Device for large-scale propagation of larva parasitic wasps and propagation method

Abstract

The device comprises a host containing cage and a parasitic wasp feeding cage, six faces of the host containing cage are nylon nets with a certain mesh number, the nylon net face of the top face of the host containing cage is provided with a zipper, and hosts can be led in and led out from the zipper; six surfaces of the parasitic wasp rearing cage are nylon nets with a certain mesh number, and the nylon net surface on the front surface is provided with a zipper; the internal positions of four top corners of the top surface of the parasitic wasp rearing cage are respectively provided with a rope hook with a certain length, and the rope hooks can be respectively hung at the external positions of the four top corners of the top surface of the parasitic wasp rearing cage, so that the host containing cage is horizontally suspended and hung in the parasitic wasp rearing cage; a certain distance is formed between the nylon net on the top surface of the host containing cage and the nylon net on the top surface of the parasitic wasp feeding cage. According to the device and the method, the parasitic process of the larva parasitic wasps can be simplified, the amount of labor used for parasitic operation is reduced, the propagation efficiency of the larva parasitic wasps is greatly improved, and the propagation cost of the larva parasitic wasps is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biological control of natural enemy insects, in particular to technologies related to large-scale breeding of natural enemy insects, and more particularly to a device and a breeding method for large-scale breeding of larval parasitic wasps. Background Art

[0002] Lepidoptera Noctuidae pests are widely distributed in various countries and regions. Representative pests include: Spodoptera lituraFabricius, Spodoptera frugiperda, Spodoptera exigua and Helicoverpa armigera. They have large appetite, varied diet and strong reproductive capacity. The larvae chew and cause holes or gaps in plant leaves, which may cause plant death in severe cases.

[0003] At present, the method of controlling Noctuidae pests is still mainly chemical control, but the long-term use of chemical pesticides has led to excessive pesticide residues, increased pest resistance, and the destruction of ecological balance. As an important part of biological control, the control of natural enemies of pests is a green and safe long-term pest control method. As natural enemies of pests, parasitic wasps are an important biological control resource. After large-scale production and packaging, they can be used as pest biological control products for green pest control, which has great application value.

[0004] Meteorus pulchricornis can parasitize Lepidoptera larvae, and plays an important role in the prevention and control of many Lepidoptera pests such as Spodoptera litura, Fall Armyworm, Beet Armyworm, and Cotton Bollworm, and has a high potential for biological control. The female Meteorus pulchricornis lays eggs in the body of Lepidoptera larvae, which rapidly reduce their food intake after being parasitized. After the parasitic wasp larvae mature in its body, they drill out of the Lepidoptera larvae and cocoon, and the Lepidoptera larvae eventually die. Summary of the invention

[0005] The technical problem solved by the invention is to provide a device and a breeding method for large-scale propagation of larval parasitic wasps.

[0006] The specific technical solutions are as follows:

[0007] A device for large-scale propagation of larval parasitic wasps, comprising a host holding cage and a parasitic wasp breeding cage, wherein the six sides of the host holding cage are all nylon nets with a certain mesh number, and the nylon net surface on the top surface thereof has a zipper for setting hosts to be introduced and led out, and the six sides of the parasitic wasp breeding cage are also nylon nets with a certain mesh number, and the nylon net surface on the front surface thereof has a zipper for setting the host holding cage to be put in and taken out, and the four top corners of the top surface of the parasitic wasp breeding cage have rope hooks with a certain length respectively at the inner positions, which can be respectively hung at the outer positions of the four top corners of the top surface of the parasitic holding cage to horizontally suspend the host holding cage in the parasitic wasp breeding cage, and the rope hooks with a certain length are arranged so that a certain distance exists between the top surface nylon net of the host holding cage and the top surface nylon net of the parasitic wasp breeding cage;

[0008] As a preferred embodiment, the invention comprises two parts: a host holding cage and a parasitic wasp breeding cage;

[0009] Preferably, the six sides of the host holding cage are all made of 25-30 mesh nylon mesh;

[0010] Preferably, the top nylon net of the host holding cage has a zipper for opening or closing;

[0011] Preferably, the zipper of the nylon net on the top surface of the parasitic wasp breeding cage is opened to allow the host to be introduced and led out;

[0012] Preferably, the four top corners of the top surface of the parasitic wasp breeding cage are respectively provided with hanging openings for hanging hooks;

