Composition for enhancing immunity and application of composition in medicine for improving fatty liver

Abstract

The invention provides a composition for enhancing immunity and application of the composition in medicines for improving fatty liver. The composition for enhancing immunity comprises the following components in parts by weight: 5-10 parts of ginseng, 10-15 parts of kudzuvine root, 10-15 parts of medlar, 15-20 parts of hawthorn, 10-20 parts of rhizoma polygonati, 5-10 parts of pine pollen, 10-20 parts of Chinese yam, 10-20 parts of poria cocos and 3-10 parts of vine tea. The invention further discloses a preparation method of the composition for enhancing the immunity and application of the composition for improving and / or preventing and / or treating the fatty liver. The composition for enhancing the immunity is a medicinal and edible pure natural antioxidant composition, has no toxic or side effect, is easy to absorb, can be used as a dietary supplement or a health food raw material, and has a good liver protection effect.

Description

Technical Field

[0001] The invention relates to traditional Chinese medicine technology, and in particular to a composition for enhancing immunity and application thereof in a drug for improving fatty liver. Background Art

[0002] Food and medicine ingredients refer to active ingredients that can be used as food for daily consumption and as medicines for the prevention and treatment of diseases. They have traditional consumption habits and the function of preventing and treating diseases. As early as the Neijing era, people already had the concept of food and medicine. From "five grains for nourishment, five fruits for assistance, five animals for benefit, five vegetables for filling, take them with flavors and tastes to replenish essence and qi." The Yellow Emperor's Neijing initially realized the nutritional supplementary effect of food on the human body, and the medical books such as Shennong's Herbal Classic, Compendium of Materia Medica, Qianjin Yaofang and Taiping Shenghui Fang included a large number of medicinal and edible plants, and clearly recorded the efficacy and main indications of Chinese medicines for both medicinal and edible purposes. People continue to develop the theoretical ideas of food nourishment and food therapy in daily practice. To date, Chinese medicines that are both edible and medicinal have not only been used as food for health care, but also have a very broad application prospect in disease prevention and treatment. They are used to reduce blood lipids, fight fatigue, fight alcoholic liver damage, treat osteoporosis, protect the retina, prevent and treat Alzheimer's disease and cardiovascular diseases, as well as fight cancer and prevent and treat epidemics. In the process of the occurrence and development of chronic liver disease (ALD), Chinese medicines that are both edible and medicinal can play a certain preventive and therapeutic effect. Because of their dual-purpose nature, they have high patient compliance and have unique advantages over other drugs. These ingredients usually have multiple pharmacological activities, and daily consumption is beneficial to people's health. Medicinal and edible ingredients have the advantages of high safety and wide sources, so they have the potential to be developed into new drugs.

[0003] There are many Chinese medicines with the function of sobering up and protecting the liver. They can not only play a role in clinical practice, but also prevent the formation of ALD and slow down the course of the disease in daily life. They have broad market prospects, so many studies on Chinese medicine compound prescriptions against ALD have been derived. Chinese medicines with the function of sobering up and protecting the liver have the function of sobering up and protecting the liver. The combination of Chinese medicine and food with the function of sobering up and protecting the liver can prevent liver damage.

[0004] Therefore, it is important to develop a medicine-food combination that can enhance immunity and protect the liver. Summary of the invention

[0005] The purpose of the present invention is to propose an immunity enhancing composition in view of the above problems. The composition is a pure natural and antioxidant composition that is both medicinal and edible. The composition has no toxic side effects and is easily absorbed. It can be used as a dietary supplement or a health food raw material and has good liver protection effect.

[0006] It should be noted that, in the present invention, unless otherwise specified, the specific meaning of "including" in relation to composition limitations and descriptions includes both open-ended "including", "comprising", etc. and similar meanings, as well as closed-ended "composed of", "composed of", etc. and similar meanings.

