High-precision acquisition electrophoresis system based on microfluidics

Abstract

The invention discloses a high-precision acquisition electrophoresis system based on microfluidics, and the high-precision acquisition electrophoresis system based on microfluidics comprises a high-precision acquisition electrophoresis system based on micro-fluidic chip driving, the high-precision acquisition electrophoresis system based on micro-fluidic chip driving comprises a medical sample processing module, a micro-fluidic chip module, a high-voltage driving module, a high-precision detection module, a data analysis module and a control module, the control module is electrically connected with the medical sample processing module, and the high-precision acquisition electrophoresis system relates to the technical field of high-precision acquisition electrophoresis. The medical sample treatment module is arranged to pretreat the medical sample, purify the medical sample and remove impurities in the medical sample, so that the purity of the medical sample is improved, and the accuracy of a subsequent detection result is also improved; the components of the medical sample are converted into corresponding voltage and current signals through the high-precision detection module, and the voltage and current signals are analyzed through the data analysis module, so that the detection result is more accurate.

Description

Technical Field

[0001] The invention relates to the technical field of high-precision collection electrophoresis, in particular to a high-precision collection electrophoresis system based on microfluidics. Background Art

[0002] With the development of science and technology, high-precision collection electrophoresis is combined with microfluidic chip technology to integrate multiple high-precision collection electrophoresis separation channels and related medical sample processing and detection functions on a single chip. The integrated high-precision collection electrophoresis system greatly simplifies the analysis process, reduces sample transfer and operation steps, and improves analysis efficiency and accuracy. Secondly, through the use of microfluidic chips, the consumption of samples and reagents is greatly reduced, and the cost is also reduced accordingly. However, the traditional high-precision acquisition electrophoresis system driven by microfluidic chips still has many shortcomings: the medical sample processing module in the traditional system processes the medical samples in multiple steps, including pretreatment, separation and purification, which increases the complexity and difficulty of operation of the system; the microfluidic chips in the traditional system are usually designed and manufactured for specific analysis targets, and new chips are redesigned and manufactured for different analysis requirements, which limits the flexibility and scalability of the system; the detection module may have the problem of low detection sensitivity in some cases, which may not be sensitive enough for the analysis and detection of low-concentration samples; although it is an integrated system, the various modules in the traditional system are still independently operated and controlled, and the degree of integration is limited. Summary of the invention

[0003] The purpose of the present invention is to provide a high-precision collection electrophoresis system based on microfluidics to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solutions: A high-precision collection electrophoresis system based on microfluidics, the high-precision collection electrophoresis system based on microfluidics includes a high-precision collection electrophoresis system based on microfluidic chip drive, the high-precision collection electrophoresis system based on microfluidic chip drive includes a medical sample processing module, a microfluidic chip module, a high-voltage drive module, a high-precision detection module, a data analysis module and a control module, the control module is electrically connected to the medical sample processing module, the control module is electrically connected to the microfluidic chip module, the control module is electrically connected to the high-precision detection module, and the control module is electrically connected to the data analysis module; The medical sample processing module is responsible for pre-processing the medical samples according to medical standards and introducing the pre-processed medical samples into the sampling channel of the microfluidic chip module through the sampling channel of the medical sample processing module, and mixing the medical samples with other processing liquids in the sampling channel of the microfluidic chip module; The medical sample processing module and the microfluidic chip module are connected via a channel; The microfluidic chip module is provided with a separation channel, a sample outlet channel and a sample inlet channel inside, and the microfluidic chip module provides an appropriate electric field strength to drive the medical sample to separate in the separation channel; The high-voltage driving module is responsible for providing a stable high-voltage electric field and is electrically connected to the separation channel of the microfluidic chip module through electrodes; The control parameters and voltage output of the high-voltage drive module match the separation requirements of the microfluidic chip module; After the microfluidic chip module completes the separation of the medical sample, the separated components are introduced into the sample inlet channel of the high-precision detection module through the sample outlet channel of the microfluidic chip module; the high-precision detection module detects and analyzes the separated components; The high-precision detection module is internally integrated with a high-precision current and voltage acquisition and data conversion circuit, which converts the separated components into corresponding current and voltage signals through the high-precision current and voltage acquisition and data conversion circuit; The data analysis module is electrically connected to the high-precision detection module to analyze the current and voltage signals and provide real-time analysis results; The control module regulates and controls the entire system, performs data exchange and instruction transmission among various modules, and regulates and controls medical sample processing, separation, detection and system parameters.

