Cell extraction and storage device for flow cytometry detection

Abstract

The invention discloses a cell extraction and storage device for flow cytometry, which relates to the field of cell storage and comprises a test tube guide device embedded in a workbench and positioned at the bottom of a liquid discharge end of a flow cytometry instrument, and a base box arranged on the workbench and positioned on one side of the test tube guide device, the self-contraction bearing frame is used for clamping a plurality of test tubes, and a bearing frame traction transposition device, a bearing frame transfer device and a bearing frame cryopreservation device are sequentially arranged on the base box. The invention relates to a cell storage device which is convenient for standardized collection of a sample group generated by cell separation of a flow cytometer.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of cell storage, and in particular to a cell extraction and storage device for flow cytometry detection. Background Art

[0002] Flow cytometer is a device for automatic analysis and sorting of cells. It can rapidly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out designated cell subpopulations according to a preselected parameter range.

[0003] When using a flow cytometer for cell separation, place the test tube containing the cells at the suction end of the flow cytometer, and place a marked sample tube at the corresponding position at the discharge end of the flow cytometer. One or more sample tubes for a cell separation work are a sample group. Storing each sample group separately will help standardize the experiment and facilitate subsequent work.

[0004] The existing cell extraction and storage devices are not convenient for standardized collection and storage of sample groups generated by flow cytometer cell separation, and are not convenient for reasonable adjustment of sample storage space according to the number of samples. Summary of the invention

[0005] Based on this, the object of the present invention is to provide a cell extraction and storage device for flow cytometry detection to solve the technical problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: A cell extraction and storage device for flow cytometer detection, comprising a test tube guide device embedded on a workbench and located at the bottom of a flow cytometer discharge end, a base box disposed on the workbench and located at one side of the test tube guide device, and a self-shrinking carrier for clamping a plurality of test tubes, wherein a carrier pulling and transposing device, a carrier transfer device, and a carrier freezing device are sequentially disposed on the base box; The carrier pulling and transposing device comprises a rotating component provided on the base box and a pulling component provided on the workbench, the pulling component being located on the side of the test tube guiding device away from the base box, the rotating component being used to move the self-shrinking carrier to the test tube guiding device and the rotating component of the carrier transfer device, the pulling component being used to stretch the self-shrinking carrier, and the test tube guiding device being used to guide the test tube containing the sample into the stretched self-shrinking carrier; The carrier freezing device comprises a plurality of storage compartments arranged in a linear array on the base box, an electromagnetic door arranged at the end of the storage compartment, a movable partition arranged in the storage compartment, and a refrigeration component embedded in the movable partition; The carrier rack transfer device is used to move the self-shrinking carrier rack into the carrier rack freezing device.

[0007] Preferably, the self-contracting carrier includes a first carrier plate, a second carrier plate slidably connected to the bottom of the first carrier plate, a third carrier plate slidably connected to the bottom of the second carrier plate, and a fourth carrier plate slidably connected to the bottom of the third carrier plate; A circular through hole is formed on the first supporting plate, a U-shaped through hole is formed on the second supporting plate, the third supporting plate and the fourth supporting plate, and an arc-shaped groove is formed on the outer wall of the first supporting plate, the second supporting plate and the third supporting plate; The first supporting plate is provided with a first vertical plate at one end and a second vertical plate at one side; the fourth supporting plate is provided with a third vertical plate at one end; the first vertical plate and the third vertical plate are both provided with U-shaped tubes on their outer walls, and an elastic rope is provided between the two U-shaped tubes. In this preferred embodiment, the self-shrinking supporting frame is used to collect and close one or more sample tubes of a single sample group, so as to improve the space utilization efficiency.

[0008] Preferably, the rotating component includes a first micro motor embedded in the base box, a rotating disk disposed at the execution end of the first micro motor and arranged horizontally, and a plurality of first electromagnetic blocks arranged in an annular array on the outer wall of the rotating disk. In this preferred embodiment, the rotating component facilitates the self-contracting carrier to be moved to the test tube guiding device and the carrier transfer device in sequence.

[0009] Preferably, the pulling component comprises a micro-drive cylinder disposed on the workbench, a pulling plate disposed at the execution end of the micro-drive cylinder, and a second electromagnetic block disposed at the end of the pulling plate. In this preferred embodiment, the pulling component facilitates the unfolding of the closed self-contracting carrier.

