Composition with soothing and repairing effects and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with soothing and repairing effects and application of the composition in cosmetics. Specifically, the composition is prepared from the following components in parts by mass: 0.01 to 1 part of glycosylglycerol, 0.005 to 0.2 part of hyaluronic acid disaccharide and 1 to 12 parts of sea-buckthorn extract. The soothing and repairing composition is prepared by compounding glycosylglycerol, hyaluronan disaccharide and a sea-buckthorn extract, and tests prove that the composition has remarkable effects of synergistically inhibiting mast cell degranulation and promoting generation of barrier related factors DSG1, DSC1 and Ki67, can be used in cosmetics to improve the skin sensitivity problem, and is good in stability, free of toxic and side effects and free of side effects. Therefore, the method has good practical application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition with soothing and repairing effects and its application in cosmetics. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] The incidence of sensitive skin in the female population is relatively high. Data show that on average one in three women has sensitive skin, among which people aged 20 to 35 are the most susceptible group, accounting for more than 70%. Sensitive skin often manifests as itching, tingling and burning of the skin, which is related to impaired skin barrier function and skin neuroimmune disorders. Inflammatory reactions mediated by immune cells are an important factor in causing sensitive skin. Mast cells are immune cells that trigger allergic reactions in organisms and participate in the host's immune defense. When the body comes into contact with allergic substances, mast cells release substances in the granules to the outside of the cells. This process is also called "mast cell degranulation". After mast cell degranulation, inflammatory mediators such as histamine and leukotrienes are released, causing allergic reactions such as itching. Inhibiting mast cell degranulation can effectively relieve sensitive phenomena such as skin itching.

[0004] The skin barrier is composed of the "brick wall structure" of the stratum corneum and the tight connection of the stratum granulosum. It is an important basis for the skin to perform its physiological functions. Barrier damage is directly related to the appearance of sensitive skin. The causes and mechanisms of barrier damage are very complex. Among them, abnormalities in corneous desmosomes can easily lead to excessive desquamation of keratinocytes, which may lead to skin barrier defects and subsequent skin inflammation. Desmosomes are the main structure connecting keratinocytes, which are composed of oval dense thickening of adjacent cell membranes. Desmoglein 1 (DSG1) and desmocollin 1 (DSC1) are the main proteins that make up desmosomes. DSC1 and DSG1 form the electron-transparent intercellular space and intercellular contact layer of desmosomes. When cells undergo cascade reactions, they will cause changes in the desmosome structure, resulting in loss of intercellular adhesion. The normal expression of desmosomal protein structure and the coordination of their functions can promote the integrity of the epidermal structure and the restoration of barrier function.

[0005] Although there are many soothing and repairing products that aim to solve the problem of skin sensitivity, these products often have only a single function and minimal effect. Sensitivity often recurs after discontinuation of use. Even if multiple ingredients are stacked in the product or the amount of ingredients added is increased, the expected effect cannot be achieved. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a composition with soothing and repairing effects and its application. The soothing and repairing composition is compounded with glycerol glucoside, hyaluronic acid disaccharide, and sea buckthorn extract. It has been verified by experiments that the composition has significant synergistic effects of inhibiting mast cell degranulation and promoting the generation of barrier-related factors DSG1, DSC1, and Ki67. It can be used in cosmetics to improve skin sensitivity problems and has good stability. Based on the above research results, the present invention is completed.

[0007] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0008] The first aspect of the present invention provides a composition with soothing and repairing effects, the composition comprising the following components in parts by weight:

[0009] 0.01-1 part of glyceryl glucoside, 0.005-0.2 part of hyaluronic acid disaccharide, 1-12 parts of sea buckthorn extract.

[0010] The seabuckthorn extract can be obtained by any of the following extraction and preparation methods:

[0011] Extraction method 1 is as follows:

[0012] S1-1, mixing the crushed seabuckthorn fruit with water and subjecting the mixture to microwave extraction, subjecting the obtained mixture to vacuum decompression reflux extraction, and filtering the extract to obtain a filtrate;

[0013] S1-2, the filtrate obtained in step S1 is concentrated under reduced pressure, ethanol is added, and the mixture is allowed to stand at low temperature. After filtering, the filtrate is concentrated under reduced pressure for a second time, 1,3-butanediol is added, and the mixture is stirred and mixed evenly. After filtering, the seabuckthorn extract is obtained.

