Microalgae culture device coupled with air purification and fertilizer preparation and use method of microalgae culture device

Abstract

The invention relates to a microalgae culture device coupling air purification and fertilizer preparation and a using method thereof.The microalgae culture device comprises a cylinder cover, a cylinder body and a base, the interior of the cylinder body is divided into at least two culture cavities in the circumferential direction, a lamp cavity for containing an auxiliary lamp is formed in the middle of the cylinder body, and a liquid outlet hole with a liquid discharging valve is formed in the bottom of each culture cavity; a sealed cover plate and a fixing ring are arranged at the top of the cylinder body, the fixing ring is in threaded fixation with the top of the cylinder body and clamps the cover plate, exhaust holes and air inlet pipes which are communicated with the culture cavities are integrally arranged on the plate surface of the cover plate, an air pump connected with the air inlet pipes is fixedly arranged in the cylinder cover, and air outlet holes communicated with the exhaust holes are formed in the cylinder cover; and the bottom of the cylinder body is spliced and matched with the base. During use, microalgae liquid is put into the culture cavity of the cylinder body, the whole body is placed at an illumination position or an auxiliary lamp is turned on, and meanwhile, an air pump is turned on, so that microalgae culture and air purification can be realized, and the use is relatively convenient; after being cultured, the microalgae are discharged to the base to be used as a fertilizer, so that the comprehensive utilization rate is high.

Description

Technical Field

[0001] The invention relates to the field of microalgae cultivation and air purification, and is a microalgae cultivation device coupled with air purification and fertilizer preparation and a use method thereof. Background Art

[0002] With the development of urbanization, a large number of people, transportation, factories, etc., the air quality is relatively poor and the air freshness is low. Coupled with the frequent smog weather in autumn and winter, the air quality of the home environment is worrying, affecting people's physical and mental health. With the advancement of technology, most people now choose to use air purifiers to maintain the quality of indoor air. However, most traditional air purifiers only have adsorption functions and cannot increase the oxygen production of air to increase the oxygen content. At the same time, a certain amount of energy consumption is required to maintain the operation of the fan, and it is difficult to avoid noise during operation.

[0003] Microalgae are ancient organisms that have powerful photosynthetic pigments that can capture carbon dioxide and release oxygen through photosynthesis. It is reported that the photosynthesis efficiency of microalgae is 10-50 times that of terrestrial plants. Microalgae are liquid cultures that can maintain indoor humidity and avoid the problem of dry air. At the same time, microalgae also have the characteristics of short growth cycle and strong adsorption of harmful substances and suspended particles. These characteristics make microalgae have great development potential in the field of air purification.

[0004] In addition, microalgae also has the following advantages: 1. Microalgae can produce a large number of biologically active substances, which can help plant growth to a large extent; 2. The composition of microalgae itself can also provide a large amount of nitrogen, phosphorus, potassium and other essential elements for plant growth; 3. Microalgae can promote the formation of soil multi-ecosystem, improve soil structure, and increase soil fertility and permeability. Therefore, in recent years, microalgae has been regarded as a new source of plant fertilizer.

[0005] In view of the drawbacks of current air purifiers, the prior art has disclosed the use of microalgae to replace traditional purification devices. For example, Chinese patent CN209362223U discloses a cylindrical microalgae air purification device, and combines activated carbon, filter cotton and other auxiliary air purification. Chinese patent CN209362222U discloses a chair-shaped microalgae air purification system, in which the microalgae cultivation device is arranged in a square box shape and placed on the top of the device, making the appearance more beautiful and easy to clean. Chinese patent CN209355397U discloses a self-moisturizing microalgae air purifier, in which microalgae are applied to PVA materials and combined with activated carbon layers to form a multi-layer biological purification system, and water-absorbing materials are used to provide the moisture required for the growth of microalgae. However, these microalgae air purifiers basically only focus on the purification function of microalgae, not on cultivation, making microalgae consumables, and not maximizing the value of microalgae. At the same time, most microalgae purification equipment is large in size and relatively complex in structure, which is not suitable for use in a small space. In addition, most microalgae air purifiers can only cultivate a single type of microalgae, which not only has a low cultivation efficiency but also has a low overall purification efficiency.