[0010] Preferably, the test tube guiding device comprises a positioning box embedded on the workbench, a linear array of micro-electric cylinders arranged in the positioning box and having an execution end that penetrates the positioning box and extends to the upper part of the positioning box, a suction cup arranged at the execution end of the micro-electric cylinder, and a negative pressure source tube having one end connected to the suction cup. In this preferred embodiment, the test tube guiding device facilitates guiding the test tube carrying the sample to the unfolded self-contracting carrier.

[0011] Preferably, the airbag sealing component is provided at one end of the movable partition away from the electromagnetic door, and the airbag sealing component includes a support plate provided on the outer wall of the movable partition, an annular airbag provided on the outer wall of the support plate, and a friction cloth provided on the outer wall of the movable partition and located between the annular airbag and the storage compartment. In this preferred embodiment, the gap between the movable partition and the inner wall of the storage compartment is blocked by the airbag sealing component to enhance the heat preservation effect.

[0012] Preferably, the refrigeration component includes a semiconductor refrigeration plate and a liquid nitrogen evaporator embedded in the movable partition, and a liquid inlet pipe and a liquid outlet pipe, one end of which passes through the outer wall of the storage compartment and is connected to the liquid nitrogen evaporator. In this preferred embodiment, the temperature in the storage compartment can be easily adjusted by the refrigeration component.

[0013] Preferably, the base box is provided with a power component for driving the movement of the movable partition, and the power component includes a rotating shaft connected to the upper surface of the base box through a plurality of bearing seats, a power motor provided on the base box and used to drive the rotating shaft to rotate, a plurality of electromagnetic rings provided on the outer wall of the rotating shaft, a winch sleeved on the outer wall of the electromagnetic ring, a connecting rope with one end connected to the winch and the other end passing through the storage compartment and connected to the outer wall of the movable partition, and a spring provided on the inner wall of the storage compartment and used to provide support force to the movable partition. In this preferred embodiment, the position of the movable partition can be easily adjusted by the power component.

[0014] Preferably, the carrier transfer device includes a first linear module disposed in the base box and having an execution end passing through the base box and extending to the upper part of the base box, an L-shaped plate horizontally disposed at the execution end of the first linear module, a micro telescopic cylinder disposed on the outer wall of the L-shaped plate and having an execution end passing through the L-shaped plate, an L-shaped electromagnetic block disposed at the execution end of the micro telescopic cylinder, and an opening and closing component disposed at the end of the L-shaped plate. In this preferred embodiment, the carrier transfer device facilitates the self-retracting carrier to be moved into the storage compartment.

[0015] Preferably, the opening and closing component includes an L-shaped frame arranged at the end of the L-shaped plate, a second micro motor arranged at the top of the L-shaped frame and having an execution end passing through the L-shaped frame, and an opening and closing electromagnetic block arranged at the execution end of the second micro motor. In this preferred embodiment, the electromagnetic door is opened or closed by the opening and closing component.

[0016] In summary, the present invention mainly has the following beneficial effects: The device of the present invention facilitates the standardized collection and storage of sample groups generated by flow cytometer cell separation, automates the entire collection and storage process, and facilitates reasonable adjustment of the sample storage space according to the number of samples; In the device of the present invention, a self-contracting carrier is used to collect and bring together one or more sample tubes of a single sample group, so as to improve the space utilization efficiency; The self-shrinking carrier is conveniently moved to the test tube guide device and the carrier transfer device in sequence by the rotating component, the closed self-shrinking carrier is conveniently unfolded by the pulling component, the test tube carrying the sample is conveniently guided to the unfolded self-shrinking carrier by the test tube guide device, and the self-shrinking carrier is conveniently moved into the carrier freezing device by the carrier transfer device. In the carrier freezing device, the electromagnetic door is opened or closed by the opening and closing components, the position of the movable partition is adjusted by the power components to facilitate the adjustment of the frozen storage space, the gap between the movable partition and the inner wall of the storage compartment is sealed by the airbag sealing components to enhance the insulation effect, and the temperature in the storage compartment is adjusted by the refrigeration components. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is an axonometric diagram of the overall structure of the present invention; Figure 2 A top view of the overall structure of the present invention; Figure 3 It is a cross-sectional view of the overall structure of the present invention; Figure 4 It is an axonometric view of the self-shrinking carrier structure of the present invention; Figure 5 This is an axonometric diagram of the structure of the carrier pulling and transposing device of the present invention; Figure 6 It is an axonometric view of the structure of the carrier transfer device of the present invention; Figure 7 It is an axonometric view of the overall structure of the carrier freezing device of the present invention; Figure 8 It is an exploded view of the overall structure of the carrier freezing device of the present invention; Fig. 9 It is a cross-sectional view of the structure of the carrier shelf freezing device of the present invention.