[0013] Preferably, the six sides of the parasitic wasp breeding cage are all 100-200 mesh nylon mesh;

[0014] Preferably, the front nylon net of the parasitic wasp breeding cage has a zipper for opening or closing;

[0015] Preferably, the zipper of the nylon net on the front of the parasitic wasp breeding cage is opened to allow the host holding cage to be placed in or taken out;

[0016] Preferably, the four top corners of the top surface of the parasitic wasp breeding cage are each provided with a rope hook of a certain length;

[0017] Preferably, the four rope hooks of the parasitic wasp rearing cage can be hung on the hanging openings outside the four top corners of the top surface of the host holding cage, and the host holding cage can be horizontally suspended in the air and hung inside the parasitic wasp rearing cage;

[0018] Preferably, the host holding cage is hung at a position where the distance between the top nylon net of the host holding cage and the top nylon net of the parasitic wasp breeding cage is 3-5 cm;

[0019] Preferably, the size of the host holding cage is: a cube with a side length of 30-50 cm;

[0020] Preferably, the dimensions of the parasitic wasp breeding cage are: 20-40 cm in length, 20-40 cm in width, and 4-8 cm in height;

[0021] The breeding method for large-scale propagation of larval parasitic wasps provided by the present invention is specifically performed as follows:

[0022] (1) Rearing of larval parasitic wasps: Place the cocoons of parasitic wasps that are about to emerge into a parasitic wasp rearing cage, and feed them with cotton balls soaked in 10% honey water after they emerge. The cotton balls soaked in honey water should be replaced once a day.

[0023] (2) Parasitism process of larval parasitic wasps: Place hosts of appropriate age into a host holding cage, and then hang the host holding cage in a parasitic wasp breeding cage for 24 hours.

[0024] (3) Feeding of hosts after parasitism: After the parasitism is over, the host cage is removed from the parasitic wasp breeding cage; the hosts inside are then introduced into the host breeding tray for continued feeding, and artificial feed for the hosts is added every 2 days. On the 7th to 8th day after parasitism, the hosts that have not been parasitized are picked out.

[0025] (4) Collection of cocoons: On the 10th to 11th day after parasitism, the cocoons are collected from the host feeding tray and placed in a container for further cultivation.

[0026] The above steps (1) to (4) complete a parasitic process, thereby cycling the entire parasitic process to achieve the purpose of rapid proliferation of parasitic wasps. The specific effects are as follows:

[0027] a) It can quickly separate the host and the parasitic wasp. Compared with the traditional parasitism method (introducing and removing adult parasitic wasps from a single cage multiple times), the parasitism operation is more efficient and does not cause any damage to the parasitic wasps;

[0028] b) Compared with the existing propagation method, the breeding method using the device reduces the working hours and increases the cocoon recovery rate, and can significantly reduce the propagation cost of larval parasitic wasps. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A schematic diagram of the structure of a device for multiplying larval parasitic wasps provided by the present invention;

[0030] Figure 2 This is a schematic diagram of the relevant structure of the host holding cage;

[0031] Figure 3 This is a schematic diagram of the relevant structure of a parasitic wasp breeding cage.

[0032] Numbers in the figure: 1 host holding cage, 2 zipper of nylon net on the top surface of host holding cage, 3 hanging openings at 4 top corners of the top surface of host holding cage, 4 parasitic bee breeding cage, 5 zipper of nylon net on the front surface of parasitic bee breeding cage, 6 rope hooks at 4 top corners of the top surface of parasitic bee breeding cage. DETAILED DESCRIPTION

[0033] The present invention will be further described below in conjunction with the accompanying drawings and implementation modes.

[0034] like Figure 1-3 As shown, the present invention provides a device for propagating larval parasitic wasps, comprising a host holding cage 1 and a parasitic wasp breeding cage 4, wherein the top nylon net of the host holding cage 1 has a zipper 2, and the four top corners of the top surface respectively have hanging openings 3, the front nylon net of the parasitic wasp breeding cage 4 has a zipper 5, and the four top corners of the top surface respectively have rope hooks 6 of a certain length.

