Method for preserving vegetative propagation materials and germplasm of zamioculcas zamiifolia through dry tubers
By air-drying and dehydrating the money tree tuber and cold-drying and storage, the problems of fresh tuber being easily damaged and rotten during the handling and storage process are solved, and long-term and high-quality storage of the tuber is achieved, and transportation and logistics costs are reduced. , improves the circulation and application efficiency of propagation materials.
Patent Information
- Application Number
- CN202411978629.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-02-18
AI Technical Summary
In the field of money tree breeding and germplasm preservation, it is difficult for the existing technology to effectively use money tree tuber, a material that is easy to operate and grow quickly, and fresh tuber is easily damaged and rot during transportation and storage, resulting in a large amount of breeding materials. loss.
By air-drying and dehydrating the money tree tuber, the moisture content is reduced to less than 50%, and long-term storage is carried out under cold drying, avoiding light and good ventilation. After the stored dry tuber is rehydrated in a specific matrix, the tuber buds are germinated and the seedlings are formed.
After air-drying and dehydration treatment, the tubers are hard in texture and the internal buds enter a dormant state, which reduces the sensitivity to the storage environment, avoids the risks of germination and rot, and achieves long-term and high-quality storage and storage. At the same time, the weight of tubers is reduced, transportation and logistics costs are reduced, and the circulation and application efficiency of breeding materials is improved.
Smart Images
Figure CN119452809A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of plant vegetative propagation and germplasm preservation, and in particular relates to a method for preserving vegetative propagation materials and germplasm of money tree through dry tubers. Background Art
[0002] Money tree ( Zamioculcas zamiifolia Engl.) is a perennial evergreen herbaceous plant of the genus Araceae, and is an excellent ornamental and medicinal plant. In the field of money tree propagation and germplasm preservation, there was a lack of understanding of the dehydration resistance and vitality retention characteristics of money tree tubers, a key propagation material, and no corresponding propagation material preservation method has been developed. In production, it is directly propagated and retained by cuttings of small leaves and other vegetative organs, and the tubers, which are easy to operate, grow and grow quickly, are not well utilized for propagation. As an introduced plant, it is difficult for money tree to produce seeds under potted conditions, and the seed vitality is also poor, and the germination rate is low. The germplasm preservation of the species mainly relies on potted living plants, which are difficult to manage, costly, and easy to lose. On the other hand, the storage and preservation methods of fresh materials have obvious disadvantages. Fresh tubers have a water content of up to 95% and a soft texture. They are easily damaged during transportation and storage, which leads to rot problems and a large loss of propagation materials. At the same time, fresh tubers are easy to germinate, making it difficult to store tubers stably for a long time, and cannot meet the needs of long-term and long-distance transportation and preservation.
[0003] Furthermore, considering the cost and transportation convenience, unprocessed fresh tubers are heavy, which not only takes up a lot of transportation space and increases logistics costs, but also is inconvenient to carry. This greatly limits the large-scale circulation and reproduction application of Zamioculcas tubers as a breeding material, and also provides new ways and methods for the in vitro preservation of Zamioculcas germplasm. Summary of the invention
[0004] In order to overcome the deficiencies of the prior art, the present invention discloses a method for preserving vegetative propagation materials and germplasm of Zamioculcas zamiifolia by dry tubers, so as to solve many difficulties faced in Zamioculcas zamiifolia propagation and germplasm preservation technology.
[0005] One embodiment of the present invention provides a method for preserving vegetative propagation materials and germplasm of Zamioculcas chinensis by dry tubers, comprising the following steps: Produced from underground parts of Zamioculcas chinensis plants, or cultivated by cutting and propagating small leaflets, leaf axes, tubers and root cuttings, selecting complete tubers that have not sprouted after formation, washing the tubers with clean water and drying them, and air-drying them according to the diameter of the tubers; Dry the tubers of different sizes after classification in a blast drying oven at a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days, or dry them naturally at a temperature not higher than 35°C to remove moisture from the tubers until the moisture content is less than 50%; After drying or air-drying the tubers to a moisture content of 50%, place the tubers in a cool, dry, dark and well-ventilated condition for long-term storage and preservation; The stored dry tubers are buried in sand or peat substrate with a field water holding capacity of more than 80% for rehydration. The tubers begin to germinate about a week after absorbing water and sprout into seedlings, which are used as money tree seedlings.
