Cell counting instrument

Abstract

The invention provides a cell counting instrument, which comprises an illumination assembly used for illuminating a cell counting area and providing guarantee for a microscopic imaging assembly to obtain an image with uniform illumination; the chip sensing assembly is used for detecting position information and angle information of the cell counting plate; the microscopic imaging assembly comprises a lens, a tube lens, a camera, an adapter and one or more third reflectors, and is used for carrying out image acquisition on the cells in the cell counting chip; the cell counting instrument is designed into a module according to the high-throughput automatic detection requirement, the cell counting instrument is miniaturized and modularized through innovative optical, mechanical and electric control structures, and the size of the cell counting instrument is reduced through compact illumination microscopic imaging design, so that the cell counting instrument becomes a modularized component, and the cell counting instrument is convenient to use. And the device can be directly placed in a liquid work station for integration, so that the requirement of high-flux automatic detection can be well met.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical detection, and in particular to a cell counter. Background Art

[0002] In the field of biomedical testing, cell counting is a very important detection method and tool. It is used to perform statistical analysis on the number of human cells, various bacteria, fungi and even some specific particles, obtain the concentration, viability and other parameters of various cell suspensions, and provide researchers or clinicians with status information of the human body and biological reaction systems. Currently, there are two methods for cell counting: manual counting and instrument-automated counting. The counting process and steps are the same, and the difference is the final counting method. Manual counting uses the naked eye to count cells, while instrument-automated counting uses computers to collect images and process them to obtain results.

[0003] High-throughput automated testing is the development trend and urgent need of all walks of life, especially medical testing. Liquid pipetting workstations are combined with various detection modules to achieve everything from sample pre-treatment to result output, which is the core means of high-throughput automation. Taking into account the core requirements such as instrument integration, pipetting arm span and lifting space, miniaturization and time efficiency, high-throughput automated testing usually requires modularization and miniaturization of conventional instruments to meet the needs of detection and integration. However, existing automatic cell counting instruments have the problems of large size, cumbersome detection process, and inability to integrate with liquid pipetting workstations, which hinders the development of cell counting technology and high-throughput automated testing, and is not conducive to scientific experimental research and clinical testing. For this reason, a cell counter is proposed. Summary of the invention

[0004] In view of this, the embodiments of the present invention hope to provide a cell counter to solve or alleviate the technical problems existing in the prior art and at least provide a beneficial choice.

[0005] The technical solution of the embodiment of the present invention is implemented as follows: a cell counter, comprising:

[0006] The lighting component is used to illuminate the cell counting area and provide a guarantee for the microscopic imaging component to obtain an image with uniform illumination;

[0007] Chip sensing component, used to detect the position and angle information of the cell counting plate;

[0008] A microscopic imaging assembly, including a lens, a tube lens, a camera, an adapter, and one or more third reflectors, for capturing images of cells in a cell counting chip;

[0009] An autofocus component, used to drive the lens to move closer to or farther from the cell counting chip;

[0010] A multi-area scanning component is used to drive the lens and the autofocus component to move in a direction parallel to the cell counting plate;

[0011] The central control component is used to control image acquisition, autofocus, and human-computer interaction equipment.

[0012] In some embodiments, the lighting assembly includes a light source, a first reflector, a light source heat dissipation component, a plurality of focusing units, a plurality of focusing element mounting components, a second reflector, an aperture, a first reflector fixing component, a second reflector fixing component, a homogenizing device, a plurality of fixing components and a guide rail, wherein:

[0013] The light source is arranged on the second reflector fixing part, and the light source heat dissipation part is arranged on the second reflector fixing part, and the light source heat dissipation part is used to dissipate heat from the light source;

[0014] If the first reflector is evenly arranged on the second reflector fixing part, and the homogenizing device is arranged on the second reflector fixing part;

[0015] A plurality of the focusing units are respectively arranged in a plurality of the focusing element mounting components, and a plurality of the fixing elements are evenly arranged on the corresponding focusing units and the corresponding focusing element mounting components;

[0016] The outer walls of the plurality of focusing element mounting components are each provided with a sliding groove, and the guide rail slides on the inner wall of the sliding groove;

[0017] The second reflector is evenly arranged on the first reflector fixing part, and the aperture is arranged on the first reflector fixing part.

[0018] In some embodiments, the chip sensing component includes one or more distance sensors for detecting the cell counting chip, and the distance sensors are arranged on the microscopic imaging component.

[0019] In some embodiments, the cell counting chip is located in front of the lens, the tube lens is located behind the lens, the tube lens is located in front of the camera, the adapter is located in front of the camera, the tube lens is located in front of the adapter, one or more third reflectors are located between the tube lens, the lens and the camera, and the lens is disposed on the autofocus assembly.

[0020] In some embodiments, the autofocus assembly includes a first mounting seat, a first power mechanism, a second mounting seat; a third mounting seat, a fourth mounting seat, a first transmission mechanism, a first guide mechanism and a first position feedback mechanism, wherein:

[0021] The first power mechanism is disposed on the first mounting seat, and the third mounting seat is disposed on the lower surface of the second mounting seat;

[0022] The lens is arranged on the outer wall of the second mounting seat, and the fourth mounting seat is adapted to the third mounting seat;

[0023] The first guide mechanism is arranged on a side of the fourth mounting seat adjacent to the third mounting seat;

[0024] The first transmission mechanism is arranged on the first guide mechanism, and the first power mechanism is arranged on the first transmission mechanism;

[0025] The first position feedback mechanism is disposed on the outer wall of the fourth mounting seat;

[0026] The multi-region scanning component is arranged on the lower surface of the fourth mounting seat.

[0027] In some embodiments, the multi-region scanning assembly includes a fifth mounting seat, a second power mechanism, a sixth mounting seat, a seventh mounting seat, a second transmission mechanism, a second guide mechanism, and a second position feedback mechanism, wherein:

[0028] The second power mechanism is arranged on the fifth mounting seat, and the second power mechanism is arranged on the second transmission mechanism;

[0029] The sixth mounting seat is matched with the seventh mounting seat, and the second guide mechanism is arranged on a side of the sixth mounting seat adjacent to the seventh mounting seat;

[0030] The second position feedback mechanism is disposed on the outer wall of the seventh mounting seat, and the second position feedback mechanism has the same structure as the first position feedback mechanism;

[0031] The sixth mounting seat is arranged on the lower surface of the fourth mounting seat.

[0032] In some embodiments, the first position feedback mechanism includes a plurality of single-point position sensors and rotation angle sensors, wherein:

[0033] The rotation angle sensor is arranged on the first power mechanism;

[0034] A plurality of the single-point position sensors are evenly arranged on the outer wall of the fourth mounting seat.

[0035] In some embodiments, the first position feedback mechanism further includes a continuous displacement sensor, wherein:

[0036] The continuous displacement sensor is arranged on the outer wall of the third mounting seat.

