Gene detection kit and method for early accurate diagnosis of atrial fibrillation

Abstract

The invention relates to the technical field of medical apparatus and instruments, and discloses a gene detection kit for early accurate diagnosis of atrial fibrillation, comprising: a temperature control assembly installed inside a kit body and used for controlling the temperature of a gene sample transfer environment; the oscillation assembly is positioned on one side of the inner wall of the box body and is used for performing micro oscillation on the gene sample; the oscillation assembly comprises a motor, the motor is installed on one side of the outer wall of the box body and used for providing driving and reducing the influence of heat dissipation on the internal temperature of the box body, a first rotating shaft is fixed to the output end of the motor, and first gears are arranged on the opposite sides of the inner wall of the box body. Through meshing transmission of the first rotating shaft, the gear and the rotating rod, the lifting plate and the oscillating rod are driven to realize uniform mixing of samples, the design can effectively prevent the precipitation and layering phenomena of the samples in the transfer and treatment process, sufficient mixing of the samples and reagents is ensured, and then the accuracy and reliability of gene detection are improved.

Description

Technical Field

[0001] The present invention relates to the field of medical device technology, and specifically to a gene detection kit and method for early and accurate diagnosis of atrial fibrillation. Background Art

[0002] Atrial fibrillation (AF) is a common arrhythmia, the occurrence of which is affected by many factors, including genetic factors. Studies have found that certain gene variants are significantly associated with the occurrence of AF. For example, variants in genes such as KCNE1, SCN5A, PITX2, GJA5, and KCNQ1 may increase an individual's risk of AF.

[0003] The principle of genetic testing technology is based on detecting specific gene variants associated with atrial fibrillation in an individual's genome. Genetic testing technology can identify these variants and provide valuable information about an individual's risk of atrial fibrillation.

[0004] Although the existing genetic testing kits for early and accurate diagnosis of atrial fibrillation have made some progress in practical applications, there are still some problems that need to be solved:

[0005] Traditional genetic testing kits usually rely on oscillating motors to achieve mixing of samples and reagents. However, this method may generate a lot of heat during operation, resulting in unstable temperature inside the box, thus affecting the integrity of the sample and the reaction effect of the reagents. Temperature fluctuation is a key influencing factor in the genetic testing process. Too high or too low temperature may cause degradation of genetic samples or inactivation of enzymes, ultimately affecting the accuracy of the test results. In addition, the noise and vibration of the oscillating motor may also interfere with the experimental environment.

[0006] In addition, during the sample transfer and processing, traditional methods usually use manual or simple mechanical devices to fix the test tubes and mix the samples, which is inconvenient to operate, inefficient, and has large human errors. Especially when using test tubes of different specifications, the fixation and sealing effects are often not ideal, which may cause leakage or contamination of the sample during the shaking or transfer process, thus affecting the reliability of the test.

[0007] In addition, existing temperature control methods mainly rely on external cooling devices or simple ice packs for temperature control, which are not flexible in operation and difficult to maintain temperature stability for a long time. Due to inaccurate temperature control, samples may be affected by changes in external ambient temperature during transfer and processing, thus affecting the stability of the samples and the reliability of the test results.

[0008] To this end, those skilled in the art provide a genetic testing kit and method for early and accurate diagnosis of atrial fibrillation to solve the problems raised in the above background technology. Summary of the invention

[0009] In view of the deficiencies of the prior art, the present invention provides a gene detection kit and method for early and accurate diagnosis of atrial fibrillation, which solves the problems of poor temperature control efficiency, inactivation during sample transfer and temperature instability in the prior art during use.

[0010] To achieve the above objectives, the present invention is implemented through the following technical solutions: a gene detection kit for early and accurate diagnosis of atrial fibrillation, comprising:

[0011] A temperature control component, which is installed inside the box body and is used to control the temperature of the gene sample transfer environment;

[0012] An oscillating component, which is located on one side of the inner wall of the box body and is used to perform micro-oscillation on the gene sample;

[0013] The oscillation component includes a motor, which is installed on one side of the outer wall of the box body, for providing drive and reducing the influence of heat dissipation on the temperature inside the box body, a first rotating shaft is fixed to the output end of the motor, a first gear is provided on the opposite side of the inner wall of the box body, and a plurality of rotating rods are installed at relative horizontal heights of the first gears, for meshing and transmitting with a gear ring sleeved on the outer wall of the first rotating shaft, and the arrangement of the rotating rod around an eccentric point causes the lifting plate to move up and down according to the stroke groove provided on the inner wall of the box body, positioning plates are provided on both sides of the lifting plate, a positioning plate is fixed on the top of the lifting plate, a plurality of oscillation rods made of rubber material are provided on the top of the positioning plate, and a positioning component is provided inside the box body;

[0014] The sealing component is located at the connection between the oscillating component and the box body, and is used to cooperate with the oscillating component and keep the inside of the genetic sample sealed.