[0006] In one embodiment, it also includes: Tubers produced from underground parts of money tree plants, small leaves on compound leaves, etc. Sorting tubers by diameter ensures consistency and time accuracy in subsequent dehydration processing; Provide a blast drying box with a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days to reach the required moisture content; Dehydration of dry tubers requires a moisture content of less than 50% but not less than 30%; Store in a cool, dry, light-proof and well-ventilated environment to maintain the activity of the tubers; The stored tubers are rehydrated in a clean river sand or peat substrate at a specific field holding capacity to promote the germination of tuber buds in about a week.
[0007] In one embodiment, it also includes: Obtain tubers by regenerating underground parts of money tree plants or cutting regeneration methods such as small leaves, and lock in target tubers with normal morphology that have not sprouted; After the tubers have reached the moisture content standard through appropriate air-drying methods, they are stored in a cold, dry environment with good ventilation and light-proof conditions; The stored tubers are placed in a sand or peat substrate with a field water holding capacity greater than 80% for rehydration to achieve the formation and growth of buds within the tubers and regenerate into seedlings.
[0008] The method provided by the above technical solution for preserving the vegetative propagation materials and germplasm of Zamioculcas zamiifolia by dry tubers has the following beneficial effects: 1. The tuber air-drying and dehydration treatment method provided by the present invention has simple flow and operation. Only a simple drying machine or air-drying place is needed, and a common blast oven or an ordinary well-ventilated indoor space is used. Ordinary practitioners can easily start the operation without investing a large amount of money to purchase complex and expensive professional equipment, and without spending a lot of time and energy to learn complex operating skills, which greatly reduces the technical threshold and capital investment cost, allowing more people to participate in the early processing of money tree propagation materials.
[0009] 2. The texture of the tubers becomes hard after air drying, and the internal buds or tissues enter a dormant state like the embryo of a seed. This dormant characteristic allows the tubers to maintain a relatively stable state during storage, greatly reducing their sensitivity to storage environmental conditions, avoiding the risk of easy germination or disease attack due to changes in external factors such as temperature, humidity, and microorganisms, thereby creating favorable conditions for long-term, high-quality storage and preservation, ensuring the long-term effectiveness of propagation materials, and providing a solid guarantee for the sustainable use of germplasm resources. The tubers are air-dried to a water content of 50%. Under normal circumstances, the water content of the tubers of the money tree is as high as 95%. Direct storage and transportation are prone to damage and rot under normal conditions, but keeping the tubers at a water content of 50% can solve the above problems well.
[0010] 3. The weight of the processed tubers is greatly reduced, about half, and this change brings multiple benefits: First, in the transportation link, the reduced weight directly means a reduction in the occupied transportation space. Originally, one trip could only transport a limited number of tubers, but now it can transport double the number of air-dried tubers, and the transportation efficiency is doubled; secondly, with the reduction in the demand for transportation space, logistics costs are also greatly reduced, including transportation costs, packaging material costs, etc. can be effectively controlled; thirdly, during the handling process, due to the reduction in the weight of the tubers, the difficulty and labor intensity of manual handling are significantly reduced, and the risk of tuber damage due to improper handling is also reduced, which optimizes the cost-effectiveness of the money tree propagation materials from production to application.
[0011] 4. Air-dried tubers effectively avoid the damage and rot problems that are easy to occur in the transportation and storage of fresh tubers, avoid the deterioration or even scrapping of the quality of the propagation materials due to damage, and the breeding and spread of pathogens caused by rot. It not only reduces the direct loss of propagation materials, but also reduces the additional costs required for subsequent disinfection and prevention and control of pathogenic contamination. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.
[0013] Figure 1 It is a schematic diagram of the tuber of the present invention having a water content of less than 50%; Figure 2 It is a schematic diagram of fresh tubers with a water content of more than 95% in the prior art. DETAILED DESCRIPTION
[0014] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.
[0015] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0016] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.
[0017] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.