[0037] In some embodiments, the central control component includes an image acquisition and data processing module, an autofocus component control module, a chip sensing component control module, a data communication module, a human-computer interaction module and a multi-region scanning component control module, wherein:

[0038] The image acquisition and data processing module is electrically connected to the chip sensing component and the microscopic imaging component through wires;

[0039] The auto-focus component control module is electrically connected to the auto-focus component via an electric wire;

[0040] The chip sensing component control module is electrically connected to the chip sensing component through wires;

[0041] The multi-region scanning component control module is electrically connected to the multi-region scanning component through wires;

[0042] The data communication module is electrically connected to the image acquisition and data processing module, the autofocus component control module, the chip sensing component control module, the human-computer interaction module and the multi-region scanning component control module through wires.

[0043] A cell counting method comprises the following steps:

[0044] Step 1: Place the cell counting chip at the approximate working distance in front of the cell counting module lens;

[0045] Step 2: The chip sensing component automatically detects and obtains the position and posture information of the cell counting chip;

[0046] Step 3: The image acquisition and data processing module automatically acquires cell images and calculates the clarity and sharpness of the cell images;

[0047] Step 4: The auto-focus component control module automatically calculates the direction and displacement of the next movement of the auto-focus component according to the clarity and sharpness of the cell image;

[0048] Step 5: The image acquisition and data processing module automatically acquires cell images, calculates the clarity and sharpness of the cell images, and determines whether the optimal clarity has been achieved. If not, it is adjusted to step 5;

[0049] Step 6: The image acquisition and data processing module acquires cell images and calculates cell concentration and viability;

[0050] Step 7: The multi-region scanning component drives the autofocus component to perform multi-region scanning, and at the same time compensates the focus position during the multi-region scanning component according to the cell counting chip position and posture information obtained by the chip sensing component;

[0051] Step 8: Complete multi-area scanning and calculate cell concentration and viability.

[0052] The embodiment of the present invention has the following advantages due to the adoption of the above technical solution:

[0053] Aiming at the demand of high-throughput automated detection, the present invention designs the cell counting instrument into a module, and miniaturizes and modularizes it through innovative optical, mechanical and electrical control structures. Through the compact illumination microscopic imaging design, the volume of the cell counter is reduced, making it a modular component that can be directly placed in a liquid workstation for integration, thereby meeting the demand of high-throughput automated detection.

[0054] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present invention will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or technical descriptions will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0056] Figure 1 It is a structural diagram of the present invention;

[0057] Figure 2 is a structural diagram of the lighting assembly of the present invention;

[0058] Figure 3 It is a structural diagram of the illumination light path of the present invention;

[0059] Figure 4 is a structural diagram of a lighting assembly having a first reflector according to the present invention;

[0060] Figure 5 is a structural diagram of a lighting assembly having a first reflector and a second reflector according to the present invention;

[0061] Figure 6 A partial structural diagram of the lighting assembly of the present invention;

[0062] Figure 7 This is a structural diagram of the chip sensing component of the present invention;

[0063] Figure 8 is a structural diagram of the imaging assembly of the present invention;

[0064] Fig. 9 It is a structural diagram of an imaging assembly with an adapter according to the present invention;

[0065] Fig.10 It is a structural diagram of an imaging assembly having a third reflector according to the present invention;

[0066] Fig.11 is a structural diagram of an imaging assembly having two third reflectors according to the present invention;

[0067] Fig.12 is a structural diagram of an imaging assembly having four third reflectors according to the present invention;

[0068] Fig.13 is a structural diagram of the automatic focusing assembly of the present invention;

[0069] Fig.14 It is a structural diagram of the automatic focusing assembly of the present invention from a side view;

[0070] Fig.15 This is a schematic diagram of multi-region scanning of the present invention;

[0071] Fig.16 is a structural diagram of a multi-region scanning assembly of the present invention;

[0072] Fig.17 It is a structural diagram of a multi-region scanning assembly from a side view of the present invention;

[0073] Fig.18 Schematic diagram of the central control component of the present invention Figure 1 ;

[0074] Fig.19 Schematic diagram of the central control component of the present invention Figure 2 ;

[0075] Fig. 20 It is a structural diagram of the installation of the rotation angle sensor and the single point position sensor of the present invention;

[0076] Fig.21 It is a structural diagram of the installation of the continuous displacement sensor of the present invention;

[0077] Fig. 22 It is a front view structural diagram of the automatic focusing assembly of the present invention;

[0078] Fig.23 It is a structural diagram of a cell counter having a first reflector and a third reflector according to the present invention;

[0079] Fig.24 It is a structural diagram of the simple cell counter of the present invention.

[0080] Figure numerals: 1, lighting component; 2, chip sensing component; 3, microscopic imaging component; 5, autofocus component; 6, multi-area scanning component; 7, central control component; 8, cell counting chip; 101, light source; 102, first reflector; 103, light source heat dissipation component; 104, focusing unit; 105, focusing element mounting component; 108, second reflector; 109, aperture; 110, first reflector fixing component; 111, second reflector fixing component; 112, homogenizing device; 113, fixing component; 114, sliding groove; 115, guide rail; 201, distance sensor; 301, lens; 302, tube mirror; 303, camera; 304, adapter; 305, third reflector; 501, first mounting seat; 502, first power mechanism ; 503, second mounting seat; 504, third mounting seat; 505, fourth mounting seat; 506, first transmission mechanism; 507, first guiding mechanism; 508, first position feedback mechanism; 601, fifth mounting seat; 602, second power mechanism; 604, sixth mounting seat; 605, seventh mounting seat; 606, second transmission mechanism; 607, second guiding mechanism; 608, second position feedback mechanism; 701, image acquisition and data processing module; 702, autofocus component control module; 703, chip sensing component control module; 705, data communication module; 706, human-computer interaction module; 707, multi-area scanning component control module; 5081, single-point position sensor; 5082, rotation angle sensor; 5083, continuous displacement sensor. DETAILED DESCRIPTION

[0081] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0082] It should be noted that the terms "first", "second", "symmetrical", "array", etc. are only used to distinguish between description and position description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the definition of "first", "symmetrical", etc. can explicitly or implicitly include one or more of these features; similarly, when the quantity of certain features is not limited in the form of words such as "two" or "three", it should be noted that this feature also explicitly or implicitly includes one or more feature quantities;

[0083] In the present invention, unless otherwise clearly specified and limited, the terms such as "installation", "connection", "fixation" and the like should be understood in a broad sense; for example, it can be a fixed connection, a detachable connection, or an integral molding; it can be a mechanical connection, a direct connection, welding, or an indirect connection through an intermediate medium, or it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood based on the description and drawings combined with specific circumstances.