[0035] In a specific implementation manner, Figure 1-3 As shown, when the zipper 2 of the nylon net on the top surface of the host holding cage is opened, the host can be introduced into or led out of the host holding cage, and when the zipper 5 on the front nylon net of the parasitic bee breeding cage is opened, the host holding cage can be put into or taken out of the parasitic bee breeding cage, and the hanging openings 3 at the four corner positions of the top surface of the host holding cage can be connected with the rope hooks 6 at the four corner positions of the top surface of the parasitic bee breeding cage.

[0036] The device, when in use, comprises the following:

[0037] ① Rearing of larval parasitic wasps into adult bees: Place the cocoons of parasitic wasps that are about to emerge into parasitic wasp breeding cages, and feed them with cotton balls soaked in 10% honey water after they emerge. Replace the cotton balls with honey water once a day.

[0038] ② The parasitic process of larval parasitic wasps: Place the host of appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp breeding cage for parasitism for 24 hours.

[0039] ③ Feeding of hosts after parasitism: After the parasitism is over, take out the host cage from the parasitic wasp breeding cage; then introduce the hosts inside into the host breeding tray for continued feeding, add artificial feed to the hosts every 2 days, and pick out the hosts that have not been parasitized on the 7th-8th day after parasitism.

[0040] ④ Collection of cocoons: On the 10th-11th day after parasitism, collect the cocoons from the host feeding tray and place them in a container for further cultivation.

[0041] The above steps ① to ④ complete a parasitic process, thereby circulating the entire parasitic process to achieve the purpose of rapid expansion of parasitic wasps.

[0042] Example 1: Parasitism of Lycopodiella punctata on the rice moth

[0043] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was a 6th-instar larva of the rice moth.

[0044] (2) Host cage: a cubic insect cage of 30*30*5 cm, with 30 mesh nylon mesh on the six sides; parasitic wasp cage: a rectangular insect cage of 40*40*40 cm, with 100 mesh nylon mesh on the six sides.

[0045] (3) Operation process:

[0046] ① Rearing of adult larval parasitic wasps: Place 200 parasitic wasp cocoons that are about to emerge into a parasitic wasp breeding cage, and feed them with cotton balls soaked in 10% honey water after they emerge. Replace the cotton balls with honey water once a day.

[0047] ② The parasitic process of larval parasitic wasps: When the larval parasitic wasps are 4 days old, introduce 2,000 6-instar rice moth larvae of the appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp breeding cage for parasitism for 24 hours.

[0048] ③ Feeding of hosts after parasitism: After the parasitism is over, take out the host cage from the parasitic wasp breeding cage; then introduce the rice moth into the host breeding tray and continue to feed it, add artificial feed for the rice moth host every 2 days, and pick out the rice moth hosts that have not been parasitized on the 7th-8th day after parasitism.

[0049] ④ Collection of cocoons: On the 10th to 11th day after parasitism, collect the cocoons from the host feeding tray into a container, and count the number of rice moth larvae parasitized and the number of cocoons collected by the parasitic bees.

[0050] Parasitism: the number of rice moth larvae parasitized

[0051] Parasitism rate = number of parasites / total number of hosts

[0052] Cocoon yield: the number of parasitic wasp cocoons formed after the rice moth larvae are parasitized

[0053] Cocoon collection rate = cocoon collection amount / total host amount

[0054] This control treatment was repeated 5 times and the data were finally processed and analyzed.

[0055] Control 1: Parasitoids of the larvae Mylospermum oryzae

[0056] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was Cypripedium oryzae.

[0057] (2) Parasitic container: The parasitic cage 1 is a cubic insect cage of 40*40*40 cm, and the nylon mesh number of the 6 faces is 100 mesh.

[0058] (3) Operation process:

[0059] ① Feeding of adult larval parasitic wasps: 200 parasitic wasp cocoons that are about to emerge are placed in a parasitic cage 1, and after they emerge, they are fed with a cotton ball soaked in 10% honey water, and the cotton ball soaked in honey water is replaced once a day.

[0060] ② The parasitic process of larval parasitic wasps: When the larval parasitic wasps are 4 days old, 2000 6-instar rice moth larvae of appropriate age are introduced into the parasitic cage 1 and parasitized for 24 hours.

[0061] ③ Feeding of hosts after parasitism: After the parasitism is over, take out the rice moth larvae in the parasitic cage 1 one by one; place the rice moth in the host feeding tray and continue to feed it, add artificial feed for the rice moth host every 2 days, and pick out the rice moth hosts that have not been parasitized on the 7th to 8th day after parasitism.