[0018] Description of the drawings: 10, test tube guiding device; 11, positioning box; 12, micro electric cylinder; 13, suction cup; 14, negative pressure source tube; 20, base box; 30, self-contracting carrier; 31, first carrier plate; 311, circular through hole; 312, arc-shaped groove; 313, first vertical plate; 314, second vertical plate; 32, second carrier plate; 321, U-shaped through hole; 33, third carrier plate; 34, fourth carrier plate; 341, third vertical plate; 35, U-shaped tube; 36, elastic rope; 40, carrier frame pulling and transposition device; 41, rotating component; 411, first micro motor; 412, rotating disk; 413, first electromagnetic block; 42, pulling component; 421, micro drive cylinder; 422, traction plate; 423, second electromagnetic block ; 50. Carrier transfer device; 51. First linear module; 52. L-shaped plate; 53. Micro telescopic cylinder; 54. L-shaped electromagnetic block; 55. Opening and closing component; 551. L-shaped frame; 552. Second micro motor; 553. Opening and closing electromagnetic block; 60. Carrier freezing device; 61. Storage compartment; 62. Electromagnetic door; 63. Moving partition; 64. Airbag sealing component; 641. Support plate; 642. Annular airbag; 643. Friction cloth; 65. Refrigeration component; 651. Semiconductor refrigeration plate; 652. Liquid nitrogen evaporator; 653. Liquid inlet pipe; 654. Liquid outlet pipe; 66. Power component; 661. Rotating shaft; 662. Power motor; 663. Electromagnetic ring; 664. Winch; 665. Connecting rope; 666. Spring. DETAILED DESCRIPTION

[0019] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0020] The following describes an embodiment of the present invention based on its overall structure. Example

[0021] Please refer to the attached Figure 1 , 2 As shown in Figures 3, 4, and 5, in a preferred embodiment of the present invention, a cell extraction and storage device for flow cytometry detection includes a test tube guide device 10 embedded on a workbench and located at the bottom of the discharge end of the flow cytometer, a base box 20 provided on the workbench and located at one side of the test tube guide device 10, and a self-shrinking carrier 30 for clamping a plurality of test tubes, wherein a carrier pulling and transposing device 40, a carrier transfer device 50, and a carrier freezing device 60 are sequentially provided on the base box 20; The carrier pulling and transposing device 40 includes a rotating component 41 disposed on the base box 20 and used to move the self-shrinking carrier 30 to the test tube guiding device 10 and the carrier transfer device 50 in sequence, and a pulling component 42 disposed on the workbench and located on the side of the test tube guiding device 10 away from the base box 20, the pulling component 42 is used to stretch the self-shrinking carrier 30, and the test tube guiding device 10 is used to guide the test tube containing the sample into the stretched self-shrinking carrier 30; the self-shrinking carrier 30 includes a first carrier plate 31, a second carrier plate 32 slidably connected to the bottom of the first carrier plate 31, a third carrier plate 33 slidably connected to the bottom of the second carrier plate 32, and a fourth carrier plate 34 slidably connected to the bottom of the third carrier plate 33; The first carrier plate 31 is provided with a circular through hole 311, the second carrier plate 32, the third carrier plate 33 and the fourth carrier plate 34 are provided with a U-shaped through hole 321, and the outer walls of the first carrier plate 31, the second carrier plate 32 and the third carrier plate 33 are provided with an arc groove 312; The first carrier plate 31 is provided with a first vertical plate 313 at one end and a second vertical plate 314 at one side. The fourth carrier plate 34 is provided with a third vertical plate 341 at one end. The outer walls of the first vertical plate 313 and the third vertical plate 341 are both provided with U-shaped tubes 35, and an elastic rope 36 is provided between the two U-shaped tubes 35. The rotating component 41 includes a first micro motor 411 embedded in the base box 20, a rotating disk 412 arranged at the execution end of the first micro motor 411 and arranged horizontally, and a plurality of first electromagnetic blocks 412 arranged in an annular array on the outer wall of the rotating disk 412. 13, the pulling component 42 includes a micro-driving cylinder 421 arranged on the workbench, a traction plate 422 arranged at the execution end of the micro-driving cylinder 421, and a second electromagnetic block 423 arranged at the end of the traction plate 422, the test tube guiding device 10 includes a positioning box 11 embedded in the workbench, a micro-electric cylinder 12 with a linear array arranged in the positioning box 11 and an execution end passing through the positioning box 11 and extending to the upper part of the positioning box 11, a suction cup 13 arranged at the execution end of the micro-electric cylinder 12, and a negative pressure source tube 14 with one end connected to the suction cup 13.

