Tumor tissue recovery device in intracranial glioma operation and operation method thereof

Abstract

The invention relates to the technical field of medical equipment, in particular to a tumor tissue recovery device in an intracranial glioma operation, which comprises a base and a suction tube, the base is rotatably sleeved with a centrifugal mechanism, the upper part of the centrifugal mechanism is rotatably connected with a negative pressure suction mechanism, and the negative pressure suction mechanism is connected with the suction tube. A negative pressure environment is formed in the suction pipe through the negative pressure suction mechanism, then the technical effect that the suction pipe sucks tumor tissue is achieved, the recycled tumor tissue is centrifugally filtered through the centrifugal mechanism, and the purity and quality of the collected tumor tissue are higher. And through the arrangement of the constant pressure cabin, the technical problem of wall hanging residues in the mixing process of the tumor tissue and the red blood cell lysis buffer in the prior art is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a device for recovering tumor tissue during intracranial glioma surgery and an operating method thereof. Background Art

[0002] In recent years, with the rapid development of disciplines such as molecular biology and genetic biology, tumor immunotherapy and gene therapy have become new tumor treatment models. Whether it is biological immunotherapy or gene therapy, it is necessary to obtain tumor tissue from the corresponding part for in vitro culture or genetic testing. Obtaining pathological tissue is the core work of making pathological diagnosis in the medical process. For most diseases, it is not too difficult as long as invasive or non-invasive professional means are adopted; for diseases with small lesions, difficult sampling or the sampling process that is easy to cause secondary damage to patients, such as high-grade gliomas, myeloblastomas and other highly malignant tumors, due to the rich blood supply of the tumor, retaining a large amount of tissue at one time is easy to increase bleeding, and excessive traction of brain tissue is easy to cause secondary damage.

[0003] Glioma is one of the most common malignant tumors in the central nervous system. It is highly malignant, grows rapidly, is highly invasive, is prone to recurrence after surgery, and has a high mortality rate. The treatment of this type of tumor has always been a difficult problem in the medical community. The treatment effect of traditional surgery plus radiotherapy and chemotherapy is often unsatisfactory, and the prognosis is poor.

[0004] During glioma surgery, accurate removal of tumor tissue is crucial to the patient's prognosis. However, due to the infiltrative characteristics of glioma, it is very difficult to achieve complete pathological resection. Therefore, how to efficiently and safely recover tumor tissue during surgery for subsequent pathological diagnosis and molecular genetic testing has become an important research direction.

[0005] After searching, the Chinese patent application with application number 202111317854.9 discloses a device for recovering tumor tissue during intracranial glioma surgery, including: a liquid storage bottle, the top of which is closed and connected to an aspirator connecting tube and a washing liquid delivery tube, the washing liquid delivery tube is connected to the washing liquid storage bottle, and is used to deliver the washing liquid to the liquid storage bottle; a negative pressure aspirator, the discharge end of the negative pressure aspirator is connected to the aspirator connecting tube; a centrifugal collection assembly, including a centrifugal device, a centrifugal sleeve installed inside the centrifugal device, and a sampling tube connected to the bottom of the liquid storage bottle, the centrifugal sleeve is connected to the discharge end of the sampling tube. The recovery device of the present application has a simple structure and can collect a large amount of tumor tissue without increasing the patient's physical burden. The recovered tumor tissue can be used for the patient's later diagnosis and treatment process, which can avoid the missed detection of genetic testing due to insufficient tumor tissue sampling or the inability to effectively activate in vitro immune cells due to insufficient patient tumor tissue during later biological treatment.

[0006] When the above recovery device attracts tumor tissue into the liquid storage bottle, due to the lack of a constant pressure device, tumor tissue may remain on the wall and cannot enter the centrifugal cannula completely, resulting in technical problems such as waste and insufficient tumor tissue collection. Summary of the invention

[0007] When the recovery device in the prior art sucks the tumor tissue into the liquid storage bottle, there is no constant pressure device, which may cause the tumor tissue to remain on the wall and fail to enter the centrifugal sleeve, resulting in waste and insufficient technical problems in the process of collecting tumor tissue.

[0008] Technical idea: Based on the problems of the prior art, this application provides a device for recovering tumor tissue during intracranial glioma surgery. The air pump is used to form a negative pressure state in the liquid collecting tank to achieve suction and recovery of the tumor tissue. The constant pressure cabin is set to allow the tumor tissue in the liquid collecting tank to completely enter the centrifugal chamber for centrifugal treatment, ensuring that there is no residue or waste of tumor tissue during the recovery process.