[0006] Therefore, it is necessary to improve the existing microalgae air purifiers to improve the air purification efficiency and increase the utilization of microalgae. Summary of the invention

[0007] In order to overcome the above shortcomings, the purpose of the present invention is to provide a microalgae cultivation device and a method of using the device for coupling air purification and fertilizer preparation to the art, so as to solve the technical problems that the existing similar microalgae purification equipment is large in size, the microalgae cultivation is relatively single, the comprehensive purification efficiency is not high, and the microalgae cultivation and maximization of microalgae utilization are not emphasized. The purpose is achieved through the following technical solutions.

[0008] A microalgae cultivation device coupled with air purification and fertilizer preparation, the microalgae cultivation device comprises a cylinder cover, a cylinder body and a base, at least one side of the cylinder body is transparent; it is characterized in that at least two cultivation chambers are formed by circumferential division in the cylinder body, a transparent lamp chamber for placing an auxiliary lamp is provided in the middle of the cylinder body, each cultivation chamber of the cylinder body is provided with a liquid outlet at the bottom, a liquid discharge valve is provided at the liquid outlet, a sealed cover plate and a fixing ring are provided at the top of the cylinder body, the fixing ring is threadedly fixed to the top of the cylinder body and clamps the cover plate, a sealing gasket is provided at the bottom of the cover plate to seal and separate the top of each cultivation chamber in the cylinder body from the top of the lamp chamber, and the cover plate The plate surface is integrated with exhaust holes and air inlet pipes that communicate with each culture chamber, wherein the air inlet pipe extends into the bottom of the culture chamber, the bottom of the cylinder cover and the top of the fixing ring are plug-in sealed, an air pump is fixed inside the cylinder cover, the air inlet of the air pump communicates with the outside of the cylinder cover, and the air outlet of the air pump is branched to connect the air inlet pipes of the cover plate, the cylinder cover is provided with an air outlet hole for guiding the gas discharged from the exhaust hole of the cover plate to the outside of the cylinder cover, and the cylinder cover is integrated with a light-controlled switch and an air pump switch for controlling the operation of the auxiliary lamp and the air pump; the bottom of the cylinder body is plug-in matched with the base, and a material receiving chamber is formed inside the base. Through the above structure, it is possible to cultivate multiple microalgae in the cylinder at the same time, which not only realizes the effective cultivation of microalgae, but also utilizes the photosynthesis of microalgae to achieve effective air purification. When different microalgae are matched, the effect of comprehensive air purification can be effectively improved. After the cultivation of microalgae is completed, the algae liquid can be discharged into the material receiving chamber of the base in time, and the algae liquid can be used as fertilizer, with a high comprehensive utilization rate. In addition, the overall structure is quick and easy to assemble and use, making it convenient for cleaning and fluid replacement operations.

[0009] The exhaust hole of the cover plate is provided with a thread, and is threadedly connected to a one-way drain valve, which includes a valve body and a valve core. The valve body is provided with a gas-liquid port that conducts inside and outside of the cover plate, and the inner end of the valve body is provided with a hollow bracket that limits the valve core, and the valve core is limited in the hollow bracket; when the cylinder is vertical, under the dead weight of the valve core, an exhaust gap is formed between the valve core and the gas-liquid port, and the gas-liquid port is in an open state; when the valve core is pressed against the gas-liquid port by an external force, the gas-liquid port is closed. Through this structure, a large amount of algae liquid is effectively prevented from overflowing from the exhaust hole when the cylinder is tipped over or overturned, which has an effective algae liquid overflow prevention effect, and is safer and more convenient to use.

[0010] The drain valves of each outlet hole at the bottom of the cylinder body include a valve head, a valve seat, a spring, and a manual switch plate. The valve head is in an inverted cone shape and is limited to the top of the outlet hole. The valve seat includes a valve stem and a valve plate connected as one. The top of the valve stem is threadedly connected to the valve head. The spring is sleeved on the valve stem, and the spring is tightly limited to be located between the bottom surface of the cylinder body and the valve plate. Under the action of the spring, the valve head and the outlet hole form a seal. The manual switch plate is hinged to the hinge seat integrated at the bottom of the cylinder body. One end of the manual switch plate extends out of the opening of the bottom surface of the cylinder body, and the other end of the manual switch plate is located at the bottom of the valve seat. When the manual switch plate is pressed, the manual switch plate pushes the valve seat to drive the valve head and the outlet hole to release the sealing state. Through this structure, the manual drain operation of the culture chamber in the cylinder body is realized, and the drain speed and the amount of liquid can be effectively adjusted according to the manual force, so that different microalgae components can be prepared in the base according to needs to achieve the required fertilizer requirements.