[0005] In a preferred embodiment of the present invention, the medical sample processing module is mainly used for preliminary processing of medical samples, including pretreatment of medical samples, purification and separation of medical samples; The medical standards for pretreatment of medical samples are GB19489, WS / T402, and WS / T403; The microfluidic chip module is the core component of the high-precision collection electrophoresis system based on microfluidics, which is used for the detection and separation of medical samples. It consists of separation channels, electrodes and detection channels, and performs efficient separation and sensitive detection of medical samples. In the microfluidic chip module, the separation channel structure and electrode layout are designed to perform the separation and detection functions of medical samples; The microfluidic chip module selects new materials, including polymers, ceramics and nanomaterials, and applies advanced preparation technologies, including 3D printing, nanofabrication and self-assembly; Microfluidic chip modules are designed with separation channel structures with special shapes and functions, including microarrays, micromixers, microreactors, and nanopore arrays; The microfluidic chip module integrates multiple analysis technologies on the microfluidic chip, including spectral analysis, electrochemical detection, mass spectrometry and biosensors. By introducing intelligent control systems and automated operation technologies, the microfluidic chip module can be automatically controlled and remotely monitored.

[0006] In a preferred embodiment of the present invention, designing the separation channel structure and electrode layout specifically includes: According to the separation target and separation efficiency requirements, select the channel shape, including linear, spiral, and Y-shaped, and determine the channel width, depth, and length dimension parameters; introduction of separation-enhancing structures, including micropillar arrays, microwell arrays, and micromixers; Separation channel structure design includes: Design the inlet and outlet to ensure that the sample can smoothly enter and exit the separation channel, using micro pump and micro valve devices; According to the connection mode and integration capability of the separation channel, the separation channel is connected and integrated with the sample processing module, the high-voltage driving module and the detection module; Electrode layout design includes: Determine the position and layout of the electrodes to ensure that a uniform electric field can be applied in the separation channel. The layout of the electrodes includes electrodes at both ends, electrodes on both sides, and electrodes at four corners. Select the shape and size of the electrode to ensure the uniformity and stability of the electric field. The electrode shapes include straight line, arrow, and circle. The size selection takes into account the channel width and electric field strength factors. Design isolation and shielding structures for electrodes; Corresponding detection electrodes are set at the locations where electrochemical and optical detection is performed to monitor and record sample signals during the separation process in real time.

[0007] In a preferred embodiment of the present invention, a high-voltage driving module is used to provide a high-voltage electric field to drive the sample to perform electrophoretic separation in a microfluidic chip, and includes a high-voltage power supply, a voltage controller and an electrode interface to ensure that a stable high-voltage electric field is applied to the microfluidic chip. The high-voltage setting range of the high-voltage driving module is 1000-3000V.

[0008] In a preferred embodiment of the present invention, the high-precision detection module is used to detect and analyze medical samples, and convert the components inside the medical samples into corresponding current and voltage signals.

[0009] In a preferred embodiment of the present invention, the control module is electrically connected to the medical sample processing module, the control module is electrically connected to the microfluidic chip module, the control module is electrically connected to the high-precision detection module, and the control module is electrically connected to the data analysis module.