[0084] The embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0085] Embodiment 1

[0086] like Figure 1-Figure 24 As shown, an embodiment of the present invention provides a cell counter, comprising:

[0087] The lighting component 1 is used to illuminate the cell counting area, so as to ensure that the microscopic imaging component 3 obtains an image with uniform illumination;

[0088] Chip sensing component 2, used to detect the position information and angle information of the cell counting plate;

[0089] The microscopic imaging assembly 3 includes a lens 301, a tube lens 302, a camera 303, an adapter 304 and one or more third reflectors 305, and is used to collect images of cells in the cell counting chip 8;

[0090] The auto-focus component 5 is used to drive the lens 301 to move closer to or farther from the cell counting chip 8;

[0091] The multi-area scanning component 6 is used to drive the lens 301 and the autofocus component 5 to move in a direction parallel to the cell counting plate;

[0092] The central control component 7 is used to control image acquisition, autofocus, and human-computer interaction equipment.

[0093] In this embodiment, specifically, the lighting assembly 1 includes a light source 101, a first reflector 102, a light source heat dissipation component 103, a plurality of focusing units 104, a plurality of focusing element mounting components 105, a second reflector 108, an aperture 109, a first reflector fixing component 110, a second reflector fixing component 111, a homogenizing device 112, a plurality of fixing components 113 and a guide rail 115, wherein:

[0094] The light source 101 is disposed on the second reflector fixing part 111, and the light source heat dissipation part 103 is disposed on the second reflector fixing part 111, and the light source heat dissipation part 103 is used to dissipate heat for the light source 101;

[0095] If the first reflector 102 is evenly disposed on the second reflector fixing part 111 , and the homogenizing device 112 is disposed on the second reflector fixing part 111 ;

[0096] A plurality of focusing units 104 are respectively disposed in a plurality of focusing element mounting components 105, and a plurality of fixing elements 113 are evenly disposed on corresponding focusing units 104 and corresponding focusing element mounting components 105;

[0097] The outer walls of the plurality of focusing element mounting components 105 are each provided with a sliding groove 114, and the guide rail 115 slides on the inner wall of the sliding groove 114;

[0098] The second reflector 108 is evenly disposed on the first reflector fixing part 110 , and the aperture 109 is disposed on the first reflector fixing part 110 .

[0099] In this embodiment, specifically, the light source 101 is a device used to provide sufficiently strong and stable illumination for cell imaging. The light source 101 includes but is not limited to LED lamp beads, halogen lamps or other light sources 101 that can emit stable and sufficiently strong illumination. Preferably, the light source 101 is an LED lamp bead, and the light source 101 can emit a single or continuous spectrum.

[0100] The focusing unit 104 is used to converge the light emitted by the light source 101 onto the sample to obtain sufficient illumination for use by the microscope imaging component 3. The focusing unit 104 includes but is not limited to a lens. The focusing unit 104 can be one or more. The beneficial effect of using multiple focusing units 104 is that more uniform beam shaping and illumination can be obtained. The focusing unit 104 is used to image the homogenized light source image onto the sample surface. This process is called critical illumination.

[0101] In this embodiment, specifically, the light homogenizer 112 is used to homogenize the non-uniform light source 101 to eliminate the non-uniformity of the light source 101 itself. The light homogenizer 112 includes but is not limited to a flyback lens, frosted glass, a light homogenizer rod, and a beam shaping lens.

[0102] In this embodiment, specifically, the aperture 109 is used to form a window in the light path to prevent light outside the aperture 109 from entering the illumination area. The beneficial effect of using the aperture 109 is that the contrast of microscopic imaging can be improved.

[0103] One or more first reflectors 102 are used to reflect light back, which has the beneficial effect of reducing the size of the device.

[0104] In this embodiment, specifically, Figure 3In the figure, the cell counting chip 8 is located at a working distance in front of the lens 301. The light emitted by the light source 101 is homogenized by the light homogenizer 112 and has a certain divergence angle. The focusing unit 104 converges the light and images the light source onto the cell counting chip 8 to obtain a uniform light spot. The aperture 109 is used to limit the illumination beam to improve the illumination quality and imaging contrast.

[0105] In this embodiment, specifically, the aperture 109 is located between the focusing unit 104 and the cell counting chip 8 .

[0106] In this embodiment, specifically, the aperture 109 is located between the focusing unit 104 and the light homogenizing element 112 .

[0107] In this embodiment, specifically, Figure 4 In the figure, an illumination light path with two focusing units 104 is shown. When two or more focusing units 104 are used, the shaping of the light path will be softer, so it is easier to obtain a light spot with good uniformity, and Kohler illumination can be constructed. Kohler illumination is an illumination method with high light source utilization and high uniformity, and its light spot quality is not affected by the light source.

[0108] In this embodiment, specifically, the first reflector 102 and the second reflector 108 include but are not limited to a plane reflector and a reflective prism. The first reflector 102 and the second reflector 108 function to fold back the light path, thereby reducing the volume of the illumination light path.

[0109] Figure 4 It is used to illustrate an illumination light path with a first reflector 102. The first reflector 102 reflects the homogenized light by 90 degrees, and the light source 101 and the homogenizing device 112 are placed on one side, thereby reducing the total length of the light path.

[0110] Figure 5 The illumination light path of the first reflector 102 and the second reflector 108 is shown. The turning of the light path by the first reflector 102 and the second reflector 108 further reduces the total length of the light path, providing a basis for miniaturized design.

[0111] In this embodiment, specifically, Figure 6 In the figure, the focusing element mounting component 105 has a structural sliding groove 114 that cooperates with the guide rail 115. The focusing element mounting component 105 can be adjusted in the direction of the guide rail 115 under the constraint of the structural sliding groove 114, thereby driving the movement of the focusing unit 104 on the optical path to optimize the optical path shaping and illumination.

[0112] After the optical path adjustment and optimization is completed, the focusing element mounting component 105 is fastened to the guide rail 115 through the fixing element 113. Preferably, the fixing element 113 is a top screw or a screw.

[0113] In this embodiment, specifically, the chip sensing component 2 includes one or more distance sensors 201 for detecting the cell counting chip 8 , and the distance sensor 201 is disposed on the microscopic imaging component 3 .

[0114] In this embodiment, specifically, the distance sensor 201 can measure the distance information between the cell counting chip 8 and the distance sensor 201. The distance sensor 201 includes but is not limited to an inductive position sensor, a diffuse reflection photoelectric switch, a laser distance sensor, and the like.

[0115] In this embodiment, specifically, Figure 7 In the figure, a device including a plurality of distance sensors 201 is shown. When one distance sensor 201 is included, information on the presence or absence of a cell counting chip 8 and the distance of the cell counting chip 8 can be obtained.

[0116] In this embodiment, specifically, Figure 7 In the figure, a device including a plurality of distance sensors 201 is shown. When two distance sensors 201 are included, not only the presence or absence of a cell counting chip 8 and the distance between the cell counting chip 8 and the distance sensor 201 can be obtained, but also the posture information of the cell counting chip 8 in one dimension can be obtained.