[0011] The volume of the receiving cavity in the base is not less than the volume of one of the culture cavities in the cylinder. This structure makes it convenient to receive and use the materials in the base.

[0012] The base is transparent, and through this structure, it is convenient to observe the material connection situation of the base.

[0013] The auxiliary lamp is a color-changing LED lamp, and is provided with remote control and timing control. Through this structure, the use of the LED lamp is convenient, and the color change is conducive to the cultivation of different microalgae.

[0014] The lamp cavity is convex relative to the culture cavity, and the convex part positions the cover plate. Through this structure, the assembly and connection between the cover plate and the cylinder body are more stable and reliable.

[0015] The side of the cylinder is provided with scales to display the changes in the solutions in each culture chamber. This structure makes it easy to observe the changes in the volume of the algae solution in each culture chamber, and also makes it easy for the base to receive the required mixed solution in the chamber.

[0016] The method for using the microalgae cultivation device coupled with air purification and fertilizer preparation is as follows: first, the cylinder is plugged into the base, and the algae liquid to be cultivated is injected into the cultivation chamber of the cylinder; then, the cover plate is matched with the top of the cylinder, and the cover plate is threadedly connected to the top of the cylinder through a fixing ring to lock the cover plate, so that the cover plate forms a sealed partition between the top of each cultivation chamber of the cylinder and the lamp chamber; next, the auxiliary lamp connected to the cylinder cover is inserted into the lamp chamber, the air outlet of the air pump is sealed and connected to the air inlet pipe of the cover plate through a branch joint and a hose, and then the cylinder cover is plugged into the top of the fixing ring. The installation of the microalgae cultivation device is completed by sealing; the microalgae cultivation device is placed in a lighted position or an auxiliary light is turned on, and an air pump is used to introduce external air into the bottom of the cultivation chamber. The microalgae in the algae liquid purifies the air and converts carbon dioxide into oxygen through photosynthesis. The oxygen is discharged to the outside through the exhaust holes of the cover plate and the exhaust holes of the cylinder cover to purify the air; after the microalgae cultivation is completed, the algae liquid is discharged into the material receiving chamber of the base through the liquid discharge valve at the bottom of the cylinder body, and the base is removed, so that the algae liquid in the base can be used as fertilizer to directly fertilize the crops.

[0017] The overall structure of the present invention is easy to assemble and use, and multiple groups of microalgae can be cultivated simultaneously. The use of different microalgae can effectively improve the effect of comprehensive air purification. The cultivated microalgae can also be conveniently taken out and directly used as fertilizer, which effectively improves the value of comprehensive utilization of microalgae. The present invention is suitable for use as an integrated device for cultivating, purifying and preparing fertilizers for microalgae, or a structural improvement of similar purification devices. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the explosion structure of the present invention.

[0019] Figure 2 yes Figure 1 Schematic diagram of the cylinder structure.

[0020] Figure 3 yes Figure 1 Schematic diagram of the assembly structure of the one-way discharge valve.

[0021] Figure 4 yes Figure 3 Schematic diagram of the assembled structure.

[0022] Figure 5 yes Figure 1 Schematic diagram of the valve head and valve seat structure of the liquid drain valve.

[0023] Figure 6 yes Figure 5 Schematic diagram of the structure of the middle valve head and valve seat assembly.

[0024] Figure 7 yes Figure 6 Schematic diagram of the structure of the liquid discharge valve combined with the manual switch plate.

[0025] Figure 8 It is a schematic diagram of the structure of the present invention in use state.

[0026] Fig. 9 is the dry biomass of microalgae cultured in Example 1 of the present invention.

[0027] Fig.10 is the cell number of the microalgae cultured in Example 1 of the present invention.

[0028] Fig.11 It is the dry biomass of microalgae cultured in Example 2 of the present invention.

[0029] Fig.12 This is the number of cells of the microalgae cultured in Example 2 of the present invention.

[0030] Fig.13 It is the dry biomass of microalgae cultured in Example 3 of the present invention.