[0022] It should be noted that, in this embodiment, the staff can place a sample tube containing the mixed cell fluid to be sorted at the suction end of the flow cytometer, and place a cell collection tube at the corresponding position of the discharge end of the flow cytometer, and the test tube can be clamped and fixed by an electric sample test tube clamp; First, the sample tubes need to be collected and brought together. At this time, the self-shrinking carrier 30 can be manually placed at the loading position of the rotating component 41 so that the rotating component 41 can absorb the self-shrinking carrier 30, or a self-shrinking carrier 30 loading device can be set to move the self-shrinking carrier 30 one by one to the loading position of the rotating component 41. After the rotating component 41 magnetically absorbs the self-shrinking carrier 30 at the loading position, it drives the self-shrinking carrier 30 to the position of the pulling component 42 and the carrier transfer device 50. When the self-shrinking carrier 30 reaches the position of the pulling component 42, the pulling component 42 stretches and unfolds the self-shrinking carrier 30, the electric sample test tube clamp cancels the clamping, and the test tube guiding device 10 guides the test tube into the self-shrinking carrier 30, and then the self-shrinking carrier 30 automatically shrinks and closes to complete the collection and bringing together of the sample tubes. Secondly, the sample tube needs to be transferred. A sample tube capping mechanism can be provided on the workbench to cap the sample tube during the process of transferring the sample tube from the position of the pulling component 42 to the position of the carrier transfer device 50. When the sample tube reaches the carrier transfer device 50, the carrier transfer device 50 moves the sample tube into the carrier freezing device 60. Finally, the sample tube is stored. The carrier transfer device 50 moves the self-shrinking carrier 30 holding the sample tube into the carrier freezing device 60 for freezing. The temperature can be lowered by 20 minutes at 4 degrees Celsius, 30 minutes at minus 20 degrees Celsius, overnight at minus 80 degrees Celsius, and stored in liquid nitrogen, so as to maintain high-quality storage of cells. Furthermore, the circular through hole 311 and the U-shaped through hole 321 in the self-contracting carrier 30 can be used to place a test tube. When the test tube is located in the U-shaped through hole 321, the test tube is clamped between the U-shaped through hole 321 and the arc-shaped groove 312. When there is no test tube in any of the U-shaped through holes 321 of the second carrier plate 32, the third carrier plate 33 and the fourth carrier plate 34, the second carrier plate 32, the third carrier plate 33 or the fourth carrier plate 34 will move toward the first carrier plate 31 under the action of the elastic rope 36. Furthermore, when the rotating component 41 is working, the first electromagnetic block 413 can be energized to magnetically fix the self-contracting carrier 30 through the first vertical plate 313, and the execution end of the first micro motor 411 can drive the rotating disk 412 to rotate, so as to drive the self-contracting carrier 30 to rotate; Furthermore, when the pulling component 42 is working, the execution end of the micro-driving cylinder 421 drives the traction plate 422 to move, and the second electromagnetic block 423 at the end of the traction plate 422 can be connected to the fourth supporting plate 34 through the third vertical plate 341 after being energized. At this time, the execution end of the micro-driving cylinder 421 can drive the self-contracting supporting frame 30 to expand; Furthermore, when the test tube guiding device 10 is working, the negative pressure source tube 14 can be connected to the negative pressure system, and the executing end of the micro electric cylinder 12 drives the suction cup 13 to move until the suction cup 13 abuts against the bottom of the test tube, and the negative pressure system connected to the negative pressure source tube 14 is turned on, and the suction cup 13 adsorbs the bottom of the test tube. The executing end of the micro electric cylinder 12 drives the test tube to descend until the test tube is located in the circular through hole 311 or the U-shaped through hole 321, and the guidance is completed.

[0023] Please refer to the attached Figure 1 , 6 As shown, in another preferred embodiment of the present invention, the carrier transfer device 50 is used to move the self-contracting carrier 30 into the carrier freezing device 60, and the carrier transfer device 50 includes a first linear module 51 arranged in the base box 20 and the execution end passes through the base box 20 and extends to the upper part of the base box 20, an L-shaped plate 52 horizontally arranged at the execution end of the first linear module 51, a micro telescopic cylinder 53 arranged on the outer wall of the L-shaped plate 52 and the execution end passes through the L-shaped plate 52, an L-shaped electromagnetic block 54 arranged at the execution end of the micro telescopic cylinder 53, and an opening and closing component 55 arranged at the end of the L-shaped plate 52, the opening and closing component 55 includes an L-shaped frame 551 arranged at the end of the L-shaped plate 52, a second micro motor 552 arranged at the top of the L-shaped frame 551 and the execution end passes through the L-shaped frame 551, and an opening and closing electromagnetic block 553 arranged at the execution end of the second micro motor 552.