[0062] ④ Collection of cocoons: On the 10th to 11th day after parasitism, collect the cocoons from the host feeding tray into a container, and count the number of rice moth larvae parasitized and the number of cocoons collected by the parasitic bees.

[0063] Parasitism: the number of rice moth larvae parasitized

[0064] Parasitism rate = number of parasites / total number of hosts

[0065] Cocoon yield: the number of parasitic wasp cocoons formed after the rice moth larvae are parasitized

[0066] Cocoon collection rate = cocoon collection amount / total host amount

[0067] This control treatment was repeated 5 times and the data were finally processed and analyzed.

[0068] By comparing Example 1 with Control Example 1, it can be seen that: (1) the parasitism rate of the expanded mole-shaped bracone in Example 1 is higher; (2) the cocoon collection rate of the parasitic bees of the expanded mole-shaped bracone in Example 1 is higher; (3) the working hours used in the parasitic bee expansion method in Example 1 are about 67% of that in Control Example 1, thereby reducing the labor cost.

[0069] Table 1 Comparison of propagation results of the schizophora punctata using the larvae as hosts

[0070]

[0071]

[0072] Example 2: Parasitic Mythimna separasitized by Dinocampus punctatus

[0073] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was the second-instar larvae of the armyworm.

[0074] (2) Host cage: a cubic insect cage of 25*25*5 cm, with 25 mesh nylon mesh on the six sides; parasitic wasp cage: a rectangular insect cage of 35*35*35 cm, with 120 mesh nylon mesh on the six sides.

[0075] (3) Operation process:

[0076] ① Rearing of adult larval parasitic wasps: Place 150 parasitic wasp cocoons that are about to emerge into a parasitic wasp breeding cage, and feed them with cotton balls soaked in 10% honey water after they emerge. Replace the cotton balls with honey water once a day.

[0077] ② The parasitic process of larval parasitic wasps: When the larval parasitic wasps are 4 days old, introduce 1500 2nd instar armyworm larvae of appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp breeding cage for parasitism for 24 hours.

[0078] ③ Feeding of hosts after parasitism: After the parasitism is over, take out the host cage from the parasitic wasp breeding cage; then introduce the sticky insects into the host breeding tray to continue feeding, add artificial feed for the sticky insect hosts every 2 days, and pick out the sticky insect hosts that have not been parasitized on the 7th-8th day after parasitism.

[0079] ④ Collection of cocoons: On the 10th to 11th day after parasitism, collect the cocoons from the host feeding tray into a container, and count the number of armyworm larvae parasitized and the number of cocoons collected by parasitic bees.

[0080] Parasitism: the number of armyworm larvae parasitized

[0081] Parasitism rate = number of parasites / total number of hosts

[0082] Cocoon yield: the number of cocoons formed by parasitic wasps after the armyworm larvae are parasitized

[0083] Cocoon collection rate = cocoon collection amount / total host amount

[0084] This control treatment was repeated 5 times and the data were finally processed and analyzed.

[0085] Control 2: Parasitoids of Myxomyces pilosa

[0086] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was Mythida separata.

[0087] (2) Parasitic container: The parasitic cage 2 is a cubic insect cage of 35*35*35 cm, and the nylon mesh number of the 6 faces is 120 mesh.

[0088] (3) Operation process:

[0089] ① Feeding of adult larval parasitic wasps: 150 parasitic wasp cocoons that are about to emerge are placed in a parasitic cage 2, and after they emerge, they are fed with a cotton ball soaked in 10% honey water, and the cotton ball soaked in honey water is replaced once a day.

[0090] ② The parasitic process of larval parasitic wasps: When the larval parasitic wasps are 4 days old, 1500 second-instar sticky insect larvae of appropriate age are introduced into parasitic cage 2 and parasitized for 24 hours.

[0091] ③ Host feeding after parasitism: After the parasitism is over, take out the armyworm larvae in the parasitic cage 2 one by one; place the armyworms in the host feeding tray and continue to feed them, add artificial feed for the armyworm hosts every 2 days, and pick out the armyworm hosts that have not been parasitized on the 7th to 8th day after parasitism.

[0092] ④ Collection of cocoons: On the 10th to 11th day after parasitism, collect the cocoons from the host feeding tray into a container, and count the number of armyworm larvae parasitized and the number of cocoons collected by parasitic bees.