[0031] Fig.14 is the carbon fixation amount of the microalgae cultured in Example 1 of the present invention.

[0032] Fig.15 is the carbon fixation amount of the microalgae cultured in Example 2 of the present invention.

[0033] Fig.16 is the carbon fixation amount of the microalgae cultured in Example 3 of the present invention.

[0034] Fig.17 is the oxygen release of the microalgae cultured in Example 1 of the present invention.

[0035] Fig.18 is the oxygen release of the microalgae cultured in Example 2 of the present invention.

[0036] Fig.19 is the oxygen release of the microalgae cultured in Example 3 of the present invention.

[0037] The serial numbers and names in the figure are: 1. cylinder body, 101. culture chamber, 102. lamp chamber, 103. liquid outlet, 104. opening, 105. hinged seat, 2. liquid discharge valve, 201. valve head, 202. valve stem, 203. valve plate, 204. spring, 3. manual switch plate, 4. base, 401. material receiving chamber, 5. cover plate, 501. sealing gasket, 502. exhaust hole, 503. air inlet pipe, 504. positioning hole, 6. one-way drain valve, 601. valve body, 6011. gas-liquid port, 6012. hollow bracket, 602. valve core, 7. fixing ring, 8. air pump, 801. branch joint, 9. auxiliary lamp, 10. cylinder cover, 1001. air inlet, 1002. air pump switch, 1003. light-controlled switch, 1004. air outlet. Implementation

[0039] The present invention will now be further described with reference to the accompanying drawings.

[0040] like Figure 1-Figure 8 As shown, the microalgae cultivation device coupled with air purification and fertilizer preparation comprises a cylinder cover 10, a cylinder body 1 and a base 4. The cylinder body is made of a transparent material as a whole. Three culture chambers 101 are formed by evenly dividing the cylinder body along the circumferential direction. The side of the cylinder body is provided with scales for displaying the changes of the solutions in each culture chamber. The middle of the cylinder body is provided with a lamp chamber 102 for placing an auxiliary lamp 9. The auxiliary lamp is a color-changing LED lamp, and is provided with a remote control and a timing control. The lamp chamber is raised relative to the top of the culture chamber. A liquid outlet 103 is provided at the bottom of each cavity of the cylinder body, and a liquid discharge valve 2 is provided at the liquid outlet. The liquid discharge valves all include a valve head 201, a valve seat, a spring 204, and a manual switch plate 3. The valve head is in an inverted cone shape and is limited to the top of the liquid outlet. The valve seat includes a valve stem 202 and a valve plate 203 that are connected as one. The top of the valve stem is threadedly connected to the valve head. The spring is sleeved on the valve stem, and the spring is tightly limited to between the bottom surface of the cylinder and the valve plate. Under the action of the spring, the valve head and the liquid outlet are sealed; the manual switch plate is hinged to the hinge seat 105 integrated at the bottom of the cylinder, one end of the manual switch plate extends out of the opening 104 on the bottom surface of the cylinder, and the other end of the manual switch plate is located at the bottom of the valve seat, that is, when the manual switch plate is pressed, the manual switch plate uses the lever principle to push the valve seat to drive the valve head to separate from the liquid outlet, that is, release the sealing state.

[0041] A sealed cover plate 5 and a fixing ring 7 are provided on the top of the cylinder 1. The fixing ring is threadedly fixed to the top of the cylinder and clamps the cover plate. A sealing gasket 501 is provided at the bottom of the cover plate for sealing and separating the top of each culture chamber 101 in the cylinder from the top 102 of the lamp chamber. The cover plate surface is integrated with exhaust holes 502 and air inlet pipes 503 communicating with each culture chamber, as well as a positioning hole 504 formed with a protruding part of the top of the lamp chamber relative to the culture chamber, wherein the air inlet pipe extends to the bottom of the culture chamber. The bottom of the above-mentioned cylinder cover 10 and the top of the fixing ring are plug-in and sleeved with a sealing ring to form a seal. An air pump 8 is fixedly arranged inside the cylinder cover. The air inlet of the air pump is communicated with the air inlet hole 1001 of the cylinder cover through a hose. The air outlet of the air pump is connected to a four-way branch joint 801. The three ports of the branch joint are respectively connected to the three sealing sockets on the top of the air inlet pipes of the cover plate through hoses; an air outlet hole 1004 communicating with the exhaust hole of the cover plate is arranged on the top of the cylinder cover, and a light-controlled switch 1003 and an air pump switch 1002 for controlling the auxiliary light 9 and the operation of the air pump are integrated on the side of the cylinder cover; the bottom of the cylinder body and the top of the base 4 are plug-in matched, and a material receiving chamber 401 is formed inside the base. The base is preferably made of transparent material to facilitate observation of the interior. The volume of the material receiving chamber is not less than the volume of one of the culture chambers in the cylinder body.