[0015] Preferably, the positioning component includes a reinforcement plate, which is fixed to one side of the inner wall of the box body and has a plurality of conical grooves on the top, and a plurality of L-shaped plates are installed in the inner cavity of each conical groove. The plurality of L-shaped plates are symmetrically distributed around the center of a circle, and a pressure plate is rotatably arranged at the center of the circle. When test tubes of different thicknesses are placed on the top of the pressure plate, the pressure plate is pressed down by their own weight, and the rotation is coordinated to cause the clamping block to contact the tube wall.

[0016] Preferably, the sealing assembly includes a plurality of vertical rods, which are arranged at the four corners of the inner wall of the box body, and a first spring is sleeved on the top of the outer wall thereof; a balance plate is provided on the outer wall of the vertical rod; a lifting plate is fixed on the opposite side of the two balance plates; a plurality of second springs are provided in the inner cavity of the lifting plate for pressing down a T-shaped plate sliding on the inner wall of the lifting plate; a rubber block is installed on one side of the T-shaped plate, and a plurality of elliptical grooves are arranged equidistantly on the bottom of the rubber block; and a decompression component is provided on one side of the lifting plate.

[0017] Preferably, the decompression component comprises a connecting rope, the connecting rope is bolted to the top center point of the lifting plate, and a lifting rod is bolted to one side of the connecting rope.

[0018] Preferably, the temperature control assembly includes a carrying handle, which is located on the top of the box body and fixed by bolts, and is used to lift the entire box body for spatial transfer.

[0019] Preferably, the temperature control assembly includes an energy storage battery, which is arranged on one side of the box body and is used to supply power to the temperature control panel and the motor. Dry ice storage boxes are installed on both sides of the box body through hinges.

[0020] Preferably, the dry ice storage box is filled with solid or block dry ice, and a plurality of ventilation grooves are provided on the inner wall of the dry ice storage box to maintain the internal temperature of the box.

[0021] Preferably, both the front and rear sides of the box body are provided with door grilles through hinges, which are used for taking out the reagent tubes and keeping the interior of the box body sealed in the closed state.

[0022] Preferably, the plurality of oscillation rods, pressure plates and elliptical grooves are the same in number and are horizontally and vertically arranged.

[0023] A method for using a gene detection kit for early and accurate diagnosis of atrial fibrillation, which is used for the gene detection kit for early and accurate diagnosis of atrial fibrillation according to any one of claims 1 to 9, is characterized in that it comprises the following steps:

[0024] S1. Unpacking inspection and preparation: Open the test kit and check whether all components are intact, including the temperature control component, oscillation component, sealing component, and dry ice storage box. Fill the dry ice storage box of the temperature control component with solid or block dry ice, and check the power of the energy storage battery to ensure that the temperature control panel and motor are powered normally;

[0025] S2. Sample collection and loading: Collect the gene sample to be tested, put the sample into the test tube, put the test tube into the positioning component inside the box, the conical groove and L-shaped plate on the reinforcement plate will automatically adjust, and the pressure plate and clamp block will ensure the stability of the test tube;

[0026] S3, Sealing operation: Ensure that the sealing assembly is installed correctly, multiple uprights and the first spring keep the balance plate and the lifting plate stable, the T-plate and the rubber block are pressed down by the second spring, and the elliptical groove ensures the top of the test tube is sealed;

[0027] S4, temperature control start: start the temperature control component, the temperature control panel starts working, the energy storage battery provides power, and the dry ice provides cooling through the ventilation slot to ensure that the temperature inside the box is appropriate;

[0028] S5, start the oscillation component: start the oscillation component, the motor drives the gear ring and the first gear through the first rotating shaft, the rotating rod and the lifting plate move up and down, and the oscillation rod starts to oscillate slightly to ensure that the sample is in a dynamic state and remains active;

[0029] S6. Sample processing: Open the gates on both sides of the box, pull the lever to take out the corresponding test tube according to the gene detection steps for DNA extraction and PCR amplification. During the whole process, keep the temperature and vibration of the remaining samples in the kit to prevent sample degradation and stratification;

[0030] S7. Cleaning and maintenance: After use, clean all reusable components to ensure the normal operation of the temperature control and vibration systems. Regularly maintain the energy storage batteries and dry ice storage boxes to ensure that the equipment is fully functional the next time it is used.

[0031] Working principle: The temperature control component is installed inside the box and is used to control the temperature of the genetic sample transfer environment to ensure that the sample maintains an appropriate temperature when transferred between different devices.

