samples was evaluated by detecting the changes in the content of matrix metalloproteinase 1 (MMP-1). The parameters of each group are shown in Table 3. Dexamethasone is a glucocorticoid with a wide range of biological effects, including anti-inflammatory and immunosuppressive effects. In cell and molecular biology studies, dexamethasone has been shown to upregulate the expression of MMPs in certain cell types, including MMP-1. Therefore, dexamethasone was used as a positive control to verify the effectiveness of the experimental system.

[0057] Table 3 Groups and parameter settings for the anti-wrinkle efficacy test of Artemisia annua volatile oil

[0058]

[0059]

[0060] The specific steps for detecting the content of matrix metalloproteinase 1 are as follows:

[0061] 2.1 Drug administration and irradiation

[0062] 1) According to the test grouping (Table 3), the model was transferred to a 6-well plate, 0.9 mL of EpiGrowth culture medium was added to each well of the plate in advance, and the test group number was marked on the 6-well plate;

[0063] 2) Model administration: According to the test groups, the BC group did not receive any treatment, the PC2 group received 0.01% dexamethasone in the culture medium (0.9 mL); the sample group received 2 mg / cm 2 3% of Artemisia annua volatile oil was applied to the surface of the skin model, the area of ​​the skin model was 0.64 cm 2

[0064] 3) UVB irradiation: Except for the BC group, all other groups were irradiated with UVB, and the irradiation dose was 600mJ / cm 2 , the 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 h;

[0065] 4) After the incubation, the residual test substance was washed with a sterile PBS (pH=7.4) solution, and the residual liquid was wiped off with a sterile cotton swab.

[0066] 2.2ELISA test

[0067] After the incubation, the 3D epidermal skin model culture medium was collected in a 1.5 mL centrifuge tube and stored in a -80°C refrigerator. The detection and analysis were performed according to the operating instructions of the ELISA kit (Abcam).

[0068] 2.3 Calculation of inhibition rate

[0069] According to the formula The inhibition rate was calculated.

[0070] 2.4 Statistical analysis of results

[0071] GraphPad Prism was used for drawing, and the results were expressed as Mean ± SD. The t-test was used for statistical analysis among the groups, and all statistical analyses were two-tailed. P < 0.05 indicated a significant difference, and P < 0.01 indicated an extremely significant difference. The results are shown in Table 4 and Figure 3 shown.

[0072] Table 4 Detection results of MMP-1 content

[0073]

[0074] Note: When using t-test for statistical analysis, compared with the BC group, the significance is indicated by #, that is, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, that is, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by

[0075] **

[0076] Compared with the BC group, the MMP-1 content in the NC group increased significantly, indicating that the stimulation conditions of this test were effective; compared with the NC group, the MMP-1 content in the PC2 group decreased significantly, indicating that the positive control of this test was effective; compared with the NC group, the MMP-1 content of 3% Artemisia annua volatile oil decreased significantly, with an inhibition rate of 50.80%, and its inhibition effect was better than that of the positive control, about 22% higher, indicating that the anti-wrinkle effect of Artemisia annua volatile oil is excellent.

[0077] Application Example 1: Preparation method of facial cream containing Artemisia annua volatile oil

[0078] This application example provides a method for preparing a facial cream containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial cream is shown in Table 5.

[0079] Table 5 Formula of cream containing Artemisia annua volatile oil

[0080]

[0081] The preparation process of the facial cream is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize for 3-10 minutes with appropriate homogenization strength; stir and cool to 50°C, add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, and cool to 37°C before discharging.

[0082] Application Example 2: Preparation method of eye cream containing Artemisia annua volatile oil

[0083] This application example provides a method for preparing an eye cream containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the eye cream is shown in Table 6.

[0084] Table 6 Formula of eye cream containing Artemisia annua volatile oil

[0085]

[0086] The preparation process of the eye cream is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize for 3-10 minutes with appropriate homogenization strength; add phase C, homogenize for 3 minutes until uniform, stir and cool to 50°C; add phase D to adjust the pH to 6-6.5, stir and cool to 40°C; add phase E and stir thoroughly until the material is uniform, and cool to 37°C.

[0087] Application Example 3: Preparation method of emulsion containing Artemisia annua volatile oil

[0088] This application example provides a method for preparing an emulsion containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the emulsion is shown in Table 7.