[0010] A high-precision collection electrophoresis system based on microfluidics includes the following specific operation steps: Step 1: The medical sample processing module pre-processes the medical sample according to medical standards; Step 2: The microfluidic chip module and the high-voltage driving module cooperate with each other to separate the medical samples; Step 3: The high-precision detection module analyzes and detects the medical sample and converts it into corresponding current and voltage signals; Step 4: The data analysis module analyzes the current and voltage signals to determine the composition and concentration of the medical sample.

[0011] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention.

[0012] The present invention pre-treats the medical samples by setting up a medical sample processing module, purifies the medical samples, removes impurities in the medical samples, improves the purity of the medical samples, and improves the accuracy of subsequent test results. In addition, the present invention converts the components of the medical samples into corresponding voltage and current signals through a high-precision detection module, and the data analysis module analyzes the voltage and current signals to make the test results more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which: Figure 1 It is a structural schematic diagram of the overall system in the present invention; Figure 2 It is a schematic diagram of the system structure of the separation channel in the present invention; Figure 3 It is a structural schematic diagram of the electrode layout in the present invention; Figure 4 The circuit diagram of the current acquisition part in the high-precision detection module of the present invention; Figure 5 This is a circuit diagram of the voltage acquisition part in the high-precision detection module of the present invention. DETAILED DESCRIPTION

[0014] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0015] Reference Figure 1As shown, the high-precision collection electrophoresis system based on microfluidics includes a high-precision collection electrophoresis system driven by a microfluidic chip, and the high-precision collection electrophoresis system driven by a microfluidic chip includes a medical sample processing module, a microfluidic chip module, a high-voltage drive module, a high-precision detection module, a data analysis module and a control module, the control module is electrically connected to the medical sample processing module, the control module is electrically connected to the microfluidic chip module, the control module is electrically connected to the high-precision detection module, and the control module is electrically connected to the data analysis module; The medical sample processing module is mainly used for the preliminary processing of medical samples, including the pretreatment, purification and separation of medical samples; The medical sample processing module is responsible for pre-processing the medical samples according to medical standards and introducing the pre-processed medical samples into the sampling channel of the microfluidic chip module through the sampling channel of the medical sample processing module, and mixing the medical samples with other processing liquids in the sampling channel of the microfluidic chip module; The medical sample processing module and the microfluidic chip module are connected via a channel; The microfluidic chip module is provided with a separation channel, a sample outlet channel and a sample inlet channel inside, and the microfluidic chip module provides an appropriate electric field strength to drive the medical sample to separate in the separation channel; The high-voltage driving module is responsible for providing a stable high-voltage electric field and is electrically connected to the separation channel of the microfluidic chip module through electrodes; The control parameters and voltage output of the high-voltage drive module match the separation requirements of the microfluidic chip module; After the microfluidic chip module completes the separation of the medical sample, the separated components are introduced into the sample inlet channel of the high-precision detection module through the sample outlet channel of the microfluidic chip module; the high-precision detection module detects and analyzes the separated components; The high-precision detection module is internally integrated with a high-precision current and voltage acquisition and data conversion circuit, which converts the separated components into corresponding current and voltage signals through the high-precision current and voltage acquisition and data conversion circuit; The data analysis module is electrically connected to the high-precision detection module to analyze the current and voltage signals and provide real-time analysis results; The control module regulates and controls the entire system, performs data exchange and instruction transmission among various modules, and regulates and controls medical sample processing, separation, detection and system parameters.