[0009] To achieve the above object, the technical solution adopted by the present invention is: The present application provides a device for recovering tumor tissue during intracranial glioma surgery, comprising a base and a suction tube, wherein a centrifugal mechanism is rotatably sleeved on the base, a negative pressure suction mechanism is rotatably connected above the centrifugal mechanism, and the negative pressure suction mechanism is connected to the suction tube.

[0010] Furthermore, the negative pressure suction mechanism includes a liquid collection tank, an air pump and a constant pressure chamber; The liquid collecting tank is connected to the suction pipe, and a solenoid valve core is provided at the connection; The air pump is arranged above the liquid collecting tank and is connected to the suction pipe, and a waterproof and breathable membrane is arranged at the connection; The constant pressure cabin is arranged above the liquid collecting tank, and the side end of the constant pressure cabin is connected with the air pump, and the bottom end is connected with the liquid collecting tank.

[0011] Furthermore, a cleaning liquid tank is provided on the air pump, and the cleaning liquid tank is connected to the air pump.

[0012] In detail, a through hole is opened at the bottom of the liquid collecting tank and is connected to a delivery pipe. A baffle matching the through hole is slidably arranged on the side wall of the delivery pipe, and a pull rod is connected to the end of the baffle.

[0013] It should be noted that the fluid collection tank contains red blood cell lysis solution, which is used to lyse the blood adhering to the surface of the tumor tissue.

[0014] Specifically, a pressure relief pipe is arranged above the constant pressure cabin, a spring is arranged inside the pressure relief pipe, a pressure relief ball is connected to the spring, and the pressure relief ball cooperates with an opening opened on the wall of the pressure relief pipe.

[0015] In the above technical solution, the centrifugal mechanism includes a centrifugal chamber rotatably connected to the conveying tube and a motor fixedly connected to the base. The bottom center of the centrifugal chamber is connected to the motor and a plurality of through grooves are provided on the outer side of the bottom. A centrifugal film is arranged inside the centrifugal chamber.

[0016] In detail, the centrifugal chamber is composed of two parts, the upper part is a tubular structure, and the lower part is a hollow cone structure with a conical body bulging inward from the center.

[0017] In particular, the centrifugal membrane is water permeable, and the liquid can pass through the centrifugal membrane, thereby achieving the technical effect of separating the liquid and tumor tissue.

[0018] Furthermore, a discharge port is provided on the base, and the discharge port is communicated with a through groove provided on the outer side of the bottom of the centrifugal chamber.

[0019] Furthermore, the method for operating the device for recovering tumor tissue during intracranial glioma surgery comprises the following steps: S1: The air pump creates negative pressure in the liquid collection tank, and the glial tumor tissue is sucked through the suction tube; S2: Mix the collected glial tumor tissue with the red blood cell lysate in the collection tank; S3: The tissue mixture is centrifuged and filtered through a centrifuge to extract tumor tissue.

[0020] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention forms a negative pressure environment in the liquid collecting tank through an air pump, and then attracts the tumor tissue into the liquid collecting tank through a suction tube, and lyses the blood adhered to the surface of the tumor tissue through the red blood cell lysate stored in the liquid collecting tank. Furthermore, through the setting of a constant pressure chamber, the technical effect is achieved that the mixture of the red blood cell lysate and the tumor tissue in the liquid collecting tank can enter the centrifugal chamber through the through hole at the bottom of the liquid collecting tank for centrifugal filtration treatment without any residue.

[0021] 2. The present invention arranges an air vent pipe above the constant pressure chamber to avoid the problem of blockage at the connection between the liquid collecting tank and the constant pressure chamber, which causes excessive pressure and damage to the entire device. The gas squeezes the pressure relief ball, which in turn causes the spring to contract, and the gas is discharged through the opening on the wall of the air vent pipe, thereby achieving the technical effect of pressure relief.

[0022] 3. The present invention drives the centrifugal chamber to rotate by a motor, thereby realizing centrifugal movement of the mixture of red blood cell lysate and tumor tissue in the centrifugal chamber, so that the liquid in the mixture permeates through the centrifugal membrane and then enters the base through the through groove at the bottom of the centrifugal chamber, and is finally discharged through the discharge port, thereby realizing the recovery of tumor tissue and improving the purity and quality of the recovered tumor tissue.