[0032] The oscillation component is located on one side of the inner wall of the box body, and is used to micro-oscillate the genetic sample to prevent the sample from settling and stratifying during the transfer process. The motor is installed on one side of the outer wall of the box body to provide drive, and a first rotating shaft is fixed to the output end of the motor. The motor is installed on one side of the outer wall of the box body to reduce the impact of heat dissipation on the internal temperature of the box body. The first rotating shaft is meshed with the first gear on the opposite side of the inner wall of the box body through the gear ring, driving the rotating rod to rotate around the eccentric point, causing the lifting plate to move up and down according to the stroke groove of the inner wall of the box body. Positioning plates are provided on both sides of the lifting plate, and a plurality of oscillation rods made of rubber are fixed on the top of the lifting plate. The samples in the test tube are gently mixed through the micro-oscillation of the oscillation rods.

[0033] The positioning component includes a reinforcement plate, which is fixed to one side of the inner wall of the box body. A plurality of conical grooves are opened on the top. A plurality of L-shaped plates are installed in each conical groove. The L-shaped plates are symmetrically distributed around the center of the circle. A pressure plate is arranged at the center of the circle. After test tubes of different thicknesses are placed on the top of the pressure plate, the pressure plate is pressed down by its own weight so that the clamping block contacts the tube wall to ensure the stability of the test tube.

[0034] The sealing assembly is located at the connection between the oscillation assembly and the box body, and includes multiple vertical rods. The vertical rods are arranged at the four corners of the inner wall of the box body. A first spring is sleeved on the top of the outer wall. A balance plate is arranged on the outer wall of the vertical rod. A lifting plate is fixed on the opposite side of the two balance plates. A plurality of second springs are arranged in the inner cavity of the lifting plate to press down the T-shaped plate sliding on the inner wall of the lifting plate. A rubber block is installed on one side of the T-shaped plate, and a plurality of elliptical grooves are provided at the bottom of the rubber block to ensure the sealing of the test tube.

[0035] The decompression component includes a connecting rope, which is bolted to the top center point of the lifting plate. A lifting rod is bolted to one side of the connecting rope, and the lifting rod is used to realize the decompression operation, so as to facilitate the removal of the test tube. The carrying handle is located at the top of the box body and is fixed by bolts. It is used to lift the box body as a whole for spatial transfer, so as to facilitate the transfer of samples between different devices.

[0036] The energy storage battery is arranged on one side of the box body to supply power to the temperature control panel and the motor. Dry ice storage boxes are installed on both sides of the box body through hinges. The dry ice storage boxes are filled with solid or block dry ice. The inner wall of the box body is provided with multiple ventilation grooves to maintain the internal temperature of the box body.

[0037] The door grid is hingedly arranged at the front and rear sides of the box body, and is used to take out the reagent tubes and keep the inside of the box body sealed in the closed state, ensuring that the samples are not affected by the external environment during the transfer process.

[0038] The present invention provides a gene detection kit and method for early and accurate diagnosis of atrial fibrillation. It has the following beneficial effects:

[0039] 1. The oscillation component design of the present invention adopts a motor-driven micro-oscillation method. Through the meshing transmission of the first rotating shaft, gears and rotating rod, the lifting plate and the oscillation rod are driven to achieve uniform mixing of the sample. This design can effectively prevent the precipitation and stratification of the sample during the transfer and processing process, ensure the full mixing of the sample and the reagent, and thus improve the accuracy and reliability of genetic testing. Traditional oscillation methods usually rely on oscillation motors, which may affect the internal temperature. The present invention adopts an externally mounted motor to achieve oscillation through mechanical transmission, avoiding the influence of motor heat dissipation on the internal temperature, thereby ensuring the accuracy and stability of temperature control. Compared with the traditional motor oscillation method, this design not only improves the operating efficiency, but also reduces the influence of temperature fluctuations on the sample, ensuring the accuracy and reliability of the test results.

[0040] 2. The combined use of the sealing component and the positioning component of the present invention ensures the stability and sealing of the test tube during the entire operation process. The positioning component can adapt to test tubes of different specifications and provide stable support through the design of the reinforcement plate, L-shaped plate and pressure plate; while the sealing component realizes effective sealing of the test tube through the combination of the vertical rod, balance plate, lifting plate and rubber block to prevent the entry of external contaminants. This design not only ensures the stability of the sample during the shaking and temperature control operations, but also ensures the integrity and cleanliness of the sample, thereby improving the reliability and accuracy of the detection.