[0024] It should be noted that, in the present embodiment, when the carrier transfer device 50 is working, the execution end of the first linear module 51 drives the L-shaped plate 52 to move until the L-shaped electromagnetic block 54 magnetically attracts the second vertical plate 314 to complete the connection with the self-contracting carrier 30, and the execution end of the first linear module 51 drives the L-shaped plate 52 to move until the opening and closing component 55 moves to the position of the electromagnetic door 62 to be opened, and the opening and closing component 55 opens the electromagnetic door 62, and the first linear module 51 and the micro telescopic cylinder 53 cooperate to move the self-contracting carrier 30 into the storage compartment 61 and locate it on the two slide rails on the inner wall of the storage compartment 61, and finally the first linear module 51 drives the L-shaped plate 52 to move again until the opening and closing component 55 moves to the position of the electromagnetic door 62 to be closed, and the opening and closing component 55 closes the electromagnetic door 62; Furthermore, when the opening and closing component 55 is working, the actuator end of the second micro motor 552 drives the opening and closing electromagnetic block 553 to rotate. After the opening and closing electromagnetic block 553 passes through the electromagnetic suction electromagnetic door 62, it can drive the electromagnetic door 62 to rotate.

[0025] Please refer to the attached Figure 1 , 7As shown in Figures 8 and 9, in another preferred embodiment of the present invention, the carrier rack freezing device 60 includes a plurality of storage compartments 61 arranged in a linear array on the base box 20, an electromagnetic door 62 arranged at the end of the storage compartment 61, a movable partition 63 arranged in the storage compartment 61, an airbag sealing component 64 arranged at the end of the movable partition 63 away from the electromagnetic door 62, a refrigeration component 65 embedded in the movable partition 63, and a power component 66 arranged on the base box 20 and used to drive the movable partition 63 to move, the airbag sealing component 64 includes a support plate 641 arranged on the outer wall of the movable partition 63, an annular airbag 642 arranged on the outer wall of the support plate 641, and a friction cloth 643 arranged on the outer wall of the movable partition 63 and located between the annular airbag 642 and the storage compartment 61, the The refrigeration component 65 includes a semiconductor refrigeration plate 651 and a liquid nitrogen evaporator 652 embedded on the movable partition 63, as well as a liquid inlet pipe 653 and a liquid discharge pipe 654, one end of which passes through the outer wall of the storage cabin 61 and is connected to the liquid nitrogen evaporator 652. The power component 66 includes a rotating shaft 661 connected to the upper surface of the base box 20 through a plurality of bearing seats, a power motor 662 provided on the base box 20 and used to drive the rotating shaft 661 to rotate, a plurality of electromagnetic rings 663 provided on the outer wall of the rotating shaft 661, a capstan 664 sleeved on the outer wall of the electromagnetic ring 663, a connecting rope 665 with one end connected to the capstan 664 and the other end passing through the storage cabin 61 and connected to the outer wall of the movable partition 63, and a spring 666 provided on the inner wall of the storage cabin 61 and used to provide support force to the movable partition 63.

[0026] It should be noted that, in this embodiment, when the carrier freezing device 60 is working, the power component 66 adjusts the position of the movable partition 63 as needed, so as to adjust the space and save energy. After the movable partition 63 moves to the specified position, the airbag sealing component 64 blocks the gap between the movable partition 63 and the storage compartment 61 and fixes the movable partition 63 through friction. The refrigeration component 65 can adjust the temperature in the storage compartment 61 after the test tube enters the storage compartment 61. Furthermore, when the power component 66 is working, the spring 666 gives elastic support force to the movable partition 63, so that the movable partition 63 moves in the direction of the electromagnetic door 62, and the power motor 662 is turned on. The execution end of the power motor 662 drives the rotating shaft 661 to rotate through the gear, and the electromagnetic ring 663 at the corresponding position is turned on. The electromagnetic ring 663 magnetically attracts the inner wall of the capstan 664. At this time, the rotating shaft 661 can drive the capstan 664 to rotate, and the capstan 664 drives the movable partition 63 to move through the connecting rope 665; Furthermore, when the airbag sealing component 64 is working, the annular airbag 642 can be connected to the air source pipe, one end of which passes through the storage cabin 61 and is connected to the air source system. When the movable partition 63 reaches the specified position, the valve on the air source system is opened, and the air source system inputs pressurized gas into the annular airbag 642, and the annular airbag 642 expands to block the gap, and at the same time, pressure is applied to the friction cloth 643, so that the friction cloth 643 is in close contact with the inner wall of the storage cabin 61, so as to limit the movable partition 63 by friction. Furthermore, when the refrigeration component 65 is working, the liquid nitrogen evaporator 652 is connected to the refrigeration system through the liquid inlet pipe 653 and the liquid discharge pipe 654. The semiconductor refrigeration plate 651 and the liquid nitrogen evaporator 652 can both perform cooling work. The semiconductor refrigeration plate 651 can cool down after being powered on, which is suitable for maintaining the temperature requirements of four degrees Celsius and minus twenty degrees. The liquid nitrogen evaporator 652 is suitable for maintaining minus eighty degrees and the temperature required for continuous cell preservation.