[0042] Furthermore, in order to prevent the algae liquid in the culture chamber 101 from pouring out after the cylinder 1 is tilted and turned over, the exhaust hole 502 of the above-mentioned cover plate 5 is provided with a thread and is threadedly connected to the one-way drain valve 6. The one-way drain valve includes a valve body 601 and a valve core 602. The valve body is provided with a gas-liquid port 6011 that connects the inside and outside of the cover plate. The upper end of the valve body is provided with a hollow bracket 6012 for limiting the valve core. The valve core is a rubber body and is inserted into the hollow bracket from the opening of the hollow bracket to be limited in the hollow bracket. When the cylinder is vertical, under the dead weight of the valve core, an exhaust gap is formed between the valve core and the gas-liquid port, and the gas-liquid port is in an open state. When the cylinder is tilted, the valve core is pressed against the gas-liquid port by the thrust of the algae liquid, and the gas-liquid port is closed to prevent the algae liquid from flowing out of the gas-liquid port in large quantities.

[0043] The method of using the microalgae cultivation device for coupled air purification and fertilizer preparation is as follows: first, the barrel 1 is plugged into the base 4, and the algae liquid to be cultivated is injected into the cultivation chamber 101 of the barrel. The three cultivation chambers can cultivate the same or different microalgae, and different microalgae have different purification effects; then the cover plate 5 is matched with the top of the barrel, and the cover plate is threadedly connected and locked with the top of the barrel through the fixing ring 7, and the cover plate forms a sealed separation between the top of each cultivation chamber of the barrel and the lamp chamber 102; next, the auxiliary lamp 9 connected to the barrel cover is inserted into the lamp chamber, and the air outlet of the air pump 8 is sealed and connected to the air inlet pipe 503 of the cover plate through the branch joint 801 and the hose, and then the barrel cover 10 is plugged and sealed with the top of the fixing ring, and the installation of the microalgae cultivation device is completed. The microalgae cultivation device is placed in a lighting position or an auxiliary light is turned on for illumination. An air pump is used to introduce external air into the bottom of the cultivation chamber. The microalgae in the algae liquid purifies the air and converts carbon dioxide into oxygen through photosynthesis. The oxygen is discharged to the outside through the one-way drain valve 6 at the exhaust hole 502 of the cover plate and the air outlet 1004 of the cylinder cover to purify the air. After the microalgae cultivation is completed, the algae liquid is discharged into the receiving chamber 401 of the base through the drain valve 2 at the bottom of the cylinder. The base is removed, and the algae liquid in the base can be used as fertilizer to directly fertilize the crops.

[0044] like Figure 9-19 As shown, three specific examples of microalgae cultivation are cited to illustrate the cultivation and purification effects of the microalgae cultivation device.

[0045] Embodiment 1:

[0046] Place the mixed BG11 culture medium in each culture chamber of the cylinder. Use the Chlorella seed solution that has been cultured to the exponential phase, add the seed solution according to 10% of the volume of the culture medium, and ventilate and mix evenly. Adjust the LED light to white and the light intensity to 5000LUX. Ventilate and culture for 8 days. Take samples every 2 days to measure the dry biomass and cell count.

[0047] Dry biomass was used to evaluate the growth of Chlorella. The determination method is as follows: Take 20mL of algae liquid to determine the algae cell concentration. Centrifuge the obtained algae liquid at 9000rpm for 10min and discard the supernatant. Then resuspend it with RO water and centrifuge it, repeat 1-2 times to remove the salt and nutrients remaining on the surface of the algae. After centrifugation, transfer it to a weighing dish with a small amount of water and dry it for about 24 hours to constant weight, then transfer it to a dryer to cool to room temperature and weigh it (W2). Before this, wash the glass weighing dish and dry it at 90℃ for about 24 hours to ensure that the weighing dish reaches constant weight, transfer it to a dryer to cool to room temperature, and then weigh it with an analytical balance (W1). The actual algae cell concentration is calculated based on the difference between the two masses (W2-W1). The dry weight concentration of algae cells is X=(W2-W1) / V, where V is the sampling volume.