[0016] The specific usage scenario of this embodiment is: Specifically, the sample pretreatment is specifically implemented as follows: Collect medical samples that need to be analyzed according to medical standards GB19489, WS / T402, and WS / T403, and ensure that the sampling method meets the analysis requirements to avoid sample contamination or loss; For liquid samples, proper mixing should be performed to ensure sample uniformity and avoid local component deviation; For samples with a lot of suspended matter, such as suspensions or biological samples, use filter paper, filter membranes, or centrifugation to remove solid particles or macromolecules; If necessary, dilute the sample appropriately to make the sample concentration fit the working range of the analytical method and avoid interference caused by excessive concentration; Adjust the acidity and alkalinity of the sample to meet the requirements of the analytical method. Acid or alkaline solutions can be used for pH adjustment; For complex samples or samples that need to be enriched with target substances, extraction or extraction methods, such as liquid-liquid extraction, solid phase extraction, solid phase microextraction, etc., can be used to enhance the analytical signal; For complex or interfering substances in some samples, chemical methods or enzyme treatment can be used to degrade or convert them into analyzable forms; For surface attachments or residues, a sample washing step can be performed to remove the interferences; Sample preservation: According to the requirements of the analytical method, the processed samples should be properly preserved to maintain the stability and reliability of the samples; The medical sample processing module and the microfluidic chip module are connected through a channel, and the medical sample processing module and the microfluidic chip module cooperate with each other; Reference Figure 2 As shown, the microfluidic chip module is the core component of the high-precision collection electrophoresis system based on microfluidics, which is used for the detection and separation of medical samples. It consists of a separation channel, an electrode and a detection channel, and performs efficient separation and sensitive detection of medical samples. In the microfluidic chip module, the separation channel structure and electrode layout are designed to perform the separation and detection functions of medical samples; The microfluidic chip module is provided with a separation channel, a sample outlet channel and a sample inlet channel inside, and the microfluidic chip module provides an appropriate electric field strength to drive the medical sample to separate in the separation channel; The microfluidic chip module is the core component of the high-precision collection electrophoresis system based on microfluidics, which is used for the detection and separation of medical samples; Microfluidic chips are usually composed of separation channels, electrodes and detection channels, which can perform efficient separation and sensitive detection of medical samples. In the microfluidic chip module, the separation channel structure and electrode layout are designed to perform the separation and detection functions of medical samples. The microfluidic chip module selects new materials, including polymers, ceramics and nanomaterials, and applies advanced preparation technologies, including 3D printing, nanofabrication and self-assembly; Microfluidic chip modules are designed with separation channel structures with special shapes and functions, including microarrays, micromixers, microreactors, and nanopore arrays; The microfluidic chip module integrates a variety of analytical technologies on the microfluidic chip, including spectral analysis, electrochemical detection, mass spectrometry and biosensors. By introducing intelligent control systems and automated operation technologies, the microfluidic chip module can be automatically controlled and remotely monitored. The specific usage scenario of this embodiment is: Specifically, the microfluidic chip module is designed to be reconfigurable, so that it can flexibly adapt to different analysis needs, and through modular design and exchangeable components, different functional modules can be quickly replaced and combined.

[0017] Reference Figure 3 As shown, the design of the separation channel structure and electrode layout specifically includes the following steps: The steps for designing the separation channel structure are as follows: According to the separation target and separation efficiency requirements, select the channel shape, including linear, spiral, and Y-shaped, and determine the channel width, depth, and length dimension parameters; introduction of separation-enhancing structures, including micropillar arrays, microwell arrays, and micromixers; Design the inlet and outlet to ensure that the sample can smoothly enter and exit the separation channel, using micro pump and micro valve devices; According to the connection mode and integration capability of the separation channel, the separation channel is connected and integrated with the sample processing module, the high-voltage driving module and the detection module; The steps for electrode layout design are as follows: Determine the position and layout of the electrodes to ensure that a uniform electric field can be applied in the separation channel. The layout of the electrodes includes electrodes at both ends, electrodes on both sides, and electrodes at four corners. Select the shape and size of the electrode to ensure the uniformity and stability of the electric field. The electrode shapes include straight line, arrow, and circle. The size selection takes into account the channel width and electric field strength factors. Design isolation and shielding structures for electrodes; Corresponding detection electrodes are set at the locations where electrochemical and optical detection is performed to monitor and record sample signals during the separation process in real time.