[0117] In this embodiment, specifically, two displacement sensors 201 are used to detect the information of points B and C, and the posture information of the cell counting chip 8 rotating along the y-axis can be obtained. Similarly, when detecting points A and B, the posture information of the cell counting chip 8 rotating along the x-axis can be obtained.

[0118] In this embodiment, specifically, when three or more distance sensors 201 are used, the posture information of the cell counting chip 8 in the x and y directions can be obtained simultaneously, and the distance information at the center of the cell counting chip 8 can be obtained simultaneously.

[0119] In this embodiment, specifically, the cell counting chip 8 is located in front of the lens 301, the tube lens 302 is located behind the lens 301, the tube lens 302 is located in front of the camera 303, the adapter 304 is located in front of the camera 303, the tube lens 302 is located in front of the adapter 304, one or more third reflectors 305 are located between the tube lens 302, the lens 301 and the camera 303, and the lens 301 is set on the autofocus component 5.

[0120] In this embodiment, specifically, the lens 301 is used to optically magnify the cell sample, and its magnification includes but is not limited to 2.5×, 4×, 5×, 10×, 20×, and 40×. Preferably, the magnification of the lens 301 is 10×.

[0121] The lens 301 is composed of a single lens or a group of lenses. The lens 301 includes but is not limited to an objective lens. The objective lens includes but is not limited to a finite element plan achromatic objective lens and an infinity plan achromatic objective lens. Preferably, the objective lens is an infinity plan achromatic objective lens.

[0122] In this embodiment, specifically, when the lens 301 uses an infinite objective lens, the tube lens 302 is used to image the image in the camera. Preferably, the tube lens 302 is an infinite achromatic correction lens, and the focal length of the tube lens 302 includes but is not limited to f=180mm and f=200mm.

[0123] The camera 303 is used to collect image information of the cell sample formed by the lens 301 and the tube lens 302, including but not limited to a CCD camera and a CMOS camera.

[0124] The adapter 304 is located at the front end of the camera 303. The adapter 304 is composed of one or more lenses and can enlarge or reduce the imaging field of view.

[0125] In this embodiment, specifically, Fig.10 In the embodiment, the third reflector 305 forms a 90° turn on the optical path, which has the beneficial effect of reducing the length of the imaging optical path and reducing the volume.

[0126] In this embodiment, specifically, Fig.11 In FIG. 1 , an imaging assembly including two third reflectors 305 is shown. The length of the imaging optical path is further reduced by folding back the reflectors 305 twice.

[0127] In this embodiment, specifically, Fig.12 Four third reflectors 305 are used to reflect the light path four times.

[0128] In this embodiment, specifically, the autofocus assembly 5 includes a first mounting seat 501, a first power mechanism 502, a second mounting seat 503; a third mounting seat 504, a fourth mounting seat 505, a first transmission mechanism 506, a first guide mechanism 507 and a first position feedback mechanism 508, wherein:

[0129] The first power mechanism 502 is disposed on the first mounting seat 501 , and the third mounting seat 504 is disposed on the lower surface of the second mounting seat 503 ;

[0130] The lens 301 is disposed on the outer wall of the second mounting seat 503, and the fourth mounting seat 505 is adapted to the third mounting seat 504;

[0131] The first guide mechanism 507 is disposed on one side of the fourth mounting seat 505 adjacent to the third mounting seat 504;

[0132] The first transmission mechanism 506 is disposed on the first guide mechanism 507, and the first power mechanism 502 is disposed on the first transmission mechanism 506;

[0133] The first position feedback mechanism 508 is disposed on the outer wall of the fourth mounting seat 505;

[0134] The multi-region scanning assembly 6 is disposed on the lower surface of the fourth mounting base 505 .

[0135] In this embodiment, specifically, the autofocus assembly 5 is used to drive the lens 301 to move toward or away from the cell counting plate.

[0136] The first power mechanism 502 is used to generate rotational or linear motion power.

[0137] The first power mechanism 502 includes but is not limited to a stepper motor, a DC motor, an AC motor, a linear motor and a voice coil motor. Preferably, the first power mechanism 502 is a stepper motor.

[0138] The first transmission mechanism 506 includes but is not limited to a screw-nut, a synchronous belt, and a gear rack.

[0139] The first guide mechanism 507 includes but is not limited to a guide rail.

[0140] In this embodiment, specifically, Fig.12 In the embodiment, the autofocus assembly 5 is used to drive the lens 301 to move toward or away from the cell counting plate 8.

[0141] When a finite element optical system is used, the autofocus component 5 needs to drive the entire microscopic imaging component 3 to move toward or away from the cell counting plate 8 .

[0142] When an infinite optical system is used, the autofocus assembly 5 only needs to drive the lens 301 to move closer to or farther from the cell counting plate 8 .

[0143] In this embodiment, specifically, the multi-region scanning assembly 6 includes a fifth mounting seat 601, a second power mechanism 602, a sixth mounting seat 604, a seventh mounting seat 605, a second transmission mechanism 606, a second guide mechanism 607 and a second position feedback mechanism 608, wherein:

[0144] The second power mechanism 602 is disposed on the fifth mounting seat 601, and the second power mechanism 602 is disposed on the second transmission mechanism 606;

[0145] The sixth mounting seat 604 is matched with the seventh mounting seat 605, and the second guide mechanism 607 is disposed on a side adjacent to the sixth mounting seat 604 and the seventh mounting seat 605;

[0146] The second position feedback mechanism 608 is disposed on the outer wall of the seventh mounting seat 605 , and the second position feedback mechanism 608 has the same structure as the first position feedback mechanism 508 ;

[0147] The sixth mounting seat 604 is disposed on the lower surface of the fourth mounting seat 505 .

[0148] In this embodiment, specifically, when a finite element optical system is used, the multi-region scanning component 6 needs to drive the entire microscopic imaging component 3 and the autofocus component 5 to move toward or away from the cell counting plate 8 .

[0149] In this embodiment, specifically, when an infinite optical system is used, the multi-region scanning component 6 only needs to drive the autofocus component 5 as well as the lens 301 and the third reflector 305 to move parallel to the cell counting plate 8 .

[0150] In this embodiment, specifically, the second power mechanism 602 is used to generate power for rotation or linear motion. The second power mechanism 602 includes but is not limited to a stepper motor, a DC motor, an AC motor and a linear motor. Preferably, the second power mechanism 602 is a stepper motor.

[0151] The second transmission mechanism 606 includes but is not limited to a screw-nut.

[0152] The second guiding mechanism 607 includes but is not limited to a guide rail.

[0153] In this embodiment, specifically, the first position feedback mechanism 508 includes a plurality of single-point position sensors 5081 and a rotation angle sensor 5082 , wherein the rotation angle sensor 5082 is arranged on the first power mechanism 502 , and the plurality of single-point position sensors 5081 are evenly arranged on the outer wall of the fourth mounting seat 505 .