[0007] To achieve the above object, the technical solution adopted by the present invention is: a composition for enhancing immunity, comprising the following components in weight proportions:

[0008] 5-10 parts of ginseng, 10-15 parts of kudzu root, 10-15 parts of wolfberry, 15-20 parts of hawthorn, 10-20 parts of polygonatum, 5-10 parts of pine pollen, 10-20 parts of yam, 10-20 parts of poria and 3-10 parts of rattan tea.

[0009] Furthermore, the preferred composition for enhancing immunity comprises the following components in proportion by weight:

[0010] 7-9 parts of ginseng, 10-12 parts of kudzu root, 12-14 parts of wolfberry, 15-18 parts of hawthorn, 10-15 parts of polygonatum, 7-9 parts of pine pollen, 12-15 parts of yam, 15-18 parts of poria and 5-8 parts of rattan tea.

[0011] Furthermore, the most preferred composition for enhancing immunity comprises the following components in weight proportion:

[0012] 8 parts of ginseng, 10 parts of kudzu root, 12 parts of wolfberry, 15 parts of hawthorn, 10 parts of polygonatum, 8 parts of pine pollen, 12 parts of yam, 15 parts of tuckahoe and 8 parts of rattan tea.

[0013] Furthermore, the composition for enhancing immunity is prepared in any one of a liquid dosage form, a solid dosage form or a semisolid dosage form (decoction).

[0014] Furthermore, the composition for enhancing immunity also includes a carrier or additive acceptable in pharmaceutical or food production.

[0015] Furthermore, the pharmaceutically or food production acceptable carrier or additive includes: any one or more of flavoring agents, fillers, lubricants, adhesives, cross-linking agents, pH adjusters, humectants, preservatives, antioxidants or solvents.

[0016] Another object of the present invention is to disclose a method for preparing a composition for enhancing immunity, comprising the following steps:

[0017] Step 1: clean the raw materials ginseng, kudzu root, wolfberry, hawthorn, polygonatum, pine pollen, yam, poria and rattan tea, chop them and mix them according to the weight ratio;

[0018] Step 2, put the mixture into a blender, and add water according to the solid-liquid ratio of 1:7-1:10, stir after adding, soak for 2-5 hours, extract at 45-50°C for 2-3 hours, and filter; the purpose of soaking is to allow the liquid and material to be fully mixed to reach a certain humidity, and the extraction is to extract the extract inside by temperature after soaking.

[0019] Step 3: vacuum concentrate the filtrate to a solid content of 20-50 wt.% to obtain a decoction.

[0020] Furthermore, the particle size of the raw material after shredding in step 1 is 200-300 meshes.

[0021] Furthermore, the concentration time in step 3 is 2-3 hours and the concentration temperature is 40-50°C

[0022] Furthermore, a pharmaceutically or food production acceptable carrier or additive is added to the decoction in step 3.

[0023] Another object of the present invention is to disclose the use of a composition for enhancing immunity in improving and / or preventing and / or treating fatty liver.

[0024] Further, once a day, the dosage is 0.2-0.3 g, preferably 0.25 g.

[0025] Compared with the prior art, the composition for enhancing immunity of the present invention and its application in the drug for improving fatty liver have the following advantages:

[0026] 1) Formula ingredients used in the present invention:

[0027] Ginseng extract: contains a variety of active ingredients, such as anthraquinones, flavonoids, phenolic glycosides, saponins, phenolic acids and volatile oils, which have antioxidant and anti-inflammatory properties and help protect the liver.

[0028] Pueraria extract: can significantly inhibit the oxidative damage of mitochondria in liver cells, prevent mitochondrial swelling and the decrease of ATPase activity, and has the function of effectively removing superoxide anion free radicals produced by mitochondria.

