Preparation method of human hair hydrophilic modified wound dressing biological material

Abstract

The invention discloses a method for preparing a wound dressing from human hair, which comprises the following steps: firstly, carrying out hydrophilic modification on the human hair to enable the human hair to easily absorb water and be easily biodegraded, then pouring the human hair into a mold for drying or freeze-drying to obtain a membrane or porous sponge with a required shape and size, and finally, preparing nano-silver particles by adopting an in-situ method in an adhesion manner. The prepared wound dressing is good in biocompatibility, capable of stopping bleeding quickly and antibacterial. The human hair keratin is free of immunogenicity, rich in raw material source, low in cost, capable of being industrially produced and applied on a large scale, good in cell compatibility and capable of stopping bleeding, and a method for preparing nano-silver in situ through an adhesion method is simple. By means of the method, an ideal wound dressing can be obtained. The human hair keratin porous sponge and film prepared by the method provided by the invention adsorb wound blood through the porous structure to form a blood coagulation net rack, can quickly stop bleeding, has the characteristics of no antigenicity, good biocompatibility, degradability, stimulation of cell division and promotion of healing, and belongs to the porous sponge and film which can be implanted into a human body and can be used for quickly stopping bleeding. The biological scaffold material has the advantages of good biocompatibility, good histocompatibility, capability of inducing the formation of autologous tissues, and capability of being degraded and absorbed in the body, and has obvious progress compared with the existing medical material.

Description

Technical Field

[0001] The present invention relates to a method for preparing a human hair keratin medical biomaterial, and in particular to a method for preparing a biomaterial using human hair keratin to produce porous sponges, powders, gels, catheters or films. Background Art

[0002] Finding ideal medical absorbable biomaterials has always been the focus of scientists' research. Such biomaterials are required to have biocompatibility, plasticity and stability to meet various medical requirements, including structural materials of hemostatic sponges and artificial skin, and wound healing dressings.

[0003] At present, the medical absorbable materials used in clinical practice mainly include two types: natural absorbable materials and artificially synthesized absorbable materials. The former are mainly prepared with sheep intestine, collagen, chitin (chitosan), etc., and the latter are mainly composed of polylactic acid, carboxymethyl fiber, etc. The physical properties of the above products determine that when they come into contact with blood, they will induce a series of non-specific protein adsorption, and have the risk of causing a series of non-biocompatibility reactions such as infection and tissue rejection.

[0004] The ideal dressing should first meet the following functions and characteristics: ① protect the wound; ② maintain the moisture of the wound surface; ③ allow water to pass through; ④ antibacterial; ⑤ release healing factors to the wound; ⑥ easy to use; ⑦ no need to change frequently; ⑧ non-toxic; ⑨ non-antigenic. It is conducive to wound cell regeneration and repair cell growth; has a strong ability to absorb wound exudate, so that the wound surface can be fully drained; can maintain wound moisture; prevent bacterial contamination; reduce the loss of nutrients in the wound surface; enhance blood circulation and microcirculation in the wound surface; protect the new tissue in the wound surface; and has anti-inflammatory and analgesic functions.

[0005] Human hair keratin is biodegradable, has high biological activity, good compatibility, and low immune rejection. Compared with other materials, its source is almost unlimited, so its research and application in the field of regenerative medicine has received more and more attention. In recent years, human hair keratin has been used in the form of gels, sponge scaffolds, coatings, membranes, and composite materials in nerve materials, skin dressings, cell culture, drug carriers, and soft tissue and tendon repair. Human hair keratin can promote peripheral nerve regeneration and is an ideal material for bridging peripheral nerve defects. It shows its development potential in the field of regenerative medicine. Keratin is the main structural protein of various types of epithelial cells and plays an important role in wound healing. Keraplast in the United States has made human hair keratin into skin wound covering materials and commercialized them, which will have a positive impact on the treatment of full-thickness skin defects and non-full-thickness skin defects.