[0018] The high-voltage driving module is responsible for providing a stable high-voltage electric field and is electrically connected to the separation channel of the microfluidic chip module through electrodes; The control parameters and voltage output of the high-voltage drive module match the separation requirements of the microfluidic chip module; The high-voltage drive module is used to provide a high-voltage electric field to drive the sample to perform electrophoretic separation in the microfluidic chip. It consists of a high-voltage power supply, a voltage controller, and an electrode interface to ensure that a stable high-voltage electric field is applied to the microfluidic chip. The high-voltage setting range of the high-voltage drive module is 1000-3000V. The specific usage scenario of this embodiment is: when the medical sample molecules enter the separation channel, according to the characteristics of the medical sample, the control module sets the parameters matching the characteristics of the medical sample and the voltage provided by the required high-voltage driving module, so as to achieve the separation of the medical sample; After the microfluidic chip module completes the separation of the medical sample, the separated components are introduced into the sampling channel of the high-precision detection module through the sampling channel of the microfluidic chip module; the high-precision detection module detects and analyzes the separated components.

[0019] Reference Figure 4-5 As shown, the high-precision detection module is used to detect and analyze medical samples, and convert the components inside the medical samples into corresponding current and voltage signals; The specific usage scenario of this embodiment is as follows: a medical sample holding cavity is provided inside the high-precision detection module, and the medical sample holding cavity is heated to convert the medical sample into gas molecules. Due to the different concentrations and components of the gas molecules, the resistivity in the high-precision current and voltage acquisition and data conversion circuit will change. The resistivity is converted into corresponding current and voltage signals. The data analysis module analyzes and detects according to the current and voltage signals to determine the composition and concentration of the medical sample.

[0020] The present invention specifically comprises the following steps when used: Step 1: The medical sample processing module pre-processes the medical sample according to medical standards; Step 2: The microfluidic chip module and the high-voltage driving module cooperate with each other to separate the medical samples; Step 3: The high-precision detection module analyzes and detects the medical sample and converts it into corresponding current and voltage signals; Step 4: The data analysis module analyzes the current and voltage signals to determine the composition and concentration of the medical sample.

[0021] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

[0022] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A high-precision collection electrophoresis system based on microfluidics, the high-precision collection electrophoresis system based on microfluidics includes a high-precision collection electrophoresis system driven by a microfluidic chip, characterized in that: The high-precision collection electrophoresis system based on microfluidic chip drive includes a medical sample processing module, a microfluidic chip module, a high-voltage drive module, a high-precision detection module, a data analysis module and a control module; The medical sample processing module is responsible for pre-processing the medical samples according to medical standards and introducing the pre-processed medical samples into the sampling channel of the microfluidic chip module through the sampling channel of the medical sample processing module, and mixing the medical samples with other processing liquids in the sampling channel of the microfluidic chip module; The medical sample processing module and the microfluidic chip module are connected via a channel; The microfluidic chip module is provided with a separation channel, a sample outlet channel and a sample inlet channel inside, and the microfluidic chip module provides an appropriate electric field strength to drive the medical sample to separate in the separation channel; The high-voltage driving module is responsible for providing a stable high-voltage electric field and is electrically connected to the separation channel of the microfluidic chip module through electrodes; The control parameters and voltage output of the high-voltage drive module match the separation requirements of the microfluidic chip module; After the microfluidic chip module completes the separation of the medical sample, the separated components are introduced into the sample inlet channel of the high-precision detection module through the sample outlet channel of the microfluidic chip module; The high-precision detection module detects and analyzes the separated components; The high-precision detection module is internally integrated with a high-precision current and voltage acquisition and data conversion circuit, which converts the separated components into corresponding current and voltage signals through the high-precision current and voltage acquisition and data conversion circuit; The data analysis module is electrically connected to the high-precision detection module to analyze the current and voltage signals and provide real-time analysis results; The control module regulates and controls the entire system, performs data exchange and instruction transmission among various modules, and regulates and controls medical sample processing, separation, detection and system parameters.