[0014] Extraction method 2 is as follows:

[0015] S2-1, taking the crushed seabuckthorn fruit, adding ethanol aqueous solution to perform reflux extraction, filtering the filtrate, repeatedly extracting the residue 1-3 times, and combining the filtrate;

[0016] S2-2, the filtrate obtained in step S1 is concentrated under reduced pressure, 1,3-butanediol is added thereto, the mixture is stirred and mixed evenly, and the sea buckthorn extract is obtained after filtering.

[0017] The second aspect of the present invention provides the use of the above composition in soothing, repairing and skin care products.

[0018] The third aspect of the present invention provides a soothing, repairing and skin-care cosmetic, which comprises at least the above-mentioned composition; the cosmetic may be a skin-care cosmetic.

[0019] The fourth aspect of the present invention provides a method for preparing the soothing and repairing skin care cosmetics, the method comprising the step of mixing the composition and other raw material components.

[0020] Beneficial technical effects of one or more of the above technical solutions:

[0021] (1) The soothing and repairing composition obtained by the above technical solution has a significant synergistic effect. The soothing and repairing composition obtained by the above technical solution has a significant synergistic effect, which achieves a good soothing and repairing effect by inhibiting mast cell degranulation and promoting the generation of barrier-related factors DSG1, DSC1, and Ki67;

[0022] (2) It has been verified through experiments that seabuckthorn extract ① has better skin care effects than seabuckthorn extract ②, thus making the product more effective.

[0023] (3) Experimental results show that hyaluronic acid disaccharide (400 Da) has better synergistic effect than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da), glycerol glucoside and sea buckthorn extract.

[0024] (4) Seabuckthorn extract is prone to color change, which affects the stability of the product. The stability of the product can be significantly improved by combining it with hyaluronic acid disaccharide (400Da) and glycerol glucoside.

[0025] (5) The preparation method of the soothing and repairing skin care cosmetics obtained by the above technical scheme is simple and easy, cost-saving, and the raw materials are easy to obtain. It is suitable for mass production. It has passed the human efficacy experiment test and has good soothing and repairing effects. Therefore, it has good practical application value.

[0026] At the same time, the cosmetic preparation method obtained by the above technical solution is simple and easy, the raw materials are easy to obtain, and it is suitable for mass production, so it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the drawings required for use in the description of the embodiment will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0028] Figure 1 : Bar graph of DSC1 immunofluorescence results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0029] Figure 2: Bar graph of DSG1 immunofluorescence results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0030] Figure 3 : Bar graph of Ki67 immunohistochemistry results (## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control).

[0031] Figure 4 : Pictures of changes in facial red area before and after using the sample. DETAILED DESCRIPTION

[0032] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0033] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0034] In a typical embodiment of the present invention, a composition with soothing and repairing effects is provided, wherein the composition comprises the following components in parts by weight:

[0035] 0.01-1 part of glyceryl glucoside, 0.005-0.2 part of hyaluronic acid disaccharide, 1-12 parts of sea buckthorn extract.

[0036] In the present invention, the glycerol glucoside is specifically 2-α-glycerol glucoside (2-O-α-D-glucopyranosyl-sn-glycerol, 2-αGG), which has antioxidant activity and high moisturizing ability.

[0037] In the present invention, the hyaluronic acid disaccharide has an INCI name of hydrolyzed sodium hyaluronate and a molecular weight of 400 Da.

[0038] The seabuckthorn extract can be obtained by any of the following extraction and preparation methods; specifically,

[0039] Extraction method 1 is as follows:

[0040] S1-1, mixing the crushed seabuckthorn fruit with water and subjecting the mixture to microwave extraction, subjecting the obtained mixture to vacuum decompression reflux extraction, and filtering the extract to obtain a filtrate;

[0041] S1-2, the filtrate obtained in step S1 is concentrated under reduced pressure, ethanol is added, and the mixture is allowed to stand at low temperature. After filtering, the filtrate is concentrated under reduced pressure for a second time, 1,3-butanediol is added, and the mixture is stirred and mixed evenly. After filtering, the seabuckthorn extract is obtained.