[0154] In this embodiment, specifically, the first position feedback mechanism 508 further includes a continuous displacement sensor 5083 , wherein the continuous displacement sensor 5083 is disposed on an outer wall of the third mounting seat 504 .

[0155] In this embodiment, specifically, the single-point position sensor 5081 includes but is not limited to a reflected photoelectric switch, a reflected photoelectric switch, a contact switch, etc., and is used for zero position detection of the autofocus component 5.

[0156] The rotation angle sensor 5082 includes, but is not limited to, a rotary encoder.

[0157] The continuous displacement sensor 5083 includes but is not limited to a linear grating encoder and an inductive position sensor, which is used to obtain the angle information of the rotating motor in real time and calculate the real-time position of the autofocus component 5 according to the lead of the transmission screw.

[0158] In this embodiment, specifically, the central control component 7 includes an image acquisition and data processing module 701, an autofocus component control module 702, a chip sensing component control module 703, a data communication module 705, a human-computer interaction module 706 and a multi-region scanning component control module 707, wherein:

[0159] The image acquisition and data processing module 701 is electrically connected to the chip sensing component 2 and the microscopic imaging component 3 through wires;

[0160] The auto-focus component control module 702 is electrically connected to the auto-focus component 5 via wires;

[0161] The chip sensing component control module 703 is electrically connected to the chip sensing component 2 through wires;

[0162] The multi-region scanning component control module 707 is electrically connected to the multi-region scanning component 6 through wires;

[0163] The data communication module 705 is electrically connected to the image acquisition and data processing module 701, the auto-focus component control module 702, the chip sensing component control module 703, the human-computer interaction module 706 and the multi-region scanning component control module 707 through wires.

[0164] In this embodiment, specifically, the image acquisition and data processing module 701 includes but is not limited to functions such as image acquisition, cell recognition and viability statistics, and image clarity and sharpness calculation.

[0165] The chip sensing component control module 703 is used to receive the signal detected by the chip sensing component 2, and calculate the existence, position and posture information of the cell counting chip 8 according to the signal, for use by the central control component 7, especially the autofocus component control module 702.

[0166] The data communication module 705 includes one or more communication buses for data interaction with other external devices. The communication buses include wired networks and wireless networks. The communication buses include but are not limited to RS232, RS485, CAN, USB, WI FI, Bluetooth and Ethernet interfaces.

[0167] The human-computer interaction module 706 is used for interaction between the module and humans, and its components include but are not limited to buttons, displays, scanners, and voice devices.

[0168] The auto-focus component control module 702 controls the auto-focus component 5 to complete the auto-focusing work through the image clarity and sharpness obtained by the image acquisition and data processing module.

[0169] The control module of the auto-focus component 5 controls the auto-focus component 5 to complete the auto-focusing work through the chip position and posture information obtained by the chip sensing component 2.

[0170] The multi-region scanning component control module 707 controls the multi-region scanning component to complete scanning and imaging of multiple cell imaging regions.

[0171] A cell counting method comprises the following steps:

[0172] Step 1: Place the cell counting chip 8 at the approximate working distance of the front end of the cell counting module lens 301;

[0173] Step 2: The chip sensing component 2 automatically detects and obtains the position and posture information of the cell counting chip 8;

[0174] Step 3: The image acquisition and data processing module 701 automatically acquires cell images and calculates the clarity and sharpness of the cell images;

[0175] Step 4: The auto-focus component control module 702 automatically calculates the direction and displacement of the next movement of the auto-focus component 5 according to the clarity and sharpness of the cell image;

[0176] Step 5: The image acquisition and data processing module 701 automatically acquires cell images, calculates the clarity and sharpness of the cell images, and determines whether the optimal clarity has been achieved. If not, the process proceeds to step 4.

[0177] Step 6: The image acquisition and data processing module 701 acquires cell images and calculates cell concentration and viability;

[0178] Step 7: The multi-region scanning component 6 drives the auto-focus component 5 to perform multi-region scanning, and at the same time, according to the position and posture information of the cell counting chip 8 obtained by the chip sensing component 2, compensates the focus position of the multi-region scanning component 6 during the process;

[0179] Step 8: Complete multi-area scanning and calculate cell concentration and viability.

[0180] Embodiment 2

[0181] A miniature modular cell counter, comprising an illumination component 1, a chip sensing component 2, a microscopic imaging component 3, a central control component 7, an autofocus component 5, a multi-region scanning component 6 and a central control component 7, see Figure 1 ;

[0182] The lighting assembly 1 includes a light source 101, a light homogenizing device 112, a first reflector 102, two focusing units 104, a second reflector 108 and an aperture 109. Figure 5 Among them, the light source 101 is a high-power LED lamp bead with a power of 3W and a voltage of 3.7-5V.

[0183] The light homogenizing device 112 is a complex eye lens with a focal length of 25 mm.

[0184] The two focusing units 104 are lenses with focal lengths of 15 mm and 30 mm respectively.

[0185] The first reflector 102 and the second reflector 108 are right-angle reflective prisms, and the surfaces thereof are coated with AG total reflection films.

[0186] The aperture of the aperture 109 is 10 mm. Figure 2 and Figure 5 .

[0187] Chip sensing component 2 is four laser ranging sensors, see Figure 7 , the position and posture information of the cell counting chip 8 can be obtained.

[0188] The microscope imaging assembly 3 includes a lens 301, four third reflecting prisms 305, a tube lens 302 and a camera 303, wherein the lens 301 is an infinite plan achromatic objective lens with 10 times magnification, and the tube lens 302 is an achromatic infinite correction lens with a focal length of f=180 mm.

[0189] The third reflecting prism 305 is a right-angle reflecting prism, and the surface of the third reflecting prism is coated with an AG total reflection film.

[0190] The camera 303 is a 6-megapixel CCD camera with a pixel size of 2.0 μm×2.0 μm.

[0191] The autofocus component 5 is a linear module, which includes a first power mechanism 502, a first transmission mechanism 506, a first guide mechanism 507, and a first position feedback mechanism 508. The first power mechanism 502 is a 28 stepper motor, and the first transmission mechanism 506 is a screw nut structure with a lead of 2 mm.

[0192] The first guide mechanism 506 is a ball screw structure, and the first position feedback mechanism 508 includes three position pairs of single-point position sensors 5081 and rotation angle sensors 5082. The single-point position sensors 5081 are used as the travel switch and zero point information of the slide, and the rotary encoder is used as the position feedback of the slide. The entire slide is 6.5mm in diameter, and the displacement repeatability is 5μm. Fig. 20 and Fig. 22 .

[0193] The multi-region scanning component 6 is similar to the auto-focus component 5 and is a linear module.

[0194] The central control component 7 includes an image acquisition and data processing module 701, an autofocus component control module 702, a multi-area scanning component control module 707, a chip sensing component control module 703, a data communication module 705 and a human-computer interaction module 706, etc. Fig.18 .