[0027] The working principle of the present invention is: The electrical components in the present invention are all triggered to operate by the PLC controller; The staff can place the sample tube containing the mixed cell fluid to be sorted at the suction end of the flow cytometer, and place the cell collection tube at the corresponding position at the discharge end of the flow cytometer. The test tube can be clamped and fixed by the electric sample test tube clamp; First, the sample tubes need to be collected and brought together. At this time, the self-shrinking carrier 30 can be manually placed at the loading position of the rotating component 41 so that the rotating component 41 can absorb the self-shrinking carrier 30, or a self-shrinking carrier 30 loading device can be set to move the self-shrinking carrier 30 one by one to the loading position of the rotating component 41. After the rotating component 41 magnetically absorbs the self-shrinking carrier 30 at the loading position, it drives the self-shrinking carrier 30 to the position of the pulling component 42 and the carrier transfer device 50. When the self-shrinking carrier 30 reaches the position of the pulling component 42, the pulling component 42 stretches and unfolds the self-shrinking carrier 30, the electric sample test tube clamp cancels the clamping, and the test tube guiding device 10 guides the test tube into the self-shrinking carrier 30, and then the self-shrinking carrier 30 automatically shrinks and closes to complete the collection and bringing together of the sample tubes. Secondly, the sample tube needs to be transferred. A sample tube capping mechanism can be provided on the workbench to cap the sample tube during the process of transferring the sample tube from the position of the pulling component 42 to the position of the carrier transfer device 50. When the sample tube reaches the carrier transfer device 50, the carrier transfer device 50 moves the sample tube into the carrier freezing device 60. Finally, the sample tube is stored. The carrier transfer device 50 moves the self-shrinking carrier 30 holding the sample tube into the carrier freezing device 60 for freezing. The temperature can be lowered by 20 minutes at 4 degrees Celsius, 30 minutes at minus 20 degrees Celsius, overnight at minus 80 degrees Celsius, and stored in liquid nitrogen, so as to maintain high-quality storage of cells. The circular through hole 311 and the U-shaped through hole 321 in the self-contracting carrier 30 can be used to place a test tube. When the test tube is located in the U-shaped through hole 321, the test tube is clamped between the U-shaped through hole 321 and the arc-shaped groove 312. When there is no test tube in any of the U-shaped through holes 321 of the second carrier plate 32, the third carrier plate 33 and the fourth carrier plate 34, the second carrier plate 32, the third carrier plate 33 or the fourth carrier plate 34 will move toward the first carrier plate 31 under the action of the elastic rope 36. When the rotating component 41 is working, the first electromagnetic block 413 can be energized to magnetically fix the self-contracting carrier 30 through the first vertical plate 313, and the execution end of the first micro motor 411 can drive the rotating disk 412 to rotate, thereby driving the self-contracting carrier 30 to rotate; When the pulling component 42 is working, the execution end of the micro-driving cylinder 421 drives the traction plate 422 to move, and the second electromagnetic block 423 at the end of the traction plate 422 is energized and can be magnetically connected to the fourth supporting plate 34 through the third vertical plate 341. At this time, the execution end of the micro-driving cylinder 421 can drive the self-contracting supporting frame 30 to expand; When the test tube guiding device 10 is working, the negative pressure source tube 14 can be connected to the negative pressure system, and the execution end of the micro electric cylinder 12 drives the suction cup 13 to move until the suction cup 13 abuts against the bottom of the test tube, and the negative pressure system connected to the negative pressure source tube 14 is turned on, the suction cup 13 adsorbs the bottom of the test tube, and the execution end of the micro electric cylinder 12 drives the test tube to descend until the test tube is located in the circular through hole 311 or the U-shaped through hole 321, and the guiding is completed; When the carrier transfer device 50 is working, the execution end of the first linear module 51 drives the L-shaped plate 52 to move until the L-shaped electromagnetic block 54 magnetically attracts the second vertical plate 314 to complete the connection with the self-contracting carrier 30. The execution end of the first linear module 51 drives the L-shaped plate 52 to move until the opening and closing component 55 moves to the position of the electromagnetic door 62 to be opened, and the opening and closing component 55 opens the electromagnetic door 62. The first linear module 51 and the micro telescopic cylinder 53 cooperate to move the self-contracting carrier 30 into the storage compartment 61 and locate it on the two slide rails on the inner wall of the storage compartment 61. Finally, the first linear module 51 drives the L-shaped plate 52 to move again until the opening and closing component 55 moves to the position of the electromagnetic door 62 to be closed, and the opening and closing component 55 closes the electromagnetic door 62. When the opening and closing component 55 is working, the actuator end of the second micro motor 552 drives the opening and closing electromagnetic block 553 to rotate. After the opening and closing electromagnetic block 553 passes through the electromagnetic suction electromagnetic door 62, it can drive the electromagnetic door 62 to rotate. When the carrier freezing device 60 is working, the power component 66 adjusts the position of the movable partition 63 as needed to adjust the space for energy saving. After the movable partition 63 moves to the specified position, the airbag sealing component 64 blocks the gap between the movable partition 63 and the storage compartment 61 and fixes the movable partition 63 by friction. The refrigeration component 65 can adjust the temperature in the storage compartment 61 after the test tube enters the storage compartment 61. When the power component 66 is working, the spring 666 gives elastic support force to the movable partition 63, so that the movable partition 63 moves toward the direction of the electromagnetic door 62, and the power motor 662 is turned on. The execution end of the power motor 662 drives the rotating shaft 661 to rotate through the gear, and the electromagnetic ring 663 at the corresponding position is turned on. The electromagnetic ring 663 magnetically attracts the inner wall of the capstan 664. At this time, the rotating shaft 661 can drive the capstan 664 to rotate, and the capstan 664 drives the movable partition 63 to move through the connecting rope 665; When the airbag sealing component 64 is working, the annular airbag 642 can be connected to the air source pipe, one end of which passes through the storage cabin 61 and is connected to the air source system. When the movable partition 63 reaches the specified position, the valve on the air source system is opened, and the air source system inputs pressurized gas into the annular airbag 642, and the annular airbag 642 expands to block the gap, and at the same time, pressure is applied to the friction cloth 643, so that the friction cloth 643 is in close contact with the inner wall of the storage cabin 61, so as to limit the movable partition 63 by friction. When the refrigeration component 65 is working, the liquid nitrogen evaporator 652 is connected to the refrigeration system through the liquid inlet pipe 653 and the liquid discharge pipe 654. The semiconductor refrigeration plate 651 and the liquid nitrogen evaporator 652 can both perform cooling work. The semiconductor refrigeration plate 651 can cool down after being powered on, which is suitable for maintaining the temperature requirements of four degrees Celsius and minus twenty degrees. The liquid nitrogen evaporator 652 is suitable for maintaining minus eighty degrees and the temperature required for continuous cell preservation.

