Membrane separation and cavity ring-down spectroscopy coupled lung cancer expiration trace detection system

Abstract

The invention provides a membrane separation and cavity ring-down spectroscopy coupled lung cancer expiration trace detection system, and belongs to the technical field of medical treatment. Comprising the following steps: preparing a gas separation membrane; s2, coupling the gas separation membrane with the CRDS system; s3, developing a lung cancer screening expiration analysis system based on separation-sensing; a green and high-efficiency membrane separation method is used for solving the problem of spectrum interference which still cannot be avoided based on laser selectivity in exhaled gas detection, a POCT gas sensing system based on separation-sensing high-selectivity and high-sensitivity lung cancer screening exhaled gas analysis is developed, and a new technology is provided for lung cancer exhaled gas screening.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to a lung cancer breath trace detection system coupled with membrane separation and optical cavity ring-down spectroscopy. Background Art

[0002] Lung cancer, also known as primary bronchogenic carcinoma, is the most common lung malignancy originating from the trachea, bronchial mucosa or glands. The clinical symptoms of lung cancer are closely related to the size, type, development stage, location, complications and metastasis of the tumor. Common symptoms include cough, blood in sputum, wheezing, chest pain, etc. There may be no obvious symptoms in the early stages of the disease, and some patients are accidentally discovered during physical examinations.

[0003] CRDS (Cavity Ring-Down Spectroscopy) is a very sensitive spectroscopic method. It can be used to detect the absolute optical extinction of a sample, including light scattering and absorption. It has been widely used to detect the absorption of gaseous samples at specific wavelengths and can determine the mole fraction of a sample at the parts per trillion level.

[0004] When treating lung cancer patients in medical treatment, in order to more accurately determine the treatment plan, it is usually necessary to test the patient's lung cancer markers. Traditional detection methods cannot solve the problem of laser-based selectivity in the patient's exhaled gas detection and cannot avoid spectral interference, which in turn affects the accuracy of the detection, affects the subsequent customization of treatment plans, and further affects the patient's treatment and recovery.

[0005] Therefore, the present application provides a lung cancer breath trace detection system coupling membrane separation and optical cavity ring-down spectroscopy to meet the needs. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a lung cancer breath trace detection system coupling membrane separation and optical cavity ring-down spectroscopy to solve the problem of existing spectral interference.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions: a lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy, comprising the following steps: S1: Preparation of gas separation membranes; a. Identify the molecular substance with the highest frequency among lung cancer markers and define it as the target gas; b. Perform spectral interference analysis on the molecular substance with the highest frequency, analyze the targeted gas, and identify it as the interfering gas; c. According to the characteristics of the target gas and interfering gas, prepare a hollow fiber membrane and a modified functional layer that can effectively separate gases and avoid the influence of the membrane material itself on spectral detection; S2: coupling of gas separation membrane and CRDS system; a. Test the performance of the CRDS system from multiple perspectives; b. Use standard gas as sample to compare with the gold standard GC-MS for trace gas detection to verify the accuracy of the system; c. Use the gas separation membrane and CRDS system joint detection system to measure the changes in gas concentration of single standard gas, mixed gas and human exhaled gas before and after adding the gas separation membrane, verify the coupling and adaptation degree of the gas separation membrane and the CRDS system, and selectively and accurately separate the interfering gas at the ultra-trace level (ppbv) without introducing other gas interferences; S3: Development of a breath analysis system for lung cancer screening based on separation-sensing; a. Quasi-single-mode high pulse frequency laser light source; b, two sample chambers treated with silanization and inertness; c. High reflectivity mirror; d. Photoelectric detection module; e. Data processing module; f. Online measurement and sampling equipment based on gas separation membrane.

[0008] Preferably, in step 1, there are two target gases, namely styrene (C8H8) and ethylbenzene (C8H10), and the interfering gas is acetone.

[0009] Preferably, the properties according to which the hollow fiber membrane and the modified functional layer are prepared in step 1 are: polarity, hydrophilicity and hydrophobicity and analytical size.

[0010] Preferably, in step 1, the thickness and density of the modified functional layer are optimized by studying the membrane structure and performance to achieve accurate separation of the target gas and the interfering gas; the gas separation performance of the modified membrane is evaluated using a PTR-MS system, and the preparation process of the separation membrane is further optimized based on the detection characteristics of CRDS to prepare a separation membrane for separating ultra-trace (ppbv) level interfering gases.

[0011] Preferably, the directions for measuring the performance of the CRDS system in step 2 are divided into: the stability of the operation of the CRDS system, the detection limit of the CRDS system, the linearity of the CRDS system, the repeatability of the CRDS system, the response speed of the CRDS system and the accuracy of the CRDS system. Testing from multiple angles can confirm the overall effect of the CRDS system.