[0195] The image acquisition and data processing module 701 is responsible for functions such as image acquisition, cell identification and viability statistics, and image clarity and sharpness calculation.

[0196] The chip sensing component control module 703 is used to receive the signal detected by the chip sensing component, and calculate the existence, position and posture information of the cell counting chip 8 according to the signal, for use by the image acquisition and data processing module 701, the autofocus component control module 702 and the multi-region scanning component control module 707.

[0197] The auto-focus component control module 702 drives the movement direction of the auto-focus component 5 according to the sharpness and clarity of the collected image. More precisely, it drives the auto-focus component 5 to drive the lens 301 to move closer to or away from the cell counting chip 8 according to the change gradient of the sharpness and clarity of the collected image.

[0198] The multi-region scanning component control module 707 is used for the multi-region scanning component 6 to drive the autofocus component 5 to move in a direction parallel to the cell counting chip 8, and to collect cell images during the movement. At the same time, the multi-region scanning component control module 707 also includes a calculation for compensating the focus position during the scanning process according to the position and posture of the cell counting chip 8 obtained by the chip sensing component control module 703.

[0199] A cell counting method comprises the following steps:

[0200] Step 1: Place the cell counting chip 8 at the approximate working distance of the front end of the cell counting module lens 301;

[0201] Step 2: The chip sensing component 2 automatically detects and obtains the position and posture information of the cell counting chip 8;

[0202] Step 3: The image acquisition and data processing module 701 automatically acquires cell images and calculates the clarity and sharpness of the cell images;

[0203] Step 4: The auto-focus component control module 702 automatically calculates the direction and displacement of the next movement of the auto-focus component 5 according to the clarity and sharpness of the cell image;

[0204] Step 5: The image acquisition and data processing module 701 automatically acquires cell images, calculates the clarity and sharpness of the cell images, and determines whether the optimal clarity has been achieved. If not, the process proceeds to step 4.

[0205] Step 6: The image acquisition and data processing module 701 acquires cell images and calculates cell concentration and viability;

[0206] Step 7: The multi-region scanning component 6 drives the auto-focus component 5 to perform multi-region scanning, and at the same time, according to the position and posture information of the cell counting chip 8 obtained by the chip sensing component 2, compensates the focus position of the multi-region scanning component 6 during the process;

[0207] Step 8: Complete multi-area scanning and calculate cell concentration and viability.

[0208] Embodiment 3

[0209] A miniature modular cell counter, comprising an illumination component 1, a chip sensing component 2, a microscopic imaging component 3, a central control component 7, an autofocus component 5, a multi-region scanning component 6 and a central control component 7, see Figure 1 ;

[0210] The lighting assembly 1 includes a light source 101, a light homogenizing device 112, a first reflector 102, two focusing units 104, a second reflector 108 and an aperture 109. Figure 5 Among them, the light source 101 is a high-power LED lamp bead with a power of 3W and a voltage of 3.7-5V.

[0211] The light homogenizing device 112 is a complex eye lens with a focal length of 25 mm.

[0212] The two focusing units 104 are lenses with focal lengths of 15 mm and 30 mm respectively.

[0213] The first reflector 102 and the second reflector 108 are right-angle reflective prisms, and the surfaces thereof are coated with AG total reflection films.

[0214] The aperture of the aperture 109 is 10 mm. Figure 2 and Figure 5 .

[0215] Chip sensing component 2 is four laser ranging sensors, see Figure 7 , the position and posture information of the cell counting chip 8 can be obtained.

[0216] The microscope imaging assembly 3 includes a lens 301, four third reflecting prisms 305, a tube lens 302 and a camera 303, wherein the lens 301 is an infinite plan achromatic objective lens with 10 times magnification, and the tube lens 302 is an achromatic infinite correction lens with a focal length of f=180 mm.

[0217] The third reflecting prism 305 is a right-angle reflecting prism, and the surface of the third reflecting prism is coated with an AG total reflection film.

[0218] The camera 303 is a 6-megapixel CCD camera with a pixel size of 2.0 μm×2.0 μm.

[0219] The autofocus component 5 is a linear module, which includes a first power mechanism 502, a first transmission mechanism 506, a first guide mechanism 507, and a first position feedback mechanism 508. The first power mechanism 502 is a 28 stepper motor, and the first transmission mechanism 506 is a screw nut structure with a lead of 2 mm.

[0220] The first guide mechanism 506 is a ball screw structure, and the first position feedback mechanism 508 includes three position pairs of single-point position sensors 5081 and rotation angle sensors 5082. The single-point position sensors 5081 are used as the travel switch and zero point information of the slide, and the rotary encoder is used as the position feedback of the slide. The entire slide is 6.5mm in diameter, and the displacement repeatability is 5μm. Fig. 20 and Fig. 22 .

[0221] The multi-region scanning component 6 is similar to the auto-focus component 5 and is a linear module.

[0222] The central control component 7 includes an image acquisition and data processing module 701, an autofocus component control module 702, a multi-area scanning component control module 707, a chip sensing component control module 703, a data communication module 705 and a human-computer interaction module 706, etc. Fig.18 .

[0223] The image acquisition and data processing module 701 is responsible for functions such as image acquisition, cell identification and viability statistics, and image clarity and sharpness calculation.

[0224] The chip sensing component control module 703 is used to receive the signal detected by the chip sensing component, and calculate the existence, position and posture information of the cell counting chip 8 according to the signal, for use by the image acquisition and data processing module 701, the autofocus component control module 702 and the multi-region scanning component control module 707.

[0225] The auto-focus component control module 702 drives the movement direction and displacement of the auto-focus component 5 according to the position and posture signal of the cell counting chip 8 obtained by the chip sensing component 2 .

[0226] A cell counting method comprises the following steps:

[0227] Step 1: Place the cell counting chip 8 at approximately the working distance from the front end of the cell counting module lens 301.

[0228] Step 2: The chip sensing component 2 automatically detects and obtains the position and posture information of the cell counting chip 8.

[0229] Step 3: The auto-focus component control module 702 drives the movement direction and displacement of the auto-focus component 5 according to the position and posture signal of the cell counting chip 8 obtained by the chip sensing component 2 .

[0230] Step 4: Collect cell images and calculate cell concentration and viability.

[0231] Embodiment 4

[0232] A miniature modular cell counter, comprising an illumination component 1, a chip sensing component 2, a microscopic imaging component 3, a central control component 7, an autofocus component 5, a multi-region scanning component 6 and a central control component 7, see Figure 1 ;

[0233] The lighting assembly 1 includes a light source 101, a light homogenizing device 112, a first reflector 102, two focusing units 104, a second reflector 108 and an aperture 109. Figure 5 Among them, the light source 101 is a high-power LED lamp bead with a power of 3W and a voltage of 3.7-5V.