[0048] The number of algal cells is determined by hemocytometer. Place the algal solution on a hemocytometer and cover it with a cover glass. Place it on a microscope and adjust the field of view from low to high. The first time you adjust the field of view, you should be able to see the entire counting chamber, the second time you should be within the counting chamber, and the third time you should make sure that a 4×4 square grid is in the field of view. Count in this field of view, and count five squares in one counting chamber (four corner squares and one in the middle). When counting the double lines of the square grid, only count the corresponding side up, down, left, and right (the principle of counting the top but not the bottom, and counting the left but not the right), and only count one side in the middle of the double lines. Where N is the average number of microalgae in a square grid under a 40x objective lens of a microscope.

[0049] Embodiment 2:

[0050] Place the mixed ASW culture medium in each culture chamber of the cylinder. Use the Porphyridium seed solution that has been cultured to the exponential phase, add the seed solution according to 10% of the volume of the culture medium, and ventilate and mix evenly. Adjust the LED light to white and the light intensity to 5000LUX. Ventilate and culture for 8 days. Take samples every 2 days to determine the dry biomass and cell number.

[0051] Dry biomass was used to evaluate the growth of Porphyridium cruentum. The determination method is as follows: Take 20 mL of algae liquid to determine the algae cell concentration. Centrifuge the obtained algae liquid at 9000 rpm for 10 minutes and discard the supernatant. Then resuspend it with RO water and centrifuge it, repeat 1-2 times to remove the salt and nutrients remaining on the surface of the algae. After centrifugation, transfer it to a weighing dish with a small amount of water and dry it for about 24 hours to constant weight, then transfer it to a dryer to cool to room temperature and weigh it (W2). Before that, wash the glass weighing dish and dry it at 90℃ for about 24 hours to ensure that the weighing dish reaches constant weight, transfer it to a dryer to cool to room temperature, and then weigh it with an analytical balance (W1). The actual algae cell concentration is calculated based on the difference between the two masses (W2-W1). The dry weight concentration of algae cells is X=(W2-W1) / V, where V is the sampling volume.

[0052] The number of algal cells is determined by hemocytometer. Place the algal solution on a hemocytometer and cover it with a cover glass. Place it on a microscope and adjust the field of view from low to high. The first time you adjust the field of view, you should be able to see the entire counting chamber, the second time you should be within the counting chamber, and the third time you should make sure that a 4×4 square grid is in the field of view. Count in this field of view, and count five squares in one counting chamber (four corner squares and one in the middle). When counting the double lines of the square grid, only count the corresponding side up, down, left, and right (the principle of counting the top but not the bottom, and counting the left but not the right), and only count one side in the middle of the double lines. Where N is the average number of microalgae in a square grid under a 40x objective lens of a microscope.

[0053] Embodiment 3:

[0054] Place the mixed Zarrouk medium in each culture chamber of the cylinder. Use the Spirulina seed solution that has been cultured to the exponential phase, add the seed solution according to 10% of the volume of the culture medium, and ventilate and mix evenly. Adjust the LED light to white and the light intensity to 5000LUX. Culture with ventilation for 10 days. Take samples every 2 days to determine the dry biomass and cell number.

[0055] Dry biomass was used to evaluate the growth of Spirulina. The determination method is as follows: Take 20mL of algae liquid to determine the algae cell concentration. Centrifuge the obtained algae liquid at 9000rpm for 10min and discard the supernatant. Then resuspend it with RO water and centrifuge it, repeat 1-2 times to remove the salt and nutrients remaining on the surface of the algae. After centrifugation, transfer it to a weighing dish with a small amount of water and dry it for about 24 hours to constant weight, then transfer it to a dryer to cool to room temperature and weigh it (W2). Before that, wash the glass weighing dish and dry it at 90℃ for about 24 hours to ensure that the weighing dish reaches constant weight, transfer it to a dryer to cool to room temperature, and then weigh it with an analytical balance (W1). The actual algae cell concentration is calculated based on the difference between the two masses (W2-W1). The dry weight concentration of algae cells is X=(W2-W1) / V, where V is the sampling volume.