[0012] Preferably, the single standard gas detected in step 2 is: target gas and interfering gas; the mixed gas is: a mixture of target gas and interfering gas.

[0013] Preferably, in step 2, exhaled breath is used as a sample and compared with the gold standard GC-MS for trace gas detection to verify the accuracy of the combined system.

[0014] Preferably, the frequency of the quasi-single-mode high-pulse-frequency laser light source in step 3 needs to be greater than one kilohertz, that is, (>1 kHz); the reflectivity of the high-reflectivity reflector is >99.985%.

[0015] Compared with the prior art, the present invention has at least the following beneficial effects: In the above scheme, a green and efficient membrane separation method is used to solve the spectral interference problem that cannot be avoided in exhaled gas detection based on laser selectivity, and a POCT gas sensing system for exhaled breath analysis for lung cancer screening with high selectivity and sensitivity based on separation-sensing is developed, providing new technology for exhaled breath screening for lung cancer. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable those skilled in the relevant art to make and use the present disclosure.

[0017] Figure 1 The following is the overall flow chart; Figure 2 Flow chart for step 1; Figure 3 Flow chart for step 2; Figure 4 Flowchart for step 3.

[0018] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments, and the adjustments or modifications made are still included in the scope of the attached claims. DETAILED DESCRIPTION

[0019] In the following, a lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy provided by the present invention is described in detail in conjunction with the accompanying drawings and specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternative methods to implement some known technologies; and the accompanying drawings are only for a more specific description of the embodiments, and are not intended to specifically limit the present invention.

[0020] It should be noted that the references to "one embodiment", "embodiment", "exemplary embodiments", "some embodiments" and the like in the specification indicate that the embodiments described may include specific features, structures or characteristics, but not every embodiment may include the specific features, structures or characteristics. In addition, when a specific feature, structure or characteristic is described in conjunction with an embodiment, it should be within the knowledge of a person skilled in the art to implement such feature, structure or characteristic in conjunction with other embodiments (whether or not explicitly described).

[0021] In general, a term can be understood, at least in part, from its use in context. For example, depending, at least in part, on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending, at least in part, on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0022] It will be understood that the meaning of “on,” “over,” and “above” in this disclosure should be interpreted in the broadest manner, so that “on” means not only “directly on” something, but also includes the meaning of being “on” something with intervening features or layers therebetween, and “on” or “over” means not only “on” or “above” something, but also includes the meaning of being “on” or “above” something with no intervening features or layers therebetween.

[0023] Additionally, spatially relative terms such as "under," "beneath," "lower," "above," "upper," and the like may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as shown in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein may be similarly interpreted accordingly.

[0024] In this embodiment, if Figures 1 to 4 As shown, an embodiment of the present invention provides a lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy, comprising the following steps: S1: Preparation of gas separation membranes; a. Identify the molecular substance with the highest frequency among lung cancer markers and define it as the target gas; b. Perform spectral interference analysis on the molecular substance with the highest frequency, analyze the targeted gas, and identify it as the interfering gas; In the above scheme, there are two target gases, namely: styrene (C8H8) and ethylbenzene (C8H10), and the interfering gas is: acetone; c. According to the characteristics of the target gas and interfering gas, prepare a hollow fiber membrane and a modified functional layer that can effectively separate gases and avoid the influence of the membrane material itself on spectral detection; The properties according to which the hollow fiber membrane and the modified functional layer are prepared in the above scheme are: polarity, hydrophilicity and hydrophobicity, and analytical size; In the above scheme, the thickness and density of the modified functional layer are optimized through the study of membrane structure and performance, so as to achieve accurate separation of target gas and interfering gas; the gas separation performance of the modified membrane is evaluated by using the PTR-MS system, and the preparation process of the separation membrane is further optimized according to the detection characteristics of CRDS, so as to prepare a separation membrane for separating ultra-trace (ppbv) level interfering gases; S2: coupling of gas separation membrane and CRDS system; a. Test the performance of the CRDS system from multiple perspectives; The directions for measuring the performance of the CRDS system in the above scheme are divided into: the stability of the CRDS system operation, the detection limit of the CRDS system, the linearity of the CRDS system, the repeatability of the CRDS system, the response speed of the CRDS system and the accuracy of the CRDS system. Testing from multiple angles can confirm the overall effect of the CRDS system; By measuring the CRDS system from multiple angles, the stability, tolerance, linearity, repeatability, response speed and accuracy of the CRDS system are ensured.