[0234] The light homogenizing device 112 is a complex eye lens with a focal length of 25 mm.

[0235] The two focusing units 104 are lenses with focal lengths of 15 mm and 30 mm respectively.

[0236] The first reflector 102 and the second reflector 108 are right-angle reflective prisms, and the surfaces thereof are coated with AG total reflection films.

[0237] The aperture of the aperture 109 is 10 mm. Figure 2 and Figure 5 .

[0238] The chip sensing component 2 is a diffuse reflection photoelectric switch, which can obtain information about whether there is a cell counting chip 8 in front of the lens 301.

[0239] The microscopic imaging assembly 3 includes a lens 301 , a third reflecting prism 305 , a tube lens 302 and a camera 303 .

[0240] The lens 301 is an infinite plan achromatic objective lens with 10 times magnification, and the tube lens 302 is an achromatic infinite correction lens with a focal length of f=180 mm.

[0241] The third reflecting prism 305 is a right-angle reflecting prism, and the surface of the third reflecting prism is coated with an AG total reflection film.

[0242] Camera 303 is a 6-megapixel CCD camera with a pixel size of 2.0 μm × 2.0 μm. Fig.23 .

[0243] The autofocus component 5 is a linear module, which includes a first power mechanism 502, a first transmission mechanism 506, a first guide mechanism 507, and a first position feedback mechanism 508. The first power mechanism 502 is a 28 stepper motor, and the first transmission mechanism 506 is a screw nut structure with a lead of 2 mm.

[0244] The first guide mechanism 506 is a ball screw structure, and the first position feedback mechanism 508 includes three position pairs of single-point position sensors 5081 and rotation angle sensors 5082. The single-point position sensors 5081 are used as the travel switch and zero point information of the slide, and the rotary encoder is used as the position feedback of the slide. The entire slide is 6.5mm in diameter, and the displacement repeatability is 5μm. Fig. 20 and Fig. 22 .

[0245] The multi-region scanning component 6 is similar to the auto-focus component 5 and is a linear module.

[0246] The central control component 7 includes an image acquisition and data processing module 701, an autofocus component control module 702, a multi-area scanning component control module 707, a chip sensing component control module 703, a data communication module 705 and a human-computer interaction module 706, etc. Fig.18 .

[0247] The image acquisition and data processing module 701 is responsible for functions such as image acquisition, cell identification and viability statistics, and image clarity and sharpness calculation.

[0248] The chip sensing component control module 703 is used to receive the signal detected by the chip sensing component 2, and calculate the existence, position and posture information of the cell counting chip 8 according to the signal, for use by the image acquisition and data processing module 701, the autofocus component control module 702 and the multi-area scanning component control module 707.

[0249] The autofocus component control module 702 drives the movement direction of the autofocus component 5 according to the sharpness and clarity of the collected image. More precisely, it drives the moving focus component to move the lens closer to or away from the cell counting chip 8 according to the change gradient of the sharpness and clarity of the collected image.

[0250] The multi-region scanning component control module 707 is used for the multi-region scanning component 6 to drive the autofocus component 5 to move in a direction parallel to the cell counting chip 8, and to collect cell images during the movement. At the same time, the multi-region scanning component control module 707 also includes a calculation for compensating the focus position during the scanning process according to the position and posture of the cell counting chip 8 obtained by the chip sensing component control module 703.

[0251] A cell counting method comprises the following steps:

[0252] Step 1: Place the cell counting chip 8 at the approximate working distance of the front end of the cell counting module lens 301;

[0253] Step 2: The chip sensing component 2 automatically detects and obtains the position and posture information of the cell counting chip 8;

[0254] Step 3: The image acquisition and data processing module 701 automatically acquires cell images and calculates the clarity and sharpness of the cell images;

[0255] Step 4: The auto-focus component control module 702 automatically calculates the direction and displacement of the next movement of the auto-focus component 5 according to the clarity and sharpness of the cell image;

[0256] Step 5: The image acquisition and data processing module 701 automatically acquires cell images, calculates the clarity and sharpness of the cell images, and determines whether the optimal clarity has been achieved. If not, the process proceeds to step 4.

[0257] Step 6: The image acquisition and data processing module 701 acquires cell images and calculates cell concentration and viability;

[0258] Step 7: The multi-region scanning component 6 drives the auto-focus component 5 to perform multi-region scanning, and at the same time, according to the position and posture information of the cell counting chip 8 obtained by the chip sensing component 2, compensates the focus position of the multi-region scanning component 6 during the process;

[0259] Step 8: Complete multi-area scanning and calculate cell concentration and viability.

[0260] Embodiment 5

[0261] A miniature modular cell counter, characterized by comprising an illumination component 1, a chip sensing component 2, a microscopic imaging component 3 and a central control component 7.

[0262] The lighting assembly 1 includes a light source 101, a light homogenizing device 112, a focusing unit 104, and an aperture 109. Figure 5 .

[0263] The light source 101 is a high-power LED lamp bead with a power of 3W and a voltage of 3.7-5V. The light homogenizing device 112 is a compound eye lens with a focal length of 25mm. The focusing element is a lens with a diameter of 30mm. The aperture 109 has an aperture of 10mm. Fig.19 .

[0264] The chip sensing component 2 is a diffuse reflection photoelectric switch, which can obtain information about whether there is a cell counting chip 8 in front of the lens 301.

[0265] The microscopic imaging assembly 3 includes a lens 301 and a camera 303. The lens is a 10-fold magnification finite element plan achromatic objective lens (tube length 160 mm).

[0266] Camera 303 is a 6-megapixel CCD camera with a pixel size of 2.0 μm × 2.0 μm. Fig.24 .

[0267] The central control component 7 includes an image acquisition and data processing module 701, a chip sensing component control module 703, a data communication module 705 and a human-computer interaction module 706, etc. Fig.18 .

[0268] The image acquisition and data processing module 701 is responsible for image acquisition, cell identification and activity statistics, image clarity and sharpness calculation, etc. The chip sensing component control module 703 is used to receive the signal detected by the chip sensing component 2, and calculate the presence, position and posture information of the cell counting chip 8 according to the signal for use by the image acquisition and data processing module 701.

[0269] A cell counting method comprises the following steps:

[0270] Step 1: Place the cell counting chip 8 at approximately the working distance from the front end of the cell counting module lens 301.

[0271] Step 2: The chip sensing component 2 automatically detects and obtains information that there is a cell counting chip 8 in front of the lens 301.

[0272] Step 3: Collect cell images and calculate cell concentration and viability.