[0042] Wherein, in the step S1-1, the mass ratio of the crushed sea buckthorn fruit to water is 1:15 to 1:20; the microwave power is 300 to 400 W, and the microwave treatment time is 20 to 30 min; the extraction temperature of the vacuum decompression reflux extraction is 45-60°C, the extraction time is 0.5-2 h, and the number of reflux extractions can be 1 to 3 times. When the number of reflux extractions is greater than 1 time, the extracts obtained each time are combined and filtered.

[0043] In the step S1-2, the reduced pressure concentration temperature is controlled at 50-70°C, and the reduced pressure concentration is performed to 6-10 times the mass of the crushed sea buckthorn fruit in step S1; ethanol is added until the alcohol content in the obtained concentrated solution is 60-80%; the solution is placed at 4±2°C and allowed to stand for 10-30 hours; the reduced pressure concentration is performed again to 2-6 times the mass of the crushed sea buckthorn fruit in step S1, and then 1-1.5 times the mass of 1,3-butanediol is added to the obtained secondary concentrated solution.

[0044] The obtained seabuckthorn extract has a concentration of 0.10-0.12 g / g based on the crude drug amount.

[0045] Extraction method 2 is as follows:

[0046] S2-1, taking the crushed seabuckthorn fruit, adding ethanol aqueous solution to perform reflux extraction, filtering the filtrate, repeatedly extracting the residue 1-3 times, and combining the filtrate;

[0047] S2-2, the filtrate obtained in step S1 is concentrated under reduced pressure, 1,3-butanediol is added thereto, the mixture is stirred and mixed evenly, and the sea buckthorn extract is obtained after filtering.

[0048] Wherein, in the step S2-1, the ethanol concentration in the ethanol aqueous solution is 20-40%, the mass ratio of the crushed seabuckthorn fruit to the ethanol aqueous solution is 1:8-1:12, and the specific conditions of the reflux extraction are: reflux extraction at 60-80° C. for 0.2-1 h;

[0049] In the step S2-2, the reduced pressure concentration temperature is controlled at 50-70° C., and the reduced pressure concentration is performed to 2-6 times the mass of the crushed sea buckthorn fruit in step S1; then 1,3-butanediol is added in an amount of 1-1.5 times the mass of the concentrate.

[0050] The obtained seabuckthorn extract has a concentration of 0.10-0.12 g / g based on the crude drug amount.

[0051] In another specific embodiment of the present invention, the use of the above composition in products for soothing, repairing and skin care is provided. The present invention has verified through experiments that the above composition can achieve a soothing effect by inhibiting mast cell degranulation; promote the generation of barrier-related factors DSG1, DSC1, and Ki67 to achieve a repairing effect; and is safe and has no toxic side effects, so it can be applied to cosmetics for soothing, repairing and skin care. Therefore, the product can be a cosmetic, and further can be a skin care cosmetic (i.e., skin care product). It should be noted that in the present invention, the cosmetic refers to a daily chemical industrial product that is spread on any part of the human body surface, especially on the skin, by rubbing, spraying or other similar methods.

[0052] In another specific embodiment of the present invention, a soothing, repairing and skin-care cosmetic is provided, wherein the cosmetic comprises at least the above-mentioned composition; the cosmetic may be a skin-care cosmetic.

[0053] The cosmetics may also contain any other raw material ingredients permitted to be added in the cosmetics field, including but not limited to emulsifiers, emollients, humectants and thickeners.

[0054] At the same time, the present invention can also be used to prepare different cosmetic dosage forms, such as liquid, gel, ointment, cream, lotion, spray, facial mask, etc., by reasonably adding the above raw materials and ingredients, which are not specifically limited here.

[0055] In another specific embodiment of the present invention, based on the total mass of the soothing and repairing skin care cosmetic, the soothing and repairing composition is included in an amount of 0.5-10.0%.

[0056] In another specific embodiment of the present invention, based on the total mass of the soothing and repairing skin care cosmetic, the soothing and repairing composition is included in an amount of 2.0-5.0%.