[0093] Parasitism: the number of armyworm larvae parasitized

[0094] Parasitism rate = number of parasites / total number of hosts

[0095] Cocoon yield: the number of cocoons formed by parasitic wasps after the armyworm larvae are parasitized

[0096] Cocoon collection rate = cocoon collection amount / total host amount

[0097] This control treatment was repeated 5 times before data processing and analysis.

[0098] By comparing Example 2 with Control Example 2, it can be seen that: (1) the parasitism rate of the expanded mole-shaped bracone in Example 2 is also higher; (2) the cocoon collection rate of the parasitic bees of the expanded mole-shaped bracone in Example 2 is also higher; (3) the working hours used in the parasitic bee expansion method in Example 2 are about 61% of the method in Control Example 2, which reduces the labor cost.

[0099] Table 2 Comparison of propagation results of Myxomyces punctatus using myxomycetes as hosts

[0100]

[0101]

[0102] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A device for scaling up the reproduction of larval parasitic wasps, characterized in that: The utility model comprises two parts: a host holding cage and a parasitic wasp breeding cage.

2. The device for scaling up larval parasitic wasps according to claim 1, characterized in that: The top nylon net of the host holding cage is provided with a zipper for opening or closing.

3. The device for scaling up larval parasitic wasps according to claim 2, characterized in that: The zipper of the nylon net on the top surface of the parasitic wasp breeding cage is opened so that the host can be introduced and led out.

4. The device for scaling up larval parasitic wasps according to claim 2, characterized in that: The four top corners of the top surface of the parasitic wasp breeding cage are respectively provided with hanging openings for hanging hooks.

5. The device for scaling up larval parasitic wasps according to claim 1, characterized in that: The six sides of the parasitic wasp breeding cage are all nylon nets with 100-200 meshes.

6. The device for scaling up larval parasitic wasps according to claim 5, characterized in that: The parasitic wasp rearing cage has a zipper on the front nylon net for opening or closing.

7. The device for scaling up larval parasitic wasps according to claim 6, characterized in that: The host holding cage can be put in or taken out when the zipper of the nylon net on the front side of the parasitic wasp breeding cage is opened.

8. The device for scaling up larval parasitic wasps according to claim 1, characterized in that: The inner positions of the four top corners of the top surface of the parasitic wasp breeding cage are respectively provided with rope hooks of a certain length.

9. The device for scaling up larval parasitic wasps according to claim 8, characterized in that: The four rope hooks of the parasitic wasp breeding cage can be hung on the hanging openings outside the four top corners of the top surface of the host containing cage, and the host containing cage can be horizontally suspended in the parasitic wasp breeding cage.

10. The device for scaling up larval parasitic wasps according to claim 9, characterized in that: The hanging position of the host holding cage is such that the distance between the top nylon net of the host holding cage and the top nylon net of the parasitic wasp breeding cage is 3-5 cm.

11. The device for scaling up larval parasitic wasps according to claim 1, characterized in that: The size of the host holding cage is a cube with a side length of 30-50 cm.

12. The device for scaling up larval parasitic wasps according to claim 1, characterized in that: The dimensions of the parasitic wasp breeding cage are: 20-40 cm in length, 20-40 cm in width, and 4-8 cm in height.

13. A breeding method, characterized in that: The method of breeding larval parasitic wasps using the device for breeding larval parasitic wasps according to any one of claims 1 to 12 comprises the following steps: Feeding of adult parasitic wasps from larvae: Place the cocoons of parasitic wasps that are about to emerge into the parasitic wasp breeding cage, and feed them with cotton balls soaked in 10% honey water after they emerge. Change the cotton balls soaked in honey water once a day. The parasitism process of larval parasitic wasps: put the host of appropriate age into the host holding cage, and then hang the host holding cage in the parasitic wasp breeding cage to parasitize for 24 hours; Feeding of hosts after parasitism: After parasitism is over, take out the host cage from the parasitic wasp breeding cage; then introduce the hosts in it into the host breeding tray for continued feeding, add artificial feed for the hosts every 2 days, and pick out the hosts that have not been parasitized on the 7th to 8th day after parasitism; Collection of cocoons: On the 10th to 11th day after parasitism, collect the cocoons from the host feeding tray and place them in a container for further cultivation.

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