[0089] Table 7 Formula of Artemisia annua volatile oil emulsion

[0090]

[0091]

[0092] The preparation process of the emulsion is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization, and homogenize with appropriate homogenization force for 3-10 minutes; stir and cool to 50°C, add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, and cool to 37°C before discharging.

[0093] Application Example 4: Preparation method of essence containing Artemisia annua volatile oil

[0094] This application example provides a method for preparing an essence containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the essence is shown in Table 8.

[0095] Table 8 Formula containing Artemisia annua volatile oil essence

[0096]

[0097] The preparation process of the essence is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B to phase A under homogenization; add phase C, homogenize with appropriate homogenization force for 3-10 minutes, stir and cool to 50°C; add phase D to adjust the pH to 6-6.5, stir and cool to 40°C; add phase E and stir thoroughly until the material is uniform, cool to 37°C and then discharge.

[0098] Application Example 5: Preparation method of toner containing Artemisia annua volatile oil

[0099] This application example provides a method for preparing a toner containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the toner is shown in Table 9.

[0100] Table 9: Formula of toner containing Artemisia annua volatile oil

[0101]

[0102] The preparation process of toner water is as follows: mix phase A, heat, stir until dissolved and transparent, add phase B after cooling to adjust the pH to 6-6.5; add phase C, stir and mix until the material is uniform, and filter out the material.

[0103] Application Example 6: Preparation method of gel containing Artemisia annua volatile oil

[0104] This application example provides a method for preparing a jelly containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the jelly is shown in Table 10.

[0105] Table 10 Formula containing Artemisia annua volatile oil gel

[0106]

[0107] The preparation process of the gel is as follows: heat phase A and phase B to 80°C respectively, slowly add phase B into phase A under homogenization; add phase C, homogenize with appropriate homogenization force for 3-10 minutes, stir and cool to 50°C; add phase C to adjust the pH to 6-6.5, stir and cool to 40°C; add phase D and stir thoroughly until the material is uniform, cool to 37°C and then discharge.

[0108] Application Example 7: Preparation method of facial mask containing Artemisia annua volatile oil

[0109] This application example provides a method for preparing a facial mask containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial mask is shown in Table 11.

[0110] Table 11 Formula of facial mask containing Artemisia annua volatile oil

[0111]

[0112] The preparation process of the facial mask is as follows: mix phase A, heat, stir until dissolved and transparent, add phase B after cooling to adjust the pH to 6-6.5; add phase C, stir and mix until the material is uniform and then discharge.

[0113] Application Example 8: Preparation method of facial cleanser containing Artemisia annua volatile oil

[0114] This application example provides a method for preparing a facial cleanser containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the facial cleanser is shown in Table 12.

[0115] Table 12 Formula of facial cleanser containing Artemisia annua volatile oil

[0116]

[0117]

[0118] The preparation process of the facial cleanser is as follows: mix phase A, heat to 75°C, and stir until dissolved and transparent; add phase B to adjust the pH to 6.5-7, stir and cool to 50°C; add phase C, start controlled cooling and crystallization, and stop stirring and discharge after the temperature drops to 37°C.

[0119] Application Example 9: Preparation method of facial cleansing foam containing Artemisia annua volatile oil

[0120] This application example provides a method for preparing a cleansing foam containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the cleansing foam is shown in Table 13.

[0121] Table 13 Formula of cleansing foam containing Artemisia annua volatile oil

[0122]

[0123] The preparation process of the cleansing foam is as follows: heat and mix phase A until it becomes transparent, and after cooling, add phase B to adjust the pH to 6.5-7; add phase C, stir evenly, and then discharge.

[0124] Application Example 10: Preparation method of spray containing Artemisia annua volatile oil

[0125] This application example provides a method for preparing a spray containing the above-mentioned Artemisia annua volatile oil, wherein the formula of the spray is shown in Table 14.

[0126] Table 14 contains the formula of Artemisia annua volatile oil spray

[0127]

[0128] The preparation process of the spray is as follows: Phase A is heated, mixed and stirred until transparent, after cooling, Phase B is added, stirred and mixed until the material body is uniform, and the material is filtered out.