[0018] Combination Figure 1 As shown, one embodiment of the present invention provides a method for preserving vegetative propagation materials and germplasm of Zamioculcas chinensis by dry tubers, comprising the following steps: Produced from underground parts of Zamioculcas chinensis plants, or cultivated by cutting and propagating small leaflets, leaf axes, tubers and root cuttings, selecting complete tubers that have not sprouted after formation, washing the tubers with clean water and drying them, and air-drying them according to the diameter of the tubers; Dry the tubers of different sizes after classification in a blast drying oven at a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days, or dry them naturally at a temperature not higher than 35°C to remove moisture from the tubers until the moisture content is less than 50%; After drying or air-drying the tubers to a moisture content of 50%, place the tubers in a cool, dry, dark and well-ventilated condition for long-term storage and preservation; The stored dry tubers are buried in sand or peat substrate with a field water holding capacity of more than 80% for rehydration. The tubers begin to germinate about a week after absorbing water and sprout into seedlings, which are used as money tree seedlings.
[0019] Also includes: Tubers produced from underground production of money tree plants, compound leaflets and other cuttings; Sorting tubers by diameter ensures consistency and time accuracy in subsequent dehydration processing; Provide a blast drying box with a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days to reach the required moisture content; Dehydration of dry tubers requires a moisture content of less than 50% but not less than 30%; Store in a cool, dry, light-proof and well-ventilated environment to maintain the activity of the tubers; The stored tubers are rehydrated in a clean river sand or peat substrate at a specific field holding capacity to promote the germination of tuber buds in about a week.
[0020] Also includes: Obtain tubers by regenerating the ground part of the money tree plant or cutting regeneration methods such as compound leaflets, and lock in target tubers with normal morphology that have not sprouted; After the tubers have reached the moisture content standard through appropriate air-drying methods, they are stored in a cold, dry environment with good ventilation and light-proof conditions; The stored tubers are placed in a sand or peat substrate with a field water holding capacity greater than 80% for rehydration to achieve the formation and growth of buds within the tubers and regenerate into seedlings.
[0021] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the paper document and the contents of the drawings of the present invention under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.
Claims
1. A method for preserving vegetative propagation materials and germplasm of Zamioculcas chinensis by dry tubers, characterized in that: The following steps are involved: Produced from underground parts of Zamioculcas chinensis plants, or cultivated by cutting and propagating small leaflets, leaf axes, tubers and root cuttings, selecting complete tubers that have not sprouted after formation, washing the tubers with clean water and drying them, and air-drying them according to the diameter of the tubers; Dry the tubers of different sizes after classification in a blast drying oven at a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days, or dry them naturally at a temperature not higher than 35°C to remove moisture from the tubers until the moisture content is less than 50%; After drying or air-drying the tubers to a moisture content of 50%, place the tubers in a cool, dry, dark and well-ventilated condition for long-term storage and preservation; The stored dry tubers are buried in sand or peat substrate with a field water holding capacity of more than 80% for rehydration. The tubers begin to germinate about a week after absorbing water and sprout into seedlings, which can be used as seedlings for money tree production or germplasm cultivation.
2. A method for preserving Zamioculcas zamiifolia vegetative propagation materials and germplasm by dry tubers as claimed in claim 1, characterized in that: Also includes: Tubers produced from underground production of money tree plants, compound leaflets and other cuttings; Sorting tubers by diameter ensures consistency and time accuracy in subsequent dehydration processing; Provide a blast drying box with a temperature of 35°C and a wind speed of 1.0m / s for 5-10 days to reach the required moisture content; Dehydration of dry tubers requires a moisture content of less than 50% but not less than 30%; Store in a cool, dry, light-proof and well-ventilated environment to maintain the activity of the tubers; When regenerating, the stored dry tubers are rehydrated in clean river sand or peat substrate with a specific field water holding capacity to promote the germination of tuber buds in about a week.
3. A method for preserving vegetative propagation materials and germplasm of Zamioculcas zamiifolia by dry tubers as claimed in claim 1, characterized in that: Also includes: Obtain tubers by regenerating the underground part of the money tree plant or by cutting regeneration using small leaves on compound leaves, and lock in the target tubers with normal morphology that have not sprouted; After the tubers have reached the moisture content standard through appropriate air-drying methods, they are stored in a cold, dry environment with good ventilation and light-proof conditions; The stored tubers are placed in a sand or peat substrate with a field water holding capacity greater than 80% for rehydration to achieve the formation and growth of buds within the tubers and regenerate into seedlings.