[0029] Lycium barbarum and hawthorn have the effects of clearing liver, detoxifying, protecting and benefiting liver. Polygonatum sibiricum extract contains polysaccharides, steroidal saponins, anthraquinone compounds, alkaloids, cardiac glycosides, lignans, vitamins and a variety of amino acids useful to human body, which have the effects of widening stomach and replenishing qi, benefiting kidney and protecting liver, nourishing yin and moistening lung, promoting body fluid and tonifying spleen. Polygonatum sibiricum extract can improve liver poisoning, reduce liver edema, significantly reduce liver damage, prevent liver cell damage and destruction, thus achieving the purpose of protecting liver cells.

[0030] Pine pollen extract contains pollen polysaccharides, proteins, cellulose, unsaturated fatty acids and trace elements. It also contains bioactive ingredients such as polyphenol compounds and phytosterols, which have a preventive effect on liver damage caused by a high-fat diet.

[0031] Yam and Poria cocos have the effects of strengthening the spleen and lungs, consolidating the kidneys and improving essence, nourishing the liver and kidneys, clearing away heat and moistening dryness.

[0032] Tengcha extract has obvious liver protection and enzyme lowering effects, and has the effect of resisting chronic liver fibrosis.

[0033] The present invention uses the above-mentioned Chinese medicinal materials that are both medicinal and edible in combination, so that the active ingredients in each medicinal material produce a synergistic effect and mutually enhance the liver protection and liver care effects, thereby effectively inhibiting and preventing liver tissue damage.

[0034] 2) The present invention determines that the composition has a liver-protecting effect based on the theory of traditional Chinese medicine. The antioxidant capacity of the composition preparations is evaluated respectively, and it has a good antioxidant effect and a good protective effect on the liver.

[0035] 3) The present invention adopts traditional Chinese medicine for direct preparation, has a simple manufacturing method, low cost, and the obtained composition is in a paste form and is easy to use.

[0036] In summary, the present invention adopts different liver-protecting plants that are both medicinal and edible, and extracts effective active ingredients therein, so that they can play a liver-protecting role in multiple aspects and multiple targets, and achieve the effects of anti-oxidation and lipid-lowering. DETAILED DESCRIPTION

[0037] The present invention is further described below with reference to the examples. The description of the technical features described below is based on representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples. It should be noted that:

[0038] Unless otherwise stated, the units used in this specification are all international standard units, and the numerical values ​​and numerical ranges appearing in the present invention should be understood to include the inevitable systematic errors in industrial production.

[0039] In this specification, the numerical range expressed using "a numerical value A to a numerical value B" means a range including the endpoints numerical values ​​A and B.

[0040] In the present specification, a numerical range expressed using "above" or "below" means a numerical range including the number.

[0041] In this specification, the word "may" means both performing a certain process and not performing a certain process.

[0042] In this specification, the use of "optional" or "optional" indicates that certain substances, components, execution steps, application conditions and other factors are used or not used.

[0043] In this specification, when "normal temperature" or "room temperature" is used, the temperature may be 15-25°C.

[0044] In this manual, the reagents or instruments used without indicating the manufacturer are all conventional products that can be obtained through commercial purchase.

[0045] Unless otherwise specified, % in the present invention refers to mass percentage and ratios refer to mass ratios.

[0046] Example 1

[0047] This embodiment discloses a medicinal and edible composition for enhancing immunity, which is made from the following raw materials in parts by weight: 8 parts of ginseng, 10 parts of kudzu root, 10 parts of wolfberry, 15 parts of hawthorn, 10 parts of polygonatum, 8 parts of pine pollen, 10 parts of yam, 10 parts of poria and 5 parts of rattan tea.

[0048] The medicine-food homologous composition for enhancing immunity is prepared by the following method:

[0049] Step 1: Clean the ingredients of ginseng, kudzu root, wolfberry, hawthorn, polygonatum, pine pollen, yam, poria and rattan tea, mix them according to the weight ratio, and chop them before mixing.

[0050] Step 2, put the mixture into a blender, and add the aqueous solution according to the solid-liquid ratio of 1:10, stir after adding, soak for 5 hours, extract at 45°C for 2 hours, and filter; the purpose of soaking is to allow the liquid and material to be fully mixed to reach a certain humidity, and the extraction is to extract the extract inside by temperature after soaking.