[0129] Example 3: Antioxidant function of Artemisia annua volatile oil composition

[0130] In this example, the antioxidant effect of the sample to be tested is evaluated by detecting the change in the content of reactive oxygen species (ROS). The parameters of each group are shown in Table 15, the specific operation method is as described in Example 1, and the statistical results of ROS fluorescence density are shown in Table 16.

[0131] Table 15 Groups and parameter settings for the test of the antioxidant function of Artemisia annua volatile oil

[0132]

[0133] Table 16 ROS fluorescence density statistical results

[0134]

[0135]

[0136] Note: The integrated optical density (IOD) reflects the content of ROS. When the t-test method is used for statistical analysis, compared with the BC group, the significance is represented by #, that is, P-value < 0.05 is represented by #, and P-value < 0.01 is represented by ##; compared with the NC group, the significance is represented by *, that is, P-value < 0.01 is represented by 0.01.

[0137] P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **

[0138] As shown in Table 16, the inhibitory effect of Artemisia annua volatile oil at a concentration of only 0.16% on the generation of ROS is better than that of 5% acetyl hexapeptide-8 (solution) and 2% palmitoyl tripeptide-5 (solution), which further illustrates the outstanding antioxidant property of Artemisia annua volatile oil; the combination of Artemisia annua volatile oil with acetyl hexapeptide-8 or palmitoyl tripeptide-5 can further reduce the level of ROS, indicating that the combination of Artemisia annua volatile oil and acetyl hexapeptide-8 or palmitoyl tripeptide-5 has a synergistic effect, and the synergistic effect of the combination of Artemisia annua volatile oil and acetyl hexapeptide-8 is better than that of the combination of Artemisia annua volatile oil and palmitoyl tripeptide-5; and when the composition contains Artemisia annua volatile oil, acetyl hexapeptide-8 and palmitoyl tripeptide-5 at the same time, the inhibitory effect is the best, indicating that the combination of these three chemical substances has a synergistic effect. In summary, it is preferred to add a combination of Artemisia annua volatile oil, acetyl hexapeptide-8 and palmitoyl tripeptide-5 to improve the antioxidant properties of the product; when the composition contains only two chemical components, the combination of Artemisia annua volatile oil and acetyl hexapeptide-8 is preferred.

[0139] Example 4: Anti-wrinkle effect of Artemisia annua volatile oil composition

[0140] In this embodiment, UVB is used to irradiate the 3D epidermal skin model. The anti-wrinkle efficacy of the samples was evaluated by detecting the changes in the content of matrix metalloproteinase 1 (MMP-1). The parameters of each group are shown in Table 17. The specific operation steps are as shown in Example 2. The results are shown in Table 18.

[0141] Table 17 Groups and parameter settings for the anti-wrinkle efficacy test of Artemisia annua volatile oil

[0142]

[0143]

[0144] Table 18 Detection results of MMP-1 content

[0145]

[0146] Note: When using t-test for statistical analysis, compared with the BC group, the significance is indicated by #, that is, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, that is, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by

[0147] **

[0148] As shown in Table 18, the inhibitory effect of Artemisia annua volatile oil at a concentration of only 0.16% on the MMP-1 content is better than that of 5% acetyl hexapeptide-8 (solution) and 2% palmitoyl tripeptide-5 (solution), further illustrating the outstanding anti-wrinkle effect of Artemisia annua volatile oil; the combination of Artemisia annua volatile oil with acetyl hexapeptide-8 or palmitoyl tripeptide-5 can further reduce the content of MMP-1, indicating that the combination of Artemisia annua volatile oil and acetyl hexapeptide-8 or palmitoyl tripeptide-5 has a synergistic effect, and the synergistic effect of the combination of Artemisia annua volatile oil and palmitoyl tripeptide-5 is better than that of the combination of Artemisia annua volatile oil and acetyl hexapeptide-8; and when the composition contains Artemisia annua volatile oil, acetyl hexapeptide-8 and palmitoyl tripeptide-5 at the same time, the inhibitory effect is the best, indicating that the combination of these three chemical substances has a synergistic effect. In summary, it is preferred that the combination of Artemisia annua volatile oil, acetyl hexapeptide-8 and palmitoyl tripeptide-5 is added to improve the anti-wrinkle effect of the product; when the composition contains only two chemical components, the combination of Artemisia annua volatile oil and palmitoyl tripeptide-5 is preferred.