[0041] 3. Through the combination of the temperature control component and the dry ice storage box, the test kit of the present invention can provide a stable low-temperature environment during the transfer and processing of genetic samples. This temperature control design not only ensures the constant temperature of the sample during operation and prevents the sample from being degraded due to temperature fluctuations, but also the configuration of the energy storage battery ensures the continuous operation of the temperature control system in the absence of an external power supply, thereby improving the stability of sample preservation and the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 A perspective view of the present invention;

[0043] Figure 2 It is a schematic diagram of the internal structure of the box body of the present invention;

[0044] Figure 3 It is a schematic diagram of the planar structure of the box body of the present invention;

[0045] Figure 4 This is a schematic diagram of the rubber block structure distribution in an upward-looking view of the present invention;

[0046] Figure 5 A schematic diagram of the top view of the reinforcement plate structure distribution of the present invention;

[0047] Figure 6 It is a schematic diagram of the three-dimensional structure of the L-shaped plate of the present invention;

[0048] Figure 7 It is a schematic diagram of the cross-sectional structure of a box body of the present invention;

[0049] Figure 8 It is an exploded schematic diagram of a side lifting plate structure of the present invention;

[0050] Fig. 9 It is an exploded schematic diagram of a side lifting plate structure of the present invention;

[0051] Fig.10 It is a schematic diagram of the cross-sectional structure of the lifting plate of the present invention;

[0052] Fig.11 It is a diagram of the method steps of the present invention.

[0053] Among them, 1. box body; 2. handle; 3. motor; 4. first rotating shaft; 5. gear ring; 6. first gear; 7. rotating rod; 8. lifting plate; 9. positioning plate; 10. oscillating rod; 11. travel groove; 12. reinforcement plate; 13. L-shaped plate; 14. pressure plate; 15. clamping block; 16. conical groove; 17. vertical pole; 18. first spring; 19. balance plate; 20. lifting plate; 21. elliptical groove; 22. rubber block; 23. T-shaped plate; 24. second spring; 25. lifting rod; 26. connecting rope; 27. dry ice storage box; 28. ventilation groove; 29. ​​energy storage battery; 30. temperature control panel. DETAILED DESCRIPTION

[0054] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0055] Embodiment 1:

[0056] The present invention provides a gene detection kit for early and accurate diagnosis of atrial fibrillation, comprising:

[0057] Please refer to the attached Figure 1 -Attached Figure 3 , a temperature control component is installed inside the box body 1 and is used to control the temperature of the gene sample transfer environment; the temperature control component includes a handle 2, which is located on the top of the box body 1 and is fixed by bolts, and is used to lift the box body 1 as a whole for spatial transfer.

[0058] The temperature control component includes an energy storage battery 29, which is arranged on one side of the box body 1 and is used to supply power to the temperature control panel 30 and the motor 3. Dry ice storage boxes 27 are installed on both sides of the box body 1 through hinges. The dry ice storage box 27 is filled with solid or block dry ice. A plurality of ventilation grooves 28 are provided on the inner wall of the box body 1 corresponding to the dry ice storage box 27 to maintain the internal temperature of the box body 1.

[0059] Specifically, the temperature control component is installed inside the box body 1, and is used to control the temperature of the genetic sample transfer environment, ensure the stability of the sample environment temperature during the entire detection process, and avoid the impact of temperature fluctuations on the degradation of genetic samples and the reaction effect of reagents. The temperature control component includes a handle 2, which is located on the top of the box body 1 and fixed by bolts, and is used to lift the box body 1 as a whole for spatial transfer. The design of the handle 2 makes it easier to move the entire test kit between different equipment or laboratories, reduces the vibration and temperature changes that may be caused to the internal samples during the transfer process, and thus improves the stability of sample preservation.

[0060] The temperature control assembly also includes an energy storage battery 29, which is arranged on one side of the box body 1 and is used to supply power to the temperature control board 30 and the motor 3. The existence of the energy storage battery 29 ensures that the temperature control system and the oscillation system can continue to work in the absence of an external power supply, ensuring that the sample environment temperature remains constant throughout the entire detection process. In particular, during transportation, the energy storage battery 29 can maintain the operation of the temperature control assembly, prevent the sample temperature from rising or falling, and thus maintain the integrity of the sample.

[0061] In order to further ensure the temperature stability, dry ice storage boxes 27 are installed on both sides of the box body 1 through hinges. The dry ice storage box 27 is filled with solid or block dry ice, and a plurality of air permeable grooves 28 are provided on the inner wall of the box body 1 corresponding to the dry ice storage box 27. These air permeable grooves 28 are used to keep the temperature inside the box body 1 constant. Dry ice provides a continuous low temperature environment, effectively preventing the temperature of the sample from rising during the processing and transfer process, and is particularly suitable for long-term sample storage and transportation.