[0057] In another specific embodiment of the present invention, a method for preparing the above-mentioned soothing and repairing skin care cosmetics is provided, and the preparation method comprises the step of mixing the composition and other raw material components.

[0058] The present invention is further described below in conjunction with examples. The present invention is further described below by way of example, but the present invention is not limited to the scope of the embodiments described. Based on the embodiments in the present invention, any changes to the present invention by those skilled in the art without making a creative premise belong to the scope of protection of the present invention. The hyaluronic acid disaccharide used in the embodiments and comparative examples is named Huanling 400, the INCI name is hydrolyzed sodium hyaluronate, the molecular weight is 400Da, and it is purchased from Shandong Baifu Furuida Pharmaceutical Co., Ltd.; glycerol glucoside is specifically 2-α-glycerol glucoside, purchased from Qingdao Zhongke Lanzhi Biotechnology Development Co., Ltd.; other reagents and materials are all ordinary commercially available products unless otherwise specified.

[0059] Examples 1-4 and Comparative Examples 1-9:

[0060] The specific scheme is shown in Table 1 below, calculated by weight. The preparation method is as follows: glycerol glucoside, hyaluronic acid disaccharide, and seabuckthorn extract are mixed together and dissolved in purified water.

[0061] The preparation method of the seabuckthorn extract ① comprises the following specific steps:

[0062] (1) Take an appropriate amount of dried seabuckthorn fruit powder and mix it with water for microwave extraction, the material-liquid mass ratio is 1:20, the microwave power is 350 W, the microwave time is 30 min, the treated mixed solution is subjected to vacuum decompression reflux extraction, the extraction temperature is 50°C, the extraction time is 60 min, the reflux extraction is repeated twice, and the two extracts are combined and filtered to obtain the product.

[0063] (2) The filtrate obtained in step (1) is concentrated under reduced pressure at 60°C to 8 times the mass of seabuckthorn fruit powder, ethanol is added until the alcohol content of the concentrate is 70%, and the concentrate is placed in a 4°C refrigerator for 24 hours, filtered, and the filtrate is concentrated under reduced pressure to about 4.5 times the mass of seabuckthorn fruit powder, 1,3-butanediol is added in an amount of about 1.1 times the mass of the concentrate, and the mixture is stirred and mixed uniformly. After filtering, seabuckthorn extract ① is obtained. The concentration is 0.1021 g / g based on the crude drug amount.

[0064] The preparation method of the seabuckthorn extract ② comprises the following specific steps:

[0065] (1) Take an appropriate amount of dried seabuckthorn fruit powder, add 40% ethanol aqueous solution, the material-liquid mass ratio is 1:10, reflux extraction at 60°C for 40 minutes, filter the filtrate, repeat the extraction of the filter residue twice, and combine the three filtrates.

[0066] (2) The filtrate obtained in step (1) was concentrated under reduced pressure at 60° C. to a concentration of about 4.5 times the mass of seabuckthorn fruit powder, and 1,3-butanediol was added in an amount of about 1.1 times the mass of the concentrate, and the mixture was stirred and mixed evenly. After filtering, seabuckthorn extract ② was obtained. The concentration of the extract was 0.1053 g / g based on the crude drug amount.

[0067] Table 1 Specific examples and comparative examples ingredient list

[0068]

[0069]

[0070] Examples 5, 6, and Comparative Examples 10-16: Essence Water

[0071] Preparation method of essence water:

[0072] S1: Add the raw materials in phase A respectively, heat to 85℃ and stir to dissolve;

[0073] S2: After the dissolution is complete, start cooling down. When the temperature drops to 45°C, add the ingredients in phase B respectively, stir to dissolve and disperse evenly;

[0074] S3: When the temperature drops to 40°C, add the ingredients of phase C in sequence, stir to dissolve and disperse evenly, and the essence water product is obtained.

[0075] Table 2 Essence water product formula

[0076]

[0077] Note: PHL is 1,3-propylene glycol, 1,2-hexanediol, and caprylhydroxamic acid, purchased from Jingchenlong Chemical Co., Ltd.

[0078] Phase B in the formulations of Examples 5 and 6 and Comparative Examples 10 to 16 was added according to Table 3.