[0056] Spirulina is not suitable for cell counting due to its size, so cell counting of Spirulina is not performed here.

[0057] The culture results of Examples 1-3 are: Figure 9-13 After 8 days of cultivation, the final dry biomass of Chlorella was 1.5 g / L, and the number of algal cells was 20.2×10 6 / mL; the final dry biomass of Porphyridium cruentum was 1.63g / L, and the number of algal cells was 5.6×10 6After 10 days of cultivation, the final dry biomass of Spirulina was 1.1 g / L. Compared with the current open microalgae cultivation, the results of the cultivation of three microalgae in the present invention are relatively good, indicating that the present invention has a better ability to cultivate microalgae.

[0058] like Figure 14-Figure 19 As shown, the ability of cultivated microalgae to purify the air is characterized by calculating the amount of carbon fixed and the amount of oxygen released. On average, 1 gram of algae synthesized by microalgae can absorb and fix 1.83 grams of carbon dioxide. At the same time, the plant photosynthesis equation shows that 1 mole of carbon dioxide is absorbed and the same number of moles of oxygen is released. Therefore, 1 gram of algae produced by microalgae can dilute 0.042 moles of oxygen, that is, 1.34 grams of oxygen. According to this method, the amount of carbon dioxide fixed and the amount of oxygen produced by the device are calculated. When the indoor air enters the device from the air inlet of the tube cover, it is input into the algae liquid by the air pump, and then after being absorbed and utilized by the algae liquid, the oxygen-rich air escapes from the algae liquid and is discharged from the air outlet of the tube cover.

[0059] With the increase of microalgae cells, the carbon fixation capacity and oxygen release of microalgae reached the highest level. By the end of the culture, the carbon fixation of Chlorella reached 2.57g / L, the carbon fixation of Porphyridium was 2.78g / L, and the carbon fixation of Spirulina was 1.59g / L. In terms of oxygen release, by the end of the culture, the oxygen release of Chlorella was 1.88g / L, the oxygen release of Porphyridium was 2.04g / L, and the oxygen release of Spirulina was 1.17g / L.

[0060] The above contents are intended to illustrate the technical means of this method, and are not intended to limit the technical scope of the present invention. Those skilled in the art may make obvious improvements or substitutions to the present invention in combination with existing common knowledge, which also fall within the scope of protection of the claims of the present invention.

Claims

1. A microalgae cultivation device coupled with air purification and fertilizer preparation, the microalgae cultivation device comprising a cylinder cover (10), a cylinder body (1) and a base (4), wherein at least one side of the cylinder body is transparent; characterized in that The cylinder (1) is divided into at least two culture chambers (101) along the circumferential direction. A transparent lamp chamber (102) for placing an auxiliary lamp (9) is provided in the middle of the cylinder. A liquid outlet (103) is provided at the bottom of each culture chamber of the cylinder. A liquid discharge valve (2) is provided at the liquid outlet. A sealed cover plate (5) and a fixing ring (7) are provided at the top of the cylinder. The fixing ring is threadedly fixed to the top of the cylinder and clamps the cover plate. A sealing gasket (501) is provided at the bottom of the cover plate for sealing and separating the top of each culture chamber in the cylinder from the top of the lamp chamber. An exhaust hole (502) and an air inlet pipe (503) communicating with each culture chamber are integrated on the surface of the cover plate. ), wherein the air inlet pipe extends into the bottom of the culture chamber, the bottom of the cylinder cover (10) and the top of the fixing ring are plug-in sealed, an air pump (8) is fixedly arranged inside the cylinder cover, the air inlet of the air pump is communicated with the outside of the cylinder cover, the air outlet of the air pump is branched and connected to each air inlet pipe of the cover plate, the cylinder cover is provided with an air outlet hole (1004) for guiding the gas discharged from the exhaust hole of the cover plate to the outside of the cylinder cover, and the cylinder cover is integrated with a light-controlled switch (1003) and an air pump switch (1002) for controlling the operation of the auxiliary light and the air pump; the bottom of the cylinder body is plug-in matched with the base (4), and a material receiving cavity (401) is formed inside the base.