[0023] 4. The present invention transports the cleaning liquid in the cleaning liquid tank to the liquid collecting tank through an air pump and a constant pressure cabin, thereby completing the technical effect of cleaning the liquid collecting tank and the centrifugal chamber, which helps to reduce the risk of cross infection and extend the service life of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 For the present invention Figure 1 The upward stereogram in Figure 3 It is a bottom view of the overall structure of the present invention; Figure 4 For the present invention Figure 3 Section view in the AA direction; Figure 5 For the present invention Figure 4 Enlarged view of part A in the middle; Figure 6 It is a three-dimensional diagram of the centrifugal mechanism structure of the present invention; Figure 7 For the present invention Figure 6 Middle top view; Figure 8 For the present invention Figure 7 Cross-sectional view in the middle BB direction; Fig. 9 It is a three-dimensional diagram of the base structure of the present invention; Fig.10 For the present invention Fig. 9 Middle bottom view; Fig.11 For the present invention Fig.10 Cross-sectional view in CC direction; In the figure: 1. base; 11. discharge port; 2. suction pipe; 3. centrifugal mechanism; 31. centrifugal chamber; 32. motor; 33. through slot; 34. centrifugal film; 4. negative pressure suction mechanism; 41. liquid collecting tank; 42. air pump; 43. constant pressure chamber; 44. solenoid valve core; 45. waterproof and breathable membrane; 46. delivery pipe; 47. baffle; 48. pull rod; 49. air release pipe; 410. spring; 411. pressure relief ball; 5. cleaning fluid tank. DETAILED DESCRIPTION

[0026] In order to enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0027] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "front end", "back end", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on the present invention.

[0028] In the description of this application, it should also be noted that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0029] The inventors have found that obtaining pathological tissue is the core work of making pathological diagnosis in the medical process. For most diseases, it is not too difficult as long as invasive or non-invasive professional means are adopted; however, for diseases with small lesions, difficult sampling, or the sampling process is likely to cause secondary damage to the patient, such as high-grade gliomas, myeloblastomas and other highly malignant tumors, due to the rich blood supply of the tumor, retaining a large amount of tissue at one time is likely to increase the amount of bleeding, and excessive pulling of brain tissue is likely to cause secondary damage. During the operation, many meaningful pathological tissues are directly sucked away by the suction device. The amount of tissue taken is sufficient to ensure pathological diagnosis, but it is very difficult to perform genetic testing or biological immunotherapy. Therefore, in clinical work, how to effectively recover pathological tissue is a difficult problem.

[0030] Based on the above findings, the present application provides a device for recovering tumor tissue during intracranial glioma surgery, comprising a base 1 and a suction tube 2, wherein a centrifugal mechanism 3 is rotatably sleeved on the base 1, a negative pressure suction mechanism 4 is rotatably connected above the centrifugal mechanism 3, and the negative pressure suction mechanism 4 is connected to the suction tube 2. Embodiment 1

[0031] Reference Figure 1-11 The present application provides a device for recovering tumor tissue during intracranial glioma surgery, comprising a base 1 and a suction tube 2, wherein a centrifugal mechanism 3 is rotatably sleeved on the base 1, a negative pressure suction mechanism 4 is rotatably connected above the centrifugal mechanism 3, and the negative pressure suction mechanism 4 is connected to the suction tube 2.

[0032] Specifically, the negative pressure suction mechanism 4 includes a liquid collecting tank 41, an air pump 42 and a constant pressure chamber 43; It should be noted that the liquid collecting tank 41 contains red blood cell lysis solution, which is used to lyse the blood adhering to the surface of the tumor tissue.

[0033] The liquid collecting tank 41 is connected to the suction pipe 2, and a solenoid valve core 44 is provided at the connection; Specifically, the provision of the electromagnetic valve core 44 can quickly form a negative pressure environment in the suction tube 2, thereby achieving the technical effect of quickly attracting tumor tissue.

[0034] The air pump 42 is disposed above the liquid collecting tank 41 and is connected to the suction pipe 2, and a waterproof and breathable membrane 45 is disposed at the connection; It should be noted that the provision of the waterproof breathable membrane 45 can prevent the tumor tissue in the suction tube 2 from entering the air pump 42. If the tumor tissue enters the air pump 42, it may cause blockage of the air pump 42 and contamination of the tumor tissue.