[0006] Application No. CN201210073183.0 A method for preparing human serum albumin medical biomaterials, using human serum albumin as raw material, the source of which is limited, and it cannot be mass-produced, and the cost is very high.

[0007] Application No. CN201510233593.0 A preparation method of a human hair keratin composite wound dressing discloses a composite method for preparing a wound dressing using human hair keratin. 1 gram of human hair is washed, dried, cut into small pieces, swelled in a 50-gram mixed solution of thioethanol (or thioacetic acid), sodium dodecyl sulfate and urea (amount ratio of 1:1:10) for 1 to 2 hours, stirred at 60 degrees Celsius for 48 hours, filtered to obtain a keratin solution, and dialyzed for 2-3 days. Then, a modifier iodoacetic acid is added to the keratin solution, reacted at room temperature in the dark for 12 hours, dialyzed for 2 days, and freeze-dried to obtain a keratin sponge. Keratin and biodegradable chemical polymers are then composite-spun to prepare a keratin composite membrane, and finally nanosilver particles are attached and prepared by an in-situ method to obtain a wound dressing. The process equipment requirements are high, the time is long, the investment is high, the cost is high, and it is not environmentally friendly. A large amount of urea is needed, and dialysis bags are used to remove the urea. The dialysis time is long, taking 72 hours, and a large amount of flowing water is required for dialysis. It is not environmentally friendly, the production volume is small, and it cannot be produced on a large scale. Summary of the invention

[0008] In order to solve the above technical problems, the purpose of the present invention is to provide a method for preparing a human hair wound dressing medical biomaterial with low energy consumption, low cost, small investment and large-scale industrial production.

[0009] In order to achieve the above-mentioned invention object, the present invention adopts the following technical scheme:

[0010] The following steps are involved:

[0011] Step 1) Pretreatment of human hair: Wash the human hair, remove impurities, and dry it for later use;

[0012] Step 2) Modification of human hair keratin: add 1-50 g of sodium tetrathionate and 1 g of sodium sulfite to 1 liter of hot water. Then add 1-50 g of human hair, keep warm at 20 to 100 degrees, filter out the human hair fiber after 0.5 to 24 hours, wash it repeatedly with water, and obtain a gel-like brown human hair fiber;

[0013] Step 3) Drying: Pour the mixture into a stainless steel mold and dry or freeze-dry it to obtain a membrane or porous sponge of the desired shape and size. A small amount of glycerol may be added to maintain the flexibility of the membrane.

[0014] Step 4) preparing a composite film with attached nano-silver particles by in-situ reduction method: injecting the human hair keratin film in the mold into a 0.01%-5% mass percentage concentration of silver nitrate or silver sulfate ammonia solution for 0.1-2 hours, and drying again. The silver ions attached to the surface of the human hair film are in-situ reduced on the surface of the human hair keratin rich in reducing groups to obtain a nano-silver composite film.

[0015] Step 5) The dried film obtained in step 3) is crushed into powder by a pulverizer, passed through a 50-mesh sieve, and the powder is added with 1-10 times of water to obtain an injectable human hair keratin gel, which can be used for tissue filling. By controlling the proportion of the hydrophilic modifier, the time of degradation and absorption in the body can be changed.

[0016] Human hair keratin sponge absorbs blood from the wound through its porous structure to form a coagulation grid, seal blood vessel ruptures or wounds, and activate platelets to achieve the purpose of hemostasis. It can adhere to the wound very firmly, has good hemostatic effect, is safe and convenient to use, and is non-antigenic, biocompatible, degradable, stimulates cell division, and promotes healing. It has good biocompatibility. Moreover, the material has excellent hydrophilicity and has a strong ability to absorb water. When applied to the wound on the body surface, the wound is dry, not prone to infection, and the wound heals quickly. Used for hemostasis of internal organs, the material is degradable and absorbent, so it is a significant improvement over existing medical materials.