[0079] Table 3 Summary of raw materials for phase B of Example 6 and Comparative Examples 10 to 16

[0080]

[0081]

[0082] Test Example 1: Stability Test

[0083] Examples 5, 6 and comparative examples 10-16 were subjected to stability tests. Examples 5, 6 and comparative examples 10-16 were placed under hot and cold cycle (-18°C, 4°C, 45°C, cycled every 3 days) conditions for 4 weeks and 45°C and light conditions for 12 weeks, respectively, and observed every two weeks. During the observation, the samples needed to be cooled to room temperature to check whether the samples changed color.

[0084] Table 4 Stability test results

[0085]

[0086] The test results are shown in Table 4. Examples 5 and 6 did not change color under hot and cold cycles, 45°C and light conditions. Comparative Examples 10, 11, 13-16 all changed color under hot and cold cycles, 45°C and light conditions. Comparative Example 12 changed color under 45°C and light conditions. The results show that the seabuckthorn extract ① prepared by method 1 and the seabuckthorn extract ② prepared by method 2 are prone to color change under 45°C and light conditions. According to the color change time, the seabuckthorn extract ① is more stable than the seabuckthorn extract ②. Glycerol glucoside and hyaluronic acid disaccharide can significantly improve the color change of the seabuckthorn extract when combined with the seabuckthorn extract ① and the seabuckthorn extract ②, but the color change still occurred during the test time when combined with the seabuckthorn extract ②, and the stability test was not passed. When combined with the seabuckthorn extract ①, no color change occurred during the test time, and the stability test was passed.

[0087] Test Example 2: In vitro soothing efficacy study

[0088] In this experiment, mast cells were used as experimental subjects. A degranulation model was established by stimulating mast cells with C48 / 80. The soothing effect of the test samples was evaluated based on the morphology and degranulation rate.

[0089] Press 1×10 5 Inoculate cells into 24-well plates at a seeding density of 100 cells / well and incubate overnight in an incubator (37°C, 5% CO2). Prepare the test substance working solution according to the test scheme in Table 5. When the cell plating rate in the 24-well plate reaches 40% to 50%, group administration is performed. Add 1 mL of culture medium containing the sample to be tested at different concentrations to each well, and set up 3 replicates for each group. After the administration is completed, place the 24-well plate in an incubator (37°C, 5% CO2) and culture for 2 hours.

[0090] After the samples were incubated for 2 hours, the solution was discarded; according to the experimental grouping, the blank control group was added with 1 mL of serum-free high-glucose DMEM culture medium, the negative control group was added with 1 mL of serum-free high-glucose DMEM culture medium containing C48 / 80, the positive control group was added with 1 mL of serum-free high-glucose DMEM culture medium containing C48 / 80 and sodium cromoglycate, and the sample group was added with 1 mL of serum-free high-glucose DMEM culture medium containing C48 / 80 and the corresponding sample administration concentration. After the end of the administration, C48 / 80 was stimulated for 45 minutes, and the reaction was terminated by ice bath. The inhibition rate calculation formula = (OD value of the NC group - OD value of the sample) / OD value of the NC group * 100%.

[0091] After the culture was completed, the degranulation of cells in each group was observed under an inverted microscope, and the degranulation rate of cells was counted and calculated using IPP software.

[0092] Table 5 Test grouping

[0093]

[0094]

[0095] The test results are shown in Table 6. Compared with the unstimulated group (BC group), after C48 / 80 stimulation (NC group), the cells showed obvious degranulation morphology (P < 0.01). After adding sodium cromoglycate treatment (PC group), the number of degranulated cells in the visual field decreased significantly (P < 0.01), indicating that the C48 / 80-induced mast cell degranulation model was successfully established and the experiment was effective. Compared with the NC group, the number of degranulated cells in the visual field of Examples 1-4 and Comparative Examples 1-9 decreased significantly, showing a significant inhibitory effect on mast cell degranulation. The inhibitory effects of Examples 2 and 3 were the most significant, and the inhibition rates of the Examples were higher than those of the Comparative Examples, indicating that the synergistic effect of the seabuckthorn extract extracted by method 1 was more significant; hyaluronic acid disaccharide was more significant than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da) and glycerol glucoside, and seabuckthorn extract; the soothing repair composition can synergistically inhibit mast cell degranulation, thereby achieving a soothing effect.