[0149] The present invention also evaluated the antioxidant and anti-wrinkle effects of Artemisia annua volatile oil at concentrations of 0.00001-20% (v / v), and the effects were consistent with those described in Examples 1-4.

[0150] It should be understood that other experimental models can be used to evaluate the antioxidant and / or anti-wrinkle functions of the chemical substances mentioned in the present invention, such as cell models or in vitro skin models and other experimental systems; other positive controls can also be used according to the purpose and needs of the experiment; the chemical substances involved in the present invention can be combined with different formula systems and can be used in many types of products such as facial creams, eye creams, lotions, essences, toners, gels, facial masks, facial cleansers, facial cleansing honeys, facial cleansing foams, sunscreens, shower gels, sprays, etc.

[0151] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention; therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is limited by the attached claims rather than the above description. Therefore, it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the present invention, and any figure marks in the claims should not be regarded as limiting the claims involved.

[0152] 1.Kan, T., et al., Artemisia Naphta: A novel oil extract for sensitive and acne prone skin. Annals of Dermatological Research, 2021.5(1):p.022-029.

[0153] 2.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on atopic dermatitis mouse model based on Th1 / Th2 balance. Medical Aesthetics, 2022(10): p.1-5.

[0154] 3.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on skin barrier damage model mice and skin microecology. Medical Aesthetics, 2022.31(16):p.1-7.

[0155] 4.Huang Hu, et al., Intervention effect of artemisia annua oil on psoriasis mouse model. Chinese Journal of Integrated Traditional and Western Medicine Dermatology and Venereology, 2024.23(01):p.1-5.

[0156] 5.Huang Hu, et al., Study on the intervention effect of artemisia annua oil on mouse acne model and skin microecology. Chinese Journal of Leprosy and Skin Diseases, 2024.40(09):p.624-629.

[0157] 6.Huang Hu, et al., Study on the efficacy and mechanism of artemisia annua oil on acne in mice. Journal of Dermatology and Venereology, 2024.31(06):p.374-380.

[0158] 7.Huang Hu, et al., Effects of artemisia annua oil on guinea pig seborrheic dermatitis model. Chinese Journal of Leprosy and Skin Diseases, 2024.40(08):p.555-559.

[0159] 8. Huang, H., et al., Novel anti-inflammatory Artemisia Naphta oilextract efficacious in in vivo mouse models of atopic dermatitis and psoriasis. Research Square, 2023.

[0160] 9. Qin Ziyan, et al., The development history of Kunming Artemisia annua raw materials and new applications in sensitive skin care. Chinese Cosmetics, 2022(10): p.122-126.

[0161] 10. Wang Sha, et al., Effect and safety of skin care products containing Artemisia annua essential oil in people with sensitive skin. Journal of Dermatology and Venereology, 2024.31(02):p.67-76.

Claims

1. Use of Artemisia annua volatile oil in preparing a preparation for improving the antioxidant effect of the product.

2. The use according to claim 1, characterized in that The usage concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

3. The use according to claim 2, characterized in that The antioxidant is that the Artemisia annua volatile oil inhibits the generation of active oxygen.

4. The use according to claim 3, characterized in that The products include but are not limited to facial cream, eye cream, lotion, essence, toner, gel, facial mask, facial cleanser, facial honey, facial foam, sunscreen, shower gel, spray, etc.

5. Use of Artemisia annua volatile oil in preparing a preparation for improving the anti-wrinkle effect of a product.

6. The use according to claim 5, characterized in that The usage concentration of the Artemisia annua volatile oil is 0.00001-20% (v / v).

7. The use according to claim 6, characterized in that The anti-wrinkle effect is that the Artemisia annua volatile oil downregulates the content of matrix metalloproteinase 1.

8. Use of Artemisia annua volatile oil to improve the antioxidant effect of products by inhibiting the generation of active oxygen.

9. Use of Artemisia annua volatile oil to improve the anti-wrinkle effect of products by down-regulating the content of matrix metalloproteinase 1.

10. Use of a composition for preparing an agent for improving the antioxidant and / or anti-wrinkle efficacy of a product, characterized in that: The composition comprises at least one of Artemisia annua volatile oil, acetyl hexapeptide-8 and palmitoyl tripeptide-5.

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