[0062] Please refer to the attached Figure 7 -Attached Fig. 9 , an oscillation component, which is located on one side of the inner wall of the box body 1 and is used to perform micro-oscillation on the gene sample;

[0063] The oscillation component includes a motor 3, which is installed on one side of the outer wall of the box body 1 to provide drive and reduce the influence of heat dissipation on the internal temperature of the box body 1. A first rotating shaft 4 is fixed to the output end of the motor 3, and a first gear 6 is provided on the opposite side of the inner wall of the box body 1. A rotating rod 7 is installed at the relative horizontal height of multiple first gears 6, which is used to mesh with a gear ring 5 sleeved on the outer wall of the first rotating shaft 4 for transmission, and the rotating rod 7 is arranged around the eccentric point to cause the lifting plate 8 to move up and down according to the stroke groove 11 provided on the inner wall of the box body 1. Positioning plates 9 are provided on both sides of the lifting plate 8, and a positioning plate 9 is fixed on the top of the lifting plate 8. A plurality of oscillation rods 10 made of rubber material are provided on the top of the positioning plate 9, and a positioning component is provided inside the box body 1;

[0064] Please refer to the attached Figure 3 The number of the multiple vibration rods 10, the pressure plates 14, and the elliptical grooves 21 are the same and they are horizontal and vertical.

[0065] Specifically, the oscillation component is installed on one side of the inner wall of the box body 1, and is used to perform micro-oscillation on the genetic sample. The oscillation component includes a motor 3, which is installed on one side of the outer wall of the box body 1, and keeps the internal environment constant by reducing the influence of heat dissipation on the internal temperature of the box body 1. A first rotating shaft 4 is fixed to the output end of the motor, and is meshed with a first gear 6 on the inner wall of the box body 1 through a gear ring 5. Rotating rods 7 are installed at the relative horizontal heights of multiple first gears 6, and these rotating rods are arranged around an eccentric point to prompt the lifting plate 8 to move up and down according to the travel groove 11 on the inner wall of the box body 1.

[0066] Positioning plates 9 are provided on both sides of the lifting plate 8, and a plurality of rubber oscillating rods 10 are fixed on the top. Through the micro-oscillation of these oscillating rods 10, the uniform mixing of the sample in the test tube is ensured, the precipitation and stratification during the transfer process are avoided, and the accuracy and reliability of the test results are guaranteed.

[0067] Please refer to the attached Figure 2 , Attachment Figure 5 , Attachment Figure 6 The positioning component includes a reinforcing plate 12, which is fixed to one side of the inner wall of the box body 1 and has a plurality of conical grooves 16 on the top. A plurality of L-shaped plates 13 are installed in the inner cavity of each conical groove 16. The plurality of L-shaped plates 13 are symmetrically distributed around the center of the circle. A pressing plate 14 is rotated at the center of the circle. When test tubes of different thicknesses are placed on the top of the pressing plate 14, the pressing plate 14 is pressed down by its own weight, and the clamping block 15 is caused to contact the tube wall in coordination with the rotation.

[0068] Specifically, the positioning component includes a reinforcement plate 12, which is fixed to one side of the inner wall of the box body 1 to provide a stable support structure to ensure that the entire system remains stable during use. A plurality of conical grooves 16 are provided on the top of the reinforcement plate 12. The design of these conical grooves 16 helps to firmly place the test tube in the vertical direction to prevent it from sliding or tilting during operation. The inner cavity of each conical groove 16 is equipped with a plurality of L-shaped plates 13, which are symmetrically distributed around the center of the circle. This symmetrical distribution ensures the stability of the test tube in the conical groove 16 and can adapt to test tubes of different thicknesses. When the test tube is placed on the top of the pressing plate 14, the weight of the test tube itself will cause the pressing plate 14 to move downward. The pressing plate 14 presses the plurality of L-shaped plates 13 inward through its own rotation and downward pressing action, so that the L-shaped plates 13 can firmly contact and fix the outer wall of the test tube. This design not only ensures the stability of the test tube in the shaking and temperature control operation, but also ensures the compatibility of test tubes of different specifications. The rotation of the pressing plate 14 drives the clamping block 15 to contact the wall of the test tube, providing additional fixing force to prevent the test tube from shifting or shaking during use, thereby improving the accuracy and reliability of sample processing. The overall structural design effectively reduces the possibility of the test tube moving or tilting, ensuring the stability of the test tube during operation, thereby improving the accuracy and consistency of genetic sample detection.

[0069] Please refer to the attached Fig.10 , Attachment Figure 3 , Attachment Figure 4 , a sealing component, which is located at the connection between the oscillating component and the box body 1, is used to cooperate with the oscillating component and keep the internal sealing of the genetic sample.

[0070] The sealing assembly includes a plurality of vertical rods 17, which are arranged at the four corners of the inner wall of the box body 1, and a first spring 18 is sleeved on the top of the outer wall thereof. A balance plate 19 is arranged on the outer wall of the vertical rod 17, and a lifting plate 20 is fixed on the opposite side of the two balance plates 19. The inner cavity of the lifting plate 20 is provided with a plurality of second springs 24 for pressing down a T-shaped plate 23 sliding on the inner wall of the lifting plate 20, and a rubber block 22 is installed on one side of the T-shaped plate 23, and a plurality of elliptical grooves 21 are arranged equidistantly on the bottom of the rubber block 22, and a decompression component is arranged on one side of the lifting plate 20.