[0096] Table 6 Summary of mast cell degranulation rate

[0097]

[0098] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control.)

[0099] Experimental Example 3: In vitro repair efficacy study

[0100] This test uses a 3D epidermal skin model For the test model, 0.1% SLS working solution was prepared by taking 1 mL of 0.2% SLS solution and adding 1 mL of PBS to prepare 0.1% SLS working solution; Positive control group (WY14643) working solution was prepared by taking 10 μL of 30 mM WY14643 mother solution and dissolving it in 6 mL of culture medium to prepare 50 μM WY14643.

[0101] According to the test grouping in Table 7, the model was transferred to a 6-well plate (0.9 mL of EpiGrowth culture medium was added in advance), and the test group number was marked on the 6-well plate. 25 μL of 0.1% SLS solution was added to the surface of the negative control group, the positive control group, Examples 1-4, and Comparative Examples 1-6, and incubated for 30 minutes. After the incubation, the positive control group added a working solution of the corresponding concentration, 12.5 μL of the sample to be tested was added to the surface of the model, and after evenly coating, it was placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 hours. After the incubation, the test substance remaining on the surface of the model was cleaned with a sterile PBS solution, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab.

[0102] Immunofluorescence test: Take the model for detection, cut off the model, fix it with 4% paraformaldehyde, and after fixation for 24 hours, perform immunofluorescence detection of DSC1 and DSG1 content, observe under a microscope, collect pictures and analyze them.

[0103] Immunohistochemical test: Take the model for detection, remove it by circumcision, fix it with 4% paraformaldehyde, and after fixation for 24 hours, perform immunohistochemical detection of Ki67 content, take pictures under a microscope, and collect and analyze the pictures.

[0104] Table 7 Test Grouping

[0105]

[0106] Immunofluorescence test results Figure 1 , Figure 2 shown.

[0107] Compared with the BC group, the DSC1 content of the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the DSC1 content of the PC group increased significantly, with an increase rate of 155.17%, indicating that the positive control of this test was effective. Compared with the NC group, the DSC1 content of Examples 1-4 and Comparative Examples 1-6 increased significantly, with increase rates of 110.34%, 165.52%, 196.55%, 144.83%, 55.17%, 34.48%, 51.72%, 58.62%, 48.28%, and 55.17%, respectively. Examples 2 and 3 had the most significant promoting effects, and the increase rates of the examples were higher than those of the comparative examples, indicating that the synergistic effect of the seabuckthorn extract extracted by method 1 was more significant; hyaluronic acid disaccharide had a more significant synergistic effect than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da) and glycerol glucoside, and seabuckthorn extract; the soothing and repairing composition can synergistically promote the expression of DSC1.

[0108] Compared with the BC group, the DSG1 content of the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the DSG1 content of the PC group increased significantly, with an increase rate of 196.88%, indicating that the positive control of this test was effective. Compared with the NC group, the DSG1 content of Examples 1-4 and Comparative Examples 1-6 increased significantly, with increase rates of 187.50%, 278.13%, 318.75%, 228.13%, 112.50%, 68.75%, 90.63%, 128.13%, 96.88%, and 87.50%, respectively. Examples 2 and 3 had the most significant promoting effects, and the increase rates of the examples were higher than those of the comparative examples, indicating that the synergistic effect of the seabuckthorn extract extracted by method 1 was more significant; hyaluronic acid disaccharide had a more significant synergistic effect than dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da) and glycerol glucoside, and seabuckthorn extract; the soothing and repairing composition can synergistically promote the expression of DSG1.

[0109] Immunohistochemistry test results Figure 3 shown.

[0110] Compared with the BC group, the Ki67 content in the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the Ki67 content in the PC group increased significantly, with an increase rate of 451.04%, indicating that the positive control of this test was effective. Compared with the NC group, the Ki67 content of Examples 1-4 and Comparative Examples 1-6 groups increased significantly, with the increase rates being 227.59%, 312.45%, 388.59%, 288.38%, 122.41%, 59.96%, 93.98%, 140.87%, 95.23% and 98.13%, respectively. Examples 2 and 3 had the most significant promoting effects, and the increase rates of the examples were all higher than those of the comparative examples, indicating that the synergistic effect of the seabuckthorn extract extracted by method 1 was more significant; the synergistic effect of hyaluronic acid disaccharide was more significant than that of dimethylsilanol hyaluronate, hydrolyzed sodium hyaluronate (<1W Da) and glycerol glucoside and seabuckthorn extract; the soothing and repairing composition can synergistically promote the expression of Ki67.