[0273] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of various changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A cell counter, characterized in that: include: The lighting component (1) is used to illuminate the cell counting area, thereby ensuring that the microscopic imaging component (3) obtains an image with uniform illumination; A chip sensing component (2) is used to detect the position information and angle information of the cell counting plate; A microscopic imaging assembly (3), comprising a lens (301), a tube lens (302), a camera (303), an adapter (304) and one or more third reflectors (305), for collecting images of cells in a cell counting chip (8); An automatic focusing assembly (5) is used to drive the lens (301) to move toward or away from the cell counting chip (8); A multi-region scanning component (6) is used to drive the lens (301) and the autofocus component (5) to move in a direction parallel to the cell counting plate; The central control component (7) is used to control image acquisition, automatic focusing, and human-computer interaction equipment.

2. The cell counter according to claim 1, characterized in that: The lighting assembly (1) comprises a light source (101), a first reflector (102), a light source heat dissipation component (103), a plurality of focusing units (104), a plurality of focusing element mounting components (105), a second reflector (108), an aperture (109), a first reflector fixing component (110), a second reflector fixing component (111), a homogenizing device (112), a plurality of fixing components (113) and a guide rail (115), wherein: The light source (101) is arranged on the second reflector fixing part (111), and the light source heat dissipation part (103) is arranged on the second reflector fixing part (111), and the light source heat dissipation part (103) is used to dissipate heat from the light source (101); If the first reflector (102) is evenly arranged on the second reflector fixing part (111), and the homogenizing device (112) is arranged on the second reflector fixing part (111); A plurality of the focusing units (104) are respectively arranged in a plurality of the focusing element mounting components (105), and a plurality of the fixing elements (113) are evenly arranged on the corresponding focusing units (104) and the corresponding focusing element mounting components (105); The outer walls of the plurality of focusing element mounting components (105) are each provided with a sliding groove (114), and the guide rail (115) slides on the inner wall of the sliding groove (114); The second reflector (108) is evenly arranged on the first reflector fixing part (110), and the diaphragm (109) is arranged on the first reflector fixing part (110).

3. The cell counter according to claim 1, characterized in that: The chip sensing component (2) comprises one or more distance sensors (201) for detecting the cell counting chip (8), and the distance sensor (201) is arranged on the microscopic imaging component (3).

4. The cell counter according to claim 1, characterized in that: The cell counting chip (8) is located in front of the lens (301), the tube lens (302) is located behind the lens (301), the tube lens (302) is located in front of the camera (303), the adapter (304) is located in front of the camera (303), the tube lens (302) is located in front of the adapter (304), one or more third reflectors (305) are located between the tube lens (302), the lens (301) and the camera (303), and the lens (301) is arranged on the autofocus assembly (5).

5. The cell counter according to claim 4, characterized in that: The automatic focusing assembly (5) comprises a first mounting seat (501), a first power mechanism (502), a second mounting seat (503); a third mounting seat (504), a fourth mounting seat (505), a first transmission mechanism (506), a first guide mechanism (507) and a first position feedback mechanism (508), wherein: The first power mechanism (502) is arranged on the first mounting seat (501), and the third mounting seat (504) is arranged on the lower surface of the second mounting seat (503); The lens (301) is arranged on the outer wall of the second mounting seat (503), and the fourth mounting seat (505) is adapted to the third mounting seat (504); The first guiding mechanism (507) is arranged on a side of the fourth mounting seat (505) adjacent to the third mounting seat (504); The first transmission mechanism (506) is arranged on the first guide mechanism (507), and the first power mechanism (502) is arranged on the first transmission mechanism (506); The first position feedback mechanism (508) is arranged on the outer wall of the fourth mounting seat (505); The multi-region scanning component (6) is arranged on the lower surface of the fourth mounting seat (505).

6. The cell counter according to claim 5, characterized in that: The multi-region scanning assembly (6) comprises a fifth mounting seat (601), a second power mechanism (602), a sixth mounting seat (604), a seventh mounting seat (605), a second transmission mechanism (606), a second guide mechanism (607) and a second position feedback mechanism (608), wherein: The second power mechanism (602) is arranged on the fifth mounting seat (601), and the second power mechanism (602) is arranged on the second transmission mechanism (606); The sixth mounting seat (604) is matched with the seventh mounting seat (605), and the second guide mechanism (607) is arranged on a side adjacent to the sixth mounting seat (604) and the seventh mounting seat (605); The second position feedback mechanism (608) is arranged on the outer wall of the seventh mounting seat (605), and the second position feedback mechanism (608) has the same structure as the first position feedback mechanism (508); The sixth mounting seat (604) is arranged on the lower surface of the fourth mounting seat (505).

7. The cell counter according to claim 5, characterized in that: The first position feedback mechanism (508) includes a plurality of single-point position sensors (5081) and a rotation angle sensor (5082), wherein: The rotation angle sensor (5082) is arranged on the first power mechanism (502); A plurality of the single-point position sensors (5081) are evenly arranged on the outer wall of the fourth mounting seat (505).

8. The cell counter according to claim 5, characterized in that: The first position feedback mechanism (508) further includes a continuous displacement sensor (5083), wherein: The continuous displacement sensor (5083) is arranged on the outer wall of the third mounting seat (504).

9. The cell counter according to claim 1, characterized in that: The central control component (7) comprises an image acquisition and data processing module (701), an autofocus component control module (702), a chip sensing component control module (703), a data communication module (705), a human-computer interaction module (706) and a multi-region scanning component control module (707), wherein: The image acquisition and data processing module 701 is electrically connected to the chip sensing component (2) and the microscopic imaging component (3) through wires; The automatic focusing component control module (702) is electrically connected to the automatic focusing component (5) via an electric wire; The chip sensing component control module (703) is electrically connected to the chip sensing component (2) via an electric wire; The multi-region scanning component control module (707) is electrically connected to the multi-region scanning component (6) via an electric wire; The data communication module (705) is electrically connected to the image acquisition and data processing module (701), the autofocus component control module (702), the chip sensing component control module (703), the human-computer interaction module (706) and the multi-region scanning component control module (707) through wires.

10. A cell counting method, characterized in that: The following steps are involved: Step 1: Place the cell counting chip (8) at approximately the working distance from the front end of the cell counting module lens (301); Step 2: The chip sensing component (2) automatically detects and obtains the position and posture information of the cell counting chip (8); Step 3: The image acquisition and data processing module (701) automatically acquires cell images and calculates the clarity and sharpness of the cell images; Step 4: The auto-focus component control module (702) automatically calculates the direction and displacement of the next movement of the auto-focus component (5) according to the clarity and sharpness of the cell image; Step 5: The image acquisition and data processing module (701) automatically acquires cell images, calculates the clarity and sharpness of the cell images, and determines whether the optimal clarity has been achieved. If not, the process proceeds to step 4. Step 6: The image acquisition and data processing module (701) acquires cell images and calculates cell concentration and viability; Step 7: The multi-region scanning component (6) drives the auto-focus component (5) to perform multi-region scanning, and at the same time, compensates for the focus position of the multi-region scanning component (6) during the process according to the position and posture information of the cell counting chip (8) obtained by the chip sensing component (2); Step 8: Complete multi-area scanning and calculate cell concentration and viability.

Images