[0071] Specifically, the sealing component is located at the connection between the oscillating component and the box body 1, and is designed to cooperate with the oscillating component during operation and maintain the internal sealing of the genetic sample to prevent the external environment from contaminating and interfering with the sample. The core structure of the sealing component includes a plurality of vertical rods 17, which are evenly arranged at the four corners of the inner wall of the box body 1 to ensure the stability of the entire sealing system. A first spring 18 is sleeved on the top of the outer wall of each vertical rod 17, and the first spring 18 provides the necessary elastic force to support and maintain the stability of the vertical rod 17.

[0072] The outer wall of the vertical rod 17 is provided with a balance plate 19, and two balance plates 19 are fixed on opposite sides of the vertical rod 17. The design of the balance plate 19 ensures the balance and stability of the entire system during the oscillation process, thereby preventing the sample from being shaken unnecessarily during the sealing process. The balance plate 19 is connected to the lifting plate 20, and the inner cavity of the lifting plate 20 is provided with a plurality of second springs 24, which are used to provide additional elastic force to ensure that the T-shaped plate 23 can maintain the necessary pressure and sealing when sliding on the inner wall of the lifting plate 20.

[0073] A rubber block 22 is installed on one side of the T-shaped plate 23. A plurality of elliptical grooves 21 are arranged at equal intervals on the bottom of the rubber block 22. The design of these elliptical grooves 21 enables the rubber block 22 to flexibly adjust and adapt to test tubes of different sizes to achieve precise sealing. The rubber material ensures the flexibility and tightness of the seal, preventing any external air or contaminants from entering the sample area.

[0074] A decompression component is provided on one side of the lifting plate 20, and the decompression component includes a connecting rope 26, which is bolted to the top center point of the lifting plate 20, and the decompression operation is realized through a lifting rod 25. When it is necessary to take out the test tube or process the sample, the operator can easily release the sealing state by pulling the lifting rod 25 on the connecting rope 26, so as to facilitate taking out or processing the sample.

[0075] This sealing component design has significant advantages in maintaining the stability and cleanliness of the internal environment of the sample. Through the design of multiple springs and flexible rubber blocks 22, the safety and sealing of the sample during operation are ensured, effectively improving the accuracy and reliability of genetic testing.

[0076] Please see attached Figure 1 -Attached Figure 3 The decompression component includes a connecting rope 26, which is bolted to the top center point of the lifting plate 20, and a lifting rod 25 is bolted to one side of the connecting rope 26.

[0077] In actual operation, the box body 1 is first transferred as a whole through the handle 2. The temperature control component is started, the energy storage battery 29 provides power support, and the dry ice storage box 27 ensures a low temperature environment. The sample is loaded into the positioning component and fixed by the reinforcement plate 12, the L-shaped plate 13 and the pressure plate 14. The oscillation component is started, the motor 3 drives the first shaft 4, the gear 6, the rotating rod 7 and the lifting plate 8, and the oscillation rod 10 performs micro-oscillation. The sealing component ensures the sealing of the sample, and the decompression component connecting rope 26 and the lifting rod 25 facilitate the removal of the sample.

[0078] Specifically, the decompression component includes a connecting rope 26, which is bolted to the top center point of the lifting plate 20 to ensure that the lifting plate 20 can be stably lifted or lowered when subjected to external force. A lifting rod 25 is bolted to one side of the connecting rope 26, and the design of the lifting rod 25 allows the operator to easily pull the connecting rope 26 to control the lifting plate 20. In actual operation, this design provides a simple decompression and sampling method, avoids complex mechanical operations, and improves work efficiency.

[0079] In actual operation, the box body 1 is first transferred as a whole through the handle 2 to ensure the stability of the reagent box and the safety of the sample when moving between different devices. The temperature control component is started, and the energy storage battery 29 provides power support to ensure that the temperature control board 30 and the motor 3 can operate normally without an external power supply. The dry ice storage box 27 ensures a low temperature environment, and through the continuous cooling effect of dry ice, the appropriate temperature inside the box body 1 is maintained to prevent sample degradation.

[0080] Place the sample in the positioning component and fix it with the reinforcement plate 12, L-shaped plate 13 and pressure plate 14. The reinforcement plate 12 provides a stable support structure, and the L-shaped plate 13 is symmetrically distributed around the center of the circle, which can adapt to test tubes of different thicknesses. When the test tube is placed on the top of the pressure plate 14, it uses its own weight to press down the pressure plate 14, causing the clamp 15 to contact the tube wall, thereby ensuring the stability of the test tube during the oscillation and transfer process. Start the oscillation component, the motor 3 drives the first shaft 4, and through the meshing transmission of the gear 6 and the rotating rod 7, drives the lifting plate 8 to move up and down according to the travel groove 11, and the oscillation rod 10 performs micro-oscillation to ensure the uniform mixing of the sample in the test tube and prevent precipitation and stratification.