[0051] Step 3: vacuum concentrate the filtrate to a solid content of 30%, the concentration time is 2 hours, and the concentration temperature is 40° C. After concentration, conventional auxiliary materials are added to prepare a decoction paste.

[0052] Example 2

[0053] This embodiment discloses a medicinal and edible composition for enhancing immunity, which is made from the following raw materials in parts by weight: 10 parts of ginseng, 15 parts of kudzu root, 15 parts of wolfberry, 15 parts of hawthorn, 15 parts of polygonatum, 10 parts of pine pollen, 10 parts of yam, 10 parts of poria and 8 parts of rattan tea.

[0054] The medicine-food homologous composition for enhancing immunity is prepared by the following method:

[0055] Step 1: Clean the ingredients of ginseng, kudzu root, wolfberry, hawthorn, polygonatum, pine pollen, yam, poria and rattan tea, mix them according to the weight ratio, and chop them before mixing.

[0056] Step 2, put the mixture into a blender, and add the aqueous solution according to the solid-liquid ratio of 1:7, stir after adding, soak for 4 hours, extract at 50°C for 3 hours, and filter; the purpose of soaking is to allow the liquid and material to be fully mixed to reach a certain humidity, and the extraction is to extract the extract inside by temperature after soaking.

[0057] Step 3: vacuum concentrate the filtrate to a solid content of 30%, the concentration time is 3 hours, and the concentration temperature is 45° C. After concentration, conventional auxiliary materials are added to prepare a decoction paste.

[0058] Example 3

[0059] This embodiment discloses a medicinal and edible composition for enhancing immunity, which is made from the following raw materials in parts by weight: 10 parts of ginseng, 10 parts of kudzu root, 12 parts of wolfberry, 18 parts of hawthorn, 10 parts of polygonatum, 10 parts of pine pollen, 20 parts of yam, 10 parts of poria and 10 parts of rattan tea.

[0060] The medicine-food homologous composition for enhancing immunity is prepared by the following method:

[0061] Step 1: Clean the ingredients of ginseng, kudzu root, wolfberry, hawthorn, polygonatum, pine pollen, yam, poria and rattan tea, mix them according to the weight ratio, and chop them before mixing.

[0062] Step 2, put the mixture into a blender, and add aqueous solution according to the solid-liquid ratio of 1:8, stir after adding, soak for 2.5 hours, extract at 45°C for 3 hours, and filter; the purpose of soaking is to allow the liquid and material to be fully mixed to reach a certain humidity, and the extraction is to extract the extract inside by temperature after soaking.

[0063] Step 3: vacuum concentrate the filtrate to a solid content of 25%, the concentration time is 2 hours, and the concentration temperature is 40° C. After concentration, conventional auxiliary materials are added to prepare a decoction paste.

[0064] Brew with warm water in a 1:1 ratio and you can drink.

[0065] Control group

[0066] The control group used Vc in the manner and frequency specified in the product instructions.

[0067] In order to further illustrate the efficacy of the food-medicine composition for enhancing immunity of the present invention, the following test was conducted for evaluation:

[0068] Free radical scavenging ability test of the composition

[0069] According to Examples 1, 2, and 3, medicinal and edible compositions 1, 2, and 3 (referred to as compositions 1, 2, and 3) for enhancing immunity were prepared respectively. Vc was used for comparative analysis in the control group, and the antioxidant capacity was evaluated by the DPPH method.