[0035] The constant pressure cabin 43 is disposed above the liquid collecting tank 41 , and a side end of the constant pressure cabin 43 is connected to the air pump 42 , and a bottom end of the constant pressure cabin 43 is connected to the liquid collecting tank 41 .

[0036] Furthermore, a through hole is formed at the bottom of the liquid collecting tank 41 and is connected to a delivery pipe 46 . A baffle 47 cooperating with the through hole is slidably provided on the side wall of the delivery pipe 46 . A pull rod 48 is connected to the end of the baffle 47 .

[0037] The through hole is closed and opened by pulling the pull rod 48 and then controlling the baffle 47. When the red blood cell lysate and the tumor tissue are mixed in the liquid collecting tank 41, the baffle 47 is used to cover the through hole, so that the red blood cell lysate and the tumor tissue are fully mixed in the liquid collecting tank 41, thereby improving the purity of the recovered tumor tissue.

[0038] Furthermore, the centrifugal mechanism 3 includes a centrifugal chamber 31 rotatably connected to the conveying tube 46 and a motor 32 fixedly connected to the base 1. The bottom center of the centrifugal chamber 31 is connected to the motor 32 and a plurality of through grooves 33 are opened on the outer side of the bottom. A centrifugal film 34 is arranged inside the centrifugal chamber 31.

[0039] Preferably, a discharge port 11 is provided on the base 1 , and the discharge port 11 is communicated with a through groove 33 provided on the outer side of the bottom of the centrifugal chamber 31 .

[0040] In the specific process, the liquid in the tumor tissue is filtered out through the centrifugal membrane 34 and then reaches the discharge port 11 through the through groove for discharge treatment. Embodiment 2

[0041] Based on Example 1, refer to Figure 4-5 As shown, in order to ensure the cleanliness and service life of the entire device, a cleaning liquid tank 5 and an air release pipe 49 are provided.

[0042] Specifically, a pressure relief pipe 49 is provided above the constant pressure cabin 43 , a spring 410 is provided inside the pressure relief pipe 49 , a pressure relief ball 411 is connected to the spring 410 , and the pressure relief ball 411 cooperates with an opening opened on the wall of the pressure relief pipe 49 .

[0043] When the connection between the liquid collecting tank 41 and the constant pressure chamber 43 is blocked and the pressure is too high, in order to avoid damage to the entire device, the pressure relief ball 411 is squeezed by gas, thereby contracting the spring 410, and the gas is discharged through the opening opened on the wall of the air relief pipe 49, thereby achieving the technical effect of pressure relief.

[0044] Furthermore, a cleaning liquid tank 5 is disposed on the air pump 42 , and the cleaning liquid tank 5 is communicated with the air pump 42 .

[0045] After the tumor tissue is collected, the cleaning liquid tank 5 is opened, so that the cleaning liquid in the cleaning liquid tank 5 enters the liquid collecting tank 41 through the air pump 42 and the constant pressure chamber 43 for cleaning, and the cleaned sewage reaches the discharge port 11 on the base 1 through the centrifugal chamber 31 for discharge and treatment. Specific application cases

[0046] Let's take high-grade glioma as an example. High-grade glioma is a tumor originating from glial cells in the brain. It accounts for 40% to 60% of all primary central nervous system tumors and is known as the most common primary intracranial tumor in adults. Common types include diffuse astrocytoma, oligodendroglioma, etc. The cause of the disease is not very clear, but it is usually related to environmental changes and gene mutations. Factors related to the pathogenesis of glioma include exposure to high doses of ionizing radiation and gene mutations associated with rare syndromes.

[0047] High-grade gliomas are characterized by low differentiation and high malignancy. High-grade gliomas grow faster, including grade 3 gliomas and grade 4 gliomas. If it is a grade 3 glioma, it is usually a gradual glioma, including poorly differentiated and dendritic gliomas. Patients with high-grade gliomas have a shorter survival time, a poor prognosis after treatment, and are not easy to completely remove. At present, it can only be treated by surgery, with timely radiotherapy and chemotherapy after surgery.