[0111] Test Example 4: Test results of the experiment on soothing and repairing the human body by the cosmetics containing the skin care composition of the present invention The prepared essence water product of Example 5 was tested for human skin efficacy, and 30 volunteers aged 18-40 (skin condition: sensitive skin (lactic acid stinging positive)) were sought for product testing. If the a* value of the red zone analysis at any visit time point after the use of the sample is significantly reduced compared with that before use (P<0.05), the product is considered to have the effect of soothing the skin, which is used to evaluate its soothing effect on the human face. If the transepidermal water loss TEWL at any visit time point after the use of the sample is significantly reduced compared with that before use (P<0.05), the product is considered to have the effect of repairing the skin barrier function, which is used to evaluate its repair effect on the human face.

[0112] Test environment: temperature: 20.1℃~21.8℃, relative humidity: 44%~50%;

[0113] Testing time: before using the product, 2 weeks and 4 weeks after using the sample;

[0114] Testing equipment: VISIA skin tester (CanField, USA); skin water loss tester AquaFlux (AF200, BIOX, UK).

[0115] Facial red zone test: The VISIA skin detector was used to collect images of the left, middle and right sides of the subject's face under three light sources: standard light 1, standard light 2 and cross-polarized light, and the red zone images were derived under cross-polarized light. The red zone images were analyzed to obtain the quantitative index red zone analysis a* value, which was used to evaluate the improvement of facial skin redness and sensitivity.

[0116] Transepidermal water loss (TEWL) test: The skin water loss tester Aqua Flux is used. The instrument uses a single silicon crystal chip as a sensor for water vapor temperature and humidity to measure the flow density of water vapor and then determine the transepidermal water loss of the skin.

[0117] Calculation formula:

[0118] SPSS Statistics 25 was used for statistical analysis, with two-tailed test and test level α = 0.05.

[0119] The measurement data were analyzed by the normal distribution test results and the difference analysis method was selected: if the measured values ​​were normally distributed, the t-test method was used for statistical analysis; if they were non-normally distributed, the rank sum test method was used for statistical analysis.

[0120] The results of the facial red zone test are shown in Tables 8 and 9:

[0121] like Figure 4The following are pictures showing the changes in the red area on the faces of two subjects before and after using the samples.

[0122] Table 8 Descriptive statistics of a* value of red zone analysis (n=30)

[0123]

[0124] Table 9 Statistical analysis results of a* value in red zone analysis (n=30)

[0125]

[0126] Statistical analysis results: “-”: no statistically significant difference (P≥0.05); “*”: statistically significant difference (0.01≤P<0.05); “**”: statistically significant difference (0.001≤P<0.01); “***”: statistically significant difference (P<0.001).

[0127] The red zone analysis a* value test results showed that compared with before use, after 2 weeks of using the test sample, the red zone analysis a* value was significantly reduced (0.001≤P<0.01), and after 4 weeks of using the test sample, the red zone analysis a* value was significantly reduced (P<0.001), indicating that products containing the soothing and repairing skin care composition have soothing effects.

[0128] The results of the transepidermal water loss TEWL test are shown in Tables 10 and 11:

[0129] Table 10 Descriptive statistics of transepidermal water loss TEWL values ​​(n=30)

[0130]

[0131] Table 11 Statistical analysis results of transepidermal water loss TEWL values ​​(n=30)

[0132]

[0133] (Note: Statistical analysis results: “-”: no statistically significant difference (P≥0.05); “*”: statistically significant difference (0.01≤P<0.05); “**”: statistically significant difference (0.001≤P<0.01); “***”: statistically significant difference (P<0.001).)