2. A high-precision collection electrophoresis system based on microfluidics according to claim 1, characterized in that: The medical sample processing module is mainly used for the preliminary processing of medical samples, including the pretreatment, purification and separation of medical samples; The medical standards for pretreatment of medical samples are GB19489, WS / T402, and WS / T403; The microfluidic chip module is the core component of the high-precision collection electrophoresis system based on microfluidics, which is used for the detection and separation of medical samples. It consists of separation channels, electrodes and detection channels, and performs efficient separation and sensitive detection of medical samples. In the microfluidic chip module, the separation channel structure and electrode layout are designed to perform the separation and detection functions of medical samples; The microfluidic chip module selects new materials, including polymers, ceramics and nanomaterials, and applies advanced preparation technologies, including 3D printing, nanofabrication and self-assembly; Microfluidic chip modules are designed with separation channel structures with special shapes and functions, including microarrays, micromixers, microreactors, and nanopore arrays; The microfluidic chip module integrates multiple analysis technologies on the microfluidic chip, including spectral analysis, electrochemical detection, mass spectrometry and biosensors. By introducing intelligent control systems and automated operation technologies, the microfluidic chip module can be automatically controlled and remotely monitored.

3. The high-precision collection electrophoresis system based on microfluidics according to claim 2, characterized in that: The details of designing separation channel structure and electrode layout include: According to the separation target and separation efficiency requirements, select the channel shape, including linear, spiral, and Y-shaped, and determine the channel width, depth, and length dimension parameters; introduction of separation-enhancing structures, including micropillar arrays, microwell arrays, and micromixers; Separation channel structure design includes: Design the inlet and outlet to ensure that the sample can smoothly enter and exit the separation channel, using micro pump and micro valve devices; According to the connection mode and integration capability of the separation channel, the separation channel is connected and integrated with the sample processing module, the high-voltage driving module and the detection module; Electrode layout design includes: Determine the position and layout of the electrodes to ensure that a uniform electric field can be applied in the separation channel. The layout of the electrodes includes electrodes at both ends, electrodes on both sides, and electrodes at four corners. Select the shape and size of the electrode to ensure the uniformity and stability of the electric field. The electrode shapes include straight line, arrow, and circle. The size selection takes into account the channel width and electric field strength factors. Design isolation and shielding structures for electrodes; Corresponding detection electrodes are set at the locations where electrochemical and optical detection is performed to monitor and record sample signals during the separation process in real time.

4. The high-precision collection electrophoresis system based on microfluidics according to claim 1, characterized in that: The high-voltage drive module is used to provide a high-voltage electric field to drive the sample to perform electrophoretic separation in the microfluidic chip. It includes a high-voltage power supply, a voltage controller and an electrode interface to ensure that a stable high-voltage electric field is applied to the microfluidic chip. The high-voltage setting range of the high-voltage drive module is 1000-3000V.

5. The high-precision collection electrophoresis system based on microfluidics according to claim 1, characterized in that: The high-precision detection module is used to detect and analyze medical samples, converting the components inside the medical samples into corresponding current and voltage signals.

6. The high-precision collection electrophoresis system based on microfluidics according to claim 1, characterized in that: The control module is electrically connected to the medical sample processing module, the control module is electrically connected to the microfluidic chip module, the control module is electrically connected to the high-precision detection module, and the control module is electrically connected to the data analysis module.

7. A high-precision collection electrophoresis system based on microfluidics according to any one of claims 1 to 6, characterized in that: The specific steps are as follows: Step 1: The medical sample processing module pre-processes the medical sample according to medical standards; Step 2: The microfluidic chip module and the high-voltage driving module cooperate with each other to separate the medical samples; Step 3: The high-precision detection module analyzes and detects the medical sample and converts it into corresponding current and voltage signals; Step 4: The data analysis module analyzes the current and voltage signals to determine the composition and concentration of the medical sample.

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