[0048] During surgery for intracranial high-grade glioma, it is usually necessary to collect tumor tissue for subsequent pathological diagnosis and molecular genetic testing. Based on this, the present application provides a device for recovering tumor tissue during intracranial glioma surgery. During specific use, a negative pressure environment is formed in the liquid collecting tank 41 through an air pump 42, and then the tumor tissue is attracted into the liquid collecting tank 41 through the suction tube 2, and the blood adhered to the surface of the tumor tissue is lysed by the red blood cell lysate stored in the liquid collecting tank 41. Further, through the setting of the constant pressure chamber 43, the mixture of the red blood cell lysate and the tumor tissue in the liquid collecting tank 41 can enter the centrifugal chamber 31 through the through hole at the bottom of the liquid collecting tank 41 for centrifugal filtration treatment without any residue.

[0049] Furthermore, the centrifugal chamber 31 is driven to rotate by the motor 32, so that the mixture of red blood cell lysate and tumor tissue is subjected to centrifugal motion in the centrifugal chamber 31, so that the liquid in the mixture permeates through the centrifugal membrane 34 and then enters the base 1 through the through groove 33 at the bottom of the centrifugal chamber 31, and is finally discharged through the discharge port 11, thereby realizing the recovery of tumor tissue and improving the purity and quality of the recovered tumor tissue.

[0050] After the tumor tissue is recovered, the cleaning liquid in the cleaning liquid tank 5 is transported to the liquid collecting tank 41 through the air pump 42 and the constant pressure chamber 43, thereby completing the cleaning process of the liquid collecting tank 41 and the centrifugal chamber 31.

[0051] It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for recovering tumor tissue during intracranial glioma surgery, comprising a base (1) and a suction tube (2), characterized in that: A centrifugal mechanism (3) is rotatably sleeved on the base (1), a negative pressure suction mechanism (4) is rotatably connected above the centrifugal mechanism (3), and the negative pressure suction mechanism (4) is connected to the suction tube (2).

2. The device for recovering tumor tissue during intracranial glioma surgery according to claim 1, characterized in that: The negative pressure suction mechanism (4) comprises a liquid collecting tank (41), an air pump (42) and a constant pressure chamber (43); The liquid collecting tank (41) is connected to the suction pipe (2), and a solenoid valve core (44) is provided at the connection; The air pump (42) is arranged above the liquid collecting tank (41) and is connected to the suction tube (2), and a waterproof and breathable membrane (45) is provided at the connection; The constant pressure cabin (43) is arranged above the liquid collecting tank (41), and the side end of the constant pressure cabin (43) is connected to the air pump (42), and the bottom end is connected to the liquid collecting tank (41).

3. The device for recovering tumor tissue during intracranial glioma surgery according to claim 2, characterized in that: A cleaning liquid tank (5) is provided on the air pump (42), and the cleaning liquid tank (5) is in communication with the air pump (42).

4. The device for recovering tumor tissue during intracranial glioma surgery according to claim 3, characterized in that: The bottom of the liquid collecting tank (41) is provided with a through hole and is connected to a delivery pipe (46). A baffle (47) cooperating with the through hole is slidably provided on the side wall of the delivery pipe (46). The end of the baffle (47) is connected to a pull rod (48).

5. The device for recovering tumor tissue during intracranial glioma surgery according to claim 4, characterized in that: A pressure relief pipe (49) is arranged above the constant pressure cabin (43), a spring (410) is arranged inside the pressure relief pipe (49), a pressure relief ball (411) is connected to the spring (410), and the pressure relief ball (411) cooperates with an opening formed on the wall of the pressure relief pipe (49).

6. The device for recovering tumor tissue during intracranial glioma surgery according to claim 5, characterized in that: The centrifugal mechanism (3) comprises a centrifugal chamber (31) rotatably connected to a delivery pipe (46) and a motor (32) fixedly connected to the base (1); the center of the bottom of the centrifugal chamber (31) is connected to the motor (32) and a plurality of through slots (33) are provided on the outer side of the bottom; and a centrifugal film (34) is provided inside the centrifugal chamber (31).

7. The device for recovering tumor tissue during intracranial glioma surgery according to claim 6, characterized in that: The base (1) is provided with a discharge port (11), and the discharge port (11) is communicated with a through groove (33) provided on the outer side of the bottom of the centrifugal chamber (31).

8. The method for operating the device for recovering tumor tissue during intracranial glioma surgery according to claim 2, characterized in that: The following steps are involved: S1: The air pump (42) creates negative pressure in the liquid collection tank (41), and the glial tumor tissue is sucked through the suction tube (2); S2: Mixing the collected glial tumor tissue with the red blood cell lysate in the collection tank (41); S3: The tissue mixture is centrifuged and filtered through a centrifugal mechanism (3) to extract tumor tissue.

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