[0025] b. Use standard gas as sample to compare with the gold standard GC-MS for trace gas detection to verify the accuracy of the system; The single standard gas detected in the above scheme is: target gas and interference gas; the mixed gas is: a mixture of target gas and interference gas; c. Use the gas separation membrane and CRDS system joint detection system to measure the changes in gas concentration of single standard gas, mixed gas and human exhaled gas before and after adding the gas separation membrane, verify the coupling and adaptation degree of the gas separation membrane and the CRDS system, and selectively and accurately separate the interfering gas at the ultra-trace level (ppbv) without introducing other gas interferences; In the above scheme, exhaled breath was used as a sample and compared with the gold standard GC-MS for trace gas detection to verify the accuracy of the combined system; S3: Development of a breath analysis system for lung cancer screening based on separation-sensing; a. Quasi-single-mode high pulse frequency laser light source; b, two sample chambers treated with silanization and inertness; c. High reflectivity mirror; d. Photoelectric detection module; e. Data processing module; f. Online measurement and sampling equipment based on gas separation membrane; In the above scheme, the frequency of the quasi-single-mode high-pulse frequency laser light source needs to be greater than one kilohertz, that is (>1KHZ); the reflectivity of the high-reflectivity mirror is >99.985%. By limiting the frequency and reflectivity, it is ensured that the system can operate in a better state and achieve more accurate detection.

[0026] The present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention. In order to make the public have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention, but those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0027] A person skilled in the art will appreciate that all or part of the steps in the above-mentioned embodiment method can be completed by instructing related hardware through a program, and the program can be stored in a computer-readable storage medium, such as ROM / RAM, a disk, an optical disk, etc.

[0028] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy, characterized in that: The following steps are involved: S1: Preparation of gas separation membranes; a. Identify the molecular substance with the highest frequency among lung cancer markers and define it as the target gas; b. Perform spectral interference analysis on the molecular substance with the highest frequency, analyze the targeted gas, and identify it as the interfering gas; c. According to the characteristics of the target gas and interfering gas, prepare a hollow fiber membrane and a modified functional layer that can effectively separate gases and avoid the influence of the membrane material itself on spectral detection; S2: coupling of gas separation membrane and CRDS system; a. Test the performance of the CRDS system from multiple perspectives; b. Use standard gas as sample to compare with the gold standard GC-MS for trace gas detection to verify the accuracy of the system; c. Use the gas separation membrane and CRDS system joint detection system to measure the changes in gas concentration of single standard gas, mixed gas and human exhaled gas before and after adding the gas separation membrane, verify the coupling and adaptation degree of the gas separation membrane and the CRDS system, and selectively and accurately separate the interfering gas at the ultra-trace level (ppbv) without introducing other gas interferences; S3: Development of a breath analysis system for lung cancer screening based on separation-sensing; a. Quasi-single-mode high pulse frequency laser light source; b, two sample chambers treated with silanization and inertness; c. High reflectivity mirror; d. Photoelectric detection module; e. Data processing module; f. Online measurement and sampling equipment based on gas separation membrane.

2. According to claim 1, a lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy is characterized in that: In step 1, there are two target gases, namely, styrene (C8H8) and ethylbenzene (C8H10), and the interfering gas is acetone.

3. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1 is characterized in that: The properties according to which the hollow fiber membrane and the modified functional layer are prepared in step 1 are: polarity, hydrophilicity and hydrophobicity and analytical size.

4. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1 is characterized in that: In step 1, the thickness and density of the modified functional layer are optimized by studying the membrane structure and performance, so as to achieve accurate separation of the target gas and the interfering gas; the gas separation performance of the modified membrane is evaluated by using the PTR-MS system, and the preparation process of the separation membrane is further optimized according to the detection characteristics of CRDS, so as to prepare a separation membrane for separating ultra-trace (ppbv) level interfering gases.

5. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1, characterized in that: The directions of measuring the performance of the CRDS system in step 2 are divided into: the stability of the operation of the CRDS system, the detection limit of the CRDS system, the linearity of the CRDS system, the repeatability of the CRDS system, the response speed of the CRDS system and the accuracy of the CRDS system. Testing from multiple angles can confirm the overall effect of the CRDS system.

6. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1, characterized in that: The single standard gas detected in step 2 is: target gas and interfering gas; the mixed gas is: a mixture of target gas and interfering gas.

7. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1, characterized in that: In step 2, exhaled breath is used as a sample and compared with the gold standard GC-MS for trace gas detection to verify the accuracy of the combined system.

8. The lung cancer breath trace detection system coupled with membrane separation and cavity ring-down spectroscopy according to claim 1, characterized in that: In step 3, the frequency of the quasi-single-mode high-pulse-frequency laser light source needs to be greater than one kilohertz, that is, >1 kHz; and the reflectivity of the high-reflectivity reflector is >99.985%.

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