[0081] The sealing assembly ensures the sealing of the sample, and the combination of the vertical rod 17, the first spring 18, the balance plate 19 and the lifting plate 20 prevents external contaminants from entering the sample area through the sealing effect of the rubber block 22 and the elliptical groove 21. The decompression component connecting rope 26 and lifting rod 25 facilitates the removal of the sample. The operator lifts the lifting rod 25 to release the pressure of the lifting plate 20, so that the T-plate 23 and the rubber block 22 are loosened, so that the test tube can be easily removed for subsequent processing.

[0082] This series of operating steps and component designs ensure temperature stability, uniform mixing of samples and effective sealing during the transfer and processing of genetic samples, thereby improving the accuracy and reliability of genetic testing and providing an efficient and convenient solution for early and precise diagnosis of atrial fibrillation.

[0083] Embodiment 2:

[0084] Please see attached Fig.11 The present invention provides a method for using a gene detection kit for early and accurate diagnosis of atrial fibrillation, comprising the following steps:

[0085] S1. Unpacking inspection and preparation: Open the test kit and check whether all components are intact, including the temperature control component, the oscillating component, the sealing component, and the dry ice storage box 27. Fill the dry ice storage box 27 of the temperature control component with solid or block dry ice, and check the power of the energy storage battery 29 to ensure that the temperature control panel 30 and the motor 3 are powered normally;

[0086] S2. Sample collection and loading: Collect the gene sample to be tested, put the sample into a test tube, and put the test tube into the positioning component inside the box body 1. The conical groove 16 and the L-shaped plate 13 on the reinforcement plate 12 will be automatically adjusted, and the pressing plate 14 and the clamping block 15 will ensure that the test tube is stable;

[0087] S3, sealing operation: ensure that the sealing assembly is correctly installed, multiple uprights 17 and the first spring 18 keep the balance plate 19 and the lifting plate 20 stable, the T-plate 23 and the rubber block 22 are pressed down by the second spring 24, and the elliptical groove 21 ensures the top of the test tube is sealed;

[0088] S4, temperature control start: start the temperature control component, the temperature control panel 30 starts to work, the energy storage battery 29 provides power, and the dry ice provides cooling through the ventilation slot 28 to ensure that the temperature inside the box body 1 is appropriate;

[0089] S5, start the oscillation component: start the oscillation component, the motor 3 drives the gear ring 5 and the first gear 6 through the first rotating shaft 4, the rotating rod 7 and the lifting plate 8 move up and down, and the oscillation rod 10 starts to oscillate slightly to ensure that the sample is in a dynamic state and remains active;

[0090] S6, sample processing: open the gates on both sides of the box body 1, pull the lifting rod 25 according to the gene detection steps to take out the corresponding test tube for DNA extraction and PCR amplification operations, and keep the temperature and vibration of the remaining samples in the test kit during the whole process to prevent sample degradation and stratification;

[0091] S7. Cleaning and maintenance: After use, clean all reusable components to ensure the normal operation of the temperature control and oscillation systems, and regularly maintain the energy storage battery 29 and the dry ice storage box 27 to ensure that the equipment functions properly the next time it is used.

[0092] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A gene detection kit for early and accurate diagnosis of atrial fibrillation, characterized in that: include: A temperature control component, which is installed inside the box body (1) and is used to control the temperature of the gene sample transfer environment; An oscillating component, located on one side of the inner wall of the box body (1), and used to perform micro-oscillation on the gene sample; The oscillation component comprises a motor (3), which is mounted on one side of the outer wall of the box body (1) for providing drive and reducing the influence of heat dissipation on the internal temperature of the box body (1). A first rotating shaft (4) is fixed to the output end of the motor (3). A first gear (6) is provided on the opposite side of the inner wall of the box body (1). A plurality of rotating rods (7) are installed at relative horizontal heights of the first gears (6) for meshing with a gear ring (5) sleeved on the outer wall of the first rotating shaft (4) for transmission. The rotating rod (7) is arranged around an eccentric point to cause the lifting plate (8) to move up and down according to a stroke groove (11) provided on the inner wall of the box body (1). Positioning plates (9) are provided on both sides of the lifting plate (8). A positioning plate (9) is fixed on the top of the lifting plate (8). A plurality of oscillation rods (10) made of rubber material are provided on the top of the positioning plate (9). A positioning component is provided inside the box body (1). The sealing component is located at the connection between the oscillating component and the box body (1) and is used to cooperate with the oscillating component and maintain the internal sealing of the genetic sample.

2. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 1, characterized in that: The positioning component comprises a reinforcing plate (12), the reinforcing plate (12) being fixed to one side of the inner wall of the box body (1) and having a plurality of conical grooves (16) on the top, a plurality of L-shaped plates (13) being installed in the inner cavity of each conical groove (16), the plurality of L-shaped plates (13) being symmetrically distributed around the center of a circle, a pressing plate (14) being rotatably arranged at the center of the circle, when test tubes of different thicknesses are placed on the top of the pressing plate (14), the pressing plate (14) is pressed down by its own weight, and the clamping block (15) is caused to contact the tube wall by the rotation.

3. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 1, characterized in that: The sealing assembly comprises a plurality of vertical rods (17), the plurality of vertical rods (17) being arranged at four corners of the inner wall of the box body (1), the top of the outer wall of which is sleeved with a first spring (18), the outer wall of the vertical rods (17) being provided with a balance plate (19), a lifting plate (20) being fixed on opposite sides of the two balance plates (19), the inner cavity of the lifting plate (20) being provided with a plurality of second springs (24) for pressing down a T-shaped plate (23) sliding on the inner wall of the lifting plate (20), a rubber block (22) being installed on one side of the T-shaped plate (23), a plurality of elliptical grooves (21) being arranged at equal intervals on the bottom of the rubber block (22), and a decompression component being provided on one side of the lifting plate (20).

4. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 3, characterized in that: The decompression component comprises a connecting rope (26), wherein the connecting rope (26) is bolted to the top center point of the lifting plate (20), and a lifting rod (25) is bolted to one side of the connecting rope (26).

5. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 1, characterized in that: The temperature control component comprises a carrying handle (2), which is located on the top of the box body (1) and is fixed by bolts and is used to lift the box body (1) as a whole for spatial transfer.

6. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 1, characterized in that: The temperature control assembly comprises an energy storage battery (29), which is arranged on one side of the box body (1) and is used to supply power to the temperature control panel (30) and the motor (3). Dry ice storage boxes (27) are mounted on both sides of the box body (1) via hinges.

7. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 6, characterized in that: The dry ice storage box (27) is filled with solid or block dry ice, and a plurality of ventilation grooves (28) are provided on the inner wall of the box body (1) corresponding to the dry ice storage box (27) for maintaining the internal temperature of the box body (1).

8. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 5, characterized in that: Door grilles are provided on both the front and rear sides of the box body (1) via hinges, which are used to take out reagent test tubes and keep the interior of the box body (1) sealed in a closed state.

9. The gene detection kit for early and accurate diagnosis of atrial fibrillation according to claim 1, characterized in that: The plurality of oscillating rods (10), pressing plates (14), and elliptical grooves (21) are of the same number and are arranged horizontally and vertically.

10. A method for using a gene detection kit for early and accurate diagnosis of atrial fibrillation, the gene detection kit for early and accurate diagnosis of atrial fibrillation according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Unpacking inspection and preparation: Open the test kit and check whether all components are intact, including the temperature control component, the oscillation component, the sealing component, and the dry ice storage box. Fill the dry ice storage box (27) of the temperature control component with solid or block dry ice, and check the power level of the energy storage battery (29) to ensure that the temperature control panel (30) and the motor (3) are powered normally; S2. Sample collection and loading: Collect the gene sample to be tested, put the sample into a test tube, and put the test tube into the positioning component inside the box body (1). The conical groove (16) and the L-shaped plate (13) on the reinforcement plate (12) will be automatically adjusted, and the pressing plate (14) and the clamping block (15) will ensure that the test tube is stable; S3, sealing operation: ensure that the sealing assembly is correctly installed, multiple vertical rods (17) and the first spring (18) keep the balance plate (19) and the lifting plate (20) stable, the T-plate (23) and the rubber block (22) are pressed down by the second spring (24), and the elliptical groove (21) ensures that the top of the test tube is sealed; S4, temperature control start: start the temperature control component, the temperature control panel (30) starts to work, the energy storage battery (29) provides power, and the dry ice provides cooling through the ventilation slot (28) to ensure that the temperature inside the box body (1) is appropriate; S5, start the oscillation component: start the oscillation component, the motor (3) drives the gear ring (5) and the first gear (6) through the first rotating shaft (4), the rotating rod (7) and the lifting plate (8) move up and down, and the oscillation rod (10) starts to oscillate slightly to ensure that the sample is in a dynamic state and remains active; S6, sample processing: open the gates on the front and rear sides of the box body (1), pull the lifting rod (25) according to the gene detection steps to take out the corresponding test tube for DNA extraction and PCR amplification operations, and keep the temperature and vibration of the remaining samples in the kit during the whole process to prevent sample degradation and stratification; S7. Cleaning and maintenance: After use, clean all reusable components to ensure the normal operation of the temperature control and oscillation systems, and regularly maintain the energy storage battery (29) and dry ice storage box (27) to ensure that the equipment functions properly the next time it is used.

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