[0028] Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the invention. The specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions and variations to the embodiments without creative contributions as needed without departing from the principles and purpose of the present invention. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A cell extraction and storage device for flow cytometric detection, comprising a test tube guide device (10) embedded on a workbench and located at the bottom of a flow cytometric detector discharge end, a base box (20) disposed on the workbench and located on one side of the test tube guide device (10), and a self-shrinking carrier (30) for clamping a plurality of test tubes, characterized in that: The base box (20) is provided with a carrier pulling and transposing device (40), a carrier transferring device (50) and a carrier freezing device (60) in sequence; The carrier pulling and transposing device (40) comprises a rotating component (41) arranged on the base box (20) and a pulling component (42) arranged on the workbench, the pulling component (42) being located on a side of the test tube guiding device (10) away from the base box (20), the rotating component (41) being used to move the self-shrinking carrier (30) to the test tube guiding device (10) and the rotating component (41) of the carrier transfer device (50), the pulling component (42) being used to stretch the self-shrinking carrier (30), and the test tube guiding device (10) being used to guide a test tube containing a sample into the stretched self-shrinking carrier (30); The carrier freezing device (60) comprises a plurality of storage compartments (61) arranged in a linear array on the base box (20), an electromagnetic door (62) arranged at the end of the storage compartment (61), a movable partition (63) arranged in the storage compartment (61), and a refrigeration component (65) embedded in the movable partition (63); The carrier rack transfer device (50) is used to move the self-shrinking carrier rack (30) into the carrier rack freezing device (60).