2. The microalgae cultivation device for coupling air purification and fertilizer preparation according to claim 1, characterized in that The exhaust hole (502) of the cover plate (5) is provided with a thread and is threadedly connected to a one-way liquid discharge valve (6). The one-way liquid discharge valve comprises a valve body (601) and a valve core (602). The valve body is provided with a gas-liquid port (6011) communicating with the inside and outside of the cover plate. The inner end of the valve body is provided with a hollow bracket (6012) for limiting the position of the valve core. The valve core is limited in the hollow bracket. When the cylinder body is vertical, an exhaust gap is formed between the valve core and the gas-liquid port under the dead weight of the valve core, and the gas-liquid port is in an open state. When the valve core is pressed against the gas-liquid port by an external force, the gas-liquid port is closed.

3. The microalgae cultivation device for coupling air purification and fertilizer preparation according to claim 1, characterized in that The liquid discharge valve (2) of each liquid outlet (103) at the bottom of the cylinder (1) comprises a valve head (201), a valve seat, a spring (204), and a manual switch plate (3); the valve head is in an inverted cone shape and is limitedly located above the liquid outlet; the valve seat comprises a valve stem (202) and a valve plate (203) connected as one body; the top of the valve stem is threadedly connected to the valve head; the spring is sleeved on the valve stem and is limitedly located between the bottom surface of the cylinder and the valve plate; and under the action of the spring, the valve head and the liquid outlet are sealed; the manual switch plate is hinged to a hinge seat (105) integrated at the bottom of the cylinder; one end of the manual switch plate extends out of an opening (104) on the peripheral surface of the bottom of the cylinder, and the other end of the manual switch plate is located at the bottom of the valve seat; when the manual switch plate is pressed, the manual switch plate pushes the valve seat to release the sealing state between the valve head and the liquid outlet.

4. The microalgae cultivation device coupled with air purification and fertilizer preparation according to claim 1, characterized in that The volume of the material receiving cavity (401) in the base (4) is not less than the volume of one of the culture cavities (102) in the cylinder (1).

5. The microalgae cultivation device for coupling air purification and fertilizer preparation according to claim 1, characterized in that The base (4) is transparent.

6. The microalgae cultivation device coupled with air purification and fertilizer preparation according to claim 1, characterized in that The auxiliary lamp (9) is a color-changing LED lamp and is provided with remote control and timing control.

7. The microalgae cultivation device coupled with air purification and fertilizer preparation according to claim 1, characterized in that The lamp cavity (102) is convex relative to the culture cavity (101), and the convex portion positions the cover plate (5).

8. The microalgae cultivation device coupled with air purification and fertilizer preparation according to claim 1, characterized in that The side of the cylinder (1) is provided with scales for displaying the changes in the solutions in each culture chamber.

9. A method for using the microalgae cultivation device coupled with air purification and fertilizer preparation as claimed in claim 1, characterized in that The method of use is as follows: first, the cylinder (1) is plugged into the base (4), and the algae solution to be cultured is injected into the culture chamber (101) of the cylinder; then, the cover plate (5) is matched with the top of the cylinder, and the cover plate is threadedly connected to the top of the cylinder through a fixing ring (7) to lock the cover plate, so that the cover plate forms a sealed partition between the top of each culture chamber of the cylinder and the lamp chamber (102); next, the auxiliary lamp (9) connected to the cylinder cover (10) is inserted into the lamp chamber, the air outlet of the air pump (8) is sealed and connected to the air inlet pipe (503) of the cover plate through a branch joint (801) and a hose, and then the cylinder cover is plugged into the top of the fixing ring. The microalgae culture device is sealed, and the installation is completed; the microalgae culture device is placed in a lighted position or an auxiliary light is turned on, and an air pump is used to introduce external air into the bottom of the culture chamber, and the microalgae in the algae liquid purifies the air and converts carbon dioxide into oxygen through photosynthesis, and the oxygen is discharged to the outside through the exhaust hole (502) of the cover plate and the air outlet hole (1004) of the cylinder cover, thereby achieving air purification; after the microalgae culture is completed, the algae liquid is discharged into the material receiving chamber (401) of the base (4) through the liquid discharge valve (2) at the bottom of the cylinder body, and the base is removed, so that the algae liquid in the base can be used as fertilizer to directly fertilize the crops.

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