[0017] Beneficial effects:

[0018] The method for preparing the human hair keratin biomaterial of the invention has simple process, small reagent dosage, low cost, environmental friendliness, stable structure and can be industrially produced on a large scale. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with specific implementation methods. The following implementation methods are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0020] Embodiment 1:

[0021] Step 1) Pretreatment of human hair: Wash the human hair, remove impurities, and dry it for later use;

[0022] Step 2) Modification of human hair keratin: Add 5 grams of sodium tetrathionate and 5 grams of sodium sulfite to 1 liter of hot water. Then add 50 grams of human hair. Keep the temperature at 50 degrees. After 24 hours, filter out the human hair fiber and wash it repeatedly with water to obtain a gel-like brown human hair fiber;

[0023] Step 3) Drying: Pour the mixture into a mold and dry or freeze-dry it to obtain a membrane or porous sponge of the desired shape and size. To maintain the flexibility of the membrane, 2% glycerol may be added;

[0024] Step 4) Preparation of a composite film with attached nano-silver particles by in-situ reduction method: injecting the same volume of 0.2% silver nitrate ammonia solution into the human hair keratin film in the mold for 0.5 hours, and drying again. The silver ions attached to the surface of the human hair film are in-situ reduced on the surface of the human hair keratin rich in reducing groups to obtain a nano-silver composite film.

[0025] Embodiment 2:

[0026] Step 1) Pretreatment of human hair: Wash the human hair, remove impurities, and dry it for later use;

[0027] Step 2) Modification of human hair keratin: Add 25 grams of sodium tetrathionate and 25 grams of sodium sulfite to 1 liter of hot water. Then add 50 grams of human hair. Keep the temperature at 65 degrees. After 12 hours, filter out the human hair fiber and wash it repeatedly with pure water without using a dialysis bag for desalination to obtain a gel-like brown human hair fiber;

[0028] Step 3) Drying: Pour the mixture into a mold and dry or freeze-dry it to obtain a membrane or porous sponge of the desired shape and size. To maintain the flexibility of the membrane, 5% glycerol may be added;

[0029] Step 4) Preparation of a composite film with attached nano-silver particles by in-situ reduction method: injecting the same volume of 0.5% by mass concentration of silver nitrate ammonia solution into the human hair keratin film in the mold for 1 hour, and drying again. The silver ions attached to the surface of the human hair film are in-situ reduced on the surface of the human hair keratin rich in reducing groups to obtain a nano-silver composite film.

Claims

1. A method for preparing a hydrophilic modified human hair wound dressing biomaterial, characterized in that The method comprises the following steps: Step 1) Pretreatment of human hair: Wash the human hair, remove impurities, and dry it for later use; Step 2) Modification of human hair keratin: Add an appropriate amount of hydrophilic modifier to the human hair obtained in step 1), which is a mixture of sodium tetrathionate and sodium sulfite. Add 1 to 50 grams of sodium tetrathionate and sodium sulfite to 1 liter of hot water. Then add 1 to 50 grams of human hair. Keep the temperature at 20 to 100 degrees. After 1 to 24 hours, filter out the human hair fiber and repeatedly rinse with water to obtain a gel-like brown human hair fiber; Step 3) Drying: Pour the mixture into a mold and dry or freeze-dry it to obtain a membrane or porous sponge of the desired shape and size. To prepare the membrane, 0.5%-10% glycerol may be added to maintain the flexibility of the membrane. Step 4) preparing a composite film with attached nano-silver particles by in-situ reduction method: injecting the human hair keratin film in the mold into a silver nitrate ammonia solution with the same volume and a mass percentage concentration of 0.01%-5% for 0.1-2 hours, and drying again, the silver ions attached to the surface of the human hair film are in-situ reduced on the surface of the human hair keratin rich in reducing groups to obtain a nano-silver composite film. Step 5) The dried film obtained in step 3) is crushed into powder by a pulverizer, passed through a 50-mesh sieve, and 1-10 times of water is added to the obtained powder to obtain an injectable human hair keratin gel, which can be used for tissue filling.