2. A cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The self-shrinking carrier (30) comprises a first carrier plate (31), a second carrier plate (32) slidably connected to the bottom of the first carrier plate (31), a third carrier plate (33) slidably connected to the bottom of the second carrier plate (32), and a fourth carrier plate (34) slidably connected to the bottom of the third carrier plate (33); The first bearing plate (31) is provided with a circular through hole (311), the second bearing plate (32), the third bearing plate (33) and the fourth bearing plate (34) are all provided with a U-shaped through hole (321), and the outer walls of the first bearing plate (31), the second bearing plate (32) and the third bearing plate (33) are all provided with an arc-shaped groove (312); A first vertical plate (313) is provided at one end of the first supporting plate (31), and a second vertical plate (314) is provided at one side; a third vertical plate (341) is provided at one end of the fourth supporting plate (34); U-shaped tubes (35) are provided on the outer walls of the first vertical plate (313) and the third vertical plate (341); and an elastic rope (36) is provided between the two U-shaped tubes (35).

3. A cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The rotating component (41) comprises a first micro motor (411) embedded in the base box (20), a rotating disk (412) disposed at the execution end of the first micro motor (411) and arranged horizontally, and a plurality of first electromagnetic blocks (413) disposed in an annular array on the outer wall of the rotating disk (412).

4. A cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The pulling component (42) comprises a micro-driving cylinder (421) arranged on a workbench, a pulling plate (422) arranged at an execution end of the micro-driving cylinder (421), and a second electromagnetic block (423) arranged at an end of the pulling plate (422).

5. The cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The test tube guiding device (10) comprises a positioning box (11) embedded on a workbench, a micro electric cylinder (12) whose linear array is arranged in the positioning box (11) and whose execution end penetrates the positioning box (11) and extends to the upper part of the positioning box (11), a suction cup (13) arranged at the execution end of the micro electric cylinder (12), and a negative pressure source tube (14) whose one end is connected to the suction cup (13).

6. A cell extraction and storage device for flow cytometry according to claim 1, characterized in that: An airbag sealing component (64) is provided at one end of the movable partition (63) away from the electromagnetic door (62), the airbag sealing component (64) comprising a support plate (641) provided on the outer wall of the movable partition (63), an annular airbag (642) provided on the outer wall of the support plate (641), and a friction cloth (643) provided on the outer wall of the movable partition (63) and located between the annular airbag (642) and the storage compartment (61).

7. The cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The refrigeration component (65) comprises a semiconductor refrigeration plate (651) and a liquid nitrogen evaporator (652) embedded in the movable partition (63), and a liquid inlet pipe (653) and a liquid discharge pipe (654) having one end penetrating the outer wall of the storage compartment (61) and connected to the liquid nitrogen evaporator (652).

8. The cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The base box (20) is provided with a power component (66) for driving the movable partition (63) to move, and the power component (66) includes a rotating shaft (661) connected to the upper surface of the base box (20) through a plurality of bearing seats, a power motor (662) provided on the base box (20) and used to drive the rotating shaft (661) to rotate, a plurality of electromagnetic rings (663) provided on the outer wall of the rotating shaft (661), a winch (664) sleeved on the outer wall of the electromagnetic ring (663), a connecting rope (665) having one end connected to the winch (664) and the other end passing through the storage cabin (61) and connected to the outer wall of the movable partition (63), and a spring (666) provided on the inner wall of the storage cabin (61) and used to provide a supporting force to the movable partition (63).

9. The cell extraction and storage device for flow cytometry according to claim 1, characterized in that: The carrier transfer device (50) comprises a first linear module (51) disposed in the base box (20) and having an execution end that passes through the base box (20) and extends to the upper part of the base box (20), an L-shaped plate (52) horizontally disposed at the execution end of the first linear module (51), a micro telescopic cylinder (53) disposed on the outer wall of the L-shaped plate (52) and having an execution end that passes through the L-shaped plate (52), an L-shaped electromagnetic block (54) disposed at the execution end of the micro telescopic cylinder (53), and an opening and closing component (55) disposed at the end of the L-shaped plate (52).

10. A cell extraction and storage device for flow cytometry detection according to claim 9, characterized in that: The opening and closing component (55) comprises an L-shaped frame (551) arranged at the end of the L-shaped plate (52), a second micro motor (552) arranged at the top of the L-shaped frame (551) and having an execution end passing through the L-shaped frame (551), and an opening and closing electromagnetic block (553) arranged at the execution end of the second micro motor (552).

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