[0070] Test method: Take compositions 1, 2, 3 and control group Vc respectively, prepare different concentrations (10, 50, 100, 200, 400 μg / mL) of test solution, set positive control VC (8, 12, 16, 20, 24 μg / mL), take 2 mL of sample and add 2 mL of 0.1 mmol / LDPPH free radical solution, shake well, protect from light for 30 minutes, and measure the absorbance Ai at a wavelength of 517 nm; replace the DPPH free radical solution with an equal volume of anhydrous ethanol, and measure the absorbance Aj; replace the sample with an equal volume of anhydrous ethanol, and measure the absorbance Ae. The calculation formula of DPPH free radical scavenging rate is: K = [1-(Ai-Aj) / Ae] × 100%. The obtained data is used to calculate the IC50 value by software Graphpad Prism 6.0 to evaluate the antioxidant effect of the sample.

[0071] Table 1 Test results of free radical scavenging ability of the composition

[0072]

[0073] The test results are shown in Table 1. Compositions 1, 2, and 3 all showed good DPPH free radical scavenging ability, among which composition 3 had the best DPPH free radical scavenging effect, with an IC50 value of 50.43±5.31 μg / mL, indicating that the composition has a good antioxidant effect.

[0074] Effect of the composition on liver function

[0075] The medication was taken once a day, with a dose of 0.25 g. Blood was collected before and after the treatment, serum was separated, and liver function (ALT, AST, GGT) was tested using an automatic blood biochemical analyzer. The test results are shown in Table 2. It can be seen that compositions 1, 2, and 3 all showed good liver protection effects.

[0076] Table 2 Effect of the composition on liver function

[0077]

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition for enhancing immunity, characterized in that: The composition includes the following components in weight ratio: 5-10 parts of ginseng, 10-15 parts of kudzu root, 10-15 parts of wolfberry, 15-20 parts of hawthorn, 10-20 parts of polygonatum, 5-10 parts of pine pollen, 10-20 parts of yam, 10-20 parts of poria and 3-10 parts of rattan tea.

2. The composition for enhancing immunity according to claim 1, characterized in that: The composition includes the following components in weight ratio: 7-9 parts of ginseng, 10-12 parts of kudzu root, 12-14 parts of wolfberry, 15-18 parts of hawthorn, 10-15 parts of polygonatum, 7-9 parts of pine pollen, 12-15 parts of yam, 15-18 parts of poria and 5-8 parts of rattan tea.

3. The composition for enhancing immunity according to claim 1, characterized in that: The composition for enhancing immunity is prepared in any one of liquid dosage form, solid dosage form or semisolid dosage form.

4. The composition for enhancing immunity according to claim 1, characterized in that: The composition for enhancing immunity also includes carriers or additives acceptable in pharmaceutical or food production.

5. The composition for enhancing immunity according to claim 4, characterized in that: The pharmaceutically or food-acceptable carrier or additive comprises any one or more of a flavoring agent, a filler, a lubricant, a binder, a cross-linking agent, a pH regulator, a moisturizer, a preservative, an antioxidant or a solvent.

6. A method for preparing the composition for enhancing immunity according to any one of claims 1 to 5, characterized in that: The following steps are involved: Step 1: clean the raw materials ginseng, kudzu root, wolfberry, hawthorn, polygonatum, pine pollen, yam, poria and rattan tea, chop them and mix them according to the weight ratio; Step 2, putting the mixture into a blender, and adding water according to a solid-liquid ratio of 1:7-1:10, stirring after adding water, soaking for 2-5 hours, extracting at 45-50° C. for 2-3 hours, and filtering; Step 3: vacuum concentrate the filtrate to a solid content of 20-50 wt.% to obtain a decoction.

7. The method for preparing the composition for enhancing immunity according to claim 6, characterized in that: Step 1: The raw material is chopped into particles with a size of 200-300 mesh.

8. The method for preparing the composition for enhancing immunity according to claim 6, characterized in that: The concentration time in step 3 is 2-3 hours, and the concentration temperature is 40-50°C.

9. The method for preparing the composition for enhancing immunity according to claim 6, characterized in that: In step 3, a carrier or additive acceptable in pharmaceutical or food production is added to the decoction.

10. Use of the composition for enhancing immunity according to any one of claims 1 to 5 in improving and / or preventing and / or treating fatty liver.

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