[0134] The test results showed that after using cosmetics containing 3.0% soothing and repairing composition for 2 weeks and 4 weeks, the transepidermal water loss TEWL of the skin was significantly reduced, which was reduced by 17.87% and 25.29% respectively compared with before use, indicating that cosmetics containing soothing and repairing skin care composition have a repairing effect.

[0135] It should be noted that the above examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art may modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A composition with soothing and repairing effects, characterized in that: The composition comprises the following components in parts by weight: 0.01-1 part of glyceryl glucoside, 0.005-0.2 part of hyaluronic acid disaccharide, 1-12 parts of sea buckthorn extract.

2. The composition according to claim 1, characterized in that The seabuckthorn extract is obtained by any of the following extraction and preparation methods; specifically, Extraction method 1 is as follows: S1-1, mixing the crushed seabuckthorn fruit with water and subjecting the mixture to microwave extraction, subjecting the obtained mixture to vacuum decompression reflux extraction, and filtering the extract to obtain a filtrate; S1-2, the filtrate obtained in step S1 is concentrated under reduced pressure, ethanol is added, and the mixture is allowed to stand at low temperature. After filtering, the filtrate is concentrated under reduced pressure for a second time, 1,3-butanediol is added, and the mixture is stirred and mixed evenly. After filtering, the seabuckthorn extract is obtained; Extraction method 2 is as follows: S2-1, taking the crushed seabuckthorn fruit, adding ethanol aqueous solution to perform reflux extraction, filtering the filtrate, repeatedly extracting the residue 1-3 times, and combining the filtrate; S2-2, the filtrate obtained in step S1 is concentrated under reduced pressure, 1,3-butanediol is added thereto, the mixture is stirred and mixed evenly, and the sea buckthorn extract is obtained after filtering.

3. The composition according to claim 2, characterized in that In the step S1-1, the mass ratio of the crushed seabuckthorn fruit to water is 1:15-1:20; the microwave power is 300-400W, and the microwave treatment time is 20-30min; the extraction temperature of the vacuum decompression reflux extraction is 45-60°C, the extraction time is 0.5-2h, and the number of reflux extractions can be 1-3 times. When the number of reflux extractions is greater than 1 time, the extracts obtained each time are combined and filtered.

4. The composition according to claim 2, characterized in that In the step S1-2, the reduced pressure concentration temperature is controlled at 50-70°C, and the reduced pressure concentration is performed to 6-10 times the mass of the crushed sea buckthorn fruit in step S1; ethanol is added until the alcohol content in the obtained concentrated solution is 60-80%; the solution is placed at 4±2°C and allowed to stand for 10-30 hours; the reduced pressure concentration is performed again to 2-6 times the mass of the crushed sea buckthorn fruit in step S1, and then 1-1.5 times the mass of 1,3-butanediol is added to the obtained secondary concentrated solution.

5. The composition according to claim 2, characterized in that In the step S2-1, the ethanol concentration in the ethanol aqueous solution is 20-40%, the mass ratio of the crushed seabuckthorn fruit to the ethanol aqueous solution is 1:8-1:12, and the specific conditions of the reflux extraction are: reflux extraction at 60-80°C for 0.2-1h.

6. The composition according to claim 2, characterized in that In the step S2-2, the reduced pressure concentration temperature is controlled at 50-70° C., and the reduced pressure concentration is performed to 2-6 times the mass of the crushed sea buckthorn fruit in step S1; then 1,3-butanediol is added in an amount of 1-1.5 times the mass of the concentrate.

7. Use of the composition according to any one of claims 1 to 6 in products for soothing, repairing and skin care.

8. The use according to claim 7, characterized in that The product is a cosmetic, and further a skin care cosmetic.

9. A soothing and repairing skin care cosmetic, characterized in that: The cosmetic comprises at least the composition according to any one of claims 1 to 6; the cosmetic is a skin care cosmetic; Furthermore, based on the total mass of the soothing and repairing skin care cosmetic, the amount of the composition is 0.5-10.0%; Furthermore, based on the total mass of the soothing and repairing skin care cosmetic, the amount of the composition added is 2.0-5.0%.

10. The method for preparing the soothing and repairing skin care cosmetics according to claim 9, characterized in that: The preparation method comprises the step of mixing the composition according to any one of claims 1 to 6 and other raw material components.

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